CN110951604B - Rotary device for real-time quantitative gene amplification fluorescence detection - Google Patents
Rotary device for real-time quantitative gene amplification fluorescence detection Download PDFInfo
- Publication number
- CN110951604B CN110951604B CN201911422669.9A CN201911422669A CN110951604B CN 110951604 B CN110951604 B CN 110951604B CN 201911422669 A CN201911422669 A CN 201911422669A CN 110951604 B CN110951604 B CN 110951604B
- Authority
- CN
- China
- Prior art keywords
- sample
- photon
- test tube
- real
- gene amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
The invention relates to a rotating device for real-time quantitative gene amplification fluorescent detection, and belongs to the field of real-time quantitative gene amplification fluorescent detection. The invention provides a rotating device for real-time quantitative gene amplification fluorescence detection, which comprises a sample plate, a sample hole, a test tube pressure plate and a photon collecting device; the sample tray is annular, and parallel sample holes are arranged in the circumferential direction of the sample tray; the test tube platen is located above the sample hole, and the photon collection device is located below the sample plate. The rotating device for real-time quantitative gene amplification fluorescence detection provided by the invention has the characteristics of ingenious structure, simple action, strong expansibility, rapid process, stable performance and the like, and is suitable for a Polymerase Chain Reaction (PCR) instrument because the rotating device adopts a movement mode that the sample disk rotates by a central shaft, and the photon collecting device is fixed on a layout structure below the sample disk.
Description
Technical Field
The invention relates to a rotating device for real-time quantitative gene amplification fluorescent detection, and belongs to the field of real-time quantitative gene amplification fluorescent detection.
Background
Real-time fluorescent quantitative PCR (Quantitative Real-time PCR) is a method of measuring the total amount of product after each Polymerase Chain Reaction (PCR) cycle in a DNA amplification reaction using fluorescent chemicals. And quantitatively analyzing the specific DNA sequence in the sample to be tested by an internal reference method or an external reference method.
The polymerase chain reaction technology has extremely high sensitivity, and can simultaneously perform nearly hundred amplification reactions at a time, so that the time and the labor are saved and the efficiency is high. The polymerase chain reaction technique has the following characteristics: as soon as the amount of amplified DNA is extremely small, theoretically one molecule can be used for amplification; secondly, the amplification efficiency is high, the quantity of the target gene is amplified in an exponential form, and the amplification is performed for more than 1000 ten thousand times in a few hours. In the process of multiple polymerase chain reactions, the temperature needs to be increased and decreased for multiple times, and photon collection is also carried out on a sample for multiple times, so that the requirement on a photon collection mode is provided for rapidness and convenience.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a rotating device for real-time quantitative gene amplification fluorescence detection.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: a rotating device for real-time quantitative gene amplification fluorescence detection comprises a sample plate, a sample hole, a test tube pressure plate and a photon collecting device; the sample tray is annular, and parallel sample holes are arranged in the circumferential direction of the sample tray; the test tube platen is located above the sample hole, and the photon collection device is located below the sample plate.
As a preferable embodiment of the rotating device for real-time quantitative gene amplification fluorescence detection of the present invention, the specimen disc is of a rotatable structure.
As a preferable embodiment of the rotating device for real-time quantitative gene amplification fluorescence detection of the present invention, the specimen tray, the plurality of specimen holes and the test tube platen are integrally combined, and the rotating device is positioned at the center of the specimen tray and rotates around a rotation axis.
The appearance of the sample tray is circular, a plurality of sample holes are circumferentially distributed on the sample tray, test tubes are placed in the sample holes, and the test tube pressure plate covers the upper part of the test tubes. The sample tray, the test tubes and the test tube pressure plates are combined into a whole, the rotary shaft at the center of the sample tray is used as the axle center to perform rotary motion, the photon collecting device is positioned below the sample tray, and the photon collecting hole is opposite to the bottom of the test tubes.
As a preferable embodiment of the rotating device for real-time quantitative gene amplification fluorescence detection, the sample hole is of a hollow structure penetrating up and down, the sample hole is inclined at a certain angle to the center of the sample disk, and the cross section of the sample hole is trapezoid with wide top and narrow bottom.
As a preferable implementation mode of the rotating device for real-time quantitative gene amplification fluorescence detection, the test tube pressure plate is round, the size of the test tube pressure plate is equivalent to that of the sample plate, and a handle is further arranged at the top of the test tube pressure plate.
The rotatable sample tray is circumferentially provided with side-by-side sample holes, sample test tubes which are arranged in a circular shape are placed in the sample holes, each test tube rotates around the rotation axis of the sample tray, the photon collecting device is fixed outside the rotating mechanism, fluorescent values of each test tube can be read one by one in the process of rotating the test tube, and photon magnitude of the fluorescent light of each sample in each test tube can be obtained respectively by combining the real-time angle of the rotation of the sample tray.
In the process of collecting the photon quantities of a plurality of test tubes which are arranged in a ring, each test tube is moved in a rotating mode, and when the sample body rotates for one circle, the photon quantity of all test tubes can be collected once, so that the method is simple and quick.
As a preferred implementation mode of the rotating device for real-time quantitative gene amplification fluorescence detection, the photon collecting device is fixed below the sample tray, a collecting port of the photon collecting device faces the sample hole, the collecting port is coaxial with the sample hole, and at least one group of photon collecting devices are arranged.
Because the photon collection device is fixed outside the rotation structure of the sample body and does not belong to the moving part, the installation is convenient, the wire harness layout is simple, and the use process is stable. And for the rotating structure of the sample disk, only one rotating movement mode is adopted, the conditions of linear movement in multiple directions and the like do not exist, the harness is simple to process, the possibility of breakage due to multiple movement times is avoided, and therefore the service life is long, and the performance is stable.
The invention also aims to provide a photon collection method in real-time quantitative gene amplification fluorescence detection, which adopts the rotating device for real-time quantitative gene amplification fluorescence detection to collect photons.
As a preferred embodiment of the photon collection method of the present invention, the photon collection method specifically includes: placing a test tube filled with a sample in the sample hole, covering the test tube pressing plate, starting the sample plate, integrating the sample plate, a plurality of sample holes and the test tube pressing plate into a whole, and taking a rotating shaft as an axle center to perform rotary motion, wherein the photon collecting device below the sample plate performs photon collection.
The photon collection device is placed below the sample tray capable of rotating, when the sample tray drives the plurality of test tubes arranged in a circumferential shape to rotate, the photon collection device below the sample tray performs photon collection, and the photon quantity value of each test tube can be obtained by combining the rotating angle of the sample tray and the photon quantity value received by the photon collection device, so that the samples in each test tube can be analyzed respectively.
According to the photon collection method, a plurality of photon collection devices are placed below the sample tray, so that the purpose of collecting various photons can be achieved, and the expansibility is high.
Compared with the prior art, the invention has the beneficial effects that:
(1) The rotating device for real-time quantitative gene amplification fluorescence detection provided by the invention has the characteristics of ingenious structure, simple action, strong expansibility, rapid process, stable performance and the like, and is suitable for a Polymerase Chain Reaction (PCR) due to the adoption of a moving mode that a sample plate rotates by a central shaft, and the photon collecting device is fixed on a layout structure below the sample plate;
(2) The invention also provides a photon collection method in real-time quantitative gene amplification fluorescence detection, wherein in the collection process of photon quantity of a plurality of test tubes which are arrayed in a ring shape, each test tube is moved in a rotating mode, and when a sample body rotates for one circle, the photon quantity of all test tubes can be collected once, so that the method is simple and rapid.
Drawings
FIG. 1 is a schematic diagram of a rotating device for real-time quantitative gene amplification fluorescence detection; in the figure, 41, a sample tray; 42 sample wells with test tubes; 43. test tube pressure plate; 44. photon collection device.
FIG. 2 is a top view of a rotary device for real-time quantitative gene amplification fluorescence detection provided by the invention.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific examples.
Example 1
The embodiment is a rotating device for real-time quantitative gene amplification fluorescence detection, the structural schematic diagram of which is shown in fig. 1, and the rotating device comprises a sample plate 41, a sample hole 42, a test tube pressure plate 43 and a photon collecting device 44; the sample plate 41 is annular, and has a plurality of sample holes 42 arranged in parallel in the circumferential direction; the cuvette platen 43 is located above the specimen aperture 42, and the photon collection device 44 is located below the specimen tray 41.
The specimen tray 41 is a rotatable structure; the sample tray 41, the plurality of sample holes 42 and the test tube platen 43 are integrally combined, and are positioned at the center of the sample tray 41 and perform rotary motion with a rotary shaft as an axis; the sample hole 42 has a hollow structure penetrating vertically, the sample hole 42 is inclined at a certain angle to the center of the sample plate 41, and the cross section of the sample hole 42 is in a trapezoid shape with a wider upper part and a narrower lower part; the test tube pressing plate 43 is round, the size of the test tube pressing plate is equal to that of the test tube pressing plate 41, and a handle is arranged at the top of the test tube pressing plate 41; the photon collecting device 44 is fixed below the sample tray 41, a collecting port of the photon collecting device 44 faces the sample hole 42, the collecting port is coaxial with the sample hole 42, and at least one group of photon collecting devices 44 are arranged.
The outer shape of the specimen tray 41 is circular, a plurality of specimen holes 42 are circumferentially distributed on the specimen tray 41, test tubes are placed in the specimen holes 42, and a test tube platen 43 is covered over the test tubes. The sample tray 41, the plurality of sample holes 42 and the test tube pressure plate 43 are combined into a whole, a rotation shaft positioned at the center of the sample tray 41 is taken as an axle center to perform rotary motion, the two photon collecting devices 44 are positioned below the sample tray 41, the photon collecting holes of the photon collecting devices 44 are opposite to the bottoms of the test tubes placed in the sample holes 42, when the sample tray 41 drives all the test tubes to perform rotary motion, the photon collecting devices 44 can respectively read the fluorescence photon values of the samples in the test tubes, and the photon value of the samples in the test tubes can be obtained by combining the real-time angle value of the rotation of the sample tray 41 and the photon value received by the photon collecting devices 44 at the moment.
The top view of the rotating device for real-time quantitative gene amplification fluorescence detection provided by the embodiment is shown in fig. 2.
In the photon collection method in real-time quantitative gene amplification fluorescence detection of this embodiment, a test tube containing a sample is placed in the sample hole 42, the test tube platen 43 is covered, the sample plate 41 is started, the sample plate 41, the plurality of sample holes 42 and the test tube platen 43 are combined into a whole, a rotation shaft is used as an axis for rotation, and the photon collection device 44 positioned below the sample plate 41 performs photon collection. The photon collection device 44 is placed below the sample tray 41 capable of performing rotation, when the sample tray 41 drives a plurality of test tubes arranged in a circular shape to rotate, the photon collection device 44 below performs photon collection, and the photon quantity value of each test tube can be obtained by combining the rotation angle of the sample tray 41 and the photon quantity value received by the photon collection device 44, so as to analyze samples in each test tube.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.
Claims (4)
1. The rotating device for real-time quantitative gene amplification fluorescence detection is characterized by comprising a sample plate, a sample hole, a test tube pressure plate and a photon collecting device; the sample tray is annular, and parallel sample holes are arranged in the circumferential direction of the sample tray; the test tube pressure plate is positioned above the sample hole, the size of the test tube pressure plate is equal to that of the sample plate, and the photon collecting device is positioned below the sample plate; the sample hole is of a hollow structure penetrating up and down, the sample hole is inclined to the center of the sample disc by a certain angle, and the cross section of the sample hole is trapezoid with wide upper part and narrow lower part; the photon collection device is fixed below the sample tray, a collection port of the photon collection device is opposite to the sample hole, the collection port is coaxial with the sample hole, and at least one group of photon collection devices are arranged; the sample tray is of a rotatable structure; the sample tray, the plurality of sample holes and the test tube platen are integrated into a whole and positioned at the center of the sample tray, and rotate around the rotation shaft as the axis.
2. The rotary device for real-time quantitative gene amplification fluorescence detection according to claim 1, wherein the test tube platen is circular, and a handle is further arranged at the top of the test tube platen.
3. A photon collection method in real-time quantitative gene amplification fluorescence detection, characterized in that the photon collection is performed by using the rotating device for real-time quantitative gene amplification fluorescence detection according to any one of claims 1-2.
4. A method of photon collection according to claim 3, wherein the method of photon collection is specifically: placing a test tube filled with a sample in the sample hole, covering the test tube pressing plate, starting the sample plate, integrating the sample plate, a plurality of sample holes and the test tube pressing plate into a whole, and taking a rotating shaft as an axle center to perform rotary motion, wherein the photon collecting device below the sample plate performs photon collection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911422669.9A CN110951604B (en) | 2019-12-31 | 2019-12-31 | Rotary device for real-time quantitative gene amplification fluorescence detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911422669.9A CN110951604B (en) | 2019-12-31 | 2019-12-31 | Rotary device for real-time quantitative gene amplification fluorescence detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110951604A CN110951604A (en) | 2020-04-03 |
| CN110951604B true CN110951604B (en) | 2023-07-21 |
Family
ID=69985451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911422669.9A Active CN110951604B (en) | 2019-12-31 | 2019-12-31 | Rotary device for real-time quantitative gene amplification fluorescence detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110951604B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1588004A (en) * | 2004-07-09 | 2005-03-02 | 姚建垣 | Two-way rotary hot wind exchanging type real time fluorescent quantitative poly enzyme chain reaction analyzer |
| CN2715145Y (en) * | 2004-07-09 | 2005-08-03 | 北京英贤仪器有限公司 | Hot air bath real-time fluorescence quantitative gene amplification analyzer |
| CN101021477A (en) * | 2007-03-07 | 2007-08-22 | 博奥生物有限公司 | Hot air circulating temperature controlling real-time fluorescent quantitative PCR detector |
| JP2011062197A (en) * | 2010-09-06 | 2011-03-31 | Seiko Epson Corp | Quantitative method for biological sample |
| JP2014023442A (en) * | 2012-07-25 | 2014-02-06 | Hitachi High-Technologies Corp | Genetic testing device, genetic testing reagent and genetic testing method |
| CN107389915A (en) * | 2017-08-15 | 2017-11-24 | 广州源起健康科技有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
-
2019
- 2019-12-31 CN CN201911422669.9A patent/CN110951604B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1588004A (en) * | 2004-07-09 | 2005-03-02 | 姚建垣 | Two-way rotary hot wind exchanging type real time fluorescent quantitative poly enzyme chain reaction analyzer |
| CN2715145Y (en) * | 2004-07-09 | 2005-08-03 | 北京英贤仪器有限公司 | Hot air bath real-time fluorescence quantitative gene amplification analyzer |
| CN101021477A (en) * | 2007-03-07 | 2007-08-22 | 博奥生物有限公司 | Hot air circulating temperature controlling real-time fluorescent quantitative PCR detector |
| JP2011062197A (en) * | 2010-09-06 | 2011-03-31 | Seiko Epson Corp | Quantitative method for biological sample |
| JP2014023442A (en) * | 2012-07-25 | 2014-02-06 | Hitachi High-Technologies Corp | Genetic testing device, genetic testing reagent and genetic testing method |
| CN107389915A (en) * | 2017-08-15 | 2017-11-24 | 广州源起健康科技有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110951604A (en) | 2020-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3547547A (en) | Analytical photometer with means for measuring,holding and transferring discrete liquid volumes and method of use thereof | |
| JP3626956B2 (en) | Polycapillary fluorescence detection system | |
| CN109337793B (en) | Full-automatic nucleic acid extraction detecting system | |
| CN102618439B (en) | Deoxyribonucleic acid (DNA) fragment amplification and quantitative detection system based on closed reactors | |
| CN109082422B (en) | Full-automatic PCR nucleic acid extraction detection device | |
| CN1226966A (en) | Catalyst testing process and system | |
| RU2013126790A (en) | LATERAL ANALYTICAL DEVICES FOR USE IN THE CLINICAL DIAGNOSTIC DEVICE AND CONFIGURATION OF THE APPROPRIATE CLINICAL DIAGNOSTIC DEVICE | |
| JP2007530039A (en) | Capillary with transparent filter | |
| JP2010506583A (en) | Nucleic acid amplification and detection device | |
| JPH11304666A (en) | Sample handling tool and method for using same | |
| CN110951604B (en) | Rotary device for real-time quantitative gene amplification fluorescence detection | |
| CN111751539A (en) | High-flux vertical flow immune test paper analysis microarray | |
| CN113444773B (en) | Method and kit for detecting tick pathogen nucleic acid based on liquid chip | |
| JP2007534936A (en) | A device to analyze the interaction between target and probe molecules | |
| CN201517993U (en) | Micro-fluidic chip detecting device based on photoacoustic technique | |
| CN106442069B (en) | Automatic proportioning sample injection mechanism | |
| CN210394313U (en) | Fan-shaped nucleic acid multi-joint detection device | |
| CN201527430U (en) | Biochip analyzer | |
| CN217112372U (en) | High-flux full-automatic immune luminescence analysis system | |
| CN215140039U (en) | Porous quantitative sample injector | |
| CN202201884U (en) | Kit for cell biotechnology | |
| US20220299441A1 (en) | Detection method and detection system using suspension liquid biochip | |
| CN210604385U (en) | Rapid pesticide residue detection system based on flower-shaped nano silver substrate | |
| CN217112086U (en) | Reagent and sample automatic measure and cup mechanism that falls in reaction cup | |
| US11529628B2 (en) | Automated immunoassay device and method using large magnetic particle complex |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |