CN110951604A - Rotating device for real-time quantitative gene amplification fluorescence detection - Google Patents
Rotating device for real-time quantitative gene amplification fluorescence detection Download PDFInfo
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- CN110951604A CN110951604A CN201911422669.9A CN201911422669A CN110951604A CN 110951604 A CN110951604 A CN 110951604A CN 201911422669 A CN201911422669 A CN 201911422669A CN 110951604 A CN110951604 A CN 110951604A
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Abstract
The invention relates to a rotating device for real-time quantitative gene amplification fluorescence detection, belonging to the field of real-time quantitative gene amplification fluorescence detection. The invention provides a rotary device for real-time quantitative gene amplification fluorescence detection, which comprises a detection body disc, detection body holes, a test tube pressure plate and a photon collecting device, wherein the detection body holes are arranged on the detection body disc; the body testing disc is annular, and body testing holes are arranged side by side in the circumferential direction of the body testing disc; the test tube pressure plate is positioned above the detection body hole, and the photon collecting device is positioned below the detection body plate. The rotating device for real-time quantitative gene amplification fluorescence detection provided by the invention adopts the movement mode that the sample tray rotates around the central shaft, and the photon collecting device is fixed on the layout structure below the sample tray, so that the rotating device has the characteristics of ingenious structure, simple action, strong expansibility, rapid process, stable performance and the like, and is suitable for being used on a polymerase chain reaction instrument (PCR instrument).
Description
Technical Field
The invention relates to a rotating device for real-time quantitative gene amplification fluorescence detection, belonging to the field of real-time quantitative gene amplification fluorescence detection.
Background
Real-time fluorescent Quantitative PCR (Quantitative Real-time PCR) is a method for measuring the total amount of products after each Polymerase Chain Reaction (PCR) cycle by using fluorescent chemical substances in DNA amplification reaction. A method for quantitatively analyzing a specific DNA sequence in a sample to be detected by an internal reference method or an external reference method.
The polymerase chain reaction technology not only has extremely high sensitivity, but also can simultaneously carry out nearly hundreds of amplification reactions at one time, thereby saving time and labor and having high efficiency. The polymerase chain reaction technique has the following characteristics: firstly, the required amount of amplified DNA is extremely small, theoretically one molecule can be used for amplification; secondly, the amplification efficiency is high, the quantity of the target gene is amplified in an exponential mode, and the amplification is more than 1000 ten thousand times within a few hours. In the process of multiple polymerase chain reactions, temperature rise and temperature reduction are needed for multiple times, and photon collection is also needed for multiple times, so that the requirement on a photon collection mode is provided more quickly and conveniently.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a rotary device for real-time quantitative gene amplification fluorescence detection.
In order to achieve the purpose, the invention adopts the technical scheme that: a rotary device for real-time quantitative gene amplification fluorescence detection comprises a detection body disc, detection body holes, a test tube pressure plate and a photon collecting device; the body testing disc is annular, and body testing holes are arranged side by side in the circumferential direction of the body testing disc; the test tube pressure plate is positioned above the detection body hole, and the photon collecting device is positioned below the detection body plate.
In a preferred embodiment of the rotary device for real-time quantitative gene amplification fluorescent detection according to the present invention, the body plate has a rotatable structure.
In a preferred embodiment of the rotary apparatus for real-time quantitative gene amplification fluorescent detection according to the present invention, the sample plate is integrally combined with the plurality of sample wells and the test tube platen, is positioned at the center of the sample plate, and is rotated about a rotation axis.
The appearance of the test body disc is in a circular ring shape, a plurality of test body holes are distributed on the circumference of the test body disc, test tubes are placed in the test body holes, and the test tube pressing disc covers the upper portion of each test tube. The test body tray is integrated with a plurality of test tubes and test tube pressure plates, and rotates around a rotating shaft at the center of the test body tray, the photon collecting device is arranged below the test body tray, and the photon collecting holes are opposite to the bottoms of the test tubes.
In a preferred embodiment of the rotary apparatus for real-time quantitative gene amplification fluorescent detection according to the present invention, the specimen hole has a hollow structure penetrating vertically, the specimen hole is inclined at a predetermined angle toward the center of the specimen tray, and the cross section of the specimen hole has a trapezoidal shape with a wide top and a narrow bottom.
In a preferred embodiment of the rotary apparatus for real-time quantitative gene amplification fluorescence detection according to the present invention, the test tube platen is circular and has a size corresponding to that of the sample plate, and a handle is further provided on a top of the test tube platen.
On rotatable body dish, its circumference is equipped with the body hole side by side, places the ring shape body test tube of arranging in the body hole, and each test tube rotates with the rotation axis center of body dish, and photon collection system fixes motionless outside rotary mechanism, can read the fluorescence value of each test tube one by one at the rotatory in-process of test tube, combines the rotatory real-time angle of body dish, can obtain the fluorescence's of each intraductal body of examining the body photon quantity value respectively.
In the process of collecting the photon quantities of the plurality of test tubes which are arranged in a ring shape, because the test tubes are moved in a rotating mode, when the sample disk rotates for a circle, the photon quantities of all the test tubes can be collected for one time, and the method is simple and quick.
As a preferred embodiment of the rotating apparatus for real-time quantitative gene amplification fluorescence detection according to the present invention, the photon collecting apparatus is fixed below the sample tray, a collecting port of the photon collecting apparatus faces the sample hole, the collecting port is coaxial with the sample hole, and at least one group of the photon collecting apparatus is provided.
The photon collecting device is fixed outside the rotating structure of the sample disk and does not belong to a moving part, so that the installation is convenient, the wiring harness layout is simple, and the using process is stable. In contrast, in the rotary structure of the sample disk, only one rotary motion mode is provided, so that the situations of linear motion in multiple directions and the like do not exist, the wire harness is simple to process, the possibility of breakage caused by a large number of motions does not occur, the service life is long, and the performance is stable.
The invention also aims to provide a photon collection method in the real-time quantitative gene amplification fluorescence detection, which adopts the rotating device for the real-time quantitative gene amplification fluorescence detection to collect photons.
As a preferred embodiment of the photon collection method of the present invention, the photon collection method specifically includes: the test tube that will be equipped with the sample is placed in examine body hole covers the test tube pressure disk, starts examine body dish, examine body dish and a plurality of examine body hole reaches test tube pressure disk combines into an organic whole, uses the rotation axis to do rotary motion as the axle center, is located examine body dish below photon collection device carries out photon and gathers.
The photon collecting device is arranged below the sample tray capable of rotating, when the sample tray drives a plurality of test tubes arranged in a circumferential shape to rotate, the photon collecting device below the sample tray collects photons, and photon quantity values of the test tubes can be obtained by combining the rotating angle of the sample tray and photon quantity values received by the photon collecting device, so that samples in the test tubes can be analyzed respectively.
According to the photon collection method, the purpose of collecting various photons can be achieved by arranging the photon collection devices below the sample tray, and the expansibility is strong.
Compared with the prior art, the invention has the beneficial effects that:
(1) the rotating device for real-time quantitative gene amplification fluorescence detection provided by the invention adopts the movement mode that the sample tray rotates around the central shaft, and the photon collecting device is fixed on the layout structure below the sample tray, so that the rotating device has the characteristics of ingenious structure, simple action, strong expansibility, rapid process, stable performance and the like, and is suitable for being used on a Polymerase Chain Reaction (PCR);
(2) the invention also provides a photon collection method in real-time quantitative gene amplification fluorescence detection, in the collection process of the photon quantities of a plurality of test tubes which are arranged in a ring shape, because the rotation mode is adopted to move each test tube, when the sample disk rotates for one circle, the photon quantities of all the test tubes can be collected for one time, and the method is simple and quick.
Drawings
FIG. 1 is a schematic structural diagram of a rotary apparatus for real-time quantitative gene amplification fluorescence detection provided by the present invention; in the figure, 41, a sample tray; 42 a sample well in which a test tube is placed; 43. a test tube platen; 44. photon collection device.
FIG. 2 is a top view of a rotary apparatus for real-time quantitative fluorescent detection of gene amplification according to the present invention.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
The present embodiment is a rotary apparatus for real-time quantitative gene amplification fluorescence detection, the structural schematic diagram of which is shown in fig. 1, and the rotary apparatus includes a sample tray 41, sample holes 42, a test tube platen 43, and a photon collecting device 44; the body disc 41 is annular, and body holes 42 which are arranged side by side are formed in the circumferential direction of the body disc; the test tube platen 43 is located above the subject hole 42, and the photon collection device 44 is located below the subject disc 41.
The body disc 41 is a rotatable structure; the test body disc 41 is integrated with the plurality of test body holes 42 and the test tube platen 43, is positioned at the center of the test body disc 41, and performs rotary motion by taking a rotary shaft as an axis; the body hole 42 is a hollow structure which penetrates through the body hole from top to bottom, the body hole 42 inclines to the center of the body disc 41 by a certain angle, and the cross section of the body hole 42 is in a trapezoid shape with a wide top and a narrow bottom; the test tube pressing plate 43 is circular, the size of the test tube pressing plate is equivalent to that of the test body plate 41, and the top of the test tube pressing plate 41 is also provided with a handle; the photon collecting device 44 is fixed below the object disc 41, a collecting port of the photon collecting device 44 is over against the object hole 42, the collecting port is coaxial with the object hole 42, and at least one group of photon collecting devices 44 is provided.
The sample disk 41 has a ring-shaped outer shape, a plurality of sample holes 42 are circumferentially distributed on the sample disk 41, test tubes are placed in the sample holes 42, and the test tube platen 43 is placed over the test tubes. The test body disc 41 is integrated with a plurality of test body holes 42 and a test tube pressure disc 43, and can rotate around a rotating shaft at the center of the test body disc 41, two photon collecting devices 44 are arranged below the test body disc 41, the photon collecting holes of the photon collecting devices 44 are right opposite to the bottoms of the test tubes arranged in the test body holes 42, when the test body disc 41 drives all the test tubes to rotate, the photon collecting devices 44 can respectively read the fluorescence photon quantity values of the test bodies in the test tubes, and the photon quantity values of the test bodies in the test tubes can be obtained by combining the real-time angle value when the test body disc 41 rotates and the photon quantity values received by the photon collecting devices 44 at the moment, so that samples of each test tube 42 can be analyzed respectively.
FIG. 2 is a top view of the rotary apparatus for real-time quantitative fluorescent detection of gene amplification according to this example.
In the photon collection method in the real-time quantitative gene amplification fluorescence detection according to the present embodiment, a test tube containing a sample is placed in the test body hole 42, the test tube platen 43 is covered, the test body disk 41 is started, the test body disk 41 is integrated with the plurality of test body holes 42 and the test tube platen 43, and performs a rotational motion with the rotation axis as the axis, and the photon collection device 44 located below the test body disk 41 performs photon collection. The photon collecting device 44 is disposed below the sample tray 41 capable of rotating, when the sample tray 41 drives a plurality of test tubes arranged in a circle to rotate, the photon collecting device 44 below performs photon collection, and photon quantity values of the test tubes can be obtained by combining the rotating angle of the sample tray 41 and photon quantity values received by the photon collecting device 44, so as to analyze samples in each test tube.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (8)
1. A rotary device for real-time quantitative gene amplification fluorescence detection is characterized by comprising a detection body disc, detection body holes, a test tube pressure disc and a photon collecting device; the body testing disc is annular, and body testing holes are arranged side by side in the circumferential direction of the body testing disc; the test tube pressure plate is positioned above the detection body hole, and the photon collecting device is positioned below the detection body plate.
2. The rotary device for real-time quantitative gene amplification fluorescent detection according to claim 1, wherein the body disk is a rotatable structure.
3. The rotary device for real-time quantitative gene amplification fluorescent detection according to claim 2, wherein the sample plate is integrally combined with the plurality of sample holes and the test tube platen, is located at the center of the sample plate, and is rotated around a rotation axis.
4. The rotary device for real-time quantitative gene amplification fluorescent detection according to claim 1, wherein the sample hole has a hollow structure penetrating vertically, the sample hole is inclined at a certain angle toward the center of the sample tray, and the cross section of the sample hole has a trapezoidal shape with a wide top and a narrow bottom.
5. The rotary device for real-time quantitative gene amplification fluorescent detection according to claim 1, wherein the test tube platen is circular and has a size corresponding to that of the sample tray, and a handle is further provided on the top of the test tube platen.
6. The rotary device for real-time quantitative gene amplification fluorescence detection according to claim 1, wherein the photon collection device is fixed under the sample disk, a collection port of the photon collection device faces the sample hole, the collection port is coaxial with the sample hole, and at least one group of the photon collection devices is provided.
7. A photon collection method in real-time quantitative gene amplification fluorescent detection, characterized in that photon collection is performed by using the rotating device for real-time quantitative gene amplification fluorescent detection according to any one of claims 1 to 6.
8. The photon collection method according to claim 7, wherein the photon collection method is specifically: the test tube that will be equipped with the sample is placed in examine body hole covers the test tube pressure disk, starts examine body dish, examine body dish and a plurality of examine body hole reaches test tube pressure disk combines into an organic whole, uses the rotation axis to do rotary motion as the axle center, is located examine body dish below photon collection device carries out photon and gathers.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911422669.9A CN110951604B (en) | 2019-12-31 | 2019-12-31 | Rotary device for real-time quantitative gene amplification fluorescence detection |
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| CN201911422669.9A CN110951604B (en) | 2019-12-31 | 2019-12-31 | Rotary device for real-time quantitative gene amplification fluorescence detection |
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Citations (6)
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| CN1588004A (en) * | 2004-07-09 | 2005-03-02 | 姚建垣 | Two-way rotary hot wind exchanging type real time fluorescent quantitative poly enzyme chain reaction analyzer |
| CN2715145Y (en) * | 2004-07-09 | 2005-08-03 | 北京英贤仪器有限公司 | Hot air bath real-time fluorescence quantitative gene amplification analyzer |
| CN101021477A (en) * | 2007-03-07 | 2007-08-22 | 博奥生物有限公司 | Hot air circulating temperature controlling real-time fluorescent quantitative PCR detector |
| JP2011062197A (en) * | 2010-09-06 | 2011-03-31 | Seiko Epson Corp | Quantitative method for biological sample |
| JP2014023442A (en) * | 2012-07-25 | 2014-02-06 | Hitachi High-Technologies Corp | Genetic testing device, genetic testing reagent and genetic testing method |
| CN107389915A (en) * | 2017-08-15 | 2017-11-24 | 广州源起健康科技有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
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2019
- 2019-12-31 CN CN201911422669.9A patent/CN110951604B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1588004A (en) * | 2004-07-09 | 2005-03-02 | 姚建垣 | Two-way rotary hot wind exchanging type real time fluorescent quantitative poly enzyme chain reaction analyzer |
| CN2715145Y (en) * | 2004-07-09 | 2005-08-03 | 北京英贤仪器有限公司 | Hot air bath real-time fluorescence quantitative gene amplification analyzer |
| CN101021477A (en) * | 2007-03-07 | 2007-08-22 | 博奥生物有限公司 | Hot air circulating temperature controlling real-time fluorescent quantitative PCR detector |
| JP2011062197A (en) * | 2010-09-06 | 2011-03-31 | Seiko Epson Corp | Quantitative method for biological sample |
| JP2014023442A (en) * | 2012-07-25 | 2014-02-06 | Hitachi High-Technologies Corp | Genetic testing device, genetic testing reagent and genetic testing method |
| CN107389915A (en) * | 2017-08-15 | 2017-11-24 | 广州源起健康科技有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
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