CN215365745U - Rotatory fluorescence sampling structure of multichannel - Google Patents
Rotatory fluorescence sampling structure of multichannel Download PDFInfo
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- CN215365745U CN215365745U CN202022915301.0U CN202022915301U CN215365745U CN 215365745 U CN215365745 U CN 215365745U CN 202022915301 U CN202022915301 U CN 202022915301U CN 215365745 U CN215365745 U CN 215365745U
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Abstract
The utility model discloses a multi-channel rotating fluorescence sampling structure, which comprises: the optical fiber sampling device comprises an annular optical fiber fixing disc and an annular sampling rotary disc which is concentric with the annular optical fiber fixing disc, wherein optical fibers are fixed in the disc surface of the optical fiber fixing disc, the position of the sampling rotary disc, corresponding to the optical fibers, is provided with a fluorescence module, the optical fiber fixing disc and the sampling rotary disc are coaxially and movably connected through a crossed roller bearing and are fixedly connected with bearing rings where the optical fibers are located respectively, the circle center position of the sampling rotary disc is provided with a rotating shaft and is fixedly connected with the sampling rotary disc, the optical fiber fixing disc is provided with a motor, and an output shaft of the motor is linked with the rotating shaft. The structure designs the transmission structure between the optical fiber fixed disc and the sampling turntable by using the crossed roller bearing, thereby improving the rotating stability of the sampling turntable and enabling the fluorescent signal detection to be more stable; meanwhile, the elastic coupling is arranged at the output end of the motor, so that the running stability of the whole structure is further improved.
Description
Technical Field
The utility model relates to the technical field of biological detection, in particular to a multi-channel rotating fluorescence sampling structure.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technology for amplifying and amplifying specific DNA fragments, which can be regarded as special DNA amplification outside organisms, and a temperature cycle period is formed by several steps of reactions such as high-temperature denaturation, low-temperature annealing, moderate-temperature extension and the like, so that the purpose can be rapidly amplified, and the PCR amplification technology has the characteristics of strong specificity, high sensitivity, simplicity and convenience in operation, time saving and the like, and is a major innovation of a gene amplification technology.
The general fluorescent quantitative PCR instrument is an instrument for detecting reaction in real time, and comprises a thermal cycler and a fluorescent real-time detection system, wherein the thermal cycler and the fluorescent real-time detection system are provided with two functional modules for ensuring the PCR reaction.
Real-time fluorescent Quantitative PCR (Quantitative Real-time PCR) is a method for quantitatively analyzing a specific DNA sequence in a sample to be detected by an internal reference or external reference method by measuring the total amount of products after each Polymerase Chain Reaction (PCR) cycle by using fluorescent chemical substances in a DNA amplification reaction. Real-time PCR is a Real-time detection of PCR progress by fluorescence signals during PCR amplification.
In the prior art, a motor is directly connected with a sampling turntable to drive a detection module to scan an optical fiber, so that the sample is detected. The structure can cause the operation of the sampling turntable to shake, and when the processing or installation error is large, extrusion sound can be generated in the rotating process, so that the transmitting and receiving stability of the fluorescent signal is influenced.
SUMMERY OF THE UTILITY MODEL
Utility model purpose: in order to overcome the defects of the background art, the utility model discloses a multi-channel rotating fluorescence sampling structure, which realizes the stable rotation of a sampling turntable and achieves the stable detection of a fluorescence signal.
The technical scheme is as follows: the utility model relates to a multi-channel rotating fluorescence sampling structure, which comprises: the optical fiber sampling device comprises an annular optical fiber fixing disc and an annular sampling rotary disc which is concentric with the annular optical fiber fixing disc, wherein optical fibers are fixed in the disc surface of the optical fiber fixing disc, the position of the sampling rotary disc, corresponding to the optical fibers, is provided with a fluorescence module, the optical fiber fixing disc and the sampling rotary disc are coaxially and movably connected through a crossed roller bearing and are fixedly connected with bearing rings where the optical fibers are located respectively, the circle center position of the sampling rotary disc is provided with a rotating shaft and is fixedly connected with the sampling rotary disc, the optical fiber fixing disc is provided with a motor, and an output shaft of the motor is linked with the rotating shaft.
The sampling turntable is coaxially and movably connected with the optical fiber fixing disk through a crossed roller bearing, and simultaneously, the sampling turntable is driven by the motor to coaxially rotate relative to the optical fiber fixing disk, so that the rotation is more stable. The fluorescence module does circular motion and scans the optical fiber at the same time, so that the fluorescence signal detection is more stable.
Specifically, the optical fiber fixing disc and the sampling turntable are respectively fixed with an outer bearing ring and an inner bearing ring of the crossed roller bearing through screws, so that coaxial movable connection is realized.
Furthermore, the output shaft of the motor is connected with the rotating shaft through the elastic coupling, so that the starting force of the motor is weakened, meanwhile, buffering and vibration reduction are realized between the output shaft of the motor and the rotating shaft, and the rotating stability of the sampling turntable is further improved.
Furthermore, the fluorescent modules are uniformly arranged on the surface of the sampling turntable at intervals.
Furthermore, a support plate is fixed on the optical fiber fixing disc, and the motor is arranged on the support plate.
Has the advantages that: compared with the prior art, the utility model has the advantages that: firstly, the structure designs a transmission structure between the optical fiber fixed disc and the sampling turntable by using the crossed roller bearing, so that the rotating stability of the sampling turntable is improved, and the fluorescent signal detection is more stable; meanwhile, the elastic coupling is arranged at the output end of the motor, so that the running stability of the whole structure is further improved.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
fig. 2 is a cross-sectional view of the present invention.
Detailed Description
The technical solution of the present invention is further described below with reference to the accompanying drawings and examples.
A multi-channel rotating fluorescence sampling structure as shown in fig. 1 and 2, comprising: the optical fiber fixed disk of ring shape 1 and rather than the centre of a circle and set up the ring shape sampling carousel 2 at its lower surface, optic fibre 3 inserts in the aperture of 1 quotations of optical fiber fixed disk and fixed, the position that sampling carousel 2 corresponds optic fibre 3 is equipped with fluorescence module 4, and fluorescence module 4's emission and receiving channel are all to optic fibre 3, and fluorescence module 4 is equipped with 6 in this embodiment, sets up at the even interval in sampling carousel 2 quotations.
The optical fiber fixing disc 1 and the sampling turntable 2 are coaxially and movably connected through a cross roller bearing 9, and the optical fiber fixing disc 1 and the sampling turntable 2 are respectively fixed with an outer bearing ring and an inner bearing ring of the cross roller bearing 9 through screws to realize coaxial movable connection.
The centre of a circle position of sampling carousel 2 is equipped with pivot 5, and pivot 5 passes the centre of a circle position of sampling carousel 2 and links firmly with sampling carousel 2, be equipped with a support 8 on the optical fiber fixing disk 1, fix the centre of a circle position at optical fiber fixing disk 1 through the double-screw bolt, be equipped with motor 6 on the support 8, the output shaft of motor 6 passes through elastic coupling 7 linkage with pivot 5.
The movement mode is that the motor 6 drives the elastic coupling 7, the rotating shaft 5 and the sampling turntable 2 to rotate together when working, then the 6 fluorescence modules 4 do circular motion and scan each optical fiber in sequence, and therefore the result of fluorescence detection is achieved.
Claims (5)
1. A multi-channel rotating fluorescence sampling structure, comprising: the optical fiber fixing disc comprises an annular optical fiber fixing disc (1) and an annular sampling rotary disc (2) concentric with the annular optical fiber fixing disc, wherein optical fibers (3) are fixed in the disc surface of the optical fiber fixing disc (1), the position of the sampling rotary disc (2) corresponding to the optical fibers (3) is provided with a fluorescence module (4), the optical fiber fixing disc (1) and the sampling rotary disc (2) are coaxially movably connected through a crossed roller bearing (9) and fixedly connected with bearing rings respectively located at the optical fiber fixing disc and the sampling rotary disc, the circle center position of the sampling rotary disc (2) is provided with a rotating shaft (5) and fixedly connected with the sampling rotary disc (2), a motor (6) is arranged on the optical fiber fixing disc (1), and an output shaft of the motor (6) is linked with the rotating shaft (5).
2. The multi-channel rotating fluorescence sampling structure of claim 1, wherein: the optical fiber fixing disc (1) and the sampling turntable (2) are respectively fixed with an outer bearing ring and an inner bearing ring of the crossed roller bearing (9) through screws, and coaxial movable connection is achieved.
3. The multi-channel rotating fluorescence sampling structure of claim 1, wherein: and an output shaft of the motor (6) is connected with the rotating shaft (5) through an elastic coupling (7).
4. The multi-channel rotating fluorescence sampling structure of claim 1, wherein: the fluorescence modules (4) are uniformly arranged on the surface of the sampling turntable (2) at intervals.
5. The multi-channel rotating fluorescence sampling structure of claim 1, wherein: a support plate (8) is fixed above the optical fiber fixing disc (1), and the motor (6) is arranged on the support plate (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022915301.0U CN215365745U (en) | 2020-12-08 | 2020-12-08 | Rotatory fluorescence sampling structure of multichannel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022915301.0U CN215365745U (en) | 2020-12-08 | 2020-12-08 | Rotatory fluorescence sampling structure of multichannel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215365745U true CN215365745U (en) | 2021-12-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN202022915301.0U Active CN215365745U (en) | 2020-12-08 | 2020-12-08 | Rotatory fluorescence sampling structure of multichannel |
Country Status (1)
| Country | Link |
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| CN (1) | CN215365745U (en) |
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2020
- 2020-12-08 CN CN202022915301.0U patent/CN215365745U/en active Active
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