CN212293598U - Nucleic acid extraction, amplification and detection integrated analyzer - Google Patents
Nucleic acid extraction, amplification and detection integrated analyzer Download PDFInfo
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- CN212293598U CN212293598U CN202021892414.7U CN202021892414U CN212293598U CN 212293598 U CN212293598 U CN 212293598U CN 202021892414 U CN202021892414 U CN 202021892414U CN 212293598 U CN212293598 U CN 212293598U
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Abstract
The invention discloses an integrated analyzer for nucleic acid extraction, amplification and detection, which comprises: the magnet rotates around the side wall of the test tube to drive magnetic beads in the test tube to sequentially pass through different areas in the test tube to realize nucleic acid extraction and cleaning, and finally enters the reaction area; the amplification module is provided with a plurality of test tube holes for holding a plurality of test tubes and provides conditions for the nucleic acid amplification reaction in the test tube reaction area through temperature change; one or more fluorescence detection modules for detecting nucleic acid amplification signals within the reaction zones of the cuvette. The whole extraction and amplification process is carried out under a closed condition, so that the environmental pollution is effectively avoided; the invention fully automatically finishes the extraction, amplification and detection of the whole experimental process through equipment, and greatly simplifies the experimental operation difficulty.
Description
Technical Field
The invention belongs to the field of in-vitro diagnosis nucleic acid detection, and relates to an integrated analyzer for nucleic acid extraction, amplification and detection.
Background
The principle of magnetic bead method for extracting nucleic acid is that according to the same principle as that of silica gel membrane centrifugal column, the surface of superparamagnetic nano-particles is modified and surface-modified by using nano-technology to prepare superparamagnetic silica nano-magnetic beads. The magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface. By utilizing the superparamagnetism of the nanometer magnetic microspheres coated by the silicon dioxide, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate and the like) and an external magnetic field, DNA and RNA can be separated from samples such as blood, animal tissues, food, pathogenic microorganisms and the like, and the magnetic microsphere can be applied to multiple fields such as clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, molecular biology research and the like.
The nucleic acid amplification detection technology is a process of providing in vitro amplification conditions for nucleic acid fragments, enabling the nucleic acid fragments to be amplified in an exponential manner in a large quantity, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and obtaining a nucleic acid amplification result by processing and analyzing the fluorescent signal.
The steps of cracking, cleaning, eluting, amplifying and the like are included in the process of nucleic acid extraction and amplification detection, the current mainstream technology must be carried out step by step in a standard laboratory, the whole process is easy to pollute the sample nucleic acid, the operation difficulty is high, the requirement on operators is high, and the popularization of the nucleic acid detection technology is not facilitated.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides the integrated analyzer for nucleic acid extraction, amplification and detection, which greatly simplifies the operation difficulty from nucleic acid extraction to amplification, improves the detection efficiency, effectively avoids the pollution of the environment to a sample and improves the detection accuracy.
The technical scheme of the invention is as follows: an integrated analyzer for nucleic acid extraction, amplification and detection, comprising:
the magnet rotates around the side wall of the test tube to drive magnetic beads in the test tube to sequentially pass through different areas in the test tube to realize nucleic acid extraction and cleaning, and finally enters the reaction area;
the amplification module is provided with a plurality of test tube holes for holding a plurality of test tubes and provides conditions for the nucleic acid amplification reaction in the test tube reaction zone through temperature change;
one or more fluorescence detection modules for detecting a nucleic acid amplification signal within the cuvette reaction area.
In the aforementioned integrated analyzer for nucleic acid extraction, amplification and detection, the magnet is fixed on the rotating seat, and the rotating seat is fixed with the rotating shaft of the motor.
In the integrated analyzer for nucleic acid extraction, amplification and detection, the amplification module is tightly attached to the semiconductor refrigeration sheet.
In the aforementioned integrated analyzer for nucleic acid extraction, amplification and detection, the fluorescence detection module includes a Y-shaped optical detection fiber, an excitation light filter, an emission light filter, and a photodetector.
In the aforementioned integrated analyzer for nucleic acid extraction, amplification and detection, the test tube hole of the amplification module is provided with a detection hole for detecting a nucleic acid amplification signal in the test tube.
In the aforementioned integrated analyzer for nucleic acid extraction, amplification and detection, the fluorescence detection module detects the nucleic acid amplification signals in the plurality of test tubes by translation or rotation along the detection holes of the amplification module.
In the above-mentioned integrated analyzer for nucleic acid extraction, amplification and detection, the other surface of the semiconductor refrigeration sheet is tightly attached to the heat sink.
Compared with the prior art, the gain effect of the invention is as follows: the invention carries out the whole extraction and amplification process under a closed condition, thereby effectively avoiding environmental pollution. The invention fully automatically finishes the extraction, amplification and detection of the whole experimental process through equipment, and greatly simplifies the experimental operation difficulty.
Drawings
Fig. 1 is a cross-sectional view of the present invention.
Fig. 2 is a perspective view of the present invention.
Fig. 3 is a sectional view taken along the line a-a in fig. 1.
FIG. 4 is a cross-sectional view of a fluorescence detection module of the present invention.
FIG. 5 is a perspective view of an amplification module of the present invention.
The labels in the figures are: 1-magnet, 2-test tube, 3-amplification module, 4-fluorescence detection module, 5-semiconductor refrigeration piece, 6-radiator, 7-rotary seat, 8-motor, 9-linear motor, 401-Y type optical detection fiber, 403-exciting light, 402-exciting light filter, 404-emission light filter and 405-photoelectric detector.
Detailed Description
The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
Examples are given. An integrated analyzer for nucleic acid extraction, amplification and detection, as shown in FIGS. 1 to 3, comprising:
the magnet 1 rotates around the side wall of the test tube 2 to drive magnetic beads in the test tube to sequentially pass through different areas in the test tube to realize nucleic acid extraction and cleaning, and finally enters a reaction area;
the amplification module 3 is provided with 3 test tube holes for holding 3 test tubes, and the amplification module 3 provides conditions for the nucleic acid amplification reaction in the reaction area of the test tube 2 through temperature change;
a fluorescence detection module 4, wherein the fluorescence detection module 4 is used for detecting the nucleic acid amplification signal in the reaction area of the test tube 2.
The magnet 1 is fixed on the rotating seat 7, and the rotating seat 7 is fixed with the rotating shaft of the motor 8.
The amplification module 3 is tightly attached to the semiconductor refrigerating sheet 5, and the amplification module 3 is heated and refrigerated through the semiconductor refrigerating sheet 5 to control the temperature.
As shown in fig. 4, the fluorescence detection module includes a Y-shaped optical detection fiber 401, an excitation light 403, an excitation light filter 402, an emission light filter 404, and a photodetector 405.
As shown in FIG. 5, the test tube wells of the amplification module 3 are provided with detection wells for detecting nucleic acid amplification signals in the test tubes 2.
As shown in FIG. 2, the fluorescence detection module 4 is translated along the detection holes of the amplification module 3 by the linear motor 9 to detect the nucleic acid amplification signals in a plurality of test tubes.
The other surface of the semiconductor refrigerating sheet 5 is tightly attached to the radiator 6 to dissipate redundant heat.
It is to be understood that: although the present invention has been described in detail, the present invention is only for the purpose of illustration and not limitation, and any invention that does not depart from the spirit of the present invention falls within the scope of the present invention.
Claims (7)
1. An integrated analyzer for nucleic acid extraction, amplification and detection, comprising:
the magnet rotates around the side wall of the test tube to drive magnetic beads in the test tube to sequentially pass through different areas in the test tube to realize nucleic acid extraction and cleaning, and finally enters the reaction area;
the amplification module is provided with a plurality of test tube holes for holding a plurality of test tubes and provides conditions for the nucleic acid amplification reaction in the test tube reaction zone through temperature change;
one or more fluorescence detection modules for detecting a nucleic acid amplification signal within the cuvette reaction area.
2. The integrated analyzer for nucleic acid extraction, amplification and detection according to claim 1, wherein: the magnet is fixed on the rotating seat, and the rotating seat is fixed with the motor rotating shaft.
3. The integrated analyzer for nucleic acid extraction, amplification and detection according to claim 1, wherein: the amplification module is tightly attached to the semiconductor refrigerating sheet.
4. The integrated analyzer for nucleic acid extraction, amplification and detection according to claim 1, wherein: the fluorescence detection module comprises a Y-shaped optical detection optical fiber, an exciting light optical filter, an emitting light optical filter and a photoelectric detector.
5. The integrated analyzer for nucleic acid extraction, amplification and detection according to claim 1, wherein: and a detection hole is formed in the test tube hole of the amplification module and is used for detecting a nucleic acid amplification signal in the test tube.
6. The integrated analyzer for nucleic acid extraction, amplification and detection according to claim 1, wherein: the fluorescence detection module detects nucleic acid amplification signals in a plurality of test tubes by translation or rotation along the amplification module detection holes.
7. The integrated analyzer for nucleic acid extraction, amplification and detection according to claim 3, wherein: the other side of the semiconductor refrigeration sheet is tightly attached to the radiator.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021892414.7U CN212293598U (en) | 2020-09-03 | 2020-09-03 | Nucleic acid extraction, amplification and detection integrated analyzer |
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| CN202021892414.7U CN212293598U (en) | 2020-09-03 | 2020-09-03 | Nucleic acid extraction, amplification and detection integrated analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112628357A (en) * | 2021-02-15 | 2021-04-09 | 杭州天微基因科技有限公司 | A gear box for nucleic acid extraction |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112628357A (en) * | 2021-02-15 | 2021-04-09 | 杭州天微基因科技有限公司 | A gear box for nucleic acid extraction |
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