CN214496301U - Nucleic acid extraction amplification detection test tube - Google Patents
Nucleic acid extraction amplification detection test tube Download PDFInfo
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- CN214496301U CN214496301U CN202120371467.2U CN202120371467U CN214496301U CN 214496301 U CN214496301 U CN 214496301U CN 202120371467 U CN202120371467 U CN 202120371467U CN 214496301 U CN214496301 U CN 214496301U
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- nucleic acid
- cleaning bin
- tube
- isolation cavity
- acid extraction
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Abstract
The abstract of the specification: the invention discloses a nucleic acid extraction amplification detection test tube, which comprises: the bottom of the pyrolysis liquid pipe is provided with an isolation cavity, a cleaning bin and a reaction pipe; the isolation cavity, the cleaning bin and the reaction tube are horizontally arranged into a circle; the front end of the isolation cavity is communicated with the bottom of the pyrolysis liquid pipe, and the rear end of the isolation cavity is communicated with the front end of the cleaning bin; the rear end of the cleaning bin is communicated with the side wall of the reaction tube; the reaction tube and the cracking liquid tube are mutually separated. The top of the lysis tube is provided with an opening for loading a sample. The top of schizolysis liquid pipe is provided with sealed lid and is used for sealed schizolysis liquid pipe. And the top of the isolation cavity is provided with an isolation cavity injection hole. And a cleaning bin injection pipe is arranged at the top of the cleaning bin. And a cleaning bin exhaust pipe is arranged at the top of the cleaning bin. The top of the reaction tube is provided with a reaction tube injection hole. The invention integrates multiple steps of nucleic acid extraction, cleaning, elution and amplification reaction into a closed test tube, thereby effectively reducing the operation steps and difficulty of nucleic acid detection.
Description
Technical Field
The invention belongs to the field of molecular diagnosis nucleic acid detection, and relates to a nucleic acid extraction amplification detection test tube.
Background
The principle of magnetic bead method for extracting nucleic acid is that according to the same principle as that of silica gel membrane centrifugal column, the surface of superparamagnetic nano-particles is modified and surface-modified by using nano-technology to prepare superparamagnetic silica nano-magnetic beads. The magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface. By utilizing the superparamagnetism of the nanometer magnetic microspheres coated by the silicon dioxide, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate and the like) and an external magnetic field, DNA and RNA can be separated from samples such as blood, animal tissues, food, pathogenic microorganisms and the like, and the magnetic microsphere can be applied to multiple fields such as clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, molecular biology research and the like.
The nucleic acid amplification detection technology is a process of providing in vitro amplification conditions for nucleic acid fragments, enabling the nucleic acid fragments to be amplified in an exponential manner in a large quantity, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and obtaining a nucleic acid amplification result by processing and analyzing the fluorescent signal.
The steps of cracking, cleaning, eluting, amplifying and the like are included in the process of nucleic acid extraction and amplification detection, the current mainstream technology must be carried out step by step in a standard laboratory, the whole process is easy to pollute the sample nucleic acid, the operation difficulty is high, the requirement on operators is high, and the popularization of the nucleic acid detection technology is not facilitated.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides the nucleic acid extraction amplification detection test tube, which greatly simplifies the operation difficulty from nucleic acid extraction to amplification, improves the detection efficiency, effectively solves the problem that a sample is easy to be polluted, and improves the detection accuracy.
The technical scheme of the invention is as follows: a nucleic acid extraction amplification detection tube, comprising:
the bottom of the pyrolysis liquid pipe is provided with an isolation cavity, a cleaning bin and a reaction pipe;
the isolation cavity, the cleaning bin and the reaction tube are horizontally arranged into a circular or annular shape;
the front end of the isolation cavity is communicated with the bottom of the pyrolysis liquid pipe, and the rear end of the isolation cavity is communicated with the front end of the cleaning bin;
the rear end of the cleaning bin is communicated with the side wall of the reaction tube;
the reaction tube and the cracking liquid tube are mutually separated.
Preferably, the top of the lysis tube is provided with an opening for loading a sample.
Preferably, a sealing cover is arranged at the top of the lysis liquid tube for sealing the lysis liquid tube.
Preferably, the reaction tube is made of transparent material for detecting fluorescence signals.
Preferably, the top of the isolation cavity is provided with an isolation cavity injection hole for injecting a hydrophobic substance.
Preferably, a cleaning bin injection pipe is arranged at the top of the cleaning bin and used for injecting cleaning liquid.
Preferably, a cleaning bin exhaust pipe is arranged at the top of the cleaning bin and used for exhausting air in the bin.
Preferably, the top of the reaction tube is provided with a reaction tube injection hole for injecting the reaction liquid.
Compared with the prior art, the gain effect of the invention is as follows: the detection test tube of the invention combines multiple steps of nucleic acid extraction, cleaning, elution and amplification reaction, and the whole process is carried out in the closed test tube, thereby effectively avoiding sample pollution. The invention can realize the automation of the processes of extracting, cleaning, eluting and amplifying nucleic acid, and greatly simplifies the difficulty of experimental operation.
Drawings
Fig. 1 is a perspective half sectional view of the present invention.
Fig. 2 is a side view of the present invention.
Fig. 3 is a sectional view taken along the line a-a in fig. 2.
The labels in the figures are: 1-a pyrolysis liquid pipe, 2-an isolation cavity, 3-a cleaning bin injection pipe, 4-a cleaning bin exhaust pipe, 5-a cleaning bin, 6-a reaction pipe injection hole, 7-a reaction pipe, 8-an isolation cavity injection hole and 9-a sealing cover.
Detailed Description
The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
Examples are given. A nucleic acid extraction amplification detection tube, as shown in FIG. 1, comprises: the device comprises a pyrolysis liquid pipe 1, wherein the bottom of the pyrolysis liquid pipe 1 is provided with an isolation cavity 2, a cleaning bin 5 and a reaction pipe 7; as shown in fig. 3, the isolation chamber 2, the cleaning bin 5 and the reaction tube 7 are horizontally arranged into a circle; the front end of the isolation cavity 2 is communicated with the bottom of the pyrolysis liquid pipe 1, and the rear end of the isolation cavity 2 is communicated with the front end of the cleaning bin 5; the rear end of the cleaning bin 5 is communicated with the side wall of the reaction tube 7; the reaction tube 7 and the cracking liquid tube 1 are mutually isolated.
The top of the lysis tube 1 as shown in fig. 1 is provided with an opening for the sample to be loaded. The top of the pyrolysis liquid pipe 1 is provided with a sealing cover 9 for sealing the pyrolysis liquid pipe 1. The reaction tube 7 is made of transparent material and is used for detecting a fluorescence signal. The top of the isolation cavity 2 is provided with an isolation cavity injection hole 8 for injecting a hydrophobic substance. The top of the cleaning bin 5 is provided with a cleaning bin injection pipe 3 for injecting cleaning liquid. The top of the cleaning bin 5 is provided with a cleaning bin exhaust pipe 4 for exhausting air in the bin. The top of the reaction tube 7 is provided with a reaction tube injection hole 6 for injecting the reaction liquid.
The specific application method of the invention is as follows:
and (2) sequentially injecting the reaction liquid, the hot-melt paraffin and the biological reagent into a reaction tube 7 through a reaction tube injection hole 6, isolating the reaction liquid and the biological reagent through the paraffin, and injecting silicone oil into the injection hole 6 for isolating the reaction liquid and the lysate. And injecting silicon oil into the isolation cavity 2 through the isolation cavity injection hole 8 to isolate the lysate from the cleaning solution. Injecting cleaning fluid into a cleaning bin 5 through a cleaning bin injection pipe 3, discharging air in the cleaning bin 5 through a cleaning bin exhaust pipe 4, and injecting silicone oil into the cleaning bin injection pipe 3 and the cleaning bin exhaust pipe 4 respectively for isolating the lysate and the cleaning fluid. Finally, the lysate is filled into the lysate tube 1 and covered with the sealing cover 9.
The detection principle of nucleic acid extraction and amplification: loading a detection sample into a lysis liquid tube 1, covering a sealing cover 9, uniformly mixing and lysing by slight shaking, releasing nucleic acid, and allowing nanometer magnetic beads to adsorb the nucleic acid to complete nucleic acid extraction; then, the external magnet drives the nano magnetic beads to carry nucleic acid to pass through silicone oil and enter cleaning liquid for cleaning, paraffin is melted in a temperature control mode to release a biological reagent, then the external magnet continues to drive the nano magnetic beads to carry nucleic acid to pass through silicone oil and enter reaction liquid, so that nucleic acid is eluted, the biological reagent is driven to enter the reaction liquid, nucleic acid amplification is carried out through temperature control on the reaction tube, finally, the nucleic acid amplification process is detected through external optical equipment, and therefore multiple steps of nucleic acid extraction, cleaning, elution and amplification reaction are achieved in the same test tube.
It is to be understood that: although the present invention has been described in detail, the present invention is only for the purpose of illustration and not limitation, and any invention that does not depart from the spirit of the present invention falls within the scope of the present invention.
Claims (8)
1. A nucleic acid extraction amplification detection tube, comprising:
the bottom of the pyrolysis liquid pipe is provided with an isolation cavity, a cleaning bin and a reaction pipe;
the isolation cavity, the cleaning bin and the reaction tube are horizontally arranged into a circular or annular shape;
the front end of the isolation cavity is communicated with the bottom of the pyrolysis liquid pipe, and the rear end of the isolation cavity is communicated with the front end of the cleaning bin;
the rear end of the cleaning bin is communicated with the side wall of the reaction tube;
the reaction tube and the cracking liquid tube are mutually separated.
2. The nucleic acid extraction amplification detection tube of claim 1, wherein: the top of the lysis tube is provided with an opening for loading a sample.
3. The nucleic acid extraction amplification detection tube of claim 1, wherein: the top of schizolysis liquid pipe is provided with sealed lid and is used for sealed schizolysis liquid pipe.
4. The nucleic acid extraction amplification detection tube of claim 1, wherein: the reaction tube is made of transparent material and is used for detecting a fluorescence signal.
5. The nucleic acid extraction amplification detection tube of claim 1, wherein: and an isolation cavity injection hole for injecting a hydrophobic substance is formed in the top of the isolation cavity.
6. The nucleic acid extraction amplification detection tube of claim 1, wherein: and a cleaning bin injection pipe is arranged at the top of the cleaning bin and used for injecting cleaning liquid.
7. The nucleic acid extraction amplification detection tube of claim 1, wherein: the top of the cleaning bin is provided with a cleaning bin exhaust pipe for exhausting air in the cleaning bin.
8. The nucleic acid extraction amplification detection tube of claim 1, wherein: the top of the reaction tube is provided with a reaction tube injection hole for injecting reaction liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120371467.2U CN214496301U (en) | 2021-02-17 | 2021-02-17 | Nucleic acid extraction amplification detection test tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120371467.2U CN214496301U (en) | 2021-02-17 | 2021-02-17 | Nucleic acid extraction amplification detection test tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214496301U true CN214496301U (en) | 2021-10-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120371467.2U Active CN214496301U (en) | 2021-02-17 | 2021-02-17 | Nucleic acid extraction amplification detection test tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214496301U (en) |
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2021
- 2021-02-17 CN CN202120371467.2U patent/CN214496301U/en active Active
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