CN1109502C - Plant regulation disease-resistant bactericide (0.05% nucleotide water agent) and preparation method thereof - Google Patents
Plant regulation disease-resistant bactericide (0.05% nucleotide water agent) and preparation method thereof Download PDFInfo
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- CN1109502C CN1109502C CN 01115369 CN01115369A CN1109502C CN 1109502 C CN1109502 C CN 1109502C CN 01115369 CN01115369 CN 01115369 CN 01115369 A CN01115369 A CN 01115369A CN 1109502 C CN1109502 C CN 1109502C
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- 239000002773 nucleotide Substances 0.000 title claims abstract description 38
- 125000003729 nucleotide group Chemical group 0.000 title claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 7
- 201000010099 disease Diseases 0.000 title claims description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims description 15
- 230000000844 anti-bacterial effect Effects 0.000 title claims description 14
- 239000003899 bactericide agent Substances 0.000 title description 11
- 238000002360 preparation method Methods 0.000 title description 9
- 239000003795 chemical substances by application Substances 0.000 title description 5
- 241000361919 Metaphire sieboldi Species 0.000 claims abstract description 15
- 150000001413 amino acids Chemical class 0.000 claims abstract description 14
- 230000001105 regulatory effect Effects 0.000 claims abstract description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 10
- 239000002207 metabolite Substances 0.000 claims abstract description 8
- 230000008635 plant growth Effects 0.000 claims abstract description 6
- 239000013522 chelant Substances 0.000 claims abstract description 4
- 239000000047 product Substances 0.000 claims description 23
- 239000000203 mixture Substances 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 12
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 9
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 9
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 9
- 239000002509 fulvic acid Substances 0.000 claims description 9
- 229940095100 fulvic acid Drugs 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 8
- 239000002002 slurry Substances 0.000 claims description 8
- 239000011573 trace mineral Substances 0.000 claims description 8
- 235000013619 trace mineral Nutrition 0.000 claims description 8
- 229910052802 copper Inorganic materials 0.000 claims description 6
- 239000010949 copper Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000011701 zinc Substances 0.000 claims description 6
- 229910052725 zinc Inorganic materials 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 5
- 229910052796 boron Inorganic materials 0.000 claims description 5
- 239000010941 cobalt Substances 0.000 claims description 5
- 229910017052 cobalt Inorganic materials 0.000 claims description 5
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 5
- 238000002386 leaching Methods 0.000 claims description 5
- 229910052750 molybdenum Inorganic materials 0.000 claims description 5
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 4
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 4
- 239000012043 crude product Substances 0.000 claims description 4
- 239000011733 molybdenum Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims description 2
- 238000007670 refining Methods 0.000 claims description 2
- 230000009920 chelation Effects 0.000 claims 2
- 238000009413 insulation Methods 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 235000013405 beer Nutrition 0.000 abstract 1
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 14
- 229920002477 rna polymer Polymers 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000003641 microbiacidal effect Effects 0.000 description 5
- 229940124561 microbicide Drugs 0.000 description 5
- 239000002855 microbicide agent Substances 0.000 description 5
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 4
- 229950006790 adenosine phosphate Drugs 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
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- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 230000000853 biopesticidal effect Effects 0.000 description 3
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- 229920002521 macromolecule Polymers 0.000 description 3
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- 210000004681 ovum Anatomy 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 239000003864 humus Substances 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 241000223602 Alternaria alternata Species 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000969780 Eleotris picta Species 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 241000233679 Peronosporaceae Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000013066 combination product Substances 0.000 description 1
- 229940127555 combination product Drugs 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- -1 inorganic acid organic acid Chemical class 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
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- 239000006228 supernatant Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A composite for regulating and killing bacteria of plant growth is prepared from nucleotide, xanthohumic acid, amino acid, the chelate of oxygen element and water, and features that the content of nucleotide is greater than 0.01wt% (total weight), the xanthohumic acid amino acid and part of nucleotide are extracted from earthworm metabolite, the rest part of nucleotide is extracted from waste yeast mud of beer, and the cell wall breaking is performed in said extracting step.
Description
Invention field
The present invention relates to a kind of plant growth regulating disease-resistant microbicide compositions of holding concurrently, wherein nucleotide content is more than or equal to 0.05wt% (aqua), also can remember as plant and regulate disease-resistant bactericide (0.05% aqua), also relates to its preparation method.
Technical background
In the plant growing process, generally use chemical pesticide and carry out the disease-resistant insect pest that removes, and use chemical fertilizer more.Maximum defective is that chemical analysis makes soil compaction, destroys the granular structure of soil; Chemical pesticide cost height, and have high toxic and side effect, high residue, pollution of ecological environment forms public hazards.Therefore, preparing and use green bactericide and green plant growth regulator is trend of the times.
CN1141120A discloses a kind of bactericidal yield-increase agent and preparation method thereof, wherein contains micro-water liquid cultivating earthworm with cow dung sprinkling, again biologic product is extracted in earthworm processing, and this bactericide mainly is to extract amino acid.
CN 1091243A discloses a kind of bactericide, and is wherein formulated with trace element by bridging agent (wormcast) hydrolyzed extract, has volume increase diseases prevention bactericidal effect, and this product extract is mainly humus.
From animal viscera, produce the method for nucleotide in addition, its complex process, the production cost height is unsuitable for wide popularization and application.
In the prior art, do not have a kind of nucleotide content to be higher than the bactericide, well-known of 0.01wt% (weight), nucleotide content is higher than 0.05wt% could effectively carry out wherein growth regulating and sterilization.Nucleotide content does not pass through breaking-wall cell when why not high reason is from earthworm effective component extracting in above-mentioned two pieces of patents, is difficult to extract nucleotide.
In sum, at present relevant plant is regulated the preparation of disease-resistant bactericide (nucleotide content is the aqua of 0.05wt%) and application and yet there are no the file write up or disclose.
Summary of the invention
An object of the present invention is to provide a kind of efficient, low toxicity, non-harmful biopesticide preparation compositions, regulate the disease-resistant bactericide of holding concurrently as plant, thereby according to nuisanceless, pollution-free, environmental protection agricultural industry direction, for improving the health of people level, be the eternal lasting matter of fundamental importance of benefiting the nation and the people, for benefiting the whole mankind, stepped a step.
Another object of the present invention provides a kind of preparation method of plant growth regulating bactericide.
The object of the present invention is achieved like this: a kind of plant growth regulating microbicide compositions of holding concurrently comprises nucleotide, fulvic acid, amino acid, itself and micro-chelate and water, wherein nucleotide content is preferably greater than 0.05wt% greater than 0.01wt% (gross weight).
Fulvic acid in the present composition, amino acid and part nucleotide extract from the earthworm metabolite, and part nucleotide extracts from used yeast slurry in addition.
The preparation method of the present composition comprises extraction step, regulate the nucleotide content step, with the chelated microelement step, wherein extraction step comprises and extracts nucleotide, fulvic acid and amino acid whose step and extract the step of nucleotide from beer yeast slurry from the earthworm metabolite, all passes through breaking-wall cell in the described extraction step.
Detailed Description Of The Invention
Further narrate the present invention below in conjunction with preferred embodiment and embodiment.
The mechanism of action of plant growth regulating microbicide compositions of the present invention is: based on the nucleotide aqua, act on plant cell and virus from molecular level, disturb the synthetic and metabolism of its albumen, make its sex change and death, simultaneously again with plant body cell membrane on receptors bind, import its information of carrying into cell, activate ring type adenosine phosphate (CAMP) earlier, thereby further activate the protease system that depends on the ring type adenosine phosphate again. promote the synthetic and metabolism of its albumen, reach short long, nutrition, sterilization, volume increase, improve quality, realize the effect of " medicine is fertile imitates altogether ".In addition, consider comprehensive utilization wastes of crops (straw, cow dung etc.) and industrial waste (vinasse) etc., through fermenting as the earthworm biological medium, in the metabolite (containing its ovum, residual corpse) of earthworm biological transformation, extract hydrolysis nucleotide (broken wall), fulvic acid, amino acid again, as first head product.With the used yeast slurry is raw material, adopts modern biological project downstream technology, through breaking-wall cell, extracts RNA (ribonucleic acid), and RNA is purified again, be degraded to nucleotide, as second head product, uses it for the nucleotide content that replenishes or adjust finished product.According to up-to-date bioinorganic chemistry theory, with the nucleotide of biologically active, amino acid, and trace copper, cobalt, boron, molybdenum, the zinc isochela of organic macromolecule humus and the biologically active organic macromolecule chelate that synthesizes biologically active be final products.
In one embodiment of the invention, plant to regulate the preparation method of disease-resistant microbicide compositions as follows:
1. cow dung, vinasse, straw, saw are not waited and mix the water spray stacking, and in 20-30 ℃ of heat-preservation fermentation about 15 days, as earthworm medium cultivating earthworm, collect earthworm metabolite (containing ovum, residual corpse) and make raw material, press raw material: PH9 buck=1: 7-2: 10 liquid-solid ratioes drop into retort and extracted 1-5 hour in 50-90 ℃ of broken wall, are incubated at 40-50 ℃ of acidolysis 0.5-2 hour natural subsidence 1-3 days then, the leaching clear liquid is crossed in siphon, makes first head product.Then, measure nucleotide, humic acid, amino acid content with known arbitrary conventional method.
2. make raw material with used yeast slurry, keep 10 ° of following temperature with about 1%NaOH aqueous solution, broken wall extracted 1-4 hour, neutralization staticly settled l-3 days then, cross the leaching clear liquid, acidifying is PH2-3 again, staticly settles 0.5-2 days, get precipitate the RNA crude product, the RNA crude product is transferred about PH5, and hot to 80 ℃ of refining and edulcoration albumen, and acidifying is PH2-3 again, staticly settle the RNA elaboration, then in 1.2% buck, keep about 40 ℃, degraded about 1 day, getting nucleic acid is second head product, reaches as a supplement to adjust agent usefulness.
3. according to measurement result and according to customer requirements, add in first head product, and acidifying with the second head product adjustment, under normal temperature, add copper, cobalt, zinc, molybdenum,
Trace elements such as A, addition mainly determine according to customer requirements, certainly with composition in also relevant with trace element chelated nucleotide, humic acid, amino acid content.About the generally about 1wt% of trace element total amount, obtain finished product behind the chelating.
4. after finished product is tested, the can warehouse-in.
In said process, to buck neither one restriction, hydroxide, carbonate, bicarbonate, ammonia or the like all can, as long as molten water.For example sodium hydroxide, potassium or ammonium, sodium carbonate, potassium or ammonium, or its mixture.Wherein preferred sodium hydroxide or ammonium hydroxide.Acid to acidolysis or acidifying use does not have strict restriction equally, and the inorganic acid organic acid all can.Hydrochloric acid for example, carbonic acid, nitric acid, phosphoric acid, sulfuric acid, acetate, formic acid, propionic acid, oxalic acid, citric acid or the like, wherein preferred hydrochloric acid, phosphoric acid, acetate or citric acid.
Plant of the present invention is regulated disease-resistant microbicide compositions and has following effect:
Its mechanism of action is based on the nucleotide aqua, from molecular level, act on plant cell and virus, disturb the synthetic and metabolism of its albumen, make its sex change and death, combine with the cell-membrane receptor of plant body again simultaneously, import its information of carrying into cell, activate ring type adenosine phosphate (CAMP) earlier, further activate again and depend on the protease system of ring type adenosine phosphate, thereby promote the synthetic and metabolism of its albumen, reach short long, nutrition, sterilization, volume increase and improve quality realize the fertile effect of imitating altogether of medicine.
This product combines the organic macromolecule of biologically active with the necessary various trace elements of plant (Cu, Co, B, Mo, Zn etc.), to improve its slow release and bioavilability.
The present composition is the physical reaction and the combination product of nucleotide/fulvic acid/active ingredients such as trace element, has disease-resistant, nutrition dual-use function; Said composition all has fancy effect to the fusarium wilt of cotton, verticillium wilt, fruit tree putrefaction disease, anthracnose, ring spot, vegetables downy mildew, Alternaria alternate, wheat sharp eyespot, rust, powdery mildew etc., cure rate reaches 91%, efficiently reach 98.7%, 5-20% can be increased production, melon and fruit, tomato sugar part increase 2-5% can be made.
Product of the present invention is efficient, wide spectrum, and nuisanceless novel biopesticide aqua should be according to Different Crop and vegetative period thereof, different regions, dilution is rationally rationally used (seed soaking, irritate root or spray).These all are well known to those skilled in the art, the general about 100g/ mu of consumption.
This product acts on plant cell and virus from molecular level, have short length, growth, volume increase, disease-resistant, sterilization and improve unique effects such as crop quality, be a kind of pure biopesticide, meet the fertile agricultural industry policy of imitating altogether of high-efficiency environment friendly green agriculture and medicine with efficient, wide spectrum, noresidue, non-harmful update.
Specify with embodiment below:
Fig. 1 is that plant is regulated disease-resistant bactericide (0.05% nucleotide aqua) technological process of production figure:
Embodiment one:
Mix, spray water cow dung, sawmilling, vinasse, straw etc. wetting, in about 30 ℃, ferment and feed earthworm after 15 days, collection wormcast, ovum, residual corpse are made raw material, the buck that adds PH9 by 1/7 liquid-solid ratio is gone into retort, heats 70 ℃ and extracts 2 hours, is incubated 50 ℃ of acidolysis 1 hour then, after the natural subsidence 2 days, the siphon supernatant is first head product (nucleotide, fulvic acid, an amino acid)
With the used yeast slurry is that raw material is gone into extractor, adds 1% buck, keeps below 10 ℃, and broken wall extracted 2 hours, precipitate 2 days with PH7 then, cross the leaching clear liquid, acidifying is PH2-3 again, staticly settles 1 day, get precipitate the RNA crude product, RNA is transferred PH5, and heat to 80 ℃, remove foreign protein, acidifying is PH2-3 again, staticly settles 1 day, get the RNA elaboration, in 1% buck, keep about 40 ℃ then, degraded 24 hours, nucleotide second head product, as a supplement, adjust the agent effect.
With waste yeast nucleotide that mud is carried, replenish at random and adjust prepared head product, to guarantee product quality and drug effect fertilizer efficiency.Adjusted head product acidifying is PH2-3, and under normal temperature, add the cobalt of 0.1wt% by weight, the boron of the zinc of 0.2wt% copper, 0.4wt%, the manganese of 0.2wt%, 0.1wt%, making micro-total amount is about 1wt%, stirs chelating and gets final products in about 1 hour.Top embodiment and embodiment limit absolutely not only for explanation.Those skilled in the art can do many improvement not deviating under the present invention spirit scope situation, for example extract amino acid, fulvic acid and nucleotide etc. from other waste materials; When extracting, doing some changes aspect extraction conditions such as the temperature-time according to the different material in the different places of production; Finished product is concentrated back packing or the like, all within the scope of the present invention.
Claims (4)
1. disease-resistant bactericidal composition of plant growth regulating is characterized in that comprising the chelate and the water of fulvic acid, amino acid, nucleotide and they and trace element, and wherein said fulvic acid, amino acid and part nucleotide extract from the earthworm metabolite; And replenish the nucleotide that obtains from the beer yeast slurry extract according to measurement result, make its content greater than 0.05wt% (gross weight); Replenish cobalt, molybdenum, copper, boron and zinc microelement and carry out chelating, make the about 1wt% of total amount (gross weight) of trace element.
2. according to the composition of claim 1, the wherein said step of extracting from the earthworm metabolite comprises collects the earthworm metabolite, according to raw material: the ratio of buck=1: 7 drops into retort, extracted 2 hours in 70 ℃ of broken walls, then 50 ℃ of insulation acidolysis 1 hour, natural subsidence 2 days, the leaching clear liquid is crossed in siphon, as first head product.
3. according to the composition of claim 1, the wherein said step of extracting from beer yeast slurry comprises with the beer yeast slurry being raw material, extracted 2 hours at broken wall below 10 ℃ with the 1%NaOH aqueous solution, being neutralized to neutrality then left standstill 2 days, cross the leaching clear liquid, being acidified to pH=2-3 again left standstill 1 day, gained precipitated rna crude product is regulated pH=5, be heated to 80 ℃ of refining and edulcoration albumen, be acidified to pH=2-3 again and leave standstill, gained RNA elaboration obtains replenishing the nucleotide of usefulness in degraded about 1 day about 40 ℃ in 1.2% buck.
4. according to the composition of claim 1, add cobalt, molybdenum, copper, boron and zinc microelement down in normal temperature when wherein said chelation step is included in acidifying and carry out chelation treatment.
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| CN 01115369 CN1109502C (en) | 2001-04-24 | 2001-04-24 | Plant regulation disease-resistant bactericide (0.05% nucleotide water agent) and preparation method thereof |
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| CN 01115369 CN1109502C (en) | 2001-04-24 | 2001-04-24 | Plant regulation disease-resistant bactericide (0.05% nucleotide water agent) and preparation method thereof |
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| CN1109502C true CN1109502C (en) | 2003-05-28 |
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| CN101830998B (en) * | 2010-05-12 | 2011-11-09 | 黄山市迎客松生物科技有限公司 | Method for extracting ribonucleic acid and cell wall polysaccharides in single cell protein by using one-step method |
| CN102405806A (en) * | 2011-05-23 | 2012-04-11 | 湖北省烟草科研所 | Sustained-release application method of tobacco brown spot prevention and treatment medicament |
| CN102487958A (en) * | 2011-12-06 | 2012-06-13 | 南京同凯兆业生物技术有限责任公司 | Tillering accelerant for plants |
| CN102627488B (en) * | 2012-03-22 | 2014-10-15 | 海南大学应用科技学院（儋州校区） | Flower forcing fertilizer and its preparation method |
| CN112056326A (en) * | 2020-08-31 | 2020-12-11 | 昆山市华明绿化工程有限公司 | Green plant wound healing method |
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