CN110944985B - 用于蛋白酶的成像/检测的化学发光探针 - Google Patents

用于蛋白酶的成像/检测的化学发光探针 Download PDF

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CN110944985B
CN110944985B CN201880047726.7A CN201880047726A CN110944985B CN 110944985 B CN110944985 B CN 110944985B CN 201880047726 A CN201880047726 A CN 201880047726A CN 110944985 B CN110944985 B CN 110944985B
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CN110944985A (zh
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D·莎芭特
M·E·罗斯-康福提
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Ramot at Tel Aviv University Ltd
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    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
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    • C09K11/07—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials having chemically interreactive components, e.g. reactive chemiluminescent compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
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    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06034—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms
    • C07K5/06052—Val-amino acid
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    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96466—Cysteine endopeptidases (3.4.22)

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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CN201880047726.7A 2017-05-24 2018-05-23 用于蛋白酶的成像/检测的化学发光探针 Active CN110944985B (zh)

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US201762510370P 2017-05-24 2017-05-24
US62/510,370 2017-05-24
US201762557881P 2017-09-13 2017-09-13
US62/557,881 2017-09-13
PCT/IL2018/050557 WO2018216012A1 (en) 2017-05-24 2018-05-23 Chemiluminescent probes for imaging/detection of proteases

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CN110944985B true CN110944985B (zh) 2022-12-13

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EP (1) EP3630742B1 (enExample)
JP (2) JP2020520981A (enExample)
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CN112204020B (zh) * 2018-05-25 2023-11-14 尼米斯技术公司 二氧杂环丁烷化合物及其用于检测微生物的用途
WO2021165959A1 (en) * 2020-02-19 2021-08-26 Ramot At Tel-Aviv University Ltd. Chemiluminescence probes for tuberculosis
EP4384515A4 (en) * 2021-08-11 2025-09-03 Univ Nanyang Tech ULTRA-HIGH BRIGHTNESS CHEMILUMINESCENT PROBES FOR DETECTION AND IMAGING
CN114315791B (zh) * 2021-12-22 2023-12-08 东南大学 用于实现手术导航和微小转移瘤成像的小分子化学发光探针及其制备方法和用途
CN114380786B (zh) * 2021-12-23 2023-03-24 苏州大学 基于氨基肽酶激活型化学发光探针及其在恶性肿瘤的活体检测与手术导航方面的应用
CN114716407A (zh) * 2022-03-16 2022-07-08 大连理工大学 一种检测泛酰巯基乙胺酶活性的化学发光探针、制备方法及其生物应用
CN115819416B (zh) * 2022-11-29 2024-06-25 遵义医科大学 一种用于检测和区分的多模态化学发光分子及其应用
WO2024147125A1 (en) * 2023-01-04 2024-07-11 Ramot At Tel-Aviv University Ltd. Cyclobutyl-substituted phenoxy-dioxetanes and uses thereof

Citations (4)

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CN1422253A (zh) * 2000-03-29 2003-06-04 沃泰克斯药物股份有限公司 氨基甲酸酯天冬氨酸特异性半胱氨酸蛋白酶抑制剂及其用途
CN1568307A (zh) * 2000-10-06 2005-01-19 三维药物公司 作为蛋白酶抑制剂的氨基吡啶基－,氨基胍基－和烷氧基胍基－取代苯基乙酰胺
CN101772577A (zh) * 2007-08-03 2010-07-07 塞诺菲-安万特股份有限公司 半胱天冬蛋白酶成像探针
CN106470977A (zh) * 2014-06-26 2017-03-01 里昂高等师范学院 水溶性可活化分子探针、其合成中间体及相关检测方法

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EP3630742B1 (en) 2023-08-16
US20200277647A1 (en) 2020-09-03
ES2963606T3 (es) 2024-04-01
EP3630742A4 (en) 2021-03-03
US12173350B2 (en) 2024-12-24
PL3630742T3 (pl) 2024-04-08
EP3630742A1 (en) 2020-04-08
JP7553615B2 (ja) 2024-09-18
EP3630742C0 (en) 2023-08-16
JP2023038235A (ja) 2023-03-16
WO2018216012A1 (en) 2018-11-29
CN110944985A (zh) 2020-03-31
JP2020520981A (ja) 2020-07-16

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