CN110934139A - Sterilization composition containing physcion and terpene alcohol and application thereof - Google Patents
Sterilization composition containing physcion and terpene alcohol and application thereof Download PDFInfo
- Publication number
- CN110934139A CN110934139A CN201911175659.XA CN201911175659A CN110934139A CN 110934139 A CN110934139 A CN 110934139A CN 201911175659 A CN201911175659 A CN 201911175659A CN 110934139 A CN110934139 A CN 110934139A
- Authority
- CN
- China
- Prior art keywords
- physcion
- terpene alcohol
- composition
- active ingredients
- alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a sterilization composition containing physcion and terpene alcohol and application thereof, wherein the active ingredients in the sterilization composition are physcion and terpene alcohol, and the weight ratio of the physcion to the terpene alcohol is (0.5-5): 5-25. In the bactericidal composition, the physcion and the terpene alcohol are both biological pesticides, so the bactericidal composition has small environmental pollution and is safe; emodin monomethyl ether and terpene alcohol in a weight ratio of 0.5-5: 5-25, has good bactericidal effect, shows synergistic effect on the prevention and treatment of various crop diseases in a certain range, can reduce the usage amount of pesticide active ingredients and improve the prevention and treatment effect.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a sterilization composition containing physcion and terpene alcohol and application thereof.
Background
The walnut anthracnose is a worldwide disease, is distributed in each walnut producing area in China, and is one of the main diseases damaging walnuts. The spread of the anthracnose of the walnut in a large area poses serious threats to the growth and normal fruiting of trees. The disease mainly damages fruits, the fruit damage rate of diseased plants is generally 20% -40%, and the serious damage rate reaches more than 80%; the disease not only causes the young walnut to fall off, but also causes the walnut shell to present a plurality of black dots and the kernel to be shriveled. Not only the yield is reduced, but also the commodity value is greatly reduced, and great economic loss is brought to fruit growers.
At present, the China Ministry of agriculture does not register a medicament for preventing and treating the anthracnose of the walnut, and farmers and users urgently need a high-efficiency, low-toxicity and safe medicament for solving the practical planting production problem.
The physcion has a chemical name of 1, 8-dihydroxy-3-methoxy-6-methylanthraquinone, and is obtained by extracting root of Rheum palmatum L. The physcion is a high-activity botanical fungicide, is prepared by taking a natural plant rhubarb as a raw material, extracting active ingredients of the rhubarb through a core, and then processing the rhubarb, and has good prevention and treatment effects on powdery mildew, downy mildew, gray mold, anthracnose and the like. Low toxicity to human and livestock, environmental protection, and is especially suitable for the production of green and organic vegetables. Terpene alcohol is a botanical fungicide extracted from plants, and the action mechanism is as follows: affecting the permeation and barrier effect of the biological cell membrane structure and destroying the cell membrane and the cell wall. Meanwhile, the composition has the effects of prevention and treatment, and has a bactericidal effect in different stages of plant diseases, such as a spore germination stage, so that spore germination can be effectively inhibited, and the disease diffusion is prevented; the hypha growth and diffusion stage can inhibit the growth and diffusion of (living and in vitro) hyphae; the stage of sporangium growth inhibits the formation of fungal spores, thereby preventing it from infecting new plant tissues.
A binary compound patent of physcion and terpene alcohol for preventing and removing walnut diseases is not reported at present. The two are compounded according to a certain proportion, and the composition has outstanding effects on walnut anthracnose and the like.
Disclosure of Invention
The invention aims to provide a sterilization composition containing physcion and terpene alcohol and application thereof, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the sterilization composition containing physcion and terpene alcohol comprises the active components of physcion and terpene alcohol, wherein the weight ratio of the physcion to the terpene alcohol is 0.5-5: 5-25.
As a further scheme of the invention: the weight ratio of the physcion to the terpene alcohol is 2: 15.
as a further scheme of the invention: the mass of the active ingredients accounts for 2-75% of the total mass of the sterilization composition.
As a further scheme of the invention: the dosage form of the bactericidal composition is dispersible oil suspending agent, suspending emulsion, missible oil, microemulsion, emulsion in water, wettable powder or water dispersible granule.
As a further scheme of the invention: the bactericidal composition is used for preventing and treating anthracnose of the walnut.
Further, the fungicidal composition of the present invention may be used in combination with other active ingredients, including, but not limited to, one or more of fungicides, insecticides, plant growth regulators, herbicides, safeners, synergists, fertilizers, and the like.
The bactericidal composition can be directly provided in the form of a mixed preparation, namely all active ingredients in the composition are uniformly mixed, or the active ingredients in the composition can be provided in the form of a single preparation which is directly mixed in a barrel or a tank before use and then diluted to a required concentration, or the active ingredients can be respectively diluted by the single preparation and applied to plant diseases or the living environment of the plant diseases.
Compared with the prior art, the bactericidal composition has the beneficial effects that:
1. in the bactericidal composition, the physcion and the terpene alcohol are both biological pesticides, so the bactericidal composition has small environmental pollution and is safe;
2. in the bactericidal composition, the weight ratio of the physcion to the terpene alcohol is 0.5-5: 5-25, the bactericidal effect is good, the synergistic effect is shown in the prevention and control of various crop diseases in a certain range, the usage amount of pesticide active ingredients can be reduced, and the prevention and control effect is improved;
3. in the bactericidal composition, the physcion and the terpene alcohol are bactericides with different action mechanisms, and the combined use of the physcion and the terpene alcohol is favorable for enhancing the action site of the active ingredient of the pesticide, so that the generation of the drug resistance of pathogenic bacteria can be overcome and/or delayed, the service life of the active ingredient of the pesticide is prolonged, and the use cost is reduced;
4. the bactericidal composition has high control efficiency, low toxicity, environmental protection and safety to crops.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
First, preparation example:
example 1
Preparation of 15% physcion terpene alcohol WP (1.5+13.5)
1.5kg of physcion, 13.5kg of terpene alcohol, 2kg of dispersant naphthalenesulfonate formaldehyde condensate NNO, 2.1kg of wetting agent lauryl sodium sulfate K12, 3kg of white carbon black and light calcium carbonate are taken to make up to 100 kg.
The wettable powder is obtained by fully mixing, airflow crushing and mixing the raw materials.
Example 2
Preparation of 17% physcion terpene alcohol SC (2+15)
2kg of physcion, 15kg of terpene alcohol, 6kg of sodium lignosulfonate (dispersing agent), 3.1kg of fatty alcohol polyoxyethylene ether (wetting agent), 1.3kg of xanthan gum (thickening agent), 0.9kg of sodium benzoate, (preservative), 1.3kg of polyoxyethylene polyoxypropylene amine ether (defoaming agent), 4.2kg of ethylene glycol (antifreezing agent) and carrier water are taken for complement. The suspending agent is obtained by fully mixing, blending, ball milling, crushing, adjusting and packaging.
Example 3
Preparation of 18% physcion-terpene alcohol WG (3+15)
3kg of physcion, 15kg of terpene alcohol, 5kg of dispersant naphthalene sulfonate formaldehyde condensate (D-425), 3.5kg of wetting agent alkyl naphthalene sulfonate and anion wetting agent mixture (EFW), 3.1kg of disintegrant ammonium sulfate, 2.6kg of binder polyvinyl alcohol (PVA) and 32kg of carrier kaolin are taken. The water dispersible granules are obtained by fully mixing, airflow crushing, mixing, granulating, drying and screening.
Example 4
Preparation of 17% physcion terpene alcohol OD (2+15)
2kg of physcion, 15kg of terpene alcohol, 10.6kg of dispersant-block polyether nonionic surfactant (Pluronic125), 1.6kg of thickening agent-magnesium aluminum silicate and 62kg of carrier methyl oleate are taken. The dispersible oil suspending agent is obtained by fully mixing, blending, ball milling, crushing, adjusting and packaging.
Example 5
Preparation of 10% physcion terpene alcohol EC (5+5)
Taking 5kg of physcion, 5kg of terpene alcohol, 15kg of alkylphenol polyoxyethylene, 2kg of calcium dodecyl benzene sulfonate and 55kg of carrier. The missible oil is obtained by fully mixing, blending, ball milling, crushing, adjusting and packaging.
Example 6
Preparation of 20.5% physcion terpene alcohol WP (0.5+ 25):
taking 0.5kg of physcion 20.5kg of terpene alcohol, 15kg of alkylphenol polyoxyethylene, 3kg of dispersing agent naphthalenesulfonate formaldehyde condensate NNO, 2.2kg of wetting agent sodium dodecyl sulfate K12, 3kg of white carbon black and light calcium carbonate to make up to 100 kg. The wettable powder is obtained by fully mixing, airflow crushing and mixing.
Second, determination of Activity
Respectively detecting the bacteriostatic activity of the single agent and the compound agent of the physcion and the terpene alcohol on the colletotrichum gloeosporioides:
(1) test strains: pathogenic bacteria of walnut anthracnose
(2) Reagent to be tested: 97% of physcion original drug, terpene alcohol original drug and the company of Limited liability of agriculture of Anhui Fengle.
Dissolving physcion original drug, terpene alcohol original drug and 5 physcion and terpene alcohol mixed preparations with acetone respectively, diluting with 0.1% Tween 80 water solution, setting 5 dosages respectively, setting blank control, and repeating each test treatment for 4 times. Specifically, the physcion is diluted to be prepared into five series concentrations of 0.125, 0.25, 0.5, 1 and 2 ml/L; terpene alcohol is diluted to five series concentrations of 0.125, 0.25, 0.5, 1 and 2 ml/L; then 5 preparations (physcion: terpene alcohol is 0.5: 25, 1.5: 20, 2:15, 3.5: 10 and 5: 5) are mixed and diluted to five series concentrations of 0.125, 0.25, 0.5, 1 and 2ml/L for use.
(3) The test method comprises the following steps: according to the experimental criteria NY/T1156.2-2006 for biological determination of pesticide, the indoor toxicity of single agent and mixed agents with different proportions to pathogenic bacteria is determined by a hypha growth rate method.
The prepared liquid medicine is absorbed by a sterilizing pipette with 1ml and placed in a sterilizing culture dish, then 9ml of dissolved culture medium is absorbed by a 10ml pipette and placed in the dish to be mixed uniformly immediately, and 3 times of repeated setting are carried out for each concentration, and the liquid medicine is not added as a reference. The cultured test germs are punched by a stainless steel puncher with the diameter of 5cm, picked by an inoculating loop, placed in the center of a cooled medicine mixing culture medium, and cultured for several days at 28 ℃.
(4) And (3) data analysis: adopting a DPS13.0 professional version data processing system to calculate a regression equation of the single medicament and the compound agents with different proportions on the inhibition of the growth of the hyphae, EC50 and 95 percent confidence limit thereof.
The colony diameters were measured periodically based on the control hyphae growing up to the edge of the dish, and the average colony diameter at each concentration was calculated and expressed as the linear length to determine the percentage inhibition. The regression line equation was then developed as the log concentration-percent inhibition probability value. Calculating EC according to the equation50Value, comparison of virulence size, and by EC50The co-toxicity coefficient of each mixture is measured. And comprehensively analyzing and evaluating the observation result according to 4 indexes of virulence index, theoretical virulence index, actual virulence index and cotoxicity coefficient.
Virulence index ═ EC of standard fungicides50EC of value/agent tested50Value) × 100%
Actual virulence index ═ standard killEC of microbial inoculum50EC of value/blend50Value) × 100%
Theoretical virulence index ═ A virulence index x A content (%) in the mixture + B virulence index x B content (%) in the mixture
The cotoxicity coefficient is (actual virulence index/theoretical virulence index) × 100%
The invention uses the Sunweier co-toxicity coefficient method to evaluate the combined action after mixing, the co-toxicity coefficient is the actual toxicity index-theoretical toxicity index, if more than 120, the synergy is realized.
TABLE 1 test treatment and test levels (dosage ml/L)
TABLE 2 prevention of Collybia anthracnose by physcion and terpene alcohol
As can be seen from Table 2, the respective mixed preparation of physcion and terpene alcohol has better inhibition effect on the pathogenic bacteria of the walnut anthracnose, and the inhibition percentage of 2ml/L on the pathogenic bacteria of the walnut anthracnose is more than 88%. The co-toxicity coefficient of each mixture is shown in table 3 by using terpene alcohol as a standard medicament.
Table 3: combined action of physcion and terpene alcohol mixed on colletotrichum gloeosporioides
| Treatment of | Regression equation | Correlation coefficient | LD50 | Actual virulence index | Theoretical virulence index | Co-toxicity coefficient |
| A | Y＝0.7376x+3.6301 | 0.9973 | 1.5186 | 80.3 | / | / |
| B | Y＝0.7097x+3.7670 | 0.9953 | 1.2267 | 100 | / | / |
| C | Y＝0.5147x+4.1472 | 0.9980 | 0.6332 | 193.7 | 99.6 | 194.5 |
| D | Y＝0.4832x+4.1940 | 0.9976 | 0.5589 | 219.4 | 98.6 | 222.5 |
| E | Y＝0.3973x+4.3447 | 0.9978 | 0.3365 | 364.5 | 97.7 | 373.1 |
| F | Y＝0.4300x+4.2330 | 0.9987 | 0.4739 | 258.9 | 94.9 | 272.8 |
| G | Y＝0.4772x+4.2361 | 0.9936 | 0.5071 | 241.9 | 90.3 | 267.8 |
As can be seen from table 3, when physcion and terpene alcohol are mixed, the co-toxicity coefficient of each mixed preparation is greater than 120, and the mixed preparation has a synergistic effect, particularly, physcion and terpene alcohol are 2:15, the co-toxicity coefficient is the maximum and reaches 373.1, and the synergistic effect is obvious.
Third, field drug effect test
A field efficacy test for preventing and treating anthracnose of a walnut was carried out using 17% physcion-terpene alcohol SC (physcion: terpene alcohol: 2:15), 2% terpene alcohol SC and 15% physcion SC prepared in example 2.
The test treatment method comprises the following steps: the test site is located in a test field of a Yunan university farm. The experiment was conducted with 5 treatments, respectively: 17% physcion-terpene alcohol SC (800 times liquid, 1600 times liquid), control agent 20% physcion WDG (800 times liquid), 10% terpene alcohol AS (800 times liquid) and clear water blank control, each treating four repeat cells. The application is carried out for 1 time in the walnut anthracnose, and equal amount of clear water is sprayed in a blank control area. And no other bactericide is sprayed during the test period, and the field management is carried out according to the convention. The disease condition is investigated 20 days after the application, and the investigation method comprises the following steps: visual inspection, investigating the number of diseased leaves and fruits, and calculating the morbidity and the prevention effect.
Incidence (%) × 100 (number of diseased leaves/total investigated leaves) × 100
Disease index [ Σ (number of diseased leaves and fruits at each stage × representative value at each stage)/(total investigated leaf and fruit number × representative value at highest stage) ] × 100
Control effect (%) [ (clear water control disease index-treatment disease index)/clear water control disease index ]. times.100
The test results are shown in Table 4:
TABLE 4 field control of walnut anthracnose with different agent treatments (%)
The experimental result shows that the 17 percent physcion-terpene alcohol suspending agent has the control effect of more than 80 percent, and has obvious synergistic action compared with the single use of one of the active ingredients. The compound bactericide has the advantages of obvious disease prevention effect, environmental friendliness, no influence on next-stubble crops, small environmental pollution, safety to people, livestock, birds and beneficial organisms and contribution to keeping ecological balance. In addition, the bactericide is processed by a physical method, the production process is simple, three wastes are not discharged in the production process, and no adverse effect is caused on the ecological environment. Is a compound bactericide with ideal prevention and treatment effects at present.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A sterilization composition containing physcion and terpene alcohol is characterized in that: the active ingredients of the composition are physcion and terpene alcohol, wherein the weight ratio of the physcion to the terpene alcohol is 0.5-5: 5-25.
2. The germicidal composition of claim 1, wherein: the weight ratio of the physcion to the terpene alcohol is 2: 15.
3. the germicidal composition of claim 1, wherein: the mass of the active ingredients accounts for 2-75% of the total mass of the sterilization composition.
4. The germicidal composition of claim 1, wherein: the dosage form of the bactericidal composition is dispersible oil suspending agent, suspoemulsion, missible oil, microemulsion, emulsion in water, wettable powder or water dispersible granule.
5. Use of a fungicidal composition according to claim 1, characterized in that: the bactericidal composition is used for preventing and treating anthracnose of the walnut.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911175659.XA CN110934139A (en) | 2019-11-26 | 2019-11-26 | Sterilization composition containing physcion and terpene alcohol and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911175659.XA CN110934139A (en) | 2019-11-26 | 2019-11-26 | Sterilization composition containing physcion and terpene alcohol and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110934139A true CN110934139A (en) | 2020-03-31 |
Family
ID=69908982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911175659.XA Pending CN110934139A (en) | 2019-11-26 | 2019-11-26 | Sterilization composition containing physcion and terpene alcohol and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110934139A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1602692A (en) * | 2003-09-29 | 2005-04-06 | 湖北省农业科学院植保土肥研究所 | Emodin monomethyl ether for preventing plant fungus disease |
| CN107006503A (en) * | 2017-04-11 | 2017-08-04 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition containing pyraclostrobin and its application |
| CN107410315A (en) * | 2017-05-31 | 2017-12-01 | 江门市植保有限公司 | Bactericidal composition containing kasugarnycin and terpenol |
| CN107467036A (en) * | 2017-09-03 | 2017-12-15 | 佛山市瑞生通科技有限公司 | Composition pesticide and its application containing prothioconazoles and terpenol |
-
2019
- 2019-11-26 CN CN201911175659.XA patent/CN110934139A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1602692A (en) * | 2003-09-29 | 2005-04-06 | 湖北省农业科学院植保土肥研究所 | Emodin monomethyl ether for preventing plant fungus disease |
| CN107006503A (en) * | 2017-04-11 | 2017-08-04 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition containing pyraclostrobin and its application |
| CN107410315A (en) * | 2017-05-31 | 2017-12-01 | 江门市植保有限公司 | Bactericidal composition containing kasugarnycin and terpenol |
| CN107467036A (en) * | 2017-09-03 | 2017-12-15 | 佛山市瑞生通科技有限公司 | Composition pesticide and its application containing prothioconazoles and terpenol |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101971812A (en) | Eugenol-containing germicidal composition | |
| CN1327770C (en) | Sterilization composition containing carbendazim of multiple antimycin | |
| CN105961406A (en) | Pesticide composition containing oxathiapiprolin and quinoline copper | |
| CN107372556A (en) | A kind of suspension seed-coating agent containing pyraclostrobin, amino-oligosaccharide and Diacloden | |
| CN101658173A (en) | Sterilization composition containing tetraconazole | |
| CN114617127B (en) | Pesticide sterilization composition and application thereof | |
| CN106234389B (en) | A kind of composition pesticide of fluorine-containing thiazole pyrrole ethyl ketone and jamaicin | |
| CN107372528B (en) | Sterilization composition containing prothioconazole and ethylicin and application thereof | |
| CN104886122B (en) | Compound composition containing dufulin and pyrazole amide bactericide and bactericide | |
| CN102150676B (en) | Synergic sterilization composition containing captan | |
| CN110934139A (en) | Sterilization composition containing physcion and terpene alcohol and application thereof | |
| CN101755836B (en) | Bactericidal composite taking fluazinam as main ingredient | |
| CN107333791A (en) | By rosickyite azoles and the bactericide of the preventing and treating rice blast of OIT compounding | |
| CN102771488B (en) | Bactericidal composition containing isothiazolone | |
| CN101455203B (en) | Osthole and carbendazim compound composition and use thereof | |
| CN104823976A (en) | Pesticide composition containing bromothalonil and kasugamycin | |
| CN104798794A (en) | Captan and imazalil compound composition and application thereof | |
| CN114190392B (en) | Sterilization composition containing difenoconazole and ketoconazole and application thereof | |
| CN110934142A (en) | Bactericidal composition containing mandipropamid and indole flusulfamide and application thereof | |
| CN108477199A (en) | A kind of bactericidal composition and application thereof | |
| CN108849923A (en) | A kind of biological composite bactericide for preventing and treating wheat scab | |
| CN110973140A (en) | Sterilization composition containing penflufen and penthiopyrad and application thereof | |
| CN109392932A (en) | A kind of bactericidal composition containing coumoxystrobin and Zhongshengmycin | |
| CN104904730B (en) | Sterilization composition containing ZJ5337 and triazole bactericide | |
| CN108651468B (en) | Pesticide composition containing L-pyroglutamic acid, soluble powder, soluble liquid and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200331 |
|
| WD01 | Invention patent application deemed withdrawn after publication |