CN110934142A

CN110934142A - Bactericidal composition containing mandipropamid and indole flusulfamide and application thereof

Info

Publication number: CN110934142A
Application number: CN201911175660.2A
Authority: CN
Inventors: 金立; 龚国斌; 葛亮亮; 陈克付; 孙永强
Original assignee: Anhui Fengle Agrochemical Co Ltd
Current assignee: Anhui Fengle Agrochemical Co Ltd
Priority date: 2019-11-26
Filing date: 2019-11-26
Publication date: 2020-03-31

Abstract

The invention discloses a bactericidal composition containing mandipropamid and indole flusulfamide, which comprises active ingredients of mandipropamid and indole flusulfamide, wherein the weight ratio of the mandipropamid to the indole flusulfamide is (5-21): 2-14. Also discloses the application of the composition in preventing and treating the garlic epidemic disease. The bactericidal composition provided by the invention expands the action ranges of mandipropamid and indole flusulfamide, reduces the application amount of mandipropamid and indole flusulfamide, and keeps good action at the same time. Secondly, the combination of fluxapyroxad and penthiopyrad still achieves a high degree of plant pathogen control even if the individual compounds become completely ineffective at low application rates. On one hand, the spectrum of the plant pathogens which can be prevented and controlled is expanded, and on the other hand, the use safety is improved.

Description

Bactericidal composition containing mandipropamid and indole flusulfamide and application thereof

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to a bactericidal composition containing mandipropamid and indole flusulfamide and application thereof.

Background

The garlic epidemic disease is mainly harmful to the leaves, pale yellow to yellowish water-soaked spots are generated in the middle of the leaves or on the leaf tips at the beginning of the infection of the leaves, the edges are light green, the disease spots are quickly expanded, half or the whole leaves are withered and drooped in the near future, the disease spots are decayed when the humidity is high, and sparse grey white mildew, namely pathogen sporangium and sporangium, is generated on the disease spots; the infected scape is also rotten in the form of water stain, so that the whole plant dies. In recent years, the disease of continuous cropping garlic is easy to develop in spring, and the disease tends to be aggravated year by year, and becomes one of the most serious diseases harming the normal growth of garlic.

The garlic epidemic disease is mainly prevented in advance, namely, pesticides such as azoxystrobin, mancozeb, fosetyl-aluminum and the like are selected for spraying prevention in the green turning period of garlic. At present, no pesticide for preventing and treating garlic epidemic disease is registered in the Ministry of agriculture in China, and farmers and users urgently need a high-efficiency, low-toxicity and safe pesticide for solving the problem of actual planting production.

The mandipropamid is an amide bactericide, has an action mechanism of inhibiting the biosynthesis of phospholipid, and has a good control effect on most of leaf and fruit diseases caused by oomycetes. Has high activity on spore in germination stage, and can inhibit hypha growth and sporulation. Amisulbrom (Amisulbrom) is a triazole sulfonamide bactericide developed in the last 90 th century of daily-produced chemistry, and has high activity on phytophthora blight and downy mildew caused by oomycetes.

A binary compound patent of mandipropamid and indoxamine for preventing and killing garlic epidemic disease is not reported at present. The two are compounded according to a certain proportion, and the composition has outstanding effects on garlic epidemic diseases and the like.

Disclosure of Invention

The invention aims to provide a bactericidal composition containing mandipropamid and indoxamine and application thereof, and aims to solve the problem of prevention and treatment of garlic epidemic diseases.

In order to achieve the purpose, the invention provides the following technical scheme:

the bactericidal composition containing mandipropamid and indole flusulfamide comprises active ingredients of mandipropamid and indole flusulfamide, wherein the weight ratio of the mandipropamid to the indole flusulfamide is (5-21): 2-14.

Further, the weight ratio of mandipropamid to indole flusulfamide is 13: 8.

further, the sum of the mass of the mandipropamid and the mass of the indoxyl flusulfamide accounts for 5-85% of the total mass of the bactericidal composition.

In a further scheme, the dosage form of the bactericidal composition is dispersible oil suspending agent, suspending emulsion, missible oil, microemulsion, aqueous emulsion, wettable powder or water dispersible granules.

The invention also aims to provide application of the bactericidal composition for preventing and treating the garlic epidemic disease.

Further, the bactericidal composition of the present invention may be used in combination with other active ingredients, including but not limited to one or more of bactericides, insecticides, plant growth regulators, herbicides, safeners, synergists, fertilizers, and the like.

The bactericidal composition can be directly provided in the form of a mixed preparation, namely, all active ingredients in the composition are uniformly mixed, or the active ingredients in the composition can be provided in the form of a single preparation which is directly mixed in a barrel or a tank before use and then diluted to a required concentration, or all the active ingredients in the single preparation can be respectively diluted and independently applied to plant diseases or the living environment thereof.

Compared with the prior art, the bactericidal composition has the beneficial effects that:

the bactericidal composition provided by the invention expands the action ranges of mandipropamid and indole flusulfamide, reduces the application amount of mandipropamid and indole flusulfamide, and keeps good action at the same time. Secondly, the combination of mandipropamid and indosulam still achieves a high degree of plant pathogen control even if the individual compounds become completely ineffective at low application rates. On one hand, the spectrum of the plant pathogens which can be prevented and controlled is expanded, and on the other hand, the use safety is improved.

The mandipropamid and the indole flusulfamide in the bactericidal composition are 5-21: 2-14, has good bactericidal effect, shows synergistic effect on the prevention and the treatment of various crop diseases in a certain range, can reduce the using amount of pesticide active ingredients, and can overcome and/or delay the generation of drug resistance of pathogenic bacteria, thereby prolonging the service life of the pesticide active ingredients, reducing the drug cost and improving the prevention and the treatment effect.

In addition, the bactericidal composition is low in toxicity, environment-friendly, small in environmental pollution and safe to crops.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

First, preparation example:

example 1

Preparation of 19% mandipropamid-indoxyl flusulfamide WP (5+ 14):

5kg of mandipropamid, 14kg of indoxyl flusulfamide, 4kg of dispersant naphthalenesulfonate formaldehyde condensate NNO, 2kg of wetting agent lauryl sodium sulfate K12, 3.5kg of white carbon black and light calcium carbonate are taken to make up to 100 kg. The wettable powder is obtained by fully mixing, airflow crushing and mixing.

Example 2

Preparation of 21% mandipropamid-indoxamine SC (13+ 8):

13kg of mandipropamid, 8kg of indoxyl flusulfamide, 7kg of sodium lignosulfonate (dispersing agent), 3.6kg of fatty alcohol-polyoxyethylene ether (wetting agent), 1.3kg of xanthan gum (thickening agent), 0.9kg of sodium benzoate, (preservative), 1.5kg of polyoxyethylene polyoxypropylene amine ether (defoaming agent), 4.7kg of ethylene glycol (antifreezing agent) and carrier water are taken for complement. The suspending agent is obtained by fully mixing, blending, ball milling, crushing, adjusting and packaging.

Example 3

Preparation of 24% mandipropamid-indoxyl flusulfamide (12+12) SC:

taking 12kg of mandipropamid, 12kg of indoxyl flusulfamide, 11.3kg of sodium lignosulfonate (dispersing agent), 4.2kg of fatty alcohol-polyoxyethylene ether (wetting agent), 0.68kg of xanthan gum (thickening agent), 0.8kg of sodium benzoate, (preservative), 1.4kg of polyoxyethylene polyoxypropylene ether (defoaming agent), 6.1kg of ethylene glycol (antifreezing agent) and the balance of carrier water. The suspending agent is obtained by fully mixing, blending, ball milling, crushing, adjusting and packaging.

Example 4

Preparation of 26% mandipropamid-indole flusulfamide WG (16+10)

16kg of mandipropamid, 10kg of indoxyl flusulfamide, 5.3kg of a dispersant-naphthalenesulfonate formaldehyde condensate (D-425), 5.5kg of a wetting agent-a mixture (EFW) of alkylnaphthalenesulfonate and an anionic wetting agent, 3.2kg of a disintegrant-ammonium sulfate, 2.0kg of a binder-polyvinyl alcohol (PVA) and 35kg of carrier kaolin. The water dispersible granules are obtained through full mixing, airflow crushing, mixing, granulation, drying and screening.

Example 5

Preparation of 17% mandipropamid-indole flusulfamide OD (8+ 9):

8kg of mandipropamid, 9kg of indole flusulfamide, 11kg of dispersant-block polyether nonionic surfactant (Pluronic125), 1.6kg of thickening agent-magnesium aluminum silicate and 61kg of carrier methyl oleate are taken. The dispersible oil suspending agent is obtained by fully mixing, blending, ball milling, crushing, adjusting and packaging.

II, activity determination:

respectively detecting the bacteriostatic activity of the single agent and the compound agent of mandipropamid and indoxyl sulfamide on the garlic phytophthora blight bacteria:

(1) test strains: pathogenic bacteria of garlic blight

(2) Reagent to be tested: 93 percent of mandipropamid technical material, 96 percent of indolophostrobin technical material and Anhui Fengle agro-chemical company with limited responsibility.

Dissolving the technical products of mandipropamid and indole flusulfamide and 5 mixed preparations of mandipropamid and indole flusulfamide in acetone respectively, diluting the dissolved products by using 0.1% Tween 80 aqueous solution, setting 5 dosages respectively, setting blank controls additionally, and repeating each test treatment for 4 times. Specifically, the mandipropamid is diluted to be prepared into five series concentrations of 10, 5, 2.5, 1.25 and 0.625 ml/L; the indoxyl flusulfamide is diluted to be prepared into five series concentrations of 10, 5, 2.5, 1.25 and 0.625 ml/L; then 5 preparations of mandipropamid and indole flusulfamide are mixed (the mandipropamid and the indole flusulfamide are 5: 14, 9: 11, 13:8, 17: 5 and 21: 2 respectively), and are diluted to five series concentrations of 10, 5, 2.5, 1.25 and 0.625ml/L, and are prepared for use.

(3) The test method comprises the following steps: according to the experimental criteria NY/T1156.2-2006 for biological determination of pesticide, the indoor toxicity of single agent and mixed agents with different proportions to pathogenic bacteria is determined by a hypha growth rate method.

The prepared liquid medicine is absorbed by a sterilizing pipette with 1ml and placed in a sterilizing culture dish, then 9ml of dissolved culture medium is absorbed by a 10ml pipette and placed in the dish to be mixed uniformly immediately, and 3 times of repeated setting are carried out for each concentration, and the liquid medicine is not added as a reference. The cultured test germs are punched by a stainless steel puncher with the diameter of 5cm, picked by an inoculating loop, placed in the center of a cooled medicine mixing culture medium, and cultured for several days at 28 ℃.

(4) And (3) data analysis: adopting a DPS13.0 professional version data processing system to calculate a regression equation of the single medicament and the compound agents with different proportions on the inhibition of the growth of the hyphae, EC50 and 95 percent confidence limit thereof.

The colony diameters were measured periodically based on the control hyphae growing up to the edge of the dish, and the average colony diameter at each concentration was calculated and expressed as the linear length to determine the percentage inhibition. Then, a regression line equation is obtained by using the concentration logarithm and the inhibition percentage probability value. Calculating EC according to the equation₅₀Value, comparison of virulence size, and by EC₅₀The co-toxicity coefficient of each mixture is measured. And comprehensively analyzing and evaluating the observation result according to 4 indexes of the virulence index, the theoretical virulence index, the actual virulence index and the cotoxicity coefficient.

Virulence index ═ EC of standard fungicides₅₀EC of value/agent tested₅₀Value) × 100%

Actual virulence index (EC of standard fungicide)₅₀EC of value/blend₅₀Value) × 100%

Theoretical virulence index ═ A virulence index x A content (%) in the mixture + B virulence index x B content (%) in the mixture

The cotoxicity coefficient is (actual virulence index/theoretical virulence index) × 100%

The invention uses the Sunweier co-toxicity coefficient method to evaluate the combined action after mixing, the co-toxicity coefficient is the actual toxicity index-theoretical toxicity index, if more than 120, the synergy is realized.

TABLE 1 test treatment and test levels (dosage mg/L)

TABLE 2 Depression rates of mandipropamid and Tribencarb on Phytophthora allici

As can be seen from Table 2, the mandipropamid and indoxyl flusulfamide mixed preparation has a good inhibition effect on the garlic epidemic disease, and the inhibition percentage of 10ml/L on the garlic epidemic disease is more than 90%. The cotoxicity coefficient of each mixture is measured by taking the indosulfamide as a standard medicament and is shown in table 3.

Table 3: combined action of mandipropamid and indoxyl flusulfamide on garlic phytophthora root rot

As can be seen from table 3, the co-toxicity coefficient of the mandipropamid and the indole flusulfamide is greater than 120, and the synergistic effect is achieved, particularly, the mandipropamid and the indole flusulfamide are 13:8 has the maximum co-toxicity coefficient and has obvious synergistic effect.

Third, field drug effect test

21% of mandipropamid-indole flusulfamide SC (mandipropamid: indole flusulfamide ═ 13:8) prepared in example 2, and the prepared 13% of mandipropamid SC (prepared by self) and 8% of indole flusulfamide SC (prepared by self) are compared by single dose, so that a field efficacy test for preventing and treating garlic blight is carried out.

The test treatment method comprises the following steps: the test site was located in the test field on a farm in Bozhou city, Anhui province. The experiment is provided with 5 treatments, each treatment is carried out on four cells, and the treatment is respectively as follows: spraying 21% mandipropamid-indole flusulfamide SC (active ingredients are 6 g/mu and 12 g/mu), spraying 13% mandipropamid WDG (active ingredients are 12 g/mu) and 8% indole flusulfamide AS (active ingredients are 10 g/mu) AS a contrast agent, and performing clear water blank contrast. The garlic epidemic disease is applied for 1 time, and the same amount of clear water is sprayed in a blank control area. And no other bactericide is sprayed during the test period, and the field management is carried out according to the convention. The disease condition is investigated 20 days after the application, and the investigation method comprises the following steps: and (4) performing visual inspection, investigating the disease area of epidemic diseases, and calculating the disease rate and the prevention and treatment effect.

The disease index and the prevention and treatment effect are respectively calculated by the following formulas:

disease index [ Σ (number of diseased leaves (flowers) at each stage × corresponding disease-level numerical value)/total number of investigated leaves × highest-level representative value ] × 100;

the prevention and treatment effect is [ (control disease index-treatment disease index)/control disease index ] × 100%.

The test result shows that the 16% mandipropamid-indole flusulfamide SC has the best control effect and obvious difference. The pesticide composition containing mandipropamid and the indole flusulfamide has good control effect on the garlic epidemic disease, and the pesticide effect in the field is obviously enhanced compared with that of a single agent; the results are shown in Table 4.

TABLE 4 field control of garlic blight by different agent treatments (%)

The experimental result shows that the 21% mandipropamid-indole flusulfamide suspending agent has the control effect of over 84%, and has obvious synergistic effect compared with the single use of one of the effective components. The compound bactericide has the advantages of obvious disease prevention effect, environmental friendliness, no influence on next-stubble crops, small environmental pollution, safety to people, livestock, birds and beneficial organisms and contribution to keeping ecological balance. In addition, the bactericide is processed by a physical method, the production process is simple, three wastes are not discharged in the production process, and no adverse effect is caused on the ecological environment. Is a compound bactericide with ideal prevention and treatment effects at present.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A bactericidal composition containing mandipropamid and indoxyl flusulfamide is characterized in that: the active ingredients of the compound are mandipropamid and indole flusulfamide, and the weight ratio of the mandipropamid to the indole flusulfamide is 5-21: 2-14.

2. The bactericidal composition according to claim 1, characterized in that the weight ratio of mandipropamid to indole flusulfamide is 13: 8.

3. the garlic bactericidal composition of claim 1, wherein: the sum of the mass of the mandipropamid and the mass of the indoxyl flusulfamide accounts for 5-85% of the total mass of the bactericidal composition.

4. The bactericidal herbicidal composition of claim 1, wherein: the dosage form of the bactericidal composition is dispersible oil suspending agent, suspoemulsion, missible oil, microemulsion, emulsion in water, wettable powder or water dispersible granule.

5. Use of a fungicidal composition according to claim 1, characterized in that: can be used for preventing and treating garlic epidemic disease.