CN110934099B

CN110934099B - Method for inhibiting river crab fighting by DA receptor

Info

Publication number: CN110934099B
Application number: CN201911090383.5A
Authority: CN
Inventors: 杨筱珍; 庞杨洋; 成永旭
Original assignee: Shanghai Ocean University
Current assignee: Shanghai Ocean University
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2021-09-24
Anticipated expiration: 2039-11-08
Also published as: CN110934099A

Abstract

The invention discloses a method for inhibiting river crab fighting by using DA receptors, which is characterized by comprising the following steps: (1) selecting healthy river crabs; (2) carrying out a third step on the base 10 of the crab^‑ ¹⁰mol/crab dose injection of agonist [ R (-) -TNPA [ ]]The injection of crabs with the weight of 25 to 30 grams is 20 microliters; (3) and observing the fighting state of the river crabs. By injecting an agonist [ R (-) -TNPA ] into river crab]The method can promote the DA protein function in the river crab body, further inhibit the fighting behavior of the river crab, increase the yield of the river crab, and reduce the feed waste and the environmental pollution caused by residual baits.

Description

Method for inhibiting river crab fighting by DA receptor

Technical Field

The invention relates to the technical field of aquaculture, in particular to a method for inhibiting fighting of river crabs by using DA receptors.

Background

River crabs are also commonly known as hairy, crab, freshwater, hairy and prevailing-age crabs, arthropod crustaceans. The meat quality is good, the individual is large, the taste is delicious, and the meat is deeply loved by people.

The head and chest armor formed by combining the head and the chest of the river crab is square and round and hard in texture. The front end of the body is provided with a pair of eyes, and the side surface is provided with two pairs of very sharp crab teeth. The pair of appendages at the forefront end of the crab are chela and full of villi on the surface; there are 4 pairs of feet behind the chela, which are flat and long on the side; the abdominal limb has degenerated. The male and female of the river crab can be distinguished from the abdomen: the female abdomen is round and the male abdomen is triangular. The river crabs are delicious in taste, but male river crabs fight well, the fighting can cause the defects of limbs to influence the quality, can reduce the immunity of the river crabs to influence the survival, can change the feed input or the feed waste due to the influence on the ingestion and reproduction behaviors, possibly can pollute the environment, and reduce the yield of the river crabs.

Disclosure of Invention

The invention aims to provide a method for inhibiting the fighting of river crabs by using DA receptors so as to reduce the fighting of the river crabs and increase the yield of the river crabs.

The invention is realized by the following technical scheme:

a method for inhibiting river crab fighting by using DA receptor comprises the following steps:

(1) selecting healthy river crabs with healthy limbs;

(2) pairing the river crabs in the step (1), respectively singly culturing the paired river crabs in a circulating culture system, feeding the paired river crabs for 1 time every day within a specified time, and performing fighting observation after the paired river crabs are fed for a set number of days;

(3) carrying out a third step on the base 10 of the river crab in the step (2)^-10mol/crab dose injection of agonist;

(4) and (3) selecting 2 male river crabs which are injected in the step (3) and matched with each other, putting the male river crabs into a culture device with water of 10cm, arranging a light-tight partition plate in the middle of the device, putting the two crabs on two sides of the light-tight partition plate respectively, removing the light-tight partition plate after adapting for 10 minutes, observing and recording fighting behaviors between the two crabs by using a camera, and recording the behaviors for 1 hour.

Further, the step (3) of injecting the river crab is performed before feeding.

Further, the weight difference of the river crabs paired in the step (2) is 1-4%.

Further, the injection volume of the crabs with the weight of 25-30 g in the step (3) is 20 microliter.

Furthermore, the agonist is prepared by dissolving the crab normal saline.

The invention principle is as follows:

the applicant discovers through long-term research experiments that the DA level in the haemolymph is obviously changed in the fighting process of the river crabs, the DA can inhibit the fighting of the river crabs, the fighting behavior can be influenced by injecting the DA from an external source, and the DA2 receptor is found to participate in the fighting process. Subsequently, the agonist of DA2 [ R (-) -TNPA ] is found to be capable of obviously inhibiting fighting of river crabs.

Has the advantages that: by injecting an agonist [ R (-) -TNPA ] into the river crabs, the DA protein function in the river crabs can be promoted, so that the fighting behaviors of the river crabs are inhibited, the yield of the river crabs is increased, and the waste of feed and the environmental pollution caused by residual baits are reduced.

Drawings

FIG. 1 is a graph comparing the DA content in the blood plasma before and after fighting.

Fig. 2 is a graph comparing the DA1A content in the body of a river crab before and after fighting.

Fig. 3 is a graph comparing the DA2 content in the body of the river crab before and after fighting.

FIG. 4 is a graph comparing the effect of different concentrations of DA on the fighting time of river crabs.

FIG. 5 is a graph comparing the effect of agonist [ R (-) -TNPA ] on fighting time of river crabs.

Detailed Description

The embodiments of the present invention will be described in detail below with reference to the accompanying drawings: the present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.

Example (b):

(1) selecting healthy river crabs with healthy limbs;

(2) selecting the river crabs in the step (1), pairwise matching (the weight of the river crabs is 20.90 +/-3.87 g, the weight difference is 1-4%) and respectively singly culturing in a culture system (29.0:18.0:19.5cm, length: width: height) (avoiding social behaviors), feeding for 1 time (15: 30-16: 00) within a specified time every day, and performing fighting observation after feeding for a set number of days (7);

(3) carrying out the third step on the base parts 10 of the two river crabs in the step (2)^-10mol/crab dose injection of agonist [ R (-) -TNPA [ ]]Injecting crabs with the weight of 25-30 g into the injection volume of 20 microliter;

(4) selecting two river crabs injected in the step (3), putting the river crabs into a device (20.0:15.5:19.5cm, length: width: height) with water in the device for 10cm, arranging a light-tight partition plate in the middle of the device, respectively placing the two river crabs on two sides of the light-tight partition plate, removing the light-tight partition plate after adapting for 10 minutes, observing and recording fighting behaviors between the two pairs of crabs by using a camera (H.264DVR), and recording the behaviors for 1 hour.

The application is verified by experimental data below:

first, river crabs injected with no drug were observed, and the DA content in the plasma before and after fighting was as shown in fig. 1: the DA content in the blood plasma of the river crab is reduced after fighting.

Secondly, injecting DA into the bodies of the river crabs through an external source to test the fighting behaviors of the river crabs, as shown in figures 2 and 3: it can be seen that the DA1A and DA2 receptors are expressed in blood cells, eye handles, brain thoracic ganglia and intestines all varied.

Thirdly, recording the effect of different concentrations of DA on the fighting time, as shown in table 1 below and fig. 4: injection 10^-6The mol DA remarkably reduces the approaching times and fighting times between two river crabs, and compared with a control group and a normal saline group, the fighting times and time after DA injection are both obviously reduced.

TABLE 1

Fourthly, injection 10^-10mol/crab dose agonist [ R (-) -TNPA]The fighting behavior of the river crab can be obviously inhibited, as shown in the following table 2 and fig. 5:

TABLE 2

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A method for inhibiting the fighting of river crabs by using DA receptors is characterized by comprising the following steps:

(1) selecting healthy river crabs with healthy limbs;

(2) pairing the river crabs in the step (1), respectively singly culturing the paired river crabs in a culture system, feeding the paired river crabs for 1 time in a specified time every day, and performing fighting observation after the paired river crabs are fed for a set number of days;

(3) carrying out the third step of the foot base parts 10 on the river crabs paired in pairs in the step (2)^-10mol/crab dose injection of agonist [ R (-) -TNPA [ ]]Injecting crabs with the weight of 25-30 g into the injection volume of 20 microliter;

(4) selecting 2 river crabs which are injected and matched in the step (3), putting the river crabs into a device with water of 10cm, arranging a light-tight clapboard in the middle of the device, respectively putting two river crabs on two sides of the light-tight clapboard, removing the light-tight clapboard after adapting for 10 minutes, observing and recording fighting behaviors between the two pairs of male river crabs by using a camera, and recording the behaviors for 1 hour,

the step (2) is carried out before feeding.

2. The method for inhibiting fighting of river crabs with DA receptors according to claim 1, wherein the body weight of the paired river crabs in the step (2) is different by 1 to 4%.

3. The method of claim 1, wherein the agonist is formulated by dissolving in normal saline.