CN116803254A - Method for distinguishing beautyberry and non-beautyberry Eriocheir sinensis populations - Google Patents
Method for distinguishing beautyberry and non-beautyberry Eriocheir sinensis populations Download PDFInfo
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- CN116803254A CN116803254A CN202310237375.9A CN202310237375A CN116803254A CN 116803254 A CN116803254 A CN 116803254A CN 202310237375 A CN202310237375 A CN 202310237375A CN 116803254 A CN116803254 A CN 116803254A
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 241000371997 Eriocheir sinensis Species 0.000 title claims abstract description 23
- 235000017595 Callicarpa japonica Nutrition 0.000 title description 4
- 240000003690 Callicarpa japonica Species 0.000 title description 4
- 241000238557 Decapoda Species 0.000 claims abstract description 113
- 238000012360 testing method Methods 0.000 claims abstract description 24
- 238000002474 experimental method Methods 0.000 claims abstract description 19
- 230000004888 barrier function Effects 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 230000006399 behavior Effects 0.000 claims description 17
- 230000003993 interaction Effects 0.000 claims description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 4
- 230000006978 adaptation Effects 0.000 claims description 3
- 230000009194 climbing Effects 0.000 claims description 3
- 238000011156 evaluation Methods 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 230000002452 interceptive effect Effects 0.000 claims 2
- 238000005192 partition Methods 0.000 claims 2
- 241000371986 Eriocheir Species 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 3
- 206010071602 Genetic polymorphism Diseases 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000003445 biliary tract Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The application discloses a method for distinguishing a fighting and non-fighting Eriocheir sinensis group, which comprises the following steps: (1) selecting crabs with intact limbs in growth; (2) biliary testing: evaluating the biliary quantity of the crabs through a barrier crossing experiment; (3) aggressiveness test: evaluating aggressiveness of the crabs through a mirror image experiment; (4) distinguishing the good crabs from the non-good crabs: and distinguishing the best crabs from the non-best crabs according to the biliary test result and the aggressive test result. The application establishes two populations of the eriocheir sinensis and the non-eriocheir sinensis through a scientific method, and provides materials for genetic polymorphism analysis between the two populations and further for molecular breeding of the eriocheir sinensis so as to breed the non-eriocheir sinensis. The method provided by the application can distinguish the crabs from the non-crabs, and can adopt a low-density cultivation mode for the crabs so as to realize the purpose of rapid growth; the method can be used for raising the non-live crabs in a high-density manner, and the raising manner of separate raising greatly improves the yield and the output value.
Description
Technical Field
The application relates to the technical field of production and cultivation of eriocheir sinensis, in particular to a method for distinguishing a group of eriocheir sinensis from a group of non-eriocheir sinensis.
Background
In recent years, the cultivation of the eriocheir sinensis is transformed from lake purse net cultivation to pond ecological cultivation, and a new green transformation mode is started. Under the high-density pond culture condition, the fight behavior is aggravated. The lack of appendages or physical injury caused by fight can cause the reduction of immunocompetence, the reduction of foraging efficiency and mating success rate, and the like to eat, and seriously affect the survival, growth, quality and economic value of the eriocheir sinensis. In addition, because limbs are defective and cannot be ingested normally, a large amount of baits can be deposited on the bottom of the pond to pollute the cultivation environment, and the green ecological cultivation level is reduced.
The fighting behavior also greatly limits the yield and the output value of the eriocheir sinensis pond ecological breeding industry. Under the condition of pond culture, the fight behavior leads to the increase of the death rate of the eriocheir sinensis, the decrease of welfare level and the decrease of yield and quality. Therefore, how to scientifically and effectively control the fight phenomenon becomes one of the problems to be solved in the production of the eriocheir sinensis.
Disclosure of Invention
The application provides a method for distinguishing a fighting and non-fighting Eriocheir sinensis population, which aims to establish the fighting and non-fighting Eriocheir sinensis population, and can be used for analyzing gene polymorphism between the fighting and non-fighting crabs and identifying single nucleotide polymorphism SNP sites related to the fighting behavior of the crabs and providing materials for molecular breeding of the crabs.
The application is realized by the following technical scheme:
a method for distinguishing a group of vanity and non-vanity eriocheir sinensis, comprising the steps of:
(1) Selecting growing crabs with intact limbs;
(2) Biliary testing: evaluating the biliary quantity of the crabs through a barrier crossing experiment;
(3) Aggressiveness test: evaluating aggressiveness of the crabs through a mirror image experiment;
(4) Distinguishing the good crabs from the non-good crabs: and distinguishing the best crabs from the non-best crabs according to the biliary test result and the aggressive test result.
The device for the barrier net crossing experiment in the biliary test in the step (2) comprises a square water tank I and a barrier net, wherein the water surface in the square water tank I has a certain height;
as the preferable scheme, the block comprises iron wires and fishing nets, the block can simulate the crab culture environment more truly, the block is convenient for the crabs to climb and span, the square water tank I belongs to the strange environment for the crabs, and meanwhile, the body is exposed to the air in the crab-climbing process, so that the risk is a risk for the crabs, and the amount of the gall of the crabs is quantified by counting the number of times the crabs climb over the block before adapting to the environment.
Preferably, the number of times of crossing the barrier net in a certain time is counted, and the greater the number of times of crossing, the greater the biliary quantity.
As a preferable scheme, the mirror image experiment device in the aggressiveness test in the step (3) comprises a square water tank II and a plane mirror clung to one side of the square water tank II, the times of interaction behaviors of the crabs and the plane mirror are counted, and the aggressiveness of the crabs is quantized.
Preferably, the interaction behavior comprises approaching, touching and lifting the chelating limb, and the sum of the three interaction behaviors is recorded as the number of aggressiveness, and the more the number is, the stronger the aggressiveness is.
As a preferred scheme, the step (4) is used for distinguishing the good crabs from the non-good crabs, wherein the biliary quantity (i.e. the number of times of barrier crossing) of the good crabs is significantly higher than that of the non-good crabs, and the aggressiveness of the good crabs is significantly higher than that of the non-good crabs. The fighting character of the crabs is determined by characteristics of two characters, namely the biliary quantity and the aggressiveness, and the fighting crabs are the crabs with large biliary quantity and aggressiveness.
Preferably, the accuracy of the fighting crabs and the non-fighting crabs obtained in the step (4) is verified through evaluation of fighting experiments (namely the contact times and the single fighting time).
As the preferred scheme, the device of experiment of fighting includes square water tank three of taking the baffle to and vertically place in square water tank three top's camera, places two crabs in square water tank three's both sides respectively, takes off the baffle after adaptation ten minutes, records the condition of fighting of crab in one hour through the camera.
As a preferable scheme, the fight behaviors between the good crabs and between the non-good crabs and the non-good crabs are observed, the single fight time between the good crabs is found to be obviously higher than the fight time between the non-good crabs, and meanwhile, the contact times between the good crabs are obviously higher than the contact times between the non-good crabs, so that the fight behaviors of the screened good crabs can be verified to be stronger than those of the non-good crabs.
The beneficial effects are that:
(1) The application discloses a method for distinguishing a fine-leaved crab group from a non-fine-leaved crab group, which is used for further distinguishing and analyzing gene polymorphism between fine-leaved crabs and non-fine-leaved crabs through a scientific method, identifying Single Nucleotide Polymorphism (SNP) sites associated with the scuttling behaviors of the fine-leaved crabs so as to provide important group materials for molecular breeding of the fine-leaved crabs, and finally culturing new fine-leaved crabs (non-fine-leaved crabs) as main breeding objects.
(2) The method provided by the application can be used for distinguishing the crabs from the non-crabs, and in the field of crab breeding, a low-density breeding mode can be adopted for the crabs so as to realize the purpose of rapid growth; the method can be used for raising the non-fighting crabs in a high-density manner, saves resources on the premise of not influencing the growth speed of the crabs, and greatly improves the yield and the output value.
Drawings
Fig. 1 is a schematic structural view of a barrier net-crossing experimental apparatus according to an embodiment of the present application.
FIG. 2 is a schematic diagram of a mirror image experimental apparatus in an embodiment of the application.
Fig. 3 is a graph showing the distinction of a good crab and a non-good crab according to the biliary test result and the aggressive test result of the present application.
Fig. 4 is a schematic structural diagram of a fight experimental device in an embodiment of the application.
Detailed Description
Embodiments of the present application will be described in detail below with reference to the attached drawings: the present embodiment is implemented on the premise of the technical scheme of the present application, and a detailed implementation manner and a specific operation process are provided, but the protection scope of the present application is not limited to the following embodiments.
A method for distinguishing a group of vanity and non-vanity eriocheir sinensis, comprising the steps of:
(1) Selecting crab larvae with weight of 30-40 g and complete limbs;
(2) Biliary testing: evaluating the biliary quantity of the crabs through a barrier crossing experiment;
the device for the barrier net crossing experiment in the biliary tract test in the step (2) comprises a square water tank I (23 multiplied by 18 multiplied by 30 cm) and a barrier net, wherein the height of the water surface in the square water tank I is 10cm.
The block comprises iron wires and fishing nets (17 multiplied by 10 cm), the setting of the block can simulate the crab culture environment more truly, the crab climbing and crossing are facilitated, the square water tank I belongs to a strange environment for crabs, meanwhile, the body is exposed to the air in the crab crossing process, and the risk is high for crabs, so that the number of times of crossing the block before adapting to the environment (after adapting to the square water tank I, the number of times of crossing is obviously reduced) is counted, and the block is used for quantifying the biliary quantity of the crabs. Counting the number of times of crossing the barrier net within 30 minutes, wherein the greater the number of times of crossing, the greater the biliary quantity, the more aggressive character of the crab is determined by the characteristics of biliary quantity and aggressive character, and the more aggressive crab is. As shown in fig. 1.
(3) Aggressiveness test: evaluating aggressiveness of the crabs through a mirror image experiment;
the mirror image experiment device in the aggressiveness test in the step (3) comprises a square water tank II (23 multiplied by 18 multiplied by 30 cm) and a plane mirror (20 multiplied by 10 cm) which is tightly attached to one side of the square water tank II, the times of interaction actions of the crabs and the plane mirror are counted, the aggressiveness of the crabs is quantified, the interaction actions comprise approaching, contacting and lifting chelating limbs, the sum of the three interaction actions is recorded as the number of aggressiveness, and the aggressiveness is stronger as the number is larger. As shown in fig. 2.
(4) Distinguishing the good crabs from the non-good crabs: and distinguishing the best crabs from the non-best crabs according to the biliary test result and the aggressive test result.
The step (4) is to distinguish the good crabs from the non-good crabs, wherein the biliary quantity (the number of times of barrier crossing) of the good crabs is obviously higher than that of the non-good crabs, and meanwhile, the aggressiveness of the good crabs is obviously higher than that of the non-good crabs. As shown in fig. 3, a represents a biliary quantity and B represents an aggressiveness.
Evaluation of the overdriving experiments (number of contacts and single fighting time) was performed to verify the accuracy of the resultant and non-fighting crabs distinguished in the above examples.
The device for fighting experiments comprises a square water tank III (23X 18X 30 cm) with a baffle plate (18X 25 cm) and a camera vertically arranged above the square water tank III, wherein two crabs are respectively arranged on two sides of the square water tank III, the baffle plate is taken down after ten minutes of adaptation, and the fighting condition of the crabs in one hour is recorded through the camera. As shown in fig. 4.
The fight behaviors between the good crabs and the non-good crabs and between the non-good crabs are observed, the single fight time between the good crabs is found to be obviously higher than the fight time between the non-good crabs (p < 0.01), and meanwhile, the contact times between the good crabs are obviously higher than the contact times between the non-good crabs (p < 0.05), so that the fight behaviors of the selected good crabs can be verified to be stronger than those of the non-good crabs.
The foregoing has shown and described the basic principles and main features of the present application and the advantages of the present application. It will be understood by those skilled in the art that the present application is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present application, and various changes and modifications may be made without departing from the spirit and scope of the application, which is defined in the appended claims. The scope of the application is defined by the appended claims and equivalents thereof.
Claims (10)
1. A method for distinguishing a group of fighting and non-fighting eriocheir sinensis, comprising the steps of:
(1) Selecting growing crabs with intact limbs;
(2) Biliary testing: evaluating the biliary quantity of the crabs through a barrier crossing experiment;
(3) Aggressiveness test: evaluating aggressiveness of the crabs through a mirror image experiment;
(4) Distinguishing the good crabs from the non-good crabs: and distinguishing the best crabs from the non-best crabs according to the biliary test result and the aggressive test result.
2. The method of claim 1, wherein the device for the barrier crossing experiment in the biliary test in step (2) comprises a square water tank I and a barrier, wherein the water surface in the square water tank I has a certain height.
3. The method for distinguishing the fine and non-fine chinese mitten crabs according to claim 1, characterized in that the barrier net comprises iron wires and fishing nets, the barrier net is arranged to simulate the crab culture environment more truly, the climbing and crossing of crabs are facilitated, the square water tank is a strange environment for crabs, the body is exposed to the air in the process of the crab climbing, and the risk is a risk for crabs, so that the amount of the gall of the crabs is quantified by counting the number of times the crabs climb the barrier net before adapting to the environment.
4. The method of claim 1, wherein the number of times the net is turned over in a certain period of time is counted, and the greater the number of times the net is turned over, the greater the amount of the gall is.
5. The method for distinguishing the fighting and non-fighting Eriocheir sinensis groups according to claim 1, wherein the mirror image experimental device in the aggressive test in the step (3) comprises a square water tank II and a plane mirror attached to one side of the square water tank II, and the number of times of interaction behaviors of the crabs and the plane mirror is counted to quantify the aggressiveness of the crabs.
6. The interactive behavior comprises approaching, touching and lifting the chelating limb, and the sum of the three interactive behaviors is recorded as the number of aggressiveness, and the more the number is, the stronger the aggressiveness is.
7. The method of claim 1, wherein the step (4) distinguishes between a good crab and a non-good crab, wherein the good crab has a significantly higher biliary content than the non-good crab, and wherein the good crab has a significantly higher aggressiveness than the non-good crab.
8. The method of claim 1, wherein the accuracy of the fighting and non-fighting crabs obtained in step (4) is verified by evaluation of a fighting experiment.
9. The method for distinguishing a group of fighting and non-fighting eriocheir sinensis as claimed in claim 1, wherein the device for fighting experiment comprises a square water tank three with a partition plate and a camera vertically arranged above the square water tank three, wherein two crabs are respectively arranged on two sides of the square water tank three, the partition plate is taken down after ten minutes of adaptation, and the fighting condition of the crabs within one hour is recorded through the camera.
10. The method for distinguishing between a population of aggressive and non-aggressive eriocheirs according to claim 1, wherein the behavior of fighting between aggressive and non-aggressive crabs is observed, and a single time of fighting between aggressive crabs is found to be significantly higher than the time of fighting between non-aggressive crabs, and the number of contacts between aggressive crabs is significantly higher than the number of contacts between non-aggressive crabs, so that the screened aggressive crabs can be verified to have stronger fighting behavior than non-aggressive crabs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310237375.9A CN116803254A (en) | 2023-03-14 | 2023-03-14 | Method for distinguishing beautyberry and non-beautyberry Eriocheir sinensis populations |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310237375.9A CN116803254A (en) | 2023-03-14 | 2023-03-14 | Method for distinguishing beautyberry and non-beautyberry Eriocheir sinensis populations |
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| CN116803254A true CN116803254A (en) | 2023-09-26 |
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| CN202310237375.9A Pending CN116803254A (en) | 2023-03-14 | 2023-03-14 | Method for distinguishing beautyberry and non-beautyberry Eriocheir sinensis populations |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110934099A (en) * | 2019-11-08 | 2020-03-31 | 上海海洋大学 | Method for inhibiting river crab fighting by DA receptor |
| CN111950598A (en) * | 2020-07-19 | 2020-11-17 | 中国海洋大学 | Method for individually classifying swimming crab groups based on K-Means algorithm and application |
| CN112753623A (en) * | 2021-01-22 | 2021-05-07 | 交通运输部天津水运工程科学研究所 | Experimental device and testing method for brave and exploration behaviors of fishes |
| CN115669584A (en) * | 2022-09-28 | 2023-02-03 | 中国海洋大学 | Parent breeding method for portunus trituberculatus based on aggressive character |
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2023
- 2023-03-14 CN CN202310237375.9A patent/CN116803254A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110934099A (en) * | 2019-11-08 | 2020-03-31 | 上海海洋大学 | Method for inhibiting river crab fighting by DA receptor |
| CN111950598A (en) * | 2020-07-19 | 2020-11-17 | 中国海洋大学 | Method for individually classifying swimming crab groups based on K-Means algorithm and application |
| CN112753623A (en) * | 2021-01-22 | 2021-05-07 | 交通运输部天津水运工程科学研究所 | Experimental device and testing method for brave and exploration behaviors of fishes |
| CN115669584A (en) * | 2022-09-28 | 2023-02-03 | 中国海洋大学 | Parent breeding method for portunus trituberculatus based on aggressive character |
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