CN111727915B - Method for tracing heavy metal enrichment way of hanging-cultured bivalve shellfish - Google Patents
Method for tracing heavy metal enrichment way of hanging-cultured bivalve shellfish Download PDFInfo
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- 235000015170 shellfish Nutrition 0.000 title claims abstract description 46
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 26
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 116
- 229910052709 silver Inorganic materials 0.000 claims abstract description 116
- 239000004332 silver Substances 0.000 claims abstract description 116
- 239000013535 sea water Substances 0.000 claims abstract description 70
- BQCADISMDOOEFD-OUBTZVSYSA-N silver-109 Chemical compound [109Ag] BQCADISMDOOEFD-OUBTZVSYSA-N 0.000 claims abstract description 21
- BQCADISMDOOEFD-BJUDXGSMSA-N silver-107 Chemical compound [107Ag] BQCADISMDOOEFD-BJUDXGSMSA-N 0.000 claims abstract description 18
- 239000000700 radioactive tracer Substances 0.000 claims abstract description 8
- 239000002609 medium Substances 0.000 claims description 36
- 210000001519 tissue Anatomy 0.000 claims description 33
- 239000002245 particle Substances 0.000 claims description 30
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 11
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 9
- 210000003205 muscle Anatomy 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 239000003463 adsorbent Substances 0.000 claims description 7
- 210000004974 shell Anatomy 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000009313 farming Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000013618 particulate matter Substances 0.000 claims description 4
- 239000003957 anion exchange resin Substances 0.000 claims description 3
- 229920001429 chelating resin Polymers 0.000 claims description 3
- 210000002816 gill Anatomy 0.000 claims description 3
- 230000037361 pathway Effects 0.000 claims 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000007547 defect Effects 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 3
- 241000237502 Ostreidae Species 0.000 description 10
- 235000020636 oyster Nutrition 0.000 description 10
- 210000003467 cheek Anatomy 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 238000001948 isotopic labelling Methods 0.000 description 1
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- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a method for tracing a heavy metal enrichment way of hanging-cultured bivalve shellfish, which comprises the steps of adopting a stable silver isotope to mark a selected medium to obtain a marked medium, wherein the medium is contained in a bivalve shellfish culture environment; the stable silver isotopes include silver 107 and silver 109. According to the scheme provided by the application, the silver stable isotopes (107Ag and 109Ag) are used for tracing the adsorption and enrichment conditions of the silver in the suspended double-shell shellfish to different environment media (seawater, suspended particulate matters and baits), so that the source of the silver entering the suspended double-shell shellfish body is obtained, and the distribution conditions of different tissues of the silver from different sources in the suspended double-shell shellfish body are obtained simultaneously. The scheme that this application provided has overcome the defect that utilizes the unable traceability of single isotope, adopts the simple and easily obtained silver stable isotope of isotope constitution as the tracer, provides technical support for ocean heavy metal silver pollution prevention and control.
Description
Technical Field
The invention relates to the technical field of heavy metal enrichment and tracing, in particular to a method for tracing a heavy metal enrichment way of hanging-cultured bivalve shellfish.
Background
Heavy metal pollution in the ocean has been long valued by people due to its strong toxicity, biological amplification and difficult degradability. Silver is a heavy metal polluting element that has been of increasing interest in recent years, silver being a non-essential element for living things and being second only to mercury in toxicity to marine life, especially invertebrates. The silver enrichment factor of the suspended bivalve shellfish such as oyster is higher than that of zinc and copper, and the suspension bivalve shellfish is an indicator organism for researching silver pollution.
How to trace the source and the way of enriching the heavy metal of the suspended-culture bivalve shellfish is the key of preventing and controlling the heavy metal pollution of the mariculture, and the isotope and the ratio thereof as the 'fingerprint' of the heavy metal can not be replaced in the migration and the transformation of the trace heavy metal. Compared with radioactive isotopes, stable isotopes have the advantages of no radiation, no pollution, no decay, easy availability of isotopes, simple determination and the like, and are gradually and widely applied. Some studies have been conducted to trace heavy metal contamination of bivalve shellfish (e.g., oyster) seafood using stable isotopes (e.g., cadmium, zinc, copper, etc.). However, the research on the source of heavy metal pollution of marine products traced by stable isotopes of silver is not available at present.
Disclosure of Invention
The invention provides a method for tracing a heavy metal enrichment way of hanging-cultured bivalve shellfish.
The invention provides the following scheme:
a method for tracing a heavy metal enrichment way of hanging-cultured bivalve shellfish comprises the following steps:
adopting a stable silver isotope to mark a selected medium to obtain a marked medium, wherein the medium is contained in a bivalve shellfish culture environment; the stable silver isotopes include silver 107 and silver 109;
exposing the bivalves to a farming environment comprising the marking medium; and determining the types of stable silver isotopes of different tissues of the bivalve after a certain time, and determining a heavy metal enrichment way of the bivalve according to the types of the stable silver isotopes.
Preferably: the medium comprises seawater, suspended particulate matters and bait.
Preferably: the medium is seawater, the marking medium is first marked seawater, and the preparation method of the first marked seawater comprises the following steps:
collecting a seawater sample, filtering, and adding a proper amount of silver selective adsorbent to adsorb silver in the seawater to obtain silver-free seawater;
and mixing a proper amount of the stable silver isotope of the selected species with the silver-free seawater, and oscillating for 22-25 hours to obtain the first marked seawater.
Preferably: the silver selective adsorbent comprises a chelating resin and/or an anion exchange resin and/or a chelating agent.
Preferably: the medium is suspended particles, the marking medium is marked suspended particles, and the preparation method of the marked suspended particles comprises the following steps:
collecting suspended particles in seawater, cleaning and drying the suspended particles, and mixing the cleaned and dried suspended particles with second marked seawater, wherein the second marked seawater and the first marked seawater are marked by different kinds of stable silver isotopes;
and oscillating for 6-8 days to enable the stable silver isotope contained in the second marked seawater to be adsorbed on the suspended particles to obtain marked suspended particles.
Preferably: the medium is bait, the marking medium is marking bait, and the preparation method of the marking bait comprises the following steps:
and placing the selected bait into a selected stable silver isotope labeled culture medium different from the stable silver isotope contained in the first labeled seawater for culture to obtain the labeled bait.
Preferably: the bait is selected chlorella, the chlorella is placed in a selected stable silver isotope labeled culture medium which is different from the stable silver isotope type contained in the first labeled seawater for culture, and the chlorella is centrifuged to obtain the labeled bait after growing to a certain concentration.
Preferably: determining the types of stable silver isotopes of different tissues of the bivalve after a certain time, and determining a path for enriching heavy metals in the bivalve according to the types of the stable silver isotopes, wherein the path comprises the following steps:
determining the types of stable silver isotopes of different tissues of the bivalve after a certain time and the content of the stable silver isotopes in each tissue, determining the heavy metal enrichment way of the bivalve according to the types of the stable silver isotopes, and determining the distribution condition of silver from different ways in each tissue of the bivalve according to the content of the stable silver isotopes in each tissue.
Preferably: the bivalve shellfish different tissues include the digestive tract, gills, muscles and shells.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects:
the method for tracing the heavy metal enrichment way of the suspended double-shell shellfish can be realized, and in an implementation mode, the method can comprise the steps of adopting a stable silver isotope to mark a selected medium to obtain a marked medium, wherein the medium is contained in the culture environment of the double-shell shellfish; the stable silver isotopes include silver 107 and silver 109; exposing the bivalves to a farming environment comprising the marking medium; and determining the types of stable silver isotopes of different tissues of the bivalve after a certain time, and determining a heavy metal enrichment way of the bivalve according to the types of the stable silver isotopes. According to the scheme provided by the application, the silver stable isotopes (107Ag and 109Ag) are used for tracing the adsorption and enrichment conditions of the silver in the suspended double-shell shellfish to different environment media (seawater, suspended particulate matters and baits), so that the source of the silver entering the suspended double-shell shellfish body is obtained, and the distribution conditions of different tissues of the silver from different sources in the suspended double-shell shellfish body are obtained simultaneously. The scheme that this application provided has overcome the defect that utilizes the unable traceability of single isotope, adopts the simple and easily obtained silver stable isotope of isotope constitution as the tracer, provides technical support for ocean heavy metal silver pollution prevention and control.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a graph showing the enrichment ratio of silver 107 in the tissues of oysters in the first experimental group according to the embodiment of the present invention;
FIG. 2 is a graph showing the enrichment ratio of silver 109 in each tissue of oysters in the first experimental group according to the embodiment of the present invention;
fig. 3 is a schematic diagram of the enrichment ratio of silver 109 in each tissue of oysters in experiment group two according to the embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments that can be derived by one of ordinary skill in the art from the embodiments given herein are intended to be within the scope of the present invention.
Examples
The embodiment of the invention provides a method for tracing a heavy metal enrichment way of hanging-cultured bivalve shellfish, which comprises the steps of adopting a stable silver isotope to mark a selected medium to obtain a marked medium, wherein the medium is contained in a bivalve shellfish culture environment; the stable silver isotopes include silver 107 and silver 109; exposing the bivalves to a farming environment comprising the marking medium; and determining the types of stable silver isotopes of different tissues of the bivalve after a certain time, and determining a heavy metal enrichment way of the bivalve according to the types of the stable silver isotopes.
Silver shares 28 isotopes (from silver 93 to silver 130) with most isotopes having half-lives of less than three minutes. According to the method, two isotopes of silver 107 and silver 109 are selected and used as the tracer for marking silver, compared with traditional heavy metals such as cadmium, zinc and the like, the silver is an unnecessary element for biological growth, and the stable isotope of the silver is only silver 107(107Ag) and silver 109(109Ag), and the single isotope is easy to prepare and obtain and is an ideal model element for tracing the enriched heavy metals of shellfish organisms.
In particular, because the culture environment of the bivalve shellfish comprises a plurality of mediums, the mediums can comprise a plurality of mediums, and the scheme provided by the application can select several typical mediums, particularly, the mediums comprise seawater, suspended particulate matters and bait.
Further, the medium is seawater, the marking medium is first marking seawater, and the preparation method of the marking seawater comprises the following steps:
collecting a seawater sample, filtering, and adding a proper amount of silver selective adsorbent to adsorb silver in the seawater to obtain silver-free seawater; the silver selective adsorbent comprises a chelating resin and/or an anion exchange resin and/or a chelating agent.
Mixing a proper amount of stable silver isotopes of a selected variety with the silver-free seawater, and shaking for 22-25 hours to obtain first marked seawater.
The medium is suspended particles, the marking medium is marked suspended particles, and the preparation method of the marked suspended particles comprises the following steps:
collecting suspended particles in seawater, cleaning and drying the suspended particles, and mixing the cleaned and dried suspended particles with second marked seawater, wherein the second marked seawater and the first marked seawater are marked by different kinds of stable silver isotopes; it is conceivable that the second marked seawater may be prepared by the same preparation method as the first marked seawater, and different kinds of stable silver isotopes may be added during the preparation process.
And oscillating for 6-8 days to enable the stable silver isotope contained in the second marked seawater to be adsorbed on the suspended particles to obtain marked suspended particles.
The medium is bait, the marking medium is marking bait, and the preparation method of the marking bait comprises the following steps:
and placing the selected bait into a selected stable silver isotope labeled culture medium different from the stable silver isotope contained in the first labeled seawater for culture to obtain the labeled bait.
Specifically, the bait is selected chlorella, the chlorella is placed in a selected stable silver isotope labeled culture medium which is different from the stable silver isotope type contained in the first labeled seawater for culture, and the chlorella is centrifuged to obtain the labeled bait after growing to a certain concentration.
In order to determine the distribution condition of silver in each tissue of the bivalve, the types of stable silver isotopes of different tissues of the bivalve and the content of the stable silver isotopes in each tissue are determined after a certain time, the path of enriching heavy metal of the bivalve is determined according to the types of the stable silver isotopes, and the distribution condition of silver from different paths in each tissue of the bivalve is determined according to the content of the stable silver isotopes in each tissue. The bivalve shellfish different tissues include the digestive tract, gills, muscles and shells.
The method provided by the application is described in detail by specific implementation methods.
a. Preparing the seawater marked by the silver isotope. Collecting seawater samples, filtering, and adding a proper amount of silver selective adsorbent to adsorb silver in the seawater to obtain silver-free seawater. Mixing a proper amount of 107Ag with the silver-free seawater, and shaking for 24 hours to obtain 107Ag marked seawater (first marked seawater).
b. Preparing silver isotope labeled suspended particles. Collecting suspended particles in seawater, cleaning, drying, mixing with 109Ag marked seawater (second marked seawater), and shaking for 7 days to make 109Ag be fully adsorbed on the particles to obtain 109Ag marked suspended particles.
c. Preparing the silver isotope labeling bait. Selecting chlorella strain as bait for bivalve shellfish, and culturing in culture medium marked by 109 Ag. When the chlorella grows to a certain concentration, centrifuging to obtain 109Ag mark bait;
d. silver exposure experiments.
Selecting 3 colorless containers, simulating a natural culture environment for hanging and culturing bivalve shellfish, placing healthy oysters in each container, and carrying out an exposure experiment by using Ag-free seawater as a culture substrate.
The 1 st container is a control group: silver-free seawater, natural suspended particles and natural chlorella.
The 2 nd vessel is experimental group one: marking seawater by 107Ag, marking suspended particles by 109Ag, and natural chlorella.
The 3 rd vessel is experimental group two: 107Ag marks seawater, suspended particles and 109Ag marks chlorella.
The culture medium amount decreases with the number of times of collecting samples, taking bivalve shellfish from each container after exposure time of 0, 7, 14, 21, 28 days, and dissecting to obtain tissue samples of cheek, digestive tract, muscle, and shell;
e. and (4) measuring silver. The contents of 107Ag and 109Ag in different tissues were determined by Inductively Coupled Plasma Mass Spectrometer (ICPMS).
f. Determining a heavy metal enrichment way of the hanging-cultured bivalve shellfish.
And (4) according to the content data of 107Ag and 109Ag in different tissues obtained in the process e, comparing corresponding data in an exposed environment, and determining the heavy metal enrichment way of the bivalve shellfish as shown in the table 1. And the enrichment ratio of silver in each organ (ng. g) was calculated as shown in fig. 1 to 3-1) Time (d).
TABLE 1 tissue types comprising silver isotopes
| Group of | Whether silver was detected in the tissue 107 | Whether silver is detected 109 in tissue |
| Control group | Whether or not | Whether or not |
| Experiment set 1 | Is that | Is that |
| Experiment group two | Is that | Is that |
As can be seen from Table 1, bivalve shellfish can enter the body through three routes, seawater, suspended particulate matter and bait.
As can be seen from the attached figures 1-3, all organs of the oyster can absorb and enrich silver from seawater, suspended particles and bait, and the enrichment rate is larger along with the time, so that the typical enrichment effect is shown.
Referring to fig. 1, for the silver in seawater, the enrichment rate of the silver in each organ of the oyster is sequentially digestive tract, cheek, muscle and shell, wherein the enrichment rate in the muscle gradually exceeds the cheek and the digestive tract after 2 times of sampling.
Referring to fig. 2, for the silver in the suspended particles, the enrichment rate of the silver in each organ of the oyster is sequentially digestive tract, cheek, muscle and shell.
Referring to fig. 3, for the silver in the bait, the enrichment rates of the silver in all organs of the oyster are sequentially digestive tract, muscle, cheek and shell, wherein the enrichment rate in the muscle gradually exceeds the digestive tract after 2 times of sampling.
In a word, the scheme provided by the application utilizes the silver stable isotopes (107Ag and 109Ag) to trace the adsorption and enrichment conditions of the silver in the suspended-culture bivalve shellfish to different environment media (seawater, suspended particulate matters and baits), so that the source of the silver entering the suspended-culture bivalve shellfish body is obtained, and the distribution conditions of different tissues of the silver from different sources in the suspended-culture bivalve shellfish body are obtained at the same time. The scheme that this application provided has overcome the defect that utilizes the unable traceability of single isotope, adopts the simple and easily obtained silver stable isotope of isotope constitution as the tracer, provides technical support for ocean heavy metal silver pollution prevention and control.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.
Claims (7)
1. A method for tracing a heavy metal enrichment way of hanging double-shell shellfish, which is characterized by comprising the following steps:
adopting a stable silver isotope to mark a selected medium to obtain a marked medium, wherein the medium is contained in a bivalve shellfish culture environment; the stable silver isotopes include silver 107 and silver 109; the medium comprises seawater, suspended particulate matters and bait; the medium is seawater, the marking medium is first marked seawater, and the preparation method of the first marked seawater comprises the following steps:
collecting a seawater sample, filtering, and adding a proper amount of silver selective adsorbent to adsorb silver in the seawater to obtain silver-free seawater;
mixing a proper amount of stable silver isotopes of a selected variety with silver-free seawater, and oscillating for 22-25 hours to obtain first marked seawater;
exposing the bivalves to a farming environment comprising the marking medium; and determining the types of stable silver isotopes of different tissues of the bivalve after a certain time, and determining a heavy metal enrichment way of the bivalve according to the types of the stable silver isotopes.
2. The method for enriching heavy metal paths in tracer suspended double-shell shellfish according to claim 1, wherein the silver selective adsorbent comprises chelating resin and/or anion exchange resin and/or chelating reagent.
3. The method for enriching the heavy metal path of the tracer suspended double-shell shellfish, as recited in claim 1, wherein the medium is suspended particulate matter, the marked medium is marked suspended particulate matter, and the preparation method of the marked suspended particulate matter comprises the following steps:
collecting suspended particles in seawater, cleaning and drying the suspended particles, and mixing the cleaned and dried suspended particles with second marked seawater, wherein the second marked seawater and the first marked seawater are marked by different kinds of stable silver isotopes;
and oscillating for 6-8 days to enable the stable silver isotope contained in the second marked seawater to be adsorbed on the suspended particles to obtain marked suspended particles.
4. The method for enriching the heavy metal path of the tracer suspended double-shell shellfish according to claim 1, wherein the medium is bait, the marking medium is marking bait, and the preparation method of the marking bait comprises the following steps:
and placing the selected bait into a selected stable silver isotope labeled culture medium different from the stable silver isotope contained in the first labeled seawater for culture to obtain the labeled bait.
5. The method for tracing the heavy metal enrichment pathway of the double-shell shellfish cultured in the manner as claimed in claim 4, wherein the bait is selected chlorella, the chlorella is cultured in a selected culture medium labeled with a stable silver isotope of a different kind from the stable silver isotope contained in the first labeled seawater, and the chlorella is centrifuged to obtain the labeled bait after growing to a certain concentration.
6. The method for tracing the heavy metal enrichment pathway of the suspended double-shell shellfish as claimed in claim 1, wherein the determining the kinds of the stable silver isotopes of different tissues of the double-shell shellfish after a certain period of time and the determining the heavy metal enrichment pathway of the double-shell shellfish according to the kinds of the stable silver isotopes comprises:
determining the types of stable silver isotopes of different tissues of the bivalve after a certain time and the content of the stable silver isotopes in each tissue, determining the heavy metal enrichment way of the bivalve according to the types of the stable silver isotopes, and determining the distribution condition of silver from different ways in each tissue of the bivalve according to the content of the stable silver isotopes in each tissue.
7. The method for enriching heavy metal paths in tracer suspended double-shell shellfish according to claim 6, characterized in that different tissues of the double-shell shellfish comprise digestive tract, gill, muscle and shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010611327.8A CN111727915B (en) | 2020-06-30 | 2020-06-30 | Method for tracing heavy metal enrichment way of hanging-cultured bivalve shellfish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010611327.8A CN111727915B (en) | 2020-06-30 | 2020-06-30 | Method for tracing heavy metal enrichment way of hanging-cultured bivalve shellfish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111727915A CN111727915A (en) | 2020-10-02 |
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