CN110917112A

CN110917112A - Child nutritional shampoo prepared by protease enzymolysis

Info

Publication number: CN110917112A
Application number: CN201911404246.4A
Authority: CN
Inventors: 何智勇; 杨秋菱; 缪乐梅
Original assignee: FUJIAN MENGJIAOLAN DAILY CHEMICALS Co Ltd
Current assignee: FUJIAN MENGJIAOLAN DAILY CHEMICALS Co Ltd
Priority date: 2019-12-31
Filing date: 2019-12-31
Publication date: 2020-03-27

Abstract

The invention relates to the technical field of shampoo for children, and discloses a nutritional shampoo for children prepared by protease enzymolysis, which is prepared from the following raw materials in parts by weight: 50-120 parts of purified water, 10-20 parts of protein enzymatic hydrolysate, 5-15 parts of sugar-free black sesame paste, 3-12 parts of honey, 0.7-6 parts of glycerol, 0.5-3 parts of PEG-80 sorbitan laurate, 0.5-3 parts of cocamidopropyl betaine, 3-14 parts of cedar essential oil, 0.5-5 parts of polypeptide chelated calcium, 3-9 parts of olive leaf extract, 0.5-5 parts of enzymolysis raw material, 2-4 parts of chamomile extract, 0.5-1.5 parts of sodium laureth sulfate, 0.05-1 part of sodium benzoate, 0.05-0.5 part of sodium salicylate and 0.5-4 parts of lemon grass essential oil. The children nutritional shampoo prepared by protease enzymolysis is scientific in formula, mild and non-irritant, can reduce hair dryness and frizziness, is smooth and soft in hair, does not contain chemical additives, cannot cause damage to children hair and skin, and is more suitable for children.

Description

Child nutritional shampoo prepared by protease enzymolysis

Technical Field

The invention relates to the technical field of shampoo for children, in particular to children nutritional shampoo prepared by protease enzymolysis.

Background

The hair is hair growing on the head, the hair is mainly used for protecting the head, the hair is soft and fluffy and has elasticity, the hair can resist light collision and can help the evaporation of sweat on the head, the shampoo is one of the most widely applied shampoo products and is one of the most commonly used hair cosmetics, is used for cleaning grease, sweat scale, cells peeled off from the scalp, foreign dust, microorganisms, residues and bad smell of a styling product and the like secreted by a human body and adhered to the scalp and the hair, and contains a plurality of components, and the comprehensive effect of the components can play a role in cleaning the scalp and the hair. The shampoo is an essential hair washing product in life, and the requirements of people on the shampoo are higher and higher along with the continuous improvement of living standard and the importance of keeping the scalp and the hair clean and beautiful.

The shampoo has the functions of removing dandruff, preventing alopecia and the like, and a specific shampoo needs to be prepared according to the age end of a user, the shampoo for children becomes a more concerned object, however, because the skin of children is quite sensitive (especially scalp), the children are quite sensitive to chemical components and perfume components, especially a chemically synthesized interface active agent, the hair follicle is easily damaged due to strong degreasing force, the healthy growth of hair is influenced, and the skin of babies can be burdened, so the nutritional shampoo for children prepared by protease enzymolysis is provided.

Disclosure of Invention

The invention provides a children nutritional shampoo prepared by protease enzymolysis, which has the advantages of reducing dryness and frizziness of hair, softening and smoothing hair, no chemical additive, no harm to the hair and skin of children, suitability for children, convenience for reducing the preparation cost of the children nutritional shampoo prepared by protease enzymolysis, and capability of solving the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: a children nutritional shampoo prepared by protease enzymolysis comprises the following raw materials in parts by weight: 50-120 parts of purified water, 10-20 parts of protein enzymatic hydrolysate, 5-15 parts of sugar-free black sesame paste, 3-12 parts of honey, 0.7-6 parts of glycerol, 0.5-3 parts of PEG-80 sorbitan laurate, 0.5-3 parts of cocamidopropyl betaine, 3-14 parts of cedar essential oil, 0.5-5 parts of polypeptide chelated calcium, 3-9 parts of olive leaf extract, 0.5-5 parts of enzymolysis raw material, 2-4 parts of chamomile extract, 0.5-1.5 parts of sodium laureth sulfate, 0.05-1 part of sodium benzoate, 0.05-0.5 part of sodium salicylate and 0.5-4 parts of lemon grass essential oil.

Optionally, the preparation method of the polypeptide chelated calcium comprises the following steps: (1) weighing 1000g of fresh waste egg shells, cleaning, removing eggshell membranes, drying in an air drying oven at 60 ℃ for 3h, crushing by a 200-mesh crusher to obtain eggshell powder for later use, weighing 2.0g of eggshell powder, dissolving in 10-14mol/L hydrochloric acid, stirring at 80-95 ℃ for 60-120 min, naturally cooling to 25-35 ℃, filtering, and obtaining filtrate which is soluble calcium extract and can obtain 400.0mg of soluble calcium; (2) adding 10-16g of protein into 400g of 100-400g of water, adding 0.2-2g of flavourzyme and 0.5-5g of alkaline protease, carrying out enzymolysis for 5-14 hours at the temperature of 40-80 ℃, and inactivating enzyme to obtain a protease hydrolysate; (3) adding 75-150g of protein enzymolysis liquid into 50-100g of soluble calcium extracting solution, stirring for 30-120 minutes at the temperature of 30-45 ℃, adding 500-900g of ethanol water solution with the mass fraction of 95% to generate precipitate, filtering, and drying the precipitate in vacuum to obtain the polypeptide chelated calcium.

Optionally, the protein enzymatic hydrolysate, sugar-free black sesame paste, cocamidopropyl betaine, sodium laureth sulfate, PEG-80 sorbitan laurate, glycerol, honey, polypeptide chelated calcium, and cedar essential oil are stirred at a temperature of 70-90 ℃ and a rotation speed of 200-500 rpm for 2-4 hours, cooled to 50-60 ℃, added with cocamidopropyl betaine, sodium benzoate, sodium salicylate, olive leaf extract, and chamomile extract, stirred at a temperature of 45-50 ℃ and a rotation speed of 50-150 rpm for 30-60 minutes, cooled to 35-40 ℃, added with calcium extract, yeast extract, and lemongrass essential oil, heated, and stirred at a constant speed.

Optionally, the polypeptide chelated calcium can be prepared by taking a certain amount of wall-broken yeast powder, adding a certain amount of papain to hydrolyze at a pH of 6.0 and a temperature of 55 ℃ for a period of time, rapidly heating the enzymolysis solution to 100 ℃, maintaining for about 15min and inactivating enzymes, cooling to room temperature, adding an isovolumetric alginic acid solution into the crude yeast polypeptide extract, stirring with an electric stirrer at a rotation speed of 1000r/min for 0.6h, then washing with 0.6 times by volume of 0.4mol/L of hydrochloric acid to remove adsorbed polypeptides, suction filtering, repeating for 2-3 times, adding excessive sodium chloride into the eluate, stirring gently to reach a saturated state, standing in a refrigerator at a temperature of 2 ℃ for overnight, collecting yeast polypeptide precipitates, placing in the refrigerator for freezing storage, finely taking a certain amount of eggshell acidolysis solution, adjusting the pH, mixing with the yeast polypeptide solution according to a certain volume ratio, adding 20mL of absolute ethyl alcohol at the same temperature, shaking at 6000r/min for 15min, and taking supernatant to determine the content of free calcium.

Optionally, cleaning the enzymolysis raw material, drying, crushing, sieving with a 200-mesh sieve, taking 10-30g of powder, adding into a hydrochloric acid solution with the concentration of 6-8mol/L and the volume of 25-100mL, adding 80-150g of water, stirring at the temperature of 80-95 ℃ for 60-120 minutes, naturally cooling to 25-35 ℃, and filtering to obtain a filtrate which is a soluble calcium extracting solution; adding 8.5-15g of protein into 400g of 100-400g of water, adding 0.2-2g of flavourzyme and 0.5-5g of alkaline protease, carrying out enzymolysis for 4-12 hours at the temperature of 40-60 ℃, and inactivating enzyme to obtain a protease hydrolysate; adding 75-150g of protein enzymolysis liquid into 50-100g of soluble calcium extracting solution, stirring for 30-120 minutes at the temperature of 30-45 ℃, adding 300-800g of ethanol water solution with the mass fraction of 95%, and filtering to obtain the soluble calcium extracting solution.

Optionally, the preparation method of the protein enzymolysis solution comprises the following steps: (1) selecting 50g of rice with full and transparent grains, cleaning, drying and grinding into rice flour; (2) the pH is then adjusted to 8, followed by addition of proteases which increase the solubility of rice proteins, such as: hydrolyzing rice protein with complex enzyme, weighing a certain amount of rice protein, placing into an enzyme reactor according to a solid-to-liquid ratio of 1:10, adjusting the pH value to the optimum value of enzyme reaction, adding a certain amount of enzyme, and timing; (3) the extraction regulation is as follows: inactivating enzyme at 100 deg.C for 15min, and centrifuging at 3000r/min for 20 min.

The beneficial effects are as follows:

1. the children nutritional shampoo prepared by protease enzymolysis uses the chamomile extract, the olive leaf extract and the lemon grass essential oil, the chamomile extract can balance grease secretion, inhibit grease and resist acne, the problem that hair of many children is dry and hard due to poor scalp hygiene or heredity is effectively solved, hair is helped to become glossier and softer, the olive leaf extract has various pharmacological actions such as antibiosis, antivirus, antimicrobial and anticancer, the lemon grass essential oil has good cleaning and deodorizing effects, hair follicles can be strengthened, stimulated scalp can be relieved, dandruff can be reduced, the formula is scientific, mild and non-irritant, the chamomile extract, the olive leaf extract and the lemon grass essential oil are added, the effects of water and oil balance, nourishing, softening and protecting are good, hair dryness and frizziness can be reduced after the shampoo is used, hair is soft and smooth, no chemical additive, no harm to children hair and skin, and is more suitable for children.

2. According to the children nutritional shampoo prepared by protease enzymolysis, fresh waste egg shells are cleaned, egg shell membranes are removed, calcium ions are extracted by crushing and acid hydrolysis, and polypeptide chelated calcium substances in the shampoo are prepared according to the volume ratio of yeast polypeptide to egg shell acidolysis solution of 15:1, so that the waste egg shells in life can be reused, and the preparation cost of the children nutritional shampoo prepared by protease enzymolysis can be reduced.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

A children nutritional shampoo prepared by protease enzymolysis is prepared from the following raw materials in parts by weight: 50-120 parts of purified water, 10-20 parts of protein enzymatic hydrolysate, 5-15 parts of sugar-free black sesame paste, 3-12 parts of honey, 0.7-6 parts of glycerol, 0.5-3 parts of PEG-80 sorbitan laurate, 0.5-3 parts of cocamidopropyl betaine, 3-14 parts of cedar essential oil, 0.5-5 parts of polypeptide chelated calcium, 3-9 parts of olive leaf extract, 0.5-5 parts of enzymolysis raw materials, 2-4 parts of chamomile extract, 0.5-1.5 parts of sodium laureth sulfate, 0.05-1 part of sodium benzoate, 0.05-0.5 part of sodium salicylate, 0.5-4 parts of lemon grass essential oil and lemon grass essential oil have good cleaning and deodorizing effects, can strengthen hair follicles, relieve irritated scalp and are favorable for reducing dandruff, and the formula is scientific, mild and non-irritant.

The preparation method of the polypeptide chelated calcium comprises the following steps: (1) weighing 1000g of fresh waste egg shells, cleaning, removing eggshell membranes, drying in an air drying oven at 60 ℃ for 3h, crushing by a 200-mesh crusher to obtain eggshell powder for later use, weighing 2.0g of eggshell powder, dissolving in 10-14mol/L hydrochloric acid, stirring at 80-95 ℃ for 60-120 min, naturally cooling to 25-35 ℃, filtering, and obtaining filtrate which is soluble calcium extract and can obtain 400.0mg of soluble calcium; (2) adding 10-16g of protein into 400g of 100-400g of water, adding 0.2-2g of flavourzyme and 0.5-5g of alkaline protease, carrying out enzymolysis for 5-14 hours at the temperature of 40-80 ℃, and inactivating enzyme to obtain a protease hydrolysate; (3) adding 75-150g of protein enzymolysis liquid into 50-100g of soluble calcium extracting solution, stirring for 30-120 minutes at the temperature of 30-45 ℃, adding 500-900g of ethanol aqueous solution with the mass fraction of 95% to generate precipitation, filtering, drying the precipitation in vacuum to obtain polypeptide chelated calcium, cleaning fresh and waste egg shells, removing eggshell membranes, crushing and carrying out acid hydrolysis to extract calcium ions, preparing the polypeptide chelated calcium substance in the shampoo by using the yeast polypeptide and the eggshell acidolysis liquid with the volume ratio of 15:1, facilitating the reutilization of the waste egg shells in life, and facilitating the reduction of the preparation cost of the children nutritional shampoo prepared by using protease enzymolysis.

Wherein, the protein enzymatic hydrolysate, sugar-free black sesame paste, cocamidopropyl betaine, sodium laureth sulfate, PEG-80 sorbitan laurate, glycerol, honey, polypeptide chelated calcium and cedar essential oil are stirred at the temperature of 70-90 ℃ and the rotating speed of 200-500 revolutions per minute for 2-4 hours, cooled to 50-60 ℃, added with cocamidopropyl betaine, sodium benzoate, sodium salicylate, olive leaf extract and chamomile extract, stirred at the temperature of 45-50 ℃ and the rotating speed of 50-150 revolutions per minute for 30-60 minutes, cooled to 35-40 ℃, added with calcium extract, yeast extract and lemongrass essential oil, heated and stirred at constant speed, the chamomile extract can balance grease secretion, inhibit grease and resist acne, effectively solving the problem of dry and hard hair of many children caused by poor sanitation or heredity of the scalp, helping the hair to become more shiny and soft.

Wherein the polypeptide chelated calcium can be prepared by taking a certain amount of wall-broken yeast powder, adding a certain amount of papain to hydrolyze at pH6.0 and temperature of 55 ℃ for a period of time, rapidly heating the enzymolysis solution to 100 ℃, maintaining for about 15min and inactivating enzyme, cooling to room temperature, adding an isovolumetric alginic acid solution into the crude yeast polypeptide extract, stirring with an electric stirrer at a rotation speed of 1000r/min for 0.6h, then washing with 0.6 times of hydrochloric acid with a volume of 0.4mol/L to remove adsorbed polypeptide, performing suction filtration, repeating for 2-3 times, adding excessive sodium chloride into the eluate, stirring gently to reach a saturated state, standing in a refrigerator at 2 ℃ for overnight, collecting yeast polypeptide precipitate, placing in the refrigerator for freeze preservation, finely taking a certain amount of eggshell acidolysis solution, adjusting pH, mixing with the yeast polypeptide solution according to a certain proportional volume, adding 20mL of absolute ethyl alcohol at the same temperature, shaking, rotating at 6000r/min for 15min, taking supernatant, measuring the content of free calcium, and respectively injecting the mixture according to the volume ratio of (10:1, 15:1 and 20:1), the pH value of (6.5, 7.0 and 7.5), the temperature of (30, 40 and 50) and the time of (30, 40 and 50), wherein the optimal volume ratio is 15:1 as shown in Table 1.

TABLE 1 optimal integration Table for Yeast polypeptide to calcium ion volume ratio

Wherein, the enzymolysis raw material is cleaned, dried, crushed and sieved by a 200-mesh sieve, 10-30g of powder is taken and added into hydrochloric acid solution with the concentration of 6-8mol/L and the volume of 25-100mL, 80-150g of water is added, the mixture is stirred for 60-120 minutes at the temperature of 80-95 ℃, and is naturally cooled to 25-35 ℃ and then filtered, and the obtained filtrate is soluble calcium extract; adding 8.5-15g of protein into 400g of 100-400g of water, adding 0.2-2g of flavourzyme and 0.5-5g of alkaline protease, carrying out enzymolysis for 4-12 hours at the temperature of 40-60 ℃, and inactivating enzyme to obtain a protease hydrolysate; adding 75-150g of protein enzymatic hydrolysate into 50-100g of soluble calcium extracting solution, stirring for 30-120 minutes at the temperature of 30-45 ℃, adding 800g of ethanol aqueous solution with the mass fraction of 95% and filtering to obtain the soluble calcium extracting solution, and adding chamomile extract, olive leaf extract and lemon grass essential oil, so that the water-oil balance, nourishing and softening protection effects are good, dry and frigid hair can be reduced after the hair-nourishing hair-softening hair.

The preparation method of the protein enzymolysis liquid comprises the following steps: (1) selecting 50g of rice with full and transparent grains, cleaning, drying and grinding into rice flour; (2) the pH is then adjusted to 8, followed by addition of proteases which increase the solubility of rice proteins, such as: hydrolyzing rice protein with complex enzyme, weighing a certain amount of rice protein, placing into an enzyme reactor according to a solid-liquid ratio of 1:10 (the solid-liquid ratio refers to the mass or volume ratio of solid phase and liquid phase in suspension) to adjust the pH value to the optimum value of enzyme reaction, adding quantitative enzyme, and timing; (3) the extraction regulation is as follows: inactivating enzyme at 100 deg.C for 15min, centrifuging at 3000r/min for 20min, and preparing shampoo component with rice to save shampoo cost.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A children nutritional shampoo prepared by protease enzymolysis is characterized in that: the composition is prepared from the following raw materials in parts by weight: 50-120 parts of purified water, 10-20 parts of protein enzymatic hydrolysate, 5-15 parts of sugar-free black sesame paste, 3-12 parts of honey, 0.7-6 parts of glycerol, 0.5-3 parts of PEG-80 sorbitan laurate, 0.5-3 parts of cocamidopropyl betaine, 3-14 parts of cedar essential oil, 0.5-5 parts of polypeptide chelated calcium, 3-9 parts of olive leaf extract, 0.5-5 parts of enzymolysis raw material, 2-4 parts of chamomile extract, 0.5-1.5 parts of sodium laureth sulfate, 0.05-1 part of sodium benzoate, 0.05-0.5 part of sodium salicylate and 0.5-4 parts of lemon grass essential oil.

2. The children nutritional shampoo prepared by protease enzymolysis according to claim 1, wherein the shampoo comprises: the preparation method of the polypeptide chelated calcium comprises the following steps: (1) weighing 1000g of fresh waste egg shells, cleaning, removing eggshell membranes, drying in an air drying oven at 60 ℃ for 3h, crushing by a 200-mesh crusher to obtain eggshell powder for later use, weighing 2.0g of eggshell powder, dissolving in 10-14mol/L hydrochloric acid, stirring at 80-95 ℃ for 60-120 min, naturally cooling to 25-35 ℃, filtering, and obtaining filtrate which is soluble calcium extract and can obtain 400.0mg of soluble calcium; (2) adding 10-16g of protein into 400g of 100-400g of water, adding 0.2-2g of flavourzyme and 0.5-5g of alkaline protease, carrying out enzymolysis for 5-14 hours at the temperature of 40-80 ℃, and inactivating enzyme to obtain a protease hydrolysate; (3) adding 75-150g of protein enzymolysis liquid into 50-100g of soluble calcium extracting solution, stirring for 30-120 minutes at the temperature of 30-45 ℃, adding 500-900g of ethanol water solution with the mass fraction of 95% to generate precipitate, filtering, and drying the precipitate in vacuum to obtain the polypeptide chelated calcium.

3. The children nutritional shampoo prepared by protease enzymolysis according to claim 1, wherein the shampoo comprises: the protein enzymatic hydrolysate, sugar-free black sesame paste, cocamidopropyl betaine, sodium laureth sulfate, PEG-80 sorbitan laurate, glycerol, honey, polypeptide chelated calcium and cedar essential oil are stirred at the temperature of 70-90 ℃ and the rotating speed of 200-500 revolutions/minute for 2-4 hours, cooled to 50-60 ℃, added with cocamidopropyl betaine, sodium benzoate, sodium salicylate, olive leaf extract and chamomile extract, stirred at the temperature of 45-50 ℃ and the rotating speed of 50-150 revolutions/minute for 30-60 minutes, cooled to 35-40 ℃, added with calcium extract, yeast extract and lemongrass essential oil, heated and stirred at a constant speed.

4. The children nutritional shampoo prepared by protease enzymolysis according to claim 1, wherein the shampoo comprises: the polypeptide chelated calcium can be prepared by taking a certain amount of wall-broken yeast powder, adding a certain amount of papain to hydrolyze at a pH value of 6.0 and a temperature of 55 ℃ for a period of time, quickly heating the enzymolysis solution to 100 ℃, maintaining for about 15min and inactivating enzyme, cooling to room temperature, adding an isovolumetric alginic acid solution into the crude yeast polypeptide extract, stirring for 0.6h under a condition of a rotation speed of 1000r/min by using an electric stirrer, then washing with 0.6 times of hydrochloric acid with a volume of 0.4mol/L to remove adsorbed polypeptide, performing suction filtration, repeating for 2-3 times, adding excessive sodium chloride into the eluate, stirring gently to reach a saturated state, standing overnight in a refrigerator at a temperature of 2 ℃, collecting yeast polypeptide precipitate, placing in the refrigerator for freezing storage, finely taking a certain amount of eggshell acidolysis solution, adjusting pH, mixing with the yeast polypeptide solution according to a certain volume ratio, adding 20mL of absolute ethyl alcohol at the same temperature, shaking at 6000r/min for 15min, and taking supernatant to determine the content of free calcium.

5. The children nutritional shampoo prepared by protease enzymolysis according to claim 1, wherein the shampoo comprises: cleaning an enzymolysis raw material, drying, crushing, sieving with a 200-mesh sieve, taking 10-30g of powder, adding into a hydrochloric acid solution with the concentration of 6-8mol/L and the volume of 25-100mL, adding 80-150g of water, stirring at the temperature of 80-95 ℃ for 60-120 minutes, naturally cooling to 25-35 ℃, and filtering to obtain a filtrate which is a soluble calcium extracting solution; adding 8.5-15g of protein into 400g of 100-400g of water, adding 0.2-2g of flavourzyme and 0.5-5g of alkaline protease, carrying out enzymolysis for 4-12 hours at the temperature of 40-60 ℃, and inactivating enzyme to obtain a protease hydrolysate; adding 75-150g of protein enzymolysis liquid into 50-100g of soluble calcium extracting solution, stirring for 30-120 minutes at the temperature of 30-45 ℃, adding 300-800g of ethanol water solution with the mass fraction of 95%, and filtering to obtain the soluble calcium extracting solution.

6. The children nutritional shampoo prepared by protease enzymolysis according to claim 1, wherein the shampoo comprises: the preparation method of the protein enzymolysis liquid comprises the following steps: (1) selecting 50g of rice with full and transparent grains, cleaning, drying and grinding into rice flour; (2) the pH is then adjusted to 8, followed by addition of proteases which increase the solubility of rice proteins, such as: hydrolyzing rice protein with complex enzyme, weighing a certain amount of rice protein, placing into an enzyme reactor according to a solid-to-liquid ratio of 1:10, adjusting the pH value to the optimum value of enzyme reaction, adding a certain amount of enzyme, and timing; (3) the extraction regulation is as follows: inactivating enzyme at 100 deg.C for 15min, and centrifuging at 3000r/min for 20 min.