CN102525866B - Cosmetic additive, and preparation method and application thereof - Google Patents
Cosmetic additive, and preparation method and application thereof Download PDFInfo
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- CN102525866B CN102525866B CN 201210007434 CN201210007434A CN102525866B CN 102525866 B CN102525866 B CN 102525866B CN 201210007434 CN201210007434 CN 201210007434 CN 201210007434 A CN201210007434 A CN 201210007434A CN 102525866 B CN102525866 B CN 102525866B
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Abstract
The invention discloses a cosmetic additive, which consists of the following components in percentage by weight: 50 to 70 percent of earthworm active protein extract and 30 to 50 percent of sea cucumber peptide extract. The cosmetic additive has the effects of making skin remarkably exquisite and tender, remarkably enhancing elasticity, remarkably smoothening wrinkles, increasing the moisture content of the skin, and the like, can be directly used for the skin as moisturizing essence, and can also be added into lotion, essence, milk or cream to prepare anti-wrinkle repair cosmetic, wherein the amount of the cosmetic additive is 0.5 to 10 percent of the total weight of the anti-wrinkle repair cosmetic in actual application. The cosmetic additive has the advantages of high activity, high biocompatibility, safety, no side effect and the like.
Description
Technical field
The present invention relates to a kind of cosmetics additive and preparation method thereof and application.
Background technology
Skin aging is a kind of gradual physiological process, direct cutaneous outward appearance and function of continuing.Wrinkle then is a kind of sign of skin aging performance, is referred to as " annual ring of life ".Along with people's raising of quality of life, anti-aging, remove wrinkle, return to the topic that the youth has become consumers in general to pay close attention to.
At present, along with the development of biochemical technology, people open the physiological mechanism of wrinkle just gradually, and it is increasing to use biological active substances to improve the probability that skin wrinkle occurs because relaxing, centered by cosmetics producer, allow the tip research of perpetual youth on the face especially noticeable in recent years.Thereby anti-aging anti-wrinkle cosmetic is more and more paid attention to by people.
Skin aging is the part of body aging, and with age, following outward manifestation can appear in skin: cutis laxa, attenuation, and the water content of skin descends, and often seems dry, and squama is arranged, and tarnishes; Elasticity and consolidation also descend thereupon; Wrinkle forms, and increases gradually, deepens.In addition, the sensitivity of skin increases, and pigment also increases.Hyperpigmentation is mainly seen in the sun exposure position, as face, the back of the hand and forearm.The skin aging inherence shows as the skin cell proliferation ability and descends, and epidermal renewal speed slows down, and the epidermal renewal ability weakens, and dermal collagen is synthetic to slow down, and skin immunization power descends, pigment skewness etc.
In the prior art, form principle and process at wrinkle, the anti-aging crease-resistant active component of having developed mainly comprises: influence the composition of histiocyte growth metabolism, as cell growth factor, some animals and plants extract, fruit acid class material, sheep placental extract etc.; Promote skin moisture-keeping and the active component of repairing skin barrier function, as ceramide, hyaluronic acid, pyrrolidone sodium carboxylate etc.; The composition of anti-oxidative damage is as superoxide dismutase, glutathione peroxidase, vitamin E and vitamin C, coenzyme Q10 etc.Have not yet to see the report that earthworm active protein and Stichopus japonicus peptide have anti-aging function such as crease-resistant.
Summary of the invention
At above-mentioned prior art, the invention provides a kind of cosmetics additive, and its preparation method be provided, with and repair application in the cosmetics in the preparation wrinkle removal.
The present invention is achieved by the following technical solutions:
A kind of cosmetics additive is made up of earthworm active protein extract and Stichopus japonicus peptide extract, and wherein, the earthworm active protein extract accounts for 50～70%, and the Stichopus japonicus peptide extract accounts for 30～50%, by weight percentage.
Described earthworm active protein extract prepares by the following method: the fresh and alive Lumbricus that will clean up and the phosphate buffer of pH6.0～8.5 are with mass ratio 1: the ratio of (1～10) is mixed, after organizing the inscribe refiner to pulverize homogenate, leave standstill 1～10h, make the abundant stripping of albumen; Then with homogenate with 2000～8000rpm high speed centrifugation, 5～30min, discard precipitation, add ammonium sulfate saltout (so that the protein component precipitation is separated out) in the supernatant, reach 60% saturation to ammonium sulfate, add the back and continue to stir 10min ammonium sulfate is fully dissolved, then with solution with 2000～8000rpm high speed centrifugation, 5～30min, discard precipitation, supernatant adopts the bag filter dialysis desalination of 3000 molecular weight, namely gets the earthworm active protein extract after lyophilization or the spray drying.
Described Stichopus japonicus peptide extract prepares by the following method: after bright Stichopus japonicus is cleaned chopping, carry out enzymolysis with pepsin and two kinds of enzymes of trypsin: at first use the pepsin enzymolysis, pH is 2.0～2.5,35～60 ℃ of hydrolysis 2～8h, use the Trypsin enzymolysis then, pH is 8.0～8.5,35～60 ℃ of hydrolysis 2～8h, precipitation with alcohol (the ethanol volume ratio is 40～80%) is got supernatant, namely gets the Stichopus japonicus peptide extract after lyophilization or the spray drying.
The preparation method that described cosmetics add is: earthworm active protein extract and Stichopus japonicus peptide extract are proportionally mixed, and mixing namely gets cosmetics additive.The preparation method of earthworm active protein extract and Stichopus japonicus peptide extract such as above-mentioned.
Described cosmetics additive have the skin of making obviously delicate, elasticity obviously increases, effects such as wrinkle obviously shoals, moisture content of skin increase, can be used as the elite of preserving moisture, be directly used in skin, also can add in surfactant, elite, emulsion or the facial cream, make wrinkle removal and repair cosmetics, during practical application, cosmetics additive accounts for wrinkle removal and repairs 0.5～10% of cosmetics gross weight.
Cosmetics additive of the present invention can be used as elite and is directly used in skin, reaches the effect of crease-resistant reparation, also can add in surfactant, elite, emulsion or the facial cream, reaches the effect of crease-resistant reparation.Cosmetics additive of the present invention has active height, good biocompatibility, safe advantage such as have no side effect.
The specific embodiment
The present invention is further illustrated below in conjunction with embodiment.
Embodiment 1: the extraction of earthworm active protein extract
The fresh and alive Lumbricus 500g that cleans up is mixed with 1: 2 ratio of mass ratio with the phosphate buffer of pH7.2, and with organizing the inscribe refiner to pulverize homogenate, the Lumbricus homogenate is left standstill 6h, make the abundant stripping of albumen.Homogenate with 7000rpm high speed centrifugation 15min, is discarded precipitation, and supernatant adds ammonium sulfate and saltouts to reach 60% saturation, and the protein component precipitation is separated out.Adding back continuation stirring 10min fully dissolves ammonium sulfate.Solution with 4000rpm high speed centrifugation 15min, is discarded precipitation.Supernatant adopts the bag filter dialysis desalination of 3000 molecular weight, and lyophilization namely gets earthworm active protein extract 3g.
Embodiment 2: the extraction of Stichopus japonicus peptide extract
After bright Stichopus japonicus 500g cleans chopping, carry out enzymolysis with pepsin and two kinds of enzymes of trypsin: be 2.0-2.5 with pepsin enzymolysis pH at first, 50 ℃ of hydrolysis 5h, be 8.0-8.5 with Trypsin enzymolysis pH then, 50 ℃ of hydrolysis 5h, precipitation with alcohol (the ethanol volume ratio is 60%) is got supernatant, and lyophilization namely gets Stichopus japonicus peptide extract 50g.Pepsin and tryptic addition get final product according to the routine interpolation.
Embodiment 3: the preparation wrinkle removal is repaired astringent
Prescription is:
Add MEIGUI CHUNLU to 100ml.
The compound method of these cosmetics is as follows: the MEIGUI CHUNLU that adds 80ml behind earthworm active protein extract (embodiment 1 preparation), Stichopus japonicus peptide extract (embodiment 2 preparations), trehalose, the hyaluronic acid powder mixing is stirred, leaving standstill 12-24h dissolves fully to above-mentioned composition, add glycerol and germall Bp, add MEIGUI CHUNLU to 100ml.
Embodiment 4: wrinkle removal is repaired the use sense evaluation of astringent among the embodiment 3
Wrinkle removal for preparation among the embodiment 3 is repaired astringent, and the 2ml cosmetics are evenly coated 100 volunteers (at 30 years old to 50 years old volunteer's age, wherein male volunteer is 50,50 of women volunteers at every turn.Face has microgroove, some position wrinkle is obvious) face, do contrast with the astringent (consumption of all the other components is with embodiment 3) that does not add earthworm active protein extract and Stichopus japonicus peptide extract, used continuously 28 days.It uses sense, skin texture situation post-evaluation in 28 days.
Evaluation criterion: use sense by volunteer's readme impression; The skin texture situation is observed and the skin texture experiment by naked eyes are directly perceived, wherein, effectively is: skin texture obviously attenuate greasy, perusal face microgroove obviously reduces, stricture of vagina shoals deeply; Be generally: skin texture shoals slightly, perusal reduces than the preceding wrinkle of experiment, but reduces not obvious; Invalid: skin texture and the preceding no any variation of experiment.
The results are shown in Table 1:
Table 1 wrinkle removal is repaired astringent table on probation
As seen from the above table, wrinkle removal is repaired astringent and is used after 30 days, and most people use sense good, have tangible wrinkle removal repairing effect, and are especially remarkably productive with wrinkle removal.
Claims (5)
1. cosmetics additive, it is characterized in that: be made up of earthworm active protein extract and Stichopus japonicus peptide extract, wherein, the earthworm active protein extract accounts for 50～70%, and the Stichopus japonicus peptide extract accounts for 30～50%, by weight percentage;
Described earthworm active protein extract prepares by the following method: the fresh and alive Lumbricus that will clean up and the phosphate buffer of pH6.0～8.5 are with mass ratio 1:(1～10) ratio mix, after pulverizing homogenate, leave standstill 1～10h, then with homogenate with the centrifugal 5～30min of 2000～8000rpm, discard precipitation, adding ammonium sulfate in the supernatant saltouts, reach 60% saturation to ammonium sulfate, stirring makes the ammonium sulfate dissolving, then with solution with the centrifugal 5～30min of 2000～8000rpm, discard precipitation, supernatant adopts the bag filter dialysis desalination of 3000 molecular weight, namely gets the earthworm active protein extract after lyophilization or the spray drying;
Described Stichopus japonicus peptide extract prepares by the following method: after bright Stichopus japonicus is cleaned chopping, carry out enzymolysis with pepsin and two kinds of enzymes of trypsin: at first use the pepsin enzymolysis, pH is 2.0～2.5,35～60 ℃ of hydrolysis 2～8h, use the Trypsin enzymolysis then, pH is 8.0～8.5,35～60 ℃ of hydrolysis 2～8h, precipitation with alcohol is got supernatant, namely gets the Stichopus japonicus peptide extract after lyophilization or the spray drying.
2. the preparation method of the described cosmetics additive of claim 1, it is characterized in that: step is as follows:
(1) preparation earthworm active protein extract: the fresh and alive Lumbricus that will clean up and the phosphate buffer of pH6.0～8.5 are with mass ratio 1:(1～10) ratio mix, after pulverizing homogenate, leave standstill 1～10h, then with homogenate with the centrifugal 5～30min of 2000～8000rpm, discard precipitation, adding ammonium sulfate in the supernatant saltouts, reach 60% saturation to ammonium sulfate, stirring makes the ammonium sulfate dissolving, then with solution with the centrifugal 5～30min of 2000～8000rpm, discard precipitation, supernatant adopts the bag filter dialysis desalination of 3000 molecular weight, namely gets the earthworm active protein extract after lyophilization or the spray drying;
(2) preparation Stichopus japonicus peptide extract: after bright Stichopus japonicus is cleaned chopping, carry out enzymolysis with pepsin and two kinds of enzymes of trypsin: at first use the pepsin enzymolysis, pH is 2.0～2.5,35～60 ℃ of hydrolysis 2～8h, use the Trypsin enzymolysis then, pH is 8.0～8.5,35～60 ℃ of hydrolysis 2～8h, precipitation with alcohol is got supernatant, namely gets the Stichopus japonicus peptide extract after lyophilization or the spray drying;
(3) mix: earthworm active protein extract and Stichopus japonicus peptide extract are proportionally mixed, and mixing namely gets cosmetics additive.
3. the application of the described cosmetics additive of claim 1 in preparation wrinkle removal reparation cosmetics.
4. application according to claim 3 is characterized in that: described cosmetics additive accounts for wrinkle removal and repairs 0.5～10% of cosmetics gross weight.
5. application according to claim 3 is characterized in that: it is surfactant, elite, emulsion or facial cream that described wrinkle removal is repaired cosmetics.
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885751B (en) * | 2012-10-30 | 2014-07-02 | 王玉芝 | Making-up paste with wrinkle repairing function |
| CN104887541A (en) * | 2015-02-27 | 2015-09-09 | 济宁华能制药厂有限公司 | Cosmetic containing earthworm active protein |
| CN104725466A (en) * | 2015-02-27 | 2015-06-24 | 济宁华能制药厂有限公司 | Method for extracting earthworm active protein for cosmetics |
| CN105997829A (en) * | 2016-07-22 | 2016-10-12 | 杨凌三度蚯蚓科技有限公司 | Earthworm extract for skin-nourishing cosmetics and preparation method and application thereof |
| CN107513881A (en) * | 2017-08-30 | 2017-12-26 | 山东秦世集团有限公司 | A kind of antibacterial archival paper and preparation method thereof |
| CN107737078A (en) * | 2017-09-13 | 2018-02-27 | 山东圣洲海洋生物科技股份有限公司 | A kind of sea cucumber Essence |
| CN107927764A (en) * | 2017-11-20 | 2018-04-20 | 黑龙江美丽石岛科技开发有限公司 | A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof |
| CN111991281B (en) * | 2019-10-15 | 2022-07-19 | 时垠(上海)生物科技有限公司 | Preparation method of natural anti-wrinkle cosmetic material |
| CN110787080A (en) * | 2019-11-04 | 2020-02-14 | 江西沐恩堂生物科技有限公司 | Earthworm anti-wrinkle composition and application thereof |
| CN114934090B (en) * | 2022-06-29 | 2023-11-24 | 北海黑珍珠海洋生物科技有限公司 | Sea cucumber peptide with moisturizing effect and preparation method thereof |
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| CN101514354A (en) * | 2009-02-25 | 2009-08-26 | 山东大学 | Sea cucumber polypeptide, preparation method and application thereof |
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Application publication date: 20120704 Assignee: Guangzhou Asiana Biological Technology Co., Ltd. Assignor: Shandong University ai Weihai Contract record no.: 2014440000102 Denomination of invention: Cosmetic additive, and preparation method and application thereof Granted publication date: 20130710 License type: Exclusive License Record date: 20140314 |
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