KR20020065433A - The method of preparing water-soluble pearl powder using acid hydrolysis in normal temperature - Google Patents

Abstract

PURPOSE: A water soluble pearl powder using acid hydrolysis in normal temperature is provided, which has improved absorption of calcium into human body and fortifies immune system. CONSTITUTION: The preparation method of water soluble pearl powder includes the steps of pulverizing pearl into 500 to 1000 mesh size; adding two or three times of water to the pearl powder followed by filtering; reacting 1-20 vol.% of glacial acetic acid with solids obtained in a second step at 350 to 600 rpm until CO2 gas is not generated any more; filtering above solution to separate solid fraction from liquid fraction; rinsing the solid fraction with distilled water to remove residual aqueous soluble components; spray drying liquid fraction to prepare water soluble pearl calcium and protein powder; and mixing obtained pearl powder with mushroom extracts, silk amino acid, and vitamin D.

Description

The method of preparing water-soluble pearl powder using acid hydrolysis in normal temperature}

The present invention relates to a method for preparing water-soluble pearl calcium using room temperature acid hydrolysis, and more particularly, to a series of treatment processes such as calcium salt produced by the reaction of calcium carbonate and an acidic substance of pearl and a small amount of hydrolyzed water-soluble protein. It is a kind of pure white-white powder obtained from. Its active ingredient is water-soluble calcium salt, conchiolin protein (containing several essential amino acids), trace elements as cosmetics, creams and ointments with anti-inflammatory and skin protection, health food and various The present invention relates to a method for producing a water-soluble pearl powder that can be used as a raw material for adding food.

In general, pearls are a solid substance produced in the shellfish, and the main component is CaCo ₃ 91.7% calcium carbonate, 5.7% other organic matter and 2.2% moisture. (conchiolin) occupies. Conchiolin is a natural protein consisting of 7 to 14 amino acids and is a natural binding substance that forms calcium in the form of pearls by binding calcium carbonate, which is a major component of pearls, to each other.

In addition, pearls have been used as an herbal medicine in ancient times, and according to the Korean Medicine Dictionary, "Pearls are sweet, salty, and cold. And boils well. Pediatric sensation (驚風), palpitations, sleep disorders, yilong (耳聾), small brown disease (간), liver fever, and redness of the eyes are good to tingle. " It is written. As such, pearls, which have been used in various folk medicines, are calcium and conchiolin which exhibit physiological activities, and inorganic ingredients that contain small amounts. Calcium, when ingested, strengthens bones, lowers blood pressure, promotes digestion, and is also said to be healthy. When applied to face and skin, it can also be used as a burn remedy or hair growth promoter.

The conchiolin is known to have an effect of improving blood circulation disorders and to prevent skin aging when ingested as an amino acid complex. In Japan and China, pearl powders sintered at high temperatures are commercialized, but in this case, only calcium and inorganic components are effective among the active ingredients of pearls, and all protein components are lost. Can not expect

Pearl is a high calcium cultured only in uncontaminated natural conditions, and can be differentiated from other calcium products that are suspected of safety, such as shell calcium, which may cause heavy metal contamination, and cow bone powder, which may cause mad cow disease. It was a matter of fact.

Conventional methods for preparing water-soluble pearl powders are classified into physical (heating) method, chemical (acid alkali method) and biochemical (enzyme method) according to methods of hydrolyzing calcium carbonate and insoluble protein components, which are the main components of pearls. .

Preparation Example 1 Physical Method (Heating Press Method)

The pearl powder is added to an aqueous solution, sealed, and reacted for 10 minutes to 1 hour at a temperature of 90 to 150 and a pressure of 50 to 150 kPa to decolorize and dehydrate the water-soluble protein eluted in the aqueous solution. Although the yield per unit volume of the aqueous solution to react is high, the absolute volume of the aqueous solution to be treated is small, so that the overall productivity is very low.

Preparation Example 2 Chemical Method (Acid Alkaline Hydrolysis)

Remove the carbonate by adding concentrated acid or dilute alkaline aqueous solution to the pearl powder, and then wash filtration, hydrolysis, neutralization, decolorization, desalting, or hydrolyze, neutralize, and filter protein components in dilute alkaline and alcoholic solutions. You must go through the process.

Preparation Example 3 Biochemical Method (Enzyme Method)

Calcium carbonate is removed and the remaining insoluble matter is reacted with proteolytic enzymes to elute it with an aqueous solution. The enzyme is inactivated and then filtered and dried. Compared with other methods, the reaction conditions are milder, the auxiliary facilities are simpler, and the yields are comparable with chemical methods, but the enzyme is expensive and the complete hydrolysis is difficult. In addition, there is a problem such as increased turbidity of the aqueous solution due to the remaining enzyme protein after the reaction is completed.

In the pearl powder prepared by the above preparation, calcium carbonate and protein, which are the main components of pearl, are hardly soluble in water and are difficult to be absorbed in the body because only a very small amount is dissolved in the gastrointestinal tract.

That is, calcium and protein contained in pearls are difficult to be absorbed into the body in the original state, and thus, despite the excellent physiological activity described above, it is difficult to obtain a desired effect. In order to maximize such physiological activity, attempts have been made to easily convert the useful components of pearls into water-soluble and absorb the body.

The research results reported so far have produced water-soluble pearl powder by selecting one or two or more of the above-mentioned physical, chemical and biochemical methods. It is a difficult situation to apply.

The present invention has been developed a method for producing a water-soluble material by dramatically reducing the energy consumption, a problem of the above-described process and taking the active ingredient of pearl in high yield in the same amount of pearl powder. In addition, the regularity of the solvent used in the acid hydrolysis process is established to achieve the uniformity of the overall process.

Accordingly, an object of the present invention is to provide a method for preparing a water-soluble pearl powder having a high content of calcium and active protein by using physical and chemical methods, by mixing mushroom polysaccharide extract, mucopolysaccharide protein, silk amino acid, vitamin D, etc. It is to manufacture functional water-soluble pearl powder that can increase absorption rate and induce immunity enhancement and skin aging prevention.

The method of the present invention for achieving the above object is pulverized pearl to 500 ~ 1,000 mesh particle size and then added to 5 ~ 20% (v / v) aqueous solution of edible glacial acetic acid 350 ~ 600rpm, until no carbon dioxide is generated, A first step of reacting at room temperature, stopping stirring and precipitating solids to separate the first liquid; A second step of separating residual components and insoluble solids two or three times by adding purified water having the same volume as the solids of the first step; A third step of mixing the first liquid and the second liquid to neutralize or filter and spray-dry to prepare a water-soluble pearl calcium; Soluble pearl calcium as it is or mixed with a mushroom polysaccharide extract, mucopolysaccharide protein, silk amino acid, vitamin D and the like is made to prepare a functional water-soluble pearl powder.

Pearls used in the method for producing water-soluble pearl calcium according to the present invention has a commodity value among sea pearls, freshwater pearls and natural pearls grown in the Chinese or Indonesian coastal waters due to their lack of domestic aquaculture. It is preferable to use an external product without the like.

The pearls are pulverized in a pulverizer until the particle size is 500 to 1,000 mesh, and then put in a suitable container, an appropriate amount of water is added to absorb pearl water, and then extra water is added. At this time, wash filtration is performed 1-2 times.

The solution is stirred at 350 to 600 rpm while slowly adding 5-20% of an edible glacial acetic acid aqueous solution to the washed pearl solid. At this time, the reaction temperature is maintained at room temperature. When the reaction does not occur when carbon dioxide is generated, the supernatant (first liquid) is recovered. The first liquid is a clear-grey-white clear aqueous solution containing calcium acetate. The amount of the first liquid is determined according to the acid and concentration of the pearl to be added.

At this time, the main component of glacial acetic acid is acetic acid (CH ₃ COOH), the main component of pearl is calcium carbonate (CaCO ₃ ), so their reaction in the aqueous solution is as follows.

CaCO ₃ + 2CH ₃ COOH Ca (CH ₃ COO) ₂ + H ₂ O + CO ₂ ↑

After all, the main component of the water-soluble pearl calcium prepared by reacting pearl powder and glacial acetic acid is a large amount of calcium acetate and hydrolyzed trace protein.

After completion of the first stage reaction, the supernatant is centrifuged or filtered off, and the precipitate is washed several times with purified or distilled water so that the pH of the final washing solution is neutral.

2 to 10 times the amount of purified water was added to the washed solid of the first step, and the mixture was stirred at 350 to 600 rpm for 1 hour at room temperature to elute the water-soluble residual component and then separated by filtration (second solution).

The first aqueous solution and the second liquid were mixed and then neutralized with direct or dilute alkaline solution to prepare an aqueous pearl solution with calcium concentration of 20,000 ppm or more and protein concentration of 0.1-1.0 mg / ml (third solution). .

The third liquid may be prepared by using all drying methods, but preferably spray dried at a temperature of 100 or more to prepare a water-soluble pearl powder. This powder may be used as it is, but in order to improve functionality, a water-soluble pearl powder is prepared by mixing a mushroom polysaccharide extract, mucopolysaccharide protein, silk amino acid, vitamin D and the like.

The concentration of calcium in pearls was measured by atomic absorption spectrometry (ASS) or inorganic ion measurement, and the concentration of protein was measured using semi-Kjeldahl method.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Examples, but the scope of the present invention is not limited to the following Examples.

Example 1 Physical Method (Pretreatment Preparation Example 1)

50g each of cultured sea pearls and freshwater pearls are placed in two containers, 2 ~ 3 times of purified water or distilled water is added, mixed well, then left to remove the supernatant, and centrifuged 2 ~ 3 times in the same manner. Or by filtration washing to separate the solids.

Example 2. Chemical Method (Acid Hydrolysis Preparation Example 2)

About 400 ml of 7% edible glacial acetic acid was slowly added to 100 g of the solid obtained in Preparation Example 1.

Mixing and stirring were used, and the reaction temperature was room temperature. When bubbles no longer occur and a yellow liquid is produced, it is cooled to room temperature and divided into pearl calcium-acetate (liquid) and the remaining insoluble matter (solid content). After reacting for about 2-3 hours, 1300 ml of a clear aqueous solution was separated by filtration (second solution). The second solution contains 20,000 ppm of calcium and a small amount of protein. Repeat this process until the pH of the final wash water is neutralized by washing it 2-3 times with about 2-3 times the amount of purified water or distilled water.

Example 3, dry powder preparation (water soluble calcium and protein-containing powder)

Water-soluble pearl calcium and protein powder were prepared by spray drying the solutions obtained in Preparation Examples 2 and 3. Water-soluble pearl calcium powder prepared by the above method was obtained 0.8 ~ 0.95 by weight relative to the raw material.

Example 4 Preparation of Powder Containing Water Soluble Pearl Calcium and Functional Ingredient

To prepare a functional water-soluble pearl powder by mixing the powder obtained in Example 1, mushroom polysaccharide extract, mucopolysaccharide protein, silk amino acid, vitamin D and the like.

Therefore, the present invention can increase the content of calcium and active protein by preparing a water-soluble pearl powder using physical and chemical methods.

In addition, mushroom polysaccharide extract, mucopolysaccharide protein, silk amino acid, vitamin D, etc. are mixed to increase the body's calcium absorption rate, it has the effect of preventing immune strengthening and skin aging.

Claims (5)

The pearls were pulverized to a particle size of 500 to 1,000 mesh, and then edible glacial acetic acid aqueous solution was added to the solid obtained by filtration and washing at 2 to 3 times the water at room temperature, and then reacted at 350 to 600 rpm until no carbon dioxide was generated. A first step of precipitating solids and separating the first liquid; A second step of separating purified water by adding purified water to the solid content obtained in the first step and reacting the reaction; A third step of preparing a water-soluble pearl calcium and a protein powder by mixing the first liquid and the second liquid by spray drying; Method for producing a water-soluble pearl powder using the acid hydrolysis method at room temperature, characterized in that the powder obtained in the third step, the mushroom polysaccharide extract, mucopolysaccharide protein, silk amino acid, vitamin D and the like is prepared by mixing. The method of claim 1, Pearl is a natural freshwater culture, or a sea-cultured pearl, the method of producing a water-line pearl powder using the acid hydrolysis method at room temperature, characterized in that it is produced using physical and chemical methods. The method of claim 1, The method of producing a water-soluble pearl powder using an acid hydrolysis method at room temperature, characterized in that the concentration of the edible glacial acetic acid solution is 1 to 20% (v / v). The method of claim 1, The method for producing a water-soluble pearl powder using an acid hydrolysis method at room temperature, characterized in that the reaction temperature of the second step is room temperature. The method of claim 1, In addition to the edible glacial acetic acid aqueous solution, water-soluble using the acidic hydrolysis method at room temperature, characterized by reacting an organic acid such as lactic acid, citric acid, kojic acid, glycolic acid having a concentration of 1 to 20% (v / v) alone or by mixing with glacial acetic acid. Method for preparing pearl powder.