CN110915827A - Targeted deinsectization and deodorization biological agent and preparation method thereof - Google Patents
Targeted deinsectization and deodorization biological agent and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
Abstract
The invention belongs to the technical field of biological agents and preparation processes thereof, and discloses a targeting deinsectization and deodorization biological agent and a preparation method thereof. The preparation is obtained by combining medicinal materials with sterilizing effect with expression polypeptide with deinsectization and sterilization effect obtained by protein engineering expression. The raw materials are mutually fused and interacted, so that the aim of deinsectization is effectively fulfilled, and meanwhile, the living microbial thallus survival quantity is supplemented by adding the viable bacteria agent, so that the aim of deodorization is fulfilled. The preparation directly promotes the removal of polypide by using a targeted gene expression mode, and also has no influence on other living things, thereby achieving high removal rate, maintaining the balance of ecological environment and having no pollution. The preparation process is simple and convenient in operation process, few in operation equipment, low in cost, safe and reliable under the condition of ensuring good mixing of the raw materials.
Description
Technical Field
The invention belongs to the technical field of biological agents and preparation processes thereof, and particularly relates to a targeted deinsectization and deodorization biological agent and a preparation method thereof.
Background
Crops, vegetables and fruits are often affected by pests and diseases during the growth process, so that the quality of the crops, the vegetables and the fruits is easily reduced or damaged, and even the crops, the vegetables and the fruits cannot grow continuously. At present, in order to prevent and treat the influence of plant diseases and insect pests of crops, people generally use pesticides to treat the diseases and insect pests, but currently used pesticide products all cause pesticide residues on the crops, vegetables and fruits, and then harm is caused to users and the health and safety of eaters are seriously affected.
Meanwhile, the currently commonly used pesticide disinsection method can directly cause a great amount of death of most microorganisms and microbial enzymes in farmland soil where crops and vegetables and fruits are located, so that soil hardening is promoted, roots of the crops and the vegetables and fruits are rotten and smelly, and the yield of the crops and the vegetables and fruits is greatly influenced. Therefore, it is an urgent problem to solve the above problems, which is to provide a pesticide having an effect of killing insects and not causing other offensive odors.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a targeted deinsectization and deodorization biological agent and a preparation method thereof.
The technical scheme adopted by the invention is as follows: a targeting deinsectization and deodorization biological preparation is mainly prepared from the following raw materials in parts by weight:
20-220 parts of traditional Chinese medicine extract, 10-90 parts of accelerant, 20-80 parts of nano titanium dioxide, 50-180 parts of gene deinsectization peptide, 50-180 parts of gene antibacterial peptide, 80-270 parts of viable bacteria agent and 350 parts of solvent.
The gene antibacterial peptide selected in the targeted deinsectization and deodorization biological agent is a polypeptide which is obtained by expression after the DNA of a certain strain is specifically killed through gene recombination, and the polypeptide has directivity. The range of the strains protected by the gene antibacterial peptide in the compatibility mode is not overlapped with the range included by the viable bacteria agent, and the selection of the strains is independent.
The targeted deinsectization and weeding biological preparation is obtained by combining medicinal materials with the effects of killing viruses and sterilizing and expression polypeptides with the effects of killing insects and sterilizing, which are obtained by protein engineering expression. The raw materials in the targeted deinsectization and weeding biological agent are fused and interacted with each other, so that the aim of deinsectization is effectively fulfilled, and meanwhile, the living microbial thallus survival quantity is supplemented by adding the viable bacteria agent, so that the aim of deodorization is fulfilled. The preparation directly promotes the removal of polypide by using a targeted gene expression mode, and also has no influence on other living things, thereby achieving high removal rate, maintaining the balance of ecological environment and having no pollution.
Preferably, the preparation is mainly prepared from the following raw materials in parts by mass:
80-170 parts of traditional Chinese medicine extract, 30-70 parts of accelerant, 40-68 parts of nano titanium dioxide, 70-150 parts of gene deinsectization peptide, 70-150 parts of gene antibacterial peptide, 230 parts of viable bacteria agent and 300 parts of solvent 150.
Preferably, the preparation is mainly prepared from the following raw materials in parts by mass:
150 parts of traditional Chinese medicine extract, 55 parts of accelerant, 53 parts of nano titanium dioxide, 135 parts of gene deinsectization peptide, 135 parts of gene antibacterial peptide, 180 parts of viable bacteria agent and 245 parts of solvent.
The nanometer titanium dioxide is white loose powder, has strong ultraviolet shielding effect and good dispersibility and weather resistance. Can be sterilized for a long time under the action of ultraviolet rays in light. The bacillus subtilis can be used for thoroughly killing malignant hela cells, and the killing rate of spores, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, salmonella, dental branch bacteria and aspergillus of black varieties of bacillus subtilis reaches more than 98%. It can also be used for the production of antibacterial pollution products with the functions of sterilization, antifouling, deodorization and self-cleaning.
Preferably, the Chinese medicinal extract comprises one or more of fructus Zanthoxyli extract, radix Sophorae Flavescentis extract, fructus Toosendan extract, Berberis pruinosa extract and Galla chinensis extract;
preferably, the mass ratio of the pepper extract, the sophora flavescens extract, the chinaberry extract, the berberis pruinosa extract and the gallnut extract is 4-6:2-5:0.5-1: 3-8: 0.5-1.
Zanthoxylum bungeanum Maxim is Rutaceae, Zanthoxylum genus deciduous tree. The wood of pricklyash peel is typically light yellow, the color of pricklyash peel is changed into dark yellow when exposed in air, the core material and the border material are not obviously distinguished, the xylem structure is dense and uniform, the longitudinal section of pricklyash peel has silk luster, the peel can be used as a seasoning, aromatic oil can be extracted, the pricklyash peel can be used as a medicine, seeds can be eaten, and soap can also be processed and prepared. The pricklyash peel has the efficacy of dehumidifying and killing insects,
radix Sophorae Flavescentis (Latin name: Sophoraflavoscens Ait.), is the dried root of Sophora flavescens Ait of Leguminosae, Sophora, and is called Diphora japonica, Haohanshi, Sophora japonica or Sophora japonica. Has effects of clearing heat, eliminating dampness, and killing parasite. The radix Sophorae Flavescentis extract has inhibitory effect on Bacillus tuberculosis, Bacillus dysenteriae, Staphylococcus aureus, and Escherichia coli. The root of radix Sophorae Flavescentis contains matrine and laburnine, and has effects of clearing heat and promoting diuresis, resisting bacteria and diminishing inflammation, invigorating stomach and expelling parasites, the seed can be used as pesticide, and the stem bark fiber can be used as woven sack.
Melia azedarach, also known as Chinaberry, a well-known variety of Meliaceae plants, also known as Kuling, Chinaberry, and forest trees. Melia azedarach is a deciduous tree of Meliaceae, and has dark brown bark, longitudinal fissure, purple old branch, and many tiny skin pores. The plants are medicinal plants, and flowers, leaves, fruits and root barks of the plants can be used as medicines, and the root barks can expel ascaris and hookworm, but are toxic and strict in medical advice when used. The cortex Meliae has insecticidal effect, the folium Meliae has insecticidal effect, and the fruit and flower of Melia azedarach have insecticidal effect.
Berberis pruinosa (Berberis thunbergii bar. Atropurpurea Chenault), also known as Berberis pruinosa, is Berberis prunifolia, belonging to the order Ranunculaceae, the genus Berberis. It is native to the south-east China, North China and Qinling mountain. The root part and stem part of Berberis pruinosa L.contain berberine, which can be used as raw material for extracting berberine.
Galla chinensis (Latin's name: Rhus chinensis Mill.), Baichongcang and Baiyao decoction are prepared by parasitizing Aphis angustifolia or Aphis ovalicaulis female of the same family Aphididae on tender leaves or leafstalks of Rhus chinensis and other plants of the same genus of the Rhus chinensis, stabbing to generate a sac-shaped poly-organism gall, and baking and drying. The Galla chinensis extract has inhibitory effect on Staphylococcus aureus, Shigella flexneri, cholera suis and Escherichia coli.
Preferably, the promoter comprises bacillus, preferably bacillus comprises one or more of bacillus subtilis, bacillus thuringiensis, bacillus laterosporus, and bacillus sphaericus.
Preferably, the viable bacteria agent comprises EM bacteria, preferably, the EM bacteria comprise one or more of photosynthetic bacteria, lactic acid bacteria, yeast and actinomycetes.
Preferably, the solvent comprises water and/or absolute ethanol.
Preferably, the deinsectization peptide is obtained by obtaining a DNA sequence of a target gene from a gene library, amplifying the DNA sequence in vitro by a PCR (polymerase chain reaction) technology, recombining the target gene into a phytotoxin DNA sequence by a gene recombination technology, and expressing the DNA sequence.
Preferably, the gene antibacterial peptide is obtained by amplifying a DNA sequence of a target gene obtained from a gene library in vitro by a PCR (polymerase chain reaction) technology, recombining the target gene into the DNA sequence of the insect toxin by using a gene recombination technology, and expressing.
A preparation method of a biological preparation for targeted deinsectization and deodorization comprises selecting traditional Chinese medicine extract, promoter, nanometer titanium dioxide, gene deinsectization peptide, gene antibacterial peptide, viable bacteria agent and solvent according to corresponding proportion, and mixing uniformly to obtain the final product.
The preparation process of the targeted deinsectization and deodorization biological agent has the advantages of simple operation process, less operation equipment, low cost, safety and reliability under the condition of ensuring good mixing of raw materials.
The invention has the beneficial effects that:
the invention provides a targeted deinsectization and deodorization biological agent, which is obtained by combining medicinal materials with the effects of killing virus and sterilization with expression polypeptides with the effects of deinsectization and sterilization, which are obtained by protein engineering expression. The raw materials in the targeted deinsectization and weeding biological agent are fused and interacted with each other, so that the aim of deinsectization is effectively fulfilled, and meanwhile, the living microbial thallus survival quantity is supplemented by adding the viable bacteria agent, so that the aim of deodorization is fulfilled. The preparation directly promotes the removal of polypide by using a targeted gene expression mode, and also has no influence on other living things, thereby achieving high removal rate, maintaining the balance of ecological environment and having no pollution. The preparation process of the targeted deinsectization and deodorization biological agent has the advantages of simple operation process, less operation equipment, low cost, safety and reliability under the condition of ensuring good mixing of raw materials.
Detailed Description
The present invention is further illustrated below with reference to specific examples. It will be appreciated by those skilled in the art that the following examples, which are set forth to illustrate the present invention, are intended to be part of the present invention, but not to be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples were carried out under the conventional conditions, unless otherwise specified. The reagents used are all conventional products which are commercially available.
Example 1:
selecting 20 g of pepper extract, 10 g of bacillus subtilis solution, 20 g of nano titanium dioxide, 50 g of gene deinsectization peptide, 50 g of gene antibacterial peptide, 80 g of EM bacteria and 100 g of water according to corresponding proportion, and uniformly mixing to obtain a finished product.
Example 2:
220 g of pepper extract, 90 g of bacillus subtilis solution, 80 g of nano titanium dioxide, 180 g of gene deinsectization peptide, 180 g of gene antibacterial peptide, 270 g of EM bacteria and 350 g of water are selected according to corresponding proportion and mixed evenly to obtain a finished product.
Example 3:
selecting 20 g of sophora flavescens extract, 10 g of bacillus thuringiensis solution, 20 g of nano titanium dioxide, 50 g of gene deinsectization peptide, 50 g of gene antibacterial peptide, 80 g of photosynthetic bacteria and 100 g of absolute ethyl alcohol according to corresponding proportion, and uniformly mixing to obtain a finished product.
Example 4:
selecting 220 g of sophora flavescens extract, 90 g of bacillus thuringiensis solution, 80 g of nano titanium dioxide, 180 g of gene deinsectization peptide, 180 g of gene antibacterial peptide, 270 g of photosynthetic bacteria and 350 g of absolute ethyl alcohol according to corresponding proportion, and uniformly mixing to obtain a finished product.
Example 5:
selecting 80 g of chinaberry extract, 30 g of bacillus subtilis solution, 40 g of nano titanium dioxide, 70 g of gene deinsectization peptide, 70 g of gene antibacterial peptide, 120 g of photosynthetic bacteria and 150 g of water according to corresponding proportion, and mixing uniformly to obtain a finished product.
Example 6:
170 g of chinaberry extract, 70 g of bacillus subtilis solution, 68 g of nano titanium dioxide, 150 g of gene deinsectization peptide, 150 g of gene antibacterial peptide, 230 g of photosynthetic bacteria and 300 g of water are selected according to corresponding proportion and mixed evenly to obtain a finished product.
Example 7:
80 g of berberis pruinosa extract, 30 g of bacillus thuringiensis solution, 40 g of nano titanium dioxide, 70 g of gene deinsectization peptide, 70 g of gene antibacterial peptide, 120 g of photosynthetic bacteria and 150 g of absolute ethyl alcohol are selected according to corresponding proportion and mixed evenly to obtain a finished product.
Example 8:
170 g of berberis pruinosa extract, 70 g of bacillus thuringiensis solution, 68 g of nano titanium dioxide, 150 g of gene deinsectization peptide, 150 g of gene antibacterial peptide, 230 g of photosynthetic bacteria and 300 g of absolute ethyl alcohol are selected according to corresponding proportion and mixed evenly to obtain a finished product.
Example 9:
according to the corresponding proportion, 80 g of Chinese gall extract, 30 g of bacillus thuringiensis solution, 40 g of nano titanium dioxide, 70 g of gene deinsectization peptide, 70 g of gene antibacterial peptide, 120 g of photosynthetic bacteria and 150 g of absolute ethyl alcohol are selected and mixed evenly to obtain the finished product.
Example 10:
170 g of Chinese gall extract, 70 g of bacillus thuringiensis solution, 68 g of nano titanium dioxide, 150 g of gene deinsectization peptide, 150 g of gene antibacterial peptide, 230 g of photosynthetic bacteria and 300 g of absolute ethyl alcohol are selected according to corresponding proportion and mixed evenly to obtain a finished product.
Example 11:
according to the corresponding proportion, 60 g of pepper extract, 30 g of sophora flavescens extract, 7.5 g of chinaberry extract, 45 g of berberis pruinosa extract, 7.5 g of gallnut extract, 55 g of bacillus subtilis solution, 53 g of nano titanium dioxide, 135 g of gene avermectin, 135 g of gene antibacterial peptide, 180 g of actinomycete and 245 g of water are selected and mixed evenly to obtain the finished product.
Example 12:
according to the corresponding proportion, 60 g of pepper extract, 30 g of sophora flavescens extract, 7.5 g of chinaberry extract, 45 g of berberis pruinosa extract, 7.5 g of Chinese gall extract, 55 g of bacillus thuringiensis solution, 53 g of nano titanium dioxide, 135 g of gene avermectin, 135 g of gene antibacterial peptide, 180 g of microzyme and 245 g of absolute ethyl alcohol are selected and mixed evenly to obtain the finished product.
Example 13:
43 g of pepper extract, 36 g of sophora flavescens extract, 7 g of chinaberry extract, 57 g of berberis pruinosa extract, 7 g of gallnut extract, 55 g of bacillus subtilis solution, 53 g of nano titanium dioxide, 135 g of gene avermectin, 135 g of gene antibacterial peptide, 180 g of photosynthetic bacteria and 245 g of water are selected according to corresponding proportion and mixed evenly to obtain a finished product.
Example 14:
according to the corresponding proportion, 60 g of pepper extract, 30 g of sophora flavescens extract, 7.5 g of chinaberry extract, 45 g of berberis pruinosa extract, 7.5 g of Chinese gall extract, 55 g of bacillus thuringiensis solution, 53 g of nano titanium dioxide, 135 g of gene avermectin, 135 g of gene antibacterial peptide, 180 g of photosynthetic bacteria and 245 g of absolute ethyl alcohol are selected and mixed evenly to obtain the finished product.
In the products prepared from the targeted deinsectization and deodorization biological agent, the gene antibacterial peptide and the gene deinsectization peptide are in colorless transparent liquid states. The expression and characteristics of the protein polypeptide are that the protein polypeptide is often used as a virus gene regulation inhibitor, and the gene peptide interferes with virus gene regulation to inhibit virus replication, thereby achieving the effect of killing viruses. The natural virus gene regulation inhibitor is difficult to prepare, has low yield, high cost and insufficient purity, and limits clinical application. The gene peptide is a gene engineering product expressed by gene recombination, and is a high-purity water-soluble protein produced by a DNA recombination technology. It has broad-spectrum antiviral and immunoregulating bioactivity. Meanwhile, the compound is used as a protein product, has the characteristics of safety, difficult generation of drug resistance and incapability of common antibiotics, has definite curative effect, and is an excellent product for preventing and treating viral diseases of livestock and poultry.
Examples of the experiments
Experimental reagent: the preparation method of each embodiment is selected, the three-chemical borer DNA is selected for recombination when the gene avermectin is subjected to gene recombination combination, then the gene three-chemical borer removing peptide is obtained through expression, and then the finished products of each embodiment are obtained according to the preparation method. The imidacloprid monosultap (brand: nong dao sheng, dosage form: wettable powder, manufacturer: Luoyang Gui duo Biotech Co., Ltd.) is selected.
Subject: selecting a paddy field with the three-chemical borer pest, extracting 1500mL of water sample from the paddy field, uniformly dividing the water sample into 15 parts, each part being 100mL, and numbering the water sample to be 1-11. Wherein, the codes of 1-10 are experimental groups, and the embodiment finished products corresponding to the codes in the above embodiments are used. Code 11 is a control group, and pyraclostrobin is used.
And (3) experimental detection: and recording the number of the three-chemical borers in each detected water sample, and then recording the survival number of the three-chemical borers after adding the experimental reagent for 12 h.
The experimental indexes are as follows: the insecticidal rate is (total number of the experimental starting three-chemical borers-12 h survival number of the three-chemical borers)/total number of the experimental starting three-chemical borers
The results are shown in the following table:
TABLE 1 SANHUA borer eliminating effect experiment result data table
The data result shows that the products prepared by the various examples of the targeted deinsectization and deodorization biological agent have remarkable effects from the examples 1-14 after the spraying is finished, the removal rate reaches 50% or more, and the removal rate of the control group is 56%, which is almost the same as the removal effect of the example 1.
The putrefactive odor of the water samples is obviously weakened to disappear after the water samples are subjected to 12 hours detection.
The invention provides a targeted deinsectization and deodorization biological agent, which is obtained by combining medicinal materials with the effects of killing virus and sterilization with expression polypeptides with the effects of deinsectization and sterilization, which are obtained by protein engineering expression. The raw materials in the targeted deinsectization and weeding biological agent are fused and interacted with each other, so that the aim of deinsectization is effectively fulfilled, and meanwhile, the living microbial thallus survival quantity is supplemented by adding the viable bacteria agent, so that the aim of deodorization is fulfilled. The preparation directly promotes the removal of polypide by using a targeted gene expression mode, and also has no influence on other living things, thereby achieving high removal rate, maintaining the balance of ecological environment and having no pollution. The preparation process of the targeted deinsectization and deodorization biological agent has the advantages of simple operation process, less operation equipment, low cost, safety and reliability under the condition of ensuring good mixing of raw materials.
While particular embodiments of the present invention have been illustrated and described, it will be appreciated that the present invention is not limited to the above-described alternative embodiments, and that various other forms of product may be devised by anyone in light of the present invention. The foregoing detailed description should not be construed as limiting the scope of the invention, and those skilled in the art will understand that various modifications can be made to the technical solutions described in the foregoing embodiments, or some or all of the technical features can be equivalently replaced, without departing from the spirit and scope of the invention, and at the same time, such modifications or replacements do not cause the essence of the corresponding technical solutions to depart from the scope of the technical solutions of the embodiments of the invention; the scope of the invention should be determined with reference to the appended claims, and the description should be construed to interpret the claims.
Claims (10)
1. The targeted deinsectization and deodorization biological agent is characterized by being prepared from the following raw materials in parts by mass:
20-220 parts of traditional Chinese medicine extract, 10-90 parts of accelerant, 20-80 parts of nano titanium dioxide, 50-180 parts of gene deinsectization peptide, 50-180 parts of gene antibacterial peptide, 80-270 parts of viable bacteria agent and 350 parts of solvent.
2. The biological preparation for targeting deinsectization and deodorization of claim 1, wherein the preparation is prepared from the following raw materials in parts by mass:
80-170 parts of traditional Chinese medicine extract, 30-70 parts of accelerant, 40-68 parts of nano titanium dioxide, 70-150 parts of gene deinsectization peptide, 70-150 parts of gene antibacterial peptide, 230 parts of viable bacteria agent and 300 parts of solvent 150.
3. The biological preparation for targeting deinsectization and deodorization of claim 2, wherein the preparation is prepared from the following raw materials in parts by mass:
150 parts of traditional Chinese medicine extract, 55 parts of accelerant, 53 parts of nano titanium dioxide, 135 parts of gene deinsectization peptide, 135 parts of gene antibacterial peptide, 180 parts of viable bacteria agent and 245 parts of solvent.
4. The biological preparation according to any one of claims 1 to 3, wherein the herbal extract comprises one or more of Zanthoxylum bungeanum extract, Sophora flavescens extract, Melia azedarach extract, Berberis pruinosa extract and Galla rhois extract;
preferably, the mass ratio of the pepper extract, the sophora flavescens extract, the chinaberry extract, the berberis pruinosa extract and the gallnut extract is 4-6:2-5:0.5-1: 3-8: 0.5-1.
5. The targeted disinsection and deodorization biological agent as recited in any one of claims 1-3, wherein said promoter comprises Bacillus, preferably said Bacillus comprises one or more of Bacillus subtilis, Bacillus thuringiensis, Bacillus laterosporus, and Bacillus sphaericus.
6. A targeted insect control and deodorization biological agent as claimed in any one of claims 1-3, wherein said viable bacteria agent comprises EM bacteria, preferably said EM bacteria comprises one or more of photosynthetic bacteria, lactic acid bacteria, yeast and actinomycetes.
7. The targeted insecticidal and deodorant biological agent according to any one of claims 1-3, wherein the solvent comprises water and/or anhydrous ethanol.
8. The biological agent for targeted disinsection and deodorization according to any one of claims 1-3, wherein the gene deinsectization peptide is obtained by obtaining DNA sequence of target gene from gene library, amplifying in vitro by PCR polymerase chain reaction technique, recombining target gene into phytotoxin DNA sequence by gene recombination technique, and expressing.
9. The biological preparation according to any one of claims 1 to 3, wherein the gene antibacterial peptide is obtained by amplifying a DNA sequence of a target gene obtained from a gene library in vitro by PCR polymerase chain reaction, recombining the target gene into an insect toxin DNA sequence by gene recombination, and expressing the recombinant gene.
10. The preparation method of a biological agent for targeted deinsectization and deodorization according to any one of claims 1-9, wherein the preparation method comprises selecting traditional Chinese medicine extract, promoter, nano titanium dioxide, gene deinsectization peptide, gene antibacterial peptide, viable bacteria agent and solvent according to corresponding proportion, and mixing uniformly to obtain the final product.
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