CN110904089B - Diversified microorganism system for improving eutrophic water body and application thereof - Google Patents
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Abstract
The invention belongs to the technical field of environmental management, and particularly relates to a diversified microorganism system for improving eutrophic water and application thereof. The diversified microorganism system provided by the invention can enhance the adaptability of microorganism products in different environments, thereby improving the stability of eutrophic water degradation and aiming at overcoming the problems of weak adaptability and unstable effect of the existing microorganism water purification technology in different water qualities. The microbial system comprises a diversified microbial inoculum and an additive, wherein the diversified microbial inoculum comprises bacillus subtilis liquid; a bacillus megaterium solution; b, a Brevibacillus laterosporus bacterial liquid; a lactic acid bacteria liquid; nitromonas bacterium liquid; a bacillus licheniformis liquid; green wood fungus liquid; a saccharomyces cerevisiae bacterial liquid; pseudomonas fluorescens liquid; rhodospirillum rubrum liquid; rhodopseudomonas palustris bacterial liquid; spherical rhodobacter bacteria liquid; pseudomonas putida bacterial liquid; the additive comprises starch, diatomite, calcium carbonate and compound amino acid.
Description
Technical Field
The invention belongs to the technical field of environmental management, and particularly relates to a diversified microorganism system for improving eutrophic water and application thereof.
Background
Healthy water has a variety of metrics and parameters. Among them, the content of organic nutrients such as nitrogen and phosphorus is an important index for sustainable ecology. However, the intensive development and utilization of environmental resources in recent years have led to the discharge of a large amount of pollutants rich in nutrients into various water bodies such as rivers, lakes and the like. When the intake of nutrient substances exceeds the speed of natural circulation metabolism in the water body, the water body presents eutrophication pollution. The eutrophic polluted water body has poor smell and appearance, the water body image is seriously influenced, the ecological system in the water body is damaged due to the unbalanced intake and consumption of nutrient substances, and the formed biotoxin, the proliferation of harmful species and the excessive propagation of algae can also cause damage to phytoplankton, fishes and shrimps and finally ingested people and livestock in the water body.
At present, the eutrophic water body is mainly treated by physical and chemical means. The water body can be effectively improved by methods of changing water, excavating sediment, increasing oxygen, aerating and the like. However, the treatment mode is often limited to a small area of water body, and has the disadvantages of non-continuous effective effect, high cost and the like. Although chemical means such as coagulation sedimentation and chemicals against harmful algae can reduce suspended organic pollutants in a short time, they have new ecological problems such as secondary pollution caused by incomplete treatment, high cost, chemical residue and the like. In view of the above, the biological method for remedying the eutrophication problem caused by organic substances has been developed rapidly in recent years, and particularly, the method for effectively converting and degrading excessive nitrogen and phosphorus in water by using the principle that microorganisms take up organic substances as nutrients.
Compared with the traditional physical and chemical means, the microbial remediation technology has the advantages of low cost, thorough degradation, short remediation time, simple control and manufacture and the like. However, the microbial remediation system has many problems, and one of the serious problems is that the degradation effect of the microbes is affected by the water quality and is unstable. The microorganisms are various, and different species have differences in nutrient intake, metabolic capability and metabolic byproducts. The degradation effect of the microorganisms directly depends on the adaptation and reproductive capacity of the microorganisms in the polluted water body. The pollution degree and pollutant components of different water bodies are different, and the existing microbial technology is lack of biological diversity and has fewer or even single types, so that the adaptability of the microbes to the polluted water bodies is unstable, and the degradation effect is seriously influenced.
Disclosure of Invention
The invention provides a diversified microorganism system for improving eutrophic water and application thereof, aiming at solving part of problems in the prior art or at least relieving part of problems in the prior art.
The invention is realized in such a way that a diversified microorganism system for improving eutrophic water comprises a diversified microbial inoculum and an additive,
the diversified microbial inoculum comprises the following components in parts by mass: 5-10 parts of bacillus subtilis liquid; 5-10 parts of bacillus megaterium liquid; 3-5 parts of a brevibacillus laterosporus bacterial liquid; 1-3 parts of lactobacillus liquid; 3-5 parts of nitromonas bacterium liquid; 5-10 parts of bacillus licheniformis liquid; 1-3 parts of a green wood fungus liquid; 1-3 parts of saccharomyces cerevisiae bacterial liquid; 1-3 parts of pseudomonas fluorescens bacterial liquid; 1-3 parts of rhodospirillum rubrum liquid; 1-3 parts of rhodopseudomonas palustris bacterial liquid; 3-5 parts of spherical rhodobacter bacteria liquid; 3-5 parts of pseudomonas putida bacterial liquid;
the additive comprises starch, diatomite, calcium carbonate and compound amino acid, wherein the mass ratio of the starch to the diatomite is (1-2): (3-5): (3-5): (0.2-0.5);
the dosage proportion of the diversified microbial inoculum and the additive is (1-2L): (50-100 kg).
Further, the order of magnitude of the bacterial cells in each bacterial liquid of the diversified bacterial agents is 106-108CFU/mL。
Further, a culture medium for culturing each strain in the diversified microbial inoculum comprises the following components in percentage by mass: 5-10% of soybean peptone; 2.5 to 5 percent of glucose; 0.5 to 1 percent of sodium chloride; 0.5 to 1 percent of calcium chloride; 0.5 to 1 percent of magnesium sulfate; 0.5 to 1 percent of potassium hydrogen phosphate.
Further, the mixing method of the diversified microbial inoculum and the additive comprises the following steps: mixing the prepared mixed microbial inoculum and additives in proportion to obtain a carrier attached with the compound microorganisms, and then airing, stirring and crushing the carrier into powder.
The application of the diversified microorganism system for improving the eutrophic water body in the water body treatment is disclosed.
In summary, the advantages and positive effects of the invention are:
the invention aims to solve the problems of weak adaptability and unstable effect of the existing microbial water purification technology in different water qualities, and provides a diversified microbial system for improving eutrophic water, which enhances the adaptability of microbial products in different environments, thereby improving the degradation stability of the eutrophic water.
There are various challenges in preparing a diverse microbial system that meets the requirements. Firstly, diversified competition or synergistic relationship exists among various microorganisms, and the selection of compatible microorganism species in the polluted water body is very important. Secondly, the pollutants in the eutrophic water are various. The degradation mechanisms of pollutants such as ammonia, nitrogen, phosphorus, etc. are different. The diverse microbial systems require the existence of microbial species that can implement various degradation mechanisms. If the problems can be solved, the microorganism repairing system is a choice with multiple advantages, can effectively replace the traditional physical chemistry and other modes, and effectively solves the eutrophication problem of large-area water bodies.
The invention scientifically and reasonably integrates beneficial floras of bacillus subtilis, bacillus megaterium, bacillus brevis, lactic acid bacteria, nitromonas, bacillus licheniformis, trichoderma viride, saccharomyces cerevisiae, pseudomonas fluorescens, rhodospirillum rubrum, rhodopseudomonas palustris, rhodobacter sphaeroides and pseudomonas putida, and the composite microbial agent is prepared by an advanced production process and consists of a composite microbial flora, a carrier and nutrient substances. The functional microorganisms in the microbial inoculum have the characteristics of variety diversity and degradation of different pollutants, and the microorganisms interact with each other to form a symbiotic system with complex composition and stable structure. After the fertilizer is applied to a water body, microorganisms degrade pollutants by secreting various extracellular enzymes or intracellular enzymes, decompose organic matters in the eutrophic water body into simple inorganic matters and synergistically decompose harmful substances such as ammonia nitrogen, nitrous acid, hydrogen sulfide and the like. Photosynthetic bacteria in the microbial inoculum carry out photosynthesis by utilizing light energy and simple inorganic matters in water, and generate oxygen while fixing elements such as nitrogen, phosphorus and the like in water, so that the oxygen content in water is increased, and the transparency of the water is improved; and stabilizing the pH value of the water body. The carrier in the microbial system can provide an attachment place for the microbes, is convenient for product release, and provides a certain degree of protection for the microbes in a strange environment. Meanwhile, the carrier contains a small amount of nutrient components, so that nutrients are provided for the initial propagation of the microorganisms, and the adaptability of the microorganisms to the environment is enhanced.
Compared with the existing mixed microorganism system, the microorganism integrated by the invention is effectively directed to various environments and pollution sources in the water body in terms of mechanism. The treatment of the eutrophic water body by the bacillus subtilis, the bacillus megaterium, the bacillus laterosporus and the bacillus licheniformis is proved, and the method has the advantage of strong vitality adaptability, but the action modes of the bacillus subtilis, the bacillus megaterium, the bacillus laterosporus and the bacillus licheniformis are different. The denitrification effect of the bacillus subtilis is particularly excellent; the bacillus megaterium can more effectively aim at phosphorus in the water body; the brevibacillus laterosporus can effectively degrade the tenacious dye substances causing water body dyeing; the bacillus licheniformis has effective inhibition effect on excessive pathogenic bacteria in the polluted water body. The lactobacillus can effectively reduce the biological and chemical oxygen consumption in the sewage treatment process. The nitrifying monad converts excessive ammonia in the eutrophic water body into nitrate to realize denitrification reaction. The green wood fungi can effectively polymerize organic pollution particles into easily salvageable organic mycelia in a water body with serious suspended organic pollution particles. The saccharomyces cerevisiae can effectively reduce the phosphorus content in the water body in the eutrophic water body with the overproof phosphorus content. The pseudomonas fluorescens and the rhodopseudomonas palustris can effectively degrade cyanide and iodide and play a role in decoloring severely dyed eutrophic water. The rhodospirillum rubrum also has a denitrification effect in eutrophic water bodies and has strong adaptability to water bodies in different illumination environments. The rhodobacter sphaeroides has excellent degradation effect on eutrophic water with high protein content. The pseudomonas putida can effectively target phenolic compounds in the eutrophic water body. From the preparation point of view, the separated culture mode adopted by the invention can ensure that the strains reach high density and high activity before mixing, and avoid the phenomenon of strain community imbalance caused by mutual nutrient competition among strains possibly generated in the preparation process so as to maintain the basic content of various microorganisms in the final product.
The effects of two eutrophic experimental water bodies with different pollution conditions are obvious, and experimental data show that the diversified composite microbial system can still effectively treat eutrophic organic pollution under the condition that pollution degrees, geographical positions and pollution projects are different; can solve the problems of weak adaptability and unstable effect of the existing microorganism water purification technology in different water qualities. The first application example of the invention is a water body with serious ammonia nitrogen exceeding standard. After the application, the total ammonia, the total nitrogen and the comprehensive ammonia nitrogen are respectively reduced by 71 percent, 67 percent and 92 percent. Compared with the prior cases, the microbial system of photosynthetic bacteria, actinomycetes, saccharomycetes and lactic acid bacteria is adopted for treating the similar water body, and the long-term treatment effect on the total ammonia and the total nitrogen only reaches about 20-40% (see the literature: the effect of controlling blue-green algae in eutrophic lakes by effective microbiota 2000; the test of restoring river water in west dams by liquid microbial preparations 2006; the effect of microorganisms in the bioremediation of the eutrophic water body 2009).
Drawings
FIG. 1 shows the change trend of various indexes after treating water in example 2;
FIG. 2 is a diagram showing the effect of example 2 before treating a water body;
FIG. 3 is a diagram showing the effect of treating a water body in example 2;
FIG. 4 is a diagram showing the effect of example 3 before treating a water body;
FIG. 5 is a diagram showing the effect of example 3 after treating a water body.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. The specific embodiments described herein are merely illustrative of the invention and are not intended to be limiting.
The invention discloses a diversified microorganism system for improving eutrophic water and application thereof, and the specific embodiment is shown in the following examples.
Example 1 diversified microbial System for improving eutrophic Water body
The composite microbial system provided by the invention comprises a diversified microbial agent and an additive, wherein the microbial agent comprises bacillus subtilis, bacillus megaterium, bacillus laterosporus, lactic acid bacteria, nitromonas, bacillus licheniformis, trichoderma viride, saccharomyces cerevisiae, pseudomonas fluorescens, rhodospirillum rubrum, rhodopseudomonas palustris, rhodobacter sphaeroides and pseudomonas putida. The mass ratio of each strain in the system is as follows:
5-10 parts of a bacillus subtilis (ACCC 11060) bacterial liquid; 5-10 parts of bacillus megaterium (ACCC 11107) bacterial liquid; 3-5 parts of a brevibacillus laterosporus (AMCC 100017) bacterial liquid; 1-3 parts of lactobacillus (ACCC 11016) bacterial liquid; 3-5 parts of nitromonad (CGMCC 9412) bacterial liquid; 5-10 parts of bacillus licheniformis (ACCC 10236) bacterial liquid; 1-3 parts of a green wood bacterium (CGMCC10922) bacterial liquid; 1-3 parts of saccharomyces cerevisiae (CICC 32355) bacterial liquid; 1-3 parts of pseudomonas fluorescens (ACCC 01047) bacterial liquid; 1-3 parts of rhodospirillum rubrum (ATCC 27048) bacterial liquid; 1-3 parts of rhodopseudomonas palustris (BNCC 227955) bacterial liquid; 3-5 parts of spherical rhodobacter (CGMCC 12310) bacterial liquid; 3-5 parts of pseudomonas putida (AS1.1003) bacterial liquid.
The preparation method of the microbial inoculum comprises the following steps:
separately culturing the above single bacteria at constant temperature of 30 deg.C under shaking at 200rpm in a shaking box to the same order of magnitude of 106-108CFU/ml. The culture medium comprises the following components in percentage by mass: 5-10% of soybean peptone; 2.5 to 5 percent of glucose; 0.5 to 1 percent of sodium chloride; 0.5 to 1 percent of calcium chloride; 0.5 to 1 percent of magnesium sulfate; 0.5 to 1 percent of potassium hydrogen phosphate.
The additive is composed of starch, diatomite, calcium carbonate and compound amino acid, and is in a powder shape. The mass ratio of starch, diatomite, calcium carbonate and compound amino acid in the additive is (1-2): (3-5): (3-5): (0.2-0.5). The compound amino acid comprises lysine, proline, glycine, tryptophan and glutamic acid. The mass ratio of the compound amino acid is (5-7): (0.05-0.15): (5-7): (0.05-0.15): (0.5-1.5).
The preparation method of the additive comprises the following steps: the starch, the diatomite, the calcium carbonate and the compound amino acid in the additives with specific mass are uniformly mixed to obtain a mixture.
The method for mixing the microorganism and the additive comprises the following steps: and mixing the mixed microbial inoculum prepared in the step and an additive in proportion to obtain a carrier attached with the compound microorganisms, and then airing, stirring and crushing the carrier into powder. The proportion of the mixed microbial inoculum and the additive is (1-2L): (50-100 kg).
Example 2 application example of diversified microorganism System
The river reach is about 600m, before treatment, the average river width of the river reach is about 10 m, the fluidity is poor, the self-cleaning capability is weak, the water quality of the river reach is dark green, flocculent floaters are arranged on the river surface, the part is seriously black and smelly, the smell is sharp, and the sense is extremely poor. Several main water quality indexes are inferior to the standard limit value of surface water V-type water, and the specific parameters are shown in the following table.
| Index (I) | Measured value | Class V water standard | Over limit |
| Total phosphorus | 0.86 | 0.40 | 2.15 times of |
| Total nitrogen | 13.40 | 2.00 | 6.70 times of |
| Ammonia nitrogen | 12.20 | 2.00 | 6.10 times of |
| COD | 23.00 | 40.00 | Not exceeding standard |
| Transparency | 32.00 |
The microbial product is put in the water body in a dosage of 1.5 g per cubic meter at the beginning of 10 months, and the microbial product is put in the water body once a month. 45 kg of microbial product was used for the whole treatment cycle. After half a year of compound microorganism putting, all indexes show obvious better trend, and the water quality of the monitored cross section is improved from poor V type to IV type before treatment by 4 months and 17 days. The change trend of each index is shown in figure 1, and the water surface effect before and after treatment is shown in figures 2 and 3.
Example 3 application example of diversified microorganism System
The body of remedial water is a lake of about 0.3 square kilometers. Before treatment, the aquatic weeds and algae are overflowed to cause disasters, and the water body smells in summer. Before treatment, the total phosphorus content is 0.17mg/L, the organic matter content is 40%, and the depth of the sludge is 50 cm. During the treatment period, 1.5 g of microbial product/cubic meter of water is put in, and the microbial product is added once a month. After four months of compound microorganism feeding, the total phosphorus content, the organic matter content and the sludge depth are obviously improved and respectively reduced to 0.06mg/L, 15.6 percent and 23 cm. The water surface effect before and after treatment is shown in figures 4 and 5.
The two experimental data of the embodiment 2 and the embodiment 3 show that the diversified composite microbial system can still effectively treat eutrophic organic pollution under the condition that the pollution degree, the geographical position and the pollution projects are different; can solve the problems of weak adaptability and unstable effect of the existing microorganism water purification technology in different water qualities.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (2)
1. A diversified microorganism system for improving eutrophic water is characterized in that: consists of a plurality of microbial inoculum and additive,
the diversified microbial inoculum consists of the following components in parts by mass: 5-10 parts of bacillus subtilis liquid; 5-10 parts of bacillus megaterium liquid; 3-5 parts of a brevibacillus laterosporus bacterial liquid; 1-3 parts of lactobacillus liquid; 3-5 parts of nitromonas bacterium liquid; 5-10 parts of bacillus licheniformis liquid; 1-3 parts of a green wood fungus liquid; 1-3 parts of saccharomyces cerevisiae bacterial liquid; 1-3 parts of pseudomonas fluorescens bacterial liquid; 1-3 parts of rhodospirillum rubrum liquid; 1-3 parts of rhodopseudomonas palustris bacterial liquid; 3-5 parts of spherical rhodobacter bacteria liquid; 3-5 parts of pseudomonas putida bacterial liquid;
the additive consists of starch, diatomite, calcium carbonate and compound amino acid, wherein the mass ratio of the starch to the diatomite is (1-2): (3-5): (3-5): (0.2-0.5);
the dosage proportion of the diversified microbial inoculum and the additive is (1-2L): (50-100 kg);
the order of magnitude of thalli in each bacterial liquid of the diversified microbial inoculum is 106-108CFU/mL；
The culture medium for culturing each strain in the diversified microbial inoculum consists of the following components in percentage by mass: 5-10% of soybean peptone; 2.5 to 5 percent of glucose; 0.5 to 1 percent of sodium chloride; 0.5 to 1 percent of calcium chloride; 0.5 to 1 percent of magnesium sulfate; 0.5 to 1 percent of potassium hydrogen phosphate;
the mixing method of the diversified microbial inoculum and the additive comprises the following steps: mixing the prepared mixed microbial inoculum and additives in proportion to obtain a carrier attached with the compound microorganisms, and then airing, stirring and crushing the carrier into powder.
2. The use of a diverse microbial system for the amelioration of eutrophic water bodies as defined in claim 1 in the treatment of water bodies, said use being embodied in the reduction of organic matter content in water bodies, or sludge depth in river and lake systems.
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| CN102701460A (en) * | 2012-07-08 | 2012-10-03 | 中国人民大学 | Method for processing starch waste water and realizing resourcezation of waste water by one photosynthetic bacterium strain |
| CN104724883A (en) * | 2015-02-13 | 2015-06-24 | 青岛市畜牧兽医研究所 | Method for recycling nitrogen and phosphorus in sewage |
| CN105331565A (en) * | 2015-12-15 | 2016-02-17 | 孙娟 | Microbial agent for purifying sanitary sewage and preparation method of microbial agent |
| CN105461082A (en) * | 2016-01-06 | 2016-04-06 | 中国人民大学 | Method for processing and recycling soybean processing wastewater through photosynthetic bacteria |
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