CN104724883A - Method for recycling nitrogen and phosphorus in sewage - Google Patents
Method for recycling nitrogen and phosphorus in sewage Download PDFInfo
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- CN104724883A CN104724883A CN201510076205.2A CN201510076205A CN104724883A CN 104724883 A CN104724883 A CN 104724883A CN 201510076205 A CN201510076205 A CN 201510076205A CN 104724883 A CN104724883 A CN 104724883A
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Abstract
The invention discloses a method for recycling nitrogen and phosphorus in sewage. The method comprises the following steps: 1) draining sewage into a settling basin through an underground sewage drain pipeline net, separating out a solid part and a fluid part through solid-liquid separation and submerged pump equipment; and 2) enabling the fluid part obtained in the step 1) to flow into a sewage anaerobic fermentation processing system, thereby obtaining biogas fluid, wherein the obtained biogas fluid can be used for duckweed aquaculture in indoor algae ponds; collecting duckweed, drying and crushing the collected duckweed to obtain duckweed dry powder, wherein the obtained duckweed dry powder can be used for producing industrial alcohol. According to the method provided by the invention, through anaerobic fermentation and duckweed aquaculture, nitrogen and phosphorus, which are harmful to the environment, in sewage are fast converted into useful resources to the maximum extent, the obtained economic benefit are beneficial to further promotion of the work of environment protection and realize virtuous cycle.
Description
Technical field
The present invention relates to environment protection and technical field of resource utilization, a kind of method that nitrogen phosphor resourceization utilizes in sewage.
Background technology
Along with the continuous aggravation of energy demand, environmental stress and Food Security, increasing researchist starts to be conceived to the novel substitutability clean biometric energy of R and D and new feed raw material.Follow the Bioenergy development strategy of " not striving grain with people; do not strive ground with grain ", the Corn Fuel Ethanol plan of the U.S. and the sugarcane ethanol plan of Brazil are not suitable for the present situation that China has a large population and a few land, and energy organism and the feed resource of finding also development of new have great strategic importance and using value.
At present, China's protein feed material inlet is more than 70%, and annual imported soybean about 4,000 ten thousand tons, fish meal 1,200,000 tons and other protein feed hundreds of thousands of tons are the importer of last resorts of global protein raw material.Though China's phosphate rock resource reserves are comparatively large, skewness, grade are lower, according to mining industry association, if only utilize grade to be greater than the rich ore of 30%, only can meet to needs in 2028.In addition, sewerage nitrogen and phosphor excessive emissions not only causes severe contamination to environment, and the waste of the protein feed resources causing China in short supply and non-renewable phosphate rock resource.
Sewage is source of pollution, is again valuable resource, and it comprises the necessary various nutritive ingredient of plant.But these nutritive ingredient majorities exist with the form of organic macromolecule, need to be absorbed by waterplant by the slow degraded of environmental microorganism.And anaerobic fermentation system can realize the decomposition to organic macromolecule in sewage within a short period of time, thus accelerate waterplant absorbing these nutritive ingredients, realize the fast purification of sewage.
The distinguishing features such as duckweed is the monocotyledons that blooms minimum in the world, has fast growth, and starch, protein content are high, duckweed can directly utilize sewage to carry out biomass growth simultaneously.The duckweed of adapted varieties is not only stronger to the removal ability of Water, phosphorus, and there is certain tolerance, be applicable to carry out restoration of the ecosystem and the purifying treatment to the higher waste water of the content of nitrogen and phosphorous such as sanitary sewage, aquaculture wastewater to eutrophication water and other natural waters.Therefore, duckweed has environment protection and new exploitation of energy resources two kinds of purposes simultaneously.
Summary of the invention
The object of this invention is to provide the method for nitrogen in a kind of sewage, phosphor resourceization utilization.The present invention utilizes this novel method that the nitrogen phosphorus in sewage is transformed into available resource, turns waste into wealth, and has important commercial application and is worth.
The object of the present invention is achieved like this:
Sewage enters sedimentation by underground blowdown pipe network, by solid-liquid separation, submersible pump equipment, isolate liquid portion and enter anerobic sowage fermentative processing system, the efficient anaerobic bacterium utilizing this system decomposes rapidly the macromolecule organic in sewage at short notice, make the elements such as nitrogen wherein, phosphorus free out with small molecules form, thus improve the assimilated efficiency of duckweed.Fermentation after produce natural pond liquid enter indoor duckweed cultivating pool, nitrogen wherein, phosphorus absorb by duckweed.Gained duckweed dry powder rich in starch and protein, by fermentation by saccharomyces cerevisiae manufacture alcohol.
By above process, realize the recycling of nitrogen in sewage, phosphorus.This system cloud gray model flow process is shown in Fig. 1.
Specifically, the invention provides and a kind of nitrogen phosphorus in sewage is carried out the method for recycling, wherein the method comprises the following steps:
1) sewage enters sedimentation by underground blowdown pipe network, by solid-liquid separation, submersible pump equipment, isolates solid part and fluid section;
2) step 1) fluid section of gained enters anerobic sowage fermentative processing system, and the natural pond liquid of acquisition is used for the duckweed cultivation in indoor algae pond, and collect duckweed, dry, pulverize, gained duckweed dry powder is used as the production of industrial spirit.
Further, step 1) through the sedimentation time of sedimentation be 36-54 hour.
Further, step 2) anerobic sowage fermentative processing step be: in fluid, add the NaOH that mass volume ratio is 2%, salt adding acid for adjusting pH to 7.2 after standing 36h, drop in anerobic sowage fermentor tank, take anaerobic activated sludge as inoculum, be the inoculum size inoculation of 30% by volume, form anaerobic fermented liquid, then 8% access composite bacteria liquid of the overall accumulated amount of anaerobic fermented liquid is pressed, 20-30 DEG C of anaerobically fermenting 40 hours; Bacterium in described composite bacteria liquid is made up of Cellulomonase fimi, Rhodopseudomonas palustris, trichoderma viride and yeast.
Further preferably, by thalline number content meter: Cellulomonase fimi 30% in described composite bacteria liquid, Rhodopseudomonas palustris 45%, trichoderma viride 17%, yeast 8%.
Further, step 2) described in indoor algae pond, the temperature of its indoor remains on 25-30 DEG C.
Further, step 2) described in duckweed cultivation step be: anerobic sowage fermentor tank treatment system obtain natural pond liquid enter Buffer Pool, successively obtain water purification through No. 1 duckweed culturing pool 5 days, No. 2 duckweed culturing pools 5 days, No. 3 duckweed culturing pools after 5 days, gained water purification indices meets " urban wastewater treatment firm pollutant emission standard ", can to environmental emission.This system cloud gray model flow process is shown in Fig. 2.
The present invention is after indoor algae pool duckweed of park cultivates 15 days, and the specific absorption of duckweed to natural pond liquid total nitrogen reaches 74.5%, reaches 94.4% to the specific absorption of natural pond liquid total phosphorus; In duckweed dry-matter, starch content is up to 52-54%, protein content up to 12-13%, by fermentation by saccharomyces cerevisiae manufacture alcohol.Shown by fermentation test, using Wine brewing yeast strain CCTCC M206111 as fermented bacterium, final ethanol fermentation efficiency reaches 89.3%-91.5%, alcohol production rate reaches 2.15-2.25g/L/h, and this illustrates that the duckweed utilizing the inventive method to cultivate has the using value of manufacture alcohol.Therefore, another object of the present invention is to provide a kind of industrial use, that is: the duckweed dry powder prepared of aforesaid method is by the purposes of yeast saccharomyces cerevisiae in fermentative production industrial spirit.Preferably, described yeast saccharomyces cerevisiae is bacterial strain CCTCCM206111.
Compared with prior art, the present invention has screened efficient composite anaerobic bacterium, decompose rapidly the macromolecule organic in sewage at short notice, make the elements such as nitrogen wherein, phosphorus free out with small molecules form, thus improve the assimilated efficiency of duckweed, 74.5% is reached to the specific absorption of natural pond liquid total nitrogen, 94.4% is reached to the specific absorption of natural pond liquid total phosphorus; In addition, gained duckweed dry powder rich in starch and protein, starch content up to 52-54%, protein content up to 12-13%, by fermentation by saccharomyces cerevisiae manufacture alcohol.In a word, the present invention is by anaerobically fermenting and duckweed cultivation, and to greatest extent nitrogen environmentally harmful in sewage, phosphorus are quickly converted to useful resource, the economic benefit obtained will contribute to promoting environmental protection work further, realize benign cycle.
Accompanying drawing explanation
Fig. 1 is that sewage recycling utilizes schematic flow sheet;
Fig. 2 is duckweed sewage disposal operational scheme schematic diagram.
Embodiment
Below by embodiment, and by reference to the accompanying drawings, detailed explanation is made to technical scheme of the present invention.Embodiments of the invention all illustrate effect of the present invention for the process of town sewage, and certainly, the present invention is equally applicable to the process that other are rich in nitrogen-phosphorus wastewater.
The technology used in following examples, unless stated otherwise, is routine techniques known to those skilled in the art; The plant and instrument used, reagent etc., only this specification sheets special instruction, be that the research of this area and technician can be obtained by public approach.Few root duckweed picks up from Duan Bolan town, Jimo City of Shandong Province, and sewage picks up from Licang District, Qingdao of Shandong province town dweller's sanitary sewage.
Embodiment 1 one kinds utilizes the method for nitrogen phosphorus in sewage fast by anaerobically fermenting and duckweed cultivation
Sewage treatment process as shown in Figure 1 and Figure 2.First, building a volume is 100 cubic metres, structure is the sedimentation that brick mixes, sewage enters No. 1 sedimentation by underground blowdown pipe network, after precipitation 48 hours, lower floor's solid part is used as fertilizer, supernatant liquid part adds the NaOH that mass volume ratio is 2%, salt adding acid for adjusting pH to 7.2 after standing 36h, enter anerobic sowage fermentor tank treatment system (UASB anaerobic reactor, high 8 meters, diameter 3 meters, useful volume 56 cubic metres, can disposablely dispose of sewage 5 tons, purchased from Qingdao Yiweiyou Environment Engineering Equipment Co., Ltd.), take anaerobic activated sludge as inoculum, be the inoculum size inoculation of 30% by volume, form anaerobic fermented liquid, then 8% access composite bacteria liquid of the overall accumulated amount of anaerobic fermented liquid is pressed, 20-30 DEG C of anaerobically fermenting 40 hours, by thalline number content meter: Cellulomonase fimi 30% in composite bacteria liquid, Rhodopseudomonas palustris 45%, trichoderma viride 17%, yeast 8%.After fermentation ends, gained cripple is for processing fertilizer, and natural pond liquid is used for the few root duckweed of closed algae pond culture, and biogas is used as sewage disposal base domestic fuel.Natural pond liquid enters enclosed indoor duckweed cultivating pool, room temp remains on 25-30 DEG C, step is: the natural pond liquid that anerobic sowage fermentor tank treatment system obtains enters Buffer Pool, successively through No. 1 duckweed culturing pool 5 days, No. 2 duckweed culturing pools 5 days, No. 3 duckweed culturing pools after 5 days, water purification is obtained after nitrogen phosphorus etc. is wherein absorbed purification by few root duckweed, gained water purification indices meets " urban wastewater treatment firm pollutant emission standard ", can to environmental emission.Gained duckweed is placed in the drying in oven 24h of 75 DEG C, pulverizes, and after crossing 35 mesh sieves, hermetically drying is preserved.
Embodiment 2 duckweed measures the assimilated efficiency of nitrogen phosphorus in the liquid of natural pond
In embodiment 1, after the process of anerobic sowage fermentor tank treatment system, gained natural pond liquid is used for the test of the present embodiment.Employing square plastic feed basin (long 20cm, wide 15cm, high 10cm), as test chamber, adds 1000mL for examination natural pond liquid, then will add fresh weight 2g in respective process by cultured few root duckweed in advance, maintains 15 days.20 repetitions are established in often kind of duckweed process, and with the water sample not adding duckweed for contrast, if 5 repetitions.All process available light uniformity place in laboratory cultivates, observes.Experiment indoor air temperature maintains 25-30 DEG C.Duration of test, within every 3 days, get 3 repetitions respectively at random to two kinds of duckweed process, control group also gets 3 repetitions, residue is repeated to change 1000mL former in examination sewage simultaneously.Repeat to fish for whole duckweed to got each process, dry and weigh and survey nitrogen and phosphorus content in body, measure the volume of each process and contrast water sample, total nitrogen and total phosphorus.
In water, total nitrogen adopts alkaline chitinase to be oxidized a ultraviolet spectrophotometry, and in water, total phosphorus adopts alkaline potassium per-sulfate digestion one molybdenum-antimony anti-spectrophotometric method, and in duckweed body, full nitrogen adopts H
2sO
4-H
2o
2-diffusion process, full phosphorus adopts H
2sO
4-H
2o
2-molybdenum antimony resistance colorimetric method.Test-results calculates gets 3 mean values repeated, and in water, total nitrogen and total phosphorus removal amount add up removal amount with the unit surface water surface and represent, duckweed absorbed nitrogen phosphorus amount also adds up absorbed dose with the unit surface water surface and represents.
Test-results shows, in water, total nitrogen and total tp removal rate reach 85.3% and 95.7%, wherein removed total nitrogen and total phosphorus have respectively 87.4% and 98.6% absorb by duckweed.Therefore, the specific absorption of duckweed to total nitrogen reaches 74.5%, reaches 94.4% to the specific absorption of total phosphorus, and the most of the nitrogen phosphorus in the liquid of natural pond can be absorbed by duckweed.
The mensuration of starch and protein content in the duckweed of embodiment 3 natural pond liquid cultivation
Utilize the content of total sugar content method (starch content=total sugar content × 0.909) indirect measurement starch.Embodiment 1 gained duckweed dry powder in boiling water bath by 1.2M hydrochloric acid hydrolysis.Use 10M NaOH that pH value is adjusted to 7.0, add plumbic acetate precipitating proteins.Use C18 solid-phase extraction column filtering supernatant, use HPLC (Thermo 2795, Thermo Corp., USA) analyze hydrolysis reaction, detector uses light scattering detector (All-Tech ELSD 2000, All-tech., Corp., USA).Detailed process is see document Zhang, L., Zhao, H., Gan, M., Jin, Y., Gao, X., Chen, Q., Guan, J., Wang, Z., 2011.Application of simultaneous saccharification and fermentation (SSF) fromviscosity reducing of raw sweet potato for bioethanol production at laboratory, pilot andindustrial scales.Bioresour.Technol.102,4573-4579.)
Crude protein content uses Kjeldahl determination principle (Gerhardt Corp., Germany) to measure.Sample pretreatment process see document Thiex, N.J., Manson, H., Anderson, S., Persson, J.A., 2002.Determination ofcrude proteinin animal feed, forage, grain, and oilseeds by using block digestion with a copper catalyst andsteam distillation into boric acid:collaborative study.J.AOAC Int.85,309-317. duckweed dry powder uses H
2sO
4digestion, is transformed into N (NH4)
2sO4, adds K
2sO4 (Cu is as catalyzer) improves boiling point with fast reaction speed.Use alkaline water vapor distillation method release ammonia, and quantize the content of N with standard acidimetry.Aluminium alloy heater is used to improve digestive efficiency.
Measurement result shows, and in duckweed dry powder, starch content is up to 52-54%, and crude protein content reaches 12-13%.As can be seen here, the duckweed that the inventive method cultivates can be used as the high quality raw material of energy grade starch and feed protein matter.
The efficiency checking of embodiment 4 duckweed fermentative production industrial spirit
Wine brewing yeast strain CCTCC M206111 is used for duckweed fermentation.This bacterial strain is preserved on the YPD gel entrapment culture base of 4 DEG C, this culture medium prescription: 1% yeast extract, 2% peptone, 2% glucose, 1.5% agar.Inoculating strain in 250mL Erlenmeyer flask, add in container sterilising medium 100mL (containing glucose 10g, yeast extract 0.85g, ammonium sulfate 0.13g, MgSO
47H
2o 0.01g, CaCl
20.006g).Use 2M hydrochloric acid or 2M NaOH that Medium's PH Value is adjusted to 6.0.Embodiment 1 gained duckweed dry powder carries out pre-treatment and fermentation test with the solid-to-liquid ratio of 1: 5-1: 7.All substratum equal autoclaving 15 minutes under the condition of 120 DEG C before use.
α-amylase (the Liquozyme Supra of 90KNU/mL, Novi believes, Beijing, China) and saccharifying enzyme (the Amylase AG of 300AGU/mL, Novi believes, Suzhou is Chinese) are for the liquefaction of duckweed dry powder and saccharification.The liquefaction of duckweed dry powder processes 10 minutes under 94-96 DEG C of condition, and the dry powder of liquefaction autoclaving 15 minutes under the condition of 120 DEG C, is cooled to room temperature.Mashing carries out in 250mL Erlenmeyer flask, adds the dry powder of 100g, action condition be pH6.0,50 DEG C two hours.Saccharifying enzyme adds according to the dosage of every gram of dry powder 1.5 AGU.
In 250mL Erlenmeyer flask, add 100 grams of pretreated duckweed mud, carry out fermentation test.In this Erlenmeyer flask, add the yeast-inoculated thing of 8%-12%, 29-31 DEG C, cultivate under 200-250rpm condition.Sample regularly reclaims, to analyze the concentration of ethanol and the residual of sugar.The concentration of ethanol uses vapor-phase chromatography to detect, and gas chromatograph uses U.S. Agilent 7820A, configuration flame ionization detector and stainless steel pillar.Ethanol fermentation efficiency and productivity are calculated as follows:
Ethanol × 100% of the ethanol that fermentation efficiency=fermentation produces/can produce in theory
Alcohol production rate=ethanol final concentration/fermentation time
Test-results shows, and ethanol fermentation efficiency reaches 89.3%-91.5%, and alcohol production rate reaches 2.15-2.25g/L/h.This explanation utilizes the duckweed of fish-farming with sewage to have the using value of manufacture alcohol.
Above embodiment is only not used in for illustration of the present invention and limits the scope of the invention.After having read the content that the present invention describes, the adjustment of the various unsubstantialities that those skilled in the art are done on the basis of a method according to the invention and amendment, its technical scheme has belonged to the scope of the application's appended claims protection equally.
Claims (8)
1. nitrogen phosphorus in sewage is carried out a method for recycling, it is characterized in that, the method comprises the following steps:
1) sewage enters sedimentation by underground blowdown pipe network, by solid-liquid separation, submersible pump equipment, isolates solid part and fluid section;
2) step 1) fluid section of gained enters anerobic sowage fermentative processing system, and the natural pond liquid of acquisition is used for the duckweed cultivation in indoor algae pond, and collect duckweed, dry, pulverize, gained duckweed dry powder is used as the production of industrial spirit.
2. according to claim 1 nitrogen phosphorus in sewage is carried out the method for recycling, it is characterized in that, step 1) through the sedimentation time of sedimentation be 36-54 hour.
3. according to claim 1 nitrogen phosphorus in sewage is carried out the method for recycling, it is characterized in that, step 2) anerobic sowage fermentative processing step be: in fluid, add the NaOH that mass volume ratio is 2%, salt adding acid for adjusting pH to 7.2 after standing 36h, drop in anerobic sowage fermentor tank, take anaerobic activated sludge as inoculum, be the inoculum size inoculation of 30% by volume, form anaerobic fermented liquid, then 8% access composite bacteria liquid of the overall accumulated amount of anaerobic fermented liquid is pressed, 20-30 DEG C of anaerobically fermenting 40 hours; Bacterium in described composite bacteria liquid is made up of Cellulomonase fimi, Rhodopseudomonas palustris, trichoderma viride and yeast.
4. according to claim 3 nitrogen phosphorus in sewage is carried out the method for recycling, it is characterized in that, by thalline number content meter: Cellulomonase fimi 30% in described composite bacteria liquid, Rhodopseudomonas palustris 45%, trichoderma viride 17%, yeast 8%.
5. according to claim 1 nitrogen phosphorus in sewage is carried out the method for recycling, it is characterized in that, step 2) described in indoor algae pond, the temperature of its indoor remains on 25-30 DEG C.
6. according to claim 1 nitrogen phosphorus in sewage is carried out the method for recycling, it is characterized in that, step 2) described in duckweed cultivation step be: anerobic sowage fermentor tank treatment system obtain natural pond liquid enter Buffer Pool, successively obtain water purification through No. 1 duckweed culturing pool 5 days, No. 2 duckweed culturing pools 5 days, No. 3 duckweed culturing pools after 5 days, gained water purification indices meets " urban wastewater treatment firm pollutant emission standard ", can to environmental emission.
7. duckweed dry powder according to claim 1 is by the purposes of yeast saccharomyces cerevisiae in fermentative production industrial spirit.
8. purposes according to claim 7, is characterized in that, described yeast saccharomyces cerevisiae is bacterial strain CCTCCM206111.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105541074A (en) * | 2015-12-11 | 2016-05-04 | 美的集团股份有限公司 | Method and system for treating waste |
| CN107840527A (en) * | 2017-04-21 | 2018-03-27 | 湖南大学 | It is a kind of to utilize dilute floating pulse duckweed and the technique and method of colloid Rhodopseudomonas Combined Treatment organic wastewater |
| CN110128181A (en) * | 2019-06-14 | 2019-08-16 | 武汉益锦祥生物环保有限公司 | A kind of production method and process units of the phycomycete fertilizer based on biogas slurry |
| CN110904089A (en) * | 2019-12-23 | 2020-03-24 | 清水华明(武汉)生态科技有限公司 | Diversified microorganism system for improving eutrophic water body and application thereof |
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| CN105541074A (en) * | 2015-12-11 | 2016-05-04 | 美的集团股份有限公司 | Method and system for treating waste |
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| CN107840527B (en) * | 2017-04-21 | 2020-12-15 | 湖南大学 | Method for treating organic wastewater by using lemna paucicostata and pseudomonas glii in combined manner |
| CN110128181A (en) * | 2019-06-14 | 2019-08-16 | 武汉益锦祥生物环保有限公司 | A kind of production method and process units of the phycomycete fertilizer based on biogas slurry |
| CN110904089A (en) * | 2019-12-23 | 2020-03-24 | 清水华明(武汉)生态科技有限公司 | Diversified microorganism system for improving eutrophic water body and application thereof |
| CN110904089B (en) * | 2019-12-23 | 2020-09-01 | 清水华明(武汉)生态科技有限公司 | Diversified microorganism system for improving eutrophic water body and application thereof |
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