CN110882169A

CN110882169A - Dissolving mask for whitening skin and removing freckles and preparation method thereof

Info

Publication number: CN110882169A
Application number: CN201911414552.6A
Authority: CN
Inventors: 陈常军
Original assignee: Changsha Ruyang Environmental Protection Technology Co Ltd
Current assignee: Changsha Ruyang Environmental Protection Technology Co Ltd
Priority date: 2019-12-31
Filing date: 2019-12-31
Publication date: 2020-03-17

Abstract

The invention provides a dissolving facial mask for whitening and removing freckles and a preparation method thereof. The facial mask obtained by the invention is a dissolving facial mask, and the facial mask can be gradually dissolved and absorbed by skin when in use.

Description

Dissolving mask for whitening skin and removing freckles and preparation method thereof

Technical Field

The invention relates to the technical field of beauty and skin care, in particular to a dissolving facial mask for whitening and removing freckles and a preparation method thereof.

Background

Whitening is a permanent topic for most women, and besides the overall darkness of skin caused by melanin deposition, color spots are also a big road barrage on the way of whitening and protecting skin, so the two skin-protecting problems of whitening and freckle removal are always needed to be solved simultaneously. The mask product is certainly a good choice.

The facial mask is a skin care product which is coated or applied on the surface of facial skin of a human body, is removed, scrubbed or kept after a period of time and plays a role in centralized nursing or cleaning. The mask can be classified into a non-base cloth type and a base cloth type according to the use mode.

At present, most of facial masks sold in the market are of a base cloth type, and the facial masks take fiber base cloth as a carrier and are supplemented with nutrient solutions with different effects, so that the absorption speed and the absorption amount of skin on nutrient components are increased, and the skin care effect is rapidly improved. The main components of the nutrient solution are extracted from effective components of plants, animals or natural minerals, and the nutrient solution is prepared by adopting corresponding processes and technologies for extraction and concentration, and has the main functions of whitening, moisturizing, providing nutrition and moisture required by skin and delaying skin aging. The base cloth type facial mask mostly uses non-woven cloth as base cloth, and the processing technology comprises hot rolling, spun bonding, spunlace, melt-blown and the like, wherein the spunlace non-woven cloth is most applied, and the facial mask can carry a large amount of nutrient solution and ensure full absorption of skin because the facial mask has soft hand feeling, humane texture, high porosity and good moisture retention. The commercially available non-woven mask base cloth mainly takes cotton fibers, viscose fibers, cuprammonium fibers and wood pulp fibers as main raw materials, and a few products adopt real silk fibers, seaweed fibers and the like. Many base cloths use adhesives during the manufacturing process, which may cause skin irritation or other adverse reactions. Moreover, no matter how the breathability of the base fabric is improved, there is no way to achieve substantial improvement, and the long application time will in turn absorb skin moisture.

As for the non-base cloth type, a relatively explosive Danggu sleep mask is sold in the market at present, the sleep mask is a jelly type, is usually jelly or cream, and utilizes a thick layer of essence dressing to block the contact of facial skin and air, block the evaporation of skin moisture, increase the humidity of stratum corneum and soften cutin; the temperature of the epidermis rises to expand capillary pores, which is beneficial to the smooth absorption of nutrient components. However, the so-called sleeping mask does not need to be cleaned, and actually, the sleeping mask needs to be cleaned within 6-8 hours of use so as to prevent the protective film of the dry glue from preventing the normal breathing and metabolism of the skin and playing a role in the adverse reaction.

Moreover, even if the whitening and freckle-removing components are added, the whitening and freckle-removing components are difficult to penetrate through the horny layer to act on the corium layer in the using process, so that the skin state cannot be fundamentally improved, and the whitening and freckle-removing effects are achieved.

Disclosure of Invention

The invention aims to provide a dissolving mask for whitening and removing freckles and a preparation method thereof, and aims to solve the technical problems of poor air permeability, complicated use steps, poor whitening and freckle removing effects and the like.

In order to achieve the purpose, the invention provides a preparation method of a dissolving mask for whitening and removing freckles, which comprises the following specific steps:

(1) adding micromolecular sodium alginate into the bletilla striata extracting solution, stirring until the micromolecular sodium alginate is completely dissolved, then adding fresh bitter gourd juice, stirring and uniformly mixing, then adding micromolecular chondroitin sulfate and micromolecular silk fibroin, uniformly oscillating by ultrasonic waves to obtain a mixed solution, and then performing modification treatment by using transmembrane peptide to obtain a first phase;

(2) then dissolving the phytosterol in denatured ethyl acetate to obtain a second phase;

(3) then slowly dripping the first phase into the second phase while stirring, continuously stirring until the first phase is completely mixed after dripping is finished, and standing for defoaming or vacuum defoaming to obtain a spinning solution;

(4) finally, carrying out electrostatic spinning on the spinning solution to obtain a fiber membrane, cutting according to the shape of the required facial mask, sterilizing and packaging to obtain the dissolved facial mask for whitening and removing the freckles;

wherein the weight average molecular weight of the small-molecule chondroitin sulfate, the small-molecule silk fibroin and the small-molecule sodium alginate is below 5 kDa; the modified ethyl acetate is prepared by mixing ethyl acetate with butanediol, L-malic acid and disodium glycyrrhizinate.

Preferably, the mass ratio of the small-molecule chondroitin sulfate, the small-molecule silk fibroin, the fresh bitter gourd juice, the small-molecule sodium alginate, the bletilla striata extracting solution, the cell-penetrating peptide, the phytosterol and the modified ethyl acetate is 1: 3-5: 8-10: 12-15: 6-8: 5-8: 2-4: 8-10; the bletilla striata extracting solution is obtained by extracting dried bletilla striata with water 15-20 times of the weight of the dried bletilla striata and performing enzymolysis.

Preferably, in the step (1), the preparation method of the small molecule sodium alginate comprises the following steps: adding 1 part by weight of macromolecular sodium alginate into 70-80 parts by weight of deionized water at 75-80 ℃, stirring until the macromolecular sodium alginate is completely dissolved, then adding 0.4-0.6 part by weight of hydrogen peroxide, stirring and reacting at 60-70 ℃ for 20-25 hours, and carrying out alcohol precipitation to obtain the micromolecular sodium alginate; the weight average molecular weight of the macromolecular sodium alginate is 300-500 kDa.

Further preferably, the alcohol precipitation is carried out by adding absolute ethyl alcohol twice the volume of the reaction solution, precipitating, and centrifuging.

Preferably, in the step (1), the preparation method of the bletilla striata extract comprises the steps of crushing dry bletilla striata to prepare bletilla striata powder, transferring the bletilla striata powder into a pressure difference extractor, condensing the bletilla striata powder at the temperature of 2-7 ℃, vacuumizing the pressure difference extractor to-50 to-100 MPa within 20-50 seconds, keeping the pressure difference extractor for 2-5 minutes, spraying deionized water at the temperature of 15 ℃ for extraction, introducing purified compressed air, increasing the pressure in the pressure difference extractor to 0MPa within 1-3 minutes, continuing introducing the purified compressed air to enable the pressure in the pressure difference extractor to reach 3MPa, keeping the pressure for 2-5 minutes, stopping pressurizing, vacuumizing again to enable the pressure in the pressure difference extractor to be reduced to 0MPa, repeating the vacuumizing to decompression process for a plurality of times, carrying out solid-liquid separation through centrifugation, filtering the centrifugal liquid to obtain filtrate, adding cellulase and β -glucanase into the filtrate, carrying out enzymolysis on the bletilla striata solution at the temperature of 45-50 ℃ and the pH of 4.5-5 for 50 minutes, and carrying out enzyme deactivation, wherein the dosage of the cellulase and the β -glucanase are respectively 3.5 thousandths in mass.

Preferably, in the step (1), the preparation method of the fresh balsam pear juice comprises the following steps: cleaning fresh fructus Momordicae Charantiae, removing pulp, cutting, and squeezing to obtain fresh fructus Momordicae Charantiae juice.

Preferably, in step (1), the preparation method of the small molecule chondroitin sulfate is as follows: adding 1 part by weight of high molecular weight shark cartilage chondroitin sulfate into 10-12 parts by weight of deionized water, stirring for dissolving, then adding 0.03-0.05 part by weight of hydrogen peroxide, stirring for reacting at 50-55 ℃ for 3-5 hours, filtering to obtain filtrate, adjusting the pH to 5-6, adding absolute ethyl alcohol to make the volume final concentration of the absolute ethyl alcohol to be 60-70%, precipitating crystals, centrifuging, and washing with absolute ethyl alcohol to obtain the micromolecular chondroitin sulfate.

Preferably, in the step (1), the preparation method of the small-molecule silk fibroin is as follows: according to parts by weight, 1 part of mulberry silk is subjected to degumming and hydrolysis treatment to obtain a silk fibroin solution, then 0.003-0.005 part of elastase is added, the reaction is carried out for 5-8 hours at 25-30 ℃, enzyme deactivation and alcohol precipitation are carried out, and the micromolecule silk fibroin is obtained.

Further preferably, the degumming method comprises the following specific steps: soaking mulberry silk in 4-6 times of sodium carbonate aqueous solution with mass concentration of 0.05-0.1%, boiling for 30-40 minutes while stirring, repeating for 3-4 times, taking out, and washing with deionized water for 2-3 times.

Further preferably, the specific method of hydrolysis is: and soaking the degummed mulberry silk in a potassium chloride solution with the mass concentration of 1-1.2% and the weight of 3-5 times of that of the degummed mulberry silk, and treating for 2-3 hours at the temperature of 130-140 ℃ and under the pressure of 0.5-0.6 MPa.

Further preferably, the specific method for alcohol precipitation is as follows: adding absolute ethyl alcohol with the volume twice that of the reaction solution, precipitating and centrifuging.

Preferably, in step (1), the specific method of modification treatment is: adding the cell-penetrating peptide into the mixed solution, then adding SPDP (3- (2-pyridinedimercapto) propionic acid N-hydroxysuccinimide ester) dissolved in dimethyl sulfoxide, and stirring and reacting for 10-15 hours under the protection of argon.

More preferably, the mass-to-volume ratio of the cell-penetrating peptide, the mixed solution, dimethyl sulfoxide and SPDP is 1 g: 60-85 mL: 5-6 mL: 0.01 to 0.03 g.

Preferably, in the step (2), the denatured ethyl acetate is prepared by the following method: adding 0.06-0.08 part of butanediol, 0.1-0.2 part of L-malic acid and 0.01-0.02 part of disodium glycyrrhizinate into 1 part of ethyl acetate by weight part, and carrying out ultrasonic oscillation for 30-50 minutes to obtain the modified ethyl acetate.

Preferably, in the step (3), the dropping time of the first phase is 50 to 70 minutes.

Preferably, in the step (4), the specific method of electrostatic spinning is as follows: the wire outlet is a No. 6 needle head, the voltage is 15-25 kV, the distance from the spray head to the receiver is 20-25 cm, the spray flow is 0.5-0.8 ml/h, and the relative humidity is lower than 40%.

Preferably, in step (4), ultraviolet disinfection is adopted.

The dissolving facial mask for whitening and removing the freckles is obtained by the preparation method.

The invention has the following beneficial effects:

the invention is characterized in that micromolecular sodium alginate, bletilla striata extracting solution, fresh bitter gourd juice, micromolecular chondroitin sulfate, micromolecular silk fibroin, cell penetrating peptide and the like are used as raw materials to prepare a first phase, phytosterol and denatured ethyl acetate are used as raw materials to prepare a second phase, then the first phase is dripped into the second phase to prepare spinning solution, finally, a fiber membrane is obtained through electrostatic spinning, and the fiber membrane is cut into a mask shape. The facial mask obtained by the invention is a dissolving facial mask, is directly pasted on the face after being taken out of a packaging bag, has good air permeability, is uniformly sprayed with a layer of warm water with the temperature of 35-40 ℃ on the surface of the facial mask by using a skin-care spraying pot, or is used in a bath room filled with warm water mist, the facial mask gradually dissolves (the dissolving time is less than 10 minutes) and is absorbed by the skin, the skin absorption mainly passes through keratinocytes or intercellular gaps, and a normal complete barrier is a substance with the molecular weight of below 15 KDa.

The water-soluble substances are not easy to be absorbed by the skin, and the invention adopts the fat-soluble second relative water-soluble first property to carry out inclusion, thereby being beneficial to the skin absorption. The second phase is obtained by dissolving phytosterol in denatured ethyl acetate. Wherein, the phytosterol has high permeability to skin, can keep skin surface moisture, promote skin metabolism, inhibit skin inflammation, prevent sunburn erythema and skin aging, and can guide other components to permeate into skin and adhere to skin surface. The modified ethyl acetate is prepared by mixing ethyl acetate with butanediol, L-malic acid and disodium glycyrrhizinate, the dissolution of phytosterol can be realized by utilizing the ethyl acetate, but the ethyl acetate is volatile and can take away skin moisture; on the other hand, the butanediol has moisture retention, the L-malic acid has oxidation resistance (anti-pigment oxidation), can be easily dissolved and adhered to dead cells in a dry scale shape, can remove speckles, has a whitening effect, and the disodium glycyrrhizinate has an anti-inflammatory and antibacterial effect, has a cleaning effect on the skin, can clean sebum and dirt on the surface layer of the skin, and is beneficial to opening pores of the skin to absorb other components more quickly.

The existing mask on the market also contains sodium alginate, but is basically some macromolecular sodium alginate, and the skin has water-wet feeling when being used, but is not durable because the water-wet feeling is not absorbed by the skin. The mask needs to be cleaned after use, and macromolecular sodium alginate which cannot be absorbed by the skin can block pores on the surface of the skin, breed bacteria and is harmful to the skin. The invention uses the micromolecular sodium alginate, can effectively increase the water retention of the skin, is completely absorbed by the skin, improves the immune protection effect of the skin, repairs color spots and has the whitening effect. The small-molecular chondroitin sulfate is easy to absorb water, has good water-locking capacity and good moisturizing effect, and moisturizing is the root of whitening, improves skin moisture and is beneficial to promoting the absorption of whitening components. The fresh loofah juice contains B vitamins for preventing skin aging, vitamin C for whitening skin and other ingredients, can protect skin, eliminate color spots and play a role in whitening and removing spots, and the charantin is beneficial to skin regeneration, repairs the color spots and plays a good role in whitening and removing spots. The bletilla striata is rich in bletilla striata colloid, starch, glucose, volatile oil, pituitous substances, anthraquinone derivatives and other chemical components, has the effects of whitening, removing freckles, astringing to stop bleeding, reducing swelling and promoting granulation, effectively balances skin and keeps skin moist. Can promote epithelial cell repair, and directly participate in the repair and metabolic processes of damaged tissues and cells. Especially, contained bletilla striata colloid, namely bletilla striata glucan, is considered to be a non-undetermined irritant in an immune system for a long time, macrophage activation is usually caused, and the macrophage activation causes a series of reactions, so that fibroblast and keratinocyte migration is caused firstly, epidermal growth factor (ECGF) and Angiogenesis Factor (AF) are generated to resist aging wrinkled skin, meanwhile, the generation of collagen and elastin is promoted, the skin condition is improved, and the whitening and freckle removing effects are fundamentally achieved.

The invention also introduces the cell-penetrating peptide, the cell-penetrating peptide with positive surface potential and the micromolecule silk fibroin with negative electricity, etc. are electrostatically attracted, so that the retention time on the surface of the skin is prolonged, and the skin has good capability of crossing the physiological barrier. Therefore, the cell-penetrating peptide further promotes the absorption of each component in the first phase, avoids the residue on the surface of the skin and avoids the cleaning trouble.

Hydroxyl in the micromolecular sodium alginate and the micromolecular chondroitin sulfate and amino, carboxyl and the like in the micromolecular silk fibroin form a hydrogen bond effect, so that the mechanical property of the mask is greatly enhanced, and the mask is prevented from being damaged due to pulling in use.

In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below.

Detailed Description

The following is a detailed description of embodiments of the invention, but the invention can be implemented in many different ways, as defined and covered by the claims.

The cell-penetrating peptide of the invention, type tat (48-60), was purchased from Wuhan Baiyixin Biotechnology GmbH; shark cartilage chondroitin sulfate (weight average molecular weight 10-30kDa) was purchased from Bolin Biotechnology, Inc., Shanxi.

Example 1:

a preparation method of a dissolving mask for whitening and removing freckles comprises the following specific steps:

The mass ratio of micromolecular chondroitin sulfate, micromolecular silk fibroin, fresh bitter gourd juice, micromolecular sodium alginate, bletilla striata extracting solution, cell-penetrating peptide, phytosterol and denatured ethyl acetate is 1: 3: 10: 12: 8: 5: 4: 8; the bletilla striata extract is obtained by extracting dried bletilla striata with 20 times of water and performing enzymolysis.

In the step (1), the preparation method of the small-molecule sodium alginate comprises the following steps: adding 1 part by weight of macromolecular sodium alginate into 80 parts by weight of deionized water at 75 ℃, stirring until the macromolecular sodium alginate is completely dissolved, then adding 0.4 part by weight of hydrogen peroxide, stirring and reacting at 70 ℃ for 20 hours, and precipitating with ethanol to obtain the micromolecular sodium alginate; the weight average molecular weight of the macromolecular sodium alginate is 500 kDa. The specific method of alcohol precipitation is to add absolute ethyl alcohol with the volume twice of the reaction solution, precipitate and centrifuge.

In the step (1), the bletilla striata extracting solution is prepared by the following steps of crushing dry bletilla striata to prepare bletilla striata powder, transferring the bletilla striata powder into a differential pressure extractor, condensing water to a temperature of 2 ℃, vacuumizing the differential pressure extractor to-50 MPa within 50 seconds, keeping the pressure for 5 minutes, spraying deionized water at a temperature of 15 ℃ for extraction, introducing purified compressed air, increasing the pressure in the differential pressure extractor to 0MPa within 1 minute, continuing introducing the purified compressed air to enable the pressure in the differential pressure extractor to reach 3MPa, keeping the pressure for 5 minutes, stopping pressurizing, vacuumizing again to enable the pressure in the differential pressure extractor to be reduced to 0MPa, repeating the process from vacuumizing to pressure reduction for a plurality of times, then carrying out solid-liquid separation through centrifugation, filtering centrifugal liquid to obtain filtrate, adding cellulase and β -glucanase into the filtrate, carrying out enzymolysis for 50 minutes at a temperature of 45 ℃ and a pH value of 5, and carrying out enzyme deactivation, so that the bletilla striata extracting solution is obtained, wherein the dosage of the cellulase and the β -glucanase is 0.5 per thousand of.

In the step (1), the preparation method of the fresh bitter gourd juice comprises the following steps: cleaning fresh fructus Momordicae Charantiae, removing pulp, cutting, and squeezing to obtain fresh fructus Momordicae Charantiae juice.

In the step (1), the preparation method of the small molecule chondroitin sulfate comprises the following steps: adding 1 part of high molecular weight shark cartilage chondroitin sulfate into 10 parts of deionized water by weight, stirring for dissolving, then adding 0.05 part of hydrogen peroxide, stirring for reacting for 5 hours at 50 ℃, filtering to obtain filtrate, adjusting the pH value to 5, adding absolute ethyl alcohol to ensure that the final volume concentration is 70%, separating out crystals, centrifuging, and washing with absolute ethyl alcohol to obtain the small molecular chondroitin sulfate.

In the step (1), the preparation method of the micromolecule silk fibroin is as follows: according to parts by weight, 1 part of mulberry silk is subjected to degumming and hydrolysis treatment to obtain a silk fibroin solution, then 0.003 part of elastase is added to react for 5 hours at 30 ℃, enzyme is inactivated, and alcohol precipitation is carried out to obtain the micromolecular silk fibroin. The degumming method comprises the following specific steps: soaking mulberry silk in 6 times of 0.05% sodium carbonate aqueous solution by weight, boiling for 40 min while stirring, repeating for 3 times, taking out, and washing with deionized water for 3 times. The specific method of hydrolysis is as follows: and soaking the degummed mulberry silk in 3 times of potassium chloride solution with the mass concentration of 1.2% by weight, and treating for 2 hours at the temperature of 130 ℃ and under the pressure of 0.6 MPa. The specific method for alcohol precipitation comprises the following steps: adding absolute ethyl alcohol with the volume twice that of the reaction solution, precipitating and centrifuging.

In the step (1), the specific method of modification treatment is as follows: adding the cell-penetrating peptide into the mixed solution, adding SPDP (3- (2-pyridinedimercapto) propionic acid N-hydroxysuccinimide ester) dissolved in dimethyl sulfoxide, and stirring and reacting for 15 hours under the protection of argon. The mass-volume ratio of the cell-penetrating peptide to the mixed solution to the SPDP is 1 g: 60mL of: 6mL of: 0.01 g.

In the step (2), the preparation method of the denatured ethyl acetate is as follows: adding 0.08 part of butanediol, 0.1 part of L-malic acid and 0.02 part of disodium glycyrrhizinate into 1 part of ethyl acetate by weight part, and carrying out ultrasonic oscillation for 30 minutes to obtain the modified ethyl acetate.

In the step (3), the dropping time of the first phase was 70 minutes.

In the step (4), the specific method of electrostatic spinning is as follows: the wire outlet is a No. 6 needle head, the voltage is 15kV, the distance from the spray head to the receiver is 25cm, the spray flow is 0.5ml/h, and the relative humidity is lower than 40%.

In the step (4), ultraviolet disinfection is adopted.

Example 2:

The mass ratio of micromolecular chondroitin sulfate, micromolecular silk fibroin, fresh bitter gourd juice, micromolecular sodium alginate, bletilla striata extracting solution, cell-penetrating peptide, phytosterol and denatured ethyl acetate is 1: 5: 8: 15: 6: 8: 2: 10; the bletilla striata extracting solution is obtained by extracting dried bletilla striata with 15 times of water and performing enzymolysis.

In the step (1), the preparation method of the small-molecule sodium alginate comprises the following steps: adding 1 part by weight of macromolecular sodium alginate into 70 parts by weight of deionized water at 80 ℃, stirring until the macromolecular sodium alginate is completely dissolved, then adding 0.6 part by weight of hydrogen peroxide, stirring and reacting at 60 ℃ for 25 hours, and precipitating with ethanol to obtain the micromolecular sodium alginate; the weight average molecular weight of the macromolecular sodium alginate is 300 kDa. The specific method of alcohol precipitation is to add absolute ethyl alcohol with the volume twice of the reaction solution, precipitate and centrifuge.

In the step (1), the bletilla striata extracting solution is prepared by the following steps of crushing dry bletilla striata to prepare bletilla striata powder, transferring the bletilla striata powder to a pressure difference extractor, cooling the pressure difference extractor to 7 ℃, vacuumizing the pressure difference extractor to-100 MPa within 20 seconds, keeping the pressure difference extractor for 2 minutes, spraying deionized water at 15 ℃ for extraction, introducing purified compressed air, increasing the pressure in the pressure difference extractor to 0MPa within 3 minutes, continuing introducing the purified compressed air to enable the pressure in the pressure difference extractor to reach 3MPa, keeping the pressure for 2 minutes, stopping pressurizing, vacuumizing again to enable the pressure in the pressure difference extractor to be reduced to 0MPa, repeating the process from vacuumizing to pressure reduction for a plurality of times, then carrying out solid-liquid separation through centrifugation, filtering centrifugal liquid to obtain filtrate, adding cellulase and β -glucanase into the filtrate, carrying out enzymolysis for 70 minutes under the conditions of 50 ℃ and pH 4.5, and carrying out enzyme deactivation, so that the bletilla striata extracting solution is obtained, wherein the dosages of the cellulase and β -glucanase are respectively 0.3 per thousand of dry mass.

In the step (1), the preparation method of the small molecule chondroitin sulfate comprises the following steps: adding 1 part of high molecular weight shark cartilage chondroitin sulfate into 12 parts of deionized water by weight, stirring for dissolving, then adding 0.03 part of hydrogen peroxide, stirring for reacting for 3 hours at 55 ℃, filtering to obtain filtrate, adjusting the pH value to 6, adding absolute ethyl alcohol to ensure that the final volume concentration is 60%, separating out crystals, centrifuging, and washing with absolute ethyl alcohol to obtain the small molecular chondroitin sulfate.

In the step (1), the preparation method of the micromolecule silk fibroin is as follows: according to parts by weight, 1 part of mulberry silk is subjected to degumming and hydrolysis treatment to obtain a silk fibroin solution, then 0.005 part of elastase is added, the reaction is carried out for 8 hours at 25 ℃, the enzyme is inactivated, and the ethanol precipitation is carried out to obtain the micromolecule silk fibroin. The degumming method comprises the following specific steps: soaking mulberry silk in 4 times of 0.1% sodium carbonate aqueous solution by weight, boiling for 30 min while stirring, repeating for 4 times, taking out, and washing with deionized water for 2 times. The specific method of hydrolysis is as follows: and soaking the degummed mulberry silk in 5 times of potassium chloride solution with the mass concentration of 1% by weight, and treating for 3 hours at the temperature of 140 ℃ and under the pressure of 0.5 MPa. The specific method for alcohol precipitation comprises the following steps: adding absolute ethyl alcohol with the volume twice that of the reaction solution, precipitating and centrifuging.

In the step (1), the specific method of modification treatment is as follows: adding the cell-penetrating peptide into the mixed solution, adding SPDP (3- (2-pyridinedimercapto) propionic acid N-hydroxysuccinimide ester) dissolved in dimethyl sulfoxide, and stirring and reacting for 10 hours under the protection of argon. The mass-volume ratio of the cell-penetrating peptide to the mixed solution to the SPDP is 1 g: 85 mL: 5mL of: 0.03 g.

In the step (2), the preparation method of the denatured ethyl acetate is as follows: adding 0.06 part of butanediol, 0.2 part of L-malic acid and 0.01 part of disodium glycyrrhizinate into 1 part of ethyl acetate by weight part, and carrying out ultrasonic oscillation for 50 minutes to obtain the modified ethyl acetate.

In the step (3), the dropping time of the first phase was 50 minutes.

In the step (4), the specific method of electrostatic spinning is as follows: the wire outlet is a No. 6 needle head, the voltage is 25kV, the distance from the spray head to the receiver is 20cm, the spray flow is 0.8ml/h, and the relative humidity is lower than 40%.

In the step (4), ultraviolet disinfection is adopted.

Example 3:

The mass ratio of micromolecular chondroitin sulfate, micromolecular silk fibroin, fresh bitter gourd juice, micromolecular sodium alginate, bletilla striata extracting solution, cell-penetrating peptide, phytosterol and denatured ethyl acetate is 1: 4: 9: 13: 7: 6: 3: 9; the bletilla striata extracting solution is obtained by extracting dried bletilla striata with 18 times of water and performing enzymolysis.

In the step (1), the preparation method of the small-molecule sodium alginate comprises the following steps: adding 1 part by weight of macromolecular sodium alginate into 75 parts by weight of deionized water at 78 ℃, stirring until the macromolecular sodium alginate is completely dissolved, then adding 0.5 part by weight of hydrogen peroxide, stirring and reacting at 65 ℃ for 22 hours, and precipitating with ethanol to obtain the micromolecular sodium alginate; the weight average molecular weight of the macromolecular sodium alginate is 400 kDa. The specific method of alcohol precipitation is to add absolute ethyl alcohol with the volume twice of the reaction solution, precipitate and centrifuge.

In the step (1), the bletilla striata extracting solution is prepared by the following steps of crushing dry bletilla striata to prepare bletilla striata powder, transferring the bletilla striata powder to a pressure difference extractor, condensing water to 5 ℃, vacuumizing the pressure difference extractor to-80 MPa within 30 seconds, keeping the pressure difference extractor for 4 minutes, spraying deionized water at 15 ℃ for extraction, introducing purified compressed air, increasing the pressure in the pressure difference extractor to 0MPa within 2 minutes, continuing introducing the purified compressed air to enable the pressure in the pressure difference extractor to reach 3MPa, keeping the pressure for 3 minutes, stopping pressurizing, vacuumizing again to enable the pressure in the pressure difference extractor to be reduced to 0MPa, repeating the process from vacuumizing to pressure reduction for a plurality of times, then carrying out solid-liquid separation through centrifugation, filtering centrifugal liquid to obtain filtrate, adding cellulase and β -glucanase into the filtrate, carrying out enzymolysis for 60 minutes under the conditions of 45-50 ℃ and pH being 4.5, and carrying out enzyme deactivation to obtain the bletilla striata extracting solution, wherein the dosages of the cellulase and the β -glucanase are respectively 0.4 per thousand of the dry bletilla striata mass.

In the step (1), the preparation method of the small molecule chondroitin sulfate comprises the following steps: adding 1 part of high molecular weight shark cartilage chondroitin sulfate into 11 parts of deionized water by weight, stirring for dissolving, then adding 0.04 part of hydrogen peroxide, stirring for reacting for 4 hours at 52 ℃, filtering to obtain filtrate, adjusting the pH value to 6, adding absolute ethyl alcohol to ensure that the final volume concentration is 65%, separating out crystals, centrifuging, and washing with absolute ethyl alcohol to obtain the small molecular chondroitin sulfate.

In the step (1), the preparation method of the micromolecule silk fibroin is as follows: according to parts by weight, 1 part of mulberry silk is subjected to degumming and hydrolysis treatment to obtain a silk fibroin solution, then 0.004 part of elastase is added to react for 6 hours at 28 ℃, enzyme is inactivated, and alcohol precipitation is carried out to obtain the micromolecular silk fibroin. The degumming method comprises the following specific steps: soaking mulberry silk in 5 times of 0.08% sodium carbonate aqueous solution by weight, boiling for 35 minutes while stirring, repeating for 3 times, taking out, and washing with deionized water for 2 times. The specific method of hydrolysis is as follows: and soaking the degummed mulberry silk in 4 times of potassium chloride solution with the mass concentration of 1.1% by weight, and treating for 2 hours at 135 ℃ and 0.5 MPa. The specific method for alcohol precipitation comprises the following steps: adding absolute ethyl alcohol with the volume twice that of the reaction solution, precipitating and centrifuging.

In the step (1), the specific method of modification treatment is as follows: adding the cell-penetrating peptide into the mixed solution, adding SPDP (3- (2-pyridinedimercapto) propionic acid N-hydroxysuccinimide ester) dissolved in dimethyl sulfoxide, and stirring and reacting for 12 hours under the protection of argon. The mass-volume ratio of the cell-penetrating peptide to the mixed solution to the SPDP is 1 g: 75mL of: 5.5 mL: 0.02 g.

In the step (2), the preparation method of the denatured ethyl acetate is as follows: adding 0.07 part of butanediol, 0.15 part of L-malic acid and 0.015 part of disodium glycyrrhizinate into 1 part of ethyl acetate by weight part, and carrying out ultrasonic oscillation for 40 minutes to obtain the modified ethyl acetate.

In the step (3), the dropping time of the first phase was 60 minutes.

In the step (4), the specific method of electrostatic spinning is as follows: the wire outlet is a No. 6 needle head, the voltage is 20kV, the distance from the spray head to the receiver is 22cm, the spray flow is 0.6ml/h, and the relative humidity is lower than 40%.

In the step (4), ultraviolet disinfection is adopted.

Comparative example 1

The procedure of example 1 was repeated except for omitting the small sodium alginate.

Comparative example 2

The same procedure as in example 1 was repeated except that the bletilla striata extract was omitted.

Comparative example 3

The fresh balsam pear juice is omitted and the rest is the same as the example 1.

Comparative example 4

The small molecule chondroitin sulfate was omitted and the rest was the same as in example 1.

Comparative example 5

The procedure of example 1 was repeated except that the small-molecule silk fibroin was omitted.

Comparative example 6

The membrane-penetrating peptide was omitted, and the procedure of example 1 was otherwise repeated.

Comparative example 7

The phytosterols were omitted and the procedure of example 1 was followed.

Comparative example 8

The denatured ethyl acetate was replaced with ethyl acetate, and the rest was the same as in example 1.

Test examples

1. The facial masks obtained in examples 1 to 3 were tested for air permeability (pressure of 100Pa) with reference to GB/T5453-2007, and the mechanical properties of the facial masks obtained in examples 1 to 3 and comparative examples 1, 4 and 5 were tested, and the results are shown in Table 1.

TABLE 1 air permeability and mechanical Properties testing

	Air permeability (mm/s)	Breaking strength (cN/dtex)	Elongation at Break (%)
				Example 1	5896	3.8	12.3
Example 2	5893	3.9	12.3
				Example 3	5905	4.2	12.5
Comparative example 1	--	2.1	7.9
				Comparative example 4	--	1.9	8.2
Comparative example 5	--	2.0	7.8

As can be seen from Table 1, the masks obtained in examples 1 to 3 had good air permeability and good mechanical properties. The small-molecule sodium alginate, the small-molecule chondroitin sulfate and the small-molecule silk fibroin are omitted in the comparative example 1, the small-molecule chondroitin sulfate and the comparative example 4 and the small-molecule silk fibroin in the comparative example 5, so that the hydrogen bonding effect among the small-molecule sodium alginate, the small-molecule chondroitin sulfate and the small-molecule silk fibroin is weakened, the mechanical property of the mask is obviously deteriorated, and the hydrogen bonding effect of the small-molecule sodium alginate, the small-molecule chondroitin sulfate and the small.

2. Examination of whitening and freckle-removing effects

220 women with age of 25-40 years and different degrees of pigments such as chloasma, butterfly spot and the like on the face are selected and randomly divided into 11 groups, and the facial masks of examples 1-3 and comparative examples 1-8 are respectively adopted for carrying out whitening and freckle removing investigation. The results are shown in Table 2.

The application method of the mask comprises the following steps: the facial mask is used every night, the facial mask is taken out from a packaging bag and then directly pasted on the face, a layer of warm water with the temperature of 35-40 ℃ is uniformly sprayed on the surface of the facial mask by using a skin-care spraying pot, the facial mask gradually melts (the dissolving time is less than 10 minutes) and is absorbed by the skin, and the facial mask does not need to be cleaned after being used. The application period is 6 months.

And (3) judging standard: the basic cure is as follows: the visual stain of naked eyes basically disappears; the effect is shown: the visual stain fades by more than 60 percent; the method has the following advantages: the visual stain fades by more than 20 percent; and (4) invalidation: the visual stain faded to less than 20%.

The skin whiteness of the subjects before and after use (15 points were arbitrarily selected for the face skin, and the average value was obtained) was measured using a colorimeter, and the whiteness increase rate (after use whiteness-before use whiteness)/before use whiteness was calculated.

TABLE 2 comparison of whitening and speckle removing effects

As can be seen from table 2, the facial masks obtained in examples 1 to 3 had good whitening and spot-removing effects. The small-molecular sodium alginate is omitted in comparative example 1, the bletilla striata extracting solution is omitted in comparative example 2, the small-molecular sodium alginate is added into fresh bitter gourd juice, calcium is contained in the fresh bitter gourd juice to form calcium alginate, the uniform dispersion of products is not facilitated, the fresh bitter gourd juice is omitted in comparative example 3, the small-molecular chondroitin sulfate is omitted in comparative example 4, the small-molecular silk fibroin is omitted in comparative example 5, the membrane-penetrating peptide is omitted in comparative example 6, the phytosterol is omitted in comparative example 7, the denatured ethyl acetate is replaced by the ethyl acetate in comparative example 8, the whitening and freckle-removing effects are obviously poor, and the whitening and freckle-removing effects.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A preparation method of a dissolving mask for whitening and removing freckles is characterized by comprising the following specific steps:

2. The preparation method of claim 1, wherein the mass ratio of the small-molecule chondroitin sulfate, the small-molecule silk fibroin, the fresh bitter gourd juice, the small-molecule sodium alginate, the bletilla striata extracting solution, the cell-penetrating peptide, the phytosterol and the denatured ethyl acetate is 1: 3-5: 8-10: 12-15: 6-8: 5-8: 2-4: 8-10; the bletilla striata extracting solution is obtained by extracting dried bletilla striata with water 15-20 times of the weight of the dried bletilla striata and performing enzymolysis.

3. The preparation method of claim 1, wherein in the step (1), the preparation method of the small-molecule sodium alginate comprises the following steps: adding 1 part by weight of macromolecular sodium alginate into 70-80 parts by weight of deionized water at 75-80 ℃, stirring until the macromolecular sodium alginate is completely dissolved, then adding 0.4-0.6 part by weight of hydrogen peroxide, stirring and reacting at 60-70 ℃ for 20-25 hours, and carrying out alcohol precipitation to obtain the micromolecular sodium alginate; the weight average molecular weight of the macromolecular sodium alginate is 300-500 kDa.

4. The method according to claim 1, wherein the small chondroitin sulfate is prepared in the step (1) as follows: adding 1 part by weight of high molecular weight shark cartilage chondroitin sulfate into 10-12 parts by weight of deionized water, stirring for dissolving, then adding 0.03-0.05 part by weight of hydrogen peroxide, stirring for reacting at 50-55 ℃ for 3-5 hours, filtering to obtain filtrate, adjusting the pH to 5-6, adding absolute ethyl alcohol to make the volume final concentration of the absolute ethyl alcohol to be 60-70%, precipitating crystals, centrifuging, and washing with absolute ethyl alcohol to obtain the micromolecular chondroitin sulfate.

5. The method according to claim 1, wherein in step (1), the small-molecule silk fibroin is prepared by the following method: according to parts by weight, 1 part of mulberry silk is subjected to degumming and hydrolysis treatment to obtain a silk fibroin solution, then 0.003-0.005 part of elastase is added, the reaction is carried out for 5-8 hours at 25-30 ℃, enzyme deactivation and alcohol precipitation are carried out, and the micromolecule silk fibroin is obtained.

6. The method according to claim 1, wherein in the step (1), the modification treatment is performed by: adding the cell-penetrating peptide into the mixed solution, then adding SPDP (3- (2-pyridinedimercapto) propionic acid N-hydroxysuccinimide ester) dissolved in dimethyl sulfoxide, and stirring and reacting for 10-15 hours under the protection of argon.

7. The method according to claim 1, wherein in the step (2), the denatured ethyl acetate is prepared as follows: adding 0.06-0.08 part of butanediol, 0.1-0.2 part of L-malic acid and 0.01-0.02 part of disodium glycyrrhizinate into 1 part of ethyl acetate by weight part, and carrying out ultrasonic oscillation for 30-50 minutes to obtain the modified ethyl acetate.

8. The method according to claim 1, wherein the dropping time of the first phase in the step (3) is 50 to 70 minutes.

9. The preparation method according to claim 1, wherein in the step (4), the specific method of electrospinning is: the wire outlet is a No. 6 needle head, the voltage is 15-25 kV, the distance from the spray head to the receiver is 20-25 cm, the spray flow is 0.5-0.8 ml/h, and the relative humidity is lower than 40%.

10. A whitening and freckle-removing dissolving mask obtained by the preparation method of any one of claims 1-9.