CN110869501A - 一种高效的内切酶缓冲液体系 - Google Patents

一种高效的内切酶缓冲液体系 Download PDF

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Publication number
CN110869501A
CN110869501A CN201780090482.6A CN201780090482A CN110869501A CN 110869501 A CN110869501 A CN 110869501A CN 201780090482 A CN201780090482 A CN 201780090482A CN 110869501 A CN110869501 A CN 110869501A
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mass
endo
endonuclease buffer
buffer solution
hcl
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CN110869501B (zh
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张长领
刘二凯
陈奥
章文蔚
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MGI Tech Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
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  • Genetics & Genomics (AREA)
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  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

一种能提高Endo IV酶活性的内切酶缓冲液，包括20~200mM的Tris‑HCl，0.05~0.2M的NaCl，2~20mM的MgSO4，0.2~10mM的DTT，0.05~5质量%的表面活性剂，pH为7~8。所述缓冲液还可进一步包括0.1~5质量%的碳原子数小于5的短链醇类。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201780090482.6A 2017-08-01 2017-08-01 一种高效的内切酶缓冲液体系 Active CN110869501B (zh)

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PCT/CN2017/095470 WO2019023937A1 (zh) 2017-08-01 2017-08-01 一种高效的内切酶缓冲液体系

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CN110869501A true CN110869501A (zh) 2020-03-06
CN110869501B CN110869501B (zh) 2023-08-18

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050191682A1 (en) * 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
CN101040041A (zh) * 2004-09-17 2007-09-19 申特莱恩公司 质粒dna的稳定液体制剂
CN101974638A (zh) * 2010-11-10 2011-02-16 华东医学生物技术研究所 连接反应偶联核酸侵入反应与切刻内切酶反应的核酸信号扩增检测方法
CN115029333A (zh) * 2021-03-05 2022-09-09 武汉核圣生物技术有限公司 一种核酸内切酶及其纯化方法和应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017173A1 (en) * 2003-07-29 2005-02-24 Sigma Aldrich Co. Methods and compositions for amplification of dna
JP4836952B2 (ja) * 2004-07-30 2011-12-14 ジュノプラント−ヴァロール 高感受性エンドヌクレアーゼを生産する方法、新規なエンドヌクレアーゼ調製物、及びそれらの使用
CN103602734A (zh) * 2013-11-04 2014-02-26 北京海思特临床检验所有限公司 用于检测egfr基因热点突变的引物、试剂盒和检测方法
CN105506078B (zh) * 2015-12-18 2019-07-09 山东大学 一种用于平行测定尿嘧啶-dna糖基化酶和内切酶iv活性的方法及其应用和试剂盒

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050191682A1 (en) * 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
CN101040041A (zh) * 2004-09-17 2007-09-19 申特莱恩公司 质粒dna的稳定液体制剂
CN101974638A (zh) * 2010-11-10 2011-02-16 华东医学生物技术研究所 连接反应偶联核酸侵入反应与切刻内切酶反应的核酸信号扩增检测方法
CN115029333A (zh) * 2021-03-05 2022-09-09 武汉核圣生物技术有限公司 一种核酸内切酶及其纯化方法和应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ROBERT J. FORSTER 等: "Nucleic acid extraction, oligonucleotide probes and PCR methods" *
YUKI YOSHINO等: "A new 10-min ligation method using a modified buffer system with a very low amount of T4 DNA ligase: the ‘‘Coffee Break Ligation’’ technique" *

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Publication number Publication date
CN110869501B (zh) 2023-08-18
WO2019023937A1 (zh) 2019-02-07

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