CN110850039A - Dynamic swing type intestinal tract in-vitro substitution experimental device - Google Patents
Dynamic swing type intestinal tract in-vitro substitution experimental device Download PDFInfo
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- CN110850039A CN110850039A CN201911259466.2A CN201911259466A CN110850039A CN 110850039 A CN110850039 A CN 110850039A CN 201911259466 A CN201911259466 A CN 201911259466A CN 110850039 A CN110850039 A CN 110850039A
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- 210000001035 gastrointestinal tract Anatomy 0.000 title claims abstract description 71
- 238000006467 substitution reaction Methods 0.000 title claims abstract description 46
- 238000000338 in vitro Methods 0.000 title claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 125
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 65
- 238000002474 experimental method Methods 0.000 claims abstract description 58
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 32
- 235000015097 nutrients Nutrition 0.000 claims abstract description 30
- 239000002253 acid Substances 0.000 claims abstract description 28
- 239000000243 solution Substances 0.000 claims abstract description 28
- 230000007246 mechanism Effects 0.000 claims abstract description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000001301 oxygen Substances 0.000 claims abstract description 19
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 19
- 238000011160 research Methods 0.000 claims abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 9
- 230000000968 intestinal effect Effects 0.000 claims description 134
- 239000007788 liquid Substances 0.000 claims description 36
- 238000007789 sealing Methods 0.000 claims description 35
- 239000003513 alkali Substances 0.000 claims description 26
- 239000007789 gas Substances 0.000 claims description 17
- 239000002585 base Substances 0.000 claims description 11
- 230000001105 regulatory effect Effects 0.000 claims description 11
- 241001465754 Metazoa Species 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 8
- 210000002429 large intestine Anatomy 0.000 claims description 8
- 238000004088 simulation Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 238000005260 corrosion Methods 0.000 claims description 5
- 230000007797 corrosion Effects 0.000 claims description 5
- 230000001681 protective effect Effects 0.000 claims description 5
- 238000003556 assay Methods 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 4
- 230000008855 peristalsis Effects 0.000 claims description 4
- 238000011161 development Methods 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims description 3
- 239000003779 heat-resistant material Substances 0.000 claims description 2
- 239000011810 insulating material Substances 0.000 claims description 2
- 230000001788 irregular Effects 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 claims 1
- 230000018109 developmental process Effects 0.000 claims 1
- 229910001873 dinitrogen Inorganic materials 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 239000012670 alkaline solution Substances 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000000452 restraining effect Effects 0.000 description 4
- 230000001079 digestive effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000791420 Plica Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000008558 metabolic pathway by substance Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
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- Chemical & Material Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention provides a dynamic swing type intestinal tract in-vitro substitution experimental device, which comprises a swing mechanism, a temperature control water jacket, an intestinal tract assembly, a nitrogen pressure bottle, an alkaline solution bottle, an acid solution bottle, a nutrient solution bottle, a collecting bottle and a main control computer, wherein the swing mechanism drives the temperature control water jacket and the intestinal tract assembly to swing under the driving of a swing motor to finish the intestinal tract wriggling function, the intestinal tract assembly is wrapped in the temperature control water jacket and keeps the temperature under the heating of a water bath pump and an electric control warmer, reagents in the intestinal tract assembly and an experimental sample are sealed and isolated to form a specific anaerobic pH environment, a temperature sensor, an acid-base sensor, a pressure sensor and an oxygen sensor of the intestinal tract assembly control a nitrogen inlet, an alkaline solution inlet, an acid solution inlet and a metering pump, a collecting valve, a water bath pump, an electric control warmer and a, the invention has the beneficial effects that: the device can simulate the living intestinal tract in vitro to carry out dynamic intestinal tract in vitro substitution experiments, and meets the requirements of standardized scientific research and application.
Description
Technical Field
The invention relates to a dynamic swing type intestinal tract in-vitro substitution experimental device.
Background
As the researches of digestive absorption in natural science researches such as food nutrition, functional active substance metabolism research, drug release power research, probiotics and prebiotics, food toxicology research, animal nutrition and feed research and the like all need to carry out digestive absorption experiments for predicting or evaluating the digestibility, bioavailability, release kinetic characteristics, structural change and the like of compounds, in the digestive absorption experiments of the prior scientific researches, human or animal living bodies are often adopted for experiments or simple intestinal tract simulation devices are used for replacing experiments, as the living body experiments have great individual differences, and the intestinal tract environment of the living bodies in the experimental process can not completely ensure the normal environment, the simple intestinal tract simulation devices can completely simulate the shape and the internal environment conditions of the human or animal large intestine but can not simulate the dynamic action of the human or animal large intestine, therefore, the experimental sample for scientific research taken out after the experiment can not correctly reflect the digestion and absorption conditions of the intestinal tract, the repeatability and the accuracy of the experiment can not be ensured, the conclusion of the scientific research can be influenced to a certain extent, the requirement of the current scientific research can not be met, and the development of related scientific research is restricted. Therefore, the development of a dynamic swing type intestinal tract in-vitro substitution experimental device for simulating the living intestinal tract in vitro to perform dynamic intestinal tract in-vitro substitution experiments has become an urgent need at present.
Disclosure of Invention
In order to solve the above problems, the present invention provides a dynamic swing type intestinal tract in vitro substitution experiment device, which adopts a method of intelligently setting the swing frequency and amplitude to swing and simulate the living intestinal tract peristalsis, and performs various dynamic intestinal tract in vitro substitution experiments under simulated human or animal large intestine shape and internal environment conditions.
In order to achieve the above purpose, the technical scheme of the invention is to provide a dynamic swing type intestinal tract in-vitro substitution experimental device, which comprises a supporting rack with an elastic foot pad, wherein a nitrogen pressure bottle, an alkaline solution bottle, an acid solution bottle, a nutrient solution bottle and a collecting bottle are arranged on the upper surface of a rigid bedplate at the bottommost part of the supporting rack, a main control computer is also arranged on the upper surface of the rigid bedplate, a computer hardware system and a special software program system carry out data arrangement, parameter analysis and storage recording and control experiment execution through various parameters of an experimental sample of an intestinal tract assembly received by a control lead of an execution system, a restraining guard shaft arranged on a rear wall plate of the supporting rack is matched with a supporting rotating shaft to support a swing mechanism and swing left and right under the driving of a swing motor arranged below so as to drive a temperature control water jacket arranged on the swing mechanism and an intestinal tract assembly in the temperature control water jacket to swing left and right to simulate, the main control computer sets the swing frequency and amplitude in advance to carry out various dynamic intestinal tract in-vitro substitution experiments, a movable wall plate of the swing mechanism is fixedly provided with a temperature control water jacket with a water bath pump and an electric control heater, the temperature control water jacket is wrapped with an intestinal tract assembly, the temperature control water jacket heats and controls the temperature of the intestinal tract assembly at any time to ensure that the experimental simulation temperature in an intestinal tract inner cavity of the intestinal tract assembly is always in centigrade or other stable temperature required to meet the requirements of the intestinal tract in-vitro substitution experiments, the bottom end of the intestinal tract assembly is provided with a collection outlet which is connected into a collection bottle through a collection liquid pipe and a collection valve, the upper opening of the intestinal tract cavity at the uppermost end of the intestinal tract assembly is provided with a large sealing cover for sealing and isolating the inner environment and the outer environment, the large sealing cover is provided with an air vent with a pressure regulating air valve for discharging oxygen to form an anaerobic environment in the intestinal tract assembly after nitrogen is filled, and the sample port of the The intestinal tract assembly simulates human or animal large intestine and comprises a dome-shaped intestinal wall consisting of a plurality of annular bags and annular plica, the inside of the intestinal tract assembly is an intestinal tract inner cavity for accommodating various reagents and experimental samples to simulate an in-vitro substitution experiment in the intestinal tract, water jacket temperature sensors, acid-base sensors, pressure sensors and oxygen sensors are sequentially arranged on two sides of the annular bags on the dome-shaped intestinal wall and are connected to a main control computer through control wires to transmit experimental data information in a temperature control water jacket and the intestinal tract assembly, and the main control computer controls a nitrogen inlet, an alkali liquor inlet, an acid liquor inlet, a nutrient solution inlet and respective gas metering pumps, liquid metering pumps, collecting valves, water bath pumps, an electric control warmer and a swing motor of a standby inlet with an inlet cover to maintain an anaerobic environment in the intestinal tract inner cavity of the intestinal tract assembly during the experiment through the control wires, The pH value and the nutrient solution are required to meet various requirements of intestinal tract in-vitro substitution experiments.
The invention has the beneficial effects that: the shape and internal environmental conditions of the living intestinal tract are simulated in vitro, and dynamic intestinal tract in-vitro substitution experiments are carried out, so that standardized scientific researches and applications are met.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention.
In the figure:
1. alkali liquor bottle 2, acid liquor bottle 3, nutrient liquor bottle 4, collection outlet 5, transfusion bent pipe 6, water bath connecting pipe 7 and water bath pump
8. Water jacket lower wall 9, water bath connecting pipe interface 10, liquid metering pump 11, alkali liquor inlet 12, water bath inner chamber 13 and acid liquor inlet
14. One-way valve A15, nutrient solution inlet 16, dome-shaped intestinal wall 17, intestinal assembly 18, inlet cover plug 19 and standby inlet
20. Temperature control water jacket 21, restraint chute 22, swing mechanism 23, movable wall plate 24, upper cover rubber ring 25 and upper opening of intestinal cavity
26. Pressure regulating air valve 27, air release opening 28, restraining protective shaft 29, sample adding opening 30, small sealing cover 31 and large sealing cover
32. Water jacket upper wall 33, oxygen sensor 34, water jacket temperature sensor 35, rear wall plate 36, ring-like bag 37, fixing support leg
38. Pressure sensor 39, water jacket side wall 40, acid-base sensor 41, control wire 42, annular wall
43. Temperature sensor 44, intestinal lumen 45, nitrogen inlet 46, one-way valve B47, gas metering pump 48 and water jacket interface
49. Lower intestinal cavity opening 50, gas transmission elbow 51, electric control warmer 52, collection valve 53, driven gear 54, support rotating shaft
55. Semicircular movable plate 56, nitrogen pressure bottle 57, elastic foot pad 58, rigid platen 59, support rack 60 and main control computer
61. The motor bracket 62, the swing motor 63, the driving gear 64, the collecting liquid pipe 65 and the collecting bottle.
Detailed Description
The invention is described with reference to the accompanying drawings and examples.
As shown in the attached drawings, the invention provides a dynamic swing type intestinal tract in-vitro substitution experimental device, which comprises a supporting rack 59 with an elastic foot pad 57, wherein the supporting rack 59 is a table type support made of firm corrosion-resistant rigid materials and capable of supporting a plurality of functional components, the bottommost part of the supporting rack 59 is a planar rigid bedplate 58 with the elastic foot pad 57, a nitrogen pressure bottle 56, an alkali liquid bottle 1, an acid liquid bottle 2, a nutrient liquid bottle 3 and a collecting bottle 65 are arranged on the upper surface of the rigid bedplate 58, a main control computer 60 is also arranged on the upper surface of the rigid bedplate 58, a fixed rear wall plate 35 is vertically arranged at the rear part of the rigid bedplate 58, a restraining protective shaft 28 arranged on the rear wall plate 35 is matched with a supporting rotating shaft 54 to support a swing mechanism 22 and driven by a swing motor 62 arranged on the lower side to swing left and right so as to drive a peristaltic mechanism 20 arranged on the swing mechanism 22 and an intestinal tract assembly 17 in a temperature control water jacket 20 to swing left and right The main control computer 60 sets the swing frequency and amplitude in advance to carry out various dynamic intestinal tract in-vitro substitution experiments, the intestinal tract assembly 17 is an irregular thin-wall container which is made of firm acid and alkali corrosion resistant materials and simulates the shape of a human or animal large intestine, the temperature of the container is controlled by the wrapping of the temperature control water jacket 20, the bottom end of a funnel-shaped intestinal cavity lower opening 49 at the bottom end of the intestinal tract assembly 17 is provided with a collection outlet 4 which is connected into a collection bottle 65 arranged on a rigid bedplate 58 of a support bench 59 through a collection liquid pipe 64 and a collection valve 52, the uppermost end of the intestinal tract assembly 17 is an intestinal cavity upper opening 25 with an external thread buckle, a large sealing cover 31 with an internal thread buckle is matched to seal and isolate the internal and external environments, the large sealing cover 31 is a circular sealing cover made of the acid and alkali corrosion resistant materials, the inner wall of the opening cover is provided with an upper cover rubber ring 24 and an internal thread buckle matched with the, after being screwed, the internal and external environments of the intestinal canal inner cavity 44 of the intestinal canal assembly 17 are sealed and isolated, the upper surface of the large sealing cover 31 is provided with two round holes with different inner diameters and sealing rubber rings, the round hole with the small inner diameter is provided with an air vent 27 with a pressure regulating air valve 26, after the intestinal assembly 17 is filled with nitrogen, oxygen is discharged under the control of the pressure regulating air valve 26 so as to form an anaerobic environment in an intestinal cavity 44 of the intestinal assembly 17, a sample adding port 29 with a small sealing cover 30 is arranged in a round hole with a large inner diameter, various reagents and fluid or liquid samples required by the experiment are injected into the intestinal cavity 44 of the intestinal assembly 17 at any time for the experiment, the fluid or liquid samples can be extracted from the intestinal cavity 44 of the intestinal assembly 17 at any time for assay analysis, and the positions of the air releasing port 27 on the large sealing cover 31 and the sample adding port 29 of the small sealing cover 30 can be adjusted up and down so as to adapt to different sample volumes and property requirements during the experiment; a plurality of ring-shaped continuous raised ring-like bags 36 and dome-shaped intestinal walls 16 of ring-like folds 42 at the connection part are seamlessly connected between an intestinal cavity lower opening 49 and an intestinal cavity upper opening 25 to form a closed intestinal cavity 44, in-vitro substitution experiments for simulating intestinal tracts are carried out by containing various reagents and experimental samples, a nitrogen inlet 45, an alkali liquor inlet 11, an acid liquor inlet 13, a nutrient solution inlet 15, a standby inlet 19 with an inlet cover 18, a water jacket temperature sensor 34, a temperature sensor 43, an acid-alkali sensor 40, a pressure sensor 38 and an oxygen sensor 33 are sequentially arranged at two sides of the ring-like bags 36 on the dome-shaped intestinal walls 16, the nitrogen inlet 45 is filled with nitrogen from a nitrogen pressure bottle 56 arranged on a rigid bedplate 58 of a supporting bench 59 through a gas transmission elbow 50 under the control of a one-way valve B46 and a gas-like metering pump 47, and a gas outlet 27 arranged on the large air vent 31 is forced to discharge oxygen under the control of a pressure regulating valve 26 so as to form an anaerobic environment in the intestinal cavity 44, the alkali liquor inlet 11, the acid liquor inlet 13 and the nutrient solution inlet 15 are respectively pumped and filled into the intestinal canal inner cavity 44 of the intestinal canal assembly 17 from the alkali liquor bottle 1, the acid liquor bottle 2 and the nutrient solution bottle 3 which are arranged on the rigid bedplate 58 of the supporting bench 59 through respective infusion bent pipes 5 under the control of respective one-way valves A14 and liquid metering pumps 10 so as to adjust the pH value and the nutrient solution requirement to meet the requirements of the intestinal canal extracorporeal substitution experiment, the liquid metering pump 10, the gas metering pump 47, the water jacket temperature sensor 34, the temperature sensor 43, the acid-base sensor 40, the pressure sensor 38, the oxygen sensor 33 and the collecting valve 52 are all connected to the main control computer 60 through control wires 41 to be controlled, the temperature control water jacket 20 is a round pipe-shaped closed container made of firm transparent heat-resistant material, the uppermost edge of the temperature control water jacket 20 is a round sheet-shaped water jacket wall 32 with a round opening at the center, the central round mouth of the upper water jacket wall 32 is in seamless connection with the upper intestinal cavity mouth 25 of the intestinal assembly 17, the excircle of the upper water jacket wall 32 is in seamless connection with the upper end of a round tube-shaped water jacket side wall 39, the lower end of the water jacket side wall 39 is in seamless connection with a circle of the lower water jacket wall 8 in a shape of a circular sheet, the exterior of the bottommost end of the intestinal assembly 17 is fixedly connected with the water jacket lower wall 8 of the temperature control water jacket 20 in a sealing way, an internal closed water bath inner chamber 12 is formed between the water jacket side wall 39 of the temperature control water jacket 20 and the intestinal assembly 17, the intestinal assembly 17 is wrapped in the temperature control water jacket 20, the temperature control water jacket 20 heats and controls the temperature of the intestinal assembly 17 at; the two sides of the upper and lower ends of the water jacket side wall 39 are respectively provided with a plurality of fixed support legs 37 to fix the whole temperature control water jacket 20 and the intestinal assembly 17 therein on the movable wall plate 23 of the swing mechanism 22, the water jacket side wall 39 is fixedly provided with a water jacket temperature sensor 34 which is connected with a main control computer 60 through a control lead 41 to monitor the temperature of temperature control hot water in the water bath inner chamber 12, the two sides of the water jacket lower wall 8 are respectively provided with a water jacket interface 48 which is vertically opened downwards, the water bath connecting pipe interface 9 is communicated with a water bath pump 7 and an electric control warmer 51 which are fixed below a movable wall plate 23 of the swinging mechanism 22 through a water bath connecting pipe 6, and temperature-controlled hot water generated by the electric control warmer 51 is sent into a water bath inner cabin 12 of a temperature-controlled water jacket 20 to heat and control the temperature of the intestinal assembly 17 at any time so as to ensure that the experimental simulation temperature in the intestinal cavity 44 of the intestinal assembly 17 is always 37 ℃ or other stable temperatures with other requirements to meet the requirements of the intestinal in-vitro substitution experiment; the swinging mechanism 22 is a mechanical part which is made of rigid insulating materials and can swing and support the temperature control water jacket 20 and the intestinal assembly 17, the main element of the swinging mechanism 22 is a movable wall plate 23 which is made of an approximately elliptical thin plate, the edge of the upper end of the movable wall plate 23 is provided with a semicircular narrow and long open constraint sliding chute 21, a thin shaft-shaped constraint protective shaft 28 which is vertically arranged at the upper end of a rear wall plate 35 of a support rack 59 penetrates through the constraint sliding chute 21 to play a role of freely constraining the movable wall plate 23 to slide and swing on the support rack 59 from left to right, the lower end of the movable wall plate 23 extends out a small semicircular movable plate 55, the circle center of the semicircular movable plate 55 is provided with a freely rotatable support rotating shaft 54, the support rotating shaft 54 penetrates through the circle center of the semicircular plate 55 of the swinging mechanism 22 to support the movable wall plate 23 on the rear wall plate 35 of the support rack 59 to freely swing within the constraint range of the, a swinging motor 62 with a driving gear 63 and connected to a main control computer 60 through a control wire 41 is fixedly arranged at the middle position of the lower part of the rear wall plate 35 by a motor bracket 61, driven gears 53 are uniformly distributed on the excircle of the semicircular movable plate 55 and are meshed with the driving gear 63 of the swinging motor 62 at the lower side, the temperature control water jacket 20 with the water bath pump 7 and the electric control warmer 51 and the intestinal assembly 17 therein are fixedly fixed at the middle position of the movable wall plate 23 through a plurality of fixed supporting legs 37, when the swinging motor 62 rotates forward and backward under the control of the main control computer 60, the driving gear 63 is driven to drive the semicircular movable plate 55 to rotate forward and backward, and the elliptic movable wall plate 23 is driven by the semicircular movable plate 55 to swing left and right under the restraint of the supporting rotating shaft 54 and the restraint protecting shaft 28 so as to drive the whole temperature control water jacket 20 and the intestinal assembly 17 therein to, the main control computer 60 can set the frequency and amplitude of the swing in advance to carry out various dynamic intestinal tract in-vitro substitution experiments; the main control computer 60 is composed of a computer hardware system special for scientific research, a special software program system special for intestinal tract in vitro substitution experiment and an execution system of each application electric element connected to the intestinal tract assembly 17, the swing mechanism 22 and the support rack 59, the computer hardware system and the special software program system are combined in the main control computer 60, the main control computer 60 is arranged on a rigid bedplate 58 of the support rack 59, and is sequentially connected to the water jacket temperature sensor 34, the temperature sensor 43, the acid-base sensor 40, the pressure sensor 38 and the oxygen sensor 33 through a control lead 41 of the execution system to control the temperature of the temperature control water jacket 20 and the parameters of the experimental samples in the intestinal tract inner cavity 44 of the intestinal tract assembly 17 to perform data arrangement, parameter analysis, storage and recording and timely feedback to the main control computer 60, and sequentially controls and drives the nitrogen inlet 45, the nitrogen inlet 45 and the nitrogen outlet in the dome-shaped intestinal wall 16 of the intestinal tract assembly 17 through the control lead 41, The gas metering pump 47, the liquid metering pump 10, the collection valve 52, the water bath pump 7, the electric control warmer 51 and the swing motor 62 which are respectively arranged on the alkali liquor inlet 11, the acid liquor inlet 13, the nutrient solution inlet 15 and the standby inlet 19 work to realize the whole dynamic intestinal tract in-vitro substitution experiment.
Example (b):
when in use, firstly preparing a test sample for replacing experiments for experiments, emptying the liquid in the collecting bottle 65, filling the alkali liquid, the acid liquid and the nutrient liquid in the alkali liquid bottle 1, the acid liquid bottle 2 and the nutrient liquid bottle 3, checking whether the nitrogen in the nitrogen pressure bottle 56 is sufficient, then electrifying the main control computer 60, setting the swinging frequency and amplitude of the swinging motor 62 in a special software program system, opening the one-way valve B46 and the pressure regulating air valve 26, filling the nitrogen into the intestinal cavity 44 from the nitrogen pressure bottle 56 arranged on the rigid bedplate 58 of the supporting bench 59 through the nitrogen inlet 45 under the control of the one-way valve B46 and the gas type metering pump 47 through the gas transmission elbow 50, forcing the air vent 27 arranged on the large sealing cover 31 to discharge the oxygen under the control of the pressure regulating air valve 26 so as to form an oxygen-free environment in the intestinal cavity 44, opening the alkali liquid inlet 11, and discharging the nitrogen, The acid liquor inlet 13 and the nutrient solution inlet 15 are connected with each one-way valve A14, the alkali liquor inlet 11, the acid liquor inlet 13 and the nutrient solution inlet 15 respectively suck alkali liquor, acid liquor and nutrient solution from the alkali liquor bottle 1, the acid liquor bottle 2 and the nutrient solution bottle 3 which are arranged on the rigid bedplate 58 of the supporting bench 59 through respective infusion bent pipes 5 under the control of the respective one-way valve A14 and the liquid metering pump 10 to fill the intestinal lumen 44 of the intestinal assembly 17 with the adjusted pH value and nutrient solution requirements to meet various requirements of intestinal in-vitro substitution experiments, the small sealing cover 30 on the upper surface of the large sealing cover 31 is opened to fill a test sample required by the accurate experiment from the sample adding port 29 to the intestinal lumen 44 of the intestinal assembly 17, and then the small sealing cover 30 is tightly closed; the special software program system of the main control computer 60 controls the start of the experiment, when the swing motor 62 rotates forward and backward according to the preset swing frequency and amplitude under the control of the main control computer 60, the driving gear 63 drives the semi-circular movable plate 55 to rotate forward and backward, the elliptical movable wall plate 23 is driven by the semi-circular movable plate 55 to swing left and right under the restraint of the supporting rotating shaft 54 and the restraining protecting shaft 28, so as to drive the whole temperature control water jacket 20 and the intestinal assembly 17 therein to swing left and right to simulate the intestinal peristalsis, during the experiment, the temperature of the temperature control water jacket 20 received by the water jacket temperature sensor 34, the temperature sensor 43, the acid-base sensor 40, the pressure sensor 38 and the oxygen sensor 33 and various parameters of the experimental sample in the intestinal lumen 44 of the intestinal assembly 17 are fed back to the main control computer 60 at any time, and the main control computer 60 sequentially controls and drives the nitrogen inlet 45 mounted on the dome-shaped intestinal wall 16 of, The gas metering pump 47, the liquid metering pump 10, the collection valve 52 of the alkali liquor inlet 11, the acid liquor inlet 13, the nutrient solution inlet 15 and the standby inlet 19 respectively, and the water bath pump 7, the electric control warmer 51 and the swing motor 62 which are arranged on the movable wall plate 23 of the swing mechanism 22 and the rear wall plate 35 of the support stand 59 work, when the water jacket temperature sensor 34 detects that the temperature of the temperature-controlled hot water in the temperature-controlled water jacket 20 is not enough, the main control computer 60 starts the water bath pump 7 to work, the temperature-controlled hot water generated by the electric-controlled warmer 51 is sent into the water bath inner chamber 12 of the temperature-controlled water jacket 20 from the water jacket interface 48 of the water jacket lower wall 8 through the water bath connecting pipe 6 to heat and control the temperature of the intestinal assembly 17 at any time so as to ensure that the experimental simulation temperature in the intestinal inner chamber 44 of the intestinal assembly 17 is always 37 ℃ or other stable temperatures with other requirements to meet the requirements of the in vitro substitution experiment of the intestinal tract; when various parameters of an experimental sample in the intestinal lumen 44 of the intestinal assembly 17 received by the main control computer 60 through the temperature sensor 43, the acid-base sensor 40, the pressure sensor 38 and the oxygen sensor 33 do not meet experimental requirements, the gas metering pump 47 and the liquid metering pump 10 are started to work, alkali liquor, acid liquor, nutrient solution and nitrogen are supplemented, and the pH value and the nutrient solution requirements are adjusted to meet various requirements of intestinal in-vitro substitution experiments in an oxygen-free environment; in the experiment process, the small sealing cover 30 on the upper surface of the large sealing cover 31 can be opened at any time, a fluid or liquid sample is extracted from the intestinal cavity 44 of the intestinal assembly 17 through the sample adding port 29 for assay analysis, and then the small sealing cover 30 is tightly closed; the main control computer 60 controls the swing motor 62 to work at a certain swing frequency and amplitude at any time; after the experiment is completed, the collection valve 52 is opened, the reacted sample is collected into the collection bottle 64, then the gas metering pump 47, the liquid metering pump 10, the collection valve 52, the water bath pump 7, the electric control heater 51 and the swing motor 62 are controlled to stop working, the special software program system of the main control computer is quitted, the power supply of the main control computer 60 is closed, and the sample pumped out of the substitution experiment is further tested, observed and analyzed, so that the substitution experiment can be used for predicting or evaluating the digestibility, the bioavailability, the release kinetic characteristics, the structural change and other researches.
Claims (7)
1. The utility model provides an external substitution experimental apparatus of formula intestinal is swayd to developments, characterized by: the device comprises a supporting rack (59) with an elastic foot pad (57), wherein a nitrogen pressure bottle (56), an alkali liquor bottle (1), an acid liquor bottle (2), a nutrient solution bottle (3) and a collecting bottle (65) are arranged on the upper surface of a rigid bedplate (58) at the bottommost part, a main control computer (60) is also arranged on the upper surface of the rigid bedplate (58), a computer hardware system and a special software program system carry out data arrangement, parameter analysis and storage recording and control experiment execution through various parameters of an experimental sample of the intestinal assembly (17) received by a control lead (41) of an execution system, a constraint protective shaft (28) arranged on the upper side of a rear wall plate (35) of the supporting rack (59) is matched with a supporting rotating shaft (54) to support a swinging mechanism (22) and driven by a swinging motor (62) arranged below to swing left and right so as to drive the temperature control water jacket (20) arranged on the swinging mechanism (22) and the intestinal assembly (17) in the temperature control water jacket (20) to left and right The device has the advantages that the device can be swung to simulate the intestinal peristalsis, a main control computer (60) sets the swinging frequency and amplitude in advance to carry out various dynamic intestinal in-vitro substitution experiments, a movable wall plate (23) of a swinging mechanism (22) is fixedly provided with a temperature control water jacket (20) with a water bath pump (7) and an electric control warmer (51), the temperature control water jacket (20) is internally wrapped with an intestinal assembly (17), the temperature control water jacket (20) heats and controls the temperature of the intestinal assembly (17) at any time so as to ensure that the experimental simulation temperature in an intestinal inner cavity (44) of the intestinal assembly (17) is always 37 ℃ or other stable temperatures which are additionally required to meet the requirements of the intestinal in-vitro substitution experiments, the bottom end of the intestinal assembly (17) is provided with a collection outlet (4) and is connected into a collection bottle (65) through a collection liquid pipe (64) and a collection valve (52), the upper opening (25) of the intestinal cavity at the uppermost end of the intestinal assembly (17) is provided with a large sealing cover (31) to seal and, the large sealing cover (31) is provided with an air release port (27) with a pressure regulating air valve (26), oxygen is discharged after nitrogen is filled, an anaerobic environment is formed in the intestinal assembly (17), various reagents and fluid or liquid samples are injected into an intestinal inner cavity (44) of the intestinal assembly (17) through a sample adding port (29) of the large sealing cover (31) for experiments, the fluid or liquid samples can also be extracted at any time for assay analysis, the intestinal assembly simulates the large intestine of a human or animal, the large intestine is provided with a plurality of annular bags (36) and annular folds (42) to form a dome-shaped intestinal wall (16), the inner part of the intestinal inner cavity (44) is used for accommodating various reagents and experimental samples to carry out in-vitro substitution experiments in the simulated intestinal tract, a water jacket temperature sensor (34), a temperature sensor (43), an acid-base sensor (40), a pressure sensor (38) and an oxygen sensor (33) are sequentially arranged on two sides of the annular bags (36) on the upper side of the dome-shaped intestinal wall (16), and are connected to a main control lead (60) Experimental data information in the temperature control water jacket (20) and the intestinal assembly (17) is sent, and meanwhile, the main control computer (60) controls a nitrogen gas inlet (45), an alkali liquor inlet (11), an acid liquor inlet (13), a nutrient solution inlet (15) and a gas metering pump (47), a liquid metering pump (10), a collection valve (52), a water bath pump (7), an electric control warmer (51) and a swing motor (62) of a standby inlet (19) with an inlet cover plug (18) through a control lead (41) to maintain the requirements of an anaerobic environment, pH value and nutrient solution in an intestinal cavity (44) of the intestinal assembly (17) during experiment so as to meet the requirements of an intestinal in-vitro substitution experiment.
2. The dynamic rocking intestinal tract in-vitro substitution experimental device according to claim 1, which is characterized in that: the intestinal assembly (17) is an irregular thin-wall container which is made of a firm acid-resistant and alkali-resistant material and simulates the shape of a human or animal large intestine, the container is wrapped by a temperature-controlled water jacket (20) for controlling temperature, the bottom end of a funnel-shaped intestinal cavity lower opening (49) at the bottommost end of the intestinal assembly (17) is provided with a collection outlet (4) which is connected into a collection bottle (65) arranged on a rigid bedplate (58) of a support bench (59) through a collection liquid pipe (64) and a collection valve (52), the uppermost end of the intestinal assembly (17) is provided with an intestinal cavity upper opening (25) with an external thread buckle, a large sealing cover (31) with an internal thread buckle is assembled, various reagents and experimental samples are put into or taken out from the large sealing cover (31), a plurality of circular ring-like bags (36) with continuous bulges and a plurality of circular rings and circular ring-like folds (42) at the connection part are seamlessly connected between the intestinal cavity lower opening (49) and the intestinal cavity upper opening (25) to form a closed intestinal inner cavity (44), various reagents and experimental samples are contained to carry out an in-vitro substitution experiment in a simulated intestinal canal, a nitrogen inlet (45), an alkali liquor inlet (11), an acid liquor inlet (13), a nutrient solution inlet (15), a standby inlet (19) with an inlet cover plug (18), a water jacket temperature sensor (34), a temperature sensor (43), an acid-base sensor (40), a pressure sensor (38) and an oxygen sensor (33) are sequentially arranged at two sides of a quasi-annular bag (36) on the upper side of a dome-shaped intestinal wall (16), the nitrogen inlet (45) is filled with nitrogen from a nitrogen pressure bottle (56) arranged on a rigid bedplate (58) of a supporting bench (59) through a gas transmission elbow pipe (50) under the control of a one-way valve B (46) and a gas type metering pump (47) and forces an air release port (27) arranged on a large sealing cover (31) to discharge oxygen under the control of a pressure regulating air valve (26) so as to form an oxygen-free environment in an intestinal canal inner cavity, an alkali liquor inlet (11), an acid liquor inlet (13) and a nutrient solution inlet (15) are respectively pumped and filled into an intestinal inner cavity (44) of an intestinal assembly (17) from an alkali liquor bottle (1), an acid liquor bottle (2) and a nutrient solution bottle (3) which are arranged on a rigid bedplate (58) of a supporting bench (59) through respective infusion bent pipes (5) under the control of respective one-way valves A (14) and liquid metering pumps (10) to adjust the pH value and the nutrient solution requirement so as to meet various requirements of intestinal in-vitro substitution experiments, the liquid metering pump (10), the gas metering pump (47), a temperature sensor (43), an acid-base sensor (40), a pressure sensor (38), an oxygen sensor (33) and a collecting valve (52) are connected to a main control computer (60) through control leads (41) to be controlled in an internal connection manner, the exterior of the bottommost end of the intestinal assembly (17) is fixedly connected with a water jacket lower wall (8) of a temperature control water jacket (20) in a, the outer part of the uppermost end of the intestinal assembly (17) is fixedly connected with the water jacket upper wall (32) of the temperature control water jacket (20) in a sealing way, and forms a circle with the water jacket side wall (39) of the temperature control water jacket (20) to form a closed water bath inner chamber (12), and the temperature in the intestinal inner chamber (44) of the intestinal assembly (17) is controlled to ensure that the experimental simulation temperature in the intestinal inner chamber (44) of the intestinal assembly (17) is always 37 ℃ or other stable temperatures with other requirements are met to meet the requirements of intestinal in-vitro substitution experiments.
3. The dynamic rocking intestinal tract in-vitro substitution experimental device according to claim 1, which is characterized in that: the large sealing cover (31) is a circular cover made of an acid-base corrosion resistant material, an upper sealing rubber ring (24) and an internal thread buckle matched with the external thread of the upper opening (25) of the intestinal cavity of the intestinal assembly (17) are arranged on the inner wall of the cover, the internal and external environments of the intestinal cavity (44) of the intestinal assembly (17) are sealed and isolated after being screwed, two round holes with sealing rubber rings and different inner diameters are formed in the upper surface of the large sealing cover (31), an air discharge port (27) with a pressure regulating air valve (26) is arranged on the round hole with the small inner diameter, oxygen is discharged under the control of the pressure regulating air valve (26) after the intestinal assembly (17) is filled with nitrogen so as to form an anaerobic environment in the intestinal cavity (44) of the intestinal assembly (17), a sample adding port (29) with a small sealing cover (30) is arranged in the round hole with the large inner diameter, and various reagents and fluid or liquid samples required by experiments are injected into the intestinal cavity (44) of the intestinal assembly (17) at any time for experiments, and fluid or liquid samples can be extracted from the intestinal cavity (44) of the intestinal assembly (17) at any time for assay analysis, and the air release port (27) on the large sealing cover (31) and the sample injection port (29) on the small sealing cover (30) can be adjusted up and down in position so as to adapt to different sample volumes and property requirements during experiments.
4. The dynamic rocking intestinal tract in-vitro substitution experimental device according to claim 1, which is characterized in that: the temperature control water jacket (20) is a round tube-shaped closed container made of firm transparent heat-resistant materials, the uppermost edge of the temperature control water jacket (20) is a round sheet-shaped water jacket upper wall (32) with a round opening at the center, the center round opening of the water jacket upper wall (32) is in seamless connection with an upper opening (25) of an intestinal cavity of the intestinal assembly (17), the excircle of the water jacket upper wall (32) is in seamless connection with the upper end of a round tube-shaped water jacket side wall (39), a circle of the lower end of the water jacket side wall (39) is in seamless connection with a round sheet-shaped water jacket lower wall (8), an inner closed cavity-shaped water bath inner chamber (12) is formed between the temperature control water jacket (20) and the intestinal assembly (17), a plurality of fixed support legs (37) are respectively arranged on two edges of the upper end and the lower end of the water jacket side wall (39) to fix the whole water jacket (20) and the intestinal temperature control assembly (17) therein on a, a water jacket temperature sensor (34) connected with a main control computer (60) through a control lead (41) is fixedly arranged on the side wall (39) of the water jacket to monitor the temperature of the temperature-controlled hot water in the water bath inner chamber (12), two sides of the water jacket lower wall (8) are respectively provided with a water jacket interface (48) which is vertically opened downwards, the temperature-controlled hot water generated by the electric control warmer (51) is sent into a water bath inner cabin (12) of the temperature-controlled water jacket (20) to heat and control the temperature of the intestinal assembly (17) at any time so as to ensure that the experimental simulation temperature in the intestinal inner cavity (44) of the intestinal assembly (17) is always 37 ℃ or other stable temperatures which are additionally required to meet the requirement of an intestinal in-vitro substitution experiment.
5. The dynamic rocking intestinal tract in-vitro substitution experimental device according to claim 1, which is characterized in that: the swing mechanism (22) is a mechanical part which is made of rigid insulating materials and can swing and support the temperature control water jacket (20) and the intestinal assembly (17), the main element of the swing mechanism (22) is a movable wall plate (23) made of an approximately elliptical thin plate, the edge of the upper end of the movable wall plate (23) is provided with a semicircular constraint chute (21) with a long and narrow opening, a thin shaft-shaped constraint protective shaft (28) which is vertically arranged at the upper end of a rear wall plate (35) of the support rack (59) penetrates through the constraint chute (21) to play a role of freely constraining the movable wall plate (23) to slide and swing left and right on the support rack (59), the lower end of the movable wall plate (23) extends out a small semicircular plate (55), a support rotating shaft (54) which can freely rotate is arranged on the circle center of the semicircular plate (55), and the support rotating shaft (54) penetrates through the circle center of the semicircular plate (55) of the swing mechanism (22) to support the movable wall plate (23) on the The rear wall plate (35) of the supporting rack (59) swings freely in the restraint range of the restraint guard shaft (28), a swing motor (62) which is connected to a main control computer (60) through a control lead (41) and is provided with a driving gear (63) is fixedly arranged at the middle position of the lower part of the rear wall plate (35) through a motor support (61), driven gears (53) are uniformly distributed on the excircle of a semicircular moving plate (55) and are meshed with the driving gear (63) of the lower swing motor (62), the whole temperature control water jacket (20) and an intestinal assembly (17) in the movable wall plate (23) are fixedly fixed through a plurality of fixed support legs (37), when the swing motor (62) rotates forwards and backwards under the control of the main control computer (60), the driving gear (63) drives the semicircular moving plate (55) to rotate forwards and backwards, and the elliptic movable wall plate (23) is driven by the semicircular moving plate (55) to rotate left and right under the restraint of the supporting rotating shaft (54) and the restraint guard shaft (28) The whole temperature control water jacket (20) and the intestinal assembly (17) in the temperature control water jacket are driven to swing left and right by swinging to complete the function of simulating intestinal peristalsis, and the main control computer (60) can set the swinging frequency and amplitude in advance to carry out various dynamic intestinal in-vitro substitution experiments.
6. The dynamic rocking intestinal tract in-vitro substitution experimental device according to claim 1, which is characterized in that: the supporting rack (59) is a table-type bracket which is made of firm corrosion-resistant rigid materials and can support a plurality of functional components, the bottommost part of the supporting rack (59) is a planar rigid bedplate (58) with elastic foot pads (57), and a fixed rear wall plate (35) is vertically arranged at the rear part of the rigid bedplate (58).
7. The dynamic rocking intestinal tract in-vitro substitution experimental device according to claim 1, which is characterized in that: the main control computer (60) consists of a computer hardware system special for scientific research, a special software program system for intestinal tract in vitro substitution experiment related specialties and an execution system connected to the intestinal tract assembly (17), the swing mechanism (22) and each electric appliance element to be used on the support rack (59), the computer hardware system and the special software program system are combined in the main control computer (60), the main control computer (60) is arranged on a rigid platform plate (58) of the support rack (59) and is sequentially connected to a water jacket temperature sensor (34), a temperature sensor (43), an acid-base sensor (40), a pressure sensor (38) and an oxygen sensor (33) through a control lead (41) of the execution system to perform data arrangement on the temperature of the temperature control water jacket (20) received by the execution system and various parameters of an experiment sample in an intestinal tract inner cavity (44) of the intestinal tract assembly (17), The device comprises a parameter analysis, a storage record and a timely feedback main control computer (60), and sequentially controls and drives a nitrogen inlet (45), an alkali liquor inlet (11), an acid liquor inlet (13), a nutrient liquor inlet (15) and a gas metering pump (47), a liquid metering pump (10), a collection valve (52) which are arranged on a dome-shaped intestinal wall (16) of an intestinal assembly (17), a water bath pump (7), an electric control heater (51) and a swing motor (62) which are arranged on a movable wall plate (23) of a swing mechanism (22) and a rear wall plate (35) of a support rack (59) through a control lead (41) to work, so that the whole dynamic intestinal in-vitro substitution experiment is realized.
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