CN105702146A - Bionic dynamic mouse stomach-duodenum digestive system simulation device and simulation experiment method - Google Patents
Bionic dynamic mouse stomach-duodenum digestive system simulation device and simulation experiment method Download PDFInfo
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Abstract
The invention relates to a bionic dynamic mouse stomach-duodenum digestive system simulation device and simulation experiment method. The bionic dynamic mouse stomach-duodenum digestive system simulation device includes a stomach digestive system, a duodenum digestive system, a feeding-emptying device and a heat preservation box, the feeding-emptying device conveys gastric juice towards the stomach digestive system, and conveys bile and pancreatic juice towards the duodenum digestive system, and the stomach digestive system and the duodenum digestive system are communicated with each other. The bionic dynamic mouse stomach-duodenum digestive system simulation experiment method includes the steps of: (1) adjusting temperature in the heat preservation box; (2) filling gastric juice, pancreatic juice and bile; (3) preparing a food sample; (4) injecting gastric juice to a bionic mouse stomach model; (5) injecting the food sample to the bionic mouse stomach model; (6) simulating a digestion process; and (7) recording the digestion process according to periods of time. The bionic dynamic mouse stomach-duodenum digestive system simulation experiment method has a real simulation result, the digestion process is extremely similar to a digestion process of a living mouse, and thus the method has very good application value.
Description
Technical field
The present invention relates to bionical dynamic Mus stomach-duodenum digestive system analog and analogue experiment method, this digestive system analog can be used for the food of bionical number of different types and structure in vitro and medicine in the mixing of true Mus gastric, grinding, digestion and evacuation procedure。
Background technology
Current any new functional food and medicine are before putting goods on the market and being trusted by consumer and patient and accept, it is necessary to carry out experiment and the evaluation of the aspects such as strict nutritive value, toxicity, pharmacology。And to assess its nutrient substance and the effective active composition release rate in gastrointestinal tract and utilization rate, first have to understand them in the digestion of gastric and evacuation procedure, recognize its nutrient delivery process in vivo, thus improving the processing method of food and medicine or building new functional food, it is effectively improved digesting and assimilating and the utilization rate of medicine of nutritional labeling。Additionally, along with food-safety problem becomes increasingly conspicuous, this is to effective, quickly, detection food additive and poisonous and harmful element residual technology in vivo propose new challenge easily。
The digestion in the gastrointestinal tract of Study of functional food and medicine, emptying and effective active forming process, and the residual that poisonous and harmful substance is in vivo, its traditional method mainly resorts to living animal and human volunteer's clinical trial。Owing to the selection of live body or clinical experiment object is restricted, and because bion physiology (including sex, age, medical history etc.) difference, architectural difference and the environmental factors impact on organism state, cause live body and clinical trial to be wasted time and energy, cost is high, poor reproducibility, and also suffers from the restriction of ethics aspect。External Bionic digestion road, it it is the device that carries out human simulation of the hydrodynamic behavior in the digestive system to human body or animal and digestive environments, digestive tract, not only there is no the restriction of ethics aspect, and live body and clinical trial can be replaced wholly or in part, or come test situation and optimization live test condition in predictor as " front test ", thus reduce cost, saving time, improve repeated and accuracy purpose。
In recent years, researcheres develop multiple external digestion system model, for studying physicochemical change in food digestion process, the interphase interaction of food, beneficial bacteria of intestinal tract survival rate, the development and application of functional food and medicine and the poisonous and harmful substance residual in gastrointestinal tract and metabolic process。Current external digestion system model is divided into rigid system, semi-rigid system and flexiblesystem according to the big I of elasticity。The outer stomach of rigid body or gastrointestinal model utilize the simple receptacle such as beaker to drive gastric content to mix by stirring apparatus or shaking table concussion equipment, simplicity is cheap, drive (rotating speed) controlled, but lacks the effectively simulation to stomach or the physiology and appearance of intestinal, stomach or enteral physicochemical environment, coat of the stomach or intestinal wall motion and hydrodynamics behavior。Thus, Recent study persons are more likely to study and have harmonization of the stomach intestinal physiology morphological characteristic, coat of the stomach and intestinal wall is movable, gastric juice, intestinal juice and chyme is secreted continuously and emptying etc. is increasingly complex, the outer bionical peptic digestion system of dynamic body closer to true harmonization of the stomach intestinal。The HGS (HumanGastricSimulator) of TIM (TNOIntestinalModel) with Food Research Inst. of DGM (DynamicGastricModel) that current Application comparison external digestion system widely mainly includes being developed by Leeds, England Hai De Food Research Inst., Holland's TNO nutrition and Univ California-Davis USA。The classical external digestion system of these three is respectively arranged with quality, it is possible to the effectively physical and chemical factor in Gl tract, the digestion in the gastrointestinal tract of reconstructing portion food or medicine, emptying and effective active composition release behavior。But they do not possess the details (size, gastric wall fold etc.) of the form of true stomach or intestinal and physiological structure, it is impossible to reappear food in the distribution of true gastric and digestion, purge sequence, and lack the effective simulation to coat of the stomach or intestinal wall peristaltic contraction。Additionally, these systems structure complexity, cost is higher, and dismounting and cleaning are inconvenient；Owing to ethics restriction cannot conveniently obtain the physiologic parameters that human gastrointestinal tract is relevant, cause effectiveness and the science that can not effectively verify said system。
For this, build a kind of form with true harmonization of the stomach intestinal and physiology details, can effectively simulate coat of the stomach and intestinal wall peristaltic contraction and low cost, dynamic bionic simple to operate stomach-duodenum digestive system will be with a wide range of applications。
Summary of the invention
In order to solve above-mentioned Problems existing, the invention discloses bionical dynamic Mus stomach-duodenum digestive system analog and analogue experiment method, its concrete technical scheme is as follows:
Bionical dynamic Mus stomach-duodenum digestive system analog, this analog is for simulating foodstuff samples digestion in duodenum then through pancreatic juice and bile after the stomach back warp of Mus crosses Gastric juice digestion, for studying in digestion process and the change of final chyme, this analog includes peptic digestion system, duodenum digestive system, stream adds-emptier and incubation chamber, described peptic digestion system, duodenum digestive system and stream adds-and emptier is respectively positioned in incubation chamber, stream adds-and emptier carries gastric juice towards peptic digestion system, bile and pancreatic juice is carried towards duodenum digestive system, peptic digestion system and duodenum digestive system are interconnected,
Described peptic digestion system includes bionic-mouse stomach model and electric compressor-rolling extrusion apparatus, equipped with foodstuff samples in described bionic-mouse stomach model, described electric compressor-rolling extrusion apparatus is used for rolling extrusion bionic-mouse stomach model, and bionic-mouse stomach model produces simulation stomach and shrinks-roll digestion action；
Described duodenum digestive system includes bionical duodenum model pipe and electric peristaltic extruding apparatus, foodstuff samples in described bionic-mouse stomach model is discharged in bionical duodenum model pipe after bionic-mouse stomach model digests, described electric peristaltic extruding apparatus is used for the bionical duodenum model pipe of extruding of wriggling, and bionical duodenum model pipe produces the action of simulation duodenum wriggling digestion；
Described stream adds-and emptier includes single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, described single channel syringe pump A is by gastric juice pipe connection bionic-mouse stomach model, single channel syringe pump A carries gastric juice in bionic-mouse stomach model, described single channel syringe pump B connects bionical duodenum model pipe by pancreatic juice pipe, single channel syringe pump C connects bionical duodenum model pipe by bile pipe, single channel syringe pump B carries pancreatic juice in bionical duodenum model pipe, and single channel syringe pump C carries bile in bionical duodenum model pipe；
The free end of described bionical duodenum model pipe is provided with chyme receiving flask, and described chyme receiving flask is for collecting the chyme discharged from bionical duodenum model pipe。
Described bionic-mouse stomach model is utilize the Mus stomach that real full enlarged abdomen is little to make molding by overmolded, uses " DragonSkin?" addition-type silicon rubber is made, the size of described bionic-mouse stomach model, shape is consistent with true rat stomach with internal structure, with boundary ridge for boundary, it is divided into glandular stomach and glandular stomach, glandular stomach inner wall smooth, glandular stomach inwall is full of fold, glandular stomach connection esophagus, silica gel valve pipe is installed in esophagus, glandular stomach connection pyloric canal and gastric juice secretory duct, described gastric secretion pipe connection single channel syringe pump A, described pyloric canal connects bionical duodenum model pipe, foodstuff samples enters bionic-mouse stomach model from esophagus, food becomes chyme after bionic-mouse stomach model digests and is expelled to bionical duodenum model pipe from pyloric canal。
Described electric compressor-rolling extrusion apparatus includes motor, support, eccentric, frequency controller, gusset, belt, belt pulley, bevel gear, pressing plate, fixing plate, gripper shoe and base, described support is fixed on base, described support is provided with two fixing plates, it is fixed with gripper shoe between the fixing plate of said two, described bionic-mouse stomach model is installed on the supporting plate, each fixing plate is provided with a motor, bevel gear connecting eccentric wheel is passed through in one end of motor, glandular stomach is carried out compression vertical up and down with dynamic pressure plate by eccentric rotation process, described fixing plate is additionally provided with three belt pulleys of distribution triangular in shape, described belt pulley is cased with belt, one of them belt pulley has step motor drive, motor drives belt and then drives eccentric glandular stomach to be carried out from gastric antrum toward pylorus direction rolling extrusion, described frequency controller is connected with motor, frequency controller regulates bionic-mouse stomach model by the rotating speed of control motor and is subject to compression and rolling extrusion frequency and amplitude。
Length and the internal diameter of described bionical duodenum model pipe are equal with the duodenal length of true Mus and internal diameter size, pyloric canal and bionical duodenum model pipe are connected by the four-way pipe of " K " word shape, one end of " I " part of the four-way pipe of described " K " word shape is connected with pyloric canal, the other end is connected with bionical duodenum model pipe, one end of " < " part of the four-way pipe of " K " word shape is connected with single channel syringe pump B by pancreatic juice pipe, and the other end is connected with single channel syringe pump C by bile pipe。
Described external electric wriggling extruding apparatus includes motor, eccentric, frequency controller, bevel gear, fast pulley, gripper shoe, fixing plate, belt, belt pulley and base, described support is fixed on base, fixing plate on described support, described fixing plate arranges several fast pulleys, described bionical duodenum model pipe is curved through each fast pulley successively, the side of described fixing plate is provided with two motors, described every motor is each through bevel gear connecting eccentric wheel, bionical duodenum model pipe is carried out the wriggling extruding of subsection interleaving formula by described eccentric, described frequency controller connects motor, frequency controller carrys out the eccentric adjustment wheel wriggling extruding frequency to bionical duodenum model pipe by controlling the rotating speed of motor。
The chyme upstream end of described bionical duodenum model pipe is provided with peristaltic pump。
Be provided with in described incubation chamber in heating heat-preserving box air heating lamp and for being illuminated illuminating lamp in incubation chamber, being additionally provided with the digital display temperature controller for monitoring the real time temperature within incubation chamber in described incubation chamber, the side of described incubation chamber is provided with sliding door。
Apply the analogue experiment method of any of the above-described described bionical dynamic Mus stomach-duodenum digestive system analog, comprise the steps:
(1) adjust the room temperature in incubation chamber: open the heating lamp in incubation chamber and digital display temperature controller, when the temperature in incubation chamber maintains 37 DEG C, close heating lamp；
(2) fill gastric juice, pancreatic juice and bile: take a gastric juice pipe, the full gastric juice of fill wherein, then one end of gastric juice pipe is connected with single channel syringe pump A, the other end is connected with pyloric canal, separately take a pancreatic juice pipe and a bile pipe, the full pancreatic juice of fill in pancreatic juice pipe, one end of pancreatic juice pipe is connected with single channel syringe pump B, the full bile of fill in bile pipe, one end of bile pipe is connected with single channel syringe pump C, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
(3) foodstuff samples is prepared: take food and be placed in equipped with, in the container of deionized water, after being sufficiently stirred for, adding Mus saliva, then magnetic agitation, to simulate food at the intraoral digestion process of Mus；
(4) injecting gastric juice to bionic-mouse stomach model: inject simulated gastric fluid with syringe to bionic-mouse stomach model, during to simulate rat stomach fasting state, gastric juice remains；
(5) inject foodstuff samples to bionic-mouse stomach model: be expelled to bionic-mouse stomach model by the foodstuff samples prepared in step (3) from esophagus is disposable, silica gel valve pipe is connected with esophagus outlet, it is prevented that gastric food overflows, and keeps gastric voltage stabilizing；
(6) digestion process of simulation bionic-mouse stomach model and bionical duodenum model pipe: initiation culture controller, electric compressor-rolling extrusion apparatus starts the glandular stomach of bionical rat stomach model is compressed vertically, simultaneously to glandular stomach from gastric antrum toward the rolling extrusion in pylorus direction, by single channel syringe pump A, gastric juice is pushed in bionic-mouse stomach model, by single channel syringe pump B, pancreatic juice is pushed in bionical duodenum model pipe, by single channel syringe pump C, bile is pushed in bionical duodenum model pipe, pancreatic juice and bile and the chyme from the discharge of bionic-mouse stomach model are transported to bionical duodenum model pipe by peristaltic pump, bionical duodenum model pipe is carried out segmentation wriggling extruding by electric peristaltic extruding apparatus, chyme is discharged from bionical duodenum model pipe the most at last, it is collected bottle to collect；
(7) time segment record digestion process: in step (6) described digestion process, digestion process is divided into several time periods, stops a frequency controller in each time period, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, takes out bionic-mouse stomach model and bionical duodenum model pipe, collects the digest in bionic-mouse stomach model and bionical duodenum model pipe respectively, measures respectively and record its pH, maltose concentration, gastric emptying rate, storage modulus, loss modulus, dynamic viscosity and apparent viscosity, and observe microstructure, then chyme is put back to again in bionic-mouse stomach model and bionical duodenum model pipe, by bionic-mouse stomach model and bionical duodenum model pipe retrieval device, open frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, continues experiment, until chyme is all discharged from bionical duodenum model pipe, and measures and the final pH discharging chyme of record, maltose concentration, gastric emptying rate, storage modulus, loss modulus, dynamic viscosity and apparent viscosity, and observe microstructure；
(8) clean bionic-mouse stomach model and bionical duodenum model pipe: after experiment terminates, clean bionic-mouse stomach model and bionical duodenum model pipe in time with deionized water。
In described step (7), microstructure is observed by laser confocal microscope CSLM and scanning electron microscope sem。
The operation principle of the present invention is:
The present invention, utilizes the specification of Mus stomach under real full abdomen state to make the paraffin mould of bionic-mouse stomach model, and the bionic-mouse stomach model made can simulate Mus stomach comparatively truly。The present invention, utilizes electric compressor-rolling extrusion apparatus that the glandular stomach that carries out of bionic-mouse stomach model is carried out compression vertical up and down, glandular stomach is carried out from gastric antrum toward pylorus direction rolling extrusion, can simulate Mus stomach more realistically and digest action really。
The present invention, the specification of bionical duodenum model pipe is consistent with the duodenum specification of true Mus, improves the verity of simulation experiment。The present invention, utilizes external electric wriggling extruding apparatus that bionical duodenum model pipe carries out extruding of wriggling, simulates chyme digestion change procedure in Mus duodenum。
The present invention, by the foodstuff samples after Mus salivary digestion successively bionic-mouse stomach model and bionical duodenum model pipe, it is possible to simulation foodstuff samples continuous print digestion process in the harmonization of the stomach duodenum of Mus more realistically。
The invention has the beneficial effects as follows:
1. owing to including peptic digestion system and the duodenum digestive system two large divisions of Mus, thus the present invention can more comprehensively simulate the food complete digestion process in harmonization of the stomach duodenum by ratio, it is possible to the digestive environments dynamically and in the true rat stomach of accurate reproduction；
2. bionic-mouse stomach model adopts the addition-type silicon rubber raw material of high resiliency, high stability, the high grade of transparency, is made by true Mus stomach 1:1 overmolded, possesses the details (inwall fold etc.) of the form of true rat stomach, size and physiological structure；
3. bionical duodenum model pipe adopts silica gel hose to make, and its length is similar with true Mus duodenum with internal diameter size；
4. electric compressor-rolling extrusion apparatus and external electric are wriggled the reproduction true Mus stomach and duodenal peristaltic contraction frequency and amplitude that extruding apparatus all can highly be similar to, and can simulate the peristaltic contraction form of true Mus stomach coat of the stomach and intestinal wall；
5. adopt single channel syringe pump A, single channel syringe pump B, single channel syringe pump C and peristaltic pump, it is possible to the secreting rate of accurate simulated gastric fluid, pancreatic juice and bile and the rate of evacuation of chyme；
6. volume of the present invention is little, low cost, simple to operate, easy to clean, it is possible to for the digestion of food and medicine is carried out in vitro study。
Accompanying drawing explanation
Fig. 1 is the bionic-mouse stomach model structural representation of the present invention,
Fig. 2 is the peptic digestion system structure schematic diagram of the present invention,
Fig. 3 is the E direction view of Fig. 2,
Fig. 4 is the F direction view of Fig. 3,
Fig. 5 is the duodenum digestive system structural representation of the present invention,
Fig. 6 is the G direction view of Fig. 5,
Fig. 7 is the H direction view of Fig. 5,
Fig. 8 is the structural representation of the present invention,
Reference numerals list: 1 glandular stomach, 2 glandular stomaches, 3 pylorics canal, 4 esophaguses, 5 gastric juice pipes, 6 four-way pipes, 7 pancreatic juice pipes, 8 bile pipes, 9 bionical duodenum model pipe, 10 bases, 11 supports, 12 fixing plates, 13 eccentrics, 14 bionic-mouse stomach model, 15 bevel gears, 16 belt pulleys, 17 belts, 18 motors, 19 peristaltic pumps, 20 single channel syringe pump A, 21 single channel syringe pump B, 22 single channel syringe pump C, 23 frequency controllers, 24 chyme receiving flasks, M electric compressor-rolling extrusion apparatus, N electric peristaltic extruding apparatus。
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, it is further elucidated with the present invention。It should be understood that following detailed description of the invention is merely to illustrate the present invention rather than restriction the scope of the present invention。
Fig. 8 is the structural representation of the present invention, and in figure, accompanying drawing is labeled as: 19 peristaltic pumps, 20 single channel syringe pump A, 21 single channel syringe pump B, 22 single channel syringe pump C, 23 frequency controllers, 24 chyme receiving flasks, M electric compressor-rolling extrusion apparatus, N electric peristaltic extruding apparatus。Visible in conjunction with accompanying drawing, this bionical dynamic Mus stomach-duodenum digestive system analog, this analog is for simulating food digestion in duodenum then through pancreatic juice and bile after the stomach back warp of Mus crosses Gastric juice digestion, for studying in digestion process and the change of final chyme, this analog includes peptic digestion system, duodenum digestive system, stream adds-emptier and incubation chamber, described peptic digestion system, duodenum digestive system and stream adds-and emptier is respectively positioned in incubation chamber, stream adds-and emptier carries gastric juice towards peptic digestion system, bile and pancreatic juice is carried towards duodenum digestive system, peptic digestion system and duodenum digestive system are interconnected, the present invention, food sequentially passes through peptic digestion system and duodenum digestive system, imitate the digestion of this two big digestive system。
Described peptic digestion system includes bionic-mouse stomach model and electric compressor-rolling extrusion apparatus, equipped with food in described bionic-mouse stomach model, described electric compressor-rolling extrusion apparatus is used for rolling extrusion bionic-mouse stomach model, and bionic-mouse stomach model produces simulation stomach and shrinks-roll digestion action；Electric compressor-rolling extrusion apparatus rolling extrusion bionic-mouse stomach model, simulation bionic-mouse stomach model is from line crawl digestion process。
Described duodenum digestive system includes bionical duodenum model pipe and electric peristaltic extruding apparatus, food in described bionic-mouse stomach model is discharged in bionical duodenum model pipe after bionic-mouse stomach model digests, described electric peristaltic extruding apparatus is used for the bionical duodenum model pipe of extruding of wriggling, and bionical duodenum model pipe produces the action of simulation duodenum wriggling digestion；Bionical duodenum model pipe is extruded by electric peristaltic extruding apparatus, simulates the digestion process voluntarily of bionical duodenum model pipe。
Described stream adds-and emptier includes single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, described single channel syringe pump A is by gastric juice pipe connection bionic-mouse stomach model, single channel syringe pump A carries gastric juice in bionic-mouse stomach model, described single channel syringe pump B connects bionical duodenum model pipe by pancreatic juice pipe, single channel syringe pump C connects bionical duodenum model pipe by bile pipe, single channel syringe pump B carries pancreatic juice in bionical duodenum model pipe, and single channel syringe pump C carries bile in bionical duodenum model pipe；Gastric juice, pancreatic juice and bile are in order to meet the more real digestion process of simulation。
Stream adds-emptier described in single channel syringe pump A, single channel syringe pump B and single channel syringe pump C produced by Baoding LanGe constant flow pump Co., Ltd, model is TJ-3A/W0109-1B, can installing the multiple standards syringe of mL specification, its linear velocity ranges for 7.94 μm of .min-1-79.4mm.min-1。
The free end of described bionical duodenum model pipe is provided with chyme receiving flask, and described chyme receiving flask is for collecting the chyme discharged from bionical duodenum model pipe。Receiving flask collects chyme, not only prevents chyme from polluting laboratory table, also allows for studying further chyme。
Fig. 1 is the bionic-mouse stomach model structural representation of the present invention, and accompanying drawing is labeled as: 1 glandular stomach, 2 glandular stomaches, 3 pylorics canal, 4 esophaguses, 5 gastric juice pipes, 6 four-way pipes, 7 pancreatic juice pipes, 8 bile pipes, 9 bionical duodenum model pipe。Visible in conjunction with this accompanying drawing, described bionic-mouse stomach model is utilize the Mus stomach that real full enlarged abdomen is little to make molding by overmolded, uses " DragonSkin?" addition-type silicon rubber is made, the size of described bionic-mouse stomach model, shape is consistent with true rat stomach with internal structure, with boundary ridge for boundary, it is divided into glandular stomach and glandular stomach, glandular stomach inner wall smooth, glandular stomach inwall is full of fold, glandular stomach connection esophagus, length of esophagus is 5cm, silica gel valve pipe is installed in esophagus, glandular stomach connection pyloric canal and gastric juice secretory duct, described gastric secretion pipe connection single channel syringe pump A, described pyloric canal connects bionical duodenum model pipe, food enters bionic-mouse stomach model from esophagus, it is expelled to bionical duodenum model pipe from pyloric canal after bionic-mouse stomach model is digested to chyme。
" DragonSkin?" high-performance A, B two-component addition-type silicon rubber, use the bionical rat stomach silicone rubber model that this material makes to be translucent, do not dissolve in, not with acid-base reaction, inviscid, tensile strength up to 475psi, losing External Force Acting resilience can recover former state rapidly。
The length of bionic-mouse stomach model × wide × height is respectively as follows: 40 (± 3) × 30(± 2) × 21(± 2) mm, maximum water storage volume is 9.0 ± 0.8mL。
The wall of stomach thickness of glandular stomach and glandular stomach respectively 0.65 ± 0.15mm and 1.22 ± 0.25mm。
The internal diameter of esophagus and external diameter are 3.0 ± 0.2mm and 5.0 ± 0.2mm respectively。
The internal diameter of silica gel valve pipe and external diameter are 4.8 ± 0.2mm and 7.0 ± 0.2mm respectively, and inside has two panels valve to stick at valve inside pipe wall with " V " type, and two panels valve commissure place contacts with each other but adhesion。
Fig. 2 is the peptic digestion system structure schematic diagram of the present invention, and Fig. 3 is the E direction view of Fig. 2, and Fig. 4 is the F direction view of Fig. 3, and in figure, accompanying drawing is labeled as: 10 bases, 11 supports, 12 fixing plates, 13 eccentrics, 14 bionic-mouse stomach model, 15 bevel gears, 16 belt pulleys, 17 belts, 18 motors。Visible in conjunction with this three width accompanying drawing, described electric compressor-rolling extrusion apparatus includes motor, support, eccentric, frequency controller, gusset, belt, belt pulley, bevel gear, pressing plate, fixing plate, gripper shoe and base, described support is fixed on base, described support is provided with two fixing plates, it is fixed with gripper shoe between the fixing plate of said two, described bionic-mouse stomach model is installed on the supporting plate, each fixing plate is provided with a motor, bevel gear connecting eccentric wheel is passed through in one end of motor, glandular stomach is carried out compression vertical up and down with dynamic pressure plate by eccentric rotation process, described fixing plate is additionally provided with three belt pulleys of distribution triangular in shape, described belt pulley is cased with belt, one of them belt pulley has step motor drive, motor drives belt and then drives eccentric glandular stomach to be carried out from gastric antrum toward pylorus direction rolling extrusion, described frequency controller is connected with motor, frequency controller regulates bionic-mouse stomach model by the rotating speed of control motor and is subject to compression and rolling extrusion frequency and amplitude。
Described gusset, pressing plate, gripper shoe and base are all fine aluminum sheet material matter, and surface is through antirust treatment, and fixing plate is acrylic material, and thickness is 1.0 ± 0.2cm。
The rotating speed adjustable extent of described motor rotating speed is 0-10rpm。
Described compression amplitude adjustable extent is 0-10mm, and rolling extrusion adjustable amplitude ranges for 0-5mm。
Length and the internal diameter of described bionical duodenum model pipe are equal with the duodenal length of true Mus and internal diameter size, pyloric canal and bionical duodenum model pipe are connected by the four-way pipe of " K " word shape, one end of " I " part of the four-way pipe of described " K " word shape is connected with pyloric canal, the other end is connected with bionical duodenum model pipe, one end of " < " part of the four-way pipe of " K " word shape is connected with single channel syringe pump B by pancreatic juice pipe, and the other end is connected with single channel syringe pump C by bile pipe。
The internal diameter of described external bionical duodenum silica gel hose and external diameter respectively 3.0 ± 0.1mm and 6.0 ± 0.2mm。
Described K " internal diameter of " I " part silicone gel pipe of type four-way pipe and external diameter be 4.8 ± 0.2mm and 7.0 ± 0.2mm respectively；Internal diameter and the external diameter of " < " part silicone rubber tubule are 1.0 ± 0.1mm and 2.0 ± 0.1mm respectively；
The internal diameter of pancreatic juice pipe and bile pipe and external diameter respectively 3.0 ± 0.2mm and 5.0 ± 0.2mm。
Fig. 5 is the duodenum digestive system structural representation of the present invention, Fig. 6 is the G direction view of Fig. 5, Fig. 7 is the H direction view of Fig. 5, visible in conjunction with this three width accompanying drawing, described external electric wriggling extruding apparatus includes motor, eccentric, frequency controller, bevel gear, fast pulley, gripper shoe, fixing plate, belt, belt pulley and base, described support is fixed on base, fixing plate on described support, described fixing plate arranges several fast pulleys, described bionical duodenum model pipe is curved through each fast pulley successively, the side of described fixing plate is provided with two motors, described every motor is each through bevel gear connecting eccentric wheel, bionical duodenum model pipe is carried out the wriggling extruding of subsection interleaving formula by described eccentric, described frequency controller connects motor, frequency controller carrys out the eccentric adjustment wheel wriggling extruding frequency to bionical duodenum model pipe by controlling the rotating speed of motor。
Described gripper shoe and base are all fine aluminum sheet material matter, and surface is through antirust treatment, and fixing plate is acrylic material, and thickness is 1.0 ± 0.2cm。
The rotating speed adjustable extent of described motor rotating speed is 0-20rpm。
Described eccentric and fast pulley parallel arranged, four fast pulleys are vertically arranged, and four eccentrics are separately fixed at both sides around between two, and the silica gel hose in duodenum model is staggered to be clipped between eccentric and fast pulley。
The chyme upstream end of described bionical duodenum model pipe is provided with peristaltic pump。Peristaltic pump model is 104K/BT(steppermotor), Chongqing Jie Heng peristaltic pump company limited produce, it is possible to assembling two kinds of wall thickness flexible pipes of 1mm and 1.6mm, internal diameter 1～4.8mm, flow-control ranges for 0-140mL.min-1.
Be provided with in described incubation chamber in heating heat-preserving box air heating lamp and for being illuminated illuminating lamp in incubation chamber, being additionally provided with the digital display temperature controller for monitoring the real time temperature within incubation chamber in described incubation chamber, the side of described incubation chamber is provided with sliding door。Incubation chamber be shaped as cuboid, select PC plate to be made, length × wide × height is 85 × 55 × 50cm。The power of heating lamp is 100W, from room temperature (25oC) heating is to 37oC needs 15min。Sliding door makes by acrylic board cutting, long and width respectively 42cm and 50cm。
Apply the analogue experiment method of any of the above-described described bionical dynamic Mus stomach-duodenum digestive system analog, comprise the steps:
(1) adjust the room temperature in incubation chamber: open the heating lamp in incubation chamber and digital display temperature controller, when the temperature in incubation chamber maintains 37 DEG C, close heating lamp；
(2) fill gastric juice, pancreatic juice and bile: take a gastric juice pipe, the full gastric juice of fill wherein, then one end of gastric juice pipe is connected with single channel syringe pump A, the other end is connected with pyloric canal, separately take a pancreatic juice pipe and a bile pipe, the full pancreatic juice of fill in pancreatic juice pipe, one end of pancreatic juice pipe is connected with single channel syringe pump B, the full bile of fill in bile pipe, one end of bile pipe is connected with single channel syringe pump C, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
(3) foodstuff samples is prepared: take food and be placed in equipped with, in the container of deionized water, after being sufficiently stirred for, adding Mus saliva, then magnetic agitation, to simulate food at the intraoral digestion process of Mus；
(4) injecting gastric juice to bionic-mouse stomach model: inject simulated gastric fluid with syringe to bionic-mouse stomach model, during to simulate rat stomach fasting state, gastric juice remains；
(5) inject foodstuff samples to bionic-mouse stomach model: be expelled to bionic-mouse stomach model by the foodstuff samples prepared in step (3) from esophagus is disposable, silica gel valve pipe is connected with esophagus outlet, it is prevented that gastric food overflows, and keeps gastric voltage stabilizing；
(6) digestion process of simulation bionic-mouse stomach model and bionical duodenum model pipe: initiation culture controller, electric compressor-rolling extrusion apparatus starts the glandular stomach of bionical rat stomach model is compressed vertically, simultaneously to glandular stomach from gastric antrum toward the rolling extrusion in pylorus direction, by single channel syringe pump A, gastric juice is pushed in bionic-mouse stomach model, by single channel syringe pump B, pancreatic juice is pushed in bionical duodenum model pipe, by single channel syringe pump C, bile is pushed in bionical duodenum model pipe, pancreatic juice and bile and the chyme from the discharge of bionic-mouse stomach model are transported to bionical duodenum model pipe by peristaltic pump, bionical duodenum model pipe is carried out segmentation wriggling extruding by electric peristaltic extruding apparatus, chyme is discharged from bionical duodenum model pipe the most at last, it is collected bottle to collect；
(7) time segment record digestion process: in step (6) described digestion process, digestion process is divided into several time periods, stops a frequency controller in each time period, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, takes out bionic-mouse stomach model and bionical duodenum model pipe, collects the digest in bionic-mouse stomach model and bionical duodenum model pipe respectively, measures respectively and record its pH, maltose concentration, gastric emptying rate, storage modulus, loss modulus, dynamic viscosity and apparent viscosity, and observe microstructure, then chyme is put back to again in bionic-mouse stomach model and bionical duodenum model pipe, by bionic-mouse stomach model and bionical duodenum model pipe retrieval device, open frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, continues experiment, until chyme is all discharged from bionical duodenum model pipe, and measures and the final pH discharging chyme of record, maltose concentration, gastric emptying rate, storage modulus, loss modulus, dynamic viscosity and apparent viscosity, and observe microstructure；
(8) clean bionic-mouse stomach model and bionical duodenum model pipe: after experiment terminates, clean bionic-mouse stomach model and bionical duodenum model pipe in time with deionized water。
In described step (7), microstructure is observed by laser confocal microscope CSLM and scanning electron microscope sem。
Specific embodiment is lifted below for the present invention:
Embodiment 1:
Adopt the mixing in rat stomach of dynamic bionic Mus stomach as depicted-duodenum digestive system unit simulation raw rice grain, grinding and digestion process, by detecting the pH of gastric content, particle size distribution and digestion product maltose concentration, and compare with internal Mus experimental result alive, thus verifying the effectiveness of this device。Its specific operation process is as follows:
(1) according to apparatus of the present invention, apparatus of the present invention are assembled complete；
(2) adjust the room temperature in incubation chamber: open the heating lamp in incubation chamber and digital display temperature controller, when the temperature in incubation chamber maintains 37 DEG C, close heating lamp；
(3) fill gastric juice: take a gastric juice pipe, the full gastric juice of fill wherein, then one end of gastric juice pipe to be connected with single channel syringe pump A, the other end is connected with pyloric canal；
(4) foodstuff samples is prepared: weigh the grain of rice (d < 0.6mm:2.03g of 3.39g difference initial particle scope；0.60 < d < 0.85mm:0.66g；D > 0.85mm:0.70g) and 2.15g deionized water in a 15mL small beaker, be subsequently adding 1.15mL simulate rat saliva amylase solution (37 DEG C), magnetic agitation 1min (60rpm), to simulate the grain of rice at the intraoral mastication processes of rat；
(5) injecting gastric juice to bionic-mouse stomach model: inject 0.6mL simulated gastric fluid with syringe to bionic-mouse stomach model, during to simulate rat stomach fasting state, gastric juice remains；
(6) food is injected to bionic-mouse stomach model: be expelled to bionic-mouse stomach model by the food prepared in step (4) from esophagus is disposable, silica gel valve pipe is connected with esophagus outlet, prevent gastric food from overflowing, and block pyloric canal, to simulate the food digestion process when gastric does not empty；
(7) simulation food digestion process in bionic-mouse stomach model: initiation culture controller, electric compressor-rolling extrusion apparatus starts the glandular stomach of bionical rat stomach model is compressed vertically, simultaneously to glandular stomach from gastric antrum toward the rolling extrusion in pylorus direction, by single channel syringe pump A, gastric juice is pushed in bionic-mouse stomach model；
(8) time segment record digestion process: in step (6) described digestion process, digestion process is divided into four time periods, it is followed successively by and starts to digest 30min, 60min, 120min and 180min, each time period terminates rear stop frequency controller and single channel syringe pump A, take out bionic-mouse stomach model, and collect the digest in bionic-mouse stomach model, measure and record its pH, particle size distribution and maltose concentration respectively；
(9) clean bionic-mouse stomach model: after experiment terminates, clean bionic-mouse stomach model with deionized water in time。
Above-mentioned experimental result shows, the raw grain of rice is after digestion different time, and the pH of gastric digestion thing, particle size distribution are consistent with internal Mus experimental result alive with maltose concentration, all do not have significant difference (P > 0.05)。The experiment of internal Mus alive is compared with Vitro Experimental Results, and pH is gradually decrease to about 2.85 afterwards along with digestion time increase first rises。After digestion 180min, the grain of rice ratio of greater particle size (d > 0.85mm), all along with the increase of digestion time is gradually lowered, is reduced to 5% by initial 18%；Little particle diameter (d < 0.60mm) ratio gradually rises, and is risen to 80% by initial 62%；Medium grain size (0.60 < d < 0.85mm) ratio does not have significant change。
No matter at this dynamic bionic Mus peptic digestion system and device or live in Mus experiment in vivo, maltose concentration be all that serpentine is cumulative along with the increase of digestion time, the maltose concentration in 180min moment respectively 102mg.mL-1And 125mg.mL-1, but diversity not notable (P > 0.05)。And the relative increase of maltose concentration keeps highly consistent with both variation tendencies of digestion time。Additionally, the relative error of experimental data that experiment in vitro records is significantly lower than experiment in vivo data。
Embodiment 2:
Adopt the wheat starch granule that dynamic bionic Mus stomach as depicted-duodenum digestive system unit simulation contains 10% pectin in rat stomach and ID mixing, grinding, digestion and evacuation procedure, by detecting the pH of gastric content, maltose concentration, Starch Hydrolysis rate, gastric emptying rate, rheological property and microstructure, and compare with the experiment of internal Mus alive and pertinent literature result, thus verifying the effectiveness of this device further, and probe into the impact that water soluble dietary fiber pectin is digested at gastrointestinal tract on wheaten starch and empties。Its specific operation process is as follows:
(1) according to apparatus of the present invention, apparatus of the present invention are assembled complete；
(2) adjust the room temperature in incubation chamber: open the heating lamp in incubation chamber and digital display temperature controller, when the temperature in incubation chamber maintains 37 DEG C, close heating lamp；
(3) fill gastric juice, pancreatic juice and bile: take a gastric juice pipe, the full gastric juice of fill wherein, then one end of gastric juice pipe is connected with single channel syringe pump A, the other end is connected with pyloric canal, separately take a pancreatic juice pipe and a bile pipe, the full pancreatic juice of fill in pancreatic juice pipe, one end of pancreatic juice pipe is connected with single channel syringe pump B, the full bile of fill in bile pipe, one end of bile pipe is connected with single channel syringe pump C, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
(4) foodstuff samples is prepared:
Matched group: take 3.5g(dry weight) wheaten starch is placed in the container equipped with 6.5mL deionized water, after being sufficiently stirred for, configuration dry matter content is the solution of 35%, it is subsequently adding rat simulation saliva, then magnetic agitation 1 minute (60rpm), to simulate food at the intraoral digestion process of Mus；
Experimental group: take 3.5g(dry weight) wheaten starch containing 10% pectin powder is placed in the container equipped with 6.5mL deionized water, after being sufficiently stirred for, configuration dry matter content is the solution of 35%, it is subsequently adding rat simulation saliva, then magnetic agitation 1 minute (60rpm), to simulate food at the intraoral digestion process of Mus；
(5) injecting gastric juice to bionic-mouse stomach model: inject simulated gastric fluid with syringe to bionic-mouse stomach model, during to simulate rat stomach fasting state, gastric juice remains；
(6) foodstuff samples is injected to bionic-mouse stomach model: be expelled to bionic-mouse stomach model by the matched group prepared in step (4) or experimental group foodstuff samples from esophagus is disposable, silica gel valve pipe is connected with esophagus outlet, prevent gastric food from overflowing, and keep gastric voltage stabilizing；
(7) digestion process of simulation bionic-mouse stomach model and bionical duodenum model pipe: initiation culture controller, electric compressor-rolling extrusion apparatus starts the glandular stomach of bionical rat stomach model is compressed vertically, simultaneously to glandular stomach from gastric antrum toward the rolling extrusion in pylorus direction, by single channel syringe pump A, gastric juice is pushed in bionic-mouse stomach model, by single channel syringe pump B, pancreatic juice is pushed in bionical duodenum model pipe, by single channel syringe pump C, bile is pushed in bionical duodenum model pipe, pancreatic juice and bile and the chyme from the discharge of bionic-mouse stomach model are transported to bionical duodenum model pipe by peristaltic pump, bionical duodenum model pipe is carried out segmentation wriggling extruding by electric peristaltic extruding apparatus, chyme is discharged from bionical duodenum model pipe the most at last, it is collected bottle to collect；
(8) time segment record digestion process: in step (7) described digestion process, digestion process is divided into eight time periods, it is followed successively by and starts to digest 10, 20, 30, 40, 60, 90, 120 and 180min, a frequency controller is stopped in each time period, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, take out bionic-mouse stomach model and bionical duodenum model pipe, collect the digest in bionic-mouse stomach model and bionical duodenum model pipe respectively, measure its pH respectively, maltose concentration, Starch Hydrolysis rate, gastric emptying rate, rheological property (storage modulus, loss modulus, dynamic viscosity and apparent viscosity) and microstructure, after experiment terminates, close frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C；
(9) clean bionic-mouse stomach model and bionical duodenum model pipe: after experiment terminates, clean bionic-mouse stomach model and bionical duodenum model pipe in time with deionized water。
Above-mentioned experiment shows, the experimental group and matched group foodstuff samples its gastric digestion thing pH that add 10% Fructus Mangifera Indicae powder first raise along with the increase of digestion time and be gradually lowered afterwards, and duodenum internal diabetes compound pH remains at about 7。The maltose concentration of experimental group digest, Starch Hydrolysis rate and gastric emptying rate are all substantially less than matched group, but storage modulus, loss modulus, dynamic viscosity and apparent viscosity are significantly higher than matched group (P < 0.05)。Starch granules is wrapped up therein by microstructure observing result display pectin by forming gel networks, and prevention digestive enzyme effectively contacts with substrate, thus reducing Starch Hydrolysis rate。Above-mentioned experimental result can be passed through with the dietary fiber pectin that the experiment of internal Mus alive and pertinent literature result are reported to increase food viscosity and form gel networks parcel starch granules thus reducing the mixing of food, gastric emptying rate and starch digestibility to keep consistent。
Technological means disclosed in the present invention program is not limited only to the technological means disclosed in above-mentioned technological means, also includes the technical scheme being made up of above technical characteristic combination in any。
With the above-mentioned desirable embodiment according to the present invention for enlightenment, by above-mentioned description, relevant staff in the scope not necessarily departing from this invention technological thought, can carry out various change and amendment completely。The technical scope of this invention is not limited to the content in description, it is necessary to determine its technical scope according to right。
Claims (9)
1. bionical dynamic Mus stomach-duodenum digestive system analog, this analog is for simulating food digestion in duodenum then through pancreatic juice and bile after the stomach back warp of Mus crosses Gastric juice digestion, for studying in digestion process and the change of final chyme, it is characterized in that including peptic digestion system, duodenum digestive system, stream adds-emptier and incubation chamber, described peptic digestion system, duodenum digestive system and stream adds-and emptier is respectively positioned in incubation chamber, stream adds-and emptier carries gastric juice towards peptic digestion system, bile and pancreatic juice is carried towards duodenum digestive system, peptic digestion system and duodenum digestive system are interconnected；
Described peptic digestion system includes bionic-mouse stomach model and electric compressor-rolling extrusion apparatus, equipped with foodstuff samples in described bionic-mouse stomach model, described electric compressor-rolling extrusion apparatus is used for rolling extrusion bionic-mouse stomach model, and bionic-mouse stomach model produces simulation stomach and shrinks-roll digestion action；
Described duodenum digestive system includes bionical duodenum model pipe and electric peristaltic extruding apparatus, foodstuff samples in described bionic-mouse stomach model is discharged in bionical duodenum model pipe after bionic-mouse stomach model digests, described electric peristaltic extruding apparatus is used for the bionical duodenum model pipe of extruding of wriggling, and bionical duodenum model pipe produces the action of simulation duodenum wriggling digestion；
Described stream adds-and emptier includes single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, described single channel syringe pump A is by gastric juice pipe connection bionic-mouse stomach model, single channel syringe pump A carries gastric juice in bionic-mouse stomach model, described single channel syringe pump B connects bionical duodenum model pipe by pancreatic juice pipe, single channel syringe pump C connects bionical duodenum model pipe by bile pipe, single channel syringe pump B carries pancreatic juice in bionical duodenum model pipe, and single channel syringe pump C carries bile in bionical duodenum model pipe；
The free end of described bionical duodenum model pipe is provided with chyme receiving flask, and described chyme receiving flask is for collecting the chyme discharged from bionical duodenum model pipe。
2. bionical dynamic Mus stomach-duodenum digestive system analog according to claim 1, is characterized in that described bionic-mouse stomach model is utilize the Mus stomach that real full enlarged abdomen is little to make molding by overmolded, uses " DragonSkin?" addition-type silicon rubber is made, the size of described bionic-mouse stomach model, shape is consistent with true rat stomach with internal structure, with boundary ridge for boundary, it is divided into glandular stomach and glandular stomach, glandular stomach inner wall smooth, glandular stomach inwall is full of fold, glandular stomach connection esophagus, silica gel valve pipe is installed in esophagus, glandular stomach connection pyloric canal and gastric juice secretory duct, described gastric secretion pipe connection single channel syringe pump A, described pyloric canal connects bionical duodenum model pipe, food enters bionic-mouse stomach model from esophagus, it is expelled to bionical duodenum model pipe from pyloric canal after bionic-mouse stomach model digests。
3. bionical dynamic Mus stomach-duodenum digestive system analog according to claim 2, it is characterized in that described electric compressor-rolling extrusion apparatus includes motor, support, eccentric, frequency controller, gusset, belt, belt pulley, bevel gear, pressing plate, fixing plate, gripper shoe and base, described support is fixed on base, described support is provided with two fixing plates, it is fixed with gripper shoe between the fixing plate of said two, described bionic-mouse stomach model is installed on the supporting plate, each fixing plate is provided with a motor, bevel gear connecting eccentric wheel is passed through in one end of motor, glandular stomach is carried out compression vertical up and down with dynamic pressure plate by eccentric rotation process, described fixing plate is additionally provided with three belt pulleys of distribution triangular in shape, described belt pulley is cased with belt, one of them belt pulley has step motor drive, motor drives belt and then drives eccentric glandular stomach to be carried out from gastric antrum toward pylorus direction rolling extrusion, described frequency controller is connected with motor, frequency controller regulates bionic-mouse stomach model by the rotating speed of control motor and is subject to compression and rolling extrusion frequency and amplitude。
4. bionical dynamic Mus stomach-duodenum digestive system analog according to claim 1, it is characterized in that the length of described bionical duodenum model pipe and internal diameter are equal with the duodenal length of true Mus and internal diameter size, pyloric canal and bionical duodenum model pipe are connected by the four-way pipe of " K " word shape, one end of " I " part of the four-way pipe of described " K " word shape is connected with pyloric canal, the other end is connected with bionical duodenum model pipe, one end of " < " part of the four-way pipe of " K " word shape is connected with single channel syringe pump B by pancreatic juice pipe, the other end is connected with single channel syringe pump C by bile pipe。
5. bionical dynamic Mus stomach-duodenum digestive system analog according to claim 4, it is characterized in that described external electric wriggling extruding apparatus includes motor, eccentric, frequency controller, bevel gear, fast pulley, gripper shoe, fixing plate, belt, belt pulley and base, described support is fixed on base, fixing plate on described support, described fixing plate arranges several fast pulleys, described bionical duodenum model pipe is curved through each fast pulley successively, the side of described fixing plate is provided with two motors, described every motor is each through bevel gear connecting eccentric wheel, bionical duodenum model pipe is carried out the wriggling extruding of subsection interleaving formula by described eccentric, described frequency controller connects motor, frequency controller carrys out the eccentric adjustment wheel wriggling extruding frequency to bionical duodenum model pipe by controlling the rotating speed of motor。
6. bionical dynamic Mus stomach-duodenum digestive system analog according to claim 5, is characterized in that the chyme upstream end of described bionical duodenum model pipe is provided with peristaltic pump。
7. bionical dynamic Mus stomach-duodenum digestive system analog according to claim 1, it is characterized in that being provided with in described incubation chamber in heating heat-preserving box air heating lamp and for being illuminated illuminating lamp in incubation chamber, being additionally provided with the digital display temperature controller for monitoring the real time temperature within incubation chamber in described incubation chamber, the side of described incubation chamber is provided with sliding door。
8. the analogue experiment method of any of the above-described described bionical dynamic Mus stomach-duodenum digestive system analog of application, is characterized in that comprising the steps:
(1) adjusting the room temperature in incubation chamber: opening the heating lamp in incubation chamber and digital display temperature controller, the temperature in incubation chamber maintains 37 DEG C all the time, when temperature is higher than 37 DEG C, will turn off heating lamp, during lower than 37 DEG C, heating lamp then automatically turns on；
(2) fill gastric juice, pancreatic juice and bile: take a gastric juice pipe, the full gastric juice of fill wherein, then one end of gastric juice pipe is connected with single channel syringe pump A, the other end is connected with pyloric canal, separately take a pancreatic juice pipe and a bile pipe, the full pancreatic juice of fill in pancreatic juice pipe, one end of pancreatic juice pipe is connected with single channel syringe pump B, the full bile of fill in bile pipe, one end of bile pipe is connected with single channel syringe pump C, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
(3) foodstuff samples is prepared: take food and be placed in equipped with, in the container of deionized water, after being sufficiently stirred for, adding Mus saliva, then magnetic agitation, to simulate food at the intraoral digestion process of Mus；
(4) injecting gastric juice to bionic-mouse stomach model: inject simulated gastric fluid with syringe to bionic-mouse stomach model, during to simulate rat stomach fasting state, gastric juice remains；
(5) inject foodstuff samples to bionic-mouse stomach model: be expelled to bionic-mouse stomach model by the foodstuff samples prepared in step (3) from esophagus is disposable, silica gel valve pipe is connected with esophagus outlet, it is prevented that gastric food overflows, and keeps gastric voltage stabilizing；
(6) digestion process of simulation bionic-mouse stomach model and bionical duodenum model pipe: initiation culture controller, electric compressor-rolling extrusion apparatus starts the glandular stomach of bionical rat stomach model is compressed vertically, simultaneously to glandular stomach from gastric antrum toward the rolling extrusion in pylorus direction, by single channel syringe pump A, gastric juice is pushed in bionic-mouse stomach model, by single channel syringe pump B, pancreatic juice is pushed in bionical duodenum model pipe, by single channel syringe pump C, bile is pushed in bionical duodenum model pipe, pancreatic juice and bile and the chyme from the discharge of bionic-mouse stomach model are transported to bionical duodenum model pipe by peristaltic pump, bionical duodenum model pipe is carried out segmentation wriggling extruding by electric peristaltic extruding apparatus, chyme is discharged from bionical duodenum model pipe the most at last, it is collected bottle to collect；
(7) time segment record digestion process: in step (6) described digestion process, digestion process is divided into several time periods, stops a frequency controller in each time period, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, takes out bionic-mouse stomach model and bionical duodenum model pipe, collects the digest in bionic-mouse stomach model and bionical duodenum model pipe respectively, measures respectively and record its pH, maltose concentration, gastric emptying rate, storage modulus, loss modulus, dynamic viscosity and apparent viscosity, and observe microstructure, then chyme is put back to again in bionic-mouse stomach model and bionical duodenum model pipe, by bionic-mouse stomach model and bionical duodenum model pipe retrieval device, open frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, continues experiment, until chyme is all discharged from bionical duodenum model pipe, and measures and the final pH discharging chyme of record, maltose concentration, gastric emptying rate, storage modulus, loss modulus, dynamic viscosity and apparent viscosity, and observe microstructure；
(8) clean bionic-mouse stomach model and bionical duodenum model pipe: after experiment terminates, clean bionic-mouse stomach model and bionical duodenum model pipe in time with deionized water。
9. the analogue experiment method of the bionical dynamic Mus stomach-duodenum digestive system analog of application according to claim 8, is characterized in that in described step (7), microstructure is observed by laser confocal microscope CSLM and scanning electron microscope sem。
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