CN110839779A - Glucose oxidase composite product for reducing feed conversion ratio of broiler chickens and application thereof - Google Patents
Glucose oxidase composite product for reducing feed conversion ratio of broiler chickens and application thereof Download PDFInfo
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- CN110839779A CN110839779A CN201911285485.2A CN201911285485A CN110839779A CN 110839779 A CN110839779 A CN 110839779A CN 201911285485 A CN201911285485 A CN 201911285485A CN 110839779 A CN110839779 A CN 110839779A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/132—Heterocyclic compounds containing only one nitrogen as hetero atom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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Abstract
The invention relates to the technical field of enzymes, in particular to a glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens and application thereof. The glucose oxidase composite product is prepared from the following active ingredients in parts by weight: 5-35 parts of glucose oxidase, 1-7 parts of protease, 1.5-7.4 parts of lipase, 15-71 parts of tributyrin, 13-50 parts of betaine, 1-5 parts of biotin, 1-4 parts of inulin and 1-4 parts of ethoxyquinoline. The glucose oxidase composite product can provide a remarkable effect on reduction of the feed conversion ratio of the broiler chickens, reduce the occurrence rate of excrement and urine, reduce the cost and improve the economic benefit.
Description
Technical Field
The invention relates to the technical field of enzymes, in particular to a glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens and application thereof.
Background
Glucose oxidase as a green feed additive enters the feed additive catalog in 1999, but is not effectively popularized due to high production and use cost. In recent years, with the reduction of production cost, the cost performance of the glucose oxidase is higher and higher, and the glucose oxidase has good functions of inhibiting bacteria and improving intestinal micro-ecological environment, so as to reduce the identity of persons who resist to practice, and is gradually approved by feed factories and terminal farmers, and the application of the glucose oxidase in animal feed, particularly in poultry feed, is gradually increased.
The glucose oxidase is used as a beneficial component, and can play a certain role in improving the antibacterial effect and improving the intestinal micro-ecological environment when being singly added or being added with other components in a composite way. However, in the current whole animal breeding industry, various additives are various in variety and different in use mode, and the efficacy and the use range of the product are greatly exaggerated in propaganda and popularization no matter the additive is a single-component additive or a compound additive product, and the same problem also exists in the use of the glucose oxidase.
The invention aims to provide a composite product taking glucose oxidase as a main component, and the combination of the main component and the auxiliary component of the composite product has a better function of promoting the growth of broiler chickens compared with the existing single glucose oxidase product and the existing composite product, can improve the utilization rate of feed and reduce the feed conversion ratio of broiler chickens, thereby increasing the economic benefit of broiler chicken breeding and achieving the purpose of high-efficiency breeding.
Disclosure of Invention
The invention provides a glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens and application thereof, and solves the problems in the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens is prepared from the following active ingredients in parts by weight:
5-35 parts of glucose oxidase, 1-7 parts of protease, 1.5-7.4 parts of lipase, 15-71 parts of tributyrin, 13-50 parts of betaine, 1-5 parts of biotin, 1-4 parts of inulin and 1-4 parts of ethoxyquinoline.
Preferably, the glucose oxidase composite product for reducing the feed conversion ratio of the broiler chickens is prepared from the following active ingredients in parts by weight:
15 parts of glucose oxidase, 2-3.5 parts of protease, 1.5-2 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 1-2 parts of biotin, 1-2 parts of inulin and 2 parts of ethoxyquinoline
Preferably, the glucose oxidase composite product for reducing the feed conversion ratio of the broiler chickens is prepared from the following active ingredients in parts by weight:
15 parts of glucose oxidase, 3 parts of protease, 2 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 2 parts of biotin, 1 part of inulin and 2 parts of ethoxyquinoline.
Preferably, the enzyme activity of the glucose oxidase is 0.2 ten thousand U/g, the enzyme activity of the protease is 15-100 ten thousand U/g, and the enzyme activity of the lipase is 20 ten thousand U/g; the purity of tributyrin is 60%, the purity of betaine is 98%, the purity of biotin is 2%, the purity of inulin is 50%, and the purity of ethoxyquinoline is 4.5%.
Preferably, the protease is an acid protease, a neutral protease, an alkaline protease according to 4: 3: 2 by weight ratio.
Preferably, the alkaline protease is a keratinase.
The glucose oxidase composite product for reducing the feed conversion ratio of the broiler chickens is applied to preparation of livestock feed.
The addition amount of the glucose oxidase composite product for reducing the feed conversion ratio of the broiler chickens in the livestock feed is 1 kg/ton.
The livestock feed is broiler feed.
The invention has the beneficial effects that:
the glucose oxidase composite product for reducing the feed conversion ratio of the broiler chicken is added with protease, lipase, tributyrin, betaine, biotin, inulin, ethoxyquinoline and other components on the basis of the glucose oxidase, so that a remarkable effect can be provided for reducing the feed conversion ratio of the broiler chicken; the protease creatively adopts the acid protease, the neutral protease and the keratinase which are mixed according to a specific proportion, and compared with a single protease species, the effect of increasing the daily gain of the broiler chicken can be improved on the basis of better reducing the feed conversion ratio of the broiler chicken. In addition, the composite product firstly uses three products of glucose oxidase, betaine and tributyrin according to the ratio of 3: 10: 5, the feed-meat ratio of the broiler chickens is obviously reduced, the harm of the broiler chickens caused by heat stress in a hot environment can be prevented, the occurrence rate of the broiler chickens in water is reduced, and the economic benefit is improved.
Detailed Description
In order to clearly illustrate the technical features of the present invention, the present invention is explained in detail by the following embodiments.
Example 1
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 15 parts of glucose oxidase, 3 parts of protease, 2 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 2 parts of biotin, 1 part of inulin and 2 parts of ethoxyquinoline;
wherein the protease is selected from acid protease, neutral protease, and keratinase according to a ratio of 4: 3: 2 by weight ratio.
Example 2
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 15 parts of glucose oxidase, 3.5 parts of protease, 2 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 1 part of biotin, 1.5 parts of inulin and 2 parts of ethoxyquinoline;
wherein the protease is selected from acid protease, neutral protease, and keratinase according to a ratio of 4: 3: 2 by weight ratio.
Example 3
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 15 parts of glucose oxidase, 2.5 parts of protease, 1.5 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 2 parts of biotin, 2 parts of inulin and 2 parts of ethoxyquinoline;
wherein the protease is selected from acid protease, neutral protease, and keratinase according to a ratio of 4: 3: 2 by weight ratio.
Example 4
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 15 parts of glucose oxidase, 3 parts of protease, 2 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 2 parts of biotin, 1 part of inulin and 2 parts of ethoxyquinoline;
wherein the protease is selected from acid protease, neutral protease, and common alkaline protease according to a ratio of 4: 3: 2 by weight ratio.
Example 5
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 15 parts of glucose oxidase, 3 parts of protease, 2 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 2 parts of biotin, 1 part of inulin and 2 parts of ethoxyquinoline;
wherein the protease is selected from acid protease and neutral protease according to the ratio of 6: 3 by weight ratio.
This example removes alkaline protease without changing the total amount of protease used.
Example 6
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 30 parts of glucose oxidase, 6 parts of protease, 4 parts of lipase, 50 parts of betaine, 4 parts of biotin, 2 parts of inulin and 4 parts of ethoxyquinoline;
wherein the protease is selected from acid protease, neutral protease, and keratinase according to a ratio of 4: 3: 2 by weight ratio.
This example removes the compound tributyrin, with the total product amount unchanged.
Example 7
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 20 parts of glucose oxidase, 5 parts of protease, 4 parts of lipase, 66 parts of tributyrin, 3 parts of biotin and 2 parts of ethoxyquinoline;
wherein the protease is selected from acid protease, neutral protease, and keratinase according to a ratio of 4: 3: 2 by weight ratio.
This example removes the complex components betaine and inulin without changing the total product.
Example 8
A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens comprises the following raw materials in parts by weight: 18 parts of glucose oxidase, 3 parts of protease, 2 parts of lipase, 42 parts of tributyrin, 30 parts of betaine, 2 parts of biotin, 1 part of inulin and 2 parts of ethoxyquinoline;
wherein the protease is selected from acid protease, neutral protease, and keratinase according to a ratio of 4: 3: 2 by weight ratio.
This example adjusted the addition ratio of tributyrin, betaine, and glucose oxidase.
In each embodiment, the enzyme activity of the glucose oxidase is 0.2 ten thousand U/g, the enzyme activity of the protease is 80 ten thousand U/g, and the enzyme activity of the lipase is 20 ten thousand U/g; the purity of tributyrin is 60%, the purity of betaine is 98%, the purity of biotin is 2%, the purity of inulin is 50%, and the purity of ethoxyquinoline is 4.5%.
The test for reducing the growth feed-meat ratio of the broiler chicken comprises the following steps:
1. materials and methods
7.6 million ROSS-308 broilers with basically the same conditions of day age, weight, mental state and the like are selected for test chickens and divided into a control group and a test group, wherein each group is divided into two groups, and each group is divided into 1.9 thousands. 1 kg/ton of glucose oxidase complex product was added to the feed in the test group ration according to the level of each example, wherein tests 1 to 8 correspond to the glucose oxidase complex products of examples 1 to 8, respectively. The whole feeding process is added, the glucose oxidase compound product is not added to the control group, and feeding tests are carried out by adopting the same management mode (feeding daily ration, immune program, medicine health care and the like) under the condition that the chicken house environment (temperature, humidity, ventilation and the like) is basically the same. And in the test period of 42 days, the mortality, the feed consumption, the slaughter weight and other data of the chickens are recorded in the test process, and the results are analyzed according to the recorded test data after the test is finished.
2. And (3) test results: the concrete results are as follows
As can be seen from Table 1, the average mortality and mortality of the test groups (examples 1 to 8) was 0.3% lower than that of the control group, and the average feed-meat ratio was 0.05% lower than that of the control group; under the condition that the addition amounts and the addition ratios of three main components of the tributyrin, the betaine and the glucose oxidase are fixed, the reduction degree of the feed-meat ratio of the broiler chicken is maximum and reaches 0.08. As can be seen from Table 2, the economic benefit of the test groups (examples 1-8) is on average 0.62 ten thousand yuan higher than that of the control group, and the average gain of each chicken is 0.33 yuan higher than that of the control group; wherein the economic benefit of the test groups 1-3 is 1.22 ten thousand yuan higher than that of the control group on average, and the average benefit of each chicken is 0.64 yuan higher than that of the control group.
TABLE 1 influence of glucose oxidase Complex products on broiler Productivity
As can be seen from the data in table 1, compared with the feed without the glucose oxidase compound product, the feed with the glucose oxidase compound product has an obvious improvement effect on each production performance index in broiler breeding, and when the formula components and the dosage of the oxidase compound product are changed, each production performance index also has certain fluctuation and influence. Of these, examples 1 to 3 are preferred. After the scheme of example 4 changes the alkaline protease species, the feed-meat ratio improvement degree is obviously reduced, and the feed consumption is improved. The improvement of the feed-meat ratio is reduced after the protease species are reduced by the scheme of example 5. In examples 6 and 7, after one or two components in the scheme are respectively removed, the feed conversion ratio is obviously reduced. After the scheme of the example 8 adjusts the proportion of the glucose oxidase, the tributyrin and the betaine, the improvement degree of the feed-meat ratio is obviously reduced.
TABLE 2 analysis of economic benefits of glucose oxidase complex products
Note: the unit price of the chicks is 0.9 yuan per chicken, the number of the chicks is 19000, the unit price of the broilers is 7.8 yuan per kg, and the unit price of the feed is 2900 yuan per ton.
The above-described embodiments should not be construed as limiting the scope of the invention, and any alternative modifications or alterations to the embodiments of the present invention will be apparent to those skilled in the art.
The present invention is not described in detail, but is known to those skilled in the art.
Claims (8)
1. A glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens is characterized by being prepared from the following active ingredients in parts by weight:
5-35 parts of glucose oxidase, 1-7 parts of protease, 1.5-7.4 parts of lipase, 15-71 parts of tributyrin, 13-50 parts of betaine, 1-5 parts of biotin, 1-4 parts of inulin and 1-4 parts of ethoxyquinoline.
2. The glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens of claim 1, is characterized by being prepared from the following active ingredients in parts by weight:
15 parts of glucose oxidase, 2-3.5 parts of protease, 1.5-2 parts of lipase, 50 parts of tributyrin, 25 parts of betaine, 1-2 parts of biotin, 1-2 parts of inulin and 2 parts of ethoxyquinoline.
3. The glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens of claim 1, wherein the enzyme activity of glucose oxidase is 0.2 ten thousand U/g, the enzyme activity of protease is 15-100 ten thousand U/g, and the enzyme activity of lipase is 20 ten thousand U/g; the purity of tributyrin is 60%, the purity of betaine is 98%, the purity of biotin is 2%, the purity of inulin is 50%, and the purity of ethoxyquinoline is 4.5%.
4. The glucose oxidase composite product for reducing the feed conversion ratio of broiler chickens of claim 1, wherein the protease is acid protease, neutral protease, keratinase according to a ratio of 4: 3: 2 by weight ratio.
5. The glucose oxidase composite product for reducing feed conversion ratio of broiler chickens of claim 4, wherein said keratinase is keratinase.
6. Use of the glucose oxidase complex product for reducing feed conversion ratio of broiler chickens of any one of claims 1-5 in the preparation of livestock feed.
7. The use of claim 6, wherein the glucose oxidase complex product for reducing the feed conversion ratio of broiler chickens is added in the livestock feed in an amount of 1 kg/ton.
8. Use according to claim 7, wherein the livestock feed is a broiler feed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911285485.2A CN110839779A (en) | 2019-12-13 | 2019-12-13 | Glucose oxidase composite product for reducing feed conversion ratio of broiler chickens and application thereof |
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| CN201911285485.2A CN110839779A (en) | 2019-12-13 | 2019-12-13 | Glucose oxidase composite product for reducing feed conversion ratio of broiler chickens and application thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417919A (en) * | 2016-10-14 | 2017-02-22 | 山东德州神牛药业有限公司 | Special intestinal balance regulator for livestock as well as preparation method and application thereof |
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- 2019-12-13 CN CN201911285485.2A patent/CN110839779A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417919A (en) * | 2016-10-14 | 2017-02-22 | 山东德州神牛药业有限公司 | Special intestinal balance regulator for livestock as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈桂林: "《中国饲料原料采购指南》", 31 July 2002, 企业管理出版社 * |
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Application publication date: 20200228 |