CN110804192A - 一种纤维素抗菌水凝胶及其制备方法 - Google Patents
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Abstract
本发明公开了一种新型的生物相容性的抗菌细菌纤维素Fmoc‑F水凝胶的制备方法,属于生物医学凝胶技术领域,其特征是包括如下步骤:(1)细菌纤维素(BC)匀浆的制备(2)将细菌纤维素与Fmoc‑F溶液混合,采用原位化学交联的手段制备细菌纤维素/Fmoc‑F抗菌水凝胶。该方法具有成胶反应迅速、反应体系简单以及凝胶可塑性高等优点。由于细菌纤维素的存在,本发明制备的抗菌水凝胶的机械强度高。与传统的小分子抗菌材料相比,本发明制备的细菌纤维素/Fmoc‑F抗菌水凝胶具有广谱的抑菌效果并且生物相容性良好。
Description
技术领域
本发明涉及生物医学凝胶技术领域,特别涉及一种生物相容性的抗菌细菌纤维素/Fmoc-L-苯丙氨酸水凝胶的制备方法。
背景技术
现如今,虽然人类在医疗健康领域取得了很大的进步,但是由病毒、细菌和真菌等病原体引起的传染病仍然是一种主要的健康威胁,并且可以转化为广泛的社会经济问题。而随着社会经济的发展,人们对于生活质量和健康品质的追求也不断提升,抗菌产品被人们认为是具有广阔发展前景的新型健康产品之一。传统的抗菌材料是通过化学或者物理的方法在材料表面或本体引入一些抗菌剂(如抗生素、季铵盐和杀菌剂等)来赋予这些生物医用材料一定的抗菌能力。但是由于抗生素类的小分子抗菌剂通常具有特异性并且容易导致细菌产生抗药性。因此,开发新型的具有广谱抗菌能力的抗菌材料就显得非常的迫切。
水凝胶是由亲水聚合物通过物理或化学交联形成的具有三维(3D)网状结构的新型高分子功能材料,能够在聚合物网络中保留大量的水。由于水凝胶具有优异的生物相容性,因此被广泛应用于药物输送、组织工程、伤口愈合等各种生物医用材料领域中。抗菌水凝胶不仅具有多种聚合物的特性,良好的相容性,而且可以有效的抗击细菌感染,抑制细菌耐药性的产生,促进伤口愈合。因此,近年来,抗菌水凝胶成为一种新型的伤口敷料应用于医疗领域。
生物聚合物在水凝胶生产中引起了人们的注意,它是一种具有生物相容性和生态环境友好的可再生资源。细菌纤维素(BC)是一种由细菌产生的天然生物聚合物。BC具有一些独特的性质,如生物活性、生物可降解性、生物适应性、无毒、无过敏反应、良好的机械韧性、优良的保水性、较高的纯度、较高的孔隙度和较高的结晶度等,使它成为生物医用水凝胶的重要原材料。细菌纤维素基水凝胶可以有效缓解疼痛,有良好的附着性,,有效防止细菌的入侵感染,有利于皮肤组织生长,快速促进伤口的快速愈合。
Fmoc-L-苯丙氨酸(Fmoc-F)为Fmoc保护的苯丙氨酸,主要用于合成多肽,氨基酸保护基的不同对多肽合成的效率和产率有很大的影响。Fmoc-苯丙氨酸可以用于制备一种苯丙氨酸抑菌剂,从而抑制细菌生长。将Fmoc-L-苯丙氨酸和细菌纤维素溶解在PB溶液中(PH=7.4),在80℃下加热30min后得到的水凝胶兼具了两种材料的优点,具有良好的生物相容性、机械性能和抑菌性能,可以将其广泛应用于生物医用材料领域。
发明内容
本发明的目的在于提供一种新型的生物相容性优良的抗菌细菌纤维素/Fmoc-L-苯丙氨酸水凝胶及其制备的方法。该水凝胶具有良好的抗菌活性,抗菌活性持久。
本发明的技术原理:
以生物相容性良好的细菌纤维素生物聚合物和Fmoc-L-苯丙氨酸为主要材料,采用原位化学交联的手段制备细菌纤维素/Fmoc-L-苯丙氨酸抗菌水凝胶。
为了实现上述目的,解决上述技术问题,本发明采用的技术方案为:
(1)细菌纤维素匀浆的制备
利用组织匀浆机通过机械匀浆法破碎细菌纤维素膜,得到细菌纤维素匀浆。得到的匀浆以10,000rpm/min的转速离心10分钟,以去除多余的水分,得到细菌纤维素匀浆,浓度为10mg/ml(干重)。
(2)细菌纤维素/Fmoc-L-苯丙氨酸水凝胶的制备
室温下,用分析天平精确称量120mg Fmoc-F于50ml离心管中,添加4mlPB溶液溶解,每个离心管分别添加4ml,8ml,12ml,16ml BC匀浆,用PB溶液补齐到20ml。并蜗旋震荡1min,然后放入80℃的水浴锅中加热30min,之后室温放置6h后,即得到BC-Fomc-F水凝胶。即得到Fmoc-F浓度为6mg/ml,BC浓度依次为2mg/ml,4mg/ml,6mg/ml,8mg/ml。
而且,所述细菌纤维素悬浮液的浓度为10mg/ml。
而且,所述细菌纤维素悬浮液中纤维平均长度为10-100微米。
本发明的有益效果:
(1)本发明制备的BC-Fomc-F抗菌水凝胶对金黄色葡萄球菌和枯草芽孢杆菌均具有良好的抑菌效果。
(2)与传统的小分子抗菌材料相比,本发明提供了一种更广谱的抗菌复合材料的制备方法。
(3)本发明制备的抗菌水凝胶具有良好的生物相容性,生物可降解性无毒,并且机械强度高。
附图说明
图1为本发明制备的BC-Fomc-F抗菌水凝胶的效果图。
图2为本发明BC-Fomc-F抗菌水凝胶对枯草芽孢杆菌和金黄色葡萄球菌的抑菌圈实验效果图。(BC-0,BC-2,BC-4,BC-6,BC-8,分别代表BC的终浓度分别为0mg/ml,2mg/ml,4mg/ml,6mg/ml,8mg/ml。)
图3为本发明流变仪测出BC-Fomc-F抗菌水凝胶的储能模量(G’)和损耗模量(G”)随频率的变化曲线图。(BC-0,BC-2,BC-4,BC-6,BC-8代表的储能模量(G’)和损耗模量(G”)随频率的变化曲线图。)
具体实施方式
为了更好地理解本发明,下面结合实施例进一步阐明本发明的内容,但本发明的内容不仅仅局限于下面的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下获得的所有其他实施例,都属于本发明保护的范围。
一种抗菌BC-Fomc-F水凝胶的制备方法,步骤如下:
(1)细菌纤维素匀浆的制备,利用机械匀浆法将细菌纤维素膜破碎,得到细菌纤维素匀浆。之后将得到的匀浆高速离心10分钟,以去除多余的水分,得到细菌纤维素匀浆,浓度为10mg/ml(干重)。
(2)BC-Fomc-F水凝胶的制备,室温下,用分析天平精确称量120mg Fmoc-F于50ml离心管中,添加4ml PB溶液溶解,每个离心管分别添加4ml,8ml,12ml,16ml BC匀浆,用PB溶液补齐到20ml。并蜗旋震荡1min,然后放入80℃的水浴锅中加热30min,之后室温放置6h后,即得到BC-Fomc-F水凝胶。即得到Fmoc-F浓度为6mg/ml,BC浓度依次为2mg/ml,4mg/ml,6mg/ml,8mg/ml。
实施例1
利用组织匀浆机通过机械匀浆法破碎细菌纤维素膜,得到细菌纤维素匀浆。得到的匀浆以10,000rpm/min的转速离心10分钟,以去除多余的水分,得到细菌纤维素匀浆,浓度为10mg/ml(干重)。
室温下,用分析天平精确称量120mg Fmoc-F于50ml离心管中,添加4mlPB溶液溶解,离心管添加4ml BC匀浆,用PB溶液补齐到20ml。并蜗旋震荡1min,然后放入80℃的水浴锅中加热30min,之后室温放置6h后,即得到Fmoc-F浓度为6mg/ml,BC浓度为2mg/ml的BC-Fomc-F水凝胶。
实施例2
利用组织匀浆机通过机械匀浆法破碎细菌纤维素膜,得到细菌纤维素匀浆。得到的匀浆以10,000rpm/min的转速离心10分钟,以去除多余的水分,得到细菌纤维素匀浆,浓度为10mg/ml(干重)。
室温下,用分析天平精确称量120mg Fmoc-F于50ml离心管中,添加4mlPB溶液溶解,离心管添加8ml BC匀浆,用PB溶液补齐到20ml。并蜗旋震荡1min,然后放入80℃的水浴锅中加热30min,之后室温放置6h后,即得到Fmoc-F浓度为6mg/ml,BC浓度为4mg/ml的BC-Fomc-F水凝胶。
实施例3
利用组织匀浆机通过机械匀浆法破碎细菌纤维素膜,得到细菌纤维素匀浆。得到的匀浆以10,000rpm/min的转速离心10分钟,以去除多余的水分,得到细菌纤维素匀浆,浓度为10mg/ml(干重)。
室温下,用分析天平精确称量120mg Fmoc-F于50ml离心管中,添加4mlPB溶液溶解,离心管添加12ml BC匀浆,用PB溶液补齐到20ml。并蜗旋震荡1min,然后放入80℃的水浴锅中加热30min,之后室温放置6h后,即得到Fmoc-F浓度为6mg/ml,BC浓度为6mg/ml的BC-Fomc-F水凝胶。
实施例4
利用组织匀浆机通过机械匀浆法破碎细菌纤维素膜,得到细菌纤维素匀浆。得到的匀浆以10,000rpm/min的转速离心10分钟,以去除多余的水分,得到细菌纤维素匀浆,浓度为10mg/ml(干重)。
室温下,用分析天平精确称量120mg Fmoc-F于50ml离心管中,添加4ml PB溶液溶解,离心管添加16ml BC匀浆。并蜗旋震荡1min,然后放入80℃的水浴锅中加热30min,之后室温放置6h后,即得到Fmoc-F浓度为6mg/ml,BC浓度为8mg/ml的BC-Fomc-F水凝胶。
Claims (4)
1.一种抗菌BC-Fmoc-F水凝胶及其制备方法,其特征在于,包括以下步骤:
(1)细菌纤维素匀浆的制备:利用组织匀浆机通过机械匀浆法破碎细菌纤维素膜,得到细菌纤维素匀浆。得到的匀浆以10000rpm/min的转速离心10min,以去除多余的水分,得到细菌纤维素匀浆,浓度为10mg/ml(干重);
(2)细菌纤维素/Fmoc-L-苯丙氨酸水凝胶的制备:室温下,用分析天平精确称量120mgFmoc-F于50ml离心管中,添加4ml PB溶液溶解,每个离心管分别添加4ml,8ml,12ml,16mlBC匀浆,用PB溶液补齐到20ml。并蜗旋震荡1min,然后放入80℃的水浴锅中加热30min,之后室温放置6h后,即得到BC-Fomc-F水凝胶。即得到Fmoc-F浓度为6mg/ml,BC浓度依次为2mg/ml,4mg/ml,6mg/ml,8mg/ml。
2.根据权利要求1所述细菌纤维素匀浆的制备方法,其特征在于:所述细菌纤维素悬浮液的浓度为10mg/ml。
3.根据权利要求1所述细菌纤维素匀浆的制备方法,其特征在于:所述细菌纤维素悬浮液中纤维平均长度为10-100微米。
4.根据权利要求1所述BC-Fmoc-F水凝胶的制备方法,其特征在于:所述BC溶液的浓度分别为2mg/ml,4mg/ml,6mg/ml,8mg/ml。
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