CN110786236A - Method for improving drought resistance of chrysanthemum by PEG (polyethylene glycol) in-vitro culture - Google Patents
Method for improving drought resistance of chrysanthemum by PEG (polyethylene glycol) in-vitro culture Download PDFInfo
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- CN110786236A CN110786236A CN201910405498.2A CN201910405498A CN110786236A CN 110786236 A CN110786236 A CN 110786236A CN 201910405498 A CN201910405498 A CN 201910405498A CN 110786236 A CN110786236 A CN 110786236A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses a method for improving chrysanthemum drought resistance by PEG in-vitro culture, which comprises the following steps: taking the young sprout of chrysanthemum as an explant for in vitro culture, sterilizing, inoculating the young sprout of chrysanthemum into a rooting culture medium 1/2MS +20g/L sucrose +5g/L agar +1.0mg/L IBA + 10.0% PEG-6000, inducing to root, screening out bottle seedlings for rooting, further cutting off the root system, inoculating the young sprout of chrysanthemum into the rooting culture medium 1/2MS +20g/L sucrose +5g/L agar +1.0mg/L IBA + 10.0% PEG-6000, inducing to root, and repeating for 3 times. The bottle seedlings which survive are domesticated and transplanted into a greenhouse for conventional cultivation management, and chrysanthemum plants with high drought resistance are obtained.
Description
Technical Field
The invention relates to a method for improving drought resistance of Chrysanthemum (Chrysanthemum morifolium Rmat.) by PEG (polyethylene glycol) isolated culture, belonging to the field of cell biology and plants.
Background
With global warming, environmental deterioration and frequent drought, drought stress becomes one of the bottlenecks limiting chrysanthemum production and application. Therefore, the breeding of the new chrysanthemum variety with stronger drought resistance has important significance.
The chrysanthemum has bright color and rich flower types, and is deeply loved by people. At present, more than 3000 chrysanthemum varieties exist in China, and the cultivation area reaches 8900 hectare. The chrysanthemum is mainly used for landscaping, ground cover, interior decoration, tea, medicine and the like. Chrysanthemum belongs to a herbaceous plant with stronger drought resistance, but can grow badly in water-deficient areas, thereby influencing the sustainable development of the chrysanthemum industry. Therefore, the improvement of the drought resistance of the chrysanthemum is very important. In the aspect of chrysanthemum drought resistance research, researchers carry out a great deal of research work. The existing research shows that in the case of the American iso-transgenic DREB1A gene ground-cover chrysanthemum seedling as the experimental material, the potted seedling is naturally water-loss stressed under different temperature conditions, and the water loss condition of the transgenic ground-cover chrysanthemum seedling under the two environmental stresses of air and water drought is analyzed, and the results show that 5 transgenic chrysanthemum strains have better drought tolerance and water conservation than the wild type plants. The DREB gene is transferred into ground-cover chrysanthemum 'rain flower exciting chapter' by the Zuixinli et al, and the drought tolerance of the obtained transgenic line is detected, so that the drought tolerance of the transgenic line is improved to different degrees. Research on chrysanthemum varieties 'medal of flowers' of CgDREBa genes, such as Chensu plum and the like, shows that the transgenic chrysanthemum has higher survival rate and fresh weight preservation rate under drought stress compared with wild species. Also, studies show that drought stress affects the normal growth and development of chrysanthemum by inhibiting the photosynthesis of chrysanthemum, thereby affecting the yield and quality of chrysanthemum. However, there has been no report on obtaining chrysanthemum plants with strong drought resistance by in vitro culture using PEG. Therefore, the chrysanthemum bottle seedlings with strong tolerance and survival are screened in a mode of adding PEG into a culture medium in the process of chrysanthemum in vitro culture, and then chrysanthemum plants with strong drought resistance are obtained through domestication and screening.
Disclosure of Invention
The invention aims to obtain a method for improving the drought resistance of chrysanthemum by using PEG in-vitro culture so as to solve the problems.
The problem solved by the invention can be solved by adopting the following scheme:
a method for improving the drought resistance of chrysanthemum by PEG in-vitro culture is characterized by comprising the following steps:
taking the young sprout of chrysanthemum as an explant for in vitro culture, sterilizing, inoculating the young sprout of chrysanthemum into a rooting culture medium 1/2MS +20g/L sucrose +5g/L agar +1.0mg/L IBA + 10.0% PEG-6000, inducing to root, screening out bottle seedlings for rooting, further cutting off the root system, inoculating the young sprout of chrysanthemum into the rooting culture medium 1/2MS +20g/L sucrose +5g/L agar +1.0mg/L IBA + 10.0% PEG-6000, inducing to root, and repeating for 3 times. The bottle seedlings which survive are domesticated and transplanted into a greenhouse for conventional cultivation management, and chrysanthemum plants with high drought resistance are obtained.
Has the advantages that: by adopting the method, the drought resistance of the whole chrysanthemum plant is obviously improved. Can still normally grow under the drought condition that the water content of the soil reaches 18 percent. The invention obtains an effective method for improving the drought resistance of chrysanthemum by using PEG in-vitro culture, and obtains chrysanthemum plants with stronger drought resistance and longer ornamental period.
Drawings
FIG. 1 the chrysanthemum explants with buds are inoculated on the rooting medium containing PEG
FIG. 2 Chrysanthemum explant with buds cultured on rooting medium containing PEG for rooting
FIG. 3 Chrysanthemum plant suffering from drought stress (control)
FIG. 4 Normal growth of Chrysanthemum plants under drought stress (treatment)
Detailed Description
The present invention will be further described below in order to make the means, features, objectives and functions of the present invention easy to understand.
The bottle seedlings which survive are domesticated and transplanted into a greenhouse for conventional cultivation management, and chrysanthemum plants with high drought resistance are obtained.
After 3 years of continuous observation of the growth condition of chrysanthemum plants, the plants can grow normally under the drought condition that the soil moisture content reaches 18%, and the phenomenon of drought and wilting does not occur.
The invention obtains a method for improving the drought resistance of chrysanthemum by PEG in vitro culture.
Claims (1)
1. A method for improving drought resistance of chrysanthemum by PEG in-vitro culture comprises the following steps: taking the young sprout of chrysanthemum as an explant for in vitro culture, sterilizing, inoculating the young sprout of chrysanthemum into a rooting culture medium 1/2MS +20g/L sucrose +5g/L agar +1.0mg/LIBA + 10.0% PEG-6000, inducing to root, screening out bottle seedlings for root, further cutting off the root system, inoculating the young sprout of chrysanthemum into the rooting culture medium 1/2MS +20g/L sucrose +5g/L agar +1.0mg/L IBA + 10.0% PEG-6000, inducing to root, and repeating for 3 times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910405498.2A CN110786236A (en) | 2019-05-09 | 2019-05-09 | Method for improving drought resistance of chrysanthemum by PEG (polyethylene glycol) in-vitro culture |
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| CN201910405498.2A CN110786236A (en) | 2019-05-09 | 2019-05-09 | Method for improving drought resistance of chrysanthemum by PEG (polyethylene glycol) in-vitro culture |
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| CN110786236A true CN110786236A (en) | 2020-02-14 |
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| CN201910405498.2A Pending CN110786236A (en) | 2019-05-09 | 2019-05-09 | Method for improving drought resistance of chrysanthemum by PEG (polyethylene glycol) in-vitro culture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112586302A (en) * | 2021-01-26 | 2021-04-02 | 淮北师范大学 | Method for improving drought resistance of chrysanthemum |
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| CN104255449A (en) * | 2014-08-29 | 2015-01-07 | 江苏徐淮地区徐州农业科学研究所 | Indoor auxiliary sweet potato drought tolerance identification method |
| CN105660404A (en) * | 2016-02-05 | 2016-06-15 | 齐齐哈尔大学 | Method for PEG-resisting alfalfa callus dedifferentiation |
| CN105706923A (en) * | 2016-01-29 | 2016-06-29 | 广西壮族自治区亚热带作物研究所 | Method for screening drought-resistant variant sugarcane plants |
| CN106538388A (en) * | 2016-11-07 | 2017-03-29 | 中国农业科学院郑州果树研究所 | The authentication method of drought-enduring Actinidia under a kind of conditions of tissue culture |
| CN109169263A (en) * | 2018-10-22 | 2019-01-11 | 陈丁龙 | A kind of breeding method of drought resisting potato |
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2019
- 2019-05-09 CN CN201910405498.2A patent/CN110786236A/en active Pending
Patent Citations (5)
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| CN104255449A (en) * | 2014-08-29 | 2015-01-07 | 江苏徐淮地区徐州农业科学研究所 | Indoor auxiliary sweet potato drought tolerance identification method |
| CN105706923A (en) * | 2016-01-29 | 2016-06-29 | 广西壮族自治区亚热带作物研究所 | Method for screening drought-resistant variant sugarcane plants |
| CN105660404A (en) * | 2016-02-05 | 2016-06-15 | 齐齐哈尔大学 | Method for PEG-resisting alfalfa callus dedifferentiation |
| CN106538388A (en) * | 2016-11-07 | 2017-03-29 | 中国农业科学院郑州果树研究所 | The authentication method of drought-enduring Actinidia under a kind of conditions of tissue culture |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112586302A (en) * | 2021-01-26 | 2021-04-02 | 淮北师范大学 | Method for improving drought resistance of chrysanthemum |
| CN112586302B (en) * | 2021-01-26 | 2024-03-08 | 淮北师范大学 | Method for improving drought resistance of chrysanthemum |
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