CN110785654A - 系统级校准 - Google Patents

系统级校准 Download PDF

Info

Publication number
CN110785654A
CN110785654A CN201880040173.2A CN201880040173A CN110785654A CN 110785654 A CN110785654 A CN 110785654A CN 201880040173 A CN201880040173 A CN 201880040173A CN 110785654 A CN110785654 A CN 110785654A
Authority
CN
China
Prior art keywords
image
channel
calibration
intensity
channels
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880040173.2A
Other languages
English (en)
Chinese (zh)
Inventor
K·E·加尔沙
D·K·哈什曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of CN110785654A publication Critical patent/CN110785654A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/90—Dynamic range modification of images or parts thereof
    • G06T5/92—Dynamic range modification of images or parts thereof based on global image properties
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/70—Circuitry for compensating brightness variation in the scene
    • H04N23/73—Circuitry for compensating brightness variation in the scene by influencing the exposure time
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10144—Varying exposure

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Mathematical Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Signal Processing (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Microscoopes, Condenser (AREA)
CN201880040173.2A 2017-06-28 2018-06-26 系统级校准 Pending CN110785654A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762525818P 2017-06-28 2017-06-28
US62/525,818 2017-06-28
PCT/EP2018/067095 WO2019002281A1 (en) 2017-06-28 2018-06-26 CALIBRATION OF SYSTEM LEVEL

Publications (1)

Publication Number Publication Date
CN110785654A true CN110785654A (zh) 2020-02-11

Family

ID=62815014

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880040173.2A Pending CN110785654A (zh) 2017-06-28 2018-06-26 系统级校准

Country Status (5)

Country Link
US (2) US11747280B2 (enExample)
EP (2) EP4488657A1 (enExample)
JP (3) JP7068354B2 (enExample)
CN (1) CN110785654A (enExample)
WO (1) WO2019002281A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119151830A (zh) * 2024-08-15 2024-12-17 华南师范大学 一种用于定量荧光共振能量转移显微成像的照明光场校正方法及系统

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019002281A1 (en) * 2017-06-28 2019-01-03 Ventana Medical Systems, Inc. CALIBRATION OF SYSTEM LEVEL
EP3644044B1 (en) * 2018-10-24 2020-12-23 Leica Biosystems Imaging, Inc. Camera exposure control when acquiring fluorescence in situ hybridization images
US10890838B2 (en) * 2018-12-04 2021-01-12 University Of Massachusetts System and methods of fluorescence microscope calibration
US10895537B2 (en) 2019-04-11 2021-01-19 Perkinelmer Health Sciences, Inc. Laser intensity calibration
EP3961194B1 (en) * 2020-08-25 2023-11-08 Korea Advanced Institute of Science and Technology Method and apparatus for multiplexed imaging of biomolecules through iterative unmixing of fluorophore signals
US20220252514A1 (en) * 2021-02-10 2022-08-11 Star Voltaic, LLC Fluorescent solid-state materials for optical calibration and methods thereof
EP4290220B1 (en) * 2022-06-10 2025-08-27 Leica Microsystems CMS GmbH Controller for an imaging device and method
TWI870685B (zh) * 2022-07-27 2025-01-21 泓偊科技股份有限公司 對光源自動校正之自動光學檢查設備
AU2023353465A1 (en) * 2022-09-29 2024-10-03 Illumina, Inc. Dynamic optical system calibration
US12062204B2 (en) 2022-12-16 2024-08-13 Zomedica Inc. Methods and systems for slide imaging calibration
US20250132018A1 (en) * 2023-10-23 2025-04-24 Pramana, Inc. System and method of digitalizing a slide
WO2025115679A1 (ja) * 2023-12-01 2025-06-05 株式会社エビデント 光学顕微鏡及び性能判定方法
CN117764940B (zh) * 2023-12-19 2024-11-05 珠海圣美生物诊断技术有限公司 一种显微镜状态检测方法、装置、计算机设备及存储介质

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101368908A (zh) * 2007-08-17 2009-02-18 泰肯贸易股份公司 激光扫描装置
CN102165489A (zh) * 2008-09-16 2011-08-24 赫斯托克斯公司 生物标志物表达的可再现量化
WO2014118326A2 (en) * 2013-01-31 2014-08-07 Ventana Medical Systems, Inc. Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5485527A (en) * 1985-11-04 1996-01-16 Becton Dickinson And Company Apparatus and method for analyses of biological specimens
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US5880473A (en) * 1997-07-28 1999-03-09 Applied Imaging, Inc. Multifluor-fluorescence in-situ hybridization (M-FISH) imaging techniques using multiple multiband filters with image registration
AU763354B2 (en) 1998-02-27 2003-07-17 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
DE10033269B4 (de) * 2000-07-10 2010-07-01 Leica Microsystems Cms Gmbh Vorrichtung zum Einkoppeln von Licht mindestens einer Wellenlänge einer Laserlichtquelle in ein konfokales Rastermikroskop
JP3903000B2 (ja) * 2002-11-14 2007-04-11 アークレイ株式会社 測定装置、蛍光測定装置及び蛍光測定方法
US6897954B2 (en) * 2002-12-20 2005-05-24 Becton, Dickinson And Company Instrument setup system for a fluorescence analyzer
US8712118B2 (en) * 2003-04-10 2014-04-29 Carl Zeiss Microimaging Gmbh Automated measurement of concentration and/or amount in a biological sample
DE102005022880B4 (de) * 2005-05-18 2010-12-30 Olympus Soft Imaging Solutions Gmbh Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern
JP2007059179A (ja) * 2005-08-24 2007-03-08 Mitsutoyo Corp 照明装置の校正方法，校正装置，校正プログラム，記録媒体，照明装置，画像処理装置
JP4792880B2 (ja) * 2005-09-08 2011-10-12 パナソニック株式会社 微生物計数方法および微生物計数装置
EP1988383B1 (en) * 2006-02-23 2020-09-30 Nikon Corporation Spectrum image processing method, computer-executable spectrum image processing program, and spectrum imaging system
US7907271B2 (en) * 2007-06-15 2011-03-15 Historx, Inc. Method and system for standardizing microscope instruments
US20100033518A1 (en) * 2008-08-05 2010-02-11 Ali Shekarchi Lightboard
US8743195B2 (en) * 2008-10-24 2014-06-03 Leica Biosystems Imaging, Inc. Whole slide fluorescence scanner
JP2011085587A (ja) * 2009-10-15 2011-04-28 Becton Dickinson & Co 蛍光分析装置のための機器セットアップシステム
EP2993478B1 (en) 2009-10-19 2022-08-24 Ventana Medical Systems, Inc. Device and method for slide caching
JP4965706B2 (ja) * 2010-02-26 2012-07-04 Ｉｄｅｃ株式会社 蛍光スペクトルの識別方法
JP5655434B2 (ja) * 2010-08-30 2015-01-21 株式会社ニコン 観察装置及び観察方法
JP5601098B2 (ja) * 2010-09-03 2014-10-08 ソニー株式会社 蛍光強度補正方法及び蛍光強度算出装置
ES2593464T3 (es) 2010-12-30 2016-12-09 Ventana Medical Systems, Inc. Deposición potenciada de cromógenos utilizando análogos de pirimidina
ES2542106T3 (es) * 2011-04-12 2015-07-30 Tripath Imaging, Inc. Método para preparar videomicroscopía cuantitativa y sistema asociado
JP5725294B2 (ja) * 2011-07-15 2015-05-27 横河電機株式会社 レーザ顕微鏡
SG2014011084A (en) 2011-09-09 2014-04-28 Ventana Med Syst Inc Imaging systems, cassettes, and methods of using the same
EP3764084A1 (en) * 2013-03-12 2021-01-13 Ventana Medical Systems, Inc. Digitally enhanced microscopy for multiplexed histology
CA2899158C (en) * 2013-03-15 2021-05-04 Ventana Medical Systems, Inc. Spectral unmixing
AU2014277034A1 (en) 2013-06-03 2015-10-29 Ventana Medical Systems, Inc. Image adaptive physiologically plausible color separation
JP6304953B2 (ja) * 2013-06-27 2018-04-04 オリンパス株式会社 観察装置
EP3550514B1 (en) 2014-02-21 2020-11-25 Ventana Medical Systems, Inc. Group sparsity model for image unmixing
CA2940439C (en) 2014-02-24 2024-03-19 Ventana Medical Systems, Inc. Quinone methide analog signal amplification
US9797767B2 (en) * 2014-08-26 2017-10-24 General Electric Company Calibration of microscopy systems
CN107209934B (zh) * 2014-12-03 2021-03-30 文塔纳医疗系统公司 用于定量分析异质生物标志物分布的方法、系统和装置
US10078778B2 (en) * 2015-01-15 2018-09-18 Massachusetts Institute Of Technology Systems, methods, and apparatus for in vitro single-cell identification and recovery
WO2017223206A1 (en) * 2016-06-21 2017-12-28 Sri International Hyperspectral imaging methods and apparatuses
JP7021126B2 (ja) 2016-06-28 2022-02-16 ヴェンタナ メディカル システムズ， インク． マルチ色素キノンメチド及びチラミドコンジュゲートによる発色性ｉｈｃ及びｉｓｈ染色のための新規の色
WO2019002281A1 (en) * 2017-06-28 2019-01-03 Ventana Medical Systems, Inc. CALIBRATION OF SYSTEM LEVEL
US10890838B2 (en) * 2018-12-04 2021-01-12 University Of Massachusetts System and methods of fluorescence microscope calibration

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101368908A (zh) * 2007-08-17 2009-02-18 泰肯贸易股份公司 激光扫描装置
CN102165489A (zh) * 2008-09-16 2011-08-24 赫斯托克斯公司 生物标志物表达的可再现量化
WO2014118326A2 (en) * 2013-01-31 2014-08-07 Ventana Medical Systems, Inc. Systems and methods for calibrating, configuring and validating an imaging device or system for multiplex tissue assays

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119151830A (zh) * 2024-08-15 2024-12-17 华南师范大学 一种用于定量荧光共振能量转移显微成像的照明光场校正方法及系统

Also Published As

Publication number Publication date
EP3646006A1 (en) 2020-05-06
JP2020525772A (ja) 2020-08-27
JP2022119771A (ja) 2022-08-17
US11747280B2 (en) 2023-09-05
US20230375477A1 (en) 2023-11-23
JP7368539B2 (ja) 2023-10-24
WO2019002281A1 (en) 2019-01-03
US12320752B2 (en) 2025-06-03
JP7068354B2 (ja) 2022-05-16
US20200080940A1 (en) 2020-03-12
EP3646006B1 (en) 2024-10-23
JP2024024626A (ja) 2024-02-22
EP4488657A1 (en) 2025-01-08

Similar Documents

Publication Publication Date Title
JP7368539B2 (ja) システムレベル較正
JP6995095B2 (ja) 組織試料中で遺伝子を検出するための系
US12126880B2 (en) Digitally enhanced microscopy for multiplexed histology
US10614284B2 (en) Descriptive measurements and quantification of staining artifacts for in situ hybridization
CN107209934B (zh) 用于定量分析异质生物标志物分布的方法、系统和装置
CN112534439A (zh) 用于自动化原位杂交分析的系统
CN107111874A (zh) 用于免疫分数计算中的共表达分析的系统和方法
CN106796145A (zh) 从生物学样本的纯光谱提取
JP2023022233A (ja) 散乱補正を用いた色分離
CN113424050B (zh) 用于计算多通道图像中的自体荧光贡献的系统和方法
CN103123321A (zh) 图像获取装置、图像获取方法和图像获取程序
WO2016189065A1 (en) Method and system for assessing stain quality for in-situ hybridization and immunohistochemistry
Himmel et al. Beyond the H&E: Advanced technologies for in situ tissue biomarker imaging
CN103105381A (zh) 图像获取装置、图像获取方法以及图像获取程序
WO2025188900A1 (en) Systems and methods for biological sample imaging and analysis

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination