CN110776359B - Cassava starch organic annular biocontrol bacterial fertilizer and preparation method thereof - Google Patents

Cassava starch organic annular biocontrol bacterial fertilizer and preparation method thereof Download PDF

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CN110776359B
CN110776359B CN201911037617.XA CN201911037617A CN110776359B CN 110776359 B CN110776359 B CN 110776359B CN 201911037617 A CN201911037617 A CN 201911037617A CN 110776359 B CN110776359 B CN 110776359B
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parts
mass
cassava starch
preparation
bacillus subtilis
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CN110776359A (en
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王杰
雷霆
王凤龙
王静
张成省
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Qiannan Branch Of Guizhou Tobacco Co
Tobacco Research Institute of CAAS
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Qiannan Branch Of Guizhou Tobacco Co
Tobacco Research Institute of CAAS
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Inorganic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a cassava starch organic annular biocontrol bacterial fertilizer, which comprises a part A and a part B, wherein the part A is rod-shaped, and the part B is strip-shaped; wherein the part A comprises 300-500 parts by mass of cassava starch, 100-150 parts by mass of biomass carbon, 2-5 parts by mass of bacillus subtilis and 2-5 parts by mass of bacillus pumilus; the part B comprises cellulose; and a plurality of parts A are arranged along the length direction of the part B and are connected with the part B. The cassava starch is used as a fermentation substrate, the raw material is single, no heavy metal is contained, the stability is good, the safety is high, the physical and chemical properties of the soil can be improved, the proportion of beneficial microorganisms in rhizosphere soil can be adjusted, chemical pesticides and chemical fertilizers can be completely replaced, and the cassava starch is the first choice for import and export bases and pollution-free vegetables.

Description

Cassava starch organic annular biocontrol bacterial fertilizer and preparation method thereof
Technical Field
The invention relates to the field of agricultural fertilizers, in particular to a cassava starch organic annular biocontrol bacterial fertilizer and a preparation method thereof.
Background
The biological control bacterial fertilizer is a fertilizer which organically combines biological control bacteria with biological control activity and nutrient elements by utilizing a biotechnology means, and can synchronously realize soil treatment, biological control of plant diseases and promotion of healthy growth of crops.
The cassava starch contains a large amount of organic matters, and tryptophan in the cassava starch can be decomposed by bacillus subtilis to generate indole so as to promote root growth.
Active substances such as subtilin, polymyxin, nystatin, gramicidin and the like are generated in the growth process of the bacillus subtilis, and the active substances have obvious inhibiting effect on various pathogenic bacteria or conditional pathogenic bacteria of endogenous infection.
The biochar is a substance generated by the thermal conversion of organic biomass in an energy process (such as pyrolysis) of biomass, contains a large amount of carbon and plant nutrients, has a rich pore structure, a large specific surface area and a large number of oxygen-containing active groups on the surface, and can be applied as a soil conditioner to improve the pH value and the content of available nutrients of soil, increase the number and the activity of beneficial microorganisms in the soil and induce plant disease resistance.
At present, the research of cassava starch as a biocontrol bacteria fermentation material and a soil-borne disease inhibitor is not reported, and the conventional fertilizer is mostly granular, needs to be turned over and thrown, is complicated to use and has no pertinence to the drug action. Therefore, it is necessary to provide a further solution to the above problems.
Disclosure of Invention
The invention aims to provide a cassava starch organic annular biocontrol bacterial fertilizer to overcome the defects in the prior art.
In order to solve the technical problems, the technical scheme of the invention is as follows:
an organic annular biological control bacterial fertilizer made of cassava starch comprises a part A and a part B, wherein the part A is rod-shaped, and the part B is strip-shaped; wherein,
the part A comprises 300-500 parts by mass of cassava starch, 100-150 parts by mass of biomass carbon, 2-5 parts by mass of bacillus subtilis and 2-5 parts by mass of bacillus pumilus;
the part B comprises cellulose;
and a plurality of parts A are arranged along the length direction of the part B and are connected with the part B.
Preferably, the part A also comprises 10-20 parts by mass of ammonium humate.
Preferably, the part A comprises 350-400 parts by mass of cassava starch, 120-130 parts by mass of biomass charcoal, 2.5 parts by mass of bacillus subtilis and 2.5 parts by mass of bacillus pumilus.
Preferably, one end of the part A is bent to form an arc.
Preferably, the bending directions of a plurality of the portions a are the same.
Preferably, the part a has a hollow rod shape.
Preferably, the portion a is an arch.
Preferably, one end of the part B is provided with a lug, and the other end of the part B is provided with a through hole matched with the lug.
The invention also provides a preparation method of the cassava starch organic annular biocontrol bacterial fertilizer, which comprises the following steps:
(1) preparation of part a:
a. mixing cassava starch with bacillus subtilis and bacillus pumilus in proportion, adding water, and stirring, wherein the weight ratio of cassava starch to bacillus subtilis to bacillus pumilus is as follows: n is controlled to be 26: 1;
b. putting the mixture into a fermentation tank for fermentation, stirring and cooling at a fixed time, controlling the temperature at 60-80 ℃ for 4-8 days, and measuring the pH value to be 7.2-8.3 to obtain a fermented material;
c. uniformly mixing the fermentation material and the biomass charcoal to obtain part A of raw materials;
d. putting the part A raw materials into a forming machine to be pressed into a rod shape;
(2) preparation of part B: pressing cellulose into a strip shape by a forming machine;
(3) assembling: and a plurality of parts A are arranged along the length direction of the part B and are connected with the part B.
Preferably, the fermentation material is uniformly mixed with the biomass charcoal and 10-20 parts by mass of ammonium humate.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention discloses an organic annular biological control bacterial fertilizer made of cassava starch, which takes the cassava starch as a fermentation substrate, has single raw material, no heavy metal, good stability and high safety, can improve the physical and chemical properties of soil, adjust the proportion of beneficial microorganisms in rhizosphere soil, can completely replace chemical pesticides and chemical fertilizers, and is the first choice for import and export bases and pollution-free vegetables.
(2) The pH value of the invention is between 7.2 and 8.3, which is beneficial to adjusting the soil acidification problem; and the organic matter content of the fertilizer is about 50%, so that the fertilizer is beneficial to conditioning the soil hardening problem, improving the soil aggregate structure and improving the root system environment.
(3) The main fertilizer component A is made into a rod shape, a plurality of the A parts are connected into a whole through the B part and inserted into soil around a root system to directly act on the root system, so that the prevention and control effect is improved; and the stability above the part A is improved through the part B, and the part A is prevented from falling over to cause overall displacement and influence the pesticide and fertilizer application effects.
(4) According to the invention, the tryptophan in the cassava starch is decomposed by using the bacillus subtilis to generate indole, so that the growth of root systems is promoted; meanwhile, the cassava starch provides conditions required by growth for the bacillus subtilis thallus and promotes the bacillus subtilis thallus to generate various active substances so as to inhibit various pathogenic bacteria.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a perspective view of the present invention in use;
FIG. 3 is a perspective view of portion A of an embodiment of the present invention;
fig. 4 is a perspective view of a portion a in another embodiment of the present invention.
Wherein, part 1-A, part 2-B, 11-hollow channel, 12-end face, 21-lug and 22-through hole.
Detailed Description
The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control. The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.
Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.
In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.
Example 1:
the preparation method of the cassava starch organic annular biocontrol bacterial fertilizer comprises the following steps:
(1) preparation of part a:
a. mixing 310 parts by mass of cassava starch, 2 parts by mass of bacillus subtilis and 2 parts by mass of bacillus pumilus in proportion, adding water, and stirring, wherein: n is controlled to be 26: 1.
b. and putting the mixture into a fermentation tank for fermentation, stirring and cooling at a fixed time, controlling the temperature at 60-80 ℃ for 4-8 days, and measuring the pH value to be 7.2-8.3 to obtain the fermented material.
c. And uniformly mixing the fermentation material with 100 parts by mass of biomass charcoal to obtain part A of raw materials.
Preferably, 10-20 parts by mass of ammonium humate is also mixed to promote adhesion of the fermentation material and the biomass charcoal.
d. Putting the part A raw material into a forming machine to be pressed into a rod shape.
As shown in fig. 3, one end of the part a 1 is preferably bent to form a radian, so that the part a can be conveniently inserted into the bottom of the root system and between the root systems; as shown in fig. 4, the part a 1 may also be in an arch structure, that is, the end surface 12 of the part a 1 is in an arc shape, which not only facilitates the part a 1 to be inserted into rhizosphere soil, but also increases the contact area with the rhizosphere soil, and slowly and uniformly releases the active ingredients. The part A1 can also be arranged into a hollow rod shape, namely, the center of the part A is provided with a hollow channel 11, so that the moisture is concentrated from the inside to the outside, and the effective components are slowly brought into rhizosphere soil.
(2) Preparation of part B: pressing cellulose into strip shape by a forming machine, wherein the cellulose can be wood fiber or bamboo fiber, and is slowly decomposed by Bacillus pumilus.
Wherein, for convenient connection and fixation after encircling, part B2 can be pressed into one end through a mould and is provided with a lug 21, the other end is provided with a through hole 22 which is matched with the lug, and the two ends of part B1 are connected and fixed through the matching of the lug 21 and the through hole 22.
(3) Assembling: as shown in figure 1, a plurality of A parts 1 are arranged along the length direction of the B part 2 and connected with the B part 2, the connection can adopt a mode of bonding agent or winding the B part 2, and the like, when in use, as shown in figure 2, the B part is wound into a ring shape, and the A part is inserted into soil around a root system.
The bending directions of the A parts 1 are the same, and after the B part 2 is wound into a ring, the A parts 1 are bent towards the center of the ring so as to wrap the root system among the A parts 1.
Example 2:
a. mixing 350 parts by mass of cassava starch, 2.5 parts by mass of bacillus subtilis and 2.5 parts by mass of bacillus pumilus according to a ratio, adding water, and stirring, wherein: n is controlled to be 26: 1.
c. and uniformly mixing the fermentation material with 120 parts by mass of biomass charcoal to obtain part A of raw materials.
The other steps are the same.
Example 3:
a. mixing 390 parts by mass of cassava starch, 2.6 parts by mass of bacillus subtilis and 2.4 parts by mass of bacillus pumilus according to a proportion, adding water and stirring, and C: n is controlled to be 26: 1.
c. and uniformly mixing the fermentation material with 128 parts by mass of biomass charcoal to obtain part A of raw material.
The other steps are the same.
Example 4:
a. mixing 480 parts by mass of cassava starch, 4 parts by mass of bacillus subtilis and 4.2 parts by mass of bacillus pumilus in proportion, adding water, and stirring, wherein the weight ratio of (A): n is controlled to be 26: 1.
c. and uniformly mixing the fermentation material with 148 parts by mass of biomass charcoal to obtain part A of raw material.
The other steps are the same.
Through determination, the pH values of the fertilizer in the embodiments 1-4 are all between 7.2 and 8.3, and the fertilizer is an alkaline fertilizer, and is beneficial to conditioning the soil acidification problem.
The root system and the stem leaf growth have obvious mutual promotion and mutual inhibition phenomena, and the carbon-nitrogen ratio is an important factor for influence. C: n is 25: 1 is a balance point, which is beneficial to the growth of root systems and stem leaves and promotes the propagation of microorganisms; the carbon-nitrogen ratio is high, which is beneficial to the growth of root systems and inhibits the growth of stems and leaves; the carbon-nitrogen ratio is low, which is beneficial to the growth of stems and leaves and inhibits the growth of root systems. Through determination, the ratio of C to N is 26:1, the carbon-nitrogen ratio is high, and the growth of root systems is facilitated.
In conclusion, the invention discloses an organic annular biological control bacterial fertilizer made of cassava starch, which takes the cassava starch as a fermentation substrate, has the advantages of single raw material, no heavy metal, good stability and high safety, can improve the physical and chemical properties of soil, adjust the proportion of beneficial microorganisms in rhizosphere soil, can completely replace chemical pesticides and chemical fertilizers, is a first choice for manufacturing import and export bases and pollution-free vegetables, and is particularly suitable for tomatoes, garlic and the like.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims (3)

1. The cassava starch organic annular biocontrol bacterial fertilizer is characterized by comprising a part A and a part B, wherein the part B is in a strip shape; wherein,
the part B comprises cellulose;
a plurality of the parts A are arranged along the length direction of the part B and connected with the part B;
the part A is hollow rod-shaped;
one end of the part A is bent to form a radian;
the bending directions of a plurality of the parts A are the same;
one end of the part B is provided with a lug, and the other end of the part B is provided with a through hole matched with the lug;
the part A also comprises 10-20 parts by mass of ammonium humate;
the part A comprises 350-400 parts by mass of cassava starch, 120-130 parts by mass of biomass charcoal, 2.5 parts by mass of bacillus subtilis and 2.5 parts by mass of bacillus pumilus.
2. The cassava starch organic annular biocontrol bacterial fertilizer is characterized by comprising a part A and a part B, wherein the part A is of an arch structure, the part B is of a strip shape, one end of the part B is provided with a bump, and the other end of the part B is provided with a through hole matched with the bump;
the part B comprises cellulose;
a plurality of the parts A are arranged along the length direction of the part B and connected with the part B;
the part A also comprises 10-20 parts by mass of ammonium humate;
the part A comprises 350-400 parts by mass of cassava starch, 120-130 parts by mass of biomass charcoal, 2.5 parts by mass of bacillus subtilis and 2.5 parts by mass of bacillus pumilus.
3. The preparation method of the cassava starch organic ring-shaped biocontrol bacterial fertilizer as claimed in any one of claims 1 or 2, which is characterized by comprising the following steps:
(1) preparation of part a:
a. mixing cassava starch with bacillus subtilis and bacillus pumilus in proportion, adding water, and stirring, wherein the weight ratio of cassava starch to bacillus subtilis to bacillus pumilus is as follows: n is controlled to be 26: 1;
b. putting the mixture into a fermentation tank for fermentation, stirring and cooling at a fixed time, controlling the temperature at 60-80 ℃ for 4-8 days, and measuring the pH value to be 7.2-8.3 to obtain a fermented material;
c. uniformly mixing the fermentation material, biomass charcoal and 10-20 parts by mass of ammonium humate to obtain part A of raw materials;
d. putting the part A raw materials into a forming machine to be pressed into a hollow rod-shaped or arch-shaped structure;
(2) preparation of part B: pressing cellulose into a strip shape by a forming machine;
(3) assembling: and a plurality of parts A are arranged along the length direction of the part B and are connected with the part B.
CN201911037617.XA 2019-10-29 2019-10-29 Cassava starch organic annular biocontrol bacterial fertilizer and preparation method thereof Active CN110776359B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104186078A (en) * 2014-07-30 2014-12-10 青岛嘉禾丰肥业有限公司 Using method of blocky fertilizers capable of saving labor intensity
CN106831273A (en) * 2017-01-24 2017-06-13 北京金都园林绿化有限责任公司 Novel garden trees fertilizer special for organic rod and application method
CN107018737A (en) * 2016-11-07 2017-08-08 王素敏 A kind of application method of drought-resistant fertilizer

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104186078A (en) * 2014-07-30 2014-12-10 青岛嘉禾丰肥业有限公司 Using method of blocky fertilizers capable of saving labor intensity
CN107018737A (en) * 2016-11-07 2017-08-08 王素敏 A kind of application method of drought-resistant fertilizer
CN106831273A (en) * 2017-01-24 2017-06-13 北京金都园林绿化有限责任公司 Novel garden trees fertilizer special for organic rod and application method

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