CN110772544A

CN110772544A - Propolis product and its application in apparatus and wound treatment

Info

Publication number: CN110772544A
Application number: CN201911111502.0A
Authority: CN
Inventors: 杨杨
Original assignee: Jiashan Hongyu Technology Consulting Co Ltd
Current assignee: ZHEJIANG TANZHEN BIOTECHNOLOGY Co.,Ltd.
Priority date: 2019-11-13
Filing date: 2019-11-13
Publication date: 2020-02-11

Abstract

A propolis product contains water less than 10% by moisture measurement. The propolis product is directly or made into medical equipment (such as dressing) to be coated on the wound surface to facilitate the healing of the wound surface, the area of the wound surface healing in unit time is obviously increased, the healing of the wound surface is obviously promoted, and the time for healing the wound surface is shortened.

Description

Propolis product and its application in apparatus and wound treatment

Technical Field

The invention relates to a medical appliance, in particular to a dressing prepared by taking a propolis product as an active ingredient and application in wound treatment.

Background

Trauma is the destruction and damage of human tissues or organs by mechanical factors such as: trauma, burn, bedsore, diabetic foot, and postoperative incision. Wounds not only occur at high rates but also vary widely in degree, and the condition of injury can be severe and complex, even endangering the lives of the injured people.

Not only can the dead or devitalized tissue impede the wound healing process, but the dead tissue or foreign body provides an environment for bacterial growth, increasing the risk of infection and sepsis. On the one hand, the wound should be cleaned in order for the medical staff to correctly assess the wound formed on the tissue and also to be able to see clearly the tissue structure of the wound bed. Debridement, on the other hand, allows the wound to "heal" to an acute state, thereby restarting and accelerating the normal healing process of the wound.

The curette is a common tissue debridement tool in clinic, can thoroughly debride a tissue injury part, and can reduce the degree of secondary injury of the tissue. As a device, the wound cleaning device can only clean dead or inactivated tissues (cell masses) on a wound surface, does not have the effect of promoting tissue healing, and needs to be applied with medicines to promote the healing of the wound surface.

The Zhou Gua disclose a Chinese herbal medicine moistening burn cream (CN1095612A), which can treat various burns and consists of honey, earthworm, and Chinese herbal medicines including pseudo-ginseng, minium, giant knotweed rhizome, bletilla striata, erythrina indica leaves, lysimachia foenum-graecum, musk and camphor, and the proportion of the components is as follows: 78.32-60% of honey, 0.8-1.2% of earthworm, 0.7-1.1% of panax notoginseng, 9-11% of minium, 2-3% of polygonum cuspidatum, 0.9-1.5% of bletilla striata, 8-10% of erythrina indica leaves, 8-9.2% of herba clematidis, 0.08-0.2% of musk, 2.2-2.8% of camphor and the like. The medicine can completely relieve pain within 30 minutes, prevent shock, diminish inflammation, sterilize, resist infection, promote tissue regeneration and hide skin, has no scar after healing I-II degree burn, has no need of skin grafting for III degree burn, and has quick healing, less scar after healing and no various sequelae.

The Chinese patent ZL200810050487.9 discloses an ointment for treating trauma, which can form a layer of protective film on the body surface to isolate and prevent bacteria and viruses from entering from wounds, thereby effectively preventing and controlling infection and achieving the purpose of quick healing. The ointment is prepared from 5 parts of borneol, 150 parts of semen momordicae, 10 parts of calomel, 500 parts of liquid paraffin, 70 parts of white wax, 5 parts of red lead and 200 parts of honey according to the weight ratio. After the ointment is used, the ointment has the characteristics of short treatment course, quick response, obvious treatment effect, complete treatment, no relapse, no toxic or side effect and suitability for clinical popularization. The total effective rate and the cure rate for treating trauma, trauma infection, high fever, edema, local tissue necrosis and complications are 100 percent.

Chinese utility model patent ZL201320706487.6 discloses a honey subsides, still has better viscidity after using 24 hours, and fluid honey can be fine moreover see through isolation rete and wound complex, has promoted the healing of wound. The skin-care wound dressing comprises an elastic PU (polyurethane) base material, a hydrogel matrix layer coated on the base material, a wound-care layer compounded in the center of the hydrogel matrix layer and a lining film, wherein the wound-care layer comprises a honey dry powder layer and an isolation film layer.

Disclosure of Invention

The invention aims to provide a propolis product which is used as an active ingredient and applied to organisms to promote the healing of wound surfaces and shorten the period of wound surface healing.

The invention also aims to provide application of the propolis product in preparing medical equipment for healing wound surfaces.

It is a further object of the present invention to provide a medical device comprising a propolis preparation for application to a wound for promoting healing of the wound.

The invention also aims to provide a dressing containing the propolis product, which is applied to a wound surface and is beneficial to healing of the wound surface.

The term "organism", "animal" or "patient" as used herein refers to humans, wild animals and Livestock (Livestock). The wild animal is an animal which is not artificially domesticated in a natural state. Livestock are animals that are artificially raised to provide a food source, such as: but are not limited to, dogs, cats, mice, rats, hamsters, pigs, rabbits, cows, buffalos, bulls, sheep, goats, geese, chickens, and the like. The "patient" or "organism" to which treatment is administered is preferably a mammal, particularly a human.

As used herein, "treating" or "treatment" refers to inhibiting, suppressing, reducing, ameliorating, slowing, stopping, delaying or reversing the progression or exacerbation of a disease or condition in order to arrest or reduce the occurrence or progression of the disease, and the various indications of the disease, disorder or pathological condition described as maintaining and/or administering include alleviating or reducing symptoms or complications, or curing or eliminating the disease, disorder or condition.

The composition of the invention also comprises various pharmaceutical excipients which are suitable for the contained compound or the composition and are prepared into dosage forms which are beneficial to drug delivery (such as: but are not limited to, patches, suppositories, emulsions, granules, capsules, powders, and the like. When the adjuvant is directly mixed with propolis, it is in dry powder state (liquid content is less than 10 w/w%, especially less than 5 w/w%, preferably 2 w/w%), or coated, pre-coated with propolis (to prevent propolis from imbibing), and mixed with the adjuvants, and made into the following dosage forms: but are not limited to, patches, suppositories, emulsions, creams, gels, granules, capsules, aerosols, sprays, powders, and the like. These pharmaceutical excipients may be those conventionally used in various formulations, such as: but are not limited to, isotonic agents, buffers, excipients, fillers, binders, disintegrants, lubricants, and the like; it may also be selected for use in accordance with the substance, such as: emulsifier, solubilizer, bacteriostatic agent, analgesic and antioxidant. These pharmaceutical excipients may be those conventionally used in various formulations, such as: but are not limited to, isotonic agents, buffers, excipients, fillers, binders, disintegrants, lubricants, and the like; it may also be selected for use in accordance with the substance, such as: emulsifier, solubilizer, bacteriostatic agent, analgesic, antioxidant, etc. These

In liquid formulations, the adjuvants typically include isotonic and buffering agents, as well as the necessary emulsifying agents (e.g., Tween-80, Pluronic and Poloxamer, etc.), solubilizing and bacteriostatic agents, and the like. In addition, the pharmaceutical composition also comprises other pharmaceutically acceptable pharmaceutical excipients, such as: antioxidants, pH modifiers, analgesics, and the like.

The adjuvants used for preparing solid preparations generally include fillers (e.g., starch, sugar powder, dextrin, lactose, compressible starch, microcrystalline cellulose, calcium sulfate, calcium hydrogen phosphate, mannitol, etc.), binders (e.g., ethanol, starch slurry, sodium carboxymethylcellulose, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, hydroxypropyl methyl cellulose, gelatin solution, sucrose solution, and aqueous or alcoholic solution of polyvinylpyrrolidone, etc.), disintegrators (e.g., dry starch, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, crosslinked polyvinylpyrrolidone, and crosslinked sodium carboxymethylcellulose), and lubricants (e.g., magnesium stearate, colloidal silica, talc, hydrogenated vegetable oil, polyethylene glycol 4,000, polyethylene glycol 6,000, magnesium lauryl sulfate, etc.), etc.

The specific methods for various solid formulations vary, such as: the excipients used and the method of manufacture also need to be adjusted, taking the comparison between granules and tablets as an example, granules have similar excipients to tablets but differ in the granulation process. In addition, the granule and capsule are also related, and the prepared granule is mixed with glidant and then encapsulated to obtain the capsule.

The adjuvants used to prepare the emulsion are typically water, oil (such as, but not limited to, lanolin, vegetable oil, sesame oil, peanut oil, cottonseed oil, dimethicone, glycerin, stearic acid, polyoxyethylene stearyl ether, stearyl alcohol, cetearyl alcohol, polyoxyethylene stearic acid, and glyceryl monostearate), emulsifier, penetration enhancer, and necessary preservative.

The water content of the propolis product provided by the invention is less than 10% by water content measurement.

According to the other propolis product provided by the invention, the water content is less than 8% through water content measurement.

According to the other propolis product provided by the invention, the water content is less than 5% through water content measurement.

According to the other propolis product provided by the invention, the water content is less than 2% through water content measurement.

The propolis products provided by the invention are freeze-dried products of propolis, and after being placed for 12 months, the water content is less than 10 percent through water content measurement.

The other propolis product provided by the invention is a freeze-dried propolis product, and the water content of the freeze-dried propolis product is less than 2% through water content measurement, and the water content of the freeze-dried propolis product is less than 10% after the freeze-dried propolis product is placed for 12 months.

The propolis product provided by the invention is prepared by freeze-drying propolis.

The propolis product provided by the invention is prepared by crushing propolis, prefabricating the crushed propolis into a block or sheet shape through a die, and freeze-drying the block or sheet shape.

The other propolis product provided by the invention is freeze-dried powder, the water content of the freeze-dried powder is less than 2% through water content measurement, and the water content of the freeze-dried powder is less than 10% after the freeze-dried powder is placed for 12 months.

The propolis product of the invention can effectively promote blood supply of capillary vessels of wound surfaces and accelerate the repair of the capillary vessels.

The propolis product of the invention is directly coated on the wound surface, which can obviously improve the speed of wound surface healing and shorten the period of wound surface healing.

The propolis product of the invention is used as an active component to prepare medical equipment (such as dressing) for healing wound surfaces of organisms.

The propolis product of the invention is loaded on a substrate (such as cotton, sand line, polyester material and the like) to prepare the dressing, which is then suitable for the wound surface, can obviously improve the speed of wound surface healing and shorten the period of wound surface healing. Such as: coating propolis lyophilized powder in paste form, and coating on gauze to obtain dressing; in addition, the following steps: preparing the propolis composition into liquid, soaking the substrate in the liquid containing the propolis composition, and preparing the substrate into a dressing; the following steps are repeated: preparing propolis composition into solid sheet, applying it on wound surface, and covering with base cloth, such as: gauze, non-woven fabrics, bandages and the like; the method also comprises the following steps: the propolis composition is prepared into liquid, granules, powder, capsules and the like, is directly coated or sprayed on the wound surface, and is coated with base cloth and the like, so that the healing of the wound surface is facilitated.

In order to facilitate application, the propolis composition is prepared into a dressing, the thickness of the propolis composition on a base material is 0.2-2 mm, and the dressing is applied to a wound surface, so that bacteria contamination can be avoided in the process of wound surface treatment, and the propolis composition can be more accurately coated on the wound surface under the action of the base material, and the wound surface can be healed.

The technical scheme of the invention has the following beneficial effects:

the propolis product provided by the invention is prepared in a freeze-drying mode, so that the water content of the propolis product is obviously reduced, and the performance of imbibing and imbibing liquid is improved. The verification proves that the wound healing powder is coated on the wound surface to be beneficial to the healing of the wound surface, the area of the wound surface healing in unit time is obviously increased, the healing of the wound surface is obviously promoted, and the time for the wound surface healing is shortened.

The propolis product provided by the invention is prepared in a freeze-drying mode, the adverse effect of temperature on honey is reduced, the activity retention time is obviously prolonged, the use and the storage of propolis are more convenient, and a wider use method and use are provided.

Drawings

FIG. 1 is a diagram of the healing result of the propolis product on the wound surface of a tested organism.

Detailed Description

The technical scheme of the invention is described in detail in the following with reference to the accompanying drawings. Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.

EXAMPLE 1 preparation of propolis lyophilized product

Grinding and crushing the propolis raw material, placing the crushed propolis raw material in a container, and then placing the container in a freeze-drying device to support a freeze-dried product, such as: a lyophilized product in the form of a tablet or powder.

The water content is less than 2 percent according to the moisture determination method recorded in the 'Chinese pharmacopoeia' 2015 edition. The package was left to stand for 12 months and the moisture content was again determined to be less than 10%.

EXAMPLE 2 preparation of dressing

Loading the prepared propolis freeze-dried product on gauze (or non-woven fabric) to prepare a dressing, and specifically fixing the freeze-dried flaky propolis product on the gauze (or non-woven fabric) to form a propolis layer with the thickness of 0.2-2 mm on the surface of the gauze (or non-woven fabric).

Example 3

The prepared propolis composition was applied to the wound surface of a test animal as follows.

1. Preparing experimental equipment and reagents. Tweezers, scissors, a ruler, an alcohol cotton ball, depilatory cream, chloral hydrate, an injector, a hair shaver, an animal scale and a camera.

2. Mice were divided into 4 groups, respectively: negative control group, commercial propolis, 50% commercial propolis group and propolis product group of the present invention, each group containing 6 propolis.

3. Mice were injected with an anesthetic. The body weight of the mice was weighed, and chloral hydrate was injected in a dose of 1.0ml/20 g.

4. The mice were dehaired. The hair growing on the back of the mouse is removed by a depilator, and then the depilatory cream is uniformly coated on the back of the mouse for depilation.

5. And (5) cutting the wound. A circular wound of 6mm in diameter was cut at the middle of the mouse's back where hair had been removed.

6. And observing and counting the wound healing area.

Commercially available propolis, 50% commercially available propolis cream and the propolis product prepared in this example were applied directly to the wound surface of a living body, and then fixed with gauze (or nonwoven fabric), and the wound surface area of the living body was observed on the 3 rd, 7 th, 9 th and 11 th days after application, as detailed in table 1 below.

TABLE 1

The propolis product of the embodiment is verified to remarkably improve the healing speed of the organism wound, and the dispersion of the healing of the wound of each tested animal is small, so that the propolis product has more remarkable healing effect on the organism wound.

Claims

1. A propolis product is characterized in that the water content is less than 10% by water content measurement.

2. Propolis preparation according to claim 1, characterized in that said water content is less than 8%.

3. Propolis preparation according to claim 1, characterized in that said water content is less than 5%.

4. Propolis preparation according to claim 1, characterized in that said water content is less than 2%.

5. The propolis product according to any one of claims 1 to 4, which is a freeze-dried product of propolis.

6. The propolis product according to any one of claims 1 to 4, wherein the propolis product is a freeze-dried product of propolis, and the water content is less than 10% as measured by water content after standing for 12 months.

7. Use of a propolis product according to any one of claims 1 to 4 in the preparation of a medical device for promoting wound healing.

8. A dressing characterized by comprising the propolis preparation according to any one of claims 1 to 6 as an active ingredient.