CN110812527A

CN110812527A - Propolis composition, apparatus thereof and application thereof in wound treatment

Info

Publication number: CN110812527A
Application number: CN201911111504.XA
Authority: CN
Inventors: 张帅; 杨杨
Original assignee: Zhejiang Shengjian Medical And Health Industry Development Co Ltd
Current assignee: Zhejiang Shengjian Medical And Health Industry Development Co Ltd
Priority date: 2019-11-13
Filing date: 2019-11-13
Publication date: 2020-02-21

Abstract

A propolis composition comprises propolis 50% +/-5%, sodium alginate 15% +/-5% and carboxymethyl cellulose 25% +/-5%. The propolis composition is coated on the wound surface to be beneficial to healing of the wound surface, the area of the wound surface healed in unit time is obviously increased, the healing of the wound surface is obviously promoted, and the time for healing the wound surface is shortened.

Description

Propolis composition, apparatus thereof and application thereof in wound treatment

Technical Field

The invention relates to a medical appliance, in particular to a dressing prepared by taking a propolis composition as an active ingredient and application in wound treatment.

Background

Trauma is the destruction and damage of human tissues or organs by mechanical factors such as: trauma, burn, bedsore, diabetic foot, and postoperative incision. Wounds not only occur at high rates but also vary widely in degree, and the condition of injury can be severe and complex, even endangering the lives of the injured people.

Not only can the dead or devitalized tissue impede the wound healing process, but the dead tissue or foreign body provides an environment for bacterial growth, increasing the risk of infection and sepsis. On the one hand, the wound should be cleaned in order for the medical staff to correctly assess the wound formed on the tissue and also to be able to see clearly the tissue structure of the wound bed. Debridement, on the other hand, allows the wound to "heal" to an acute state, thereby restarting and accelerating the normal healing process of the wound.

The curette is a common tissue debridement tool in clinic, can thoroughly debride a tissue injury part, and can reduce the degree of secondary injury of the tissue. As a device, the wound cleaning device can only clean dead or inactivated tissues (cell masses) on a wound surface, does not have the effect of promoting tissue healing, and needs to be applied with medicines to promote the healing of the wound surface.

The Zhou Gua disclose a Chinese herbal medicine moistening burn cream (CN1095612A), which can treat various burns and consists of honey, earthworm, and Chinese herbal medicines including pseudo-ginseng, minium, giant knotweed rhizome, bletilla striata, erythrina indica leaves, lysimachia foenum-graecum, musk and camphor, and the proportion of the components is as follows: 78.32-60% of honey, 0.8-1.2% of earthworm, 0.7-1.1% of panax notoginseng, 9-11% of minium, 2-3% of polygonum cuspidatum, 0.9-1.5% of bletilla striata, 8-10% of erythrina indica leaves, 8-9.2% of herba clematidis, 0.08-0.2% of musk, 2.2-2.8% of camphor and the like. The medicine can completely relieve pain within 30 minutes, prevent shock, diminish inflammation, sterilize, resist infection, promote tissue regeneration and hide skin, has no scar after healing I-II degree burn, has no need of skin grafting for III degree burn, and has quick healing, less scar after healing and no various sequelae.

The Chinese patent ZL200810050487.9 discloses an ointment for treating trauma, which can form a layer of protective film on the body surface to isolate and prevent bacteria and viruses from entering from wounds, thereby effectively preventing and controlling infection and achieving the purpose of quick healing. The ointment is prepared from 5 parts of borneol, 150 parts of semen momordicae, 10 parts of calomel, 500 parts of liquid paraffin, 70 parts of white wax, 5 parts of red lead and 200 parts of honey according to the weight ratio. After the ointment is used, the ointment has the characteristics of short treatment course, quick response, obvious treatment effect, complete treatment, no relapse, no toxic or side effect and suitability for clinical popularization. The total effective rate and the cure rate for treating trauma, trauma infection, high fever, edema, local tissue necrosis and complications are 100 percent.

Chinese utility model patent ZL201320706487.6 discloses a honey subsides, still has better viscidity after using 24 hours, and fluid honey can be fine moreover see through isolation rete and wound complex, has promoted the healing of wound. The skin-care wound dressing comprises an elastic PU (polyurethane) base material, a hydrogel matrix layer coated on the base material, a wound-care layer compounded in the center of the hydrogel matrix layer and a lining film, wherein the wound-care layer comprises a honey dry powder layer and an isolation film layer.

Disclosure of Invention

The invention aims to provide a propolis composition, and the application of the propolis composition to organisms can promote the healing of wound surfaces and shorten the period of wound surface healing.

The invention also aims to provide application of the propolis composition in preparing a medical device for healing wound surfaces.

It is still another object of the present invention to provide a medical device comprising a propolis composition for application to a wound for promoting healing of the wound.

The invention also aims to provide a dressing containing propolis, which is applied to a wound surface and is beneficial to healing of the wound surface.

The term "organism", "animal" or "patient" as used herein refers to humans, wild animals and Livestock (Livestock). The wild animal is an animal which is not artificially domesticated in a natural state. Livestock are animals that are artificially raised to provide a food source, such as: but are not limited to, dogs, cats, mice, rats, hamsters, pigs, rabbits, cows, buffalos, bulls, sheep, goats, geese, chickens, and the like. The "patient" or "organism" to which treatment is administered is preferably a mammal, particularly a human.

As used herein, "treating" or "treatment" refers to inhibiting, suppressing, reducing, ameliorating, slowing, stopping, delaying or reversing the progression or exacerbation of a disease or condition in order to arrest or reduce the occurrence or progression of the disease, and the various indications of the disease, disorder or pathological condition described as maintaining and/or administering include alleviating or reducing symptoms or complications, or curing or eliminating the disease, disorder or condition.

The composition of the invention also comprises various pharmaceutical excipients which are suitable for the contained compound or the composition and are prepared into dosage forms which are beneficial to drug delivery (such as: but are not limited to, patches, suppositories, emulsions, creams, gels, granules, capsules, aerosols, sprays, powders, and the like. These pharmaceutical excipients may be those conventionally used in various formulations, such as: but are not limited to, isotonic agents, buffers, excipients, fillers, binders, disintegrants, lubricants, and the like; it may also be selected for use in accordance with the substance, such as: emulsifier, solubilizer, bacteriostatic agent, analgesic, antioxidant, etc.

In liquid formulations, the adjuvants typically include isotonic and buffering agents, as well as the necessary emulsifying agents (e.g., Tween-80, Pluronic and Poloxamer, etc.), solubilizing and bacteriostatic agents, and the like. In addition, the pharmaceutical composition also comprises other pharmaceutically acceptable pharmaceutical excipients, such as: antioxidants, pH modifiers, analgesics, and the like.

The adjuvants used for preparing solid preparations generally include fillers (e.g., starch, sugar powder, dextrin, lactose, compressible starch, microcrystalline cellulose, calcium sulfate, calcium hydrogen phosphate, mannitol, etc.), binders (e.g., ethanol, starch slurry, sodium carboxymethylcellulose, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, hydroxypropyl methyl cellulose, gelatin solution, sucrose solution, and aqueous or alcoholic solution of polyvinylpyrrolidone, etc.), disintegrators (e.g., dry starch, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, crosslinked polyvinylpyrrolidone, and crosslinked sodium carboxymethylcellulose), and lubricants (e.g., magnesium stearate, colloidal silica, talc, hydrogenated vegetable oil, polyethylene glycol 4,000, polyethylene glycol 6,000, magnesium lauryl sulfate, etc.), etc.

The specific methods for various solid formulations vary, such as: the excipients used and the method of manufacture also need to be adjusted, taking the comparison between granules and tablets as an example, granules have similar excipients to tablets but differ in the granulation process. In addition, the granule and capsule are also related, and the prepared granule is mixed with glidant and then encapsulated to obtain the capsule.

The adjuvants used to prepare the emulsion are typically water, oil (such as, but not limited to, lanolin, vegetable oil, sesame oil, peanut oil, cottonseed oil, dimethicone, glycerin, stearic acid, polyoxyethylene stearyl ether, stearyl alcohol, cetearyl alcohol, polyoxyethylene stearic acid, and glyceryl monostearate), emulsifier, penetration enhancer, and necessary preservative.

The invention provides a propolis composition, which comprises 50 w/w% +/-5 w/w% of propolis, 15 w/w% +/-5 w/w% of sodium alginate and 25 w/w% +/-5 w/w% of carboxymethyl cellulose.

The other propolis composition provided by the invention consists of 50 w/w% +/-5 w/w% of propolis, 15 w/w% +/-5 w/w% of sodium alginate, 25 w/w% +/-5 w/w% of carboxymethyl cellulose and the balance of solvent.

The propolis composition provided by the invention takes water as a solvent preferentially.

The composition containing the propolis is loaded on a base material (such as cotton, sand lines, polyester materials and the like) to prepare the dressing, is suitable for the wound, can obviously improve the speed of wound healing and shorten the period of wound healing. Such as: preparing propolis composition into paste, and coating on gauze to obtain dressing; in addition, the following steps: preparing the propolis composition into liquid, soaking the substrate in the liquid containing the propolis composition, and preparing the substrate into a dressing; the following steps are repeated: preparing propolis composition into solid sheet, applying it on wound surface, and covering with base cloth, such as: gauze, non-woven fabrics, bandages and the like; the method also comprises the following steps: the propolis composition is prepared into liquid, granules, powder, capsules and the like, is directly coated or sprayed on the wound surface, and is coated with base cloth and the like, so that the healing of the wound surface is facilitated.

In order to facilitate application, the propolis composition is prepared into a dressing, the thickness of the propolis composition on a base material is 0.2-2 mm, and the dressing is applied to a wound surface, so that bacteria contamination can be avoided in the process of wound surface treatment, and the propolis composition can be more accurately coated on the wound surface under the action of the base material, and the wound surface can be healed.

The technical scheme of the invention has the following beneficial effects:

according to the propolis composition provided by the invention, sodium alginate and CMC are used, and after verification, the propolis composition is coated on a wound surface to facilitate the healing of the wound surface, the area of the wound surface healing in unit time is remarkably increased, the healing of the wound surface is remarkably promoted, and the time for healing the wound surface is shortened.

The propolis composition provided by the invention has the advantages that the wound surface is isolated, the barrier effect is achieved, and meanwhile, good air permeability and moisture permeability are provided for the wound surface.

Drawings

Fig. 1 is a diagram of the healing result of the propolis composition on the wound surface of a tested organism.

Detailed Description

The technical scheme of the invention is described in detail in the following with reference to the accompanying drawings. Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.

EXAMPLE 1 preparation of propolis composition

Mixing 50 w/w% of propolis, 15 w/w% of sodium alginate and 25 w/w% of carboxymethyl cellulose into paste according to the parts by weight to obtain the propolis composition of the embodiment.

EXAMPLE 2 preparation of dressing

Loading the prepared propolis composition on gauze (or non-woven fabric) to prepare a dressing, and specifically coating the pasty propolis composition on the gauze (or non-woven fabric) to form a propolis composition layer with the thickness of 0.2-2 mm on the surface of the gauze (or non-woven fabric).

Example 3

The prepared propolis composition was applied to the wound surface of a test animal as follows.

1. Preparing experimental equipment and reagents. Tweezers, scissors, a ruler, an alcohol cotton ball, depilatory cream, chloral hydrate, an injector, a hair shaver, an animal scale and a camera.

2. Mice were divided into 3 groups: negative control group, group containing 50% propolis composition and group containing 100% propolis composition, each group containing 6.

3. Mice were injected with an anesthetic. The body weight of the mice was weighed, and chloral hydrate was injected in a dose of 1.0ml/20 g.

4. The mice were dehaired. The hair growing on the back of the mouse is removed by a depilator, and then the depilatory cream is uniformly coated on the back of the mouse for depilation.

5. And (5) cutting the wound. A circular wound of 6mm in diameter was cut at the middle of the mouse's back where hair had been removed.

6. And observing and counting the wound healing area.

The wound surface of the tested animal is coated with 50% propolis composition and 100% propolis composition, and then fixed with gauze (or non-woven fabric), and the wound surface area of the organism is observed on the 1 st, 5 th and 11 th days after application, which is detailed in the following table 1.

TABLE 1

The propolis product of the embodiment is verified to remarkably improve the healing speed of the organism wound, and the dispersion of the healing of the wound of each tested animal is small, so that the propolis product has more remarkable healing effect on the organism wound.

Claims

1. A propolis composition is characterized by comprising 50 w/w% +/-5 w/w% of propolis, 15 w/w% +/-5 w/w% of sodium alginate and 25 w/w% +/-5 w/w% of carboxymethyl cellulose.

2. A propolis composition is characterized by comprising 50 w/w% +/-5 w/w% of propolis, 15 w/w% +/-5 w/w% of sodium alginate, 25 w/w% +/-5 w/w% of carboxymethyl cellulose and the balance of solvent.

3. The propolis composition according to claim 2, wherein the solvent is water.

4. Use of a propolis composition according to any one of claims 1 to 3 in the preparation of a medical device for promoting wound healing.

5. A dressing characterized by comprising the propolis composition according to any one of claims 1 to 3 as an active ingredient.