CN102524623A - Bee composition and preparation method thereof - Google Patents
Bee composition and preparation method thereof Download PDFInfo
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- CN102524623A CN102524623A CN2012100027912A CN201210002791A CN102524623A CN 102524623 A CN102524623 A CN 102524623A CN 2012100027912 A CN2012100027912 A CN 2012100027912A CN 201210002791 A CN201210002791 A CN 201210002791A CN 102524623 A CN102524623 A CN 102524623A
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Abstract
The invention relates to a bee composition and a preparation method thereof. The bee composition mainly comprises the following components of lyophilized royal jelly powder, lyophilized queen bee larva powder, propolis powder and pearl powder in weight ratio of (20-60):(20-60):(5-15):(5-15). The preparation method of the bee composition comprises the following steps of: firstly, preparing the lyophilized royal jelly powder; secondly, preparing the lyophilized queen bee larva powder; thirdly, preparing the propolis powder; fourthly, preparing the pearl powder and fifthly, respectively sterilizing the treated lyophilized royal jelly powder, lyophilized queen bee larva powder, propolis powder and pearl powder; and then mixing according to the proportion. According to the bee composition disclosed by the invention, the immunologic function of a human body can be enhanced; various components can better exert the efficiencies of each substance under the synergistic effect; and the bee composition can be taken for a long term, is convenient to take and eat, is convenient to carry, is simple in preparation method and is suitable for industrial production.
Description
Technical field
The present invention relates to health product technology field, especially relate to a kind of honeybee composition and method of making the same.
Background technology
Royal jelly has very strong biologically active, have increase immunologic function, delay senility, functions such as cancer-resisting, hypoglycemic, hypotensive, antifatigue, it is remarkable particularly to regulate the immunology effect again.The content of vitamin A and D is abundant in the queen bee nit, and the content of vitamin D is the 6130-7430 international unit in every gram queen bee nit, surpasses the content of cod-liver oil, yolk.The chemical composition of propolis is very complicated, also contains trace elements such as multivitamin, amino acid, cadmium, iron, zinc.Active component in the pearl such as taurine have anti-inflammatory, hemostasis, analgesic pharmacological action.But royal jelly is not only distinguished the flavor of sour, puckery, hot, bad adaptability, and will freeze and deposit, to open ice when edible, food is got inconvenience.
Summary of the invention
The present invention is for providing a kind of honeybee composition and method of making the same, and this honeybee composition can improve the immunologic function of human body, can take for a long time, and process acts synergistically between the various component, can better bring into play the effect of every kind of material; And it is convenient that this product is got food, is easy to carry; Its preparation method is simple, is suitable for suitability for industrialized production.
The technical problem that the present invention solved adopts following technical scheme to realize:
A kind of honeybee composition, mainly be made up of following component: royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, its weight ratio are 20-60: 20-60: 5-15: 5-15.
Above-mentioned royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, its preferred weight ratio is 30-50: 30-50: 8-12: 8-12.
Above-mentioned royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, its preferred weight ratio is 20: 60: 15: 5.
Above-mentioned royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, its preferred weight ratio is 4: 4: 1: 1.
A kind of honeybee preparation of compositions method comprises:
(1) preparation of royal jelly freeze-dried powder
The fresh royal jelly of treating freeze-drying is mixed with sterile distilled water, filter; With the filter residue rinsing after filtering, isolate the crystallization of 10-hydroxyl caproleic acid, grind, add in the said filtrate filtered; To filtrate afterwards moves in the container, and the slurry layer thickness is controlled at 8mm-10mm, freezes at subzero 40 ℃-subzero 80 ℃; Product after will freezing is put into freeze drying box, and vacuum pressure is controlled to be the 1.33-2.0 handkerchief, starts heating system again, makes the product temperature after freezing rise to subzero 25 ℃-subzero 30 ℃, and makes steam pressure be controlled at the 13-15 handkerchief, time remaining 10-15h; Adjusting temperature afterwards is 30 ℃-40 ℃, and steam pressure is the 13-15 handkerchief, and the time is 4-6 hour; Product after will handling is then pulverized, and sieves, and obtains royal jelly freeze-dried powder;
(2) preparation of queen bee nit freeze-dried powder
The bright queen bee nit of treating freeze-drying is mixed with sterile distilled water, grind, sieve; Product after will sieving afterwards moves in the container, and the slurry layer thickness is controlled at 8mm-10mm, freezes at subzero 40 ℃-subzero 80 ℃; Product after will freezing is put into freeze drying box, and vacuum pressure is controlled to be the 1.33-2.0 handkerchief, starts heating system again, makes the product temperature after freezing rise to subzero 25 ℃-subzero 30 ℃, and makes steam pressure be controlled at the 13-15 handkerchief, time remaining 10-15h; Adjusting temperature afterwards is 30 ℃-40 ℃, and steam pressure is the 13-15 handkerchief, and the time is 4-6 hour; Product after will handling is then pulverized, and sieves, and obtains the queen bee nit freeze-dried powder;
(3) preparation of propolis powder
Propolis at subzero 18 ℃-subzero 25 ℃ of freezing 2-5 hour, is pulverized afterwards and obtained former propolis powder, and the temperature of working environment is lower than 15 ℃; With former propolis powder and ethanol according to volume ratio 1: 2-4 mixes; Left standstill at room temperature stirring and leaching 2-5 hour, and got supernatant and filter, make the temperature of filtrating reduce to 2-5 ℃ afterwards, get supernatant again, after the beautiful yarn of 4-6 layer filters, get the propolis alcohol extract through beautiful yarn; The propolis alcohol extract is added in the container, connect the cooling water of condenser, connect hot water heating distiller, the boiling of propolis alcohol extract, alcohol vapour gets into condenser by conduit, stops to concentrate, and promptly gets propolis cream, and cooling obtains pure propolis; At subzero 18 ℃-subzero 25 ℃ of freezing 2-5 hour, crushing screening obtains pure propolis powder afterwards with pure propolis, and the temperature of working environment is lower than 15 ℃;
(4) preparation of pearl powder
With crushing pearl, sieve, obtain pearl powder;
(5) royal jelly freeze-dried powder after the above-mentioned processing, queen bee nit freeze-dried powder, propolis powder and pearl powder are sterilized respectively; Sterilization is that 20-60: 20-60: 5-15: 5-15 mixes according to weight ratio afterwards, promptly gets the honeybee composition.
Fresh royal jelly in the said step (1) and sterile distilled water are preferably according to volume ratio 1-2: 1-2 mixes.
Bright queen bee nit in the said step (2) and sterile distilled water are preferably according to volume ratio 1-2: 1-2 mixes.
Ethanol in the said step (3) is that mass percent is 95% ethanol.
Filter residue in the said step (3) and sediment merge, and add and preceding ethanol with amount again, stir, and leave standstill, and isolate supernatant, are ready to use in the lixiviate of next group propolis powder; These sediments add again and preceding ethanol with amount, stir quiet amount; Isolate supernatant, be ready to use in the lixiviate that filter residue and sediment after the lixiviate of next group propolis powder merge thing, again the about sediment of remainder is added again and preceding with the ethanol of measuring; Stir, leave standstill, isolate supernatant; Be ready to use in the lixiviate of the preceding sediment of next group, so proceed, until separated liquid supernatant almost be colourless till; Each separated liquid supernatant all is used for the lixiviate of the preceding sediment of next group, adopts the multistage lixiviate operation of this adverse current, can isolate the active ingredient in the propolis to greatest extent, also can reduce the energy consumption that the propolis alcohol extract concentrates.
The steam pressure of said step (1) or (2) is preferably 13.33 handkerchiefs.
Make the temperature of filtrating reduce to 2-5 ℃ of method in the said step (3) in the coil pipe immersion filtrating of adopting logical chilled brine.
30W ultra violet lamp 30-60min is adopted in sterilization in the said step (5), and the thickness of tiling is preferably below the 0.5cm.
To pass through the honeybee composition that above-mentioned preparation method makes and be made into capsule.
Sieve in the said step to be preferably and cross 100 mesh sieves respectively.
Above-mentioned beautiful yarn is the beautiful yarn of 100 orders.
Honeybee composition of the present invention is carried out the function assessment test; The result shows: the honeybee composition can significantly improve mice serum hemolytic antibody product level, NK cells in mice activity and Turnover of Mouse Peritoneal Macrophages and engulf the phagocytic activity of chicken red blood cell and prove, honeybee composition of the present invention has the effect that improves immunologic function.
Honeybee composition of the present invention carries out the toxicology test result and shows, the acute poison of attacking: phase I large and small mouse acute oral toxicity median lethal dose, bisexuality are pressed acute toxicity grading criteria and are judged that these article belong to non-toxic type all greater than the 10.0g/kg body weight; Second stage Ames experimental result is negative; Mouse Bone marrow cells micronucleus experiment and mouse sperm deformity experiment are all negative.The no mutagenicity of these article of prompting.30 days feeding trials of rat, each item index are not all seen the overt toxicity reaction, and its maximum no-effect dose is the 3.00g/Kg body weight.Above result shows that it is safe that peak of the present invention composition eats for a long time.
Contain protein more than 12 kinds, 20 several amino acids, 10 multivitamins in the royal jelly, especially the B family vitamin is abundant especially, tonic food still not, and have significant beauty functions.Contained a large amount of active matter mass-energy kinase system excretes lipofuscin, reduces its content.Therefore royal jelly have the immunologic function of increasing, delay senility, functions such as cancer-resisting, hypoglycemic, hypotensive, antifatigue, it is remarkable particularly to regulate the immunology effect again.
The water content 78-82% of queen bee nit, the total nitrogen of freeze-drying larva powder reaches more than 8%, and protein content accounts for 48% of dry, and fat content accounts for about 15%.The content of vitamin A and D is abundant in the queen bee nit, and vitamin D content is the 6130-7430 international unit in every gram larva, surpasses the content of cod-liver oil, yolk.Experiment shows.Queen bee nit to mouse cell immunity, humoral immunity and non-specific immune function by humidification.
The chemical composition of propolis and complicacy thereof, the effect of pharmacology aspect also are many-sided, as antibiotic, pain relieving, anesthesia, promotion regeneration, antitumor, anti-oxidant, improve cardiovascular function etc., but mainly be influence to the human immune system.
The main component of pearl is CaCO
3, also contain amino acid in 22, contain element among Cu, Zn, Mn, Sr, Ba, the Al etc. 20 in addition, have that tranquilizing mind, yin-nourishing relieve dizziness, high fever, infantile convulsions, epilepsy, etc., effects such as phlegm is fallen in heat-clearing, detoxify and promote granulation; Modern medicine shows, main inorganic constituents CaCO in the pearl
3, get into the Ca of human body behind the mild hydrolysis
+In the body harmless; And can promote the vigor of the triphosphoric acid glycosides biocatalyst Adenosine triphosphate thuja acid of the human body energy; Active skull cap components in the pearl such as taurine have anti-inflammatory, hemostasis, analgesic pharmacological action, and pearl also can be used as the health products raw material of immunoregulation effect.
The beneficial effect that the present invention has is:
(1) honeybee composition of the present invention can improve the immunologic function of human body, can take for a long time, and process acts synergistically between the various component, can better bring into play the effect of every kind of material; And it is convenient that this product is got food, is easy to carry;
(2) preparation method of the present invention is simple, is suitable for suitability for industrialized production.
The specific embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect and be easy to understand and understand, below in conjunction with concrete diagram, further set forth the present invention.
Embodiment 1
A kind of honeybee composition mainly is made up of following component: royal jelly freeze-dried powder 20g, queen bee nit freeze-dried powder 60g, propolis powder 15g and pearl powder 5g.
A kind of honeybee preparation of compositions method comprises:
(1) preparation of royal jelly freeze-dried powder
(a) preliminary treatment of royal jelly will be treated 1: 1 by volume the adding sterile distilled water of fresh royal jelly of freeze-drying earlier, through 100
Mesh sieve filters to remove impurity; Again filter residue is used the distilled water rinsing, isolate 10 monohydroxy decylenic acid crystallizations, and levigate by filtering desired order number, return mixing in the royal jelly filtrating;
(b) royal jelly that the pre-freeze of royal jelly is good with preliminary treatment moves in the square plate, is controlled at 8mm～10mm to the slurry layer thickness, sends into freezer then, and quick freezing becomes solid with subsequent use under-40 ℃ cryogenic conditions;
(c) lyophilization of royal jelly will quick freezing become the royal jelly of solid to move in the freeze drying box under-40 ℃ of cryogenic conditions; Start vavuum pump; Its vacuum pressure is maintained about 1.33 handkerchiefs; Start heating system again, make the temperature of freezing royal jelly rise to-25 ℃, and vapour pressure in the freeze drying box is maintained about 13.33 handkerchiefs by-40 ℃; When queen bee slurry temperature was-25 ℃ in the freeze drying box, the temperature of condenser should be-50 ℃, congealed into ice rapidly to guarantee the steam of discharging in the freeze drying box, reduced the load of oil-sealed rotary pump, and the lyophilization of royal jelly is carried out smoothly; The lyophilization process approximately needs 12 hours; The moisture of royal jelly can only be reduced to about 10% after this process, make it to drop to about 2%, also need carry out parsing-desiccation;
(d) parsing-desiccation of royal jelly still contains 10% moisture in the royal jelly after lyophilization, and the royal jelly of water content can't be by permanent preservation like this; Must be through improving heating-up temperature and keeping higher vacuum; The hydrone that is adsorbed is parsed from loose royal jelly under bigger parsing motive force, is about 2% to continue being dried to water content, and the temperature of parsing-desiccation is advisable with 30 ℃; The highest can not be above 40 ℃; Vapour pressure in the drying box should maintain about 13.33 handkerchiefs, and to keep bigger parsing motive force, this dry run needs 4-6 hour;
(e) royal jelly after the pulverizing freeze drying of royal jelly freeze-dried powder is pulverized through ball mill for loose block, obtains royal jelly freeze-dried powder after 100 mesh sieves sieve; The hygroscopicity of freeze-dried powder is very strong, for preventing to pollute and the steam intrusion, must pass through purified treatment to the air that gets into operating room, and its relative humidity should be lower than 20%; (2) queen bee nit freeze-dried powder preparation:
(a) preliminary treatment of queen bee nit is frozen-18 ℃ of refrigerator and cooled by the queen bee nit taking-up back of bee farm production at once, and the back is transported in the freezer with refrigerator car and preserved; With the ratio adding sterile distilled water of the bright queen bee nit of treating freeze-drying in 1: 1, colloid mill grinds, and the strainer filtering of warp 100 mesh sieves obtains the queen bee nit slurry to remove impurity;
(b) pre-freeze of queen bee nit slurry, lyophilization, parsing-desiccation, disintegrating process are produced identical with royal jelly freeze-dried powder; (3) preparation of propolis powder
(a) former propolis freezing, pulverize in refrigerator propolis is placed on-18 ℃, freezing more than 2 hours, adopt ball mill to pulverize, the temperature of working environment keeps below 15 ℃;
(b) the ethanol lixiviate of former propolis is 95% ethanol with former propolis powder and mass percent by 1: 4 proportioning, places in the stainless steel agitator, leaves standstill after 2 hours in stirring and leaching under the room temperature; Get supernatant and filter through 100 order thin,tough silk yarns, filtrating is put basin and is waited to concentrate, and filter residue and sediment merge; Add again and preceding 95% ethanol, stir, leave standstill with amount; Isolate supernatant, be ready to use in the lixiviate of next group propolis powder; These sediments add again and preceding 95% ethanol with amount, stir quiet amount; Isolate supernatant, be ready to use in the lixiviate that filter residue and sediment after the lixiviate of next group propolis powder merge thing, again the about sediment of remainder is added again and preceding with 95% ethanol of measuring; Stir; Leave standstill, isolate supernatant, be ready to use in the lixiviate of the preceding sediment of next group; So proceed, until separated liquid supernatant almost be colourless till; Each separated liquid supernatant all is used for the lixiviate of the preceding sediment of next group, adopts the multistage lixiviate operation of this adverse current, can isolate the active ingredient in the propolis to greatest extent, also can reduce the energy consumption that the propolis alcohol extract concentrates;
(c) propolis filtrating to be concentrated in the storage filling is put in the filtration of propolis alcohol extract; Though filter through 100 order thin,tough silk yarns, still have the cold alcohol-insoluble substance of part to exist, also need adopt the coil pipe of logical chilled brine to immerse wherein; Its temperature is reduced to below 5 ℃, to quicken the deposition of this part cold alcohol-insoluble substance; And then get supernatant, after 4 layer of 100 order thin,tough silk yarn filters, pump into concentration tank;
(d) recovery that reaches ethanol that concentrates of propolis alcohol extract adds propolis ethanol liquid in the distiller, connects the cooling water of condenser, connects hot water heating distiller; The boiling of propolis alcohol extract; The ethanol steam gets into condenser by conduit, after condensation, becomes ethanol liquid, flows into receiver; When stopping to concentrate, should close hot water earlier, treat moments later to close cooling water again, emit propolis cream, cooling gets pure propolis;
(e) pulverize that pure propolis is put into-18 ℃ of refrigerators is freezing, pulverize pure propolis piece with ball mill, 100 order mesh screens filter, and obtain pure propolis powder; The temperature of working environment keeps below 15 ℃;
(4) preparation of pearl powder
With crushing pearl, sieve, obtain pearl powder;
(5) royal jelly freeze-dried powder after the above-mentioned processing, queen bee nit freeze-dried powder, propolis powder and pearl powder are tiled in respectively in the drip pan, tiling thickness is less than 0.5mm, and irradiation-sterilize is 30 minutes under the 30W uviol lamp; Dry powder is turned over appearance pave, under uviol lamp, shone 30 minutes again, reach the sterilization purpose 2 times repeatedly;
(6) mixing, can:
Under 100,000 grades of environment, behind royal jelly freeze-dried powder 20g, queen bee nit freeze-dried powder 60g, propolis powder 15g and pearl powder 5g mixing after the sterilization; Can, polishing, packing is finished product after the assay was approved; The whole process of production room temperature is controlled at 18-24 ℃, and relative humidity maintains 45-55%.
Embodiment 2
A kind of honeybee composition mainly is made up of following component: royal jelly freeze-dried powder 60g, queen bee nit freeze-dried powder 30g, propolis powder 5g and pearl powder 5g.
A kind of honeybee preparation of compositions method comprises:
(2) preparation of royal jelly freeze-dried powder
(a) preliminary treatment of royal jelly will be treated 1: 2 by volume the adding sterile distilled water of fresh royal jelly of freeze-drying earlier, filter to remove impurity through 100 mesh sieves; Again filter residue is used the distilled water rinsing, isolate 10 monohydroxy decylenic acid crystallizations, and levigate by filtering desired order number, return mixing in the royal jelly filtrating;
(b) royal jelly that the pre-freeze of royal jelly is good with preliminary treatment moves in the square plate, is controlled at 8mm～10mm to the slurry layer thickness, sends into freezer then, and quick freezing becomes solid with subsequent use under-80 ℃ cryogenic conditions;
(c) lyophilization of royal jelly will quick freezing become the royal jelly of solid to move in the freeze drying box under-80 ℃ of cryogenic conditions; Start vavuum pump; Its vacuum pressure is maintained about 2.0 handkerchiefs; Start heating system again, make the temperature of freezing royal jelly rise to-30 ℃, and vapour pressure in the freeze drying box is maintained about 13.33 handkerchiefs by-80 ℃; When queen bee slurry temperature was-25 ℃ in the freeze drying box, the temperature of condenser should be-50 ℃, congealed into ice rapidly to guarantee the steam of discharging in the freeze drying box, reduced the load of oil-sealed rotary pump, and the lyophilization of royal jelly is carried out smoothly; The lyophilization process approximately needs 12 hours; The moisture of royal jelly can only be reduced to about 10% after this process, make it to drop to about 2%, also need carry out parsing-desiccation;
(d) parsing-desiccation of royal jelly still contains 10% moisture in the royal jelly after lyophilization, and the royal jelly of water content can't be by permanent preservation like this; Must be through improving heating-up temperature and keeping higher vacuum; The hydrone that is adsorbed is parsed from loose royal jelly under bigger parsing motive force, is about 2% to continue being dried to water content, and the temperature of parsing-desiccation is advisable with 30 ℃; The highest can not be above 40 ℃; Vapour pressure in the drying box should maintain about 13.33 handkerchiefs, and to keep bigger parsing motive force, this dry run needs 4-6 hour;
(e) royal jelly after the pulverizing freeze drying of royal jelly freeze-dried powder is pulverized through ball mill for loose block, obtains royal jelly freeze-dried powder after 100 mesh sieves sieve; The hygroscopicity of freeze-dried powder is very strong, for preventing to pollute and the steam intrusion, must pass through purified treatment to the air that gets into operating room, and its relative humidity should be lower than 20%;
(2) queen bee nit freeze-dried powder preparation:
(a) preliminary treatment of queen bee nit is frozen-25 ℃ of refrigerator and cooled by the queen bee nit taking-up back of bee farm production at once, and the back is transported in the freezer with refrigerator car and preserved; With the ratio adding sterile distilled water of the bright queen bee nit of treating freeze-drying in 2: 1, colloid mill grinds, and the strainer filtering of warp 100 mesh sieves obtains the queen bee nit slurry to remove impurity;
(b) pre-freeze of queen bee nit slurry, lyophilization, parsing-desiccation, disintegrating process are produced identical with royal jelly freeze-dried powder;
(3) preparation of propolis powder
(a) former propolis freezing, pulverize in refrigerator propolis is placed on-25 ℃, freezing 5 hours, adopt ball mill to pulverize, the temperature of working environment keeps below 15 ℃;
(b) the ethanol lixiviate of former propolis is 90% ethanol with former propolis powder and mass percent by 1: 4 proportioning, places in the stainless steel agitator, leaves standstill after 2 hours in stirring and leaching under the room temperature; Get supernatant and filter through 100 order thin,tough silk yarns, filtrating is put basin and is waited to concentrate, and filter residue and sediment merge; Add again and preceding 90% ethanol, stir, leave standstill with amount; Isolate supernatant, be ready to use in the lixiviate of next group propolis powder; These sediments add again and preceding 90% ethanol with amount, stir quiet amount; Isolate supernatant, be ready to use in the lixiviate that filter residue and sediment after the lixiviate of next group propolis powder merge thing, again the about sediment of remainder is added again and preceding with 95% ethanol of measuring; Stir; Leave standstill, isolate supernatant, be ready to use in the lixiviate of the preceding sediment of next group; So proceed, until separated liquid supernatant almost be colourless till; Each separated liquid supernatant all is used for the lixiviate of the preceding sediment of next group, adopts the multistage lixiviate operation of this adverse current, can isolate the active ingredient in the propolis to greatest extent, also can reduce the energy consumption that the propolis alcohol extract concentrates;
(c) propolis filtrating to be concentrated in the storage filling is put in the filtration of propolis alcohol extract; Though filter through 100 order thin,tough silk yarns, still have the cold alcohol-insoluble substance of part to exist, also need adopt the coil pipe of logical chilled brine to immerse wherein; Its temperature is reduced to about 2 ℃, to quicken the deposition of this part cold alcohol-insoluble substance; And then get supernatant, after 6 layer of 100 order thin,tough silk yarn filters, pump into concentration tank;
(d) recovery that reaches ethanol that concentrates of propolis alcohol extract adds propolis ethanol liquid in the distiller, connects the cooling water of condenser, connects hot water heating distiller; The boiling of propolis alcohol extract; The ethanol steam gets into condenser by conduit, after condensation, becomes ethanol liquid, flows into receiver; When stopping to concentrate, should close hot water earlier, treat moments later to close cooling water again, emit propolis cream, cooling gets pure propolis;
(e) pulverize that pure propolis is put into-25 ℃ of refrigerators is freezing, pulverize pure propolis piece with ball mill, 100 order mesh screens filter, and obtain pure propolis powder; The temperature of working environment keeps below 15 ℃;
(4) preparation of pearl powder
With crushing pearl, sieve, obtain pearl powder;
(5) royal jelly freeze-dried powder after the above-mentioned processing, queen bee nit freeze-dried powder, propolis powder and pearl powder are tiled in respectively in the drip pan, tiling thickness 0.2mm, irradiation-sterilize is 60 minutes under the 30W uviol lamp; Dry powder is turned over appearance pave, under uviol lamp, shone 30 minutes again, reach the sterilization purpose 2 times repeatedly;
(6) mixing, can:
Under 100,000 grades of environment, behind royal jelly freeze-dried powder 60g, queen bee nit freeze-dried powder 30g, propolis powder 5g and pearl powder 5g mixing after the sterilization; Can, polishing, packing is finished product after the assay was approved; The whole process of production room temperature is controlled at 18-24 ℃, and relative humidity maintains 45-55%.
Embodiment 3
A kind of honeybee composition mainly is made up of following component: royal jelly freeze-dried powder 40g, queen bee nit freeze-dried powder 40g, propolis powder 10g and pearl powder 10g.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; The present invention is not restricted to the described embodiments; That describes in the foregoing description and the specification just explains principle of the present invention; Under the prerequisite that does not break away from spirit and scope of the invention, the present invention also has various changes and modifications, and these variations and improvement all fall in the scope of the invention that requires protection.The present invention requires protection domain to be defined by appending claims and equivalent thereof.
Claims (10)
1. a honeybee composition is characterized in that, mainly is made up of following component: royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, its weight ratio are 20-60: 20-60: 5-15: 5-15.
2. a kind of honeybee composition according to claim 1 is characterized in that, said royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, and its weight ratio is 30-50: 30-50: 8-12: 8-12.
3. a kind of honeybee composition according to claim 1 is characterized in that, said royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, and its weight ratio is 20: 60: 15: 5.
4. a kind of honeybee composition according to claim 1 is characterized in that, said royal jelly freeze-dried powder, queen bee nit freeze-dried powder, propolis powder and pearl powder, and its weight ratio is 4: 4: 1: 1.
5. honeybee preparation of compositions method comprises:
(1) will treat that the fresh royal jelly of freeze-drying mixes with sterile distilled water, filter; With the filter residue rinsing after filtering, isolate the crystallization of 10-hydroxyl caproleic acid, grind, add in the said filtrate filtered; To filtrate afterwards moves in the container, and the slurry layer thickness is controlled at 8mm-10mm, freezes at subzero 40 ℃-subzero 80 ℃; Product after will freezing is put into freeze drying box, and vacuum pressure is controlled to be the 1.33-2.0 handkerchief, starts heating system again, makes the product temperature after freezing rise to subzero 25 ℃-subzero 30 ℃, and makes steam pressure be controlled at the 13-15 handkerchief, time remaining 10-15h; Adjusting temperature afterwards is 30 ℃-40 ℃, and steam pressure is the 13-15 handkerchief, and the time is 4-6 hour; Product after will handling is then pulverized, and sieves, and obtains royal jelly freeze-dried powder;
(2) the bright queen bee nit that will treat freeze-drying mixes with sterile distilled water, grinds, and sieves; Product after will sieving afterwards moves in the container, and the slurry layer thickness is controlled at 8mm-10mm, freezes at subzero 40 ℃-subzero 80 ℃; Product after will freezing is put into freeze drying box, and vacuum pressure is controlled to be the 1.33-2.0 handkerchief, starts heating system again, makes the product temperature after freezing rise to subzero 25 ℃-subzero 30 ℃, and makes steam pressure be controlled at the 13-15 handkerchief, time remaining 10-15h; Adjusting temperature afterwards is 30 ℃-40 ℃, and steam pressure is the 13-15 handkerchief, and the time is 4-6 hour; Product after will handling is then pulverized, and sieves, and obtains the queen bee nit freeze-dried powder;
(3) with propolis at subzero 18 ℃-subzero 25 ℃ of freezing 2-5 hour, pulverize afterwards and obtain former propolis powder, the temperature of working environment is lower than 15 ℃; With former propolis powder and ethanol according to volume ratio 1: 2-4 mixes; Left standstill at room temperature stirring and leaching 2-5 hour, and got supernatant and filter, make the temperature of filtrating reduce to 2-5 ℃ afterwards, get supernatant again, after the beautiful yarn of 4-6 layer filters, get the propolis alcohol extract through beautiful yarn; The propolis alcohol extract is added in the container, connect the cooling water of condenser, connect hot water heating distiller, the boiling of propolis alcohol extract, alcohol vapour gets into condenser by conduit, stops to concentrate, and promptly gets propolis cream, and cooling obtains pure propolis; At subzero 18 ℃-subzero 25 ℃ of freezing 2-5 hour, crushing screening obtains pure propolis powder afterwards with pure propolis, and the temperature of working environment is lower than 15 ℃;
(4) with crushing pearl, sieve, obtain pearl powder;
(5) royal jelly freeze-dried powder after the above-mentioned processing, queen bee nit freeze-dried powder, propolis powder and pearl powder are sterilized respectively; Sterilization is that 20-60: 20-60: 5-15: 5-15 mixes according to weight ratio afterwards, promptly gets the honeybee composition.
6. a kind of honeybee preparation of compositions method according to claim 5 is characterized in that, the fresh royal jelly in the said step (1) and sterile distilled water are according to volume ratio 1-2: 1-2 mixes; Bright queen bee nit in the said step (2) and sterile distilled water are according to volume ratio 1-2: 1-2 mixes.
7. according to claim 5 or 6 described a kind of honeybee preparation of compositions methods, it is characterized in that the ethanol in the said step (3) is that mass percent is 95% ethanol.
8. according to claim 5 or 6 described a kind of honeybee preparation of compositions methods, it is characterized in that the steam pressure of said step (1) or (2) is 13.33 handkerchiefs.
9. according to claim 5 or 6 described a kind of honeybee preparation of compositions methods, it is characterized in that, make the temperature of filtrating reduce to 2-5 ℃ of method in the said step (3) in the coil pipe immersion filtrating of adopting logical chilled brine.
10. according to claim 5 or 6 described a kind of honeybee preparation of compositions methods, it is characterized in that 30W ultra violet lamp 30-60min is adopted in sterilization in the said step (5), the thickness of tiling is below the 0.5cm.
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| CN104799283A (en) * | 2015-04-28 | 2015-07-29 | 贾群影 | Method for preparing queen bee larva into food |
| CN105768004A (en) * | 2016-03-24 | 2016-07-20 | 明光市昊昊蜂业有限公司 | Preparation technology of royal jelly |
| CN108902852A (en) * | 2018-06-07 | 2018-11-30 | 刘运鸿 | A kind of bee product composition and preparation method thereof |
| CN110772544A (en) * | 2019-11-13 | 2020-02-11 | 嘉善鸿域科技咨询有限公司 | Propolis product and its application in apparatus and wound treatment |
| CN113397086A (en) * | 2021-05-11 | 2021-09-17 | 上海老滇凰生物科技有限公司 | Refrigeration, preservation and purification equipment and method for queen bee larva and royal jelly |
| CN114190561A (en) * | 2021-12-09 | 2022-03-18 | 北京中蜜科技发展有限公司 | Microencapsulated royal jelly enzymolysis polypeptide, preparation method thereof and solid powder containing microencapsulated royal jelly enzymolysis polypeptide |
| CN114947090A (en) * | 2022-03-24 | 2022-08-30 | 上海老滇凰生物科技有限公司 | Lyophilized tablet of queen bee larva and preparation and storage method thereof |
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