CN110771746A - Animal feed additive and feed containing same - Google Patents
Animal feed additive and feed containing same Download PDFInfo
- Publication number
- CN110771746A CN110771746A CN201911033641.6A CN201911033641A CN110771746A CN 110771746 A CN110771746 A CN 110771746A CN 201911033641 A CN201911033641 A CN 201911033641A CN 110771746 A CN110771746 A CN 110771746A
- Authority
- CN
- China
- Prior art keywords
- solution
- container
- mass
- feed additive
- component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000019730 animal feed additive Nutrition 0.000 title claims abstract description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 100
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 89
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 74
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims abstract description 66
- 238000001704 evaporation Methods 0.000 claims abstract description 59
- RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical compound NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 claims abstract description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 239000004471 Glycine Substances 0.000 claims abstract description 38
- 230000008020 evaporation Effects 0.000 claims abstract description 36
- 238000003756 stirring Methods 0.000 claims abstract description 35
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims abstract description 33
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 31
- 235000015165 citric acid Nutrition 0.000 claims abstract description 31
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 claims abstract description 31
- 235000010334 sodium propionate Nutrition 0.000 claims abstract description 31
- 239000004324 sodium propionate Substances 0.000 claims abstract description 31
- 229960003212 sodium propionate Drugs 0.000 claims abstract description 31
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 31
- 235000019731 tricalcium phosphate Nutrition 0.000 claims abstract description 31
- 229940078499 tricalcium phosphate Drugs 0.000 claims abstract description 31
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims abstract description 31
- 229960002173 citrulline Drugs 0.000 claims abstract description 25
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims abstract description 24
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims abstract description 24
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000001630 malic acid Substances 0.000 claims abstract description 24
- 235000011090 malic acid Nutrition 0.000 claims abstract description 24
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 claims abstract description 24
- 239000001433 sodium tartrate Substances 0.000 claims abstract description 24
- 229960002167 sodium tartrate Drugs 0.000 claims abstract description 24
- 235000011004 sodium tartrates Nutrition 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000008569 process Effects 0.000 claims abstract description 22
- 230000032683 aging Effects 0.000 claims abstract description 12
- 238000010438 heat treatment Methods 0.000 claims abstract description 12
- 230000010355 oscillation Effects 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 229960004106 citric acid Drugs 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 210
- 235000006545 Ziziphus mauritiana Nutrition 0.000 claims description 44
- 235000008529 Ziziphus vulgaris Nutrition 0.000 claims description 44
- 239000007791 liquid phase Substances 0.000 claims description 33
- 238000002156 mixing Methods 0.000 claims description 29
- 235000007877 Diospyros australis Nutrition 0.000 claims description 27
- 240000002577 Prunus nigra Species 0.000 claims description 27
- 235000010875 Prunus nigra Nutrition 0.000 claims description 27
- 235000018288 Vitex doniana Nutrition 0.000 claims description 27
- 239000003674 animal food additive Substances 0.000 claims description 26
- 239000008367 deionised water Substances 0.000 claims description 22
- 229910021641 deionized water Inorganic materials 0.000 claims description 22
- 235000019441 ethanol Nutrition 0.000 claims description 22
- 239000007864 aqueous solution Substances 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 238000005520 cutting process Methods 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 11
- 239000007789 gas Substances 0.000 claims description 11
- 238000002137 ultrasound extraction Methods 0.000 claims description 11
- 238000001291 vacuum drying Methods 0.000 claims description 11
- 244000126002 Ziziphus vulgaris Species 0.000 claims description 6
- 229910001873 dinitrogen Inorganic materials 0.000 claims description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 4
- RHGKLRLOHDJJDR-UHFFFAOYSA-N Ndelta-carbamoyl-DL-ornithine Natural products OC(=O)C(N)CCCNC(N)=O RHGKLRLOHDJJDR-UHFFFAOYSA-N 0.000 claims description 2
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims description 2
- 206010012735 Diarrhoea Diseases 0.000 abstract description 11
- 239000003242 anti bacterial agent Substances 0.000 abstract description 7
- 229940088710 antibiotic agent Drugs 0.000 abstract description 7
- 241000282887 Suidae Species 0.000 abstract description 6
- 239000000654 additive Substances 0.000 abstract description 3
- 230000000996 additive effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 230000002411 adverse Effects 0.000 abstract description 2
- 240000000038 Ziziphus mauritiana Species 0.000 description 39
- 230000000052 comparative effect Effects 0.000 description 15
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 11
- 235000019733 Fish meal Nutrition 0.000 description 11
- 235000019764 Soybean Meal Nutrition 0.000 description 11
- 240000008042 Zea mays Species 0.000 description 11
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 11
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 11
- 239000011575 calcium Substances 0.000 description 11
- 229910052791 calcium Inorganic materials 0.000 description 11
- 235000005822 corn Nutrition 0.000 description 11
- 239000004467 fishmeal Substances 0.000 description 11
- 150000003839 salts Chemical class 0.000 description 11
- 239000004455 soybean meal Substances 0.000 description 11
- 235000015099 wheat brans Nutrition 0.000 description 11
- 238000011049 filling Methods 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000006806 disease prevention Effects 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000193403 Clostridium Species 0.000 description 1
- 206010012335 Dependence Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002207 thermal evaporation Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/195—Antibiotics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an animal feed additive, which comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the preparation method of the component A comprises the following steps: 1) dissolving glycine and L-citrulline in water to form a solution, dropwise adding ethanolamine into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution during stirring, heating the solution to 80-90 ℃ after the solution is heated, evaporating the solution until the volume of the solution is 1/5-1/4 before evaporation, and performing ultrasonic oscillation treatment during the evaporation process; 2) and after evaporation, adding absolute ethyl alcohol, citric acid, sodium propionate and tricalcium phosphate into the solution, aging at 40-50 ℃ for more than 8 hours, and evaporating water in the solution to dryness to obtain the component A. According to the invention, the self-made additive is added into the feed to replace antibiotics, so that the diarrhea rate of pigs is remarkably reduced, the potential safety hazard of food is avoided, and the adverse effect of the antibiotics is eliminated.
Description
Technical Field
The invention belongs to the technical field of feed processing, and particularly relates to an animal feed additive and a feed containing the same.
Background
The feed additive is a general term of special substances added for meeting certain special needs of animals in the processes of feed processing, storage, blending and use, and is an important component of compound feed. The classification of feed additives is generally divided according to composition and use. According to the use of the feed additive, the feed additive can be divided into five types, such as nutrition (amino acid, vitamin and trace element), growth promotion and disease prevention (antibiotics and various disease prevention and treatment agents), feed degradation prevention (antioxidant and mildew preventive), feed flavor and product quality improvement (flavoring agent, spice agent and coloring agent), physiological function adjustment (hormone, enzyme and sedative), and the like.
The diarrhea of the pigs mainly occurs in the piglet stage, the diarrhea is mainly caused by escherichia coli, salmonella and clostridium welchii, the most common is yellow dysentery and white dysentery caused by the escherichia coli and the salmonella, and the problem is solved by using antibiotics generally for treating the diarrhea at present, but the mode causes food safety addiction.
Disclosure of Invention
The invention provides an animal feed additive, which comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the preparation method of the component A comprises the following steps:
1) dissolving glycine and L-citrulline in water to form a solution, dropwise adding ethanolamine into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution during stirring, heating the solution to 80-90 ℃ after the solution is heated, evaporating the solution until the volume of the solution is 1/5-1/4 before evaporation, and performing ultrasonic oscillation treatment during the evaporation process;
2) and after evaporation, adding absolute ethyl alcohol, citric acid, sodium propionate and tricalcium phosphate into the solution, aging at 40-50 ℃ for more than 8 hours, and evaporating water in the solution to dryness to obtain the component A.
Further, the extraction method of the jujube-black plum extract comprises the following steps:
(1) taking fresh black brin pulp and dry fructus Jujubae, cutting, mixing, placing the mixture in a closed container, charging nitrogen gas into the container until the pressure in the container is not less than 3 standard atmospheric pressures, maintaining the pressure for more than 10min, rapidly extracting the gas in the container, reducing the pressure in the container to below 0.1 standard atmospheric pressure within 10s, maintaining the pressure for more than 5min, and opening the container to balance the internal and external air pressures;
(2) adding deionized water into a container, performing ultrasonic extraction for more than 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, adding an ethanol aqueous solution into the solution, uniformly stirring, standing for more than 12h, centrifuging, and performing vacuum drying to constant weight to obtain the jujube-black plum extract.
Further, in the animal feed additive, the components are as follows by weight: 100 parts of component A, 2-10 parts of malic acid, 1-6 parts of sodium tartrate and 30-40 parts of Chinese date-black plum extract.
Further, in the step 1), the mixing mass ratio of glycine to L-citrulline is glycine: l-citrulline =1: 0.4-0.7, the concentration of glycine in a solution formed by dissolving glycine and L-citrulline in water is 50-60 g/L, and the adding mass of zinc nitrate and copper chloride/mass of the solution are respectively as follows: 20-30 g/500mL of zinc nitrate solution, 5-10 g/500mL of copper chloride solution, and the ultrasonic vibration treatment time is not less than 5 min.
In the step 2), the addition mass and the solution ratio of the absolute ethyl alcohol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 20-30 mL/100mL of the absolute ethyl alcohol, 1-2 g/100mL of the citric acid, 0.3-0.8 g/100mL of the sodium propionate and 0.1-0.2 g/100mL of the tricalcium phosphate.
Further, the mixing mass ratio of the fresh black plum pulp to the dried Chinese dates is as follows: the dry Chinese dates =10: 3-20.
Further, in the step (2), the adding amount of the deionized water is more than 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates; the volume percentage content of the ethanol in the ethanol water solution is 60-70 percent, the balance is water, and the added mass of the ethanol water solution is 1/3 of the mass of the liquid phase after the pressure concentration.
The invention also provides a feed which contains the feed additive.
According to the technical scheme, the invention has the beneficial effects that: according to the invention, the self-made additive is added into the feed to replace antibiotics, so that the diarrhea rate of pigs is remarkably reduced, the potential safety hazard of food is avoided, and the adverse effect of the antibiotics is eliminated.
Detailed Description
The following is a detailed description with reference to examples:
example 1
An animal feed additive comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the components are as follows by weight: 100 parts of component A, 2 parts of malic acid, 1 part of sodium tartrate and 30 parts of Chinese date-black plum extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.4 to form a solution, wherein the concentration of glycine in the solution is 50 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: heating the solution to 80-90 ℃ after feeding, evaporating to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and adding anhydrous ethanol, citric acid, sodium propionate and tricalcium phosphate into the solution after evaporation, wherein the adding mass and the solution ratio of the anhydrous ethanol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 20mL/100mL of the anhydrous ethanol solution, 1g/100mL of the citric acid solution, 0.3g/100mL of the sodium propionate solution and 0.1g/100mL of the tricalcium phosphate solution, aging at 40-50 ℃ for 8 hours, and evaporating the water in the solution to dryness to obtain the component A.
The extraction method of the Chinese date-black plum extract comprises the following steps:
(1) taking fresh black plum pulp and dried Chinese dates, cutting up the fresh black plum pulp and the dried Chinese dates, and mixing the fresh black plum pulp and the dried Chinese dates in a mass ratio: mixing the dried Chinese dates according to the ratio of =10:3, placing the mixture in a closed container, filling nitrogen into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, then rapidly extracting the gas in the container, reducing the pressure in the container to be less than 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae-Bluegum extract.
The feed additive prepared in the embodiment is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Example 2
An animal feed additive comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the components are as follows by weight: the composition comprises 100 parts of component A, 5 parts of malic acid, 3 parts of sodium tartrate and 32 parts of Chinese date-black plum extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.5 to form a solution, wherein the concentration of glycine in the solution is 52 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: heating the solution to 80-90 ℃ after feeding of 23g/500mL of zinc nitrate solution and 7g/500mL of copper chloride solution, carrying out thermal evaporation to 1/5 of the volume of the solution before evaporation, and carrying out ultrasonic oscillation treatment in the evaporation process;
2) and after evaporation is finished, adding anhydrous ethanol, citric acid, sodium propionate and tricalcium phosphate into the solution, wherein the adding mass and the solution ratio of the anhydrous ethanol, the citric acid, the sodium propionate and the tricalcium phosphate are 23mL/100mL of anhydrous ethanol solution, 1.2g/100mL of citric acid solution, 0.4g/100mL of sodium propionate solution and 0.13g/100mL of tricalcium phosphate solution respectively, aging at 40-50 ℃ for 8 hours, and evaporating water in the solution to dryness to obtain the component A.
The extraction method of the Chinese date-black plum extract comprises the following steps:
(1) taking fresh black plum pulp and dried Chinese dates, cutting up the fresh black plum pulp and the dried Chinese dates, and mixing the fresh black plum pulp and the dried Chinese dates in a mass ratio: mixing the dried Chinese dates according to the ratio of =10:8, placing the mixture in a closed container, filling nitrogen into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, then rapidly extracting the gas in the container, reducing the pressure in the container to be less than 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae-Bluegum extract.
The feed additive prepared in the embodiment is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Example 3
An animal feed additive comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the components are as follows by weight: 100 parts of component A, 6 parts of malic acid, 4 parts of sodium tartrate and 35 parts of Chinese date-black plum extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.6 to form a solution, wherein the concentration of glycine in the solution is 56 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: 25g/500mL of zinc nitrate solution and 8g/500mL of copper chloride solution, heating the solution to 80-90 ℃ after the addition, thermally evaporating the solution to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and after evaporation is finished, adding absolute ethyl alcohol, citric acid, sodium propionate and tricalcium phosphate into the solution, wherein the adding mass and the solution ratio of the absolute ethyl alcohol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 25mL/100mL of the absolute ethyl alcohol solution, 1.6g/100mL of the citric acid solution, 0.6g/100mL of the sodium propionate solution and 0.16g/100mL of the tricalcium phosphate solution, aging at 40-50 ℃ for 8 hours, and evaporating water in the solution to dryness to obtain the component A.
The extraction method of the Chinese date-black plum extract comprises the following steps:
(1) taking fresh black plum pulp and dried Chinese dates, cutting up the fresh black plum pulp and the dried Chinese dates, and mixing the fresh black plum pulp and the dried Chinese dates in a mass ratio: mixing the dried Chinese dates according to the ratio of =10:13, placing the mixture in a closed container, filling nitrogen into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, then rapidly extracting the gas in the container, reducing the pressure in the container to be less than 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae-Bluegum extract.
The feed additive prepared in the embodiment is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Example 4
An animal feed additive comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the components are as follows by weight: 100 parts of component A, 8 parts of malic acid, 5 parts of sodium tartrate and 38 parts of Chinese date-black plum extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.6 to form a solution, wherein the concentration of glycine in the solution is 59 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: 27g/500mL of zinc nitrate solution and 8g/500mL of copper chloride solution, heating the solution to 80-90 ℃ after the addition, thermally evaporating the solution to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and adding anhydrous ethanol, citric acid, sodium propionate and tricalcium phosphate into the solution after evaporation, wherein the adding mass and the solution ratio of the anhydrous ethanol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 28mL/100mL of anhydrous ethanol solution, 1.7g/100mL of citric acid solution, 0.7g/100mL of sodium propionate solution and 0.18g/100mL of tricalcium phosphate solution, aging at 40-50 ℃ for 8 hours, and evaporating the water in the solution to dryness to obtain the component A.
The extraction method of the Chinese date-black plum extract comprises the following steps:
(1) taking fresh black plum pulp and dried Chinese dates, cutting up the fresh black plum pulp and the dried Chinese dates, and mixing the fresh black plum pulp and the dried Chinese dates in a mass ratio: mixing the dried Chinese dates in a ratio of =10:17, placing the mixture in a closed container, filling nitrogen into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, then rapidly extracting the gas in the container, reducing the pressure in the container to be less than 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae-Bluegum extract.
The feed additive prepared in the embodiment is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Example 5
An animal feed additive comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the components are as follows by weight: 100 parts of component A, 10 parts of malic acid, 6 parts of sodium tartrate and 40 parts of Chinese date-black plum extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water to form a solution with the concentration of glycine of 60g/L, wherein the ratio of L-citrulline =1: 0.7; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: 30g/500mL of zinc nitrate solution and 10g/500mL of copper chloride solution, heating the solution to 80-90 ℃ after the addition, thermally evaporating the solution to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and after evaporation is finished, adding anhydrous ethanol, citric acid, sodium propionate and tricalcium phosphate into the solution, wherein the adding mass and the solution ratio of the anhydrous ethanol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 30mL/100mL of the anhydrous ethanol solution, 2g/100mL of the citric acid solution, 0.8g/100mL of the sodium propionate solution and 0.2g/100mL of the tricalcium phosphate solution, aging at 40-50 ℃ for 8 hours, and evaporating water in the solution to dryness to obtain the component A.
The extraction method of the Chinese date-black plum extract comprises the following steps:
(1) taking fresh black plum pulp and dried Chinese dates, cutting up the fresh black plum pulp and the dried Chinese dates, and mixing the fresh black plum pulp and the dried Chinese dates in a mass ratio: mixing the dried Chinese dates according to the ratio of =10:20, placing the mixture in a closed container, filling nitrogen into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, then rapidly extracting the gas in the container, reducing the pressure in the container to be less than 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae-Bluegum extract.
The feed additive prepared in the embodiment is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Comparative example 1
An animal feed additive comprises 6 parts of malic acid, 4 parts of sodium tartrate and 35 parts of Chinese date-black plum extract by weight.
The extraction method of the Chinese date-black plum extract comprises the following steps:
(1) taking fresh black plum pulp and dried Chinese dates, cutting up the fresh black plum pulp and the dried Chinese dates, and mixing the fresh black plum pulp and the dried Chinese dates in a mass ratio: mixing the dried Chinese dates according to the ratio of =10:13, placing the mixture in a closed container, filling nitrogen into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, then rapidly extracting the gas in the container, reducing the pressure in the container to be less than 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae-Bluegum extract.
The feed additive prepared in the comparative example is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Comparative example 2
An animal feed additive comprises a component A, malic acid and sodium tartrate, wherein the component A comprises the following components in parts by weight: 100 parts of component A, 6 parts of malic acid and 4 parts of sodium tartrate.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.6 to form a solution, wherein the concentration of glycine in the solution is 56 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: 25g/500mL of zinc nitrate solution and 8g/500mL of copper chloride solution, heating the solution to 80-90 ℃ after the addition, thermally evaporating the solution to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and after evaporation is finished, adding absolute ethyl alcohol, citric acid, sodium propionate and tricalcium phosphate into the solution, wherein the adding mass and the solution ratio of the absolute ethyl alcohol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 25mL/100mL of the absolute ethyl alcohol solution, 1.6g/100mL of the citric acid solution, 0.6g/100mL of the sodium propionate solution and 0.16g/100mL of the tricalcium phosphate solution, aging at 40-50 ℃ for 8 hours, and evaporating water in the solution to dryness to obtain the component A.
The feed additive prepared in the comparative example is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Comparative example 3
An animal feed additive comprises a component A, malic acid, sodium tartrate and a Chinese date extract, wherein the components are as follows by weight: 100 parts of component A, 6 parts of malic acid, 4 parts of sodium tartrate and 35 parts of Chinese date extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.6 to form a solution, wherein the concentration of glycine in the solution is 56 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: 25g/500mL of zinc nitrate solution and 8g/500mL of copper chloride solution, heating the solution to 80-90 ℃ after the addition, thermally evaporating the solution to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and after evaporation is finished, adding absolute ethyl alcohol, citric acid, sodium propionate and tricalcium phosphate into the solution, wherein the adding mass and the solution ratio of the absolute ethyl alcohol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 25mL/100mL of the absolute ethyl alcohol solution, 1.6g/100mL of the citric acid solution, 0.6g/100mL of the sodium propionate solution and 0.16g/100mL of the tricalcium phosphate solution, aging at 40-50 ℃ for 8 hours, and evaporating water in the solution to dryness to obtain the component A.
The extraction method of the Chinese date extract comprises the following steps:
(1) cutting dried fructus Jujubae, placing in a closed container, charging nitrogen gas into the container until the pressure in the container is not less than 3 standard atmospheric pressures, maintaining the pressure for 10min, rapidly extracting the gas from the container, reducing the pressure in the container to below 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into the container, wherein the adding amount of the deionized water is 6 times of the mass of the dried Chinese dates in the container, carrying out ultrasonic extraction for 10min, filtering to obtain a liquid phase, carrying out pressure concentration on the liquid phase to half of the original volume, adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae extract.
The feed additive prepared in the comparative example is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Comparative example 4
An animal feed additive comprises a component A, malic acid, sodium tartrate and a black burlin extract, and the components are as follows by weight: 100 parts of component A, 6 parts of malic acid, 4 parts of sodium tartrate and 35 parts of black plum extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.6 to form a solution, wherein the concentration of glycine in the solution is 56 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: 25g/500mL of zinc nitrate solution and 8g/500mL of copper chloride solution, heating the solution to 80-90 ℃ after the addition, thermally evaporating the solution to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and after evaporation is finished, adding absolute ethyl alcohol, citric acid, sodium propionate and tricalcium phosphate into the solution, wherein the adding mass and the solution ratio of the absolute ethyl alcohol, the citric acid, the sodium propionate and the tricalcium phosphate are respectively 25mL/100mL of the absolute ethyl alcohol solution, 1.6g/100mL of the citric acid solution, 0.6g/100mL of the sodium propionate solution and 0.16g/100mL of the tricalcium phosphate solution, aging at 40-50 ℃ for 8 hours, and evaporating water in the solution to dryness to obtain the component A.
The extraction method of the black brin extract comprises the following steps:
(1) cutting fresh black plum pulp, placing in a closed container, charging nitrogen gas into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, rapidly extracting the gas in the container, reducing the pressure in the container to below 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external air pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the mass of the black plum pulp in the container, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the black brin extract.
The feed additive prepared in the comparative example is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Comparative example 5
An animal feed additive comprises a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the components are as follows by weight: 100 parts of component A, 6 parts of malic acid, 4 parts of sodium tartrate and 35 parts of Chinese date-black plum extract.
The preparation method of the component A comprises the following steps:
1) glycine according to the mixing mass ratio: dissolving glycine and L-citrulline in water at a ratio of L-citrulline =1:0.6 to form a solution, wherein the concentration of glycine in the solution is 56 g/L; adding ethanolamine dropwise into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution in the stirring process, wherein the adding mass of the zinc nitrate and the adding mass of the copper chloride/the mass of the solution are respectively as follows: 25g/500mL of zinc nitrate solution and 8g/500mL of copper chloride solution, heating the solution to 80-90 ℃ after the addition, thermally evaporating the solution to 1/5 of the volume of the solution before evaporation, and performing ultrasonic oscillation treatment in the evaporation process;
2) and after the evaporation is finished, adding absolute ethyl alcohol into the solution, wherein the addition mass of the absolute ethyl alcohol and the solution ratio are 25mL/100mL of the absolute ethyl alcohol, aging for 8 hours at 40-50 ℃, and evaporating the water in the solution to dryness to obtain the component A.
The extraction method of the Chinese date-black plum extract comprises the following steps:
(1) taking fresh black plum pulp and dried Chinese dates, cutting up the fresh black plum pulp and the dried Chinese dates, and mixing the fresh black plum pulp and the dried Chinese dates in a mass ratio: mixing the dried Chinese dates according to the ratio of =10:13, placing the mixture in a closed container, filling nitrogen into the container until the pressure in the container is more than or equal to 3 standard atmospheric pressures, maintaining the pressure for 10min, then rapidly extracting the gas in the container, reducing the pressure in the container to be less than 0.1 standard atmospheric pressure within 10s, maintaining the pressure for 5min, and opening the container to balance the internal and external atmospheric pressures;
(2) adding deionized water into a container, wherein the adding amount of the deionized water is 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates, performing ultrasonic extraction for 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, and adding an ethanol aqueous solution into the solution, wherein the adding mass of the ethanol aqueous solution is 1/3 of the mass of the liquid phase after the pressure concentration. Stirring uniformly, standing for 12h, centrifuging, and vacuum drying to constant weight to obtain the fructus Jujubae-Bluegum extract.
The feed additive prepared in the embodiment is added into a test pig feed to form a pig basic ration, and the mass ratio of each component in the pig basic ration is 65% of corn, 23% of soybean meal, 5% of wheat bran, 5.2% of fish meal, 1% of calcium hydrophosphate, 0.3% of salt and 0.5% of feed additive.
Example 6
The method comprises the following steps of averagely dividing 110 piglets of 20-day weaning day age into 11 groups, feeding 10 piglets in each group, controlling the temperature of a pigsty within the range of 25 +/-2 ℃, and carrying out conventional immunization and parasite expelling. Wherein 10 groups of the feed are respectively fed with the basic ration for the pigs prepared in the examples 1-5 and the comparative examples 1-5, the other group of the feed is used as a control group, and the mass ratio of each group of the feed for the test pigs in the control group is respectively 65.5 percent of corn, 23 percent of soybean meal, 5 percent of wheat bran, 5.2 percent of fish meal, 1 percent of calcium hydrophosphate and 0.3 percent of salt. The feeding period is 40 days.
The diarrhea condition of the piglets is observed in the feeding period, and the diarrhea rate of each group of piglets is calculated, wherein the diarrhea rate = the diarrhea frequency of the piglets/(the feeding period multiplied by 10), multiplied by 100%, and the results are shown in table 1.
TABLE 1
| Test group | Rate of diarrhea/%) |
| Control group | 9.5% |
| Example 1 | 4.25% |
| Example 2 | 4.5% |
| Example 3 | 4.0% |
| Example 4 | 4.5% |
| Example 5 | 4.75% |
| Comparative example 1 | 7.0% |
| Comparative example 2 | 8.5% |
| Comparative example 3 | 6.0% |
| Comparative example 4 | 5.0% |
| Comparative example 5 | 6.25% |
As can be seen from the table 1, the self-made additive is added into the feed to replace the action of antibiotics, so that the diarrhea rate of the pigs is remarkably reduced, and the effect is remarkable.
The technical solutions provided by the present invention are described in detail above, and for those skilled in the art, the ideas according to the embodiments of the present invention may be changed in the specific implementation manners and the application ranges, and in summary, the content of the present description should not be construed as limiting the present invention.
Claims (8)
1. An animal feed additive is characterized by comprising a component A, malic acid, sodium tartrate and a Chinese date-black plum extract, wherein the preparation method of the component A comprises the following steps:
1) dissolving glycine and L-citrulline in water to form a solution, dropwise adding ethanolamine into the solution to adjust the pH of the solution to be within the range of 12-13, continuously stirring the solution, adding zinc nitrate and copper chloride into the solution during stirring, heating the solution to 80-90 ℃ after the solution is heated, evaporating the solution until the volume of the solution is 1/5-1/4 before evaporation, and performing ultrasonic oscillation treatment during the evaporation process;
2) and after evaporation, adding absolute ethyl alcohol, citric acid, sodium propionate and tricalcium phosphate into the solution, aging at 40-50 ℃ for more than 8 hours, and evaporating water in the solution to dryness to obtain the component A.
2. The animal feed additive of claim 1, wherein the extraction method of the jujube-black plum extract comprises:
(1) taking fresh black brin pulp and dry fructus Jujubae, cutting, mixing, placing the mixture in a closed container, charging nitrogen gas into the container until the pressure in the container is not less than 3 standard atmospheric pressures, maintaining the pressure for more than 10min, rapidly extracting the gas in the container, reducing the pressure in the container to below 0.1 standard atmospheric pressure within 10s, maintaining the pressure for more than 5min, and opening the container to balance the internal and external air pressures;
(2) adding deionized water into a container, performing ultrasonic extraction for more than 10min, filtering to obtain a liquid phase, performing pressure concentration on the liquid phase to half of the original volume, adding an ethanol aqueous solution into the solution, uniformly stirring, standing for more than 12h, centrifuging, and performing vacuum drying to constant weight to obtain the jujube-black plum extract.
3. The animal feed additive as claimed in claim 1, wherein the animal feed additive comprises the following components in parts by weight: 100 parts of component A, 2-10 parts of malic acid, 1-6 parts of sodium tartrate and 30-40 parts of Chinese date-black plum extract.
4. The animal feed additive according to claim 1, wherein in the step 1), the mixing mass ratio of glycine to L-citrulline is glycine: l-citrulline =1: 0.4-0.7, the concentration of glycine in a solution formed by dissolving glycine and L-citrulline in water is 50-60 g/L, and the adding mass of zinc nitrate and copper chloride/mass of the solution are respectively as follows: 20-30 g/500mL of zinc nitrate solution, 5-10 g/500mL of copper chloride solution, and the ultrasonic vibration treatment time is not less than 5 min.
5. The animal feed additive according to claim 1, wherein in the step 2), the addition amount and the ratio of the absolute ethyl alcohol, the citric acid, the sodium propionate and the tricalcium phosphate to the solution are 20-30 mL/100mL of the absolute ethyl alcohol, 1-2 g/100mL of the citric acid, 0.3-0.8 g/100mL of the sodium propionate and 0.1-0.2 g/100mL of the tricalcium phosphate, respectively.
6. The animal feed additive according to claim 2, wherein the fresh black plum pulp and the dried jujube are mixed in a mass ratio of black plum pulp: the dry Chinese dates =10: 3-20.
7. The animal feed additive according to claim 2, wherein in the step (2), the addition amount of deionized water is more than 6 times of the total mass of the mixture of the black brin pulp and the dried Chinese dates; the volume percentage content of the ethanol in the ethanol water solution is 60-70 percent, the balance is water, and the added mass of the ethanol water solution is 1/3 of the mass of the liquid phase after the pressure concentration.
8. A feed comprising the feed additive according to any one of claims 1 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911033641.6A CN110771746A (en) | 2019-10-28 | 2019-10-28 | Animal feed additive and feed containing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911033641.6A CN110771746A (en) | 2019-10-28 | 2019-10-28 | Animal feed additive and feed containing same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110771746A true CN110771746A (en) | 2020-02-11 |
Family
ID=69387309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911033641.6A Pending CN110771746A (en) | 2019-10-28 | 2019-10-28 | Animal feed additive and feed containing same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110771746A (en) |
Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004080210A1 (en) * | 2003-03-14 | 2004-09-23 | Boehringer Ingelheim Danmark A/S | Compositions of dietary metals for support of the physiological intestinal development and prevention of diarrhoea |
| CN101139299A (en) * | 2007-09-06 | 2008-03-12 | 广州天科生物科技有限公司 | Method for preparing copper zinc aminoacetic acid complex by ball milling solid state |
| CN102084937A (en) * | 2010-05-24 | 2011-06-08 | 浙江林学院 | Fodder acidulant and application method thereof |
| CN103355558A (en) * | 2013-08-07 | 2013-10-23 | 奥格生物技术(上海)有限公司 | Premix and piglet complete feed prepared from same |
| CN104041675A (en) * | 2014-06-25 | 2014-09-17 | 福建深纳生物工程有限公司 | Simple preparation method for amino acid microelement chelate |
| CN104171702A (en) * | 2014-08-11 | 2014-12-03 | 成都巨星农牧科技有限公司 | Pig feed capable of preventing diarrhea and promoting digestion |
| CN104187082A (en) * | 2014-08-06 | 2014-12-10 | 成都巨星农牧科技有限公司 | Diarrhea-preventive piglet feed |
| CN104222693A (en) * | 2014-10-11 | 2014-12-24 | 来光业 | Pig feed and producing method thereof |
| CN104256096A (en) * | 2014-10-08 | 2015-01-07 | 青岛农业大学 | Feed additive and feed for treating piglet diarrhea |
| CN104489408A (en) * | 2014-12-30 | 2015-04-08 | 瑞金双胞胎饲料有限公司 | Porcine feed additive for effectively improving intestinal health and enhancing immunity |
| CN105732129A (en) * | 2016-02-25 | 2016-07-06 | 河南力浮科技有限公司 | Production method for nanometer humic acid chelated zinc fertilizer |
| CN106359978A (en) * | 2016-08-29 | 2017-02-01 | 广西商大科技股份有限公司 | Nutritive premixing agent for weaned pigs, in place of antibiotic for reducing diarrhoea of weaned pigs |
| CN107821794A (en) * | 2017-10-31 | 2018-03-23 | 广州骊加生物科技有限公司 | A kind of feed addictive for preventing animal diarrhea |
| CN108077598A (en) * | 2017-11-15 | 2018-05-29 | 浙江万方生物科技有限公司 | A kind of amino acid composite trace element chelate and preparation method thereof |
| CN108850593A (en) * | 2018-06-13 | 2018-11-23 | 合肥云都棉花有限公司 | A kind of feed and preparation method thereof with treatment diarrhea of pigs function |
| CN109170285A (en) * | 2018-10-31 | 2019-01-11 | 福建傲农生物科技集团股份有限公司 | A kind of feed addictive and the preparation method and application thereof substituting zinc oxide |
| CN109497308A (en) * | 2018-11-07 | 2019-03-22 | 淮北佳羊生物科技有限公司 | Piglet anti diar rhea additive for microbe feedstuff and preparation method thereof |
| CN110250355A (en) * | 2019-07-04 | 2019-09-20 | 山东畜牧兽医职业学院 | A kind of small powder and its feed of the pre- anti-diarrhea of child care pig |
-
2019
- 2019-10-28 CN CN201911033641.6A patent/CN110771746A/en active Pending
Patent Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004080210A1 (en) * | 2003-03-14 | 2004-09-23 | Boehringer Ingelheim Danmark A/S | Compositions of dietary metals for support of the physiological intestinal development and prevention of diarrhoea |
| CN101139299A (en) * | 2007-09-06 | 2008-03-12 | 广州天科生物科技有限公司 | Method for preparing copper zinc aminoacetic acid complex by ball milling solid state |
| CN102084937A (en) * | 2010-05-24 | 2011-06-08 | 浙江林学院 | Fodder acidulant and application method thereof |
| CN103355558A (en) * | 2013-08-07 | 2013-10-23 | 奥格生物技术(上海)有限公司 | Premix and piglet complete feed prepared from same |
| CN104041675A (en) * | 2014-06-25 | 2014-09-17 | 福建深纳生物工程有限公司 | Simple preparation method for amino acid microelement chelate |
| CN104187082A (en) * | 2014-08-06 | 2014-12-10 | 成都巨星农牧科技有限公司 | Diarrhea-preventive piglet feed |
| CN104171702A (en) * | 2014-08-11 | 2014-12-03 | 成都巨星农牧科技有限公司 | Pig feed capable of preventing diarrhea and promoting digestion |
| CN104256096A (en) * | 2014-10-08 | 2015-01-07 | 青岛农业大学 | Feed additive and feed for treating piglet diarrhea |
| CN104222693A (en) * | 2014-10-11 | 2014-12-24 | 来光业 | Pig feed and producing method thereof |
| CN104489408A (en) * | 2014-12-30 | 2015-04-08 | 瑞金双胞胎饲料有限公司 | Porcine feed additive for effectively improving intestinal health and enhancing immunity |
| CN105732129A (en) * | 2016-02-25 | 2016-07-06 | 河南力浮科技有限公司 | Production method for nanometer humic acid chelated zinc fertilizer |
| CN106359978A (en) * | 2016-08-29 | 2017-02-01 | 广西商大科技股份有限公司 | Nutritive premixing agent for weaned pigs, in place of antibiotic for reducing diarrhoea of weaned pigs |
| CN107821794A (en) * | 2017-10-31 | 2018-03-23 | 广州骊加生物科技有限公司 | A kind of feed addictive for preventing animal diarrhea |
| CN108077598A (en) * | 2017-11-15 | 2018-05-29 | 浙江万方生物科技有限公司 | A kind of amino acid composite trace element chelate and preparation method thereof |
| CN108850593A (en) * | 2018-06-13 | 2018-11-23 | 合肥云都棉花有限公司 | A kind of feed and preparation method thereof with treatment diarrhea of pigs function |
| CN109170285A (en) * | 2018-10-31 | 2019-01-11 | 福建傲农生物科技集团股份有限公司 | A kind of feed addictive and the preparation method and application thereof substituting zinc oxide |
| CN109497308A (en) * | 2018-11-07 | 2019-03-22 | 淮北佳羊生物科技有限公司 | Piglet anti diar rhea additive for microbe feedstuff and preparation method thereof |
| CN110250355A (en) * | 2019-07-04 | 2019-09-20 | 山东畜牧兽医职业学院 | A kind of small powder and its feed of the pre- anti-diarrhea of child care pig |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1806982B1 (en) | Compositions and methods for promoting weight gain and feed conversion | |
| CN112753893A (en) | Preparation method and formula of aquatic feed | |
| CS237345B2 (en) | Method of filling of fodder with l-lysine | |
| CN109043126A (en) | A kind of big dragon shrimp feed and preparation method thereof | |
| FI82178B (en) | PROTEINHALTIG FODERKOMPOSITION FOER OEKNING AV KOETT- ELLER MJOELKPRODUKTIONEN HOS BOSKAP OCH FOERFARANDE FOER UTFODRING AV BOSKAP MED SAODAN FODERKOMPOSITION. | |
| CN110771746A (en) | Animal feed additive and feed containing same | |
| CN111317070A (en) | Feed additive for improving meat quality of fattening pigs and application | |
| RU2284703C1 (en) | Pig feeding method | |
| CN106306480B (en) | Additive comprising sodium glutamate | |
| CS201006B2 (en) | Fodder and admixture in the fodder | |
| CN112970941A (en) | Feeding-attractant glycolysis molasses feed and preparation method thereof | |
| CN113729124A (en) | Compound plant extract, preparation method and application thereof in feed | |
| RU2461211C2 (en) | Energy-sugar-protein concentrate and its production method | |
| RU2259782C2 (en) | Feedstuff for piglets | |
| CN111990535A (en) | Defatted tenebrio molitor protein feed rich in multiple trace elements and preparation method thereof | |
| CN106333082B (en) | Preparation method of amino acid compound feed additive | |
| RU2321268C2 (en) | Fodder for broiler chickens | |
| CN112205538B (en) | Compound feed additive for preventing occurrence of fatty liver bleeding plaques of Langder geese | |
| CN109497338A (en) | Muscovy duck feed and preparation method thereof | |
| Linerode et al. | Effects of Antibiotics, Amino Acids, Zinc and Enzymes on the Growth Depression in the Turkey Produced by Raw Soybeans | |
| CA2406112A1 (en) | Dephytinization of plant based products in mixtures with high moisture animal, plant or microbial by-products | |
| CN112640996B (en) | Nutritional additive for improving pork quality | |
| RU2729387C1 (en) | Fodder additive for young sheep | |
| CN106359896B (en) | Preparation process of additive containing sodium glutamate | |
| CN109393195B (en) | Choline chloride feed additive and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200211 |