CN110742914B - Traditional Chinese medicine composition and application thereof in treating white scour of piglets - Google Patents
Traditional Chinese medicine composition and application thereof in treating white scour of piglets Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition and application thereof in treating white scour of piglets, belonging to the technical field of traditional Chinese medicines for treating diarrhea and diarrhea of piglets, and comprising 700-950 parts by weight of pomegranate rind and 5-300 parts by weight of talc. The traditional Chinese medicine composition is in the form of traditional Chinese medicine powder, and is administrated by drenching, wherein the weight of each 1kg piglet is 0.09g, and the traditional Chinese medicine composition is continuously used for 5 days. The medicine can effectively treat white scour of piglets, the cure rate is 95.38%, the effective rate is 98.46%, the cure rate is 89.66%, and the effective rate is 98.28%, but the symptoms are quickly relieved or disappeared in the medication process, and the symptom values of 2, 3 and 4 days of medication are extremely lower than that of the white Longsan group (P is less than 0.01); the average treatment course is only 2.65 days, and the treatment course is shorter than that of the Bailong powder group.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition and application of the composition in treating white scour of piglets. Belongs to the technical field of traditional Chinese medicine formula and animal disease treatment.
Background
White Scour of Piglets (White school of Piglets) is a non-septic and acute intestinal infectious disease of 10-30 days old Piglets caused by pathogenic escherichia coli. The clinical manifestations are milky white or grey white thin feces, pasty feces and sticky and greasy fishy smell. The sick pigs were severely dehydrated and the mucous membranes were pale. The disease has wide prevalence and rapid spread, the incidence rate can reach 80 percent, and the disease has a trend of obvious rising. If the treatment is not timely or improper, the growth and development of piglets are affected by mild patients, and the stiff pigs are caused by severe patients, so that great economic loss is caused to the pig industry. The disease can be attacked all the year round, wherein early spring, severe winter and hot summer are common.
At present, a plurality of important problems to be solved urgently exist in the prevention and treatment of white scour of piglets: firstly, the serotype of colibacillus causing white scour of piglets is various, and 171 kinds of colibacillus O antigens, 103 kinds of K antigens and 60 kinds of H antigens which are determined so far are provided. The pathogenic serotypes in different regions and different pig farms are greatly different, and the serotypes are constantly changing. Secondly, because of the use of a large amount of antibiotics and chemical antibacterial drugs, the escherichia coli generates broad-spectrum drug resistance, the effectiveness of drug therapy is reduced, the dosage has to be increased clinically and the administration time is prolonged, and the treatment difficulty and the cost are greatly improved. The fan national swallow and the like monitor the drug resistance of 45 strains of escherichia coli separated from 5 large-scale pig farms in Henan province, the 45 strains of escherichia coli have the drug resistance phenomenon to 15 antibacterial drugs, which account for 83% of the 18 selected antibacterial drugs, wherein the drug resistance to tetracycline, compound sulfamethoxazole, florfenicol, norfloxacin and the like is very strong, the drug resistance to streptomycin, doxycycline, erythromycin, ciprofloxacin and the like is high, the multi-drug resistance is shown, the resistance is at least 3, and the resistance is at most 14. 86 escherichia coli are separated from 185 pig disease materials died of illness, the bacterial strain resistant to ciprofloxacin, doxycycline, amoxicillin, tetracycline and sulfamethoxazole in the escherichia coli is as high as more than 80%, 67 bacterial strains show multiple drug resistance, and 53.7% bacterial strains are resistant to more than 9 drugs. Wangmeji et al monitored the clinical separation of pathogenic bacteria drug resistance, found that Escherichia coli to fluoroquinolone antibacterial drugs present higher drug resistance rate, and with other antibacterial drugs have cross drug resistance. Thirdly, antibiotics and chemical drugs are frequently used, which causes serious environmental pollution and endangers public health safety. If a large amount of antibiotics and chemical antibacterial drugs are used for a long time, the excrement of the pigs pollutes soil and water sources, and further pollutes grains, fruits, vegetables and aquatic organisms. The key laboratory of the department of public health and safety education of the university of Compound Dan and the young researchers of the public health academy of the university of Compound Dan, Xinghe, Wanna, etc. collect urine samples of children in the Shanghai region of Jiang, through more than 3 years of research, it is found that the exposure of antibiotics in the period of children may be one of the risk factors of obesity of children, and the corresponding achievements have been published in International Authority journal of environmental field, environmental International (environmental International). In order to research the influence of antibiotic use in children or food antibiotic exposure on lipogenesis of children, a subject group internationally adopts an antibiotic biological monitoring mode for the first time to research 586 name urine samples of 8-11 years old school-age children collected in 2013 in Shanghai, and the result shows that the urine contains 21 antibiotics including 5 macrolides, 2 beta-lactams, 3 tetracyclines, 4 quinolones, 4 sulfonamides and 3 chloramphenics, wherein one or more of the 21 antibiotics are detected in 79.6% of the school-age children urine.
In summary, various measures for treating animal diarrhea at present have respective defects and shortcomings, so that a traditional Chinese veterinary medicine of pomegranate peel antidiarrheal powder for treating white scour of piglets is researched and developed from Chinese medical treasury-traditional Chinese medicine, and a new veterinary medicine certificate is about to be obtained currently in new drug review of national agriculture and rural ministry.
Disclosure of Invention
A traditional Chinese medicine composition and an application thereof in treating white scour of piglets belong to the technical field of traditional Chinese medicines for treating diarrhea and diarrhea of piglets, and comprise 700-950 parts by weight of pomegranate rind and 50-300 parts by weight of talc. The traditional Chinese medicine composition is in the form of traditional Chinese medicine powder, and is administrated by drenching, wherein the weight of each 1kg piglet is 0.09g, and the traditional Chinese medicine composition is continuously used for 5 days. The medicine can effectively treat white scour of piglets, the cure rate is 95.38%, the effective rate is 98.46%, the cure rate is 89.66%, and the effective rate is 98.28%, but the symptoms are quickly relieved or disappeared in the medication process, and the symptom values of 2, 3 and 4 days of medication are extremely lower than that of the white Longsan group (P is less than 0.01); the average treatment course is only 2.65 days, and the treatment course is shorter than that of the Bailong powder group.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the pericarpium granati antidiarrheal powder consists of two medicinal materials of pericarpium granati and talcum, wherein the medical theory is based on the sourness, astringency and warmness of the pericarpium granati. It enters large intestine meridian. Has the effects of astringing intestine to stop diarrhea, stopping bleeding and expelling parasites, and is used for treating chronic diarrhea, chronic dysentery, hematochezia, rectocele, leukorrhagia, abdominal pain due to parasitic infestation. Pomegranate rind contains a large amount of phenolic acid substances such as tannic acid, has a astringing and sterilizing effect on intestinal pathogenic bacteria such as escherichia coli, proteus, typhoid bacillus, paratyphoid bacillus, pseudomonas aeruginosa, vibrio cholerae and the like, can astringe intestines to stop bleeding, and can treat diseases such as dysentery, diarrhea, hematochezia and the like. Talc is a silicate mineral, sweet, bland and cold. It enters bladder, lung and stomach meridians. Has the effects of inducing diuresis for treating stranguria, clearing away summer-heat. Can be used for treating pyretic stranguria, urolithiasis, odynuria, summer-heat dampness polydipsia, damp-heat watery diarrhea; it can be used for treating eczema, and miliaria. The talcum powder mainly contains hydrous magnesium silicate, has large body surface area after being crushed, is distributed with countless tiny cavities, can play a role in adsorbing bacterial toxins and the like and protecting intestinal mucosa, and can be externally used for eliminating dampness and healing sores.
The pomegranate bark and the talc are compatible, so that the monarch drug pomegranate bark can sterilize, astringe intestines and stop bleeding, and meanwhile, the talc can assist the monarch drug to further adsorb residual bacterial toxin and the like in large intestines to cover the surfaces of intestinal mucosa, protect the injured intestinal mucosa and enable the intestinal mucosa to recover as soon as possible.
The traditional Chinese medicine composition comprises, by weight, 700-950 parts of pomegranate bark and 50-300 parts of talc.
The traditional Chinese medicine composition is also preferably 850 parts by weight of pomegranate bark and 150 parts by weight of talc.
The traditional Chinese medicine composition is also preferably 950 parts of pomegranate rind and 50 parts of talcum according to parts by weight.
The traditional Chinese medicine composition is also preferably 900 parts of pomegranate rind and 100 parts of talcum according to parts by weight.
The traditional Chinese medicine composition is also preferably 800 parts of pomegranate bark and 200 parts of talcum according to parts by weight.
The traditional Chinese medicine composition is prepared from, by weight, 700 parts of pomegranate bark and 300 parts of talcum preferentially.
The preparation process of the powder of the traditional Chinese medicine composition comprises the following steps: pulverizing pericarpium Granati according to weight ratio
24 mesh sieve; pulverizing pulvis Talci, and sieving with 100 mesh sieve; mixing pericarpium Granati powder and pulvis Talci, and packaging.
The preparation process of the granules of the traditional Chinese medicine composition comprises the following steps: according to the weight part ratio of the composition, 1/2 parts of pomegranate rind is crushed and sieved by a 24-mesh sieve for later use; pulverizing pulvis Talci, and sieving with 100 mesh sieve; extracting the rest 1/2 pericarpium Granati with 40% ethanol under reflux for 2 times, each for 2 hr, recovering ethanol, concentrating at 70 deg.C under reduced pressure to obtain fluid extract with relative density of 1.25, adding the pericarpium Granati powder, pulvis Talci and dextrin, mixing, granulating with ethanol of appropriate concentration, drying below 70 deg.C, and making into granule 1000 g.
The preparation process of the oral liquid of the traditional Chinese medicine composition comprises the following steps: taking pomegranate rind according to the weight part ratio of the composition, crushing the pomegranate rind into a size suitable for extraction, crushing talc into small blocks, adding water, decocting for 2 times, each time for 2 hours, filtering, concentrating the filtrate at 70 ℃ under reduced pressure to obtain clear paste with the relative density of 1.05, refrigerating and standing for 24 hours, filtering, diluting the filtrate with distilled water until each 1ml of the filtrate equivalently contains 0.5g of raw medicinal materials, filling and sterilizing to obtain the composition.
The traditional Chinese medicine composition with the 3 dosage forms has good treatment effect on diarrhea and diarrhea of piglets, and the medicine effect, the toxicology and the clinical test are preferably carried out by using powder:
pharmacodynamic test
According to the effects of astringing intestines and checking diarrhea, stopping bleeding, expelling parasites and the like, the pomegranate rind is clinically used for treating diseases such as diarrhea and dysentery, hematochezia, rectocele, parasitic amasis and the like. Tests such as bacteriostasis, drug resistance, inhibition of virulence gene expression, antitoxin, regulation of intestinal motility and the like are designed, and the results show that:
1. the pericarpium Granati antidiarrheal powder is prepared into water decoction, and is subjected to in vitro antibacterial test by adopting a flat plate method. The result shows that the water decoction holes of the pomegranate bark antidiarrheal powder have obvious bacteriostasis circles, and the diameter of each bacteriostasis circle is 14.5-15.0 mm; no inhibition zone is formed around the control hole of the normal saline, which shows that the water decoction of the pomegranate rind antidiarrheal powder containing 1g of crude drug per 1ml has the inhibition effect on escherichia coli (a standard strain CVCC196, and separated strains 001 and 002) in vitro.
2. The minimum inhibitory concentration of the water decoction of the pomegranate bark antidiarrheal powder to escherichia coli is proved by a test tube dilution method on the basis that the water decoction of the pomegranate bark antidiarrheal powder has an inhibitory effect by a flat plate method test, the water decoction of the pomegranate bark antidiarrheal powder has an inhibitory effect on escherichia coli CVCC196 and two clinically separated escherichia coli, and the Minimum Inhibitory Concentration (MIC) is 25 mg/ml.
3. The influence of the pomegranate rind antidiarrheal powder on the drug resistance of escherichia coli is proved by adopting clinically separated escherichia coli 001 strain (with strong drug resistance to amoxicillin and ciprofloxacin, MIC is respectively 200 mug/ml and 50 mug/ml), and elimination rates of the clinical separated escherichia coli 001 strain to the amoxicillin drug resistance are respectively 14.0%, 2.0% and 0.4% after 1/2MIC (12.5mg/ml), 1/4MIC (6.25mg/ml) and 1/8MIC (3.13mg/ml) and elimination rates of the clinical separated pomegranate rind antidiarrheal powder to the ciprofloxacin drug resistance are respectively 8.6%, 1.2% and 0.2%, and gel nucleic acid electrophoresis detection is adopted after plasmids are extracted to prove that the pomegranate rind antidiarrheal water decoction has a certain elimination effect on escherichia coli plasmids.
4. In order to observe the influence of the pomegranate rind antidiarrheal powder on the expression of the virulence genes of the escherichia coli, the experiment compares the change condition of the expression level of the virulence related gene mRNA of each drug dose group compared with a blank control group and a negative control group by extracting the total RNA of the escherichia coli before and after the administration of the pomegranate rind antidiarrheal powder and analyzing by a PCR method. The results show that the mRNA expression levels of LT genes in the dose groups in the water decoction of the pomegranate bark antidiarrheal powder are obviously lower than those of the other 3 groups, and the mRNA expression levels of K88 and STb genes in each drug dose group are not obviously different from those of a blank control group and a negative control group. Namely 1/2MIC (12.5mg/ml) and 1/4MIC (6.25mg/ml), the pomegranate bark antidiarrheal water decoction can reduce the mRNA expression level of LT virulence gene of Escherichia coli CVCC 196.
5. In order to observe the influence of the pericarpium granati antidiarrheal water decoction on the intestinal movement of the mice, the water decoction of the pericarpium granati antidiarrheal water decoction with the dosage of 0.5g/kg of body weight, 1.0g/kg of body weight and 2.0g/kg of body weight is respectively used for gastric irrigation of normal mice and senna diarrheal mice, the negative control group of mice is subjected to gastric irrigation with physiological saline with the same volume, and the influence of the negative control group of mice on the intestinal propulsion movement of the normal mice and the senna diarrheal mice is observed and compared. The result shows that the pomegranate bark antidiarrheal water decoction has no obvious influence on the intestinal tract carbon powder propulsion rate of normal mice; compared with the negative control group, the water decoction of the senna leaf diarrhea-inducing mice and the pomegranate bark diarrhea-relieving bulk water decoction (1.0g/kg body weight and 2.0g/kg body weight) has the obviously reduced intestinal charcoal powder propulsion rate (P is less than 0.01). The result shows that the pomegranate bark antidiarrheal water decoction has no obvious influence on the intestinal tract carbon powder propulsion rate of normal mice, but can reduce the intestinal tract carbon powder propulsion rate of senna leaf diarrheal mice.
6. In vitro endotoxin resisting experiment is carried out by limulus reagent test tube agglutination reaction, and the antibacterial endotoxin effect of the pomegranate bark antidiarrheal water decoction is discussed. As a result: in a limulus reagent reaction test with the sensitivity of 0.25EU/ml, the pomegranate bark antidiarrheal water decoction has a good antibacterial endotoxin effect after being diluted by 2000 times (equivalent to 0.5mg/ml of original drug), and meanwhile, the pomegranate bark antidiarrheal water decoction can obviously reduce the release of escherichia coli endotoxin caused by amoxicillin.
Second, pharmacological toxicological test
1. Acute toxicity test in order to evaluate the safety of the pericarpium granati antidiarrheal powder, an acute toxicity test is performed on the pericarpium granati antidiarrheal powder. The acute toxicity of the drug to the tested animals is observed by oral administration of 2 experimental animals including rats and mice. As a result: the decoction of pericarpium Granati antidiarrheal powder is administered to large and small mice at 5000mg/kg body weight, and can not cause death of test animal, i.e. LD of pericarpium Granati antidiarrheal powder for oral acute toxicity test of large and small mice50More than 5000mg/kg body weight proves that the preparation is actually nontoxic, and the result shows that the preparation has higher safety.
2. The subchronic toxicity test inspects the subchronic toxicity effect of the pomegranate rind antidiarrheal powder so as to evaluate the safety of clinical medication and provide a basis for clinical tests and application. Randomly dividing 80 Wistar rats into 4 groups, and respectively feeding 1 time of stomach-feeding pomegranate rind antidiarrheal water decoction to the rats with the dosages of 12g/kg body weight, 6g/kg body weight and 3g/kg body weight, 1 time per day, and continuously using for 4 weeks; the control group was gazed with an equal volume of physiological saline and the animals were observed for a detailed toxicity reaction. As a result: the 3 doses of pomegranate rind antidiarrheal powder have no adverse effect on the general health condition, body weight, feed intake, water intake, blood routine and serum biochemical indexes, and the form, tissue structure and organ coefficient of main organs of the tested animal. The result shows that the pomegranate rind antidiarrheal powder within the dosage of 12g/kg body weight has no sub-chronic toxic effect on rats.
Third, clinical trial
1. Target animal safety test 40 piglets were randomly divided into 4 groups, namely healthy control group, 1-fold dose group (0.09 g/kg by body weight), 3-fold dose group (0.27 g/kg by body weight) and 5-fold dose group (0.45 g/kg by body weight), 10 piglets in each group were continuously dosed for 5 days, and the general clinical manifestations, feed intake and weight changes, hematology and serum biochemical indicators, autopsy and histopathological changes of the test pigs were observed in detail after dosing. The results show that: pomegranate rind antidiarrheal powder at 1, 3 and 5 times the clinically recommended reference dose did not cause death and other toxic reactions in the test pigs. The experimental pig has no abnormal mental state, diet and feces, good growth and development, and no significant difference in the number of red blood cells, hemoglobin content, total white blood cells, white blood cell differential count and various serum biochemical indexes compared with the control group. The tissue structure and the morphology of each organ do not show any abnormal pathological changes through the autopsy and the histological observation. The pericarpium granati antidiarrheal powder has higher clinical medication safety for pigs.
2. The clinical preparation test selects 34 pigs (with weight of 5-8.5 kg) with 28 days old weaned healthy piglets to perform the clinical preparation test of the pomegranate rind antidiarrheal powder, wherein 6 pigs are subjected to the regression test, and 14 pigs are divided into 7 groups (a control group and 6 different dose groups) to be used for groping piglets artificially infected by porcine pathogenic escherichia coli and establishing piglet white diarrhea animal modelsThe bacteria attack dosage, the infection way and the environmental conditions lay a foundation for experimental clinical tests; in the other 14 cases, the effective dose range of the pomegranate rind antidiarrheal powder for treating white scour of piglets is searched for 7 groups (blank control group, positive control group and 5 different dose groups). Namely, after a porcine pathogenic escherichia coli O8(CVCC 196) strain is rejuvenated, intraperitoneal injection and oral inoculation are carried out by using different bacterial quantities, and the low-temperature and humid environmental conditions are simulated to ensure that the tested pig is infected and attacked, and the establishment of an animal model of white scour of piglets is confirmed through clinical diagnosis, caesarean test change and laboratory examination; the animal model of white scour of piglets is treated by the pomegranate rind antidiarrheal powder with different dosages, and the effective dosage range of the pomegranate rind antidiarrheal powder for treating white scour of piglets is found by observing the clinical curative effect. The results show that: under the conditions that the house temperature is 18-20 ℃ and the relative humidity is 70-80%, the pig pathogenic escherichia coli O is applied to piglets on the weaning day of 28 days old8(CVCC 196) 3.0×1010The typical white scour model of piglets can be created by the concentration of cfu/mL of the bacteria for the challenge, and the bacteria liquid is injected into the abdominal cavity for 1 mL/head and is orally administrated for 5 mL/head. The pomegranate rind antidiarrheal powder with the dosage of 0.09g/kg, 0.12g/kg and 0.15g/kg has ideal treatment effect on a piglet white diarrhea model, and the reference dosage of the pomegranate rind antidiarrheal powder for treating piglet white diarrhea can be selected within the range of 0.06g/kg, 0.09g/kg and 0.12g/kg in further clinical tests.
3. Clinical trial 72 piglets of 28 days old (5-8.5 kg) were randomly divided into 6 groups (blank control group, positive control group, high, medium and low dose group of pericarpium Granati antidiarrheal powder, Bailongsan group), and each group had 12 piglets, except the blank control group, and reference the prepared clinical trial results to the rest groups, and porcine Escherichia coli standard strain O was prepared8(C83902) At 3.0 × 1010The cfu/mL of the concentration of the bacteria for the attack is injected into the abdominal cavity and inoculated through the mouth, and the conditions of low temperature, humidity and multiple stress are simulated, so that the tested pig is infected and attacked, and the pig is used for clinical test after typical and stable clinical symptoms appear and meet the standard of a piglet white dysentery disease model. The Chinese medicinal preparation, namely the pericarpium granati antidiarrheal powder, is administrated to sick piglets at the dosage of 0.06/kg, 0.09g/kg and 0.12/kg, 1 time per day and 5 days after continuous administration. Selecting BAILONGSAN as control drug, setting positive control group and blank control group, and observing pericarpium Granati antidiarrheal powder in predetermined periodThe treatment effect and the treatment course on the artificial infection of white scour of piglets and the influence on the growth and development conditions of sick pigs. The results show that: injecting standard strain O of swine escherichia coli into abdominal cavity under the premise of existence of multiple stress factors8The strain successfully replicates a white dysentery disease model of piglets, infected piglets show typical and stable clinical symptoms, and the morbidity is 100%. The clinical symptoms of the piglets taking the pomegranate rind antidiarrheal powder with high and medium doses (0.12g/kg and 0.09g/kg) are quickly improved, the piglets are lively, the diet is quickly improved, the defecation is gradually restored to be normal, and the weight gain of the piglets is quickly restored; the high and medium dose groups have better curative effect than the low dose group and the control drug group, the course of treatment is shorter, the piglets treated by the high and medium dose groups can quickly recover the normal growth, and the porcine escherichia coli standard strain O is injected into the abdominal cavity on the premise that multiple stress factors exist8The strain successfully replicates a white dysentery disease model of piglets, infected piglets show typical and stable clinical symptoms, and the morbidity is 100%. 3 days before toxin counteracting, the powder is orally taken according to the dosage of 0.09g pomegranate rind antidiarrheal powder per 1kg body weight, and is used for treating swine escherichia coli standard strain O8The piglet white diarrhea caused by the strain has obvious protective effect, and the protective rate reaches 91.67 percent. The clinical symptoms of the piglets taking the pomegranate rind antidiarrheal powder with high and medium doses (0.12g/kg and 0.09g/kg) are quickly improved and cured, the piglets are lively, the diet is quickly improved, the defecation is gradually restored to be normal, and the weight gain of the piglets is quickly restored; the curative effect of the high and medium dose groups is better than that of the low dose group and the control drug group, the treatment course is shorter, and the piglets treated by the high and medium dose groups can quickly recover the normal growth and development. The results show that: the pomegranate rind antidiarrheal powder with the dosage of 0.12g, 0.09g and 0.06g per 1kg of body weight can be taken orally, and can effectively treat the artificial infection of the swine escherichia coli standard strain O8The cure rate of the white scour of piglets caused by the strain is 83.33%, 75.00% and 50.00%, and the effective rate is 100%. The pomegranate rind antidiarrheal powder (0.12g/kg, 0.09g/kg and 0.06g/kg) is orally taken to treat white scour of piglets caused by artificial infection, the average treatment course is 4.7 days, 4.78 days and 5 days, and the spirits, appetite, fecal characters and body weight gain of sick pigs are quickly recovered. The pomegranate rind antidiarrheal powder has no obvious difference between high and medium dosages (0.12g/kg and 0.09g/kg), and 0.09g/kg body weight is recommended as the clinical medication recommended dose.
4. The result of an early-stage clinical test of an expanded clinical test shows that the pomegranate rind antidiarrheal powder with the weight of 0.09g/kg is administrated every day to have a good treatment effect on white scour of piglets caused by artificial infection, in order to verify the curative effect in a large range, expanded clinical test research is carried out according to the technical guidance principle of veterinary traditional Chinese medicine and natural medicine clinical tests of the veterinary drug review center of the Ministry of agriculture, 30 litters are selected, diagnosis is determined by epidemiological investigation, clinical examination, pathological dissection and laboratory examination, 27 litters and 123 litters which meet the case inclusion standard are randomly divided into 2 litters, 65 littoral antidiarrheal powder treatment groups and 58 Bailongsan control groups are administrated, and the pomegranate rind antidiarrheal powder and the Bailongsan are administrated respectively. The effectiveness and safety of the pomegranate rind antidiarrheal powder for treating white scour of piglets are comprehensively judged by observing the clinical symptoms such as defecation frequency, excrement form, spirit, appetite and the like, and the body weight and the like before administration and after 5 days after the administration. The results show that: the pomegranate rind antidiarrheal powder has better curative effect on white scour of piglets. The clinical symptoms of the piglets taking the pomegranate rind antidiarrheal powder are quickly improved and cured, the spirit is gradually active, the accumulation is gradually reduced, the activity is increased, the mouth and the nose are moist, the diet is quickly improved, the defecation is gradually recovered to be normal, the fur is gradually bright, and the weight gain of the piglets is quickly recovered; compared with the Bailongsan group, the pomegranate bark antidiarrheal powder group has the advantages of quick recovery of symptoms, short treatment course and quick recovery of normal growth of piglets. The results show that: the pomegranate rind antidiarrheal powder is orally taken at 0.09g/kg body weight, and can effectively treat white scour of piglets after being continuously taken for 5 days, the cure rate is 95.38 percent, the effective rate is 98.46 percent, the cure rate is 89.66 percent and the effective rate is 98.28 percent, but the symptoms are quickly relieved or disappeared in the medication process, and the symptom scores of 2, 3 and 4 days of medication are remarkably lower than that of the medicine of the white Longsan (P is less than 0.01); the average treatment course is only 2.65 days, and the treatment course is shorter than that of Bailongsan. Based on the above conclusions, the recommended clinical dose is 0.09g/kg body weight, and the medicine is continuously taken for 3-5 days.
The invention has the following innovation points and beneficial effects:
1. the innovation of the formula and the safety and the effectiveness of the application.
So far, the prescriptions, efficacies or indications of 9 types of pomegranate rind powders are consulted, and the detailed descriptions are as follows:
1) the pomegranate rind powder consists of pomegranate rind, betel nut, rangooncreeper fruit and the like, and has the functions of: it can be used for treating abdominal pain due to parasitic infestation caused by ascaris, pinworm and tapeworm.
2) The pomegranate rind powder comprises fried pomegranate rind, angelica, donkey-hide gelatin, moxa leaf carbon and the like, and has the functions of: astringe to stop bleeding, treat metrorrhagia and metrostaxis and incessant pregnancy bleeding.
3) The five-flavor pomegranate rind powder consists of pomegranate rind, safflower, sharpleaf galangal fruit, cinnamon and long pepper, and has the functions of: warming spleen and stomach.
4) The pomegranate rind powder consists of acid pomegranate rind, dragon bone and myrobalan, and has the main treatment effects that: it is indicated for dysentery with reddish and white discharge.
5) The pomegranate rind powder consists of acid pomegranate rind, donkey-hide gelatin, cortex lycii radicis, golden cypress, Chinese angelica and ligusticum wallichii, and is mainly used for treating the following diseases: it is indicated for dysentery with reddish and white urine during pregnancy.
6) The pomegranate rind powder consists of acid pomegranate rind, dried ginger, coptis root and myrobalan, and has the main treatment effects that: infantile bloody purulent dysentery.
7) The pomegranate peel powder consists of pomegranate peel, bitter orange and angelica and mainly comprises the following components in part by weight: dysentery with bloody stool.
8) The pomegranate peel powder consists of pomegranate peel, dried orange peel, liquorice, angelica sinensis and poppy shell and mainly comprises the following components: can be used for treating dysentery.
9) The seven-ingredient pomegranate rind powder consists of pomegranate rind, Chinese pulsatilla root, folium artemisiae argyi, dried orange peel, scorched hawthorn fruit, liquorice and mung bean, and has the functions of: clear heat and remove toxicity, induce diuresis to alleviate edema, astringe intestines to stop diarrhea.
The invention is composed of two medicinal materials of pomegranate rind and talcum. The functional indications are as follows: astringe intestines to check diarrhea, it is indicated for white scour of piglets.
The prescription and the function of the pomegranate rind powder are different from those of the invention. The functions and the main indications of the invention are obtained through clinical and expanded clinical tests on the basis of pharmacodynamic tests of the pomegranate bark antidiarrheal powder. Has definite function, specific main treatment, reasonable and accurate use amount and safe and high-efficiency treatment effect. The same report has not been found at present. Has innovativeness and clinical practicability.
2. The traditional Chinese medicine composition is only prepared by two medicinal materials in the form of powder of medicinal material powder, and is administrated by drenching, so that the composition is easy to accept by piglets, no loss of the administration dosage can be realized, most of the effective components in the powder are digested and absorbed in the stomach of the piglets, and one part of the effective components is absorbed in the intestinal excretion process, so that the antidiarrheal effect is fully exerted. Meanwhile, the traditional Chinese medicine powder has no loss of effective components, simple preparation process and low production cost, and is suitable for large-scale application in animal husbandry.
3. The treatment of the piglet diarrhea in the nursing period mainly aims at inhibiting virus and repairing intestinal tracts, and only excessive anti-inflammatory drugs are given, so that the intractable diarrhea is repeated! The pomegranate rind antidiarrheal powder can really control the occurrence of diarrhea of the piglets only by effectively promoting the recovery of intestinal mucosa of the piglets while inhibiting and diminishing inflammation, and the pomegranate rind antidiarrheal powder utilizes the effective component tannic acid of the pomegranate rind to inhibit and kill viruses, and simultaneously has talc to assist in absorbing bacterial toxins and the like and protect the intestinal mucosa, thereby realizing the good treatment effects of quickly exerting astringency in the bodies of the piglets and promoting the recovery of the intestinal mucosa.
4. The traditional Chinese medicine composition is taken at a dose of 0.09g/kg, and the daily dose of a piglet of 5-8.5 kg is about equal: 0.45-0.765 g of the medicinal materials are dissolved in 3-5 ml of water and then are drenched. The daily dose is small, the treatment effect is good, in clinical expansion tests, the cure rate reaches 95.38%, and the effective rate reaches 98.46%; the curative ratio is 89.66 percent and the effective rate is 98.28 percent compared with the Bailongsan group, but the symptoms are quickly relieved or disappeared in the medication process, and the symptom scores of 2, 3 and 4 days of medication are extremely lower than that of the Bailongsan group (P is less than 0.01); the average treatment course is only 2.65 days, and the treatment course is shorter than that of Bailongsan. Therefore, the pomegranate rind antidiarrheal powder has better effect in the same variety at present. And further verifies that the Chinese medicine is a great treasure house.
The specific implementation mode of the invention is as follows:
example 1
Prescription: 850 parts of pomegranate bark and 150 parts of talcum
The preparation method comprises the following steps: taking pomegranate rind, crushing and sieving the pomegranate rind by a 24-mesh sieve according to the weight part ratio of the composition; pulverizing pulvis Talci, and sieving with 100 mesh sieve; mixing pericarpium Granati powder and pulvis Talci, and packaging.
Taking the pericarpium Granati antidiarrheal powder at a dose of 0.09g per 1kg piglet according to the weight of the sick piglet, dissolving with appropriate amount of water, and administrating for 5 days. The good effect of curing the white scour of the piglets by 95.0 percent is obtained.
Example 2
Prescription: 850 parts of pomegranate bark and 150 parts of talcum
The preparation method comprises the following steps: taking the pomegranate rind and the talc according to the weight part ratio of the composition, and preparing the granules according to the preparation method of the granules.
Taking the pericarpium Granati antidiarrheal granule at a dose of 0.09g per 1kg piglet according to the weight of the sick piglet, dissolving with appropriate amount of water, and administrating for 5 days. The good effect of curing the white scour of the piglets by 94.6 percent is obtained.
Example 3
Prescription: 850 parts of pomegranate bark and 150 parts of talcum
The preparation method comprises the following steps: taking pomegranate rind and talc according to the weight part ratio of the composition, and preparing the oral liquid according to the preparation method of the oral liquid.
Taking the pomegranate rind antidiarrheal oral liquid according to the weight of the sick piglets and the dosage of 0.09g per 1kg of piglets, and drenching for 5 days continuously. The good effect of curing the white scour of the piglets by 94.8 percent is obtained.
Example 4
Prescription: 950 parts of pomegranate bark and 50 parts of talcum
The preparation method comprises the following steps: taking pomegranate rind, crushing and sieving the pomegranate rind by a 24-mesh sieve according to the weight part ratio of the composition; pulverizing pulvis Talci, and sieving with 100 mesh sieve; mixing pericarpium Granati powder and pulvis Talci, and packaging.
Taking the pericarpium Granati antidiarrheal powder at a dose of 0.09g per 1kg piglet according to the weight of the sick piglet, dissolving with appropriate amount of water, and administrating for 5 days. The good effect of curing the white scour of the piglets by 96.5 percent is obtained.
Example 5
Prescription: 900 parts of pomegranate bark and 100 parts of talcum
The preparation method comprises the following steps: taking pomegranate rind, crushing and sieving the pomegranate rind by a 24-mesh sieve according to the weight part ratio of the composition; pulverizing pulvis Talci, and sieving with 100 mesh sieve; mixing pericarpium Granati powder and pulvis Talci, and packaging.
Taking the pericarpium Granati antidiarrheal powder at a dose of 0.09g per 1kg piglet according to the weight of the sick piglet, dissolving with appropriate amount of water, and administrating for 5 days. The good effect of curing the white scour of the piglets by 95.8 percent is obtained.
Example 6
Prescription: 800 parts of pomegranate peel and 200 parts of talcum
The preparation method comprises the following steps: taking pomegranate rind, crushing and sieving the pomegranate rind by a 24-mesh sieve according to the weight part ratio of the composition; pulverizing pulvis Talci, and sieving with 100 mesh sieve; mixing pericarpium Granati powder and pulvis Talci, and packaging.
Taking the pericarpium Granati antidiarrheal powder at a dose of 0.09g per 1kg piglet according to the weight of the sick piglet, dissolving with appropriate amount of water, and administrating for 5 days. The good effect of curing the white scour of the piglets by 95.2 percent is obtained.
Example 7
Prescription: 700 parts of pomegranate peel and 300 parts of talcum
The preparation method comprises the following steps: taking pomegranate rind, crushing and sieving the pomegranate rind by a 24-mesh sieve according to the weight part ratio of the composition; pulverizing pulvis Talci, and sieving with 100 mesh sieve; mixing pericarpium Granati powder and pulvis Talci, and packaging.
Taking the pericarpium Granati antidiarrheal powder at a dose of 0.09g per 1kg piglet according to the weight of the sick piglet, dissolving with appropriate amount of water, and administrating for 5 days. The good effect of curing the white scour of the piglets by 94.9 percent is obtained.
Claims (8)
1. A traditional Chinese medicine composition for treating white scour of piglets is characterized in that: the pomegranate peel preservative comprises, by weight, 700-950 parts of pomegranate peel and 50-300 parts of talc.
2. The traditional Chinese medicine composition for treating white scour of piglets according to claim 1, which is characterized in that: the pomegranate peel preservative comprises 850 parts of pomegranate peel and 150 parts of talcum according to parts by weight.
3. The traditional Chinese medicine composition for treating white scour of piglets according to claim 1, which is characterized in that: the pomegranate peel preservative comprises 950 parts of pomegranate peel and 50 parts of talcum according to parts by weight.
4. The traditional Chinese medicine composition for treating white scour of piglets according to claim 1, which is characterized in that: the pomegranate peel preservative comprises, by weight, 900 parts of pomegranate peel and 100 parts of talcum.
5. The traditional Chinese medicine composition for treating white scour of piglets according to claim 1, which is characterized in that: the pomegranate peel powder comprises 800 parts of pomegranate peel and 200 parts of talcum according to parts by weight.
6. The traditional Chinese medicine composition for treating white scour of piglets according to claim 1, which is characterized in that: the pomegranate peel powder comprises, by weight, 700 parts of pomegranate peel and 300 parts of talc.
7. The traditional Chinese medicine composition for treating white scour of piglets according to claim 1, which is characterized in that: the traditional Chinese medicine composition is powder, capsules and oral liquid.
8. The use of the Chinese medicinal composition of any one of claims 1-7 in the preparation of a medicament for treating white scour of piglets.
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