CN110710606A - Functional composition and feed for reducing content of trans-fatty acid in ruminant milk and application of functional composition and feed - Google Patents
Functional composition and feed for reducing content of trans-fatty acid in ruminant milk and application of functional composition and feed Download PDFInfo
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Abstract
The invention discloses a functional composition for reducing the content of trans-fatty acid in ruminant milk, a feed and application thereof. The invention firstly discloses a functional composition for reducing the content of trans-fatty acid in ruminant milk, which comprises the following components in percentage by weight: 10-15% of yeast culture, 5-10% of rumen bypass choline, 13-17% of rumen bypass methionine and 60-70% of carrier. The invention further discloses a feed containing the functional composition and application thereof. The yeast culture, the rumen bypass choline and the rumen bypass methionine are added into the feed for reducing the content of the trans-fatty acid in the ruminant milk, and meanwhile, the feed follows the design principle of high NDF content and low starch content, so that the proportion of the trans-fatty acid in the ruminant milk to the total fatty acid under the condition of no silage ration is effectively reduced, the milk quality is improved, and the normal proportion of the milk fat rate and the milk protein rate in the ruminant milk is ensured.
Description
Technical Field
The invention relates to the field of livestock breeding. More particularly, relates to a functional composition for reducing the content of trans-fatty acid in ruminant milk, a feed additive and application thereof.
Background
At present, the dairy goat breeding industry mainly faces the problems that silage is difficult to prepare in individual areas or the groups are too small, so that silage type Total Mixed Ration (TMR) is difficult to prepare, and therefore the dairy goat breeding industry can only feed non-silage type ration. In order to improve milk yield, a high proportion of concentrated feed needs to be fed, and the problem of picky feeding of the dairy goat is solved, so that the proportion of milk fat and milk protein is abnormal, the risk of acidosis is increased, and the trans-fatty acid content in the goat milk is increased (the trans-fatty acid content is higher than 3% of the total fatty acid). The national standard for food safety in infant formula (GB10765-2010) states that the maximum trans fatty acid content should be less than 3% of the total fatty acids. Therefore, the goat milk can not be used for producing infant milk powder, the application value of the goat milk is reduced, and meanwhile, the benefits of dairy farmers are affected.
Therefore, under the condition of no silage type daily ration, a new feed needs to be developed urgently to reduce the content of trans-fatty acid in the goat milk, improve the milk quality and ensure the normal proportion of milk fat and milk protein in the goat milk.
Disclosure of Invention
The first purpose of the invention is to provide a functional composition for reducing the content of trans-fatty acid in ruminant milk and an application thereof in preparing feed for reducing the content of trans-fatty acid in ruminant milk.
The second purpose of the invention is to provide a feed for reducing the content of trans-fatty acid in ruminant milk and a feeding method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention firstly provides a functional composition for reducing the content of trans-fatty acid in ruminant milk.
The functional composition for reducing the content of the trans-fatty acid in the milk of the ruminant comprises the following components in percentage by weight: 10-15% of yeast culture, 5-10% of rumen bypass choline, 13-17% of rumen bypass methionine and 60-70% of carrier.
The functional composition of the present invention may consist of the yeast culture, the rumen bypass choline, the rumen bypass methionine and the carrier in the above weight percentages, and may also comprise other components.
Further, the carrier is a plant feed carrier, and can be corn cob powder.
The invention also provides application of the functional composition for reducing the content of the trans-fatty acid in the ruminant milk in preparing feed for reducing the content of the trans-fatty acid in the ruminant milk.
The invention further provides a feed for reducing the content of trans fatty acids in ruminant milk.
The feed for reducing the content of trans-fatty acid in ruminant milk comprises 0.8-1.5 wt% of the functional composition; preferably 1.0% to 1.5%.
Further, the feed for reducing the content of the trans-fatty acid in the milk of the ruminant also comprises the following components in percentage by weight: 10-35% of corn flour, 5-20% of soybean meal, 0-15% of cottonseed meal, 0-15% of alfalfa meal, 5-18% of corn bran, 10-18% of bran, 8-23% of soybean hull, 0-6% of corn protein powder, 5-15% of wheat middling, 0-5% of apple pomace, 0.5-2% of rumen bypass fat powder and 3-5% of premix.
Further, the premix is a compound premix feed for ewes in the lactation period, and the compound premix feed for the ewes in the lactation period is purchased from the European vitamin Lisheng (Shandong) animal nutrition limited company; the goods number is: 19011101060001, respectively; product code number: 3375.
the feed for reducing the content of the trans-fatty acid in the ruminant milk can be composed of corn flour, bean pulp, cottonseed meal, alfalfa meal, corn bran, bean hull, corn protein powder, wheat middling, apple pomace, rumen bypass fat powder, premix and functional composition in percentage by mass, and can also contain other components. The feed for reducing the content of the trans-fatty acid in the milk of the ruminant is prepared by adopting a conventional method in the prior art; in a specific embodiment of the invention, all the components are mixed and granulated to obtain the compound.
The feed for reducing the content of the trans-fatty acid in the ruminant milk has the Neutral Detergent Fiber (NDF) content of 30-50% and the starch content of 15-25%.
The functional composition for reducing the content of the trans fatty acid in the milk of the ruminant or the feed for reducing the content of the trans fatty acid in the milk of the ruminant are also applied to the following applications:
u1, for use in reducing the proportion of trans fatty acids to total fatty acids in ruminant milk, or for use in preparing a product for reducing the proportion of trans fatty acids to total fatty acids in ruminant milk;
u2, use in improving the milk fat rate in ruminant milk, or, use in the preparation of a product for improving the milk fat rate in ruminant milk;
u3, in improving the milk yield of ruminants, or, in preparing a product for improving the milk yield of ruminants;
u4, use for improving the rumen health of ruminants, or use for the preparation of a product for improving the rumen health of ruminants.
The invention further provides a method for feeding ruminants.
The method for feeding ruminants according to the invention comprises: feeding a ruminant with hay and a feed that reduces the trans fatty acid content of the ruminant milk as a ration; wherein the hay is consumed freely and the feed for reducing the trans-fatty acid content in the milk of ruminants is 1-3kg per animal per day.
Further, the hay is oat hay and/or alfalfa hay.
Furthermore, the hay and the feed for reducing the content of the trans-fatty acid in the ruminant milk are fed in separate tanks, and the feed for reducing the content of the trans-fatty acid in the ruminant milk is fed three times a day.
In the present invention, the ruminant is a sheep, preferably a dairy goat.
The invention has the following beneficial effects:
the feed for reducing the trans-fatty acid content in the ruminant milk comprises a large amount of feed raw materials with high fiber content, and the rumen-bypass fat powder with very high saturation is selected, so that the milk fat rate of the ruminant milk is obviously improved, the intake of unsaturated fatty acid is reduced, the trans-fatty acid content in the ruminant milk is further reduced, the ratio of energy provided by non-carbohydrate of daily ration is increased, and the risk of rumen acidosis is reduced. Further, on the basis of the components with high content of effective fiber, the yeast culture, the rumen bypass choline and the rumen bypass methionine are added, so that the proportion of trans fatty acid in the ruminant milk to total fatty acid under the condition of no silage ration can be effectively reduced, the milk quality is improved, and the normal proportion of the milk fat rate and the milk protein rate in the ruminant milk is ensured. In addition, the feed amount of the feed for reducing the trans-fatty acid content in the milk of the ruminant is kept at 1-3 kg/day/feed, the feed amount of the feed can be adjusted according to the weight, the milk yield and the lactation stage so as to adjust the nutrition level of the daily ration, the problem of low fiber intake caused by sheep picky is solved, the nutritional requirement of the ruminant is met, and the rumen health degree is improved.
Detailed Description
In order to more clearly illustrate the invention, the invention is further described below in connection with preferred embodiments. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.
In a first aspect, the invention provides a functional composition for reducing the content of trans fatty acid in ruminant milk, which comprises the following components in percentage by weight: yeast cultures 10% -15% (e.g., can be 10%, 12%, 15%, etc. or any range between any weight percentages), ruminal bypass choline 5% -10% (e.g., can be 5%, 8%, 10%, etc. or any range between any weight percentages), ruminal bypass methionine 13% -17% (e.g., can be 13%, 14%, 15%, 16%, 17%, etc. or any range between any weight percentages), and carriers 60% -70% (e.g., can be 60%, 65%, 70%, etc. or any range between any weight percentages).
The functional composition of the present invention may consist of the yeast culture, the rumen bypass choline, the rumen bypass methionine and the carrier in the above weight percentages, and may also comprise other components.
In particular, the carrier is a plant feed carrier, such as corncob meal.
In a second aspect, the invention provides the use of the functional composition for reducing the content of trans fatty acid in ruminant milk in the preparation of a feed for reducing the content of trans fatty acid in ruminant milk.
In a third aspect, the present invention provides a feed for reducing the trans fatty acid content in ruminant milk, comprising 0.8% to 1.5% by weight (e.g., may be 0.8%, 1.0%, 1.2%, 1.3%, 1.5%, etc., or any range therebetween); preferably 1.0% to 1.5%.
The functional composition of the present invention comprises yeast culture (trade name: Yikang XPC; manufacturer: Danongwei USA; product number: L/2019-05-23/PASS), rumen bypass choline (trade name: Ecolin; manufacturer: Kande; product number: 190726), and rumen bypass methionine (trade name:
the manufacturer: win Chuangdegusai; the goods number is: 270818-26), regulating rumen flora structure by yeast culture to make rumen microorganism proceed unsaturated fatty acid hydrogenation product to saturated fatty acid instead of producing trans fatty acid, and enhancing liver function by rumen bypass choline and rumen bypass methionine, optimizing liver fat metabolite, and further reducing trans fatty acid production in milk.
Specifically, the feed for reducing the content of the trans-fatty acid in the milk of the ruminant also comprises the following components in percentage by weight: corn meal 10-35% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, etc., or any range between any weight percentages), soybean meal 5-20% (e.g., 5%, 10%, 15%, 20%, etc., or any range between any weight percentages), cottonseed meal 0-15% (e.g., 0%, 3%, 5%, 8%, 10%, 12%, 15%, etc., or any range between any weight percentages), alfalfa meal 0-15% (e.g., 0%, 3%, 5%, 8%, 10%, 12%, 15%, etc., or any range between any weight percentages), corn bran 5-18% (e.g., 5%, 8%, 10%, 12%, 15%, 18%, etc., or any range between any weight percentages), wheat bran 10-18% (e.g., 10%, 35%, 12%, 15%, 18%, etc. or any range between any weight percent), 8-23% (e.g., may be 8%, 10%, 12%, 15%, 18%, 20%, 23%, etc. or any range between any weight percent), 0-6% (e.g., may be 0%, 2%, 4%, 6%, etc. or any range between any weight percent), 5-15% (e.g., may be 5%, 10%, 15%, etc. or any range between any weight percent), 0-5% (e.g., may be 0%, 1%, 2%, 3%, 4%, 5%, etc. or any range between any weight percent), 0.5-2% (e.g., may be 0.5%, 0.8%, 1%, 1.2%, 1.5%, 2%, etc. or any range between any weight percent), and 3.0% -5.0% (e.g., may be 0.5%, 0.8%, 1%, 1.2%, 1.5%, 2%, etc.) and 3.0% -5.0% (e.g., may be any range between any weight percent) of a 3.0%, 3.5%, 4.0%, 4.5%, 5%, etc., or any range between any weight percentages).
Specifically, the premix is a compound premix feed for ewes in lactation period (manufacturer: Ouyi Lisheng (Shandong) animal nutrition Co., Ltd.; product number: 19011101060001; product code number: 3375).
The feed for reducing the content of the trans-fatty acid in the ruminant milk can be composed of corn flour, bean pulp, cottonseed meal, alfalfa meal, corn bran, bean hull, corn protein powder, wheat middling, apple pomace, rumen bypass fat powder, premix and functional composition in percentage by mass, and can also contain other components. The feed for reducing the content of the trans-fatty acid in the milk of the ruminant is prepared by adopting a conventional method in the prior art; in a specific embodiment of the invention, all the components are mixed and granulated to obtain the compound.
The feed for reducing the trans-fatty acid content in the ruminant milk contains a large amount of feed raw materials with high fiber content, and rumen-bypass fat powder (trade name: Muyueyou, manufacturer: inner Mongolia Muyuanquanxing feed Limited liability company; product number: 190823F1272167) with very high saturation is selected, so that the milk fat rate of the ruminant milk is remarkably improved, the intake of unsaturated fatty acid is reduced, the trans-fatty acid content in the ruminant milk is further reduced, and the risk of rumen acidosis is reduced.
The feed for reducing the content of the trans-fatty acid in the ruminant milk has the Neutral Detergent Fiber (NDF) content of 30-50% and the starch content of 15-25%.
The functional composition for reducing the content of the trans fatty acid in the milk of the ruminant or the feed for reducing the content of the trans fatty acid in the milk of the ruminant are also applied to the following applications:
u1, for use in reducing the proportion of trans fatty acids to total fatty acids in ruminant milk, or for use in preparing a product for reducing the proportion of trans fatty acids to total fatty acids in ruminant milk;
u2, use in improving the milk fat rate in ruminant milk, or, use in the preparation of a product for improving the milk fat rate in ruminant milk;
u3, in improving the milk yield of ruminants, or, in preparing a product for improving the milk yield of ruminants;
u4, use for improving the rumen health of ruminants, or use for the preparation of a product for improving the rumen health of ruminants.
In a fourth aspect, the present invention provides a method of feeding a ruminant animal comprising: feeding a ruminant with hay and a feed that reduces the trans fatty acid content of the ruminant milk as a ration; wherein the hay is consumed freely and the feed for reducing the trans-fatty acid content in the milk of ruminants is 1-3kg per animal per day.
Specifically, the hay is oat hay and/or alfalfa hay.
Specifically, the hay and the feed for reducing the content of the trans-fatty acid in the ruminant milk are fed in separate tanks, and the feed for reducing the content of the trans-fatty acid in the ruminant milk is fed three times a day.
In the present invention, the ruminant is a sheep, preferably a dairy goat.
The feed amount of the feed for reducing the trans-fatty acid content in the milk of the ruminant is kept at 1-3 kg/day/feed, the feed amount of the feed can be adjusted according to the weight, the milk yield and the lactation stage so as to adjust the nutrition level of the daily ration, the problem of low fiber intake caused by sheep picky feeding is solved, the nutritional requirement of the ruminant is met, and the rumen health degree is improved.
The present invention will be further illustrated by the following specific examples.
The test methods used in the following examples are all conventional methods unless otherwise specified; reagents, biomaterials, etc. used in the following examples are commercially available unless otherwise specified. The equipment is conventional in the technical field.
Trade names, manufacturers and product numbers of the components in the following examples:
rumen bypass fat powder: trade name: grazing Yuan and high-quality; the manufacturer: inner mongolia grazing quanxing fodder llc; the goods number is: 190823F 1272167.
Yeast culture: trade name: yikang XPC; the manufacturer: darnongwei, usa; the goods number is: L/2019-05-23/PASS.
Rumen bypass choline: trade name: (ii) an echolin; the manufacturer: kangde rights; the goods number is: 190726.
rumen bypass methionine: trade name:
the manufacturer: win Chuangdegusai; the goods number is: 270818-26.
Premixing: trade name: the compound premix feed for ewes in lactation period is prepared from the following components in percentage by weight: oryzanol (Shandong) animal Nutrition, Inc.; the goods number is: 19011101060001, respectively; product code number: 3375.
example 1A functional composition for reducing the trans fatty acid content in ruminant milk
Mixing yeast culture 10kg, rumen bypass choline 10kg, rumen bypass methionine 17kg and corncob meal 63kg uniformly to obtain the functional composition for reducing the trans-fatty acid content in ruminant milk.
Example 2A functional composition for reducing the trans fatty acid content in ruminant milk
Mixing yeast culture 15kg, rumen bypass choline 10kg, rumen bypass methionine 13kg and corn cob powder 62kg uniformly to obtain the functional composition for reducing the trans-fatty acid content in ruminant milk.
Example 3A functional composition for reducing the trans fatty acid content in ruminant milk
And uniformly mixing 12.5kg of yeast culture, 7.5kg of rumen bypass choline, 15kg of rumen bypass methionine and 65kg of corncob meal to obtain the functional composition for reducing the content of trans-fatty acid in the ruminant milk.
Example 4A feed for reducing the trans-fatty acid content of ruminant milk
30kg of corn flour, 8kg of soybean meal, 10kg of bran, 8kg of cottonseed meal, 10kg of corn bran, 14kg of soybean hull, 14kg of wheat middling, 0.8kg of rumen bypass fat powder, 4.2kg of premix and 1kg of functional composition (wherein the mixture ratio of the components is 12.5% of yeast culture, 7.5% of rumen bypass choline, 15% of rumen bypass methionine, 65% of corn cob powder and the mass percentage of the components) are mixed and granulated, and the feed for reducing the content of trans-fatty acid in the milk of the ruminant is obtained.
Example 5A feed for reducing the trans-fatty acid content of ruminant milk
Mixing 28kg of corn flour, 14kg of soybean meal, 10kg of bran, 8kg of alfalfa meal, 9kg of corn bran, 12.5kg of soybean hull, 12kg of wheat middling, 1.5kg of rumen bypass fat, 3.5kg of premix and 1.5kg of functional composition (wherein the ratio of the components is 10% of yeast culture, 10% of rumen bypass choline, 17% of rumen bypass methionine and 63% of corn cob powder), and granulating to obtain the feed for reducing the content of trans-fatty acid in the ruminant milk.
Example 6A feed for reducing the trans-fatty acid content of ruminant milk
Mixing and granulating 17kg of corn flour, 17.5kg of soybean meal, 17kg of corn bran, 16kg of bran, 8kg of soybean hull, 2.7kg of alfalfa meal, 3kg of corn protein powder, 7kg of wheat middling, 5kg of apple pomace, 1.2kg of rumen bypass fat, 4.8kg of premix and 0.8kg of functional composition (wherein the ratio of the components is 15% of yeast culture, 10% of rumen bypass choline, 13% of rumen bypass methionine and 62% of corn cob meal), and obtaining the feed for reducing the content of trans-fatty acid in the milk of the ruminant.
Example 7 screening of basal feeds
1. Preparation of basal feed
Test group basal feed: mixing 28kg of corn flour, 8kg of alfalfa meal, 10kg of bran, 14kg of soybean meal, 10kg of corn bran, 13kg of soybean hull, 12kg of wheat middling, 1.5kg of rumen bypass fat powder and 3.5kg of premix uniformly, and granulating;
control group basal feed: preparing a control group base feed according to a control group formula in the table 1;
the nutritional levels of the test group basal diet and the control group basal diet are shown in table 1.
2. Feeding of milk goats
The milk goat in the Dairy goat breeding community of Jingyang area of Shaanxi province is taken as a test object for feeding. 100 healthy dairy goats weighing 67kg on average at 2-3 fetuses and at the prophase of lactation were randomly divided into 2 groups (i.e., test group and control group), each of which contains 50 goats. The basic daily ration formula of each group is shown in table 1, the daily feeding is carried out for 45 days, the total milk yield of the milk goats of each group is recorded every day after the test is started, and finally, the average milk yield of each milk goat in each group in the test period is calculated. And collecting mixed milk samples of each group every day for detecting milk components including the milk fat rate, the milk protein rate and the content of trans fatty acid in total fatty acid in the last 4 days of the test, and finally calculating the average milk fat rate, the average milk protein rate and the content of trans fatty acid in total fatty acid in the average goat milk of each group. After feeding for 45 days, the average milk yield of the control group and the average milk yield of the test group are respectively 2.67 kg/day and 3.35 kg/day, and the average wool yield of each wool of the test group is 3.74 yuan/day more than that of the control group (the average sheep milk price is 5.5 yuan/kg); the average milk fat rate of the control group and the average milk fat rate of the test group are respectively 2.78 percent and 3.38 percent, and the average milk protein rate is respectively 3.28 percent and 3.30 percent; the average trans fatty acid content in the goat milk of the control group and the average trans fatty acid content in the total fatty acid content of the goat milk of the test group are respectively 5.40% and 3.27%, which do not meet the standard of producing infant milk powder, but from the overall data, the milk yield, the milk fat rate and the milk protein rate of the test group are all higher than those of the control group, and the trans fatty acid content in the total fatty acid content is obviously lower than that of the control group.
TABLE 1 daily ration formulas and nutritional levels
Example 8 screening and ratio determination of raw materials in functional composition for reducing trans-fatty acid content in ruminant milk
To further reduce the total fatty acid content of trans fatty acids in the sheep milk, tests were designed for different levels of addition of three functional additives (i.e., yeast culture, rumen bypass choline and rumen bypass methionine), i.e., a 3 x 3 orthogonal test, based on the basal diet formula of the experimental group of example 7.
Feeding the test subjects to 225 healthy milk goats with the average body weight of 68kg and the lactation number of 2-3 in the lactation prophase in a milk goat farm in Longxia county of Shanxi province according to L9(34) And (3) arranging an experiment in an orthogonal table, randomly dividing the experiment into 9 groups, feeding the goat for 45 days, recording the total milk yield of the dairy goats of each group every day after the experiment begins, and finally calculating the average milk yield of each goat every day in the experiment period. And collecting mixed milk samples of each group every day for detecting milk components including the milk fat rate, the milk protein rate and the content of trans fatty acid in total fatty acid in the last 4 days of the test, and finally calculating the average milk fat rate, the average milk protein rate and the content of trans fatty acid in total fatty acid in the average goat milk of each group. The results are shown in Table 3, from which it can be seen that: after the yeast culture, the rumen bypass choline and the rumen bypass methionine are added, compared with the test group in example 7, the average milk yield, the average milk fat rate and the average milk protein rate are all improved, and the proportion of trans fatty acid in the total fatty acid is reduced and is less than 3 percentThe formula is regulated in national standard infant formula (GB10765-2010) of food safety.
Data were analyzed using the general linear model in the SPSS analysis software and the results showed that there was a significant effect of the yeast culture on milk production (P < 0.05), milk fat ratio (P < 0.01) and the proportion of trans fatty acids to total fatty acids (P < 0.05). Rumen bypass choline (P < 0.05) and rumen bypass methionine (P < 0.01) had a significant effect on the ratio of trans fatty acids to total fatty acids. The group 1, 2 and 7 showed the best results in terms of combined milk production and trans fatty acid to total fatty acid ratio. The economic benefit index analysis (table 4) is combined, the group 2 has the highest profit, namely 2.5 g/day/yeast culture, 1.5 g/day rumen bypass choline and 3 g/day rumen bypass methionine, the effect is the best, and the group 1 and the group 7 have the next best effect.
TABLE 2 test protocol (units are g/only/day)
TABLE 3 test results
TABLE 4 economic benefit analysis
Example 9 determination of the proportion of a functional composition in a feed for reducing the trans-fatty acid content of ruminant milk
On the basis of the basic ration formula of the test group in example 7, the addition levels of three functional compositions (as shown in table 5) were designed, and the milk goats at the milk goat farm in shanxi longyuan county were used as test subjects for feeding. 150 healthy dairy goats, having an average body weight of 65kg, were randomly divided into 5 groups (i.e., A, B, C, D, E groups) of 30 in each group at the mid-lactation stage of 2-3 lactating births. Group A was fed the basic ration of the test group of example 7, group B, C, D, E was added with 10g, 20g, 30g and 40g of functional composition (wherein, the ratio of each component is 12.5% yeast culture, 7.5% rumen bypass choline, 15% rumen bypass methionine and 65% corncob meal) on the basis of the basic ration of group A, and fed for 45 days, after the test was started, the total milk yield of each group of dairy goats was recorded daily, and finally the average milk yield of each goat per day during the test period was calculated. And collecting mixed milk samples of each group every day for detecting milk components including the milk fat rate, the milk protein rate and the content of trans fatty acid in total fatty acid in the last 4 days of the test, and finally calculating the average milk fat rate, the average milk protein rate and the content of trans fatty acid in total fatty acid in the average goat milk of each group. The results show that: A. b, C, D and E groups had average milk yields of 2.45, 2.56, 2.82, 2.85 and 2.86 kg/day, respectively, milk fat ratios of 3.40%, 3.42%, 3.51%, 3.55% and 3.57%, milk protein ratios of 3.31%, 3.33%, 3.32%, 3.33%, and trans fatty acid ratios of 3.21%, 3.00%, 2.71%, 2.60% and 2.58%, respectively. Therefore, each sheep can achieve the aim of reducing the proportion of trans fatty acid in total fatty acid to be less than 3% by adding 20g-30 g/sheep per day (the addition amount is about 1% -1.5% of the basic feed), and the economic benefit is the best.
TABLE 5 daily ration formula and usage amount (unit is kg/day/piece)
Example 10 use of the feed of the invention for reducing the trans fatty acid content of ruminant milk
In order to further verify the feed use effect, the milk goats at a certain milk goat farm in Jingyang county, Shanxi were used as test subjects for feeding. 200 healthy milch goats with the average weight of 65kg at the middle lactation period of 2-3 lactation are randomly divided into 2 groups, and 100 healthy milch goats in each group are fed with the feed for reducing the trans-fatty acid content in the milk of the ruminants, which is described in the embodiment 4 and the embodiment 5 (the specific daily ration formula and the nutrition level are shown in the table 6 and the table 7). 100 healthy milch goats weighing 65kg on average in the middle and later stages of lactation after lactation of 2-3 lactation are fed with the feed for reducing the trans-fatty acid content in the milk of the ruminants, which is described in the example 6 (the specific daily ration formula and the nutrition level are shown in the table 8), after the test is started, the total milk yield of all groups of dairy goats is recorded every day, and finally, the average milk yield of each goat in each day in the test period is calculated. And collecting mixed milk samples of each group every day for detecting milk components including the milk fat rate, the milk protein rate and the content of trans fatty acid in total fatty acid in the last 4 days of the test, and finally calculating the average milk fat rate, the average milk protein rate and the content of trans fatty acid in total fatty acid in the average goat milk of each group.
The results show that after 40 days of feeding, the results show that: the average milk production of the flock fed the feed of example 4 was 2.70 kg/day. When the feeding is finished, the average milk fat rate of the flocks is 3.71%, the average milk protein rate is 3.3%, and the average proportion of trans fatty acid in the total fatty acid is 2.66%, which is in line with the production of infant milk powder.
After 40 days of feeding, the average milk production of the flock fed with the feed of example 5 was 2.68 kg/day. When the feeding is finished, the average milk fat rate of the flocks is 3.69%, the average milk protein rate is 3.25%, and the average proportion of trans fatty acid in the total fatty acid is 2.65%, which is in line with the production of infant milk powder.
After 40 days of feeding, the average milk production of the flock fed with the feed of example 6 was 2.50 kg/day. When the feeding is finished, the average milk fat rate of the flocks is 3.77 percent, the average milk protein rate is 3.31 percent, and the average proportion of trans fatty acid in the total fatty acid is 2.80 percent, which is in line with the production of infant milk powder.
TABLE 6 daily ration formula and nutritional level
TABLE 7 daily ration formulas and nutritional levels
TABLE 8 daily ration formulas and nutritional levels
It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations or modifications may be made on the basis of the above description, and all embodiments may not be exhaustive, and all obvious variations or modifications may be included within the scope of the present invention.
Claims (10)
1. A functional composition for reducing the content of trans fatty acid in ruminant milk is characterized by comprising the following components in percentage by weight: 10-15% of yeast culture, 5-10% of rumen bypass choline, 13-17% of rumen bypass methionine and 60-70% of carrier.
2. The functional composition of claim 1, wherein the carrier is a vegetable feed carrier.
3. Use of a functional composition according to claim 1 or 2 for the preparation of a feed for reducing the trans fatty acid content of ruminant milk.
4. A feed for reducing the content of trans fatty acids in ruminant milk, comprising: the feed comprises 0.8-1.5 wt% of the functional composition of claim 1 or 2.
5. The feed of claim 4, wherein: the feed also comprises the following components in percentage by weight: 10-35% of corn flour, 5-20% of soybean meal, 0-15% of cottonseed meal, 0-15% of alfalfa meal, 5-18% of corn bran, 10-18% of bran, 8-23% of soybean hull, 0-6% of corn protein powder, 5-15% of wheat middling, 0-5% of apple pomace, 0.5-2% of rumen bypass fat powder and 3-5% of premix.
6. The feed according to claim 4 or 5, characterized in that: the NDF content in the feed is 30% -50%, and the starch content is 15% -25%.
7. The functional composition of claim 1 or 2, or the use of the feed of any one of claims 4-6 in any one of:
u1, for use in reducing the proportion of trans fatty acids to total fatty acids in ruminant milk, or for use in preparing a product for reducing the proportion of trans fatty acids to total fatty acids in ruminant milk;
u2, use in improving the milk fat rate in ruminant milk, or, use in the preparation of a product for improving the milk fat rate in ruminant milk;
u3, in improving the milk yield of ruminants, or, in preparing a product for improving the milk yield of ruminants;
u4, use for improving the rumen health of ruminants, or use for the preparation of a product for improving the rumen health of ruminants.
8. A method of feeding a ruminant animal, the method comprising: feeding hay and the feed of claim 4 or 5 to a ruminant animal as a ration; wherein the hay is consumed freely and the feed is 1-3kg per feed per day.
9. The method of claim 8, wherein: the hay and the feed are fed in separate grooves; preferably, the feed is fed three times per day.
10. The functional composition according to claim 1 or 2, or the feed according to any one of claims 4 to 6, or the use according to claim 3 or 7, or the method according to claim 8 or 9, wherein: the ruminant is sheep; preferably a milk goat.
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