CN110699301A - Bacterial strain and application thereof in preventing and treating root rot of panax notoginseng - Google Patents
Bacterial strain and application thereof in preventing and treating root rot of panax notoginseng Download PDFInfo
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- CN110699301A CN110699301A CN201911120843.4A CN201911120843A CN110699301A CN 110699301 A CN110699301 A CN 110699301A CN 201911120843 A CN201911120843 A CN 201911120843A CN 110699301 A CN110699301 A CN 110699301A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a strain and application thereof in preventing and treating panax notoginseng root rot, wherein the strain has any one of nucleotide sequences shown as follows: i, has a nucleotide sequence shown in SEQ ID NO. 1; II, nucleotide sequence obtained by modifying, substituting, deleting or adding one or more bases in the nucleotide sequence shown in SEQ ID NO. 1. The strain has inhibitory effect on Notoginseng radix Fusarium sporotrichioides, Fusarium solani and phytophthora infestans.
Description
Technical Field
The invention relates to a strain and application thereof in preventing and treating panax notoginseng root rot, belonging to the field of biological pesticides.
Background
In recent years, with the rapid increase of the demand of traditional Chinese medicine resources, the traditional Chinese medicine wild resources are seriously damaged and are difficult to meet the market demand, and the change of wild traditional Chinese medicines into cultivation becomes a necessary way for meeting the market demand. Wild traditional Chinese medicinal materials are rarely damaged, and cannot form disasters even if the wild traditional Chinese medicinal materials are cultivated artificially, so that the growth environment of the traditional Chinese medicinal materials is changed. In an agricultural ecological system constructed manually, the variety of organisms is single, the plant density is increased, high water and high fertilizer are provided, manual remote seed regulation is performed, and the like, and the management measures create conditions for disease prevalence, so that diseases are easy to spread and propagate in the population, between fields and between regions, and the diseases are easy to infect by artificially cultured medicinal materials. Such problems exist if large-area artificial planting of panax notoginseng is carried out.
Pseudo-ginseng (Panax notoginseng (Burk.) F.H.Chen) is a perennial herb of Panax of Araliaceae, and is a rare Chinese medicinal material in China. The pseudo-ginseng is perennial root plant, prefers warm and humid environment, and provides favorable conditions for diseases due to single planting of the pseudo-ginseng in large area in successive years.
The main diseases of pseudo-ginseng are root rot, and the main pathogenic bacteria are as follows: pillowcases (fungi, c.didymum), Fusarium solani (Fusarium solani), Fusarium oxysporum (Fusarium oxysporum), phoma (phoma herbare), rhizoctonia solani (rhizoctonia), phytophthora infestans (phytophthora parasiticum), Pseudomonas (Pseudomonas spp.), Meloidogyne (meloidogenin spp.), and the like. The root rot can reduce the yield of the panax notoginseng by 5-20%, and can reach more than 70% in serious cases, the loss caused by the root rot accounts for 70-85% of various diseases of the panax notoginseng, and the root rot becomes one of the important restriction factors of the panax notoginseng planting industry at present.
At present, the diseases and the pests of the traditional Chinese medicinal materials are mainly prevented and controlled by spraying chemical agents, long-term use of chemical pesticides can cause environmental pollution, damage to an ecosystem, easily generate pathogenic bacteria with drug resistance, and even bring harm to the traditional Chinese medicinal materials such as pesticide residue and heavy metal pollution, so that the use of the pesticides is reasonably reduced, a green and environment-friendly prevention and control mode is found, and the method has important significance for preventing and controlling the diseases and the pests of the traditional Chinese medicinal materials.
Disclosure of Invention
According to one aspect of the present invention, a strain is provided.
According to one aspect of the invention, the application of the strain in preventing and treating root rot of panax notoginseng is provided, and the strain is used for solving the problem that panax notoginseng is susceptible to root rot and affects the yield of panax notoginseng when the panax notoginseng is artificially planted in a large area for a long time; the chemical pesticide is adopted to prevent and treat panax notoginseng infectious germs, which is easy to pollute the environment, destroy the ecosystem and generate pathogenic bacteria with drug resistance.
In one aspect of the present invention, there is also provided a strain having any one of the nucleotide sequences shown below:
i, has a nucleotide sequence shown in SEQ ID NO. 1;
II, nucleotide sequence obtained by modifying, substituting, deleting or adding one or more bases in the nucleotide sequence shown in SEQ ID NO. 1.
In some embodiments of the invention, the strain provided by the invention is Serratia plymuthica (Serratia plymuthica).
In some embodiments of the invention, the strain provided by the invention has a collection number of CGMCCNo.18387.
In some embodiments of the invention, the strain provided by the invention is a gram-negative bacterium, and the bacterium is rod-shaped; bacterial colonies of the strain are light yellow on an NA flat plate, the surface of the bacterial colonies is smooth and raised, and the edges of the bacterial colonies are neat and round.
The invention also provides an application of the strain in preventing and treating panax notoginseng root rot.
Optionally, the inhibiting comprises: inhibiting the hypha growth and spore germination of Fusarium oxysporum, Fusarium solani and Phytophthora infestans.
Optionally, the panax notoginseng root rot is a panax notoginseng root rot caused by panax notoginseng fusarium oxysporum, fusarium solani and panax notoginseng phytophthora infestans.
The invention also provides a method for preventing and treating panax notoginseng root rot by using the bacterial strain, which at least comprises the following steps:
activating the strain to prepare fermentation liquor;
and (3) pouring the liquid containing the bacterial liquid into the root of the pseudo-ginseng plant.
In a specific embodiment, the strain PBLJ042 is inoculated in an NA culture medium plate, inverted culture is carried out for 2 days at 28 ℃, the strain is selected and inoculated in 1000ml of NA liquid culture solution, and the solution is cultured for 2 days at the temperature of 28 ℃ and the rotating speed of 200r/min to obtain fermentation liquor.
Optionally, the notoginseng plants are plants at the seedling stage and the adult stage.
Optionally, after the fermentation liquid is diluted by 20 times, the fermentation liquid is injected into the roots of the plants according to 20ml per plant, and the fermentation liquid is continuously used for 2-3 times at intervals of 30 days each time.
In one embodiment, the method for screening strains comprises the following steps:
collecting healthy rhizoma paridis tuber from Lijiang city of Yunnan province, soaking the tuber in 75% ethanol for 1min, washing with sterile water for 3 times, and drying with sterile filter paper; HgCl with the mass concentration of 0.2 percent2Soaking the tuber for 3min, washing with sterile water for 10 times, and drying surface water with sterile filter paper to sterilize the tuber.
The surface-sterilized Paris polyphylla tuber skin was cut with a sterile scalpel, cut into 0.5X 0.5cm cubes, placed in NA medium plates, and cultured at 28 ℃ for 2 days. After the bacteria grow out on the plate, collecting the strains, purifying for 3-5 times at 28 ℃ by adopting a plate streaking purification method, wherein the obtained purified strain is numbered as a strain PBLJ042, and respectively inoculating pseudo-ginseng pathogenic bacteria on each PDA culture medium: fusarium sporotrichioides, fusarium solani and phytophthora infestans, and strains PBLJ042 are inoculated near pseudo-ginseng pathogenic bacteria inoculation points of various PDA culture media in a streak manner. Then cultured at 28 ℃ for 3 days. And testing the antagonism of the bacterial strain PBLJ042 to each pathogenic bacterium of the pseudo-ginseng by adopting a antagonism method.
The invention can produce the beneficial effects that:
1) the strain provided by the invention has an inhibiting effect on pseudo-ginseng fusarium sporotrichioides, fusarium solani and phytophthora infestans at the same time.
2) The bacterial strain provided by the invention can effectively prevent and treat the panax notoginseng root rot caused by panax notoginseng fusarium oxysporum, fusarium solani and panax notoginseng phytophthora infestans, can effectively reduce the use of chemical pesticides, realizes environmental friendliness and avoids the generation of drug-resistant pathogenic bacteria.
3) The strain provided by the invention can be applied to the prevention and treatment of pseudo-ginseng root rot.
Biological preservation description:
biological material: the strain PBLJ042 is classified and named as Serratia plymuthica (Serratia plymuthica), and is preserved in the China general microbiological culture Collection center (CGMCC) in 2019, 8 and 15 days, and the preservation unit address is as follows: no. 3 of Xilu No.1 of Beijing, Chaoyang, the preservation number is CGMCC No. 18387.
Drawings
FIG. 1 is a colony morphology schematic diagram of the strain PBLJ042 provided by the invention;
FIG. 2 is a schematic diagram of a 16S NJ phylogenetic tree of the strain PBLJ042 provided by the invention;
FIG. 3 is a schematic diagram of the results of experiments of plate confrontation of the strain PBLJ042 provided by the invention with phytophthora infestans, fusarium solani and fusarium oxysporum respectively; wherein, a) is a colony photo of a phytophthora infestans control group; b) a colony photo of a phytophthora infestans treatment group; c) a colony picture of a fusarium solani treatment group; d) a colony picture of a fusarium solani control group is shown; e) a photograph of a colony of a fusarium oxysporum treated group; f) a colony picture of a fusarium oxysporum control group is obtained;
FIG. 4 is a scanning electron microscope structural photograph of the strain PBLJ 042.
Detailed Description
The present invention will be described in detail with reference to examples, but the present invention is not limited to these examples.
Examples
Unless otherwise specified, the raw materials, germs, and reagents in the examples of the present invention were all purchased commercially.
Example 1 isolation and screening of strains the present invention provides
Collecting healthy rhizoma paridis tuber from Yunnan Lijiang, soaking the tuber in 75% ethanol for 1min, washing with sterile water for 3 times, and drying with sterile filter paper; HgCl with the mass concentration of 0.2 percent2Soaking the tuber for 3min, washing with sterile water for 10 times, and drying surface water with sterile filter paper to sterilize the tuber.
The surface-sterilized Paris polyphylla tuber skin was cut with a sterile scalpel, cut into 0.5X 0.5cm cubes, placed in NA medium plates, and cultured at 28 ℃ for 2 days. After bacteria grow out on the flat plate, collecting the bacterial strain, and purifying for 3-5 times at 28 ℃ by adopting a flat plate streak separation and purification method to obtain the purified bacterial strain with the serial number of bacterial strain PBLJ 042.
Respectively inoculating pseudo-ginseng pathogenic bacteria on each PDA culture medium: fusarium sporotrichioides, fusarium solani and phytophthora infestans, and strains PBLJ042 are inoculated near pseudo-ginseng pathogenic bacteria inoculation points of various PDA culture media in a streak manner. Then cultured at 28 ℃ for 3 days.
The antagonism of the bacterial strain PBLJ042 to pathogenic bacteria of panax notoginseng is tested by adopting a antagonism method, and the result shows that the bacterial strain has antagonism to fusarium solani, fusarium solani and phytophthora infestans (shown in figure 3).
Example 2 Strain identification
1. Morphological feature observation
And (3) colony morphology characteristics: after the strain PBLJ042 was inoculated on the NA plate, it was cultured at 28 ℃ for 2 days to obtain a colony. The bacterial colony formed by the bacterial strain PBLJ042 is shown in figure 1, and the bacterial colony is light yellow on an NA plate, has a smooth surface, is raised, and has a neat and round edge.
Morphological characteristics of the thallus: according to the existing gram bacteria identification method, after the bacterial strain PBLJ042 is dyed, red is displayed, and the bacterial strain is gram-negative bacteria. Scanning electron microscope shows strain PBLJ042, and the obtained result is shown in figure 4, the thallus of the strain PBLJ042 is rod-shaped, and the size is (0.32-0.64) Mumx (0.84-1.30) Mum.
2.16S sequence analysis
After extracting the genomic DNA of the strain by adopting a liquid nitrogen grinding method, adopting a primer: 27F 5 '-agatttgatttcctggctcag 3'; 1492R 5'-TACGGCTACCTTGTTACGACTT-3' was subjected to PCR amplification.
After gel electrophoresis, the gel is sent to bioengineering (Shanghai) Limited company for sequencing, and the total length of the sequence is 1387bp (shown as SEQ). The obtained sequence is submitted to a GenBank database for BLAST analysis and comparison, and a strain with higher homology (the homology can reach 99.86%) with PBLJ042 belongs to Serratia sp.
An NJ phylogenetic tree was constructed with the 16S sequence, as shown in FIG. 2, and the strain PBLJ042 was in the same branch as Serratia plymuthica.
According to the morphological feature analysis, ITS analysis and NJ phylogenetic tree, the strain PBLJ042 is preliminarily identified to be serratia plymuthica.
Example 3 determination of bacteriostatic Activity of Strain PFLJ04 on pathogenic bacteria of Panax notoginseng
Culturing the strain PBLJ 0422 days on an NA culture medium at 28 ℃, taking common pathogenic bacteria of fusarium oxysporum, fusarium solani and phytophthora infestans in panax notoginseng root rot as pathogen target bacteria, testing the antibacterial activity of the strain PBLJ042 by adopting a confrontation culture method, taking a blank group as a control group, inoculating the strain PBLJ042 by streaking the pathogen target bacteria, and culturing under the same conditions (culturing for 2 days on the NA culture medium at 28 ℃).
Colony pictures in the blank group are shown as a) and d) in figure 3, colony pictures in the blank group of the panax notoginseng phytophthora infestans are shown as a) in figure 3, the colony edges are complete and symmetrical, hyphae cover the whole culture medium, the phytophthora infestans treatment group is shown as b) in figure 3, after the strain PFLJ04 is subjected to streak culture beside the pathogenic target bacteria, the phytophthora infestans is inhibited, the pathogenic bacteria colonies are asymmetrical, the color is relatively pale in the blank group, and a bright bacterial inhibition zone is formed between the pathogenic bacteria colonies and the bacterial strain PFLJ 04.
The colony picture of the blank group of the fusarium solani is shown in d) in fig. 3, the edge of the colony in the blank group is complete, and the whole color of the colony covering the central area of the culture medium permeates into the culture medium. The treatment group is as shown in c) in fig. 3, in the treatment group, after the strain PFLJ04 is subjected to streak culture beside the pathogenic target bacteria, the growth of fusarium solani is inhibited, the formed colonies are asymmetric, a remarkable inhibition zone is formed at one side close to the colonies of the strain PFLJ04, and the color of the colonies of pathogenic bacteria is relatively light compared with that of the blank group.
F) in figure 3, the colony edge of the blank group of the panax notoginseng fusarium oxysporum is complete, the central area of the culture medium is covered, and the colony is integrally symmetrical. The treatment group is shown as e) in fig. 3, in the treatment group, after the strain PFLJ04 is streaked and cultured beside the pathogenic target bacteria, the growth of the fusarium notoginseng is inhibited, the formed colony is asymmetric, and a remarkable inhibition zone is formed at one side close to the colony of the strain PFLJ 04.
As can be seen from FIG. 3, in the confrontation experiment, the strain PFLJ04 has obvious effects of inhibiting growth, development and proliferation of fusarium oxysporum, fusarium solani and phytophthora infestans.
The colony sizes in a) to f) of FIG. 3 were measured and the inhibition rates were calculated according to the following formula:
inhibition (%) - (control target colony radius-treatment target colony radius)/control target colony radius x 100%
The control group target colony radius is the growth radius of the panax notoginseng root rot germs, and the treatment group target colony radius is the growth radius of the panax notoginseng root rot germs between the central point of the panax notoginseng root rot germs and the center of the serratia line. The results are shown in Table 1.
TABLE 1 bacteriostatic activity of the strain PBLJ042 against pathogenic bacteria of Panax notoginseng
As can be seen from the table 1, the bacterial strain PBLJ042 has the smallest diameter of the inhibition zone for the phytophthora infestans, and the inhibition rate can reach 79.1 percent; the diameter of a bacteriostatic circle for fusarium solani is the largest, and the bacteriostatic rate can reach 88.7%; the lowest inhibition rate of pathogenic bacteria in 3 in table 1 can also reach 77.3%.
EXAMPLE 4 preparation of Strain PBLJ042 fermentation broth
Inoculating the strain PBLJ042 into an NA culture medium plate, performing inverted culture at 28 ℃ for 2 days, activating the strain, selecting the strain, inoculating the strain into 1000ml of NA liquid culture solution, and culturing for 2 days at the temperature of 28 ℃ and the rotating speed of 200r/min to obtain the strain PBLJ042 fermentation liquor.
Example 5 identification of prevention and treatment effects of PBLJ042 strain fermentation broth on Fusarium solani f.f. sp.notoginseng
1. Preparing a panax notoginseng fusarium solani solution with pathogenic bacteria of panax notoginseng root rot:
after Fusarium solani is activated on the PDA culture medium, a blade is used for scraping off mycelium, and the mycelium is added into the liquid PDA culture medium for culture (under the condition of 200r/min and 28 ℃) for 7 days to obtain the pseudo-ginseng Fusarium solani bacterial liquid.
2. The strain PBLJ042 fermentation liquor is diluted by 20 times for standby.
3. Root irrigation experiment:
experiment raw materials: 1 year-old pseudo-ginseng seedlings;
experiment is as follows: CK group, using inoculated sterile distilled water as control, inoculating 10ml sterile distilled water per plant at root of notoginseng seedling, applying for 1 time;
in the SI group, 20 ml/plant of fusarium solani f.solani is inoculated at the root of the pseudo-ginseng seedling and applied for 1 time;
DI group: inoculating mixed liquor 40 ml/plant of Fusarium solani bacterial liquid of Notoginseng and bacterial strain PBLJ042 fermentation liquor at root of Notoginseng seedling, and applying for 1 time. The fusarium solani bacterial liquid of panax notoginseng and the bacterial strain PBLJ042 fermentation liquid are mixed according to the volume ratio of 1: 1.
Each treatment group was repeated 3 times, and each repetition was inoculated with 20 seedlings of notoginseng. After 30 days, the morbidity, disease index and prevention rate are counted.
4. Calculating disease index and prevention effect
The growth of pseudo-ginseng seedlings in each treatment was observed, and the disease grade was graded and scored according to the following table.
The disease grade standard is as follows:
level 0: no disease at root;
level 1: the scab accounts for less than 20% of the surface area of the root;
and 2, stage: the scab accounts for 21-40% of the surface area of the root;
and 3, level: the spots account for 41-65% of the surface area of the root, and the appearance is seriously affected;
4, level: lesions account for more than 66% of the surface area of the root or are completely rotten.
According to the statistical result, the morbidity of each control group and treatment group is respectively calculated according to the following formula:
incidence (%) — number of diseased plants/total number of experimental plants in each group × 100%;
according to the statistical result, the disease indexes of the control group and the treatment group are respectively calculated according to the following formula:
disease index ═ Σ (number of diseased plants at each stage × number of stages of the disease)/(number of investigated total plants × number of stages of highest disease stage) × 100%
According to the statistical result, the control effect is calculated according to the following formula:
the control rate (%) is (control disease index-treatment group disease index)/control disease index x 100%, and the treatment groups herein include SI group and DI group.
The control, treatment group control rate, disease index results are listed in table 2.
TABLE 2 prevention and control effect of PBLJ042 strain on Fusarium solani
| Treatment group | Incidence of disease | Index of disease condition | Rate of prevention and cure |
| CK group | 0％ | - | - |
| SI group | 95.6％ | 47.8 | - |
| DI group | 56.2％ | 24.6 | 48.5％ |
As shown in Table 2, the control group had a disease incidence of 0 because it was not infected with germs. The disease incidence in the SI group is 95.6 percent, which shows that the root-irrigation germ liquid has extremely high disease-infecting performance to plants. The incidence of the DI group is reduced by 50% compared with the SI group, which shows that the bacterial strain PBLJ042 has better inhibiting effect on the growth of the fusarium solani, can prevent the growth of pathogenic bacteria in the plant, reduces the infection probability and achieves the effect of preventing diseases.
The disease index of the DI group is obviously lower than that of the SI group, which shows that the disease grade of the diseased plants in the DI group is lower, and the bacterial strain PBLJ042 has better inhibition effect on the growth of the fusarium solani f.f. pseudo-ginseng, and can prevent the plants infected with pathogenic bacteria from aggravating the disease.
The prevention and treatment rate of the DI group is close to 50%, which shows that the bacterial strain has better treatment effect on the fusarium solani. As can be seen from Table 2, the strain has a good disease control effect in the seedling stage of pseudo-ginseng.
Example 6 prevention and treatment experiment of Panax notoginseng in adult stage
Taking 60 panax notoginseng plants infected with fusarium solani in the adult stage, dividing into 3 groups, and taking 20 panax notoginseng plants in each group as one repeat. The fermentation broth obtained in example 4 was applied as follows for each repetition.
After the fermentation liquor is diluted by 20 times, root irrigation treatment is carried out on each repeated plant according to 20 ml/plant, the treatment interval is 30 days, the root irrigation of each plant is carried out for 2 times in each treatment, and the disease infection and cure conditions of the plants are counted after 60 days.
The effect obtained in this example is similar to that of example 5, which shows that the strain has the same therapeutic effect on panax notoginseng in adult stage.
Reference throughout this specification to "one embodiment," "another embodiment," "an embodiment," "a preferred embodiment," or the like, means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present general inventive concept. The appearances of the same phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is within the scope of the invention to effect such feature, structure, or characteristic in connection with other embodiments.
Although the invention has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, the drawings and the appended claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
SEQUENCE LISTING
<110> university of Yunnan
<120> bacterial strain and application thereof in preventing and treating panax notoginseng root rot
<130>1
<160>1
<170>PatentInversion3.3
<210>1
<211>1387
<212>DNA
<213> Serratia plymuthica (Serratia plymuthica)
<400>1
tgcagtcgag cggaagcaca ggagagcttg ctctctgggt gacgagcggc ggacgggtga 60
gtaatgtctg ggaaactgcc tgatggaggg ggataactac tggaaacggt agctaatacc 120
gcataacgtc tacggaccaa agtgggggac cttcgggcct cacgccatca gatgtgccca 180
gatgggatta gctagtaggt ggggtaatgg ctcacctagg cgacgatccc tagctggtct 240
gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg ggaggcagca 300
gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg tgtgaagaag 360
gccttagggt tgtaaagcac tttcagcgag gaggaagggt tcagtgttaa tagcactgtg 420
cattgacgtt actcgcagaa gaagcaccgg ctaactccgt gccagcagcc gcggtaatac 480
ggagggtgca agcgttaatc ggaattactg ggcgtaaagc gcacgcaggc ggtttgttaa 540
gtcagatgtg aaatccccgc gcttaacgtg ggaactgcat ttgaaactgg caagctagag 600
tcttgtagag gggggtagaa ttccaggtgt agcggtgaaa tgcgtagaga tctggaggaa 660
taccggtggc gaaggcggcc ccctggacaa agactgacgc tcaggtgcga aagcgtgggg 720
agcaaacagg attagatacc ctggtagtcc acgctgtaaa cgatgtcgat ttggaggttg 780
tgcccttgag gcgtggcttc cggagctaac gcgttaaatc gaccgcctgg ggagtacggc 840
cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga gcatgtggtt 900
taattcgatg caacgcgaag aaccttacct actcttgaca tccagagaac tttccagaga 960
tggattggtg ccttcgggaa ctctgagaca ggtgctgcat ggctgtcgtc agctcgtgtt 1020
gtgaaatgtt gggttaagtc ccgcaacgag cgcaaccctt atcctttgtt gccagcgatt 1080
cggtcgggaa ctcaaaggag actgccggtg ataaaccgga ggaaggtggg gatgacgtca 1140
agtcatcatg gcccttacga gtagggctac acacgtgcta caatggcgta tacaaagaga 1200
agcgaactcg cgagagcaag cggacctcat aaagtacgtc gtagtccgga ttggagtctg 1260
caactcgact ccatgaagtc ggaatcgcta gtaatcgtag atcagaatgc tacggtgaat 1320
acgttcccgg gccttgtaca caccgcccgt cacaccatgg gagtgggtgc aaaagaagta 1380
ggtagct 1387
Claims (10)
1. A strain having any one of the nucleotide sequences shown below:
i, has a nucleotide sequence shown in SEQ ID NO. 1;
II, nucleotide sequence obtained by modifying, substituting, deleting or adding one or more bases in the nucleotide sequence shown in SEQ ID NO. 1.
2. The strain of claim 1, which is Serratia plymuthica.
3. The strain of claim 1, having a accession number of CGMCC No. 18387.
4. The strain according to claim 1, wherein the strain is gram-negative bacteria, and the bacteria are rod-shaped; bacterial colonies of the strain are light yellow on an NA flat plate, the surface of the bacterial colonies is smooth and raised, and the edges of the bacterial colonies are neat and round.
5. The application of the bacterial strain according to any one of claims 1-4 in controlling panax notoginseng root rot.
6. The use of claim 5, wherein said suppressing comprises: inhibiting the hypha growth and spore germination of Fusarium oxysporum, Fusarium solani and Phytophthora infestans.
7. The use of claim 5, wherein the Panax notoginseng root rot is caused by Fusarium oxysporum, Fusarium solani, and Phytophthora infestans.
8. A method for controlling panax notoginseng root rot by using the strain as described in any one of claims 1 to 4, comprising at least the following steps:
activating the strain to prepare fermentation liquor;
and (3) pouring the liquid containing the bacterial liquid into the root of the pseudo-ginseng plant.
9. The method of claim 8, wherein the panax notoginseng plant is a seedling stage and adult stage plant.
10. The method according to claim 8, wherein the fermentation liquid is diluted by 20 times, and the fermentation liquid is injected into the roots of the plants according to 20ml per plant, and is continuously used for 2-3 times at intervals of 30 days.
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Cited By (4)
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|---|---|---|---|---|
| CN110982708A (en) * | 2019-12-23 | 2020-04-10 | 云南大学 | Bacterial strain and application thereof in bioremediation of heavy metal polluted environment |
| CN114806892A (en) * | 2022-04-28 | 2022-07-29 | 云南省农业科学院生物技术与种质资源研究所 | Trichoderma atroviride strain and application thereof in preventing and treating panax notoginseng root rot |
| CN114907986A (en) * | 2022-04-28 | 2022-08-16 | 云南省农业科学院生物技术与种质资源研究所 | Trichoderma harzianum and application thereof in preparation for preventing and treating root rot of panax notoginseng |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982708A (en) * | 2019-12-23 | 2020-04-10 | 云南大学 | Bacterial strain and application thereof in bioremediation of heavy metal polluted environment |
| KR20220129341A (en) * | 2021-03-16 | 2022-09-23 | 전남대학교산학협력단 | Composition for controlling plant bacterium disease comprising culture solution of Serratia plymuthica C-1 Strain or extract thereof, manufacturing methods thereof and controlling method for plant bacterium disease |
| KR102564694B1 (en) | 2021-03-16 | 2023-08-08 | 전남대학교산학협력단 | Composition for controlling plant bacterium disease comprising culture solution of Serratia plymuthica C-1 Strain or extract thereof, manufacturing methods thereof and controlling method for plant bacterium disease |
| CN114806892A (en) * | 2022-04-28 | 2022-07-29 | 云南省农业科学院生物技术与种质资源研究所 | Trichoderma atroviride strain and application thereof in preventing and treating panax notoginseng root rot |
| CN114907986A (en) * | 2022-04-28 | 2022-08-16 | 云南省农业科学院生物技术与种质资源研究所 | Trichoderma harzianum and application thereof in preparation for preventing and treating root rot of panax notoginseng |
| CN114907986B (en) * | 2022-04-28 | 2022-11-18 | 云南省农业科学院生物技术与种质资源研究所 | Trichoderma harzianum and application thereof in preparation for preventing and treating root rot of panax notoginseng |
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