CN110668862A - Nutrient solution formula for promoting wheat seedling growth and nitrogen absorption and preparation method thereof - Google Patents
Nutrient solution formula for promoting wheat seedling growth and nitrogen absorption and preparation method thereof Download PDFInfo
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Abstract
The invention is suitable for the technical field of wheat planting, and provides a nutrient solution formula for promoting the growth of wheat seedlings and nitrogen absorption and a preparation method thereof, wherein the formula comprises calcium nitrate tetrahydrate, anhydrous potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, disodium ferric ethylenediamine tetraacetate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, zinc sulfate heptahydrate and sodium silicate nonahydrate, the preparation method is simple, easy to store and ideal in product form, not only contains rich nutrient substances required by the growth and development of wheat, meets the growth conditions of the wheat seedlings, but also contains zinc, silicon and other nutrient elements, the nutrient solution is used for soaking and culturing the wheat, the germination rate of the wheat can reach more than 90 percent, the growth phenotype of the wheat is improved, and the absorption and utilization rate of the nitrogen of the wheat seedlings are obviously improved, so that the problem of low nitrogen utilization rate of the indoor wheat is solved, the reasonable and balanced utilization of nutrients is realized, and the resource waste is reduced.
Description
Technical Field
The invention belongs to the technical field of wheat planting, and particularly relates to a nutrient solution formula for promoting wheat seedling growth and nitrogen absorption and a preparation method thereof.
Background
Wheat is the second major food crop in China, and the varieties of the wheat can be generally divided into two types: spring wheat and winter wheat. Nitrogen is one of the essential macronutrients for plant growth and development. Winter wheat requires more nitrogen elements than spring wheat types because it requires a longer growing period and experiences a cold winter to vernalize. In the last 30 years, wheat nitrogen usage has increased in developing countries in order to increase yield. However, only 30% -35% of the added nitrogen is absorbed by wheat. This phenomenon causes environmental pollution such as greenhouse gases in the atmosphere, and the like. Therefore, the improvement of the absorption and utilization rate of wheat nitrogen is urgent.
The plant nutrient solution is widely applied to scientific research, agriculture and indoor plant planting, and compared with the soil environment, factors such as pH, nutrients, microorganisms and the like in the nutrient solution are very easy to control, so that the environment of the nutrient solution is relatively stable for indoor growing plants. The culture of wheat and the absorption capacity of wheat to nitrogen play a great role in agricultural production, and more people in cities regard wheat seedlings as indoor ornamental plants to increase the life interest. Compared with soil cultivation, nutrient solution cultivation is more suitable for people living in cities to cultivate wheat; meanwhile, the cultivation of indoor wheat seedlings is a key step capable of improving the survival rate and the yield of wheat transplantation in the later period, and is concerned with agriculture and civil life. Therefore, the method has very important significance for creating the nutrient solution capable of improving the nitrogen absorption of the wheat. At present, no product for improving nitrogen absorption of wheat seedlings through nutrient solution cultivation and relevant explicit reports exist.
Disclosure of Invention
The invention provides a nutrient solution formula for promoting wheat seedling growth and nitrogen absorption and a preparation method thereof, and aims to improve the nitrogen absorption of wheat seedlings.
The invention is realized in this way, a nutrient solution formula for promoting wheat seedling growth and nitrogen absorption and its preparation method, the formula includes:
calcium nitrate tetrahydrate, anhydrous potassium sulfate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium iron ethylenediaminetetraacetate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, zinc sulfate heptahydrate and sodium silicate nonahydrate.
The invention also provides a preferable formula, which comprises the following components in concentration ratio:
1.5-2.5mM of calcium nitrate tetrahydrate, 2.5-3mM of anhydrous potassium sulfate, 1-1.5mM of monopotassium phosphate, 2-2.5mM of magnesium sulfate heptahydrate, 100 mu M of disodium iron ethylenediaminetetraacetate, 46-55 mu M of boric acid, 9-12 mu M of manganese chloride tetrahydrate, 0.3-0.5 mu M of copper sulfate pentahydrate, 0.02-0.03 mu M of ammonium molybdate tetrahydrate, 5-20 mu M of zinc sulfate heptahydrate and 10-20 mu M of sodium silicate nonahydrate.
The invention also provides a preparation method of the nutrient solution for promoting wheat seedling growth and nitrogen absorption, which is suitable for any one of the above nutrient solution formulas for promoting wheat seedling growth and nitrogen absorption, and the preparation method comprises the following steps:
1): dissolving 4.45g of ferrous sulfate heptahydrate in 200ml of distilled water to obtain a ferrous sulfate heptahydrate solution, dissolving 5.96g of disodium ethylene diamine tetraacetate in 200ml of distilled water to obtain a disodium ethylene diamine tetraacetate solution, heating the disodium ethylene diamine tetraacetate solution, slowly adding the ferrous sulfate heptahydrate solution into the disodium ethylene diamine tetraacetate while stirring, then chelating in an oven, cooling to a constant volume of 1L, and storing in a first reagent bottle to obtain a mother solution A;
2): respectively preparing 1000 multiplied mother liquor B of calcium nitrate tetrahydrate, anhydrous potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate and zinc sulfate heptahydrate, fixing the volume to 1L, and storing in a second reagent bottle;
3): respectively absorbing 1mL of mother liquor A and 1mL of mother liquor B, dissolving in 999mL of deionized water, shaking up to obtain a solution C;
4): and (4) storing the solution C obtained in the step (3) in a third reagent bottle to obtain the nutrient solution.
The invention also provides that preferably, the pH value of the solution C is 5.5-5.8.
Preferably, in the step 1, the heating temperature of the disodium ethylene diamine tetraacetate solution is 70 ℃.
The invention also provides a preferable mode, and the first reagent bottle, the second reagent bottle and the third reagent bottle are brown reagent bottles.
The invention also provides that the temperature of the oven in the step 1 is 70 ℃ and the chelation time is 4 h.
The invention also provides that in the step 3, the absorbed mother liquor A and the absorbed mother liquor B are respectively 0.5 ml.
Compared with the prior art, the invention has the beneficial effects that: the invention has simple preparation method, easy preservation and ideal product form, not only contains rich nutrient substances required by the growth and development of wheat, satisfies the growth conditions of wheat seedlings, but also contains zinc, silicon and other nutrient elements, and the nutrient solution is used for soaking and culturing the wheat, so that the germination rate of the wheat can reach more than 90 percent, the growth phenotype of the wheat is improved, and simultaneously, the absorption and utilization rate of the wheat to nitrogen are obviously improved, thereby improving the problem of low nitrogen utilization rate of indoor wheat seedlings, realizing reasonable and balanced utilization of nutrients and reducing resource waste.
Drawings
Fig. 1 is a schematic flow chart of a method for preparing a nutrient solution according to a first embodiment of the present invention.
FIG. 2 is a graph showing the results of the first experiment according to the present invention.
FIG. 3 is a graph showing the results of the second experiment according to the present invention.
FIG. 4 is a graph showing the results of the third experiment in accordance with the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example one
The embodiment provides a technical scheme: a nutrient solution formula for promoting wheat seedling growth and nitrogen absorption comprises the following components in percentage by concentration:
1.5-2.5mM of calcium nitrate tetrahydrate, 2.5-3mM of anhydrous potassium sulfate, 1-1.5mM of monopotassium phosphate, 2-2.5mM of magnesium sulfate heptahydrate, 100 mu M of disodium iron ethylenediaminetetraacetate, 46-55 mu M of boric acid, 9-12 mu M of manganese chloride tetrahydrate, 0.3-0.5 mu M of copper sulfate pentahydrate, 0.02-0.03 mu M of ammonium molybdate tetrahydrate, 5-20 mu M of zinc sulfate heptahydrate and 10-20 mu M of sodium silicate nonahydrate.
The calcium nitrate tetrahydrate provides nitrogen for the growth of wheat, the anhydrous potassium sulfate provides potassium, the monopotassium phosphate provides phosphorus and potassium, the magnesium sulfate heptahydrate provides magnesium, the disodium ferric ethylenediaminetetraacetate provides iron, the boric acid provides trace element boron, the manganese chloride tetrahydrate provides trace element manganese, the copper sulfate pentahydrate provides trace element copper, the ammonium molybdate tetrahydrate provides trace element molybdenum, the zinc sulfate heptahydrate provides zinc, and the sodium silicate nonahydrate provides silicon.
The nutrient solution is then prepared according to the following steps, please refer to fig. 1:
1): dissolving 4.45g of ferrous sulfate heptahydrate in 200ml of distilled water to obtain a ferrous sulfate heptahydrate solution, dissolving 5.96g of disodium ethylene diamine tetraacetate in 200ml of distilled water to obtain a disodium ethylene diamine tetraacetate solution, heating the disodium ethylene diamine tetraacetate solution by adopting a 70 ℃ water bath, slowly adding the ferrous sulfate heptahydrate solution into the disodium ethylene diamine tetraacetate while stirring, then chelating for 4 hours in a 70 ℃ oven, cooling to a constant volume of 1L, and storing in a first brown reagent bottle to obtain a mother solution A.
2): respectively preparing 1000 multiplied mother liquor B of calcium nitrate tetrahydrate, anhydrous potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, zinc sulfate heptahydrate and sodium silicate nonahydrate, metering the volume to 1L, and storing in a second brown reagent bottle.
3): absorbing total 1mL of mother liquor A and mother liquor B respectively, dissolving the mother liquor A and the mother liquor B which are absorbed in 999mL of deionized water to respectively obtain 0.5mL of mother liquor A and mother liquor B, shaking up to obtain solution C.
4): and (4) adjusting the pH value of the solution C in the step (3) to 5.5-5.8, and storing in a third brown reagent bottle to obtain the nutrient solution.
The invention has simple preparation method, easy preservation and ideal product form, not only contains rich nutrient substances required by the growth and development of wheat, satisfies the growth conditions of wheat seedlings, but also contains zinc, silicon and other nutrient elements, and the nutrient solution is used for soaking and culturing the wheat, so that the germination rate of the wheat can reach more than 90 percent, the growth phenotype of the wheat is improved, and simultaneously, the absorption and utilization rate of the wheat to nitrogen are obviously improved, thereby improving the problem of low nitrogen utilization rate of indoor wheat seedlings, realizing reasonable and balanced utilization of nutrients and reducing resource waste.
Example two
The embodiment provides a technical scheme: a nutrient solution formula for promoting wheat seedling growth and nitrogen absorption and a preparation method thereof are disclosed, the formula comprises the following components according to the concentration ratio:
1.5-2.5mM of calcium nitrate tetrahydrate, 2.5-3mM of anhydrous potassium sulfate, 1-1.5mM of monopotassium phosphate, 2-2.5mM of magnesium sulfate heptahydrate, 100 mu M of disodium iron ethylenediaminetetraacetate, 46-55 mu M of boric acid, 9-12 mu M of manganese chloride tetrahydrate, 0.3-0.5 mu M of copper sulfate pentahydrate, 0.02-0.03 mu M of ammonium molybdate tetrahydrate and 5-20 mu M of zinc sulfate heptahydrate.
The calcium nitrate tetrahydrate provides nitrogen for the growth of wheat, the anhydrous potassium sulfate provides potassium, the monopotassium phosphate provides phosphorus and potassium, the magnesium sulfate heptahydrate provides magnesium, the disodium ferric ethylenediaminetetraacetate provides iron, the boric acid provides trace element boron, the manganese chloride tetrahydrate provides trace element manganese, the copper sulfate pentahydrate provides trace element copper, the ammonium molybdate tetrahydrate provides trace element molybdenum, the zinc sulfate heptahydrate provides zinc, and the sodium silicate nonahydrate provides silicon.
Then preparing the nutrient solution according to the following steps:
1): dissolving 4.45g of ferrous sulfate heptahydrate in 200ml of distilled water to obtain a ferrous sulfate heptahydrate solution, dissolving 5.96g of disodium ethylene diamine tetraacetate in 200ml of distilled water to obtain a disodium ethylene diamine tetraacetate solution, heating the disodium ethylene diamine tetraacetate solution by adopting a 70 ℃ water bath, slowly adding the ferrous sulfate heptahydrate solution into the disodium ethylene diamine tetraacetate while stirring, then chelating for 4 hours in a 70 ℃ oven, cooling to a constant volume of 1L, and storing in a first brown reagent bottle to obtain a mother solution A.
2): respectively preparing 1000 multiplied mother liquor B of calcium nitrate tetrahydrate, anhydrous potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate and zinc sulfate heptahydrate, metering the volume to 1L, and storing in a second brown reagent bottle.
3): absorbing 1mL of mother liquor A and mother liquor B respectively, dissolving in 999mL of deionized water, absorbing 0.5mL of mother liquor A and mother liquor B respectively, shaking up to obtain solution C.
4): and (4) adjusting the pH value of the solution C in the step (3) to 5.5-5.8, and storing in a third brown reagent bottle to obtain the nutrient solution.
EXAMPLE III
The embodiment provides a technical scheme: a nutrient solution formula for promoting wheat seedling growth and nitrogen absorption comprises the following components in percentage by concentration:
1.5-2.5mM of calcium nitrate tetrahydrate, 2.5-3mM of anhydrous potassium sulfate, 1-1.5mM of monopotassium phosphate, 2-2.5mM of magnesium sulfate heptahydrate, 100 mu M of disodium iron ethylenediaminetetraacetate, 46-55 mu M of boric acid, 9-12 mu M of manganese chloride tetrahydrate, 0.3-0.5 mu M of copper sulfate pentahydrate, 0.02-0.03 mu M of ammonium molybdate tetrahydrate and 10-20 mu M of sodium silicate nonahydrate.
The calcium nitrate tetrahydrate provides nitrogen for the growth of wheat, the anhydrous potassium sulfate provides potassium, the monopotassium phosphate provides phosphorus and potassium, the magnesium sulfate heptahydrate provides magnesium, the disodium ferric ethylenediaminetetraacetate provides iron, the boric acid provides trace element boron, the manganese chloride tetrahydrate provides trace element manganese, the copper sulfate pentahydrate provides trace element copper, the ammonium molybdate tetrahydrate provides trace element molybdenum, the zinc sulfate heptahydrate provides zinc, and the sodium silicate nonahydrate provides silicon.
Then preparing the nutrient solution according to the following steps:
1): dissolving 4.45g of ferrous sulfate heptahydrate in 200ml of distilled water to obtain a ferrous sulfate heptahydrate solution, dissolving 5.96g of disodium ethylene diamine tetraacetate in 200ml of distilled water to obtain a disodium ethylene diamine tetraacetate solution, heating the disodium ethylene diamine tetraacetate solution by adopting a 70 ℃ water bath, slowly adding the ferrous sulfate heptahydrate solution into the disodium ethylene diamine tetraacetate while stirring, then chelating for 4 hours in a 70 ℃ oven, cooling to a constant volume of 1L, and storing in a first brown reagent bottle to obtain a mother solution A.
2): respectively preparing 1000 multiplied mother liquor B of calcium nitrate tetrahydrate, anhydrous potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate and sodium silicate nonahydrate, metering the volume to 1L, and storing in a second brown reagent bottle.
3): absorbing 1mL of mother liquor A and mother liquor B respectively, dissolving in 999mL of deionized water, absorbing 0.5mL of mother liquor A and mother liquor B respectively, shaking up to obtain solution C.
4): adjusting the pH value of the solution C in the step 3 to 5.5-5.8, and storing in a third brown reagent bottle to obtain the nutrient solution
Experimental example 1
The nutrient solution of the experimental example adopts the nutrient solution provided by the first embodiment, a wheat variety Chinese spring is taken as an experimental material to carry out an indoor water culture experiment, two treatments are set by using the traditional wheat nutrient solution (Control) without adding zinc and silicon and the novel nutrient solution containing zinc and silicon (+ Zn + Si) of the invention, and root and overground part sampling is carried out when the seedling age of the wheat is three weeks to the end, so that the difference of biomass is analyzed. The results of the experiment are shown in FIG. 2.
Wherein, the growth conditions of wheat seedlings with traditional non-zinc and silicon added wheat nutrient solution (Control) and the novel zinc and silicon added nutrient solution (+ Zn + Si) of the invention are respectively shown in figure 2, the biomass of roots and overground parts are respectively shown in figure 2B and figure 2C, the Control shows the traditional non-zinc and silicon added wheat nutrient solution, and the + Zn + Si shows the novel zinc and silicon added nutrient solution of the invention.
The added zinc sulfate and sodium silicate can effectively improve the phenotype of the wheat planted indoors, and the biomass of the root system and the overground part is obviously improved, wherein the biomass of the root system is improved by 26.1 percent and the biomass of the overground part is improved by 32.4 percent compared with the biomass of the root system which is not added with zinc and silicon in the prior art. The new wheat seedling nutrient solution can effectively improve the phenotype of the wheat seedlings and increase the biomass.
Experimental example two
The nutrient solution of the experimental example adopts the nutrient solution provided by the first embodiment, the wheat variety of Chinese spring is taken as an experimental material to carry out indoor water culture experiment, and the traditional wheat without zinc and silicon is utilizedThe nutrient solution (Control) and the novel zinc and silicon containing nutrient solution (+ Zn + Si) are subjected to two treatments, root and overground part sampling is carried out when the seedling age of the wheat reaches three weeks, and differences of nitrogen accumulation, nitrate content, amino acid content and glutamine synthetase activity are analyzed. Experimental results referring to FIG. 3, Control represents a conventional wheat nutrient solution without adding zinc and silicon, + Zn + Si represents a novel zinc and silicon-containing nutrient solution of the present invention, FIG. 3A represents total nitrogen concentration of wheat roots and overground parts, and FIG. 3B represents NO3 -The concentration, fig. 3C, amino acid concentration (C), and fig. 3D, GS activity.
The zinc sulfate and the sodium silicate added in the invention can effectively increase the total nitrogen and the amino acid content of indoor planted wheat seedlings and improve the activity of glutamine synthetase. Compared with the traditional method without adding zinc and silicon, the total nitrogen accumulation of the overground part of the wheat is increased by 5.2% under the condition of the novel nutrient solution culture, although the nitrate accumulation is not obviously different, the amino acid accumulation is obviously improved, wherein the overground part is improved by 40.2%, and the root part is improved by 34.1%. Glutamine synthase plays an important role in nitrogen assimilation and utilization, and the activity of the glutamine synthase of the wheat seedlings is improved by 10.2 percent under the condition of novel nutrient solution culture. The novel wheat seedling nutrient solution can effectively improve the accumulation and utilization of nitrogen by wheat seedlings.
Experimental example III
The nutrient solution of the experimental example adopts the nutrient solution provided by the first embodiment, a wheat variety Chinese spring is taken as an experimental material to carry out an indoor water culture experiment, the traditional wheat nutrient solution (Control) without zinc and silicon and the novel nutrient solution containing zinc and silicon (+ Zn + Si) are used for setting two treatments, and Ca (Ca) (Si) with the same proportion is transferred to the wheat seedlings till the wheat seedlings are aged for three weeks15NO3)2Isotope treatment is carried out for 5min in the nutrient solution of the isotope, then roots and overground parts are collected, and the difference of the nitrogen absorption rate is analyzed. The experimental results are shown in FIG. 4, wherein Control represents the traditional wheat nutrient solution without adding zinc and silicon, and + Zn + Si represents the novel nutrient solution containing zinc and silicon.
The zinc sulfate and the sodium silicate added in the invention can effectively improve the nitrogen absorption rate to 39%. This shows that the novel wheat seedling nutrient solution of the invention can effectively increase the nitrogen absorption rate of wheat seedlings, and the significant difference between the wheat biomass under different nutrient solution conditions is indicated by "+".
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A nutrient solution formula for promoting wheat seedling growth and nitrogen absorption is characterized in that: the formula comprises the following components:
calcium nitrate tetrahydrate, anhydrous potassium sulfate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, disodium iron ethylenediaminetetraacetate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, zinc sulfate heptahydrate and sodium silicate nonahydrate.
2. The nutrient solution formula for promoting wheat seedling growth and nitrogen absorption as claimed in claim 1, wherein the nutrient solution formula comprises: the formula comprises the following components in percentage by concentration:
1.5-2.5mM of calcium nitrate tetrahydrate, 2.5-3mM of anhydrous potassium sulfate, 1-1.5mM of monopotassium phosphate, 2-2.5mM of magnesium sulfate heptahydrate, 100 mu M of disodium iron ethylenediaminetetraacetate, 46-55 mu M of boric acid, 9-12 mu M of manganese chloride tetrahydrate, 0.3-0.5 mu M of copper sulfate pentahydrate, 0.02-0.03 mu M of ammonium molybdate tetrahydrate, 5-20 mu M of zinc sulfate heptahydrate and 10-20 mu M of sodium silicate nonahydrate.
3. A method for preparing a nutrient solution for promoting wheat seedling growth and nitrogen absorption, which is suitable for the nutrient solution formula for promoting wheat seedling growth and nitrogen absorption of any one of claims 1 to 2, and is characterized in that: the method comprises the following steps:
1): dissolving 4.45g of ferrous sulfate heptahydrate in 200ml of distilled water to obtain a ferrous sulfate heptahydrate solution, dissolving 5.96g of disodium ethylene diamine tetraacetate in 200ml of distilled water to obtain a disodium ethylene diamine tetraacetate solution, heating the disodium ethylene diamine tetraacetate solution, slowly adding the ferrous sulfate heptahydrate solution into the disodium ethylene diamine tetraacetate while stirring, then chelating in an oven, cooling to a constant volume of 1L, and storing in a first reagent bottle to obtain a mother solution A;
2): respectively preparing 1000 multiplied mother liquor B of calcium nitrate tetrahydrate, anhydrous potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, zinc sulfate heptahydrate and sodium silicate nonahydrate, fixing the volume to 1L, and storing in a second reagent bottle;
3): respectively absorbing 1mL of mother liquor A and 1mL of mother liquor B, dissolving in 999mL of deionized water, shaking up to obtain a solution C;
4): and (4) storing the solution C obtained in the step (3) in a third reagent bottle to obtain the nutrient solution.
4. The method for preparing the nutrient solution for promoting the growth of wheat seedlings and the absorption of nitrogen as claimed in claim 3, wherein the nutrient solution comprises the following components: the pH value of the solution C is 5.5-5.8.
5. The method for preparing the nutrient solution for promoting the growth of wheat seedlings and the absorption of nitrogen as claimed in claim 3, wherein the nutrient solution comprises the following components: in the step 1, the heating temperature of the disodium ethylene diamine tetraacetate solution is 70 ℃.
6. The method for preparing the nutrient solution for promoting the growth of wheat seedlings and the absorption of nitrogen as claimed in claim 3, wherein the nutrient solution comprises the following components: the first reagent bottle, the second reagent bottle and the third reagent bottle are brown reagent bottles.
7. The method for preparing the nutrient solution formula for promoting the growth of wheat seedlings and the absorption of nitrogen as claimed in claim 3, wherein the nutrient solution formula comprises the following components: the temperature of the oven in step 1 was 70 degrees celsius and the chelation time was 4 hours.
8. The method for preparing the nutrient solution formula for promoting the growth of wheat seedlings and the absorption of nitrogen as claimed in claim 3, wherein the nutrient solution formula comprises the following components: in step 3, 0.5ml of each of the mother liquor A and the mother liquor B was taken.
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