CN110668664B - Microbial agent filler for environment-friendly ecological toilet and preparation method thereof - Google Patents
Microbial agent filler for environment-friendly ecological toilet and preparation method thereof Download PDFInfo
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- C02F11/00—Treatment of sludge; Devices therefor
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- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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Abstract
The invention relates to a microbial agent filler for an environment-friendly ecological toilet, which comprises the following strains: the filler is prepared by mixing penicillium, streptomycete, saccharomycetes, lactic acid bacteria, cyanobacteria, bacillus, pseudomonas, trichoderma, rhodococcus and thioreduction bacteria with a sterilized filler base material containing sawdust, rice hulls, peanut shells and corn starch after the microbial agent is cultured. The microbial inoculum filler is used for harmless treatment of excrement in a microbial environment-friendly ecological toilet, and a degraded product is used as an organic fertilizer.
Description
Technical Field
The invention belongs to the technical field of environmental microbiology, and particularly relates to a microbial inoculum formula for degrading fecal sewage of a microbial ecological environment-friendly toilet, a filler and a preparation method thereof.
Background
The microorganism ecological environment-friendly toilet attracts wide attention due to the advantages of no water flushing and capability of treating feces on site. The microorganism in the microbial ecological toilet filler can degrade macromolecules in the excrement into micromolecules and convert the micromolecules into bacteria and gas, inhibit and kill harmful microorganisms in the excrement, adsorb, degrade and convert odor substances generated in the excrement, and meet the requirements of on-site harmless and quantitative reduction treatment on the excrement. But the microbial agents aiming at the microbial ecological toilet in the market are less, the excrement degradation efficiency is still not high, and the effective life of the microbial agents is shorter. The livestock and poultry manure fermentation bed has the defects of poor air permeability, short storage time after production, short service life, poor evaporation effect on water in urine and easy formation of anaerobic fermentation in the application of a microbial ecological environment-friendly toilet, so that the treatment effect on manure and odor is poor. For example, the Chinese patent application of 'a compound microbial agent for toilets and application thereof' (publication No. CN108865943A) and 'a microbial zymocyte for rapidly deodorizing and fixing nitrogen and treating human and animal excreta' (publication No. CN108486003A) have the problems.
Disclosure of Invention
Aiming at the technical problems, the invention aims to provide a filler containing a microbial ecological environment-friendly toilet excrement degrading microbial inoculum and a preparation method thereof, and the filler can effectively solve the problems of poor excrement degrading effect, poor odor treatment effect and short acting time.
In order to achieve the purpose, the invention provides the following technical scheme:
the microbial agent filler for the environment-friendly ecological toilet comprises the following strains: the filler is prepared by mixing penicillium, streptomycete, saccharomycetes, lactic acid bacteria, cyanobacteria, bacillus, pseudomonas, trichoderma, rhodococcus and thioreduction bacteria with a sterilized filler base material containing sawdust, rice hulls, peanut shells and corn starch after the microbial agent is cultured.
The microbial agent in the filler is used for degrading the toilet excrement by microorganisms, so that the harmless treatment of the toilet excrement is realized, organic matters in the excrement are converted into water, carbon dioxide and the like, the content of harmful microorganisms such as escherichia coli and the like in the excrement is reduced by 5-90%, and the generation of odor is reduced.
The microbial agent comprises the following strains:
the penicillium comprises penicillium funiculosum and penicillium polytropic;
streptomyces include streptomyces donaldii;
the yeast includes Cryptococcus laurentii and Candida krusei;
the lactobacillus includes Pediococcus acidilactici and Lactobacillus acidophilus;
cyanobacteria include Ficoll blue bacteria;
the Bacillus comprises Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus lysinibacillus globiformis, Bacillus brevis, Bacillus cereus, Bacillus halophilus and Bacillus thermotolerant;
the sulfur-reducing bacteria include sulfobacillus thermosulfidooxidans;
the Pseudomonas comprises Pseudomonas fluorescens and Rhodopseudomonas palustris;
the Trichoderma comprises Trichoderma koningii;
the Rhodococcus includes Rhodococcus erythropolis.
The microbial agent comprises the following strains in percentage by weight: 1-3 parts of penicillium funiculosum powder, 1-3 parts of polytropic penicillium funiculosum powder, 1-3 parts of streptomyces donaldii powder, 1-3 parts of cryptococcus laurentii powder, 1-3 parts of candida krusei powder, 1-3 parts of pediococcus acidilactici powder, 1-3 parts of cyanobacteria powder, 1-3 parts of bacillus subtilis powder and 1-3 parts of bacillus amyloliquefaciens powder,1-3 parts of spherical lysine bacillus powder, 1-3 parts of Brevibacillus brevis powder, 1-3 parts of Bacillus cereus powder, 1-3 parts of Lactobacillus acidophilus powder, 1-3 parts of Bacillus halophilus powder, 1-3 parts of heat-resistant bacillus powder, 1-3 parts of sulfide reducing bacteria powder, 1-3 parts of fluorescent pseudomonas powder, 1-3 parts of Rhodopseudomonas palustris powder, 1-3 parts of Trichoderma koningii powder and 1-3 parts of Rhodococcus erythropolis powder; wherein the activity of each bacterial powder is 106-1010cfu/g。
The bottom material of the filler is prepared from sawdust, rice hulls, peanut shells, corn starch, bean pulp, fly ash and water; the contents of the components by weight ratio are as follows: 200 parts of sawdust, 130 parts of rice husk, 10-30 parts of peanut shell, 10-30 parts of corn starch, 5-10 parts of bean pulp and 10-25 parts of fly ash, adjusting the water content to 20-70%, and sterilizing for 10-60min at 220 ℃ by using a sterilization kettle 110.
The mixing ratio of the microbial inoculum to the filler base material is 1-10: 10000.
The replacement period of the filler is 1-12 months, and the storage life is 1-4 months.
A preparation method of a microbial agent filler for an environment-friendly ecological toilet comprises the following steps:
1) the following strains were: mixing Penicillium, Streptomyces, yeast, lactic acid bacteria, cyanobacteria, Bacillus, Pseudomonas, Trichoderma, Rhodococcus and sulfide reducing bacteria, and culturing to obtain microbial agent;
2) mixing the sterilized raw materials containing sawdust, rice hulls, peanut shells and corn starch, adjusting the water content to be 20-70%, and sterilizing for 15-30min at 220 ℃ by using a sterilization kettle 180-;
3) and uniformly stirring the microbial inoculum and the filler base material according to the mixing ratio of the microbial inoculum to the filler base material of 1-10:10000, uniformly stirring once every 2-6 hours, and performing pre-fermentation for 24-72 hours to obtain the microbial inoculum filler for the ecological environment-friendly toilet.
Compared with the prior art, the invention has the beneficial effects that:
the microbial-containing ecological toilet excrement degrading microbial inoculum filler can effectively degrade excrement, and the efficiency of degrading the toilet excrement is 60-95%; but also can convert and absorb the odor gases such as ammonia gas, hydrogen sulfide, indole and the like in the excrement, so that the emission of related gases is reduced by more than 10 to 95 percent, the replacement period of the filler is 1 to 12 months, and the storage life is 1 to 4 months.
Detailed Description
The present invention will be further illustrated with reference to the following examples.
The filler contains a microbial ecological toilet excrement degrading microbial inoculum, and the microbial inoculum comprises the raw materials of penicillium funiculosum, variegated penicillium, streptomyces thalidomii, cryptococcus laurentii, candida krusei, pediococcus acidilactici, cyanobacteria fischeri, bacillus subtilis, bacillus amyloliquefaciens, bacillus lysinibacillus globiformis, bacillus brevis, bacillus cereus, lactobacillus acidophilus, bacillus halophilus, bacillus thermotolerant bacillus, thiobacillus thiothermalis, pseudomonas fluorescens, rhodopseudomonas palustris, trichoderma koningii and rhodococcus erythropolis.
Preferably, the microbial inoculum comprises 1-3 parts of penicillium funiculosum powder, 1-3 parts of variegated penicillium funiculosum powder, 1-3 parts of streptomyces thalictri powder, 1-3 parts of cryptococcus laurentii powder, 1-3 parts of candida krusei powder, 1-3 parts of pediococcus acidilactici powder, 1-3 parts of cyanobacteria powder, 1-3 parts of bacillus subtilis powder, 1-3 parts of bacillus amyloliquefaciens powder, 1-3 parts of lysine sphaericus powder, 1-3 parts of brevibacillus brevis powder, 1-3 parts of bacillus cereus powder, 1-3 parts of lactobacillus acidophilus powder, 1-3 parts of bacillus halophilus powder, 1-3 parts of bacillus thermotolerant bacillus powder, 1-3 parts of thiobacillus thermophilus, 1-3 parts of pseudomonas fluorescens powder, 1-3 parts of rhodopseudomonas palustris powder, 1-3 parts of trichoderma koningii powder and 1-3 parts of rhodococcus erythropolis powder.
Preferably, the vitality of each bacterial powder is 106-1010cfu/g。
Weighing raw materials for preparing the microbial toilet filler, wherein the filler base material is prepared from sawdust, rice hulls, peanut shells, corn starch, bean pulp, fly ash and water. Wherein, the water content is adjusted to be 20-70 percent by using 150 portions of sawdust, 130 portions of rice husk, 10-30 portions of peanut shell, 10-30 portions of corn starch, 5-10 portions of bean pulp and 10-25 portions of fly ash, and the materials are sterilized for 15-30min at 220 ℃ by using a sterilization kettle 180-.
Uniformly stirring the mixture ratio of the microbial inoculum to the filler base material of 1-10: 10000. Stirring and mixing uniformly once every 2-6 hours, and adding a microbial toilet as a filler after pre-fermentation is carried out for 24-72 hours.
The main microbial inoculum raw materials of the fecal sewage degrading microorganism provided by the invention are easy to obtain, and the main microbial inoculum comprises common microorganisms such as bacillus subtilis, brevibacillus brevis, penicillium, saccharomycetes and the like, and has strong environmental adaptability and long service life. Fly ash provides inorganic salts necessary for the growth of microorganisms.
Preferably, the microbial inoculum raw materials comprise, by weight, 7.5g of penicillium funiculosum powder, 7.5g of variegated penicillium funiculosum powder, 7.5g of streptomyces thalictri powder, 7.5g of cryptococcus laurentii powder, 7.5g of candida krusei powder, 7.5g of pediococcus acidilactici powder, 7.5g of cyanobacteria powder, 7.5g of bacillus subtilis powder, 2.5g of bacillus amyloliquefaciens powder, 2.5g of lysine sphaericus powder, 7.5g of brevibacillus brevis powder, 2.5g of bacillus cereus powder, 2.5g of lactobacillus acidophilus powder, 7.5g of bacillus halophilus powder, 2.5g of bacillus thermotolerant bacillus bacteria powder, 2.5g of sulfide reducing bacteria powder, 2.5g of pseudomonas fluorescens powder, 2.5g of rhodopseudomonas palustris powder, 2.5g of trichoderma koningii powder and 2.5g of rhodococcus erythropolis powder.
Wherein the activity of the strain powder of the penicillium funiculosum is 1.7 multiplied by 108cfu/g, the activity of the variable penicillium powder is 1.2 multiplied by 108cfu/g, the thallus activity of the streptomyces dongdahlii bacterial powder is 3.3 multiplied by 106cfu/g, the thallus activity of the cryptococcus laurentii powder is 3.5 multiplied by 108cfu/g, the bacterial activity of the krusei strain powder is 2.9 multiplied by 108cfu/g, the thallus activity of the pediococcus acidilactici powder is 6.7 multiplied by 109cfu/g, the bacterial viability of the powder of the cyanobacteria freudenreichii is 1.9 multiplied by 106cfu/g, the bacterial activity of the bacillus subtilis powder is 3.6 multiplied by 109cfu/g, the activity of the bacillus amyloliquefaciens powder is 6.6 multiplied by 109cfu/g, the thallus activity of the spherical lysine bacillus powder is 3.2 multiplied by 109cfu/g, the activity of the Brevibacillus brevis powder is 3.7 multiplied by 109cfu/g, bacillus cereus powder thallusThe activity was 3.5X 108cfu/g, the activity of Lactobacillus acidophilus powder is 2.2 multiplied by 108cfu/g, the activity of the bacillus halophilus powder is 1.1 multiplied by 107cfu/g, the activity of the heat-resistant bacillus powder is 1.3 multiplied by 106cfu/g, the activity of the thermophilic sulfur oxidation thiobacillus powder is 1.1 multiplied by 106cfu/g, the bacterial activity of the fluorescent pseudomonas powder is 1.1 multiplied by 106cfu/g, the thallus activity of rhodopseudomonas palustris powder is 1.1 multiplied by 102cfu/g, the viability of the Trichoderma koningii powder is 1.2 multiplied by 106cfu/g, the thallus activity of the Rhodococcus erythropolis powder is 1.3 multiplied by 106cfu/g。
Weighing a base material for preparing the microbial toilet filler, wherein the base material comprises: 40kg of sawdust, 13kg of rice hull, 2kg of peanut shell, 2kg of corn starch, 5kg of soybean meal and 15kg of fly ash, adjusting the water content to 40%, and sterilizing for 30min at 120 ℃ by using a sterilization kettle.
The microbial inoculum and the filler backing material are mixed evenly by weight. Stirring and mixing the mixture once every 3 hours, adding a microbial toilet as a filler after pre-fermentation is carried out for 24 hours, and the degradation efficiency of the excrement in the toilet is 60-95%.
Claims (3)
1. The microbial agent filler for the environment-friendly ecological toilet is characterized in that: the microbial agent comprises the following strains: the filler is prepared by mixing penicillium, streptomycete, saccharomycetes, lactic acid bacteria, cyanobacteria, bacillus, pseudomonas, trichoderma, rhodococcus and thioreduction bacteria with a sterilized filler base material containing sawdust, rice hulls, peanut shells and corn starch after the microbial agent is cultured;
the penicillium comprises penicillium funiculosum and penicillium polytropic;
streptomyces include streptomyces donaldii;
the yeast includes Cryptococcus laurentii and Candida krusei;
the lactobacillus includes Pediococcus acidilactici and Lactobacillus acidophilus;
cyanobacteria include Ficoll blue bacteria;
the Bacillus comprises Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus lysinibacillus globiformis, Bacillus brevis, Bacillus cereus, Bacillus halophilus and Bacillus thermotolerant;
the sulfur-reducing bacteria include sulfobacillus thermosulfidooxidans;
the Pseudomonas comprises Pseudomonas fluorescens and Rhodopseudomonas palustris;
the Trichoderma comprises Trichoderma koningii;
the Rhodococcus includes Rhodococcus erythropolis;
the microbial agent comprises the following strains in percentage by weight: 1-3 parts of penicillium funiculosum powder, 1-3 parts of polytropic penicillium bacteria powder, 1-3 parts of streptomyces donaldii bacteria powder, 1-3 parts of cryptococcus laurentii powder, 1-3 parts of candida krusei powder, 1-3 parts of pediococcus acidilactici powder, 1-3 parts of cyanobacteria powder, 1-3 parts of bacillus subtilis powder, 1-3 parts of bacillus amyloliquefaciens powder, 1-3 parts of lysine bacillus sphaericus powder, 1-3 parts of brevibacillus brevis powder, 1-3 parts of bacillus cereus powder, 1-3 parts of lactobacillus acidophilus powder, 1-3 parts of bacillus halophilus powder, 1-3 parts of heat-resistant bacillus bacteria powder, 1-3 parts of sulfur reducing bacteria powder, 1-3 parts of pseudomonas fluorescens bacteria powder and 1-3 parts of rhodopseudomonas palustris powder, 1-3 parts of trichoderma koningii powder and 1-3 parts of rhodococcus erythropolis powder; wherein the activity of each bacterial powder is 106-1010 cfu/g；
The bottom material of the filler is prepared from sawdust, rice hulls, peanut shells, corn starch, bean pulp, fly ash and water; the contents of the components by weight ratio are as follows: 200 parts of sawdust, 130 parts of rice husk, 10-30 parts of peanut shell, 10-30 parts of corn starch, 5-10 parts of bean pulp and 10-25 parts of fly ash, adjusting the water content to 20-70%, and sterilizing for 10-60min at 220 ℃ by using a sterilization kettle 110;
the mixing ratio of the microbial inoculum to the filler base material is 1-10: 10000;
the replacement period of the filler is 1-12 months, and the storage life is 1-4 months.
2. The microbial agent filler for the eco-friendly ecological toilet according to claim 1, wherein: the microbial agent in the filler is used for degrading the toilet excrement by microorganisms, so that the harmless treatment of the toilet excrement is realized, organic matters in the excrement are converted into water and carbon dioxide, the content of harmful microorganisms of escherichia coli in the excrement is reduced by 5-90%, and the generation of odor is reduced.
3. The method for preparing the microbial agent filler for the eco-friendly toilet as claimed in claim 1, wherein: the method comprises the following steps:
1) the following strains were: mixing Penicillium, Streptomyces, yeast, lactic acid bacteria, cyanobacteria, Bacillus, Pseudomonas, Trichoderma, Rhodococcus and sulfide reducing bacteria, and culturing to obtain microbial agent;
2) mixing the sterilized raw materials containing sawdust, rice hulls, peanut shells and corn starch, adjusting the water content to be 20-70%, and sterilizing for 15-30min at 220 ℃ by using a sterilization kettle 180-;
3) and uniformly stirring the microbial inoculum and the filler base material according to the mixing ratio of the microbial inoculum to the filler base material of 1-10:10000, uniformly stirring once every 2-6 hours, and performing pre-fermentation for 24-72 hours to obtain the microbial inoculum filler for the ecological environment-friendly toilet.
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