CN110656047A - Cell culture dish bacterial infection experimental apparatus - Google Patents
Cell culture dish bacterial infection experimental apparatus Download PDFInfo
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- CN110656047A CN110656047A CN201910974748.4A CN201910974748A CN110656047A CN 110656047 A CN110656047 A CN 110656047A CN 201910974748 A CN201910974748 A CN 201910974748A CN 110656047 A CN110656047 A CN 110656047A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 77
- 208000035143 Bacterial infection Diseases 0.000 title claims abstract description 39
- 208000022362 bacterial infectious disease Diseases 0.000 title claims abstract description 39
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 230000001580 bacterial effect Effects 0.000 claims abstract description 39
- 239000007789 gas Substances 0.000 claims abstract description 20
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 19
- 230000000249 desinfective effect Effects 0.000 claims abstract description 4
- 238000007599 discharging Methods 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 239000002912 waste gas Substances 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 108
- 238000004140 cleaning Methods 0.000 claims description 15
- 230000001133 acceleration Effects 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 208000015181 infectious disease Diseases 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000000737 periodic effect Effects 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 description 14
- 238000000034 method Methods 0.000 description 9
- 230000006872 improvement Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000000889 atomisation Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
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- 239000011435 rock Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Abstract
The invention discloses a cell culture dish bacterial infection experimental device which comprises a liquid inlet module, a gas inlet module, a cell culture module, a gas outlet module, a disinfection module and a control module, wherein the liquid inlet module is connected with the gas inlet module; the air inlet module is used for entering external air; the liquid inlet module is used for conveying a bacterial culture solution to the cell culture module for cell culture; the air outlet module is used for discharging waste gas; the disinfection module is used for disinfecting the cell culture module, and the control module is used for controlling the liquid inlet module, the gas inlet module, the cell culture module, the gas outlet module and the disinfection module; the cell culture module comprises a cabin body capable of being controlled in a sealing mode, the cabin body is divided into a first cabin, a second cabin and a third cabin, the first cabin is provided with a disc culture frame, the second cabin is provided with a linear culture frame, and the third cabin is provided with a blank group frame. The invention can perform cell culture intuitively and fully automatically through multiple groups of controls, and improves the convenience and accuracy of experiments.
Description
Technical Field
The invention relates to the field of cell culture, in particular to a cell culture dish bacterial infection experimental device.
Background
The culture dish is a common laboratory ware, be used for microorganism or cell culture often, current culture dish is generally as a bearing device, the operation is operated manually completely, need the experimenter to carry out the experiment repeatedly, operate many times, and will carry out strict washing and sterilization after using at every turn, but often the experimenter is very heavy in experimental task every day, when carrying out cell culture, receive the pollution of air easily, cause the uncertain factor of cell culture to appear often, it is unsuccessful to cause cell culture, extravagant experimental material, it can't continue to go on to lead to follow-up experiment then seriously, can't obtain the experimental result of wanting.
When carrying out artifical liquid feeding, can appear that the liquid feeding is inhomogeneous in current cell culture, the contrast effect of bacteria culture is not obvious, urgently needs one kind can improve the experimental result accuracy, operates conveniently, can synthesize the cell culture dish experimental apparatus of contrast to alleviate experimenter's burden.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a full-automatic cell culture experimental device.
The invention provides a cell culture dish bacterial infection experimental device which is supported by a bracket and comprises: the device comprises a liquid inlet module, a gas inlet module, a cell culture module, a gas outlet module, a disinfection module and a control module; the air inlet module is used for entering external air; the liquid inlet module is used for conveying a bacterial culture solution to the cell culture module for cell culture; the air outlet module is used for discharging waste gas; the disinfection module is used for disinfecting the cell culture module, and the control module is used for controlling the liquid inlet module, the gas inlet module, the cell culture module, the gas outlet module and the disinfection module; the cell culture module comprises a cabin body capable of being controlled in a sealing mode, the cabin body is divided into a first cabin, a second cabin and a third cabin, the first cabin is provided with a disc culture frame, the second cabin is provided with a linear culture frame, and the third cabin is provided with a blank group frame.
The cell culture dish bacterial infection experimental device is characterized in that the first chamber is a natural exposure infection chamber, the disc culture frame comprises a plurality of culture medium fixing positions, the culture medium fixing positions are uniformly distributed along a circular ring in the same circle center and used for fixing a culture medium, a culture medium inlet module for allowing a culture medium to enter is arranged on the side surface of the disc culture base frame, and the culture medium inlet module is used for conveying the culture medium to the disc culture frame and a linear culture frame of the second chamber respectively;
the disc is cultivateed frame upside and is set up the fixed position of a plurality of culture mediums, the downside of every fixed position of the fixed position of a plurality of culture mediums all sets up first temperature control module, and first temperature control module downside sets up supporting baseplate, the supporting baseplate downside supports through the axis of rotation.
The cell culture dish bacterial infection experimental device is characterized in that the edge of the outermost periphery of the culture medium fixing position is a first periphery, the edge of the outermost periphery of the support bottom plate is a second periphery, the first periphery is smaller than the second periphery, at least four springs are arranged between the first periphery and the second periphery at equal angular intervals, and the support bottom plate is driven by the rotation of the rotating shaft to drive the culture medium fixing position to move in each elastic direction of the springs so as to uniformly mix a culture medium and a bacterial culture solution;
the upper side of the support bottom plate is further provided with a bulge which is in the same direction as the spring, the bulge is of a T-shaped structure, the lower side of the culture medium fixing position is further provided with a groove which protrudes downwards and is in the same direction as the spring, the groove forms an inverted T shape with a hollow inner part through the support arm and is matched with the bulge, and the bulge is coupled with the groove, so that when the support bottom plate rotates, the culture medium fixing position only moves in the elastic direction of the spring and does not move in the vertical direction, and the spring does not deflect in the inelastic direction.
The cell culture dish bacterial infection experimental apparatus, temperature control module receives control module's control signal, carries out temperature control, temperature control module sets up under every culture medium, and not with the recess overlaps.
Cell culture dish bacterial infection experimental apparatus, the axis of rotation drives through first driving motor, first driving motor includes two periodic operating condition, preceding half cycle, first driving motor evenly rotates, latter half cycle, the inhomogeneous rotation of first driving motor, through inhomogeneous rotation, make the spring under the effect of different eccentric forces, control different spring productions of control, control in the elasticity direction the culture medium is fixed to be removed to the production rocks the action and makes bacterial culture solution and culture medium mixing.
The cell culture dish bacterial infection experimental device is characterized in that the uneven rotation comprises acceleration or deceleration rotation through set acceleration, change of rotation speed to form fast and slow rotation, and stop of circular rotation after rotation.
In the cell culture dish bacterial infection experimental device, the second chamber is a quantitative culture chamber, the linear culture frame is in a linear structure, the first end part of the linear structure is connected with the culture medium inlet module, and the culture medium inlet module is perpendicular to the linear culture frame; the upper side of the linear culture frame stretches across a portal frame, the portal frame is provided with a liquid feeding needle which can move along a cross bar on the upper side of the portal frame, one side of the linear culture frame is also provided with a cleaning module and a concentration adjusting module which are vertical to the linear culture frame, the cleaning module and the concentration adjusting module are both arranged on the same side of the linear culture frame as the culture medium inlet module, and the cleaning module and the concentration adjusting module are arranged right below the portal frame;
liquid feeding needle passes through the pipe connection the feed liquor module will bacterial culture solution in the feed liquor module adds earlier in the concentration adjustment module, through concentration adjustment module is according to different concentration partial shipment in different containers, through portal frame control the liquid feeding needle does not take a sample in proper order and need the bacterial culture solution of different concentrations in the container, adds the bacterial culture solution and gets into in the culture medium in the straight line culture frame, when the bacterial culture solution of different concentrations of taking a sample at every turn, deepens the liquid feeding needle and washs in the cleaning module.
Cell culture dish bacterial infection experimental apparatus, the linear culture frame sets up a plurality of positions of holding, the linear culture frame still includes linear guide, driving band, the driving band drive hold the position and follow linear guide carries out the perpendicular to the removal of portal frame upside horizontal pole to make every culture medium move and carry out the liquid feeding under the portal frame.
The cell culture dish bacterial infection experimental device is characterized in that a mark module is arranged at an inlet of the first end part, the mark module marks when a culture medium enters a containing position of the linear culture frame, a mark reading module is arranged on the side edge of a linear guide rail of the linear culture frame facing the portal frame, when the culture medium moves to the lower part of the portal frame, the mark reading module reads a first mark of the culture medium positioned right below the portal frame at the moment, the read first mark is sent to a control module, the control module controls the liquid adding needle to add a bacterial culture solution with a first concentration into a culture with the first mark after receiving the first mark, associates and stores the first concentration and the first mark, and associates and stores the culture medium with different concentrations after corresponding liquid adding according to different marks and bacterial culture solutions with different concentrations in sequence, only clear water was added to the last medium as a blank.
Cell culture dish bacterial infection experimental apparatus set up second temperature control module in the second bin, second temperature control module receives control module's temperature carries out temperature control, control module sends same temperature information control first temperature control module and second temperature control module at first and carries out same temperature control, then, continuously monitors the first temperature of first temperature control module and the second temperature of first bin, compares the difference of first temperature and second temperature, works as when the difference exceeds the temperature threshold, adjusts first temperature control module and carries out temperature adjustment to adjust the adjustment according to temperature adjustment control second temperature control module adjustment temperature.
The beneficial technical effects obtained by the invention are as follows: the invention can carry out a plurality of experimental controls during cell culture, can operate in a full-automatic mode, is convenient and intuitive to observe experimental variables, and can conveniently and accurately obtain the experimental result of cell culture in a natural non-quantitative and accurate quantitative mode by automatic culture medium temperature regulation, liquid adding and sampling. The invention has the main improvement points that two different culture platforms are arranged aiming at two different bacterial infection modes, natural infection is simulated by a disc mode to culture cells, bacterial culture solution is fully mixed by the rotation of the disc, quantitative liquid adding is carried out by a linear mode, and the volume of an experimental device is saved. The other improvement mode of the invention is that the diameters of the support frame and the culture medium fixing position are different, the centrifugal force of the rotation of the disc is utilized to be matched with the spring, so that the culture medium fixing position moves on the support frame, bacteria in a natural state are fully absorbed when the disc rotates, and the bacteria are fully mixed under the matching of the spring. The invention has the improvement points that the marking information and the bacteria culture solution with different concentrations can be used for marking, the control module can be conveniently identified, and the linear guide rail is arranged, and particularly, the entering module and the cleaning module at the periphery of the linear guide rail are vertical to the linear guide rail.
Drawings
The invention will be further understood from the following description in conjunction with the accompanying drawings. The components in the figures are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the embodiments. In the drawings, like reference numerals designate corresponding parts throughout the different views.
FIG. 1 is a schematic view of the bacterial infection experiment apparatus of the cell culture dish of the present invention.
FIG. 2 is a control block diagram of the experimental apparatus of the present invention.
FIG. 3 is a schematic diagram of the operation method of the bacterial infection experimental device of the cell culture dish.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments thereof; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Other systems, methods, and/or features of the present embodiments will become apparent to those skilled in the art upon review of the following detailed description. It is intended that all such additional systems, methods, features and advantages be included within this description, be within the scope of the invention, and be protected by the accompanying claims. Additional features of the disclosed embodiments are described in, and will be apparent from, the detailed description that follows.
As shown in figure 1, the invention provides a cell culture dish bacterial infection experimental device, which comprises an air inlet module 1, an atomization module 2, a cell culture module shell 3, a support 4 of a third chamber, a third chamber 5, a base 6, wheels 7, an air outlet pipe 8, a filter screen 9, a second chamber 10, a liquid inlet pipeline 11 entering the second chamber 10, a culture medium entering module 12, a culture medium entering module 13 entering the second chamber, a linear culture frame 14, a groove 15, a spring 16, a bacterial culture bottle 17, a liquid inlet pipeline 18 entering the atomization module, a rotating shaft 19, a support bottom plate 20, a culture medium fixing position 21, a first section of culture medium flow channel 22, a second section of culture medium flow channel 23 and a chamber door 24.
Support by a support, comprising: the device comprises a liquid inlet module, a gas inlet module, a cell culture module, a gas outlet module, a disinfection module and a control module; the air inlet module is used for entering external air; the liquid inlet module is used for conveying a bacterial culture solution to the cell culture module for cell culture; the air outlet module is used for discharging waste gas; the disinfection module is used for disinfecting the cell culture module, and the control module is used for controlling the liquid inlet module, the gas inlet module, the cell culture module, the gas outlet module and the disinfection module; the cell culture module comprises a cabin body capable of being controlled in a sealing mode, the cabin body is divided into a first cabin, a second cabin and a third cabin, the first cabin is provided with a disc culture frame, the second cabin is provided with a linear culture frame, and the third cabin is provided with a blank group frame.
Preferably, the liquid inlet module, the air inlet module and the air outlet module respectively comprise a valve controlled by the receiving control module, and the disinfection module receives a control signal of the receiving control module and starts disinfection.
Preferably, the control module controls the sterilization module to sterilize the inside of the chamber before the culture medium and the bacteria culture solution are introduced into the chamber.
Preferably, the feed liquor module and the module of admitting air get into the back through different pipelines, carry out the pressurization atomizing through the force (forcing) pump, spray the bacterial culture solution after the atomizing in first bin when the bacterial culture solution sprays, the first driving motor of control module control is carried out and is rotated according to the cycle. The liquid inlet module is divided into two outlets, one outlet is connected with the inlet module, and the other outlet is connected to the liquid adding needle of the second chamber. Before the pressurization pump atomizes, the other outlet is controlled to add the bacteria culture solution into the concentration adjusting module of the second chamber through the liquid adding needle for concentration adjustment, the bacteria culture solution is subpackaged into different containers, and after the concentration adjustment is finished, pressurization atomization is carried out.
Preferably, the module of admitting air all sets up the filter screen with the module of giving vent to anger, it includes first delivery outlet and second delivery outlet to get into the module, first delivery outlet is connected the feed liquor module, the second bin is connected to the second delivery outlet, the module of giving vent to anger sets up the filter screen for the gas outgoing after will filtering.
The cell culture dish bacterial infection experimental device is characterized in that the first chamber is a natural exposure infection chamber, the disc culture frame comprises a plurality of culture medium fixing positions, the culture medium fixing positions are uniformly distributed along a circular ring in the same circle center and used for fixing a culture medium, a culture medium inlet module for allowing a culture medium to enter is arranged on the side surface of the disc culture base frame, and the culture medium inlet module is used for conveying the culture medium to the disc culture frame and a linear culture frame of the second chamber respectively;
the disc is cultivateed frame upside and is set up the fixed position of a plurality of culture mediums, the downside of every fixed position of the fixed position of a plurality of culture mediums all sets up first temperature control module, and first temperature control module downside sets up supporting baseplate, the supporting baseplate downside supports through the axis of rotation.
Preferably, the third chamber is a medium-containing chamber to which no bacteria culture medium is added, and preferably, clear water may be added. Set up culture medium flow channel between third bin and the first bin, when the disc culture frame of first bin got into a plurality of culture mediums, can rotate and remove empty culture medium fixed position to culture medium entering module in proper order, when rotatory, will have the culture medium fixed position of culture medium to remove and be directed against culture medium flow channel, then control module control culture medium flow channel opens, circulates third bin with one of them culture medium. The medium flow path is then closed, isolating the third compartment from the first compartment. The culture medium flow channel can be provided with rollers at the bottom, is pushed from inside to outside through a culture medium fixing position, rolls on the rollers and enters the support of the third chamber, and can also be actively driven by a driving belt to enter the third chamber.
Preferably, the medium flow path is divided into two sections, one in the first chamber and the other in the third chamber, and a gap of a certain width is provided between the two sections, through which a gate can be closed or opened, and the gap can be spanned by driving of rollers or driving belts, and substantially, the gap is very small as long as the gate can be accommodated.
Preferably, the culture medium is a liquid culture medium contained in a fixed box, and the culture medium refers to a culture medium liquid comprising a shell and the inside of the shell.
The cell culture dish bacterial infection experimental device is characterized in that the edge of the outermost periphery of the culture medium fixing position is a first periphery, the edge of the outermost periphery of the support bottom plate is a second periphery, the first periphery is smaller than the second periphery, at least four springs are arranged between the first periphery and the second periphery at equal angular intervals, and the support bottom plate is driven by the rotation of the rotating shaft to drive the culture medium fixing position to move in each elastic direction of the springs so as to uniformly mix a culture medium and a bacterial culture solution;
the upper side of the support bottom plate is further provided with a bulge which is in the same direction as the spring, the bulge is of a T-shaped structure, the lower side of the culture medium fixing position is further provided with a groove which protrudes downwards and is in the same direction as the spring, the groove forms an inverted T shape with a hollow inner part through the support arm and is matched with the bulge, and the bulge is coupled with the groove, so that when the support bottom plate rotates, the culture medium fixing position only moves in the elastic direction of the spring and does not move in the vertical direction, and the spring does not deflect in the inelastic direction.
The cell culture dish bacterial infection experimental apparatus, temperature control module receives control module's control signal, carries out temperature control, temperature control module sets up under every culture medium, and not with the recess overlaps.
Cell culture dish bacterial infection experimental apparatus, the axis of rotation drives through first driving motor, first driving motor includes two periodic operating condition, preceding half cycle, first driving motor evenly rotates, latter half cycle, the inhomogeneous rotation of first driving motor, through inhomogeneous rotation, make the spring under the effect of different eccentric forces, control different spring productions of control, control in the elasticity direction the culture medium is fixed to be removed to the production rocks the action and makes bacterial culture solution and culture medium mixing.
The cell culture dish bacterial infection experimental device is characterized in that the uneven rotation comprises acceleration or deceleration rotation through set acceleration, change of rotation speed to form fast and slow rotation, and stop of circular rotation after rotation.
In the cell culture dish bacterial infection experimental device, the second chamber is a quantitative culture chamber, the linear culture frame is in a linear structure, the first end part of the linear structure is connected with the culture medium inlet module, and the culture medium inlet module is perpendicular to the linear culture frame; the upper side of the linear culture frame stretches across a portal frame, the portal frame is provided with a liquid feeding needle which can move along a cross bar on the upper side of the portal frame, one side of the linear culture frame is also provided with a cleaning module and a concentration adjusting module which are vertical to the linear culture frame, the cleaning module and the concentration adjusting module are both arranged on the same side of the linear culture frame as the culture medium inlet module, and the cleaning module and the concentration adjusting module are arranged right below the portal frame;
liquid feeding needle passes through the pipe connection the feed liquor module will bacterial culture solution in the feed liquor module adds earlier in the concentration adjustment module, through concentration adjustment module is according to different concentration partial shipment in different containers, through portal frame control the liquid feeding needle does not take a sample in proper order and need the bacterial culture solution of different concentrations in the container, adds the bacterial culture solution and gets into in the culture medium in the straight line culture frame, when the bacterial culture solution of different concentrations of taking a sample at every turn, deepens the liquid feeding needle and washs in the cleaning module.
Cell culture dish bacterial infection experimental apparatus, the linear culture frame sets up a plurality of positions of holding, the linear culture frame still includes linear guide, driving band, the driving band drive hold the position and follow linear guide carries out the perpendicular to the removal of portal frame upside horizontal pole to make every culture medium move and carry out the liquid feeding under the portal frame.
The cell culture dish bacterial infection experimental device is characterized in that a mark module is arranged at an inlet of the first end part, the mark module marks when a culture medium enters a containing position of the linear culture frame, a mark reading module is arranged on the side edge of a linear guide rail of the linear culture frame facing the portal frame, when the culture medium moves to the lower part of the portal frame, the mark reading module reads a first mark of the culture medium positioned right below the portal frame at the moment, the read first mark is sent to a control module, the control module controls the liquid adding needle to add a bacterial culture solution with a first concentration into a culture with the first mark after receiving the first mark, associates and stores the first concentration and the first mark, and associates and stores the culture medium with different concentrations after corresponding liquid adding according to different marks and bacterial culture solutions with different concentrations in sequence, only clear water was added to the last medium as a blank.
Cell culture dish bacterial infection experimental apparatus set up second temperature control module in the second bin, second temperature control module receives control module's temperature carries out temperature control, control module sends same temperature information control first temperature control module and second temperature control module at first and carries out same temperature control, then, continuously monitors the first temperature of first temperature control module and the second temperature of first bin, compares the difference of first temperature and second temperature, works as when the difference exceeds the temperature threshold, adjusts first temperature control module and carries out temperature adjustment to adjust the adjustment according to temperature adjustment control second temperature control module adjustment temperature.
FIG. 2 is a control block diagram of the experimental apparatus of the present invention. The liquid inlet module, the gas inlet module, the cell culture module, the gas outlet module and the disinfection module are all connected with the control module, and the control module also controls the rotating shaft, the bin gate, the first temperature control module and the second temperature control module; the control module controls each module to act, and preferably, the control module also receives signals of the first temperature control module.
FIG. 3 is a schematic diagram of the operation method of the bacterial infection experimental apparatus of the cell culture dish of the present invention. The method comprises the following steps:
initializing, and starting a disinfection module by a control module for disinfection; adding a bacteria culture solution into the bacteria culture bottle, and preventing the culture medium from entering the module;
controlling the culture medium to respectively enter the disc culture rack and the linear culture rack, selecting one culture medium entering the disc culture rack to align to the bin gate, controlling the bin gate to be opened, and conveying the selected culture medium to the second bin;
after the culture medium is conveyed, the control module controls the bacteria culture solution to enter the second chamber firstly;
the method specifically comprises the following steps: the liquid feeding needle is controlled by the portal frame to sequentially sample bacterial culture liquids with different concentrations in different containers, the bacterial culture liquids are added into the culture medium entering the linear culture frame, and when the bacterial culture liquids with different concentrations are sampled each time, the liquid feeding needle is deeply inserted into the cleaning module to be cleaned;
after the bacterial culture solution enters the second bin to complete concentration adjustment, the bacterial culture solution with different concentrations is added into a culture medium on the linear culture frame, the control module opens the atomization module to spray the atomized bacterial culture solution to the first bin, temperature control is performed, and cells are cultured;
setting culture time, carrying out cell culture comparison judgment of the first chamber and the second chamber, and recording and storing.
The beneficial technical effects obtained by the invention are as follows: the invention can carry out a plurality of experimental controls during cell culture, can operate in a full-automatic mode, is convenient and intuitive to observe experimental variables, and can conveniently and accurately obtain the experimental result of cell culture in a natural non-quantitative and accurate quantitative mode by automatic culture medium temperature regulation, liquid adding and sampling. The invention has the main improvement points that two different culture platforms are arranged aiming at two different bacterial infection modes, natural infection is simulated by a disc mode to culture cells, bacterial culture solution is fully mixed by the rotation of the disc, quantitative liquid adding is carried out by a linear mode, and the volume of an experimental device is saved. The other improvement mode of the invention is that the diameters of the support frame and the culture medium fixing position are different, the centrifugal force of the rotation of the disc is utilized to be matched with the spring, so that the culture medium fixing position moves on the support frame, bacteria in a natural state are fully absorbed when the disc rotates, and the bacteria are fully mixed under the matching of the spring. As the improvement point of the invention, the bacteria culture solution with different concentrations can be marked by the marking information, the control module can be conveniently identified, the linear guide rail is arranged, the volume of the experimental device can be effectively saved, and particularly, the entering module, the cleaning module and the like on the periphery of the linear guide rail are vertical to the linear guide rail.
The first chamber and the third chamber can be opened or isolated, and the culture medium is controlled to be transferred from the first chamber to the third chamber through the disc, so that the volume of the whole experimental equipment is reduced.
Although the invention has been described above with reference to various embodiments, it should be understood that many changes and modifications may be made without departing from the scope of the invention. That is, the methods, systems or devices discussed above are examples. Various configurations may omit, substitute, or add various procedures or components as appropriate. For example, in alternative configurations, the methods may be performed in an order different than that described, and/or various stages may be added, omitted, and/or combined. Also, features described with respect to certain configurations may be combined in various other configurations. Different aspects and elements of the configuration may be combined in a similar manner. Furthermore, many of the elements that follow as technology develops are merely examples and do not limit the scope of the disclosure or claims.
It is intended that the foregoing detailed description be regarded as illustrative rather than limiting, and that it be understood that it is the following claims, including all equivalents, that are intended to define the spirit and scope of this invention. The above examples are to be construed as merely illustrative and not limitative of the remainder of the disclosure. After reading the description of the invention, the skilled person can make various changes or modifications to the invention, and these equivalent changes and modifications also fall into the scope of the invention defined by the claims.
Claims (10)
1. The utility model provides a cell culture dish bacterial infection experimental apparatus, supports through the support, its characterized in that includes: the device comprises a liquid inlet module, a gas inlet module, a cell culture module, a gas outlet module, a disinfection module and a control module; the air inlet module is used for entering external air; the liquid inlet module is used for conveying a bacterial culture solution to the cell culture module for cell culture; the air outlet module is used for discharging waste gas; the disinfection module is used for disinfecting the cell culture module, and the control module is used for controlling the liquid inlet module, the gas inlet module, the cell culture module, the gas outlet module and the disinfection module; the cell culture module comprises a cabin body capable of being controlled in a sealing mode, the cabin body is divided into a first cabin, a second cabin and a third cabin, the first cabin is provided with a disc culture frame, the second cabin is provided with a linear culture frame, and the third cabin is provided with a blank group frame.
2. The cell culture dish bacterial infection experimental device of claim 1, wherein the first chamber is a natural exposure infection chamber, the disc culture frame comprises a plurality of culture medium fixing positions, the culture medium fixing positions are uniformly distributed along a circular ring with the same center for fixing a culture medium, a culture medium inlet module for entering the culture medium is arranged on the side surface of the disc culture base frame, and the culture medium inlet module is used for respectively conveying the culture medium to the disc culture frame and the linear culture frame of the second chamber;
the disc is cultivateed frame upside and is set up the fixed position of a plurality of culture mediums, the downside of every fixed position of the fixed position of a plurality of culture mediums all sets up first temperature control module, and first temperature control module downside sets up supporting baseplate, the supporting baseplate downside supports through the axis of rotation.
3. The cell culture dish bacterial infection experimental device of claim 2, wherein the edge of the outermost periphery of the culture medium fixing position is a first periphery, the edge of the outermost periphery of the support bottom plate is a second periphery, the first periphery is smaller than the second periphery, at least four springs are arranged between the first periphery and the second periphery at equal angular intervals, and the support bottom plate is driven by the rotation of the rotating shaft, so that the springs can drive the culture medium fixing position to move in each elastic direction of the springs to uniformly mix the culture medium and the bacterial culture solution;
the upper side of the support bottom plate is further provided with a bulge which is in the same direction as the spring, the bulge is of a T-shaped structure, the lower side of the culture medium fixing position is further provided with a groove which protrudes downwards and is in the same direction as the spring, the groove forms an inverted T shape with a hollow inner part through the support arm and is matched with the bulge, and the bulge is coupled with the groove, so that when the support bottom plate rotates, the culture medium fixing position only moves in the elastic direction of the spring and does not move in the vertical direction, and the spring does not deflect in the inelastic direction.
4. The device for testing bacterial infection of a cell culture dish according to claim 3, wherein the temperature control module receives a control signal of the control module for temperature adjustment, and the temperature control module is arranged right below each culture medium and does not overlap with the groove.
5. The device for testing bacterial infection of a cell culture dish according to claim 3, wherein the rotating shaft is driven by a first driving motor, the first driving motor comprises two periodic working states, a first half period, the first driving motor rotates uniformly, a second half period, the first driving motor rotates non-uniformly, the springs are driven by the non-uniform rotation to control different springs to generate different elastic forces under the action of different eccentric forces, and the culture medium is controlled to move fixedly in the direction of the elastic force to generate a shaking action to mix the bacterial culture solution and the culture medium uniformly.
6. The bacterial infection experimental apparatus of the cell culture dish according to claim 5, wherein the non-uniform rotation includes acceleration or deceleration rotation by a set acceleration, changing rotation speed to a slow speed, and stopping a cycle rotation after rotation.
7. The cell culture dish bacterial infection experimental device of claim 1, wherein the second chamber is a quantitative culture chamber, the linear culture rack is of a linear structure, a culture medium inlet module is connected to a first end of the linear structure, and the culture medium inlet module is arranged perpendicular to the linear culture rack; the upper side of the linear culture frame stretches across a portal frame, the portal frame is provided with a liquid feeding needle which can move along a cross bar on the upper side of the portal frame, one side of the linear culture frame is also provided with a cleaning module and a concentration adjusting module which are vertical to the linear culture frame, the cleaning module and the concentration adjusting module are both arranged on the same side of the linear culture frame as the culture medium inlet module, and the cleaning module and the concentration adjusting module are arranged right below the portal frame;
liquid feeding needle passes through the pipe connection the feed liquor module will bacterial culture solution in the feed liquor module adds earlier in the concentration adjustment module, through concentration adjustment module is according to different concentration partial shipment in different containers, through portal frame control the liquid feeding needle does not take a sample in proper order and need the bacterial culture solution of different concentrations in the container, adds the bacterial culture solution and gets into in the culture medium in the straight line culture frame, when the bacterial culture solution of different concentrations of taking a sample at every turn, deepens the liquid feeding needle and washs in the cleaning module.
8. The cell culture dish bacterial infection experimental device of claim 7, wherein the linear culture shelf is provided with a plurality of containing positions, the linear culture shelf further comprises a linear guide rail and a driving belt, and the driving belt drives the containing positions to move along the linear guide rail and perpendicular to the cross bar on the upper side of the portal frame, so that each culture medium moves below the portal frame to be added.
9. The cell culture dish bacterial infection experimental device of claim 8, wherein the first end inlet is provided with a marking module, the marking module marks the culture medium when the culture medium enters the containing position of the linear culture shelf, a marking reading module is arranged on the side of the linear guide rail of the linear culture shelf facing the portal frame, when the culture medium moves below the portal frame, the marking reading module reads a first mark of the culture medium which is positioned right below the portal frame at the moment, the read first mark is sent to a control module, the control module controls the liquid adding needle to add the bacterial culture solution with the first concentration into the culture with the first mark after receiving the first mark, and the first concentration and the first mark are associated and stored, and the liquid adding needle is sequentially associated and stored after the culture medium with different marks and the bacterial culture solution with different concentrations are added correspondingly, only clear water was added to the last medium as a blank.
10. The device for testing bacterial infection of a cell culture dish in accordance with claim 9, wherein a second temperature control module is disposed in the second chamber, the second temperature control module receives the temperature of the control module for temperature control, the control module first sends the same temperature information to control the first temperature control module and the second temperature control module for the same temperature control, then continuously monitors the first temperature of the first temperature control module and the second temperature of the first chamber, compares the difference between the first temperature and the second temperature, adjusts the first temperature control module for temperature adjustment when the difference exceeds a temperature threshold, and controls the second temperature control module for temperature adjustment according to the temperature adjustment.
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| CN113029883A (en) * | 2021-03-24 | 2021-06-25 | 江苏瑞城检测技术有限公司 | Detection equipment for air disinfection effect |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106434303A (en) * | 2016-11-18 | 2017-02-22 | 天津开发区合普工贸有限公司 | Bacterial infection experiment device for cell culture dishes |
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| CN106434303A (en) * | 2016-11-18 | 2017-02-22 | 天津开发区合普工贸有限公司 | Bacterial infection experiment device for cell culture dishes |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113029883A (en) * | 2021-03-24 | 2021-06-25 | 江苏瑞城检测技术有限公司 | Detection equipment for air disinfection effect |
| CN113029883B (en) * | 2021-03-24 | 2022-04-12 | 江苏瑞城检测技术有限公司 | Detection equipment for air disinfection effect |
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