CN212293604U

CN212293604U - Culture apparatus for cell separation test

Info

Publication number: CN212293604U
Application number: CN202022068298.3U
Authority: CN
Inventors: 张亚楠; 赵海军; 韩晓春; 王媛; 崔宁; 赵文晓; 田升; 宋晓彤; 王世军
Original assignee: Shandong University of Traditional Chinese Medicine
Current assignee: Shandong University of Traditional Chinese Medicine
Priority date: 2020-09-08
Filing date: 2020-09-08
Publication date: 2021-01-05
Anticipated expiration: 2030-09-08

Abstract

The invention belongs to the field of cell culture, and particularly relates to a culture device for a cell separation test. This device can once only place a plurality of culture dishes, and horizontal occupation space is little, when cultivateing, can realize automatically that for cell culture keeps good nutrition state, excellent in use effect can change the in-process at the culture solution simultaneously, shakes the culture dish box body, promotes the cell and carries out the adherence again, improves the growth efficiency of cell not adhering to the wall.

Description

Culture apparatus for cell separation test

Technical Field

The invention relates to the technical field of cell culture, in particular to a culture device for cell separation tests.

Background

In recent years, in addition to pharmaceuticals containing conventional synthetic chemical substances as main components, biological agents derived from biological materials produced by biotechnology have been widely used. Among biological agents, particularly attractive are cell products such as antibodies. These biological agents are very effective, but have a problem of high price. Further, since these biological agents are products derived from living organisms, variations in quality and the like in the production process may be larger than those of conventional pharmaceuticals. Therefore, there is a need for the development of a system for producing a cell product at low cost by culturing cells in large quantities at a time, and a culture system capable of improving the quality of the cell product so that the quality of the cells can be maintained while maintaining the culture environment stably.

In cell separation experiments, it is often necessary to use a cell culture apparatus, and cells can be mainly classified into suspension cells, which are mainly suspended in a culture medium, and adherent cells, which are more likely to adhere to the surface of a culture vessel during growth, according to their growth state. In order to maintain a good nutritional state of cells in the culture process, the culture medium needs to be changed at regular time, supernatant is sucked away for adherent cells, the cells are rinsed by buffer solution, and then the new culture medium is changed. When the culture medium is sucked, a pipette gun or an electric pipette is generally used for operation, and cells are easily sucked away together with the supernatant; meanwhile, in the cell rinsing process by a pipette or an electric pipette, the cells are impacted, and the cells are possibly damaged due to overlarge local pressure in the process of adding the culture medium, so that the culture device for the cell separation test is provided.

Disclosure of Invention

The culture device for the cell separation test provided by the invention solves the problems in the background technology.

In order to achieve the purpose, the invention adopts the following technical scheme:

a cell separation experiment culture device comprises a fixed shell and a fixed column, wherein a movable plate is installed at the opening at the top end of the fixed shell, a nutrient solution storage box and a waste solution storage box are respectively arranged at two sides in the fixed shell, a motor installed on the side wall of the fixed shell is arranged between the nutrient solution storage box and the waste solution storage box, an output shaft of the motor is connected with a rotating rod rotatably connected in the fixed shell, two scroll shells fixed in the fixed shell through a support are arranged outside the rotating rod, a plurality of turbine blades fixedly connected outside the rotating rod are arranged inside the scroll shells, the liquid inlet end of one scroll shell is communicated with the nutrient solution storage box through a conduit, the liquid outlet end of the other scroll shell is communicated with the waste solution storage box through a conduit, the fixed column is fixedly connected at the top end of the movable plate, a plurality of rotating rings are sleeved outside the fixed column, the joint has the culture dish box body on the support frame, and the culture dish top cap that opens there are two through-holes is installed on the top of culture dish box body, open on top one side of support frame has the spout, and sliding connection has the movable rod of cross-section for L shape in the spout, is connected with spring two between the inner wall of movable rod and spout, and the rigid coupling has the feed liquor intubate and goes out the liquid intubate on the movable rod, and the other end of feed liquor intubate is connected with the volute that is connected nutrient solution storage box, goes out the other end of liquid intubate and is connected with the volute that.

Preferably, the equal rigid coupling in bottom four corners of fly leaf has movable post, and the top four corners of set casing has all opened the spacing groove, and movable post sliding connection is at the spacing inslot, and the rigid coupling has spring one between movable post and the spacing groove, the rigid coupling has the cam on the dwang, cam and fly leaf sliding connection.

Preferably, the internally mounted of culture dish box body has the division board, forms the intercommunication chamber of U-shaped between the inner wall of division board and culture dish box body, and the feed liquor intubate with go out the liquid intubate respectively with the both ends intercommunication in intercommunication chamber, the top surface of division board parallels with the top of culture dish box body, it has the intercommunicating pore to open on the diapire of division board, is equipped with the pellicle in the intercommunicating pore.

Preferably, the cross section of the support frame is Y-shaped, and upward extending parts are arranged at the edges of the support frame.

Preferably, a plurality of annular grooves are formed in the outer side wall of the fixing column, and the rotating ring is rotatably connected in the annular grooves.

Preferably, the inside of set casing still installs controller and time-recorder, and the input of controller is connected with the power, and the output of controller is connected with the motor, controller and time-recorder electric connection.

Preferably, the equal threaded connection in top of nutrient solution storage box and abandonment liquid storage box has the top cap, and the embedding has the balancing weight on the diapire of set casing.

Compared with the prior art, the invention has the beneficial effects that:

1. the invention provides a culture device for cell separation tests, which can be used for placing a plurality of culture dishes at one time, has small transverse occupied space, can automatically realize the replacement of culture solution during culture, keeps a good nutritional state for cell culture and has good use effect.

2. The invention provides a culture device for a cell separation test, which can vibrate a culture dish box body in the culture solution replacement process, promote cells to adhere to the wall again and improve the growth efficiency of the cells which do not adhere to the wall.

Drawings

FIG. 1 is a schematic structural view of a culture apparatus for cell isolation test according to the present invention;

FIG. 2 is a schematic structural view of the interior of a culture dish box of a culture device for cell isolation tests according to the present invention;

FIG. 3 is a schematic structural diagram of a culture dish box, a culture dish top cover, a liquid inlet cannula, a liquid outlet cannula and a partition plate of the culture device for cell separation tests, which is provided by the invention;

FIG. 4 is a schematic view showing the structure inside the fixing case of the culture apparatus for cell isolation test according to the present invention.

In the figure: the device comprises a nutrient solution storage box 1, a cam 2, a waste solution storage box 3, a fixed shell 4, a first spring, a movable column 6, a movable plate 7, a fixed column 8, a rotating ring 9, a second spring, a support frame 11, a culture dish box 12, a culture dish top cover 13, a movable rod 14, a liquid inlet insertion tube 15, a liquid outlet insertion tube 16, a separation plate 17, a semipermeable membrane 18, a communication cavity 19, a motor 20, a rotating rod 21, a volute 22 and a turbine blade 23.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1 to 4, a cell separation experiment culture apparatus includes a fixed casing 4 and a fixed column 8, a movable plate 7 is installed at an opening at a top end of the fixed casing 4, a nutrient solution storage box 1 and a waste solution storage box 3 are respectively installed at two sides inside the fixed casing 4, a motor 20 installed on a side wall of the fixed casing 4 is installed between the nutrient solution storage box 1 and the waste solution storage box 3, an output shaft of the motor 20 is connected with a rotating rod 21 rotatably connected inside the fixed casing 4, two scroll casings 22 fixed inside the fixed casing 4 through a bracket are installed outside the rotating rod 21, a plurality of turbine blades 23 fixed outside the rotating rod 21 are installed inside the scroll casing 22, a liquid inlet end of one scroll casing 22 is communicated with the nutrient solution storage box 1 through a conduit, a liquid outlet end of the other scroll casing 22 is communicated with the waste solution storage box 3 through a conduit, the fixed column 8 is fixedly, the outside of fixed column 8 rotates and has cup jointed a plurality of rotating rings 9, and the outside of rotating ring 9 is fixed with a plurality of support frames 11, and the joint has culture dish box body 12 on the support frame 11, and the culture dish top cap 13 that opens and have two through-holes is installed on the top of culture dish box body 12, open on one side of the top of support frame 11 has the spout, and sliding connection has the movable rod 14 that the cross-section is L shape in the spout, is connected with spring two 10 between the inner wall of movable rod 14 and spout, and the rigid coupling has feed liquor intubate 15 and play liquid intubate 16 on the movable rod 14, and the other end of feed liquor intubate 15 is connected with the volute 22 of being connected nutrient solution storage box 1, and the other end of play liquid intubate 16.

The equal rigid coupling in bottom four corners of fly leaf 7 has movable post 6, and the top four corners of set casing 4 has all been opened there is the spacing groove, and 6 sliding connection of activity post are at the spacing inslot, and the rigid coupling has spring 5 between activity post 6 and the spacing groove, the rigid coupling has cam 2 on the dwang 21, and cam 2 and fly leaf 7 sliding connection can drive the device and shake when changing liquid for cell in the culture dish carries out the operation of adherence again.

A partition plate 17 is arranged in the culture dish box body 12, a U-shaped communicating cavity 19 is formed between the partition plate 17 and the inner wall of the culture dish box body 12, a sealing plate is arranged at the middle position in the communicating cavity 19, the culture solution enters one end of the communicating cavity 19 through a liquid inlet insertion pipe 15, then flows into a cell culture cavity at the upper part of a partition plate 17 through a semipermeable membrane 18 at one side, then flows into a communication cavity 19 inserted with a liquid outlet cannula 16 through the semipermeable membrane 18 at the other side, is discharged from the liquid outlet cannula 16, a liquid inlet cannula 15 and the liquid outlet cannula 16 are respectively communicated with two ends of the communication cavity 19, the top surface of the partition plate 17 is parallel to the top end of the culture dish box body 12, a communication hole is arranged on the bottom wall of the partition plate 17, the semipermeable membrane 18 is arranged in the communication hole, and can prevent cells from being adsorbed away along with culture solution when changing the culture solution, meanwhile, the design of the partition plate 17 and the semipermeable membrane 18 can reduce the impact of liquid flow on cells.

The cross-section of support frame 11 is Y shape, and the edge of support frame 11 all is equipped with ascending extension, and culture dish box body 12 can the joint in support frame 11.

A plurality of annular grooves are formed in the outer side wall of the fixing column 8, the rotating ring 9 is rotatably connected in the annular grooves, a plurality of supporting frames 11 can be rotated, and the culture dish box body 12 is conveniently detached from the supporting frames.

The inside of the fixed shell 4 is also provided with a DATA-7311 universal controller and a 555 timer, the input end of the controller is connected with a power supply, the output end of the controller is connected with a motor 20, and the controller is electrically connected with the timer, so that the culture solution can be replaced at regular time.

Equal threaded connection in top of nutrient solution storage box 1 and abandonment liquid storage box 3 has the top cap, and the embedding has the balancing weight on the diapire of set casing 4, and the setting of balancing weight guarantees the stability of device when vibrations.

When the cell culture box is used, a culture solution is filled in the culture dish box body 12, the culture dish box body 12 is clamped on the support frame 1, then the culture dish top cover 13 is covered, the liquid inlet insertion tube 15 and the liquid outlet insertion tube 16 are inserted on the culture dish top cover 13 and are respectively communicated with two ends of the communicating cavity 19, after cells are cultured for a period of time, the timer sends a signal to the controller, the output end of the controller controls the motor 20 to act, the output shaft of the motor 20 drives the rotating rod 21 to rotate, the rotating rod 21 drives the cam 2 and the turbine blades 23 to rotate, the rotation of one turbine blade 23 continuously drives the nutrient solution in the nutrient solution storage box 1 to be led into the culture dish box body 12, the nutrient solution in the nutrient solution storage box 1 enters the cell culture cavity in the nutrient solution storage box 1 through the permeation of the semipermeable membrane 18, the rotation of the other turbine blade 23 continuously drives the, then flow into and store in abandonment liquid storage box 3, the nutrient solution is leading-in to culture dish box body 12 in, and when the output shaft at motor 20 drove 2 rotations of cam, constantly drive fly leaf 7 vibrations on set casing 4, fly leaf 7 drives the up-and-down motion of activity post 6, drive all culture dishes vibrations, thereby drop the cell adherence, realize the adherence again after vibrations stop, improve the growth efficiency of cell not adhering to the wall, through the setting of this device, can once only place a plurality of culture dishes, horizontal occupation space is little, when cultivateing, can realize the change to the culture solution automatically, keep good nutritive state for cell culture, excellent in use effect, can change the in-process at the culture solution, vibrate the culture dish box body, promote the cell to carry out the adherence again, improve the growth efficiency of cell not adhering to the wall.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A culture device for cell separation tests comprises a fixed shell (4) and a fixed column (8), and is characterized in that a movable plate (7) is installed at the opening at the top end of the fixed shell (4), a nutrient solution storage box (1) and a waste solution storage box (3) are respectively arranged on two sides in the fixed shell (4), a motor (20) installed on the side wall of the fixed shell (4) is arranged between the nutrient solution storage box (1) and the waste solution storage box (3), an output shaft of the motor (20) is connected with a rotating rod (21) which is rotatably connected in the fixed shell (4), two volute shells (22) which are fixed in the fixed shell (4) through a support are arranged outside the rotating rod (21), a plurality of turbine blades (23) which are fixedly connected outside the rotating rod (21) are arranged inside the volute shells (22), and the liquid inlet end of one volute shell (22) is communicated with the nutrient solution storage box (1), the liquid outlet end of the other volute (22) is communicated with the waste liquid storage box (3) through a guide pipe, the top end of the movable plate (7) is fixedly connected with a fixed column (8), the outer part of the fixed column (8) is rotatably sleeved with a plurality of rotating rings (9), the outer part of each rotating ring (9) is fixedly provided with a plurality of supporting frames (11), each supporting frame (11) is connected with a culture dish box body (12) in a clamping manner, the top end of each culture dish box body (12) is provided with a culture dish top cover (13) with two through holes, one side of the top end of each supporting frame (11) is provided with a sliding chute, a movable rod (14) with an L-shaped section is slidably connected in each sliding chute, a spring II (10) is connected between each movable rod (14) and the inner wall of each sliding chute, a liquid inlet insertion pipe (15) and a liquid outlet insertion pipe (16) are fixedly connected on each movable rod (, the other end of the liquid outlet insertion pipe (16) is connected with a scroll (22) connected with the waste liquid storage box (3).

2. The culture device for cell separation test according to claim 1, wherein the movable columns (6) are fixedly connected to four corners of the bottom end of the movable plate (7), the limiting grooves are formed in four corners of the top end of the fixed shell (4), the movable columns (6) are slidably connected in the limiting grooves, the springs (5) are fixedly connected between the movable columns (6) and the limiting grooves, the cam (2) is fixedly connected to the rotating rod (21), and the cam (2) is slidably connected with the movable plate (7).

3. The culture device for the cell separation test according to claim 1, wherein a partition plate (17) is installed inside the culture dish box body (12), a U-shaped communication cavity (19) is formed between the partition plate (17) and the inner wall of the culture dish box body (12), the liquid inlet insertion tube (15) and the liquid outlet insertion tube (16) are respectively communicated with two ends of the communication cavity (19), the top surface of the partition plate (17) is parallel to the top end of the culture dish box body (12), a communication hole is formed in the bottom wall of the partition plate (17), and a semi-permeable membrane (18) is arranged in the communication hole.

4. The culture device for cell separation test according to claim 1, wherein the cross section of the support frame (11) is Y-shaped, and the edges of the support frame (11) are provided with upward extensions.

5. The culture device for cell separation experiments as claimed in claim 1, wherein the outer side wall of the fixed column (8) is provided with a plurality of annular grooves, and the rotating ring (9) is rotatably connected in the annular grooves.

6. The culture device for cell separation test according to claim 1, wherein a controller and a timer are further installed inside the fixing case (4), the input end of the controller is connected with a power supply, the output end of the controller is connected with the motor (20), and the controller is electrically connected with the timer.

7. The culture apparatus for cell separation test according to claim 1, wherein the top ends of the nutrient solution storage box (1) and the waste solution storage box (3) are both connected with a top cover by screw threads, and a balancing weight is embedded in the bottom wall of the fixing shell (4).