CN110652539A

CN110652539A - Traditional Chinese medicine bacteriostatic composition and bacteriostatic agent

Info

Publication number: CN110652539A
Application number: CN201911049382.6A
Authority: CN
Inventors: 付涛
Original assignee: Shanghai Longxi Investment Co Ltd
Current assignee: Shanghai Longxi Investment Co Ltd
Priority date: 2019-10-31
Filing date: 2019-10-31
Publication date: 2020-01-07

Abstract

The invention provides a traditional Chinese medicine bacteriostatic composition which comprises, by weight, 0.1-2 parts of bacteriostatic raw materials, 7-50 parts of Chinese herbal medicine raw materials and 0.02-0.2 part of surfactant. The bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition disclosed by the invention has a slightly sweet taste, no bitter taste or slightly bitter taste, no adverse reaction, no tooth yellowing, complete elimination of peculiar smell after use and no relapse within one week, and can be tested by using; has good antibacterial rate on oral pathogenic bacteria such as Streptococcus mutans, Staphylococcus aureus, Candida albicans, and Escherichia coli, and does not destroy oral microecological balance. In addition, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition is mild in nature and moist, can clear heat and dry dampness, purge fire and detoxify, and meanwhile can relieve gum swelling and pain, protect oral mucosa, promote formation of enamel and establish a dynamically balanced oral micro-ecological environment.

Description

Traditional Chinese medicine bacteriostatic composition and bacteriostatic agent

Technical Field

The invention belongs to the field of bacteriostatic agents, and particularly relates to a traditional Chinese medicine bacteriostatic composition and a bacteriostatic agent.

Background

The oral cavity is the entrance of the human digestive tract communicating with the outside, and more than 700 kinds of microorganisms such as bacteria, viruses and fungi exist in the oral cavity of healthy people. Most of them are nonpathogenic resident bacteria, and they play important roles in maintaining the dynamic balance of oral hygiene environment, assisting oral function, etc. Once this dynamic balance is broken, the proportion of foreign organisms in the oral cavity or several microorganisms inherent in the oral micro-ecology is significantly increased, becoming dominant bacteria at the site of colonization, leading to the occurrence of oral diseases. Wherein, the streptococcus mutans can form a biofilm, namely a dental plaque biofilm on the surface of a tooth body to cause dental caries; the staphylococcus aureus can cause local suppuration infection and has close relation with periodontal diseases; candida albicans can cause oral diseases such as thrush, oral ulcer and gingivitis; coli can cause parenteral infection and acute diarrhea.

At present, the drugs for inhibiting pathogenic bacteria in the oral cavity are mainly divided into broad-spectrum antibiotics and chemical synthetic drugs, and Chinese herbal medicines and extracts thereof. Wherein, the long-term use of broad-spectrum antibiotics and chemical synthetic drugs can destroy the micro-ecological balance of the oral cavity while eliminating pathogenic bacteria, so that the dynamic balance of the oral cavity is easily destroyed by external bacteria; the Chinese herbal medicine and the extract thereof are accepted and accepted by the consumers because of containing natural medicinal components. However, due to the complex components in the Chinese herbal medicines and the extracts thereof, adverse reactions such as oral mucosa allergy and the like may exist in the use process of different users; most of the Chinese herbal medicines generally used for oral bacteriostasis have large cold property, and the long-term use of the Chinese herbal medicines can aggravate the oral problems; meanwhile, when the content of the bacteriostatic agent prepared from the Chinese herbal medicine and the extract thereof is low, the bacteriostatic effect is poor, and when the content is high, the taste is bitter, so that the oral cavity mucosa is stimulated, and the dynamic balance of the oral cavity is damaged. Therefore, there is a need to develop a novel bacteriostatic composition and a bacteriostatic agent.

Disclosure of Invention

In order to solve the problems, the invention provides a traditional Chinese medicine bacteriostatic composition which comprises, by weight, 0.1-2 parts of bacteriostatic raw materials, 7-50 parts of Chinese herbal medicine raw materials and 0.02-0.2 part of surfactant;

the antibacterial raw material is selected from one or more of birch leaf, birch trunk, birch bark, spina date seed, common camptotheca fruit, common camptotheca bark, common camptotheca leaf, apple bark and sweet osmanthus leaf.

As a preferred technical scheme, the Chinese herbal medicine raw materials comprise, by weight, 0.3-4 parts of scutellaria baicalensis, 0.4-5 parts of radix rehmanniae recen, 0.4-5 parts of prepared rehmannia root, 0.5-3 parts of radix ophiopogonis, 0.35-3 parts of radix paeoniae alba, 0.5-2.5 parts of rhizoma anemarrhenae, 0.5-2.5 parts of cortex moutan, 0.5-3 parts of radix scrophulariae, 0.5-3 parts of gardenia, 0.85-5 parts of bamboo leaves, 1.5-7 parts of raw dragon bones, 0.3-2.5 parts of pericarpium citri reticulatae, 0.3-2.5 parts of raw liquorice and 0.1-2 parts of viola violet.

As a preferable technical scheme, the weight ratio of the antibacterial raw material, the scutellaria baicalensis, the radix rehmanniae recen and the prepared rehmannia root is 1: (2-3): (2-4): (2-4).

As a preferred technical scheme, the weight ratio of the bacteriostatic raw material, the white paeony root, the raw liquorice and the tangerine peel is 1: (1.5-3.5): (1-3): (1-3).

As a preferable technical scheme, the surfactant is selected from one or more of cetylpyridinium chloride, sodium benzoate, sodium lauryl sulfate, potassium sorbate, sodium lauroyl sarcosinate, propyl hydroxybenzoate, betaine, hydrogenated castor oil, methyl hydroxybenzoate, benzalkonium bromide and cetylpyridinium chloride.

According to the second aspect of the invention, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition comprises, by weight, 5-60 parts of the traditional Chinese medicine bacteriostatic composition, 0.03-0.5 part of a sweetening agent, 0.1-0.3 part of tea polyphenol and 100-200 parts of purified water.

As a preferable technical scheme, the sweetening agent is selected from one or more of xylitol, stevioside, mogroside, glycyrrhizin, rubusoside, sucrose, saccharin sodium, glycerol, sorbitol, mannitol, aspartame and maltose.

As a preferable technical scheme, the weight ratio of the bacteriostatic raw material to the surfactant to the sweetener is 1: (0.1-0.2): (0.2-0.3).

As a preferable technical scheme, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition further comprises 0.01-0.05 part of aromatic in parts by weight.

As a preferable technical scheme, the aromatic is selected from one or more of menthol, thymol, eugenol, sweet orange oil, spearmint oil, wintergreen oil, lemon oil, tea extract, eucalyptus leaf essence, fennel essence and clove essence.

Has the advantages that: the bacteriostatic agent is prepared from bacteriostatic raw materials, Chinese herbal medicine raw materials, a surfactant and other traditional Chinese medicine bacteriostatic compositions, and tests show that the bacteriostatic agent has slightly sweet taste, no bitter taste or slightly bitter taste, no adverse reaction, no tooth yellowing, completely eliminated peculiar smell after use and no relapse within one week; has good antibacterial rate on oral pathogenic bacteria such as Streptococcus mutans, Staphylococcus aureus, Candida albicans, and Escherichia coli, and does not destroy oral microecological balance. In addition, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition is mild in nature and moist, and also can clear heat and dry dampness, purge fire and detoxify, control and eliminate dental plaque, gingival swelling and pain, gingival bleeding and oral ulcer, relieve the gingival swelling and pain, protect oral mucosa, promote the formation of enamel and establish a dynamically-balanced oral micro-ecological environment.

Detailed Description

The technical features of the technical solutions provided by the present invention are further clearly and completely described below with reference to the specific embodiments, and the scope of protection is not limited thereto.

The words "preferred", "more preferred", and the like, in the present invention refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention. All the components in the Chinese herbal medicine raw materials are not particularly limited to the purchasing manufacturers.

In order to solve the problems, the invention provides a traditional Chinese medicine bacteriostatic composition which comprises, by weight, 0.1-2 parts of bacteriostatic raw materials, 7-50 parts of Chinese herbal medicine raw materials and 0.02-0.2 part of surfactant.

In a preferred embodiment, the traditional Chinese medicine bacteriostatic composition comprises, by weight, 0.5-1 part of bacteriostatic raw material, 16-34.5 parts of Chinese herbal medicine raw material and 0.05-0.1 part of surfactant.

In a more preferable embodiment, the traditional Chinese medicine bacteriostatic composition comprises 0.75 part of bacteriostatic raw material, 27 parts of Chinese herbal medicine raw material and 0.09 part of surfactant in parts by weight.

< raw materials for bacteriostasis >

The bacteriostatic raw material is a substance capable of inhibiting the growth of bacteria.

In a preferred embodiment, the bacteriostatic material is selected from one or more of birch leaves, birch trunks, birch barks, spina date seeds, common camptotheca fruits, common camptotheca barks, common camptotheca leaves, apple barks and sweet osmanthus leaves.

In a more preferred embodiment, the bacteriostatic material is selected from one or more of birch leaves, birch trunks, and birch barks.

In a further preferred embodiment, the bacteriostatic material is birch bark.

(Betula alba bark)

Betula alba bark is the bark of Betula verrucosa (Latin name: Betula pendula Roth.) belonging to Betulaceae. Betula verrucosa is an arbor, up to 25 m, with a white thin sheet on its bark, which is easily peeled. The obtained betulin, also called betulin, and betulin has antiviral, protein dissolution inhibiting, damaged hair luster improving, and hair growth promoting effects, and can be used in food, cosmetic, and medicine fields.

< Chinese herbal medicine materials >

In a preferred embodiment, the Chinese herbal medicine raw materials comprise, by weight, 0.3-4 parts of scutellaria baicalensis, 0.4-5 parts of radix rehmanniae recen, 0.4-5 parts of prepared rehmannia root, 0.5-3 parts of radix ophiopogonis, 0.35-3 parts of radix paeoniae alba, 0.5-2.5 parts of rhizoma anemarrhenae, 0.5-2.5 parts of cortex moutan, 0.5-3 parts of radix scrophulariae, 0.5-3 parts of gardenia, 0.85-5 parts of bamboo leaves, 1.5-7 parts of raw dragon bones, 0.3-2.5 parts of pericarpium citri reticulatae, 0.3-2.5 parts of raw liquorice and 0.1-2 parts of violet.

In a more preferred embodiment, the Chinese herbal medicine materials comprise, by weight, 1.5-2.5 parts of radix scutellariae, 1-3 parts of radix rehmanniae recen, 1-3 parts of radix rehmanniae preparata, 1-2.5 parts of radix ophiopogonis, 1-2.5 parts of radix paeoniae alba, 1-2 parts of rhizoma anemarrhenae, 1-2 parts of cortex moutan, 1-1.5 parts of radix scrophulariae, 1-2.5 parts of fructus gardeniae, 1-3 parts of bamboo leaves, 4-5 parts of fresh dragon oyster, 0.5-2 parts of pericarpium citri reticulatae, 0.5-2 parts of fresh liquorice and 0.5-1 part of violet.

In a further preferred embodiment, the Chinese herbal medicine materials comprise, by weight, 1.8 parts of scutellaria baicalensis, 2.25 parts of radix rehmanniae recen, 2.25 parts of prepared rehmannia root, 1.8 parts of radix ophiopogonis, 1.8 parts of white peony root, 1.5 parts of rhizoma anemarrhenae, 1.5 parts of cortex moutan, 1.8 parts of radix scrophulariae, 1.8 parts of gardenia, 2.25 parts of bamboo leaves, 4.5 parts of raw dragon oyster, 1.5 parts of dried orange peel, 1.5 parts of raw liquorice and 0.75 part of violet.

(Scutellaria baicalensis)

Baikal skullcap root, also known as camellia root and native gold tea root, is the root of Scutellaria baicalensis Georgi (Latin's scientific name: Scutellaria baicaiensis). The scutellaria is bitter in taste and cold in nature, has the effects of clearing heat, drying dampness, purging fire, detoxifying, stopping bleeding, preventing miscarriage and the like, and is mainly used for treating warm diseases, upper respiratory tract infection, lung heat cough, damp-heat yellow gallbladder, pneumonia, dysentery, hemoptysis, conjunctival congestion, threatened abortion, hypertension, carbuncle, furuncle and sore and other symptoms. It contains baicalein (baicalein), baicalin, wogonin, and baicalein.

(dried rehmannia root)

Rehmannia glutinosa, also known as rehmannia glutinosa, pith of rehmannia glutinosa, original rehmannia glutinosa, and dried rehmannia glutinosa, is a fresh or dried root tuber of rehmannia glutinosa (rehmannia glutinosa, Lehmanniaglutinosia, Gaetn.) Libosch. Radix rehmanniae is a heat-clearing and blood-cooling medicine, is used for treating symptoms of heat entering nutrient blood, fever and dry mouth, crimson or red tongue and the like in warm diseases, and has the effects of clearing heat, promoting fluid production, nourishing yin and nourishing blood. It contains mannitol, small amount of stigmasterol, trace amount of campesterol, and rehmanniae radix alkali, vitamin A substances, various saccharides and various amino acids.

(prepared rehmannia root)

The prepared rehmannia root, also called as prepared rehmannia root, has the effects of enriching blood, nourishing yin, benefiting essence and replenishing marrow, and contains reducing sugar, 5-hydroxymethyl furfural, nucleoside and other components.

In a preferred embodiment, the weight ratio of the bacteriostatic raw material, the scutellaria baicalensis, the radix rehmanniae recen and the prepared rehmannia root is 1: (2-3): (2-4): (2-4).

In a more preferred embodiment, the weight ratio of the bacteriostatic raw material, the scutellaria baicalensis, the dried rehamnnia root and the prepared rehmannia root is 1: 2.4: 3: 3.

the inventor finds that the bacteriostatic raw materials of the birch bark, the scutellaria baicalensis, the radix rehmanniae recen and the prepared rehmannia root are added into the Chinese medicinal bacteriostatic composition, and particularly, the weight ratio of the bacteriostatic raw materials to the scutellaria baicalensis, the radix rehmanniae recen and the prepared rehmannia root is controlled to be 1: (2-3): (2-4): (2-4), the bacteriostatic effect can be achieved. The probable reason is that appropriate amount of betulin, betulinic acid and their derivatives extracted from cortex Betulae Pendulae can inhibit the growth activity of virus or bacteria and interfere their life cycle, but excessive amount of cortex Betulae Pendulae extract has obviously reduced antibacterial effect and is liable to destroy the balance of oral microecology; the active flavonoid compounds in the scutellaria baicalensis can influence the activity of biological enzymes through strong chelation on metal ions, and the special antibacterial mechanism of the active flavonoid compounds does not easily cause drug resistance of pathogenic bacteria. The traditional Chinese medicine theory considers that oral diseases are mainly caused by wind and fire, and have the pathological bases of deficiency of vital qi, kidney essence loss and flaring-up of deficient fire, and also have local injuries formed by wind cold, pathogenic fire and heat, which are locally depressed and can not diffuse, and heat is accumulated. The scutellaria has the efficacies of clearing heat, drying dampness, purging fire and removing toxicity, and plays a role in clearing heat and inhibiting bacteria by being combined with the antibacterial raw materials in a certain proportion as monarch drug components in the formula; an appropriate amount of radix rehmanniae has the effects of clearing heat and cooling blood and assisting monarch drug to strengthen the treatment effect, but the radix scutellariae and the radix rehmanniae are cold-natured drugs, which are easy to stimulate oral mucosa and damage spleen and kidney; the prepared rehmannia root is sweet in taste, slightly warm and moist in quality, can enrich the blood, nourish yin, supplement essence and benefit marrow, is used as a ministerial medicine component of the formula together with the dried rehmannia root in a certain proportion, further enhances the bacteriostatic and heat-clearing effects, prevents cold gas from entering the body and plays an auxiliary role.

(Erdong)

The radix asparagi and radix Ophiopogonis can be used as the radix asparagi and radix Ophiopogonis. The ophiopogon root has the functions of moistening lung, nourishing stomach and clearing heart-fire in the upper and middle jiao, and is used for treating deficiency of lung-yin and stomach-yin, restlessness, insomnia and other symptoms; tian Dong has the actions of moistening lung and nourishing kidney in the upper and lower energizers, and is indicated for deficiency of lung yin and kidney yin.

Asparagus cochinchinensis

Asparagus cochinchinensis (Lour.) Merr) root tuber, which is called Asparagus cochinchinensis (Asparagus cochinchinesis) and Asparagus cochinchinensis (Lour.) Merr) of Asparagus genus of Liliaceae family. Radix asparagi is sweet and bitter in taste and cold in nature, has the effects of nourishing yin, moistening dryness, clearing lung-heat and reducing fire, and is mainly used for treating dryness-heat cough, yin deficiency, overstrain cough, fever yin impairment, internal heat, diabetes, intestinal dryness, constipation and sore throat. It contains 19 kinds of amino acids such as glucose, asparagine, serine, threonine, proline, glycine, etc., and also contains beta-sitosterol, mucilage, fructose, etc.

Radix Ophiopogonis

Ophiopogon japonicus, also called Ophiopogon japonicus and Ophiopogon japonicus, is root tuber of Ophiopogon japonicus (Latin name: Ophiopogon japonica (L.f.) KerGawl.) belonging to Liliaceae. Radix Ophiopogonis has effects of nourishing yin, moistening lung, benefiting stomach, promoting fluid production, clearing heart fire, and relieving restlessness, and can be used for treating dry cough due to lung dryness, pulmonary abscess, overstrain cough due to yin deficiency, body fluid deficiency, thirst, vexation, insomnia, laryngopharynx pain, constipation due to intestinal dryness, hematemesis, and epistaxis. It contains ophiopogonin A, B, C and D, mucoid, glucose and vitamin A.

In a preferred embodiment, the weight ratio of the asparagus cochinchinensis to the ophiopogon japonicus is 1: (0.5 to 1.5).

In a more preferred embodiment, the weight ratio of asparagus cochinchinensis to ophiopogon japonicus is 1: 1.

(white peony root)

Radix Paeoniae alba, also known as radix Paeoniae alba and radix Paeoniae alba, is dried root of Paeonia lactiflora pall. Radix Paeoniae alba is pungent and bitter in flavor, warm in nature, and slightly toxic, has effects of nourishing blood, regulating menstruation, astringing yin, arresting sweating, softening liver, relieving pain, and suppressing liver yang, and is used for treating blood deficiency, sallow complexion, menoxenia, spontaneous perspiration, night sweat, hypochondriac pain, abdominal pain, limb spasm pain, headache, giddiness, etc. It contains paeonol, paeoniflorin, benzoic acid, methyl gallate, tetragalloyl glucose, volatile oil, fatty oil, beta-sitosterol and triterpenes.

(Anemarrhena asphodeloides)

Anemarrhena asphodeloides, named as CHIMU, LIMU, YELIAO and HERBA GROUP, is dried rhizome of Anemarrhena asphodeloides Bunge of Liliaceae, a monocotyledonous plant. Rhizoma anemarrhenae is cold and moist in nature, has intestine smoothing effect, and can be used for treating fever polydipsia, lung heat dry cough, bone steaming hectic fever, internal heat diabetes, and constipation due to intestinal dryness. It contains timosaponin, flavonoid glycoside, mucoid, saccharide, nicotinic acid, fatty oil and aromatic substance.

(root-bark of peony)

Cortex moutan, also known as moutan bark, peony root bark, moutan bark and Dangen root, is the dried root bark of Paeoniasuffrutinosa Andr, a plant of Ranunculaceae, and has the effects of clearing heat and cooling blood, clearing and reducing deficiency heat, promoting blood circulation and removing blood stasis. It contains paeonol, paeonol glycoside, paeoniflorin, hydroxy paeoniflorin, benzyl paeoniflorin, gallic acid, etc.

(radix scrophulariae)

Radix scrophulariae, also known as radix scrophulariae, Zhejiang radix scrophulariae, black ginseng and Wuyuanshen, is the root of the perennial herb radix scrophulariae (Latin's name: Scrophularia ningpoensis Hemsl.) of Scrophulariaceae. Yuanshen is bitter and salty in taste, slightly cold in nature and nontoxic, and is mainly used for treating diseases such as fever due to warm-heat, fever, polydipsia, crimson tongue, macula, bone-steaming fatigue cough, dysphoria, body fluid deficiency constipation, dryness and dim eyes, sore throat, scrofula, subcutaneous nodule, carbuncle, sore and other toxic diseases. It contains alkaloid, saccharide, sterol, amino acid, fatty acid, trace volatile oil, carotene, etc.

(Gardenia)

Gardenia jasminoides Ellis, as distinguished from Gardenia jasminoides Ellis, is the fruit of Gardenia jasminoides Ellis, a plant of Rubiaceae. Gardenia with cold nature has effects of protecting liver, promoting bile flow, lowering blood pressure, tranquilizing, stopping bleeding, and relieving swelling, and contains geniposide, crocin, and gardenin.

(bamboo leaves)

The bamboo leaf is also called Lophatherum gracile, and is a leaf of the Gramineae plant Lophatherum gracile (Lodd. exLindl.) Munro var. hennos (Mitf.) Stapf et Rendle). The herba Lophatheri has effects of clearing heat, relieving restlessness, promoting fluid production, and inducing diuresis, and can be used for treating fever polydipsia, infantile convulsion, cough with dyspnea, hematemesis, scanty and brownish urine, aphtha, and tongue sore etc., and contains phenolic components, amino acids, organic acids, saccharides, etc.

(Dragon Concha Ostreae)

The Concha Ostreae is Os Draconis and Concha Ostreae, and can be used for treating palpitation, insomnia, giddiness and tinnitus.

Raw keel

Raw keel, OsDraconis, namely WUHUA fossil, Qinghua fossil, Pink fossil and Tu fossil. The crude Os Draconis is used for treating epilepsy, mania, severe palpitation, amnesia, insomnia, dreaminess, spontaneous perspiration, night sweat, nocturnal emission, hematemesis, hematochezia, metrorrhagia, leukorrhagia, dysentery, rectocele, and ulcer. It mainly contains calcium carbonate, calcium phosphate, and small amount of Fe, Mg, Al, K, Na and Cl elements.

Raw oyster

Concha Ostreae, the Latin school name of OSTREAGIGASThunberg, is salty in cold and beneficial to yin, has the combining action of astringing, and is mainly used for treating palpitation and insomnia, vertigo and tinnitus, scrofula and subcutaneous nodule, abdominal mass, spontaneous perspiration and night sweat, seminal emission and metrorrhagia belt, stomachache and pantothenic acid, etc.

In a preferred embodiment, the weight ratio of the raw keel and the raw oyster is 1: (0.5 to 1.5).

In a more preferred embodiment, the weight ratio of the raw keel and the raw oyster is 1: 1.

(dried orange peel)

Pericarpium citri reticulatae, another name: orange peel, noble-aged, yellow orange peel, red peel, orange peel, and mandarin orange peel, which are dry mature peels of Rutaceae plant orange (Citrus reticulata Blanco) and its cultivar, and can be used for treating abdominal distention, anorexia, vomiting, diarrhea, cough, excessive phlegm, etc. It contains volatile oil such as d-limonene, hesperidin, naringenin, nobiletin, inositol, and vitamin B, C.

(raw licorice root)

Raw licorice, which is a dried product of root and rhizome of glycyrrhiza uralensis (Glycyrrhiza uralensis Fisch.) belonging to genus Glycyrrhiza of family Leguminosae. The traditional Chinese medicine composition has the effects of tonifying qi and strengthening middle warmer, relieving spasm and pain, moistening lung and arresting cough, purging fire and removing toxicity, and is mainly used for treating symptoms such as listlessness and poor appetite, emaciation and sallow complexion, palpitation and shortness of breath, abdominal pain and loose stool, limb spasm and pain, hysteria, cough and asthma, sore throat, carbuncle sore and gall, infantile fetal toxicity, drug poisoning, food poisoning and the like. It contains liquiritin, liquiritigenin, isoliquiritin, isoliquiritigenin, neoliquiritin, etc.

In a preferred embodiment, the weight ratio of the bacteriostatic raw material, the white paeony root, the raw liquorice and the tangerine peel is 1: (1.5-3.5): (1-3): (1-3).

In a more preferred embodiment, the weight ratio of the bacteriostatic raw material, the white paeony root, the raw liquorice and the tangerine peel is 1: 2.4: 2: 2.

repeated researches by the inventor show that the white paeony root, the raw liquorice and the dried orange peel are added into the system, and particularly the weight ratio of the bacteriostatic raw materials to the white paeony root to the raw liquorice to the dried orange peel is 1: (1.5-4.5): (1-3): (1-3), the bacteriostatic effect of the composition is further improved. The inventor believes that the specific volatile substances in the white peony root and the flavonoid substances in the liquorice and the dried orange peel can inhibit the growth of different bacteria in the oral cavity, thereby playing a role in inhibiting bacteria; from the perspective of traditional Chinese medicine, the white peony root is warm in nature and has an analgesic effect, while the dried orange peel is warm in nature, regulates qi and strengthens spleen, eliminates dampness and reduces phlegm, and the dried orange peel are used as adjuvant drug ingredients of the formula to assist monarch and minister drugs in enhancing the treatment effect and conditioning oral health, and simultaneously further balance drug properties and prevent the spleen and kidney from being damaged by cold; a certain amount of raw liquorice can tonify qi and strengthen middle warmer, and can moderate cold property of other formulas as a guiding drug component of the formula, thereby playing a role in harmonizing drug properties.

(Zidi Ding)

Viola yedoensis Makino, Viola liaotungensis Rehd, rice sack, multiple flower rice sack, Astragalus sinicus, furunculosis, and Viola reticulata are root-carrying herbs of Viola yedoensis Makino (Viola philippica) belonging to Viola of Violaceae. It has effects in clearing away heat and toxic materials, and treating snake and insect bite, and contains glycosides, flavonoids, and wax (esters such as cerotic acid and unsaturated acid).

< surfactant >

In a preferred embodiment, the surfactant is selected from one or more of cetylpyridinium chloride, sodium benzoate, sodium lauryl sulfate, potassium sorbate, sodium lauroyl sarcosinate, propyl hydroxybenzoate, betaine, hydrogenated castor oil, methyl hydroxybenzoate, benzalkonium bromide, and cetylpyridinium chloride.

In a more preferred embodiment, the surfactant is selected from one or more of cetylpyridinium chloride, sodium benzoate, potassium sorbate, propylparaben, methylparaben, benzalkonium bromide, and cetylpyridinium chloride.

In a more preferred embodiment, the surfactant is cetylpyridinium chloride.

(xipyrazamine)

Cetylpyridinium chloride, CAS number 123-03-5, is a white to off-white crystalline or crystalline powder that is a nitrogen-containing cationic surfactant.

The cetylpyridinium chloride was purchased from carbofuran technologies ltd.

In a preferred embodiment, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition comprises, by weight, 7-53 parts of the traditional Chinese medicine bacteriostatic composition, 0.1-0.3 part of a sweetening agent, 0.15-0.25 part of tea polyphenol and 140-170 parts of purified water.

In a more preferable embodiment, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition comprises, by weight, 27.84 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol and 157 parts of purified water.

< purified Water >

The purified water is water for medicine prepared by distilling drinking water, ion exchange method, reverse osmosis method or other suitable method, and does not contain any additive.

The method for preparing the purified water is not particularly limited in the present invention, and a preparation method well known to those skilled in the art may be used.

< tea polyphenols >

Tea polyphenols, also known as "Yioxiling", Wei polyphenol and Fang Ha Ling ", are the general names of polyphenols in tea.

In a preferred embodiment, the tea polyphenol is selected from one or more combinations of EC catechin, ECG catechin, EGC catechin, EGCG catechin.

In a more preferred embodiment, the tea polyphenol is EGCG catechin.

The EGCG catechin, epigallocatechin gallate (EGCG), CAS number 989-51-5, was purchased from Nanjing spring and autumn bioengineering Co., Ltd.

< sweetening agent >

The sweetener is a food additive capable of masking salty taste, astringent taste and bitter taste of the medicine.

In a preferred embodiment, the sweetener is selected from one or more of xylitol, stevioside, mogroside, glycyrrhizin, rubusoside, sucrose, sodium saccharin, glycerin, sorbitol, mannitol, aspartame, and maltose.

In a more preferred embodiment, the sweetener is xylitol.

Xylitol, its preparation method and use

Xylitol is a natural sweetener extracted from plant materials such as birch, oak, corn cob, bagasse, etc.

The xylitol is purchased from Shandong Futian pharmaceutical Co.

In a preferred embodiment, the weight ratio of the bacteriostatic raw material, the surfactant and the sweetener is 1: (0.1-0.2): (0.2-0.3).

In a more preferred embodiment, the weight ratio of the bacteriostatic raw material, the surfactant and the sweetener is 1: 0.12: 0.25.

the inventor finds that when the surfactant, especially the cetylpyridinium chloride is added, the bacteriostatic effect of the composition can be further improved, and the probability of adverse reaction of a user is reduced. The inventor believes that the hydrophilic and lipophilic groups characteristic of cetylpyridinium chloride have good permeability when in contact with bacteria, fungi, viruses and the like, so that the bacteria, fungi, viruses and the like are inactivated; a small amount of cetylpyridinium chloride is combined with the bacteriostatic raw materials and the Chinese herbal medicine raw materials in the system, so that the bacteriostatic effect of the composition is further improved under the two aspects of inhibiting the reproduction of bacteria and inactivating the original bacteria. Because the Chinese herbal medicines have complex components, adverse reactions such as oral mucosa allergy and the like may exist in the using process of different users, and the special groups on the cetylpyridinium chloride also enable the cetylpyridinium chloride to have certain biological adhesion, so that a protective film can be formed on the oral mucosa, and the adhesion of pathogenic bacteria and the occurrence of adverse reactions in use are reduced. However, excessive cetylpyridinium chloride can destroy the microecological balance of the oral cavity and can stain demineralized teeth while removing pathogenic bacteria.

The inventor unexpectedly finds that when the sweetener xylitol is added into the system, particularly the weight ratio of the added bacteriostatic raw material, the surfactant and the sweetener is 1: (0.1-0.2): (0.2-0.3), the bad taste of the product can be improved, the antibacterial effect is obviously improved, and the phenomenon that the surfactant causes tooth coloring can be avoided to a certain extent. The inventor believes that pathogenic bacteria in the oral cavity can adhere to a biofilm formed on the surface of teeth by residual carbohydrate such as cane sugar and salivary protein after eating, acid products are generated by metabolic fermentation of the sugar-containing substances, acid products invade from weak structures of hard tissues of the teeth, and the acid products are dissolved to destroy tooth enamel to cause demineralization, so that the self health of the oral cavity is further destroyed; xylitol can not be fermented by pathogenic bacteria, can inhibit the growth and metabolism of pathogenic bacteria and the like, change the adhesion performance of the xylitol, promote tooth remineralization, inhibit demineralization and contribute to the formation of healthy enamel.

In a preferred embodiment, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition further comprises 0.01-0.05 part of an aromatic in parts by weight.

In a more preferable embodiment, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition further comprises 0.02-0.04 part by weight of an aromatic.

In a further preferred embodiment, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition further comprises 0.037 part of an aromatic in parts by weight.

< fragrance agent >

Fragrances, are fragrances and perfumes which are used in pharmaceutical preparations to improve the odor of the preparations.

In a preferred embodiment, the flavoring agent is selected from one or more of menthol, thymol, eugenol, sweet orange oil, spearmint oil, wintergreen oil, lemon oil, tea extract, eucalyptus essence, anise essence, clove essence.

In a more preferred embodiment, the fragrance is menthol.

Menthol crystal

Menthol, CAS No. 89-78-1, is a chemical agent extracted from the leaves and stems of peppermint.

The menthol is purchased from Jiangsu Caosheng Biotech limited.

The third aspect of the present invention provides an application of the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, and the bacteriostatic agent is applied to toothpaste, mouthwash, oral spray, oral bacteriostatic powder, oral care bacteriostatic liquid, oral bacteriostatic cream, oral bacteriostatic liquid, oral cleanser, oral bacteriostatic tablet, and oral cleaning towel, but is not limited thereto.

The fourth aspect of the invention provides a preparation method of a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises the following steps:

the method comprises the following steps: adding 50-60 parts of ethanol into 100 parts of purified water, adding the cleaned and granulated antibacterial raw materials into the purified water, heating and refluxing for 30-50 min under ultrasonic treatment, and soaking for 8-10 h; carrying out reduced pressure concentration treatment on the filtered extracting solution to obtain solid powder;

step two: cleaning the Chinese herbal medicine raw materials, soaking in purified water for 1-2 h, cooking for 4-6 h, and filtering; concentrating the obtained filtrate, extracting for 50-70 h by using 75% ethanol by volume fraction, centrifuging, and concentrating under reduced pressure to obtain an extract;

step three: and (3) adding the solid powder obtained in the step one, the extract obtained in the step two, a surfactant, a sweetening agent, tea polyphenol and an aromatic into purified water, stirring for 30-50 min, sterilizing and filling to obtain the bacteriostatic agent.

In a preferred embodiment, the preparation method of the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition comprises the following steps:

the method comprises the following steps: adding 55 parts of ethanol into 100 parts of purified water, adding the cleaned and granulated antibacterial raw materials into the purified water, heating and refluxing for 40min under ultrasonic treatment, and soaking for 9 h; carrying out reduced pressure concentration treatment on the filtered extracting solution to obtain solid powder;

step two: cleaning the Chinese herbal medicine materials, soaking in purified water for 1.5h, decocting for 5h, and filtering; concentrating the obtained filtrate, extracting with 75% ethanol for 60h, centrifuging, and concentrating under reduced pressure to obtain extract;

step three: and (3) adding the solid powder obtained in the step one, the extract obtained in the step two, a surfactant, a sweetening agent, tea polyphenol and an aromatic into purified water, stirring for 40min, sterilizing and filling to obtain the bacteriostatic agent.

The present invention will now be described in detail by way of examples, and the starting materials used are commercially available unless otherwise specified.

Examples

Example 1

Embodiment 1 provides a traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 0.75 parts of bacteriostatic raw materials, 27 parts of Chinese herbal medicine raw materials and 0.09 parts of surfactant.

The bacteriostatic raw material is birch bark.

The Chinese herbal medicine raw materials comprise 1.8 parts of scutellaria baicalensis, 2.25 parts of radix rehmanniae recen, 2.25 parts of prepared rehmannia root, 1.8 parts of radix ophiopogonis, 1.8 parts of white peony root, 1.5 parts of rhizoma anemarrhenae, 1.5 parts of cortex moutan, 1.8 parts of radix scrophulariae, 1.8 parts of gardenia, 2.25 parts of bamboo leaves, 4.5 parts of raw dragon oyster, 1.5 parts of dried orange peel, 1.5 parts of raw liquorice and 0.75 part of violet.

The Erdong is radix asparagi and radix Ophiopogonis; the weight ratio of the radix asparagi to the radix ophiopogonis is 1: 1; the raw dragon oyster is raw dragon bone and raw oyster; the weight ratio of the raw keel to the raw oyster is 1: 1.

the surfactant is cetylpyridinium chloride.

Embodiment 1 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 27.84 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

The sweetener is xylitol; the tea polyphenol is EGCG catechin; the aromatic is menthol.

The preparation method of the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition comprises the following steps:

Example 2

Embodiment 2 provides a traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 0.1 part of bacteriostatic raw material, 7 parts of Chinese herbal medicine raw material and 0.02 part of surfactant.

The bacteriostatic raw material is birch bark.

The Chinese herbal medicine raw materials comprise 0.3 part of radix scutellariae, 0.4 part of radix rehmanniae recen, 0.4 part of radix rehmanniae preparata, 0.5 part of radix ophiopogonis, 0.35 part of radix paeoniae alba, 0.5 part of rhizoma anemarrhenae, 0.5 part of cortex moutan, 0.5 part of radix scrophulariae, 0.5 part of fructus gardeniae, 0.85 part of bamboo leaves, 1.5 parts of raw dragon oyster, 0.3 part of dried orange peel, 0.3 part of raw liquorice and 0.1 part of violet.

the surfactant is cetylpyridinium chloride.

Embodiment 2 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises 7.12 parts of the traditional Chinese medicine bacteriostatic composition, 0.03 part of a sweetening agent, 0.1 part of tea polyphenol, 100 parts of purified water and 0.01 part of a flavoring agent in parts by weight.

The preparation method of the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition is the same as that in example 1.

Example 3

Embodiment 3 provides a traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 2 parts of bacteriostatic raw materials, 50 parts of Chinese herbal medicine raw materials and 0.2 part of surfactant.

The bacteriostatic raw material is birch bark.

The Chinese herbal medicine raw materials comprise 4 parts of scutellaria baicalensis, 5 parts of radix rehmanniae recen, 5 parts of prepared rehmannia root, 3 parts of radix ophiopogonis, 3 parts of white paeony root, 2.5 parts of rhizoma anemarrhenae, 2.5 parts of cortex moutan, 3 parts of radix scrophulariae, 3 parts of gardenia, 5 parts of bamboo leaves, 7 parts of raw dragon oyster, 2.5 parts of dried orange peel, 2.5 parts of raw liquorice and 2 parts of violet.

the surfactant is cetylpyridinium chloride.

Embodiment 3 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 52.2 parts of the traditional Chinese medicine bacteriostatic composition, 0.5 part of a sweetening agent, 0.3 part of tea polyphenol, 200 parts of purified water and 0.05 part of a flavoring agent.

Example 4

Embodiment 4 provides a traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 0.5 part of a bacteriostatic raw material, 16 parts of a Chinese herbal medicine raw material and 0.05 part of a surfactant.

The bacteriostatic raw material is birch bark.

The Chinese herbal medicine raw materials comprise 1.5 parts of scutellaria baicalensis, 1 part of radix rehmanniae recen, 1 part of prepared rehmannia root, 1 part of radix ophiopogonis, 1 part of radix paeoniae alba, 1 part of rhizoma anemarrhenae, 1 part of cortex moutan, 1 part of radix scrophulariae, 1 part of cape jasmine fruit, 1 part of bamboo leaf, 4 parts of raw dragon oyster, 0.5 part of pericarpium citri reticulatae, 0.5 part of raw liquorice and 0.5 part of violet.

the surfactant is cetylpyridinium chloride.

Embodiment 4 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises 16.55 parts of the traditional Chinese medicine bacteriostatic composition, 0.1 part of a sweetening agent, 0.15 part of tea polyphenol, 140 parts of purified water and 0.02 part of a flavoring agent in parts by weight.

Example 5

Embodiment 5 provides a traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 1 part of bacteriostatic raw material, 34.5 parts of Chinese herbal medicine raw material and 0.1 part of surfactant.

The bacteriostatic raw material is birch bark.

The Chinese herbal medicine raw materials comprise 2.5 parts of scutellaria baicalensis, 3 parts of radix rehmanniae recen, 3 parts of prepared rehmannia root, 2.5 parts of radix ophiopogonis, 2.5 parts of white paeony root, 2 parts of rhizoma anemarrhenae, 2 parts of cortex moutan, 1.5 parts of radix scrophulariae, 2.5 parts of gardenia, 3 parts of bamboo leaves, 5 parts of raw dragon oyster, 2 parts of dried orange peel, 2 parts of raw liquorice and 1 part of violet.

the surfactant is cetylpyridinium chloride.

Embodiment 5 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 35.6 parts of the traditional Chinese medicine bacteriostatic composition, 0.3 part of a sweetening agent, 0.25 part of tea polyphenol, 170 parts of purified water and 0.04 part of a flavoring agent.

Example 6

Embodiment 6 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, and is different in that 1.8 parts by weight of scutellaria baicalensis in the Chinese herbal medicine raw materials are replaced by 1.35 parts by weight.

Embodiment 6 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 27.39 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 7

Embodiment 7 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, except that 1.8 parts by weight of scutellaria baicalensis in the Chinese herbal medicine raw materials are replaced by 2.4 parts by weight.

Embodiment 7 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 28.44 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 8

Embodiment 8 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, except that the weight parts of radix rehmanniae recen in the Chinese herbal medicine raw materials are replaced by 1.35 parts from 2.25 parts.

Embodiment 8 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 26.94 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 part of a sweetening agent, 0.2 part of tea polyphenol, 157 parts of purified water and 0.037 part of a flavoring agent.

Example 9

Embodiment 9 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation, except that 2.25 parts by weight of radix rehmanniae recen is replaced by 3.15 parts by weight of the Chinese herbal medicine raw materials.

Embodiment 9 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 28.74 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 10

Embodiment 10 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, and is different in that 2.25 parts by weight of cooked rehmannia root in the Chinese herbal medicine raw materials are replaced by 1.35 parts by weight.

Embodiment 10 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 26.94 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 11

Embodiment 11 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation, and is different in that 2.25 parts by weight of cooked rehmannia root in the Chinese herbal medicine raw materials are replaced by 3.15 parts by weight.

Embodiment 11 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 28.74 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 12

Embodiment 12 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, except that the traditional Chinese medicine raw materials comprise 0.975 parts of radix paeoniae alba instead of 1.8 parts of radix paeoniae alba.

Embodiment 12 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 27.015 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 13

Embodiment 13 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, except that the traditional Chinese medicine raw materials comprise, by weight, 1.8 parts of radix paeoniae alba and 2.85 parts of radix paeoniae alba.

Embodiment 13 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises 28.89 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 part of a sweetening agent, 0.2 part of tea polyphenol, 157 parts of purified water and 0.037 part of a flavoring agent in parts by weight.

Example 14

Example 14 provides a traditional Chinese medicine bacteriostatic composition, which is similar to example 1 in specific implementation manner, except that the weight parts of raw liquorice in the Chinese herbal medicine raw materials are replaced by 0.6 part from 1.5 parts.

Embodiment 14 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 26.94 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 15

Embodiment 15 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, except that 1.5 parts by weight of raw liquorice in the Chinese herbal medicine raw materials are replaced by 2.4 parts by weight.

Embodiment 15 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 28.74 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 16

Embodiment 16 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, and is different in that 1.5 parts by weight of pericarpium citri reticulatae in the Chinese herbal medicine raw materials are replaced by 0.6 part by weight of pericarpium citri reticulatae.

Embodiment 16 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 26.94 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 17

Embodiment 17 provides a traditional Chinese medicine bacteriostatic composition, which is similar to embodiment 1 in specific implementation manner, and is different in that 1.5 parts by weight of pericarpium citri reticulatae in the Chinese herbal medicine raw materials are replaced by 2.4 parts by weight.

Embodiment 17 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 28.74 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 18

Example 18 provides a traditional Chinese medicine bacteriostatic composition, which is similar to example 1 in specific implementation, except that the surfactant xipyradine is replaced by 0.06 parts by weight from 0.09 parts.

Embodiment 18 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 27.81 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 19

Example 19 provides a traditional Chinese medicine bacteriostatic composition, which is similar to example 1 in specific implementation, except that the surfactant xipyradine is replaced by 0.09 parts by weight to 0.165 parts by weight.

Embodiment 19 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises 27.915 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 part of a sweetening agent, 0.2 part of tea polyphenol, 157 parts of purified water and 0.037 part of a flavoring agent in parts by weight.

Example 20

Example 20 provides a traditional Chinese medicine bacteriostatic composition, and the specific implementation manner is the same as example 1.

Embodiment 20 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 27.84 parts of the traditional Chinese medicine bacteriostatic composition, 0.135 parts of a sweetening agent, 0.2 parts of tea polyphenol, 157 parts of purified water and 0.037 parts of a flavoring agent.

Example 21

Example 21 provides a traditional Chinese medicine bacteriostatic composition, and the specific implementation manner is the same as example 1.

Embodiment 21 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 27.84 parts of the traditional Chinese medicine bacteriostatic composition, 0.24 part of a sweetening agent, 0.2 part of tea polyphenol, 157 parts of purified water and 0.037 part of a flavoring agent.

Example 22

Embodiment 22 provides a traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 0.75 parts of bacteriostatic raw materials, 27 parts of Chinese herbal medicine raw materials and 0.09 parts of surfactant.

The bacteriostatic raw material is birch bark.

the surfactant is cetylpyridinium chloride.

Embodiment 22 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 27.84 parts of the traditional Chinese medicine bacteriostatic composition, 0.185 parts of a sweetening agent, 0.2 parts of tea polyphenol and 157 parts of purified water.

The sweetener is xylitol; the tea polyphenol is EGCG catechin.

step three: and (3) adding the solid powder obtained in the step one, the extract obtained in the step two, a surfactant, a sweetening agent and tea polyphenol into purified water, stirring for 40min, sterilizing and filling to obtain the bacteriostatic agent.

Example 23

Embodiment 23 provides a traditional Chinese medicine bacteriostatic composition, which comprises, by weight, 0.75 parts of bacteriostatic raw materials, 27 parts of Chinese herbal medicine raw materials and 0.09 parts of surfactant.

The bacteriostatic raw material is birch bark.

the surfactant is cetylpyridinium chloride.

Embodiment 23 also provides a bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition, which comprises 27.84 parts of the traditional Chinese medicine bacteriostatic composition.

step three: mixing the solid powder obtained in the step one, the extract obtained in the step two and a surfactant, stirring for 40min, sterilizing and filling to obtain the bacteriostatic agent.

Evaluation of Performance

1. The use test: the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition obtained in the embodiment 1-21 is prepared into mouthwash according to a method well known by the person skilled in the art. 105 volunteers were randomly selected, and 5 volunteers were divided into 21 groups. The volunteers rinsed with clear water for 3 times, and after rinsing with 20mL of physiological saline for 1min, the mouth rinse prepared in examples 1-21 was used for 1min, and after using for 7 days according to the above method, the mouth rinse was evaluated for use. The taste evaluation criteria were that only 3 points were scored for sweetness, 2 points were scored for slight sweetness, no bitterness or slight bitterness, 1 point was scored for moderate bitterness, 0 point was scored for strong bitterness, and after unifying the ideas, the scores were scored in table 1; standard of oral malodor: the average score was shown in table 1, where the odor was completely removed and 3 points were recorded for no recurrence in one week, 2 points were recorded for odor removal but 2 points were recorded for recurrence in one week, 1 point was recorded for odor reduction, and 0 point was recorded for odor non-remission. The standard of tooth yellowing is that teeth in 5 volunteers are not yellow for 1 point, but teeth are yellow for 0 point if people appear; the evaluation criteria for adverse reactions were that 1 point was assigned to 5 volunteers who did not have any adverse reaction, but 0 point was assigned to any person who had an adverse reaction (e.g., oral ulcer, swollen and red gum, swollen gum, and impaired taste), and the results are shown in Table 1.

2. And (3) testing the bacteriostatic activity:

bacterial suspension culture: BHI liquid culture medium, BP culture medium, Sabouraud's culture medium and LB culture medium are respectively used for culturing streptococcus mutans, staphylococcus aureus, candida albicans and escherichia coli. The lawn cultured by four culture media is cleaned by 0.03mol/L phosphate buffer solution containing 2% peptone by mass part to obtain the lawn with the bacterial content of 1 multiplied by 10⁸CFU/mL of bacterial suspension.

② bacteriostasis test: and (3) taking a filter paper sheet, beating the filter paper sheet into a circular paper sheet by using a 0.6cm puncher, respectively dropwise adding 5 mu L of bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition obtained in the embodiment 1-23 onto the paper sheet by using a liquid transfer gun, and fully absorbing to prepare a sample sheet. Pipette 100. mu.L of the bacterial suspension onto TSC agar medium and spread evenly 3 times with a spreader. Gently sticking the sample on the culture plate with sterile forceps, sticking 1 sample on each plate, culturing in constant temperature incubator at 37 deg.C for 24 hr with normal saline as control, calculating antibacterial rate, repeating the test for 3 times, and taking average value.

Table 1 results of performance testing

The bacteriostatic agents prepared from the traditional Chinese medicine bacteriostatic composition obtained in the embodiments 1-5 all meet the requirements of sensory indexes, physical and chemical indexes, health indexes and the like in QB/T2945-.

The combination of the above experimental results shows that: according to the bacteriostatic agent prepared from the bacteriostatic composition of traditional Chinese medicines such as bacteriostatic raw materials, Chinese herbal medicine raw materials and surfactants, the use test shows that the bacteriostatic agent tastes slightly sweet, has no bitter taste or slightly bitter taste, has no adverse reaction, can not cause tooth yellowing, can completely eliminate peculiar smell after use, and can not relapse within one week; in the bacteriostasis test, the bacteriostasis rate to streptococcus mutans, staphylococcus aureus, candida albicans and escherichia coli is more than 95%, and the bacteriostasis effect is excellent. In addition, the bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition is mild in nature and moist, can clear heat and dry dampness, purge fire and detoxify, and meanwhile can relieve gum swelling and pain, protect oral mucosa, promote formation of enamel and establish a dynamically balanced oral micro-ecological environment.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. The invention is not limited to the embodiments described above, but rather, many modifications and variations may be made by one skilled in the art without departing from the scope of the invention.

Claims

1. A traditional Chinese medicine bacteriostatic composition is characterized by comprising 0.1-2 parts by weight of bacteriostatic raw materials, 7-50 parts by weight of Chinese herbal medicine raw materials and 0.02-0.2 part by weight of surfactant;

2. The bacteriostatic composition according to claim 1, wherein the Chinese herbal materials comprise, by weight, 0.3-4 parts of scutellaria baicalensis, 0.4-5 parts of radix rehmanniae recen, 0.4-5 parts of prepared rehmannia root, 0.5-3 parts of radix ophiopogonis, 0.35-3 parts of radix paeoniae alba, 0.5-2.5 parts of rhizoma anemarrhenae, 0.5-2.5 parts of cortex moutan, 0.5-3 parts of radix scrophulariae, 0.5-3 parts of gardenia, 0.85-5 parts of bamboo leaves, 1.5-7 parts of immature dragon oyster, 0.3-2.5 parts of pericarpium citri reticulatae, 0.3-2.5 parts of raw licorice and 0.1-2 parts of violet.

3. The traditional Chinese medicine bacteriostatic composition according to claim 2, wherein the weight ratio of the bacteriostatic raw material to the scutellaria baicalensis, the radix rehmanniae recen and the prepared rehmannia root is 1: (2-3): (2-4): (2-4).

4. The traditional Chinese medicine bacteriostatic composition according to claim 2, wherein the weight ratio of the bacteriostatic raw material to the white paeony root to the raw liquorice to the dried orange peel is 1: (1.5-3.5): (1-3): (1-3).

5. The traditional Chinese medicine bacteriostatic composition according to claim 1, wherein the surfactant is selected from one or more of cetylpyridinium chloride, sodium benzoate, sodium lauryl sulfate, potassium sorbate, sodium lauroyl sarcosinate, propyl hydroxybenzoate, betaine, hydrogenated castor oil, methyl hydroxybenzoate, benzalkonium bromide and cetylpyridinium chloride.

6. A bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition according to any one of claims 1-5, which is characterized by comprising, by weight, 5-60 parts of the traditional Chinese medicine bacteriostatic composition, 0.03-0.5 part of a sweetening agent, 0.1-0.3 part of tea polyphenol and 100-200 parts of purified water.

7. The bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition according to claim 6, wherein the sweetening agent is one or more selected from xylitol, stevioside, mogroside, glycyrrhizin, rubusoside, sucrose, saccharin sodium, glycerol, sorbitol, mannitol, aspartame and maltose.

8. The bacteriostatic agent prepared from the Chinese medicinal bacteriostatic composition according to claim 6 or 7, wherein the weight ratio of the bacteriostatic raw material to the surfactant to the sweetening agent is 1: (0.1-0.2): (0.2-0.3).

9. The bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition according to claim 6 or 7, which is characterized by further comprising 0.01-0.05 part of an aromatic in parts by weight.

10. The bacteriostatic agent prepared from the traditional Chinese medicine bacteriostatic composition according to claim 9, wherein the aromatic is selected from one or more of menthol, thymol, eugenol, sweet orange oil, spearmint oil, wintergreen oil, lemon oil, tea extract, eucalyptus essence, fennel essence and clove essence.