CN110627562A - High-quality amino acid water-soluble fertilizer and preparation method thereof - Google Patents
High-quality amino acid water-soluble fertilizer and preparation method thereof Download PDFInfo
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- CN110627562A CN110627562A CN201910818713.1A CN201910818713A CN110627562A CN 110627562 A CN110627562 A CN 110627562A CN 201910818713 A CN201910818713 A CN 201910818713A CN 110627562 A CN110627562 A CN 110627562A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention provides a high-quality amino acid water-soluble fertilizer and a preparation method thereof, which relate to the field of water-soluble fertilizers and comprise the following raw material components in parts by weight: 10-15 parts of enzymolysis bone meal, 2-5 parts of composite trace element components, 0.5-1 part of composite microbial agent, 1-2 parts of potassium fulvate, 0.4-0.8 part of surfactant, 0.1-0.5 part of alkyl glycoside quaternary ammonium salt, 0.1-0.5 part of alpha-alkenyl sodium sulfonate, 18-25 parts of water-soluble montmorillonite, 0.01-0.05 part of sodium tartrate and 80-100 parts of distilled water.
Description
Technical Field
The invention relates to the field of water-soluble fertilizers, in particular to a high-quality amino acid water-soluble fertilizer and a preparation method thereof.
Background
The development of agricultural production cannot be separated from fertilizers, excessive fertilizers are used for providing nutrients all over the world, so that the growth of plants is supported, and the types of the fertilizers and the nutritional ingredients contained in the fertilizers directly influence the yield and quality of agricultural products. With the continuous improvement of living standard of people, the requirements on the quality of agricultural products are also improved, and the quality of the agricultural products is also higher while the high yield is pursued.
Although chemical fertilizers have provided benefits for modern agriculture, excessive use of chemical fertilizers has resulted in soil hardening and corrosion, and has resulted in reduced yield and reduced efficiency of chemical fertilizers, which has resulted in problems such as low utilization of soil fertility, unbalanced nutrients, soil hardening, unbalanced soil microbial communities, environmental pollution, nutrient enrichment, and a series of environmental problems.
The Water-Soluble Fertilizer (WSF for short) is a multi-element compound Fertilizer capable of being completely dissolved in Water, can be used as new environment-protecting Fertilizer, and can be quickly dissolved in Water, and can be easily absorbed by crops, and its absorption utilization rate is relatively high, and more importantly, it can be used in the facilities agriculture of spray drip irrigation, etc. to implement Water-Fertilizer integration and attain the effect of saving Water, Fertilizer and labour.
Compared with the traditional calcium superphosphate, granulated compound fertilizer and other varieties, the water-soluble fertilizer has obvious advantages. The fertilizer is mainly characterized by small dosage, convenient use, low use cost, fast crop absorption and high utilization rate of nutrient components. Therefore, people can completely design a formula according to the characteristics of the nutritional requirements needed by the growth of crops, and the scientific formula can not cause fertilizer waste, so that the fertilizer utilization rate is not much lower than that of the conventional compound chemical fertilizer by 2-3 times.
And secondly, the water-soluble fertilizer is a quick-acting fertilizer, so that a grower can quickly see the effect and the expression of the fertilizer, and the fertilizer formula can be adjusted according to different growth vigors of crops at any time. Moreover, the application method of the water-soluble fertilizer is very simple and convenient, the water-soluble fertilizer can be applied in irrigation along with irrigation water in modes of sprinkling irrigation, drip irrigation and the like, so that not only is water saved, but also the fertilizer and labor are saved, and the benefit of using the water-soluble fertilizer at present with increasingly high labor cost is obvious.
The amino acid water-soluble fertilizer is prepared by taking amino acid as a main body and adding different elements according to the growth needs of plants. The amino acid water-soluble fertilizer is an important branch in water-soluble fertilizers, the amino acid water-soluble fertilizers in the current market are directly added with amino acid, so that the production cost is greatly increased, the effective content of the amino acid is extremely low, the solubility is poor, the amino acid water-soluble fertilizers are easy to separate out in the drip irrigation process, drip irrigation equipment is blocked and damaged, the separated amino acid water-soluble fertilizers are difficult to absorb by plants, and the utilization rate of nutrient components is reduced.
Disclosure of Invention
The invention aims to provide a high-quality amino acid water-soluble fertilizer and a preparation method thereof.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
10-15 parts of enzymolysis bone meal, 2-5 parts of composite trace element components, 0.5-1 part of composite microbial agent, 1-2 parts of potassium fulvate, 0.4-0.8 part of surfactant, 0.1-0.5 part of alkyl glycoside quaternary ammonium salt, 0.1-0.5 part of alpha-alkenyl sodium sulfonate, 18-25 parts of water-soluble montmorillonite, 0.01-0.05 part of sodium tartrate and 80-100 parts of distilled water.
Further, the high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
12 parts of enzymolysis bone meal, 4 parts of composite trace element components, 0.6 part of composite microbial agent, 1.5 parts of potassium fulvate, 0.7 part of surfactant, 0.3 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 22 parts of water-soluble montmorillonite, 0.02 part of sodium tartrate and 88 parts of distilled water.
Further, the high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
15 parts of enzymolysis bone meal, 2 parts of composite trace element components, 0.5 part of composite microbial agent, 1.5 parts of potassium fulvate, 0.4 part of surfactant, 0.2 part of alkyl glycoside quaternary ammonium salt, 0.2 part of alpha-alkenyl sodium sulfonate, 24 parts of water-soluble montmorillonite, 0.01 part of sodium tartrate and 100 parts of distilled water.
Further, the preparation method of the enzymatic bone meal comprises the following steps:
adding the animal bone powder into distilled water with the mass 10-15 times of that of the animal bone powder, uniformly stirring, adding a certain amount of compound protease BJ-0016-one 2887, carrying out enzymolysis for 5-10h under the condition of water bath at 50-55 ℃, recovering the room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and carrying out reduced pressure evaporation at 50-55 ℃ to dryness.
Further, the adding mass of the compound protease BJ-0016-2887 is 1-2% of the mass of the animal bone powder.
Furthermore, the aperture of the microfiltration membrane is 0.1-0.5 μm and the pressure is 0.1-0.2MPa during the microfiltration operation.
Further, the composite trace elements comprise the following components in parts by weight: 0.5-12 parts of zinc sulfate, 0.1-10 parts of copper sulfate, 0.1-5 parts of cobalt sulfate, 0.5-15 parts of ferric chloride, 0.1-10 parts of boric acid, 0.1-10 parts of manganese sulfate and 0.1-10 parts of ammonium molybdate.
Further, the compound microbial agent comprises 1-10 parts by weight of bacillus thuringiensis with the content of more than or equal to 1 x 108cfu/ml, 15-20 parts by weight of trichoderma with the content of more than or equal to 1 x 108cfu/ml, 3-6 parts by weight of bacillus licheniformis with the content of more than or equal to 1 x 108cfu/ml and 1-3 parts by weight of bacillus amyloliquefaciens with the content of more than or equal to 1 x 108 cfu/ml.
Further, the preparation method of the compound microbial agent comprises the following steps: taking the bacillus thuringiensis, the trichoderma, the bacillus licheniformis and the bacillus amyloliquefaciens out of liquid nitrogen, respectively putting the bacillus thuringiensis, the trichoderma, the bacillus licheniformis and the bacillus amyloliquefaciens into warm water at 37 ℃ to obtain bacterial liquid, inoculating the bacterial liquid into a sterilized LB liquid culture medium, horizontally placing the liquid for 30-50min, carrying out shake culture at the constant temperature of 37 ℃ and the rotating speed of 200r/min for 30-45h, stopping culture after the number of viable bacteria reaches 1 multiplied by 108cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
(1) adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 10-15min, heating to reflux treatment for 2-4h, and cooling to room temperature to obtain a chelating solution;
(2) sequentially adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution under stirring at the rotating speed of 100-150rpm, and continuing stirring for 20-50min after the addition is finished, and stopping stirring;
(3) dropwise adding the compound microbial agent, standing for 10-20min after dropwise adding, transferring to a reciprocating shaker, oscillating at 37 deg.C for 1-3h, with frequency of 80-100 times/min and amplitude of 50-100 mm;
(4) and finally, adding sodium tartrate, stirring at the rotating speed of 50-80rpm for 20-60min, and subpackaging.
The invention has the following beneficial effects:
the bone meal is rich in tricalcium phosphate and protein, the collagen and keratin which form collagen fibers are the highest in content, a plurality of amino acids can be obtained after hydrolysis is carried out on the bone meal by utilizing compound protease BJ-0016-2887, compared with the existing method of directly adding ready-made amino acids, the production cost can be greatly reduced, the problems that the amino acids in the existing commercially available amino acid water-soluble fertilizer are easy to lose, the effective content is extremely low, the dissolubility is poor and the like are solved, the amino acids in the enzymatically hydrolyzed bone meal are chelated with compound trace element components at high temperature, a cyclic structure product is formed by coordination reaction between 1 or more coordination groups in the amino acids and metal ions, the stability of the chelated structure in aqueous solution is improved compared with that of a single amino acid structure due to the formation of a chelated ring, and the positive effect is achieved on improving the fertilizer efficiency of the amino acid water-soluble fertilizer, so that the amino, The compound microbial inoculum is added, amino acid and nutrient components thereof can provide nutrient components for bacillus thuringiensis, trichoderma, bacillus licheniformis and bacillus amyloliquefaciens, the strains are ensured to propagate in soil, the strains can fully play the roles of dissolving phosphorus and potassium, promoting enzyme activity and the like, the fertilizer efficiency is improved in turn, the two have synergistic effect to jointly improve the yield,
the planting test shows that the compound microbial agent is not added, and the plant height, the single-plant fruit bearing number, the single-fruit quality and the yield per mu of the compound microbial agent are all lower than those of the amino acid water-soluble fertilizer added with the compound microbial agent.
Drawings
FIG. 1 is a flow chart of a preparation method of the high-quality amino acid water-soluble fertilizer.
Detailed Description
Example 1:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
12 parts of enzymolysis bone meal, 4 parts of composite trace element components, 0.6 part of composite microbial agent, 1.5 parts of potassium fulvate, 0.7 part of surfactant, 0.3 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 22 parts of water-soluble montmorillonite, 0.02 part of sodium tartrate and 88 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone powder into distilled water 15 times of the animal bone powder, stirring and mixing uniformly, adding composite protease BJ-0016-2887 accounting for 1.5% of the animal bone powder, carrying out enzymolysis for 8h in a water bath at 50 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 55 ℃, wherein the aperture of the microfiltration membrane is 0.2 mu m and the pressure is 0.2MPa during microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 10 parts of zinc sulfate, 5 parts of copper sulfate, 0.5 part of cobalt sulfate, 12 parts of ferric chloride, 0.2 part of boric acid, 0.5 part of manganese sulfate and 8 parts of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 1085 parts by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10818 parts by weight of cfu/ml trichoderma, content 1 x 1085 parts by weight of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 2 parts by weight.
The preparation method of the compound microbial agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 40min, performing shake culture at 37 deg.C and 180r/min for 40h until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 12min, heating to reflux treatment for 3h, cooling to room temperature to obtain a chelating solution, sequentially adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution under stirring at the rotating speed of 150rpm, continuing stirring for 30min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 20min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 2h at 37 ℃, carrying out frequency of 100 times/min and amplitude of 50mm, finally adding sodium tartrate, stirring at the rotating speed of 60rpm for 30min, and subpackaging.
Example 2:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
15 parts of enzymolysis bone meal, 2 parts of composite trace element components, 0.5 part of composite microbial agent, 1.5 parts of potassium fulvate, 0.4 part of surfactant, 0.2 part of alkyl glycoside quaternary ammonium salt, 0.2 part of alpha-alkenyl sodium sulfonate, 24 parts of water-soluble montmorillonite, 0.01 part of sodium tartrate and 100 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone meal into distilled water 15 times of the mass of the animal bone meal, uniformly stirring, adding 2887 compound protease BJ-0016 which accounts for 2% of the mass of the animal bone meal, carrying out enzymolysis for 10 hours in a water bath at 50 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 50 ℃, wherein the aperture of the microfiltration membrane is 0.4 mu m and the pressure is 0.2MPa during microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 1 part of zinc sulfate, 0.2 part of copper sulfate, 5 parts of cobalt sulfate, 10 parts of ferric chloride, 2 parts of boric acid, 10 parts of manganese sulfate and 5 parts of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 10810 parts by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10818 parts by weight of cfu/ml trichoderma, content 1 x 1083 parts of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 2 parts by weight.
Compound microorganism bacteriumThe preparation method of the agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 40min, performing shake culture at 37 deg.C and 180r/min for 40h until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 15min, heating to reflux treatment for 4h, cooling to room temperature to obtain a chelating solution, sequentially adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution under stirring at the rotating speed of 150rpm, continuing stirring for 40min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 15min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 2h at 37 ℃, carrying out frequency of 100 times/min and amplitude of 60mm, finally adding sodium tartrate, stirring at the rotating speed of 50rpm for 50min, and subpackaging.
Example 3:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
10 parts of enzymolysis bone meal, 4 parts of composite trace element components, 1 part of composite microbial agent, 1.2 parts of potassium fulvate, 0.8 part of surfactant, 0.2 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 20 parts of water-soluble montmorillonite, 0.02 part of sodium tartrate and 100 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone powder into distilled water 15 times of the animal bone powder, stirring and mixing uniformly, adding composite protease BJ-0016-2887 accounting for 1.2% of the animal bone powder, carrying out enzymolysis for 10h in a water bath at 50 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation to dryness at 50 ℃, wherein the aperture of the microfiltration membrane is 0.2 mu m and the pressure is 0.2MPa during microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 10 parts of zinc sulfate, 0.5 part of copper sulfate, 2 parts of cobalt sulfate, 10 parts of ferric chloride, 0.5 part of boric acid, 2 parts of manganese sulfate and 8 parts of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 1085 parts by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10820 parts of cfu/ml trichoderma, the content of which is 1 multiplied by 1085 parts by weight of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 2 parts by weight.
The preparation method of the compound microbial agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 40min, performing shake culture at 37 deg.C and 180r/min for 40h until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 12min, heating to reflux treatment for 4h, cooling to room temperature to obtain a chelating solution, sequentially adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution under stirring at the rotating speed of 150rpm, continuing stirring for 40min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 15min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 2h at 37 ℃, carrying out frequency of 100 times/min and amplitude of 60mm, finally adding sodium tartrate, stirring at the rotating speed of 80rpm for 30min, and subpackaging.
Example 4:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
15 parts of enzymolysis bone meal, 4 parts of composite trace element components, 0.6 part of composite microbial agent, 1.2 parts of potassium fulvate, 0.8 part of surfactant, 0.2 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 20 parts of water-soluble montmorillonite, 0.03 part of sodium tartrate and 100 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone meal into distilled water 15 times of the mass of the animal bone meal, uniformly stirring, adding 2887 compound protease BJ-0016 with the mass of 2% of the animal bone meal, carrying out enzymolysis for 8h in a water bath at 55 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation to dryness at 50 ℃, wherein the aperture of the microfiltration membrane is 0.2 mu m and the pressure is 0.2MPa during microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 10 parts of zinc sulfate, 5 parts of copper sulfate, 1 part of cobalt sulfate, 10 parts of ferric chloride, 2 parts of boric acid, 1 part of manganese sulfate and 10 parts of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 1085 parts by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10818 parts by weight of cfu/ml trichoderma, content 1 x 1084 parts of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 2 parts by weight.
The preparation method of the compound microbial agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 35min, performing shake culture at 37 deg.C and 180r/min for 45 hr until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 12min, heating to reflux treatment for 3h, cooling to room temperature to obtain a chelating solution, adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution in sequence under the stirring of 150rpm, continuing stirring for 35min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 20min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 2h at 37 ℃, carrying out frequency of 100 times/min and amplitude of 80mm, finally adding sodium tartrate, stirring for 50min at 60rpm, and subpackaging.
Example 5:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
10 parts of enzymolysis bone meal, 2 parts of compound trace element components, 0.5 part of compound microbial agent, 1 part of potassium fulvate, 0.4 part of surfactant, 0.1 part of alkyl glycoside quaternary ammonium salt, 0.1 part of alpha-alkenyl sodium sulfonate, 18 parts of water-soluble montmorillonite, 0.01 part of sodium tartrate and 80 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone meal into distilled water with the mass 10 times of that of the animal bone meal, uniformly stirring, adding 2887 compound protease BJ-0016 with the mass 1% of the animal bone meal, carrying out enzymolysis for 5h in a water bath at 50 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 50 ℃, wherein the aperture of the microfiltration membrane is 0.1 mu m and the pressure is 0.1MPa during the microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 0.5 part of zinc sulfate, 0.1 part of copper sulfate, 0.1 part of cobalt sulfate, 0.5 part of ferric chloride, 0.1 part of boric acid, 0.1 part of manganese sulfate and 0.1 part of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 1081 part by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10815 parts by weight of cfu/ml trichoderma, content of 1 multiplied by 1083 parts of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 1 part by weight.
The preparation method of the compound microbial agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 30min, performing shake culture at constant temperature of 37 deg.C and rotation speed of 150r/min for 30h until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 10min, heating to reflux treatment for 2h, cooling to room temperature to obtain a chelating solution, adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution in sequence under stirring at the rotating speed of 100rpm, continuing stirring for 20min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 10min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 1h at 37 ℃, carrying out frequency of 80 times/min and amplitude of 50mm, finally adding sodium tartrate, stirring at the rotating speed of 50rpm for 20min, and subpackaging.
Example 6:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
15 parts of enzymolysis bone meal, 5 parts of composite trace element components, 1 part of composite microbial agent, 2 parts of potassium fulvate, 0.8 part of surfactant, 0.5 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 25 parts of water-soluble montmorillonite, 0.05 part of sodium tartrate and 100 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone meal into distilled water 15 times of the mass of the animal bone meal, uniformly stirring, adding 2887 compound protease BJ-0016 containing 2% of the mass of the animal bone meal, carrying out enzymolysis for 10h in a water bath at 55 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 55 ℃, wherein the aperture of the microfiltration membrane is 0.5 mu m and the pressure is 0.2MPa during the microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 12 parts of zinc sulfate, 10 parts of copper sulfate, 5 parts of cobalt sulfate, 15 parts of ferric chloride, 10 parts of boric acid, 10 parts of manganese sulfate and 10 parts of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 10810 parts by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10820 parts of cfu/ml trichoderma, the content of which is 1 multiplied by 1086 parts of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 3 parts by weight.
Preparation of compound microbial agentThe method comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 50min, performing shake culture at 37 deg.C and 200r/min for 45 hr until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 15min, heating to reflux treatment for 4h, cooling to room temperature to obtain a chelating solution, sequentially adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution under stirring at the rotating speed of 150rpm, continuing stirring for 50min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 20min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 3h at 37 ℃, carrying out frequency of 100 times/min and amplitude of 100mm, finally adding sodium tartrate, stirring at the rotating speed of 80rpm for 60min, and subpackaging.
Example 7:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
10 parts of enzymolysis bone meal, 5 parts of composite trace element components, 0.5 part of composite microbial agent, 2 parts of potassium fulvate, 0.4 part of surfactant, 0.5 part of alkyl glycoside quaternary ammonium salt, 0.1 part of alpha-alkenyl sodium sulfonate, 25 parts of water-soluble montmorillonite, 0.01 part of sodium tartrate and 100 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone meal into distilled water of which the mass is 10 times of that of the animal bone meal, uniformly stirring, adding 2887 compound protease BJ-0016 which accounts for 2 percent of the mass of the animal bone meal, carrying out enzymolysis for 10 hours in a water bath at 50 ℃, recovering the room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 50 ℃, wherein the aperture of a microfiltration membrane is 0.5 mu m and the pressure is 0.1MPa during microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 12 parts of zinc sulfate, 0.1 part of copper sulfate, 5 parts of cobalt sulfate, 0.5 part of ferric chloride, 10 parts of boric acid, 0.1 part of manganese sulfate and 10 parts of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 1081 part by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10820 parts of cfu/ml trichoderma, the content of which is 1 multiplied by 1083 parts of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 3 parts by weight.
The preparation method of the compound microbial agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 30min, performing shake culture at 37 deg.C and 200r/min for 30h until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 15min, heating to reflux treatment for 2h, cooling to room temperature to obtain a chelating solution, adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution in sequence under the stirring of 150rpm, continuing stirring for 20min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 20min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 1h at 37 ℃, carrying out frequency of 100 times/min and amplitude of 50mm, finally adding sodium tartrate, stirring at 80rpm for 20min, and subpackaging.
Example 8:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
15 parts of enzymolysis bone meal, 2 parts of composite trace element components, 1 part of composite microbial agent, 1 part of potassium fulvate, 0.8 part of surfactant, 0.1 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 18 parts of water-soluble montmorillonite, 0.05 part of sodium tartrate and 80 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone meal into distilled water 15 times of the mass of the animal bone meal, uniformly stirring, adding 2887 compound protease BJ-0016 containing 1% of the mass of the animal bone meal, carrying out enzymolysis for 5 hours in a water bath at 55 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 55 ℃, wherein the aperture of the microfiltration membrane is 0.1 mu m and the pressure is 0.2MPa during the microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 0.5 part of zinc sulfate, 10 parts of copper sulfate, 0.1 part of cobalt sulfate, 15 parts of ferric chloride, 0.1 part of boric acid, 10 parts of manganese sulfate and 0.1 part of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 10810 parts by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10815 parts by weight of cfu/ml trichoderma, content of 1 multiplied by 1083 parts of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 3 parts by weight.
The preparation method of the compound microbial agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 30min, performing shake culture at 37 deg.C and 200r/min for 30h until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 15min, heating to reflux treatment for 2h, cooling to room temperature to obtain a chelating solution, adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution in sequence under the stirring of 150rpm, continuing stirring for 20min after the addition is finished, stopping stirring, dropwise adding the compound microbial agent, standing for 20min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 1h at 37 ℃, carrying out frequency of 100 times/min and amplitude of 50mm, finally adding sodium tartrate, stirring at 80rpm for 20min, and subpackaging.
Comparative example 1:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
12 parts of enzymolysis bone meal, 4 parts of composite trace element components, 0.6 part of composite microbial agent, 1.5 parts of potassium fulvate, 0.7 part of surfactant, 0.3 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 22 parts of water-soluble montmorillonite, 0.02 part of sodium tartrate and 88 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone powder into distilled water 15 times of the animal bone powder, stirring and mixing uniformly, adding composite protease BJ-0016-2887 accounting for 1.5% of the animal bone powder, carrying out enzymolysis for 8h in a water bath at 50 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 55 ℃, wherein the aperture of the microfiltration membrane is 0.2 mu m and the pressure is 0.2MPa during microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 10 parts of zinc sulfate, 5 parts of copper sulfate, 0.5 part of cobalt sulfate, 12 parts of ferric chloride, 0.2 part of boric acid, 0.5 part of manganese sulfate and 8 parts of ammonium molybdate.
Wherein the content of the compound microbial agent is 1 multiplied by 1085 parts by weight of cfu/ml bacillus thuringiensis with the content of 1 multiplied by 10818 parts by weight of cfu/ml trichoderma, content 1 x 1085 parts by weight of cfu/ml bacillus licheniformis with the content of 1 multiplied by 108cfu/ml of bacillus amyloliquefaciens 2 parts by weight.
The preparation method of the compound microbial agent comprises the following steps: taking Bacillus thuringiensis, Trichoderma, Bacillus licheniformis and Bacillus amyloliquefaciens out of liquid nitrogen, respectively placing into 37 deg.C warm water to obtain bacterial liquid, inoculating the bacterial liquid into sterilized LB liquid culture medium, horizontally placing for 40min, performing shake culture at 37 deg.C and 180r/min for 40h until viable count reaches 1 × 108Stopping culturing after cfu/ml, and mixing.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic hydrolysis bone meal, the composite trace element components, the potassium fulvate, the surfactant, the alkyl glycoside quaternary ammonium salt, the alpha-alkenyl sodium sulfonate and the water-soluble montmorillonite into distilled water, stirring for 30min after the addition is finished, stopping stirring, dropwise adding the composite microbial agent, standing for 20min after the dropwise addition is finished, transferring to a reciprocating shaker, oscillating for 2h at 37 ℃, oscillating for 100 times/min at frequency and 50mm in amplitude, finally adding the sodium tartrate, stirring for 30min at the rotating speed of 60rpm, and subpackaging.
Comparative example 1 is substantially the same as example 1 except that the enzymolyzed bone meal and the composite trace element component do not undergo a chelation reaction in advance.
Comparative example 2:
a high-quality amino acid water-soluble fertilizer comprises the following raw material components in parts by weight:
12 parts of enzymolysis bone meal, 4 parts of composite trace element components, 1.5 parts of potassium fulvate, 0.7 part of surfactant, 0.3 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 22 parts of water-soluble montmorillonite, 0.02 part of sodium tartrate and 88 parts of distilled water.
The preparation method of the enzymatic bone meal comprises the following steps:
adding animal bone powder into distilled water 15 times of the animal bone powder, stirring and mixing uniformly, adding composite protease BJ-0016-2887 accounting for 1.5% of the animal bone powder, carrying out enzymolysis for 8h in a water bath at 50 ℃, recovering to room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and then carrying out reduced pressure evaporation at 55 ℃, wherein the aperture of the microfiltration membrane is 0.2 mu m and the pressure is 0.2MPa during microfiltration operation.
The composite trace elements comprise the following components in parts by weight: 10 parts of zinc sulfate, 5 parts of copper sulfate, 0.5 part of cobalt sulfate, 12 parts of ferric chloride, 0.2 part of boric acid, 0.5 part of manganese sulfate and 8 parts of ammonium molybdate.
The preparation method of the high-quality amino acid water-soluble fertilizer comprises the following steps:
adding the enzymatic hydrolyzed bone meal, the composite trace element ingredients, the potassium fulvate, the surfactant, the alkyl glycoside quaternary ammonium salt, the alpha-alkenyl sodium sulfonate and the water-soluble montmorillonite into distilled water, stirring for 30min after the addition is finished, stopping stirring, dropwise adding the distilled water, standing for 20min after the dropwise addition is finished, transferring to a reciprocating shaking table, oscillating for 2h at 37 ℃, oscillating for 100 times/min at a frequency of 100 and with an amplitude of 50mm, finally adding the sodium tartrate, stirring for 30min at a rotating speed of 60rpm, and subpackaging.
Comparative example 1 is substantially the same as example 1 except that an equal amount of distilled water is used instead of the complex microbial inoculant.
And (3) detecting the content of amino acid in the amino acid water-soluble fertilizer:
referring to GB 5009.124-2016 (determination of amino acids in food), the content of various amino acids in the amino acid water-soluble fertilizer in the examples 1-3 is determined by a Biochrom 30+ full-automatic amino acid analyzer, and the chromatographic conditions are as follows: the pump 1: (buffer solution) flow rate: 0-130ml/hr, pressure 45 MPa, pump 1: (ninhydrin) flow rate: 0-50 ml/hr, pressure 45 MPa, packing: 8 um cation exchange, the results are shown in Table 1 below:
table 1:
| amino acid type | EXAMPLE 1 amino acid content in amino acid Water-soluble Fertilizer (μ g/mL) | Example 2 amino acid content in amino acid Water soluble Fertilizer (μ g/mL) | EXAMPLE 3 amino acid content in amino acid Water-soluble Fertilizer (μ g/mL) |
| Aspartic acid | 83.14 | 80.65 | 81.44 |
| Threonine | 26.62 | 22.28 | 27.50 |
| Serine | 15.96 | 16.66 | 15.23 |
| Glutamic acid | 110.65 | 111.71 | 121.24 |
| Proline | 44.83 | 45.65 | 42.33 |
| Glycine | 174.26 | 153.38 | 175.80 |
| Cysteine | 20.88 | 21.32 | 22.36 |
| Valine | 86.37 | 88.20 | 81.54 |
| Methionine | 10.05 | 11.95 | 10.02 |
| Leucine | 55.28 | 42.73 | 50.21 |
| Isoleucine | 24.73 | 25.85 | 20.15 |
| Phenylalanine | 115.36 | 120.23 | 112.03 |
| Lysine | 48.83 | 52.69 | 45.54 |
| Arginine | 29.34 | 40.36 | 33.60 |
As can be seen from the above table 1, the amino acid water-soluble fertilizer contains various amino acids, wherein the contents of glutamic acid, glycine and phenylalanine all reach 100 μ g/mL, and the growth requirements of crops can be completely met.
Planting experiment:
the test is carried out in Pukou Shifu village of Nanjing in 6 months in 2018, pepper seeds are soaked for germination, the pepper seeds are sowed in nutrient soil of a seedling tray after white emergence, and the pepper seeds are transplanted into a test field when the seedlings grow to 5 leaves and 1 heart, and 4000 strains are planted in each mu of field. The test fields were 5 groups, and the test fields were each applied with the amino acid water-soluble fertilizer (brand: xingnohuai, amino acid foliar fertilizer) sold in the market, example 1, example 2, example 3, comparative example 1, comparative example 2, and clear water, which were designated as test group 1, test group 2, test group 3, comparative group 1, comparative group 2, comparative group 3, and blank group, respectively.
The variety of the tested pepper is Guizhou pepper No. 8, the growth vigor is neat and consistent, the field management level is higher, and the spraying time is respectively selected in the bud development period and the initial flowering period of the pepper until obvious dew appears on leaf surfaces. During the test period, the weather is mainly sunny or cloudy, no rainfall occurs within 2-3d after spraying, and the final harvest condition is shown in the following table 2:
table 2:
| plant height/cm | Number of fruits per plant | Mass per gram of single fruit | Yield/(kilogram/mu) | |
| Experimental group 1 | 58.32±0.25 | 36.82±0.66 | 108.64±2.11 | 2562 |
| Experimental group 2 | 58.68±0.38 | 37.14±0.57 | 109.02±3.00 | 2605 |
| Experimental group 3 | 58.63±0.27 | 37.14±0.34 | 108.93±2.65 | 2588 |
| Comparative group 1 | 51.64±0.43 | 34.72±0.31 | 97.44±2.03 | 1823 |
| Comparative group 2 | 51.06±0.22 | 33.90±1.02 | 94.32±1.84 | 1553 |
| Comparative group 3 | 51.55±0.24 | 35.00±0.56 | 94.96±0.36 | 1636 |
| Blank group | 33.08±0.50 | 19.56±0.24 | 47.73±1.22 | 682 |
As can be seen from table 2 above: in the preparation process, the enzymatic bone meal and the compound trace element components are subjected to chelation reaction in advance, so that the fertilizer effect of the amino acid water-soluble fertilizer is improved, and the reason for the chelation is that the amino acid components are not easy to lose and are easy to be absorbed by crops.
In addition, the compound microbial inoculum also plays a positive role, the combination of the compound microbial inoculum and other components can greatly improve the fertilizer efficiency, and the compound microbial inoculum is not added in the comparative example 2, so that the plant height, the single plant fruit bearing number, the single fruit quality and the per mu yield of the compound microbial inoculum are all lower than those of the experimental group added with the compound microbial inoculum in the examples 1-3.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. The high-quality amino acid water-soluble fertilizer is characterized by comprising the following raw material components in parts by weight:
10-15 parts of enzymolysis bone meal, 2-5 parts of composite trace element components, 0.5-1 part of composite microbial agent, 1-2 parts of potassium fulvate, 0.4-0.8 part of surfactant, 0.1-0.5 part of alkyl glycoside quaternary ammonium salt, 0.1-0.5 part of alpha-alkenyl sodium sulfonate, 18-25 parts of water-soluble montmorillonite, 0.01-0.05 part of sodium tartrate and 80-100 parts of distilled water.
2. The high-quality amino acid water-soluble fertilizer as claimed in claim 1, which comprises the following raw materials in parts by weight:
12 parts of enzymolysis bone meal, 4 parts of composite trace element components, 0.6 part of composite microbial agent, 1.5 parts of potassium fulvate, 0.7 part of surfactant, 0.3 part of alkyl glycoside quaternary ammonium salt, 0.5 part of alpha-alkenyl sodium sulfonate, 22 parts of water-soluble montmorillonite, 0.02 part of sodium tartrate and 88 parts of distilled water.
3. The high-quality amino acid water-soluble fertilizer as claimed in claim 1, which comprises the following raw materials in parts by weight:
15 parts of enzymolysis bone meal, 2 parts of composite trace element components, 0.5 part of composite microbial agent, 1.5 parts of potassium fulvate, 0.4 part of surfactant, 0.2 part of alkyl glycoside quaternary ammonium salt, 0.2 part of alpha-alkenyl sodium sulfonate, 24 parts of water-soluble montmorillonite, 0.01 part of sodium tartrate and 100 parts of distilled water.
4. The high-quality amino acid water-soluble fertilizer as claimed in claim 1, wherein the preparation method of the enzymatic hydrolyzed bone meal comprises the following steps:
adding the animal bone powder into distilled water with the mass 10-15 times of that of the animal bone powder, uniformly stirring, adding a certain amount of compound protease BJ-0016-one 2887, carrying out enzymolysis for 5-10h under the condition of water bath at 50-55 ℃, recovering the room temperature, filtering by using filter paper, carrying out microfiltration on the filtrate, and carrying out reduced pressure evaporation at 50-55 ℃ to dryness.
5. The high-quality amino acid water-soluble fertilizer as claimed in claim 4, wherein the added mass of the compound protease BJ-0016-2887 is 1-2% of the mass of animal bone meal.
6. The high-quality amino acid water-soluble fertilizer according to claim 4, wherein the microfiltration membrane has a pore size of 0.1 to 0.5 μm and a pressure of 0.1 to 0.2MPa during microfiltration.
7. The high-quality amino acid water-soluble fertilizer as claimed in claim 1, wherein the composite trace elements comprise the following components in parts by weight: 0.5-12 parts of zinc sulfate, 0.1-10 parts of copper sulfate, 0.1-5 parts of cobalt sulfate, 0.5-15 parts of ferric chloride, 0.1-10 parts of boric acid, 0.1-10 parts of manganese sulfate and 0.1-10 parts of ammonium molybdate.
8. The high-quality amino acid water-soluble fertilizer as claimed in claim 1, wherein the content of the compound microbial agent is more than or equal to 1 x 1081-10 parts of cfu/ml bacillus thuringiensis with the content being more than or equal to 1 multiplied by 10815-20 parts of cfu/ml trichoderma, the content is more than or equal to 1 multiplied by 1083-6 parts of cfu/ml bacillus licheniformis with the content being more than or equal to 1 multiplied by 1081-3 parts of cfu/ml bacillus amyloliquefaciens.
9. The high-quality amino acid water-soluble fertilizer according to claim 8, wherein the compound microbial agent is prepared by the following steps: taking the bacillus thuringiensis, the trichoderma, the bacillus licheniformis and the bacillus amyloliquefaciens out of liquid nitrogen, respectively putting the bacillus thuringiensis, the trichoderma, the bacillus licheniformis and the bacillus amyloliquefaciens into warm water at 37 ℃ to obtain bacterial liquid, inoculating the bacterial liquid into a sterilized LB liquid culture medium, horizontally placing the liquid for 30-50min, carrying out shake culture at the constant temperature of 37 ℃ and the rotating speed of 200r/min for 30-45h, stopping culture after the number of viable bacteria reaches 1 multiplied by 108cfu/ml, and mixing.
10. A preparation method of the high-quality amino acid water-soluble fertilizer as defined in any one of claims 1 to 9, which comprises the following steps:
(1) adding the enzymatic bone meal and the compound trace element components into distilled water, carrying out ultrasonic oscillation treatment for 10-15min, heating to reflux treatment for 2-4h, and cooling to room temperature to obtain a chelating solution;
(2) sequentially adding potassium fulvate, surfactant, alkyl glycoside quaternary ammonium salt, alpha-alkenyl sodium sulfonate and water-soluble montmorillonite into the chelating solution under stirring at the rotating speed of 100-150rpm, and continuing stirring for 20-50min after the addition is finished, and stopping stirring;
(3) dropwise adding the compound microbial agent, standing for 10-20min after dropwise adding, transferring to a reciprocating shaker, oscillating at 37 deg.C for 1-3h, with frequency of 80-100 times/min and amplitude of 50-100 mm;
(4) and finally, adding sodium tartrate, stirring at the rotating speed of 50-80rpm for 20-60min, and subpackaging.
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