CN110627224A - Formula and preparation method of water quality purifying agent for bog red pseudo single-package flora - Google Patents
Formula and preparation method of water quality purifying agent for bog red pseudo single-package flora Download PDFInfo
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- CN110627224A CN110627224A CN201910934814.5A CN201910934814A CN110627224A CN 110627224 A CN110627224 A CN 110627224A CN 201910934814 A CN201910934814 A CN 201910934814A CN 110627224 A CN110627224 A CN 110627224A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
Abstract
The invention discloses a swamp red pseudo single-package flora water purifying agent which comprises the following components in percentage by weight: 0.5% of sodium acetate, 0.3% of ammonium chloride, 0.03% of magnesium sulfate, 0.07% of monopotassium phosphate, 0.04% of dipotassium phosphate, 0.06% of salt, 0.04% of beef extract, 0.02% of yeast extract, 0.5% of baking soda, 0.007% of riboflavin, 5% -10% of rhodopseudomonas palustris strain and the balance of pure water; the method has the advantages of reducing the content of total ammonia and nitrite nitrogen in the culture water body, promoting the growth of beneficial algae, inhibiting the propagation of harmful algae, reducing or eliminating algal toxins and the like; the invention also discloses a preparation method of the swamp red pseudo single-package flora water quality purifying agent, which comprises the steps of disinfecting and cleaning a production device, weighing raw materials (without swamp red pseudo single-package flora strains) according to the required weight of each component, adding the raw materials into purified water, fully stirring to dissolve and uniformly mix the components, inoculating the swamp red pseudo single-package flora strains with the required weight, stirring for at least 20 minutes, and finally filling and illuminating.
Description
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a formula of a water purifying agent for bog red pseudo single-package flora and a preparation method thereof.
Background
With the implementation of the aquatic product market admission system, the production of pollution-free aquatic products is a necessary choice for aquaculture. The pollution-free aquatic product cultivation requires fresh water quality and also meets the requirements of No-pollution food fresh water cultivation water quality (NY 5051-2001). Therefore, in the process of aquaculture, the good water quality standard in aquaculture is firstly known, the main parameters related to the quality of water quality and the functions of the main parameters are mastered, and the regulation and control technology aiming at the corresponding parameters is particularly important for ensuring the aquaculture safety and the product safety.
In modern aquaculture, the water quality standard provides that the water quality has the advantages of 'fat, alive, tender and cool', the four characters of 'fat, alive, tender and cool' are the experiences for judging the quality of the water quality summarized in long-term production practice, and the total requirement of the aquaculture water quality is to create conditions for keeping the aquaculture water 'fat, alive, tender and cool' all the time. The 'fertilizer' is that the pond water contains rich organic matters and various nutrient salts, and the water fertility is expressed by the water transparency in production, wherein the transparency is 25 cm-30 cm, the number of reproduced plankton is large, and particularly, the types of plankton which is easy to digest are large. "live" is the continuous and rapid transformation of all the materials in the pond, including living and non-living organisms, to form the dietary material cycle of the pond ecosystem. The water color of the pool is reflected in the change of the pool water according to different illumination. It means that the color and transparency of water are frequently changed, and the activity is caused by the change of organisms in the water. "tender" refers to water which is not old but is fat, the water is old and mainly has several colors of blue-green, rust, grey brown and milky white, and from the biological point of view, "tender" means that the algae in the water is in the growth period and the cells are not aged. "clear" means that the water is fresh and not turbid, and means that no other suspended matters or less suspended matters except plankton exist in the water, the transparency is more than 20 cm-25 cm, and the dissolved oxygen in the water is higher.
In addition, the pH value of the aquaculture water body is also required, and the optimal pH value of the general freshwater aquaculture water body is 7.5-8.5, namely the freshwater aquaculture water body is preferably neutral or slightly alkaline. The change of pH can directly affect the charged state of colloidal substances in the water body and the balance of ammonia and ammonia ions in the water, thereby leading the colloids to have adsorption and release effects on specific ions in the water, and affecting the effect of using fertilizers in ponds and the utilization of humus by fishes and shrimps. The peracid water quality can inhibit the nitrification process in water, so that the decomposition of organic matters is slow, the photosynthesis is weak, the metabolism of a culture object is slow, the feeding and digestion capacities are weakened, the activity is not favored, and the growth speed is influenced.
Furthermore, the total ammonia and nitrite nitrogen contents of the aquaculture water body are also required, and the total ammonia content of the aquaculture water body is generally required to be not more than 2 mg/L, and the nitrite nitrogen content is not more than 0.2 mg/L; for the culture of special aquatic products, the requirements are more strict, the total ammonia content of the culture water body is controlled below 1 mg/L, and the nitrite nitrogen content is controlled below 0.1 mg/L. The total ammonia and nitrite nitrogen are formed by decomposing organic matters, and the water quality is fertile, the more organic matters in the water are, and the total ammonia and nitrite nitrogen are also higher. And the total ammonia and nitrite nitrogen are toxic to aquatic animals, and slightly affect the growth and seriously endanger the survival.
Disclosure of Invention
The invention aims to provide a formula of a water quality purifying agent for bog red pseudo single-package floras, and the water quality purifying agent of the formula has the advantages of stabilizing the 'fertilizer, activity, tenderness and comfort' of an aquaculture water body, stabilizing the dissolved oxygen amount of the aquaculture water body, stabilizing the pH value of the aquaculture water body and reducing the total ammonia content and the nitrite nitrogen content in the aquaculture water body, and meanwhile, the invention discloses a preparation method of the water quality purifying agent of the formula.
The invention is realized by the following steps: a swamp red pseudo single-package flora water purifying agent comprises the following components in percentage by weight: 0.5 percent of sodium acetate, 0.3 percent of ammonium chloride, 0.03 percent of magnesium sulfate, 0.07 percent of monopotassium phosphate, 0.04 percent of dipotassium phosphate, 0.06 percent of salt, 0.04 percent of beef extract, 0.02 percent of yeast extract, 0.5 percent of baking soda, 0.007 percent of riboflavin, 5 to 10 percent of bog brown monascus strain and the balance of pure water.
Further, the bog red pseudo single-package strain is a third-generation strain obtained by sequentially culturing the first-generation strain in a laboratory.
Further, the finished product is reddish brown liquid, and the effective viable count is more than or equal to 5 multiplied by 108 CFU/ml。
A water quality purifying agent of bog red single-package flora is prepared through disinfecting and cleaning the apparatus, weighing raw materials (not containing bog red single-package bacterial strain) according to the weight needed by each component, adding them to purified water, stirring to dissolve and mix them uniformly, inoculating bog red single-package bacterial strain, stirring for at least 20 min, loading in containers and lighting.
Furthermore, transparent polyethylene barrels are adopted for filling, and the illumination time is not less than 24 hours.
Further, when the rhodopseudomonas palustris strain is cultured, the raw materials of the culture medium comprise ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate, yeast extract and distilled water, wherein the addition amount of the ammonium chloride, the dipotassium hydrogen phosphate, the sodium bicarbonate and the yeast extract is 1.5g, 0.4g, 2g and 3g in sequence per 100mL of the culture medium, and the balance is distilled water.
Furthermore, when the enlarged culture of the rhodopseudomonas palustris is carried out, the inoculation amount of the rhodopseudomonas palustris is 20-25%.
Further, when the Rhodopseudomonas palustris strain is cultured, the pH value of the culture medium is 7-7.5.
Further, when the rhodopseudomonas palustris strain is cultured, the culture temperature is 30-35 ℃, the illumination intensity is 2000Lx, and the culture time is 48 hours.
Compared with the prior art, the invention has the beneficial effects that:
(1) the ammonia nitrogen, nitrite, hydrogen sulfide and the like in the water are converted, the toxins such as ammonia, sulfur and the like are reduced or eliminated, and the content of total ammonia and nitrite nitrogen in the aquaculture water body is reduced.
(2) Can promote the growth of beneficial algae, inhibit the propagation of harmful algae, and reduce or eliminate algal toxins.
(3) Can stabilize the 'fertilizer, activity, tenderness and comfort' of the aquaculture water body, stabilize the dissolved oxygen of the aquaculture water body, stabilize the pH value of the aquaculture water body and inhibit the growth and the propagation of harmful organisms such as vibrios and the like.
(4) Can optimize water body nutrition, and promote cultivation of rotifer and other natural initial feed.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a process flow chart of the preparation method of the swamp red pseudo single-package flora water quality purifying agent of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
The first embodiment is as follows: a swamp red pseudo single-package flora water purifying agent comprises the following components in percentage by weight: 0.5% of sodium acetate, 0.3% of ammonium chloride, 0.03% of magnesium sulfate, 0.07% of monopotassium phosphate, 0.04% of dipotassium phosphate, 0.06% of salt, 0.04% of beef extract, 0.02% of yeast extract, 0.5% of baking soda, 0.007% of riboflavin, 5% -10% of bog red pseudo single-package bacteria strain, wherein the bog red pseudo single-package bacteria strain selects the third generation strain obtained by sequentially culturing the laboratory first generation strain, and the balance is pure water, and the normal quality parameters of the finished product of the water purifying agent comprise: the color is reddish brown liquid, and the effective viable count is more than or equal to 5 multiplied by 108 CFU/ml.
Referring to fig. 1, a preparation method of a marshland pseudomonad water quality purifying agent comprises the steps of disinfecting and cleaning a production device, weighing raw materials (without marshland pseudomonad strains) according to the required weight of each component, adding the raw materials into purified water, fully stirring to dissolve and uniformly mix the components, inoculating the marshland pseudomonad strains with the required weight, stirring for 20 minutes, filling and illuminating, wherein a transparent polyethylene barrel is adopted for filling to ensure light transmittance, and the illumination time is 24 hours.
The culture method of the rhodopseudomonas palustris strain used in the preparation method adopts a culture medium, wherein the raw materials of the culture medium comprise ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate, yeast extract and distilled water, the addition amounts of the ammonium chloride, the dipotassium hydrogen phosphate, the sodium bicarbonate and the yeast extract in each 100mL of the culture medium are 1.5g, 0.4g, 2g and 3g in sequence, the balance is distilled water, the pH value of the culture medium is 7, the culture temperature is 30 ℃, the illumination intensity is 2000Lx, the culture time is 48 hours, and the inoculation amount of the rhodopseudomonas palustris is 20% when the rhodopseudomonas palustris is subjected to enlarged culture.
Example two: a swamp red pseudo single-package flora water purifying agent comprises the following components in percentage by weight: 0.5% of sodium acetate, 0.3% of ammonium chloride, 0.03% of magnesium sulfate, 0.07% of monopotassium phosphate, 0.04% of dipotassium phosphate, 0.06% of salt, 0.04% of beef extract, 0.02% of yeast extract, 0.5% of baking soda, 0.007% of riboflavin, 5% -10% of bog red pseudo single-package bacteria strain, wherein the bog red pseudo single-package bacteria strain selects the third generation strain obtained by sequentially culturing the laboratory first generation strain, and the balance is pure water, and the normal quality parameters of the finished product of the water purifying agent comprise: the color is reddish brown liquid, and the effective viable count is more than or equal to 5 multiplied by 108 CFU/ml.
Referring to fig. 1, a preparation method of a marshland pseudomonad water quality purifying agent comprises the steps of disinfecting and cleaning a production device, weighing raw materials (without marshland pseudomonad strains) according to the required weight of each component, adding the raw materials into purified water, fully stirring to dissolve and uniformly mix the components, inoculating the marshland pseudomonad strains with the required weight, stirring for 25 minutes, finally filling and illuminating, wherein a transparent polyethylene barrel is adopted for filling to ensure light transmittance, and the illumination time is 30 hours.
The culture method of the rhodopseudomonas palustris strain used in the preparation method adopts a culture medium, wherein the raw materials of the culture medium comprise ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate, yeast extract and distilled water, the addition amounts of the ammonium chloride, the dipotassium hydrogen phosphate, the sodium bicarbonate and the yeast extract in each 100mL of the culture medium are 1.5g, 0.4g, 2g and 3g in sequence, the balance is distilled water, the pH value of the culture medium is 7.2, the culture temperature is 33 ℃, the illumination intensity is 2000Lx, the culture time is 48 hours, and the inoculation amount of the rhodopseudomonas palustris is 23% when the rhodopseudomonas palustris is subjected to enlarged culture.
Example three: a swamp red pseudo single-package flora water purifying agent comprises the following components in percentage by weight: 0.5% of sodium acetate, 0.3% of ammonium chloride, 0.03% of magnesium sulfate, 0.07% of monopotassium phosphate, 0.04% of dipotassium phosphate, 0.06% of salt, 0.04% of beef extract, 0.02% of yeast extract, 0.5% of baking soda, 0.007% of riboflavin, 5% -10% of bog red pseudo single-package bacteria strain, wherein the bog red pseudo single-package bacteria strain selects the third generation strain obtained by sequentially culturing the laboratory first generation strain, and the balance is pure water, and the normal quality parameters of the finished product of the water purifying agent comprise: the color is reddish brown liquid, and the effective viable count is more than or equal to 5 multiplied by 108 CFU/ml.
Referring to fig. 1, a preparation method of a marshland pseudomonad water quality purifying agent comprises the steps of disinfecting and cleaning a production device, weighing raw materials (without marshland pseudomonad strains) according to the required weight of each component, adding the raw materials into purified water, fully stirring to dissolve and uniformly mix the components, inoculating the marshland pseudomonad strains with the required weight, stirring for 30 minutes, filling and illuminating, wherein a transparent polyethylene barrel is adopted for filling to ensure light transmittance, and the illumination time is 36 hours.
The culture of the rhodopseudomonas palustris strain used in the preparation method adopts a culture medium, wherein the raw materials of the culture medium comprise ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate, yeast extract and distilled water, the addition amounts of the ammonium chloride, the dipotassium hydrogen phosphate, the sodium bicarbonate and the yeast extract in each 100mL of the culture medium are 1.5g, 0.4g, 2g and 3g in sequence, the balance is distilled water, the pH value of the culture medium is 7.5, the culture temperature is 35 ℃, the illumination intensity is 2000Lx, the culture time is 48 hours, and the inoculation amount of the rhodopseudomonas palustris is 25% when the rhodopseudomonas palustris is subjected to enlarged culture.
The rhodopseudomonas palustris belongs to one kind of photosynthetic bacteria, has protein content as high as 65%, is rich in various bioactive substances, has the characteristics of strong adaptability, high tolerance to high-concentration organic wastewater and strong decomposition and conversion capacity, certain tolerance and decomposition capacity to toxicants such as phenol, cyanogen and the like, and can be used for occasions such as water quality purification, sewage treatment and the like. The invention discloses a swamp red pseudo single-package flora water quality purifying agent, which is an application of swamp red pseudomonas in water quality purification treatment, and has the following advantages: firstly, ammonia nitrogen, nitrite, hydrogen sulfide and the like in water are converted, toxins such as ammonia sulfur and the like are reduced or eliminated, and the content of total ammonia and nitrite nitrogen in the aquaculture water body is reduced; secondly, the growth of beneficial algae can be promoted, the propagation of harmful algae can be inhibited, and algal toxins can be reduced or eliminated; thirdly, the fertilizer, the vitality, the tenderness and the coolness of the culture water body can be stabilized, the dissolved oxygen of the culture water body is stabilized, the pH value of the culture water body is stabilized, and the growth and the propagation of harmful organisms such as vibrios and the like are inhibited; and fourthly, the nutrition of the water body can be optimized, and the growth promoting effect on the natural initial feed such as the rotifer is realized.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A swamp red pseudo single-package flora water purifying agent is characterized in that: comprises the following components in percentage by weight: 0.5 percent of sodium acetate, 0.3 percent of ammonium chloride, 0.03 percent of magnesium sulfate, 0.07 percent of monopotassium phosphate, 0.04 percent of dipotassium phosphate, 0.06 percent of salt, 0.04 percent of beef extract, 0.02 percent of yeast extract, 0.5 percent of baking soda, 0.007 percent of riboflavin, 5 to 10 percent of bog brown monascus strain and the balance of pure water.
2. The water quality purifying agent for the swamp red pseudo single-package flora according to claim 1, which is characterized in that: the bog red false single-package strain is a third-generation strain which is obtained by sequentially culturing laboratory first-generation strains.
3. The water purifying agent for swamp red pseudo single-package flora according to claim 1 or 2, which is characterized in that: the finished product is reddish brown liquid, and the effective viable count is more than or equal to 5 multiplied by 108 CFU/ml.
4. The preparation method of the swamp red pseudo single-package flora water purifying agent according to claim 1 or 2, which is characterized in that: sterilizing and cleaning the production apparatus, weighing raw materials (not containing the rhodopseudomonas palustris strain) according to the required weight of each component, adding the raw materials into purified water, fully stirring to dissolve and uniformly mix the components, inoculating the rhodopseudomonas palustris strain with the required weight, stirring for at least 20 minutes, and finally filling and illuminating.
5. The preparation method of the swamp red pseudo single-package flora water purifying agent according to claim 4, which is characterized in that: the filling is carried out by adopting a transparent polyethylene barrel, and the illumination time is not less than 24 hours.
6. The preparation method of the swamp red pseudo single-package flora water purifying agent according to claim 4, which is characterized in that: when the rhodopseudomonas palustris strain is cultured, the raw materials of a culture medium comprise ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate, yeast extract and distilled water, wherein the addition amount of the ammonium chloride, the dipotassium hydrogen phosphate, the sodium bicarbonate and the yeast extract is 1.5g, 0.4g, 2g and 3g in sequence per 100mL of the culture medium, and the balance is the distilled water.
7. The preparation method of the swamp red pseudo single-package flora water purifying agent according to claim 6, which is characterized in that: when the enlarged culture of the rhodopseudomonas palustris is carried out, the inoculation amount of the rhodopseudomonas palustris is 20-25%.
8. The preparation method of the swamp red pseudo single-package flora water purifying agent according to claim 7, which is characterized in that: when the rhodopseudomonas palustris strain is cultured, the pH value of the culture medium is between 7 and 7.5.
9. The preparation method of the swamp red pseudo single-package flora water purifying agent according to claim 8, which is characterized in that: when the rhodopseudomonas palustris strain is cultured, the culture temperature is 30-35 ℃, the illumination intensity is 2000Lx, and the culture time is 48 hours.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20080145917A1 (en) * | 2003-05-14 | 2008-06-19 | Daniel Garcia | Method of Degrading Tbp Using a Photosynthetic Bacterial Strain |
| CN108913680A (en) * | 2018-08-08 | 2018-11-30 | 国家海洋局第三海洋研究所 | A kind of microorganism formulation and its application for cultivation water environment ammonia nitrogen degradation |
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- 2019-09-29 CN CN201910934814.5A patent/CN110627224A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080145917A1 (en) * | 2003-05-14 | 2008-06-19 | Daniel Garcia | Method of Degrading Tbp Using a Photosynthetic Bacterial Strain |
| CN108913680A (en) * | 2018-08-08 | 2018-11-30 | 国家海洋局第三海洋研究所 | A kind of microorganism formulation and its application for cultivation water environment ammonia nitrogen degradation |
Non-Patent Citations (1)
| Title |
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| 蔡慧农等: "沼泽红假单胞菌培养基的优化及降氨氮作用的研究", 《集美大学学报(自然科学版)》 * |
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