CN110623901A

CN110623901A - Freeze-dried powder for beauty treatment and production process thereof

Info

Publication number: CN110623901A
Application number: CN201911108751.4A
Authority: CN
Inventors: 不公告发明人
Original assignee: Changsha Kaize Engineering Design Co Ltd
Current assignee: Changsha Kaize Engineering Design Co Ltd
Priority date: 2019-11-13
Filing date: 2019-11-13
Publication date: 2019-12-31

Abstract

The invention provides a freeze-dried powder for beauty treatment and a production process thereof, wherein active peptide and sialic acid are added into a fresh corn-cactus mixed extracting solution, the mixture is uniformly mixed, the first freeze-drying is carried out to obtain an intermediate product, the first protection of the active peptide and the sialic acid is realized, then precooled cocoa bean-tremella mixed fermentation liquor is sprayed on the surface of the intermediate product, the second freeze-drying is carried out to realize the second protection of the active peptide and the sialic acid, the obtained freeze-dried powder product can be stored for a long time without lower temperature, and the problems of deterioration and the like can not occur for a period of time after being unsealed, thereby greatly improving the stability of the freeze-dried.

Description

Freeze-dried powder for beauty treatment and production process thereof

Technical Field

The invention relates to the technical field of beauty products, in particular to freeze-dried powder for beauty and a production process thereof.

Background

Human skin is bearing the injury such as ultraviolet ray, haze that come from the external world, moreover along with the continuous worsening of human living environment and the continuous acceleration of life rhythm, environmental pollution and life pressure etc. all can bring a lot of problems to human skin, for example: dry skin, color spots, pimples, wrinkles, sensitive skin, etc. In addition, as the demand for quality of life is greatly increased, the demand for beauty is also increasing. With the formation of the general health concept and the pursuit of higher quality of life, the development of the Chinese beauty industry is rapidly promoted in recent years, and the development rate is increased by more than 20% every year, so that the Chinese beauty industry is the third global market and has wide industrial prospect.

At present, many freeze-dried powder products for beauty treatment exist on the market, wherein freeze drying, namely freeze drying, also called sublimation drying, is a drying method for freezing a material solution to be dried into a solid in advance and then directly subliming to remove water from the frozen state without passing through a liquid state under the conditions of low temperature and low pressure. The freeze-dried powder is distinguished according to efficacy, and has whitening, repairing, acne removing, wrinkle resisting and the like. The freeze-dried powder is a biological component with high activity, is powdered by a freezing technology, well ensures the high activity of the original cosmetic components, achieves ultrahigh stability, is mixed with lysozyme, is quickly recovered and is convenient to use. High activity, ensures the nutrient components of the product, can be better absorbed by cells, thereby shortening and improving the skin cycle.

The freeze-dried powder has two using modes, one is used for injecting a microneedle, and the other is used for directly smearing. The micro-needle is a use mode of a beauty shop, but the operation is relatively complex for an ordinary client, injection is required to be carried out in the beauty shop, and the beauty cost is increased virtually. The direct smearing mode is undoubtedly more convenient and more popular with people.

At present, a plurality of freeze-dried powder products for beauty treatment exist in the market, the components of the freeze-dried powder products are various active polypeptides, the biggest defect is that the storage conditions are harsh, the freeze-dried powder products are usually stored at the temperature of minus 20 ℃ and below, and the storage and transportation difficulty is increased. Moreover, once the bag is unsealed, the residual freeze-dried powder can quickly deteriorate under the influence of air, various bad and even toxic substances are generated, and the freeze-dried powder can only be discarded, so that waste is caused.

Disclosure of Invention

The invention aims to provide freeze-dried powder for beauty treatment and a production process thereof, which aim to solve the technical problems of low storage temperature, easy deterioration after unsealing and the like.

In order to achieve the purpose, the invention provides a production process of freeze-dried powder for beauty treatment, which comprises the steps of adding active peptide and sialic acid into a fresh corn-cactus mixed extracting solution, uniformly mixing, carrying out primary freeze drying to obtain an intermediate product, spraying precooled cocoa bean-tremella mixed fermentation liquor on the surface of the intermediate product, carrying out secondary freeze drying, and packaging to obtain the freeze-dried powder for beauty treatment; the active peptide is a combination of 1 part of carnosine, 0.5-0.8 part of acetyl tetrapeptide-5, 0.1-0.2 part of bonito elastin peptide and 0.02-0.05 part of nisin in parts by weight.

Preferably, the mass ratio of the active peptide, the sialic acid, the fresh corn-cactus mixed extract and the cocoa bean-tremella mixed fermentation liquor is 1: 0.2-0.4: 6-8: 8 to 10.

Further preferably, the preparation method of the fresh corn-cactus mixed extracting solution comprises the following steps: adding 1 part of fresh corn kernels into 5-8 parts of water for injection by weight, crushing by a wet method, and then adopting CO₂Performing supercritical fluid extraction, separating and collecting volatile active ingredients and fat-soluble active ingredients, finally adding the collected extraction product into 3-4 parts of cactus juice, and performing ultrasonic oscillation treatment for 20-30 minutes to obtain the fresh corn-cactus mixed extracting solution.

More preferably, the cactus juice is a mucilage obtained by squeezing succulent stems of cactus.

Even more preferably, CO₂The extraction temperature of the supercritical fluid extraction is 35-45 ℃, the extraction pressure is 35-40 MPa, the extraction time is 2-3 hours, and CO is added₂The flow rate is 22-26L/h; the separation is carried out at the temperature of 35-45 ℃ and under the pressure of 5-8 MPa.

Further preferably, the preparation method of the cocoa bean-tremella mixed fermentation liquor comprises the following steps: cleaning 1 part of cocoa beans and 4-5 parts of tremella by weight, draining, transferring into a steam explosion tank, introducing nitrogen until the pressure in the steam explosion tank is 0.8-1 MPa, and carrying out explosion treatment for 8-15 minutes; and then, rapidly introducing steam until the pressure in the steam explosion tank is 2-2.5 MPa, carrying out steam explosion treatment for 1-2 minutes, inoculating lactobacillus according to the proportion of 1.5-2% of the inoculation amount, keeping the temperature at 35-37 ℃, and fermenting for 22-30 hours to obtain the cocoa bean-tremella mixed fermentation liquor.

Preferably, the specific method for blending is as follows: and (3) carrying out 30-40 MHz ultrasonic oscillation for 40-50 minutes.

Preferably, the sterilization treatment is performed before the first freeze-drying and the second freeze-drying, and the specific method comprises the following steps: sterile filtration through a 0.22 μm filter.

Preferably, the process conditions of the first freeze drying are as follows: freezing for 6-8 hours at the temperature of-20 to-25 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 30-35 ℃, and preserving heat for 3-5 hours.

Preferably, in an environment of-5 to-10 ℃, the intermediate product is uniformly spread to be 1 to 2mm, and then precooled cocoa bean-tremella mixed fermentation liquor is uniformly sprayed on the surface of the intermediate product, wherein the precooling temperature is-5 to-10 ℃.

Preferably, the process conditions of the second freeze drying are as follows: freezing for 6-8 hours at the temperature of minus 20-minus 25 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 0-5 ℃ at the heating rate of 8-10 ℃/hour, preserving the heat for 3-4 hours, then heating to 30-35 ℃ at the heating rate of 1-2 ℃/hour, and preserving the heat for 7-10 hours.

Preferably, the specific method of packaging is as follows: subpackaging with a penicillin bottle of 10mL specification, and sealing with a gland.

Preferably, the active peptide is obtained by directly mixing carnosine, acetyl tetrapeptide-5, bonito elastin peptide and nisin according to a formula ratio, and the carnosine, the acetyl tetrapeptide-5, the bonito elastin peptide and the nisin are all solid powder or crystals, have good water solubility, can be fully dispersed in a fresh corn-cactus mixed extracting solution in a subsequent step even if the mixing is not uniform, and cannot influence the product effect.

The freeze-dried powder for beauty treatment is obtained by the production process.

The invention has the following beneficial effects:

the preparation method comprises the steps of adding active peptide and sialic acid into a fresh corn-cactus mixed extracting solution, uniformly mixing, carrying out primary freeze drying to obtain an intermediate product, realizing primary protection on the active peptide and the sialic acid, spraying precooled cocoa bean-tremella mixed fermentation liquor on the surface of the intermediate product, carrying out secondary freeze drying, realizing secondary protection on the active peptide and the sialic acid, storing the obtained freeze-dried powder product for a long time without a low temperature, avoiding the problems of deterioration and the like for a period of time after unsealing, and greatly improving the stability of the freeze-dried powder product.

The active peptide is composed of carnosine, acetyl tetrapeptide-5, bonito elastin peptide and nisin, and the dosage of the four is reduced in sequence. Carnosine, a dipeptide consisting of beta-alanine and histidine, is capable of preventing skin aging and whitening skin, has antioxidant properties, and is a natural antioxidant; acetyl tetrapeptide-5 is a tetrapeptide, known as a moisturizing or hygroscopic agent, that helps to reduce ocular edema, improve skin elasticity and overall smoothness; the extraction source of the skipjack elastin peptide is the skipjack heart, the skipjack elastin peptide is composed of 2-20 amino acids, belongs to elastin peptide, and has the effects of promoting skin injury repair, blood vessel regeneration, promoting fibroblast proliferation and the like; the nisin is a small peptide consisting of 34 amino acids, and has strong inhibiting effect on gram-positive bacteria and the like. The invention uses carnosine, acetyl tetrapeptide-5, bonito elastin peptide and nisin with different molecular weights in a matching way, and the introduction of the relative macromolecular peptide is mainly to enrich the amino acid types and achieve better beautifying effect. Carnosine with small molecular weight accounts for the largest proportion, nisin with large molecular weight accounts for the smallest proportion, so that the skin of a human body can absorb small molecules more easily, and the absorption of the small molecules can assist the diffusion of macromolecules so as to be absorbed by the skin. Therefore, the amount of the peptide with large molecular weight should not be too large, which may result in skin failure to absorb the peptide.

The active peptide is matched with sialic acid for use, the sialic acid, also called N-acetyl neuraminic acid, is a derivative of the neuraminic acid, has the functions of protecting and stabilizing cells, has extremely strong negative charges, can enable cell membranes to have negative charges, and promotes the transfer of small peptides due to the existence of the sialic acid because the transfer of the small peptides depends on the positive charges (hydrogen ions, metal ions and the like) so as to be beneficial to skin absorption.

The fresh corn contains the phytosterol besides minerals and the like which are beneficial to the skin, has high permeability on the skin, can guide other components to permeate into the skin, can keep the moisture on the surface of the skin, promote the metabolism of the skin and inhibit the skin inflammation, and also has certain oxidation resistance. The cactus is rich in colloidal substances, and is helpful for forming a colloidal film on the skin surface to play a role in moisturizing; the cactus also contains phytosterol which can remove hydroxyl free radicals and superoxide anions, thereby being beneficial to accelerating the blocking effect of flavonoid compounds in the fresh corn on free radical chain reaction, and the fresh corn and the cactus synergistically play an anti-oxidation role. Therefore, the first important protection formed by the fresh corn-cactus mixed extract is to improve the oxidation resistance (improve the stability of the freeze-dried powder product) and guide the skin to absorb, which is beneficial to repairing and moisturizing the skin and achieving the beautifying effect.

The cocoa beans contain a large amount of antioxidant polyphenol, and the tremella contains amino acids, minerals and the like which are beneficial to the skin, and also contains a large amount of colloid components to form a structure similar to a protective film, and the film-shaped structure formed by the tremella is beneficial to uniform dispersion of polyphenol, so that the polyphenol becomes an isolation layer between the skin of a human body and the air, and the antioxidation of the polyphenol is fully exerted. Cocoa beans and tremella can degrade contained fat (easy rancidity) through fermentation treatment, meanwhile, macromolecular protein is degraded into micromolecular peptide or amino acid, the sialic acid can promote the micromolecular peptide or amino acid to be directly absorbed by skin, the amino acid types are further enriched on the basis of the active peptide, epidermal cells are jointly activated, nutrition is supplemented for the skin, fibroblast cells of the dermis layer are stimulated, collagen synthesis is accelerated, and the cosmetic effect of repairing and moisturizing is achieved. The significance of the secondary protection formed by the cocoa bean-tremella mixed fermentation liquor lies in further improving the oxidation resistance, namely further improving the stability of the freeze-dried powder product.

In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below.

Detailed Description

The following is a detailed description of embodiments of the invention, but the invention can be implemented in many different ways, as defined and covered by the claims.

The bonito elastin peptide is purchased from Shanxi Yikang dragon biotechnology limited company, and the raw material source of the bonito elastin peptide is imported from Japan.

Example 1:

a production process of lyophilized powder for skin care comprises adding active peptide and sialic acid into fresh semen Maydis-radix et caulis Opuntiae Dillenii mixed extractive solution, mixing, freeze drying to obtain intermediate product, spraying pre-cooled cacao bean-Tremella mixed fermentation broth onto the surface of the intermediate product, freeze drying for the second time, and packaging to obtain the lyophilized powder for skin care; the active peptide is a combination of 1 part of carnosine, 0.5 part of acetyl tetrapeptide-5, 0.2 part of bonito elastin peptide and 0.02 part of nisin in parts by weight.

The mass ratio of active peptide, sialic acid, fresh corn-cactus mixed extract and cocoa bean-tremella mixed fermentation liquor is 1: 0.4: 6: 10.

the preparation method of the fresh corn-cactus mixed extracting solution comprises the following steps: adding 1 part of fresh corn kernels into 5 parts of water for injection by weight, crushing by a wet method, and then adopting CO₂Performing supercritical fluid extraction, separating and collecting volatile effective components and fat-soluble effective components, adding the collected extraction product into 4 parts of cactus juice, and performing ultrasonic oscillation treatment for 20 minutes to obtain the fresh corn-cactus mixed extract. The cactus juice is a mucus obtained by squeezing succulent stem of cactus. CO 2₂The supercritical fluid extraction temperature is 45 deg.C, extraction pressure is 35MPa, extraction time is 3 hr, and CO is added₂The flow rate is 22L/h; the separation temperature is 45 ℃ and the pressure is 5 MPa.

The preparation method of the cocoa bean-tremella mixed fermentation liquor comprises the following steps: cleaning 1 part of cocoa beans and 4 parts of tremella, draining, transferring into a steam explosion tank, introducing nitrogen until the pressure in the steam explosion tank is 1MPa, and performing explosion treatment for 8 minutes; then, steam is rapidly introduced until the pressure in the steam explosion tank is 2.5MPa, after the steam explosion treatment is carried out for 1 minute, lactobacillus is inoculated according to the proportion of 2 percent of the inoculation amount, the temperature is kept at 35 ℃, and after the fermentation is carried out for 30 hours, the cocoa bean-tremella mixed fermentation liquor is obtained.

The concrete method for uniformly mixing comprises the following steps: 30MHz ultrasound was shaken for 50 minutes.

Before the first freeze drying and the second freeze drying, the sterilization treatment is carried out, and the specific method comprises the following steps: sterile filtration through a 0.22 μm filter.

The process conditions of the first freeze drying are as follows: freezing at-20 deg.C for 8 hr, vacuumizing to below 5Pa, maintaining the vacuum degree, heating to 30 deg.C, and holding for 5 hr.

In the environment of-5 ℃, the intermediate product is uniformly spread to 2mm, and then precooled cocoa bean-tremella mixed fermentation liquor is uniformly sprayed on the surface of the intermediate product, wherein the precooling temperature is-5 ℃.

The process conditions of the second freeze drying are as follows: freezing at-25 deg.C for 6 hr, vacuumizing to below 5Pa, maintaining the vacuum degree, heating to 0 deg.C at a rate of 10 deg.C/hr, holding for 4 hr, heating to 35 deg.C at a rate of 1 deg.C/hr, and holding for 7 hr.

Example 2:

a production process of lyophilized powder for skin care comprises adding active peptide and sialic acid into fresh semen Maydis-radix et caulis Opuntiae Dillenii mixed extractive solution, mixing, freeze drying to obtain intermediate product, spraying pre-cooled cacao bean-Tremella mixed fermentation broth onto the surface of the intermediate product, freeze drying for the second time, and packaging to obtain the lyophilized powder for skin care; the active peptide is a combination of 1 part of carnosine, 0.8 part of acetyl tetrapeptide-5, 0.1 part of bonito elastin peptide and 0.05 part of nisin in parts by weight.

The mass ratio of active peptide, sialic acid, fresh corn-cactus mixed extract and cocoa bean-tremella mixed fermentation liquor is 1: 0.2: 8: 8.

the preparation method of the fresh corn-cactus mixed extracting solution comprises the following steps: adding 1 part of fresh corn kernels into 8 parts of water for injection by weight, crushing by a wet method, and then adopting CO₂Extracting with supercritical fluid, separating and collecting volatile and liposoluble effective componentsAnd finally, adding the collected extraction product into 3 parts of cactus juice, and carrying out ultrasonic oscillation treatment for 30 minutes to obtain the fresh corn-cactus mixed extraction solution. The cactus juice is a mucus obtained by squeezing succulent stem of cactus. CO 2₂The supercritical fluid extraction temperature is 35 deg.C, extraction pressure is 40MPa, extraction time is 2 hr, and CO is added₂The flow rate is 26L/h; the separation temperature is 35 ℃ and the pressure is 8 MPa.

The preparation method of the cocoa bean-tremella mixed fermentation liquor comprises the following steps: cleaning 1 part of cocoa beans and 5 parts of tremella, draining, transferring into a steam explosion tank, introducing nitrogen until the pressure in the steam explosion tank is 0.8MPa, and carrying out explosion treatment for 15 minutes; then, steam is rapidly introduced until the pressure in the steam explosion tank is 2MPa, after the steam explosion treatment is carried out for 2 minutes, lactobacillus is inoculated according to the proportion of 1.5 percent of the inoculation amount, the temperature is kept at 37 ℃, and the cocoa bean-tremella mixed fermentation liquor is obtained after fermentation is carried out for 22 hours.

The concrete method for uniformly mixing comprises the following steps: 40MHz ultrasound was shaken for 40 minutes.

The process conditions of the first freeze drying are as follows: freezing at-25 deg.C for 6 hr, vacuumizing to below 5Pa, maintaining the vacuum degree, heating to 35 deg.C, and holding for 3 hr.

In the environment of-10 ℃, the intermediate product is uniformly spread to be 1mm, and then precooled cocoa bean-tremella mixed fermentation liquor is uniformly sprayed on the surface of the intermediate product, wherein the precooling temperature is-10 ℃.

The process conditions of the second freeze drying are as follows: freezing at-20 deg.C for 8 hr, vacuumizing to below 5Pa, maintaining the vacuum degree, heating to 5 deg.C at a rate of 8 deg.C/hr, holding for 3 hr, heating to 30 deg.C at a rate of 2 deg.C/hr, and holding for 10 hr.

Example 3:

a production process of lyophilized powder for skin care comprises adding active peptide and sialic acid into fresh semen Maydis-radix et caulis Opuntiae Dillenii mixed extractive solution, mixing, freeze drying to obtain intermediate product, spraying pre-cooled cacao bean-Tremella mixed fermentation broth onto the surface of the intermediate product, freeze drying for the second time, and packaging to obtain the lyophilized powder for skin care; the active peptide is a combination of 1 part of carnosine, 0.6 part of acetyl tetrapeptide-5, 0.15 part of bonito elastin peptide and 0.04 part of nisin in parts by weight.

The mass ratio of active peptide, sialic acid, fresh corn-cactus mixed extract and cocoa bean-tremella mixed fermentation liquor is 1: 0.3: 7: 9.

the preparation method of the fresh corn-cactus mixed extracting solution comprises the following steps: adding 1 part of fresh corn kernels into 6 parts of water for injection by weight, crushing by a wet method, and then adopting CO₂Performing supercritical fluid extraction, separating and collecting volatile effective components and fat-soluble effective components, adding the collected extraction product into 3.5 parts of cactus juice, and performing ultrasonic oscillation treatment for 25 minutes to obtain the fresh corn-cactus mixed extract. The cactus juice is a mucus obtained by squeezing succulent stem of cactus. CO 2₂The extraction temperature of the supercritical fluid extraction is 40 ℃, the extraction pressure is 38MPa, the extraction time is 2.5 hours, and CO is added₂The flow rate is 25L/h; the separation is carried out at 40 ℃ and under 6 MPa.

The preparation method of the cocoa bean-tremella mixed fermentation liquor comprises the following steps: cleaning 1 part of cocoa beans and 4 parts of tremella, draining, transferring into a steam explosion tank, introducing nitrogen until the pressure in the steam explosion tank is 0.9MPa, and carrying out explosion treatment for 12 minutes; then, steam is rapidly introduced until the pressure in the steam explosion tank is 2.2MPa, after the steam explosion treatment is carried out for 1 minute, lactobacillus is inoculated according to the proportion of 1.8 percent of the inoculation amount, the temperature is kept at 36 ℃, and after the fermentation is carried out for 28 hours, the cocoa bean-tremella mixed fermentation liquor is obtained.

The concrete method for uniformly mixing comprises the following steps: 35MHz ultrasound was shaken for 45 minutes.

The process conditions of the first freeze drying are as follows: freezing at-22 deg.C for 7 hr, vacuumizing to below 5Pa, maintaining the vacuum degree, heating to 32 deg.C, and holding for 4 hr.

In an environment of-8 ℃, uniformly spreading the intermediate product to 1.5mm, and then uniformly spraying precooled cocoa bean-tremella mixed fermentation liquor on the surface of the intermediate product, wherein the precooling temperature is-8 ℃.

The process conditions of the second freeze drying are as follows: freezing at-22 deg.C for 7 hr, vacuumizing to below 5Pa, maintaining the vacuum degree, heating to 3 deg.C at a rate of 9 deg.C/hr, holding for 4 hr, heating to 32 deg.C at a rate of 1.5 deg.C/hr, and holding for 8 hr.

Comparative example 1

The procedure of example 1 was followed except that the fresh corn-cactus mixed extract and the corresponding first freeze-drying were omitted.

Comparative example 2

The cocoa bean-tremella mixed fermentation broth and the corresponding second freeze-drying were omitted, as in example 1.

Comparative example 3

The nisin was omitted and the remainder was the same as in example 1.

Comparative example 4

The procedure of example 1 was repeated except that the skipjack elastin peptide was omitted.

Test examples

1. Stability survey

The stability of the carnosine content is inspected, and the freeze-dried powder obtained in the examples 1-3 and the comparative examples 1 and 2 is placed at 4 ℃ for 6 months and 24 months to inspect the carnosine content; and inspecting the carnosine content of the freeze-dried powder product after being unsealed for 1 hour and 12 hours (using a carnosine detection kit, detecting a sample as an aqueous solution, namely adding the freeze-dried powder into injection water with the weight of 3 times of that of the injection water, oscillating up and down and mixing uniformly, and then detecting, and directly comparing the carnosine content in the aqueous solution). The results are shown in Table 1.

TABLE 1 stability test results

As can be seen from Table 1, the lyophilized powder products obtained in examples 1-3 can be stored for a long time (more than 24 months at 4 ℃) without a low temperature, and the problems of deterioration and the like do not occur for a while after being unsealed, so that the stability of the lyophilized powder products is greatly improved. In the comparative example 1, the fresh corn-cactus mixed extract and the corresponding first freeze drying are omitted, in the comparative example 2, the cocoa bean-tremella mixed fermentation liquor and the corresponding second freeze drying are omitted, and the stability of the freeze-dried powder product is obviously deteriorated.

2. Examination of cosmetic effects

Healthy SD rats (purchased from Kyork Biotechnology (Shanghai) Co., Ltd.) were divided into 5 groups of half male and female, each group was prepared by preparing skin on the back of the rat for 24 hours, gently and repeatedly applying glycolic acid with a mass concentration of 50% to the depilated area at 2 cm. times.2 cm for 10 minutes, and then removing the excess liquid with a cotton ball, thus obtaining a skin cutin exfoliation injury model. Then respectively smearing an aqueous solution (obtained by adding the freeze-dried powder into injection water with the weight of 3 times that of the freeze-dried powder and uniformly mixing the freeze-dried powder with the water for injection) prepared by the freeze-dried powder obtained in the embodiments 1-3 or the comparative examples 3 and 4 on one side of the skin injury of the rat, smearing physiological saline on the other side of the skin injury of the rat twice a day for 15 days continuously, observing the skin of the animal and the whole animal in the experimental process, judging whether irritation and adverse reaction occur after the skin injury is used, and detecting the water content of the injury skin in 15 days. The moisture content of the skin was measured using a moisture tester. The results are shown in Table 2.

TABLE 2 cosmetic Effect examination

	Average moisture content of skin (%)
		Example 1	32.33
Example 1 control side	11.08
		Example 2	32.78
Example 2 control side	11.23
		Example 3	33.53
Example 3 control side	11.16
		Comparative example 3	20.01
Comparative example 3 control side	11.22
		Comparative example 4	19.68
Comparative example 4 control side	11.21

After skin injury, the skin of a rat with the freeze-dried powder products of the embodiments 1-3 can be obviously improved, after the freeze-dried powder products are smeared for 15 days, the scars of the administration side are obviously less than those of the control side, and the water content of the administration side is obviously higher than that of the control side when the freeze-dried powder products are detected out from the table 2. In comparative example 3, nisin and skipjack elastin peptide are omitted in comparative example 4, and the repairing moisturizing effect on exfoliating damaged skin is certain, but compared with examples 1-3, the repairing improvement condition is obviously poor, namely the cosmetic effect of the lyophilized powder products in comparative example 3 and comparative example 4 is obviously poor.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A production process of freeze-dried powder for beauty is characterized in that active peptide and sialic acid are added into a fresh corn-cactus mixed extracting solution, evenly mixed, freeze-dried for the first time to obtain an intermediate product, then precooled cocoa bean-tremella mixed fermentation liquor is sprayed on the surface of the intermediate product, and freeze-dried for the second time and packaged to obtain the freeze-dried powder for beauty; the active peptide is a combination of 1 part of carnosine, 0.5-0.8 part of acetyl tetrapeptide-5, 0.1-0.2 part of bonito elastin peptide and 0.02-0.05 part of nisin in parts by weight.

2. The production process according to claim 1, wherein the mass ratio of the active peptide, the sialic acid, the fresh corn-cactus mixed extract and the cocoa bean-tremella mixed fermentation liquor is 1: 0.2-0.4: 6-8: 8 to 10.

3. The production process according to claim 2, wherein the preparation method of the fresh corn-cactus mixed extract is as follows: adding 1 part of fresh corn kernels into 5-8 parts of water for injection by weight, crushing by a wet method, and then adopting CO₂Performing supercritical fluid extraction, separating and collecting volatile active ingredients and fat-soluble active ingredients, finally adding the collected extraction product into 3-4 parts of cactus juice, and performing ultrasonic oscillation treatment for 20-30 minutes to obtain the fresh corn-cactus mixed extracting solution.

4. The production process according to claim 3, wherein CO is₂The extraction temperature of the supercritical fluid extraction is 35-45 ℃, the extraction pressure is 35-40 MPa, the extraction time is 2-3 hours, and CO is added₂The flow rate is 22-26L/h; what is needed isThe separation is carried out at the temperature of 35-45 ℃ and under the pressure of 5-8 MPa.

5. The production process according to claim 2, wherein the cocoa bean-tremella mixed fermentation broth is prepared by the following method: cleaning 1 part of cocoa beans and 4-5 parts of tremella by weight, draining, transferring into a steam explosion tank, introducing nitrogen until the pressure in the steam explosion tank is 0.8-1 MPa, and carrying out explosion treatment for 8-15 minutes; and then, rapidly introducing steam until the pressure in the steam explosion tank is 2-2.5 MPa, carrying out steam explosion treatment for 1-2 minutes, inoculating lactobacillus according to the proportion of 1.5-2% of the inoculation amount, keeping the temperature at 35-37 ℃, and fermenting for 22-30 hours to obtain the cocoa bean-tremella mixed fermentation liquor.

6. The production process of claim 1, wherein the specific method of blending is: and (3) carrying out 30-40 MHz ultrasonic oscillation for 40-50 minutes.

7. The production process according to claim 1, wherein the first freeze-drying process conditions are as follows: freezing for 6-8 hours at the temperature of-20 to-25 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 30-35 ℃, and preserving heat for 3-5 hours.

8. The production process according to claim 1, wherein the intermediate product is spread uniformly to 1-2 mm in an environment of-5 to-10 ℃, and then precooled cocoa bean-tremella mixed fermentation liquor is sprayed uniformly on the surface of the intermediate product, wherein the precooling temperature is-5 to-10 ℃.

9. The production process according to claim 1, wherein the process conditions of the second freeze-drying are as follows: freezing for 6-8 hours at the temperature of minus 20-minus 25 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 0-5 ℃ at the heating rate of 8-10 ℃/hour, preserving the heat for 3-4 hours, then heating to 30-35 ℃ at the heating rate of 1-2 ℃/hour, and preserving the heat for 7-10 hours.

10. A freeze-dried powder for beauty treatment obtained by the production process of any one of claims 1 to 9.