CN110583623A - Medical low-temperature cryopreservation method - Google Patents
Medical low-temperature cryopreservation method Download PDFInfo
- Publication number
- CN110583623A CN110583623A CN201910821948.6A CN201910821948A CN110583623A CN 110583623 A CN110583623 A CN 110583623A CN 201910821948 A CN201910821948 A CN 201910821948A CN 110583623 A CN110583623 A CN 110583623A
- Authority
- CN
- China
- Prior art keywords
- sample
- vertical adjusting
- lifting plate
- freezing
- box door
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 21
- 238000007710 freezing Methods 0.000 claims abstract description 56
- 230000008014 freezing Effects 0.000 claims abstract description 55
- 238000005192 partition Methods 0.000 claims abstract description 24
- 238000004321 preservation Methods 0.000 claims description 24
- 239000002577 cryoprotective agent Substances 0.000 claims description 11
- 238000003825 pressing Methods 0.000 claims description 7
- 238000005057 refrigeration Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 5
- 239000000725 suspension Substances 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 103
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
Landscapes
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a medical low-temperature cryopreservation method, which can obviously save the time for loading and taking samples and avoid the influence on the cryopreservation effect of other samples in a box due to long-time operation. When a sample is stored, the lifting plate is lifted to the highest point in advance, then the sample is placed above the partition plate or the tray, the box door is closed randomly, and then the lifting plates are pressed on the upper part of the sample by screwing the vertical adjusting bolt to evenly flatten the sample; when a sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed, so that the box door can be quickly closed when the sample is taken and put. Keep vibrating motor work at freezing in-process, vibrating motor drives the vibration of freezing storage box, and after freezing link, vibrating motor stop work makes the sample be in suspension state all the time, can effectively avoid sample direct contact bag body wall.
Description
Technical Field
The invention relates to the technical field of medical low-temperature preservation, in particular to a medical low-temperature cryopreservation method.
Background
Cryopreservation refers to a process of freezing biological tissues in a solution added with a cryoprotectant to a certain low temperature and preserving the biological tissues for a long time, and has wide application and requirements in biological research, medical application and other aspects. The freezing process is generally that the biological sample containing the cryoprotectant is frozen to-80 ℃ in a low-temperature refrigerator, then is frozen to-196 ℃ in liquid nitrogen and is stored in the liquid nitrogen for a long time. At present, most of containers for low-temperature cryopreservation are cryopreservation box bodies or tank bodies with one open ends, sample bags are stacked in the cryopreservation box bodies or tank bodies, so that the sample bags are stacked out of order, and can only be sequentially lifted and turned when needing to be taken out; due to the disordered stacking of the samples, the thickness of each part of the bag body is uneven when the bag body is kept still, the cooling speed of the part with thin thickness is high, the cooling speed of the part with thick thickness is low, and the cooling efficiency is inconsistent; for larger-sized biological tissues, such as cell masses, tissues and the like, the sample is easy to directly contact the wall of the bag body in a standing state and is not protected by the cryoprotectant any more, so that the sample is damaged.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a medical low-temperature cryopreservation method so as to save the time for loading and taking a sample bag and avoid the influence on the cryopreservation effect of other samples in a box due to long-time operation.
The purpose of the invention is realized by the following technical scheme:
1. a medical low-temperature cryopreservation method comprises the following steps:
s1, a base of the freezing preservation box body, a supporting spring, a vibrating motor and a freezing preservation box body with a front opening are arranged, at least one partition board arranged along the horizontal direction is arranged in the freezing preservation box body, the partition board divides the inner space of the freezing preservation box body into a plurality of containing cavities which are sequentially arranged along the vertical direction, each containing cavity is internally provided with a lifting plate which is horizontally arranged, the left side and the right side of the top of the freezing preservation box body are respectively provided with a row of vertical adjusting bolts which are sequentially arranged along the front-back direction, the number of each row of vertical adjusting bolts is the same as that of the lifting plate, each row of vertical adjusting bolts are respectively corresponding to the lifting plates one by one, a top plate of the freezing preservation box body is provided with threaded holes which are matched with each vertical adjusting bolt, each vertical adjusting bolt is arranged in the corresponding threaded hole in a matched manner through threads, and the lower end of, the refrigerator comprises a refrigeration storage box body, a refrigeration storage box body base, a plurality of support springs, a vibration motor, a lifting plate, a baffle plate, a box door opening;
s2, screwing a vertical adjusting bolt corresponding to the lifting plate in the containing cavity in which the sample is not stored, lifting the lifting plate in the containing cavity in which the sample is not stored to the highest point, then opening the box door, placing the sample above the partition plate or the tray, and closing the box door;
s3, screwing the vertical adjusting bolts corresponding to the lifting plates placed in the containing cavity of the sample in the step S2, and pressing the lifting plates on the upper part of the sample to evenly flatten the sample;
s4, keeping the vibration motor working in the freezing process, driving the freezing storage box body to vibrate by the vibration motor, and stopping the vibration motor after the freezing link is finished;
s5, when the sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed immediately.
The invention can obviously save the time for loading and taking the samples and avoid the influence on the freezing and storing effect of other samples in the box due to long-time operation. When a sample is stored, the lifting plate is lifted to the highest point in advance, then the sample is placed above the partition plate or the tray, the box door is closed randomly, and then the lifting plates are pressed on the upper part of the sample by screwing the vertical adjusting bolt to evenly flatten the sample; when a sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed, so that the box door can be quickly closed when the sample is taken and put.
Preferably, the method further comprises the step of leading out the sample through the rotation of the roller.
Preferably, the method further comprises the step of taking out the sample by sliding the tray along the sliding rail.
Preferably, each containing cavity is provided with a sample, and the sample comprises a bag body, a cryoprotectant filled in the bag body and biological tissues immersed in the cryoprotectant.
Preferably, the bottom of each accommodating cavity is provided with a sliding rail extending along the front-back direction, and a tray for accommodating samples is slidably mounted on the sliding rail.
The invention has the following advantages:
the invention can obviously save the time for loading and taking the samples and avoid the influence on the freezing and storing effect of other samples in the box due to long-time operation. When a sample is stored, the lifting plate is lifted to the highest point in advance, then the sample is placed above the partition plate or the tray, the box door is closed randomly, and then the lifting plates are pressed on the upper part of the sample by screwing the vertical adjusting bolt to evenly flatten the sample; when a sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed, so that the box door can be quickly closed when the sample is taken and put.
Keep vibrating motor work at freezing in-process, vibrating motor drives the vibration of freezing storage box, and after freezing link, vibrating motor stop work makes the sample be in suspension state all the time, can effectively avoid sample direct contact bag body wall.
The height of the lifting plate of each accommodating cavity is independently adjusted, so that the lifting plate can be suitable for samples of different specifications.
Drawings
Fig. 1 is a main sectional structural view of the lifter plate of the present invention at the highest point.
FIG. 2 is a schematic view of the main cross-sectional structure of the lifting plate of the present invention when the lifting plate presses a sample.
FIG. 3 is a schematic side sectional view of the lifter plate of the present invention in a position for pressing a sample.
Fig. 4 is a main sectional structural view of the present invention when a tray structure is provided.
1-base of freezing preservation box, 2-supporting spring, 3-vibration motor, 41-freezing preservation box, 42-partition board, 43-lifting board, 44-vertical adjusting bolt, 45-box door, 46-convex edge, 47-roller, 48-slide rail and 49-tray.
Detailed Description
The invention is further described with reference to the accompanying drawings in which:
as shown in fig. 1, 2 and 3, a medical cryogenic cryopreservation method is used for a cryogenic cryopreservation device,
s2, a base 1 of the freezing preservation box body, a supporting spring 2, a vibrating motor 3 and a freezing preservation box body 41 with a front opening are arranged, at least one partition plate 42 arranged along the horizontal direction is arranged in the freezing preservation box body 41, the partition plate 42 divides the inner space of the freezing preservation box body 41 into a plurality of containing cavities which are sequentially arranged along the vertical direction, a lifting plate 43 arranged horizontally is arranged in each containing cavity, a row of vertical adjusting bolts 44 sequentially arranged along the front-back direction is arranged on the left side and the right side of the top of the freezing preservation box body 41, the number of each row of vertical adjusting bolts 44 is the same as that of the lifting plate 43, each row of vertical adjusting bolts 44 corresponds to the lifting plate 43 one by one, threaded holes matched with each vertical adjusting bolt 44 are arranged on the top plate of the freezing preservation box body 41, each vertical adjusting bolt 44 is arranged in the corresponding threaded hole in a threaded fit way, the lower end of each vertical adjusting bolt 44 is rotatably connected with the corresponding lifting plate 43, except the vertical adjusting bolt 44 connected with the lifting plate 43 positioned at the uppermost part, the other vertical adjusting bolts 44 pass through the lifting plate 43 and the partition plate 42 positioned above the corresponding lifting plate 43, the lifting plate 43 and the partition plate 42 are provided with abdicating holes for the vertical adjusting bolts 44 to pass through, the lifting plate 43 connected with the vertical adjusting bolts 44 can be driven to lift in the vertical direction in the accommodating cavity by screwing the vertical adjusting bolts 44, the front end of the freezing and preserving box 41 is provided with a box door 45 with a sealed front opening, the freezing and preserving box 41 is arranged on the freezing and preserving box base 1 through a plurality of supporting springs 2, and the vibration motor 3 is fixedly arranged on the freezing and preserving box 41.
S2, screwing the vertical adjusting bolt 44 corresponding to the lifting plate 43 in the containing cavity in which the sample is not stored, lifting the lifting plate 43 in the containing cavity in which the sample is not stored to the highest point, then opening the box door 45, placing the sample above the partition plate 42 or the tray 49, and closing the box door 45;
s3, screwing the vertical adjusting bolts 44 corresponding to the lifting plates 43 placed in the accommodating cavity of the sample in the step S2, and pressing the lifting plates 43 on the upper part of the sample to evenly flatten the sample;
s4, keeping the vibration motor 3 working in the freezing process, driving the freezing preservation box body 41 to vibrate by the vibration motor 3, and stopping the vibration motor 3 after the freezing link is finished;
s5, when the sample needs to be taken out, the vertical adjusting bolt 44 corresponding to the lifting plate 43 covering the sample is firstly screwed, so that the lifting plate 43 is lifted to the highest point to be separated from the sample, then the box door 45 is opened to take out the sample, and the box door 45 is closed immediately.
Preferably, the two vertical adjusting bolts 44 connected to the same lifting plate 43 are arranged substantially symmetrically about the center of the lifting plate 43, and the central symmetry need not be precise, but only substantially symmetrical, so as to avoid the lifting plate 43 from being stressed unevenly and causing jamming, and this is also a preferred design and is not necessary. In fact, the lifting driving force of the connecting plate is not large, the connecting plate can work smoothly even if the connecting plate is clamped, and the structure is only an optimized design.
A sample is placed in each containing cavity and comprises a bag body, a cryoprotectant filled in the bag body and biological tissues immersed in the cryoprotectant.
Preferably, the partition plate 42 is provided with a ridge 46 on both left and right sides, and the ridge 46 is located inside the vertical adjusting bolt 44 for preventing the sample on the partition plate 42 from contacting the vertical adjusting bolt 44.
Preferably, as shown in fig. 1, the bottom of each accommodating chamber is provided with a roller 47 having an axis arranged in a horizontal direction and perpendicular to the front-rear direction. The roller 47 serves to reduce the contact area of the sample with the partition 42 and take out the sample by the rotation of the roller 47. The sample is prevented from being damaged due to excessive contact and friction between the sample and the partition plate 42, and the sample is protected.
Preferably, as shown in fig. 4, the bottom of each accommodating cavity is provided with a slide rail 48 extending in the front-back direction, and a tray 49 for accommodating samples is slidably mounted on the slide rail 48.
When the sample pressing device is used, the vertical adjusting bolts 44 are screwed, as shown in fig. 1, the lifting plates 43 in the containing cavities are lifted to the highest point, then the samples are placed above the partition plate 42 or the tray 49, the box door 45 is closed, as shown in fig. 2 and 3, the vertical adjusting bolts 44 are screwed to press the lifting plates 43 on the upper parts of the samples so as to evenly flatten the samples;
the vibration motor 3 is kept working in the freezing process, the vibration motor 3 drives the freezing preservation box body 41 to vibrate, and after the freezing link is finished, the vibration motor 3 stops working.
When the sample needs to be taken out, the vertical adjusting bolt 44 corresponding to the lifting plate 43 covering the sample is firstly screwed, so that the lifting plate 43 is lifted to the highest point to be separated from the sample, then the box door 45 is opened to take out the sample, and then the box door 45 is closed.
Preferably, the door 45 is a transparent door 45 made of a transparent material. So that the accommodating cavity in which each sample is positioned can be seen without opening the door 45. Before the door 45 is opened, the corresponding vertical adjusting bolt 44 is operated to lift the lifting plate 43 corresponding to the upper part of the sample, so that the sample can be taken out quickly after the door 45 is opened, and the door 45 is closed quickly.
A medical cryo-preservation comprising the steps of: screwing the vertical adjusting bolt 44 corresponding to the lifting plate 43 in the containing cavity in which the sample is not stored, lifting the lifting plate 43 in the containing cavity in which the sample is not stored to the highest point, then opening the box door 45, placing the sample above the partition plate 42 or the tray 49, and closing the box door 45; screwing up vertical adjusting bolts 44 corresponding to the lifting plates 43 placed in the accommodating cavities of the samples in the step, and pressing the lifting plates 43 on the upper parts of the samples to uniformly flatten the samples; the vibration motor 3 is kept working in the freezing process, the vibration motor 3 drives the freezing preservation box body 41 to vibrate, and after the freezing link is finished, the vibration motor 3 stops working; when the sample needs to be taken out, the vertical adjusting bolt 44 corresponding to the lifting plate 43 covering the sample is firstly screwed, so that the lifting plate 43 is lifted to the highest point to be separated from the sample, then the box door 45 is opened to take out the sample, and then the box door 45 is closed.
Referring to fig. 1-4, a medical cryopreservation method further includes the step of deriving the sample by rotation of the roller 47. Further comprising the step of taking out the sample by sliding the tray 49 along the slide rail 48.
Two vertical adjusting bolts connected with the same lifting plate are arranged approximately symmetrically about the center of the lifting plate, the central symmetry does not need to be very accurate, only the approximately central symmetry is needed, so that the phenomenon that the lifting plate is stressed unevenly and clamped is avoided, and the design is also preferred and not necessary. In fact, the lifting driving force of the connecting plate is not large, the connecting plate can work smoothly even if the connecting plate is clamped, and the structure is only an optimized design. A sample is placed in each containing cavity and comprises a bag body, a cryoprotectant filled in the bag body and biological tissues immersed in the cryoprotectant. The bottom of each accommodating cavity is provided with a roller with an axis arranged along the horizontal direction and perpendicular to the front and back directions. The roller is used for reducing the contact area of the sample and the clapboard, and the sample is taken out through the rotation of the roller. The sample damage caused by excessive contact and friction between the sample and the partition plate is avoided, and the sample is protected. When the sample pressing device is used, the vertical adjusting bolts are screwed, the lifting plates in the containing cavities are lifted to the highest point, then the sample is placed above the partition plate or the tray, the box door is closed, and the lifting plates are pressed on the upper part of the sample to evenly flatten the sample by screwing the vertical adjusting bolts;
the vibration motor is kept working in the freezing process, the vibration motor drives the freezing storage box body to vibrate, and the vibration motor stops working after the freezing link is finished. When the sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed immediately.
The box door is a transparent box door made of transparent materials. Therefore, when the box door is not opened, the containing cavity in which each sample is located can be seen, the lifting plate corresponding to the upper part of the sample is lifted by operating the corresponding vertical adjusting bolt, the sample can be quickly taken out after the box door is opened, and the box door is quickly closed before the box door is opened.
The invention can obviously save the time for loading and taking the samples and avoid the influence on the freezing and storing effect of other samples in the box due to long-time operation. When a sample is stored, the lifting plate is lifted to the highest point in advance, then the sample is placed above the partition plate or the tray, the box door is closed randomly, and then the lifting plates are pressed on the upper part of the sample by screwing the vertical adjusting bolt to evenly flatten the sample; when a sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed, so that the box door can be quickly closed when the sample is taken and put. Keep vibrating motor work at freezing in-process, vibrating motor drives the vibration of freezing storage box, and after freezing link, vibrating motor stop work makes the sample be in suspension state all the time, can effectively avoid sample direct contact bag body wall. The height of the lifting plate of each accommodating cavity is independently adjusted, so that the lifting plate can be suitable for samples of different specifications.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (5)
1. A medical low-temperature cryopreservation method is characterized by comprising the following steps:
s1, a base of the freezing preservation box body, a supporting spring, a vibrating motor and a freezing preservation box body with a front opening are arranged, at least one partition board arranged along the horizontal direction is arranged in the freezing preservation box body, the partition board divides the inner space of the freezing preservation box body into a plurality of containing cavities which are sequentially arranged along the vertical direction, each containing cavity is internally provided with a lifting plate which is horizontally arranged, the left side and the right side of the top of the freezing preservation box body are respectively provided with a row of vertical adjusting bolts which are sequentially arranged along the front-back direction, the number of each row of vertical adjusting bolts is the same as that of the lifting plate, each row of vertical adjusting bolts are respectively corresponding to the lifting plates one by one, a top plate of the freezing preservation box body is provided with threaded holes which are matched with each vertical adjusting bolt, each vertical adjusting bolt is arranged in the corresponding threaded hole in a matched manner through threads, and the lower end of, the refrigerator comprises a refrigeration storage box body, a refrigeration storage box body base, a plurality of support springs, a vibration motor, a lifting plate, a baffle plate, a box door opening;
s2, screwing a vertical adjusting bolt corresponding to the lifting plate in the containing cavity in which the sample is not stored, lifting the lifting plate in the containing cavity in which the sample is not stored to the highest point, then opening the box door, placing the sample above the partition plate or the tray, and closing the box door;
s3, screwing the vertical adjusting bolts corresponding to the lifting plates placed in the containing cavity of the sample in the step S2, and pressing the lifting plates on the upper part of the sample to evenly flatten the sample;
s4, keeping the vibration motor working in the freezing process, driving the freezing storage box body to vibrate by the vibration motor, and stopping the vibration motor after the freezing link is finished;
s5, when the sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed immediately.
2. The medical cryopreservation method of claim 1, wherein: the method also comprises the step of leading out the sample through the rotation of the roller.
3. The medical cryopreservation method of claim 1, wherein: the method also comprises the step of taking out the sample by sliding the tray along the sliding rail.
4. The medical cryopreservation method of claim 1, wherein: a sample is placed in each containing cavity and comprises a bag body, a cryoprotectant filled in the bag body and biological tissues immersed in the cryoprotectant.
5. The medical cryopreservation method of claim 1, wherein: the bottom of every holding chamber is provided with the slide rail that extends along the fore-and-aft direction, and slidable mounting has the tray that is used for holding the sample on the slide rail.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910821948.6A CN110583623B (en) | 2019-09-02 | 2019-09-02 | Medical low-temperature cryopreservation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910821948.6A CN110583623B (en) | 2019-09-02 | 2019-09-02 | Medical low-temperature cryopreservation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110583623A true CN110583623A (en) | 2019-12-20 |
| CN110583623B CN110583623B (en) | 2020-11-27 |
Family
ID=68856856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910821948.6A Expired - Fee Related CN110583623B (en) | 2019-09-02 | 2019-09-02 | Medical low-temperature cryopreservation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110583623B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142413A (en) * | 2018-01-06 | 2018-06-12 | 谷昌德 | Freezing storage device |
| CN208395192U (en) * | 2018-05-14 | 2019-01-18 | 温州冲亚电子科技有限公司 | A kind of refrigerating box for bioengineering |
| CN208639453U (en) * | 2018-07-19 | 2019-03-26 | 卡替（上海）生物技术股份有限公司 | The safe storage device of stem cell |
| CN209209325U (en) * | 2018-11-06 | 2019-08-06 | 贵阳中医学院 | A kind of Saving specimen device for Blood laboratory research |
-
2019
- 2019-09-02 CN CN201910821948.6A patent/CN110583623B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142413A (en) * | 2018-01-06 | 2018-06-12 | 谷昌德 | Freezing storage device |
| CN208395192U (en) * | 2018-05-14 | 2019-01-18 | 温州冲亚电子科技有限公司 | A kind of refrigerating box for bioengineering |
| CN208639453U (en) * | 2018-07-19 | 2019-03-26 | 卡替（上海）生物技术股份有限公司 | The safe storage device of stem cell |
| CN209209325U (en) * | 2018-11-06 | 2019-08-06 | 贵阳中医学院 | A kind of Saving specimen device for Blood laboratory research |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110583623B (en) | 2020-11-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11946684B2 (en) | Spirally ascending/descending cryogenic storage apparatus | |
| CN107972999B (en) | Freezing box storage tank | |
| CN110589190B (en) | Medical low-temperature cryopreservation equipment | |
| CN110583623B (en) | Medical low-temperature cryopreservation method | |
| CN110817062A (en) | Sealed storage box capable of storing two kinds of tea | |
| CN109094952A (en) | A kind of stem cell and biologically active agents Special vehicle-mounted warehousing and transportation facilities | |
| CN111903661B (en) | Liquid nitrogen storage device | |
| CN212221116U (en) | Food transportation is with fresh-keeping device | |
| CN219340566U (en) | Feeding and discharging sealing mechanism for biological sample low-temperature storage mechanism | |
| CN214525320U (en) | Blood storage box convenient for cold storage and transportation | |
| KR200408917Y1 (en) | A Portable Type Cold Storage Barrel | |
| CN214650440U (en) | Automatic access gripper device for liquid nitrogen biological container | |
| CN111572971B (en) | Device for preserving umbilical cord blood stem cells | |
| CN220431018U (en) | Fresh delivery fresh-keeping case | |
| CN221139206U (en) | Food detection sample storage device | |
| CN213324378U (en) | Warehouse is with freezing vegetables storage device | |
| CN220062257U (en) | Ultralow temperature freezing preservation box | |
| CN218303223U (en) | Preparation of quick-freeze bright kernel of corn is with low energy consumption frozen machine | |
| CN109673625A (en) | Low-temperature environment small-unit biological sample storing and taking device | |
| CN218173064U (en) | Portable plant tissue sample refrigeration box | |
| CN217391167U (en) | Portable multifunctional medicine box | |
| CN213639439U (en) | Cell cryopreservation kit | |
| CN214932363U (en) | Reproductive sample cryopreserving device | |
| CN216612231U (en) | Sperm freezes to deposit transportation transfer storage's smart pole foam case of biotechnology | |
| CN219479037U (en) | Cell freezing storage cabinet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Hui Inventor after: Zhao Juan Inventor after: Xu Peiyan Inventor after: Li Yongjian Inventor before: Li Yongjian |
|
| CB03 | Change of inventor or designer information | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20201109 Address after: 510800 Xinhua 48, Xinhua Road, Huadu District, Guangdong, Guangzhou Applicant after: Li Hui Address before: 643214 group 4, Zhoujia village, Gufo Town, Fushun County, Zigong City, Sichuan Province Applicant before: Li Yongjian |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210202 Address after: No.48 Xinhua Road, Xinhua Street, Huadu District, Guangzhou, Guangdong 510800 Patentee after: HUADU DISTRICT PEOPLE'S HOSPITAL OF GUANGZHOU Address before: No.48 Xinhua Road, Xinhua Town, Huadu District, Guangzhou, Guangdong 510800 Patentee before: Li Hui |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201127 Termination date: 20210902 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |