CN110551712B

CN110551712B - Nucleic acid extraction machine table with gun head positioning device

Info

Publication number: CN110551712B
Application number: CN201810565425.5A
Authority: CN
Inventors: 官振群; 王金祥
Original assignee: Guan Zhenqun; Kaishuo Bio Tech Xiamen Co ltd
Current assignee: Guan Zhenqun; Kaishuo Biotechnology Xiamen Co ltd
Priority date: 2018-06-04
Filing date: 2018-06-04
Publication date: 2021-02-26
Anticipated expiration: 2038-06-04
Also published as: CN110551712A

Abstract

When a displacement mechanism drives a piston platform with injection pistons to move to the position above a gun head platform with gun heads in the horizontal direction, and the displacement mechanism drives the piston platform to move towards the gun head platform in the vertical direction, so that each injection piston with a positioning assembly is aligned with each gun head and then combined with the gun head, and the positioning assemblies are pushed due to the pressure of the gun heads and the injection pistons which are pressed against each other, so that part of the positioning assemblies are extruded by a limiting mechanism to be exposed out of a pipe orifice of each gun head and move towards the direction of each injection piston until the positioning assemblies are contacted with a contact assembly on a circuit board, and when the positioning assemblies exposed out of the pipe orifice of each gun head are contacted with the contact assembly, the positioning of each gun head and each injection piston is completed.

Description

Nucleic acid extraction machine table with gun head positioning device

Technical Field

The invention relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction machine with a gun head positioning device.

Background

It is a common method to analyze nucleic acids for genetic, molecular biological, or animal and plant diseases. However, as the related art is rapidly developed, the current technology of nucleic acid extraction (or "nucleic acid extraction") is well developed, such as a method of capturing nucleic acid through a special functional group of a particle and then releasing the captured nucleic acid by buffer washing.

In the prior art, after an injection piston and a tip of a nucleic acid extraction system are assembled, a probe is used to confirm whether liquid (i.e., a sample solution) exists in the tip by using the tip made of graphite, i.e., to confirm that the assembly between the injection piston and the tip is completed. However, when the assembly between the tip head and the injection piston is incomplete, that is, the tip head may be assembled with the injection piston, but the tip head does not achieve complete airtightness, and although the tip head can still suck the sample solution in the test tube without complete airtightness, because the airtightness between the tip head and the injection piston is insufficient to allow the tip head to suck a sufficient volume of the sample solution, in the subsequent nucleic acid extraction step, the volume of the sample solution is insufficient to cause the detection result to cause misjudgment on heredity, molecular biology, animals, plant diseases, and the like.

In addition, when the amount of the sample solution for performing nucleic acid extraction is huge, for example, 96 groups or 144 groups, if it is found from the final detection result that there is a problem in the data presented by several sample samples after the nucleic acid extraction step is completed, it may be determined that the sample itself is abnormal, but if there is an error in the detection result, for example, because the airtightness between the injection piston and the tip is not enough for the tip to suck enough sample solution for performing nucleic acid extraction, the user can only find out the sample solution sucked by the tip from the final detection result, but cannot determine from the detection data whether there is a problem in the sample solution itself or an error in the operation of the machine, and the method of solving the problem is generally to re-sample the sample with data The nucleic acid extraction step is performed again, and whether the data of the sample solution is abnormal or not is judged through the obtained data, but the above method cannot find out the defect caused by the insufficient airtightness between the injection piston and the gun head, and the user cannot find out the problem before the nucleic acid extraction step, so that the defect is eliminated.

In addition, the currently used gun heads are mainly made of graphite, and the gun heads are discarded after being used and are not reused, so that a relatively high cost is required.

Disclosure of Invention

According to the defects of the prior art, the invention provides a nucleic acid extraction machine with a gun head positioning device, which mainly aims to arrange a positioning assembly on the inner side of a pipe orifice of one end of a gun head facing an injection piston, when the gun head is assembled with the injection piston, the positioning assembly is used for confirming that the gun head is assembled with the injection piston, and the condition that the gun head is in airtight combination with the injection piston can be further confirmed, so that the injection piston can extract enough volume of sample solution from a test tube through the gun head and enter the gun head so as to detect the sample solution with enough volume, and the defect that in the prior art, the gun head cannot absorb enough volume of sample solution from the test tube for detection due to incomplete airtightness between the gun head and the injection piston, and the detection result can possibly generate misjudgment is solved.

Another objective of the present invention is to provide a detection system for positioning a tip of a nucleic acid extraction device, wherein after an injection piston is assembled with the tip, and after the positioning assembly on the injection piston is in contact with a contact assembly of a circuit board, a signal sent by the contact assembly is used to know whether all the injection pistons are assembled with the tip and the injection pistons and the tip are in an airtight state in real time.

Another object of the present invention is to provide a method for extracting a specimen solution in a test tube, which includes the steps of positioning an injection piston having a positioning assembly and a tip head in advance before extracting the specimen solution in the test tube, and ensuring an assembling adhesion between the injection piston and the tip head, so that when the tip head extracts the specimen solution in the test tube, it is not necessary to detect the tip head made of graphite to determine whether the specimen solution exists or not, as in the prior art, to further determine whether the tip head is positioned.

A further object of the present invention is to determine the positioning between the injection piston and the tip by using the injection piston with a positioning assembly and the contact assembly disposed on the piston platform, so that the tip made of a common plastic material can be used to replace the conventional graphite tip to suck the sample solution, and the sensing assembly is not required to sense whether the tip sucks the sample solution to determine the positioning between the tip and the injection piston, so that the tip can be discarded after the detection is completed, thereby reducing the cost of the user.

According to the above object, the present invention discloses a nucleic acid extraction machine with a gun head positioning device, comprising a base, a displacement mechanism arranged on the base for moving the displacement mechanism on the base; the piston platform is arranged on the base and is pivoted with the displacement mechanism, a circuit board is arranged on one surface of the piston platform facing the base, and a plurality of contact assemblies are arranged on the circuit board; each injection piston is arranged on the piston platform and penetrates through the circuit board, so that a contact assembly is arranged around each injection piston, a limiting mechanism with a positioning assembly is arranged on the side surface of one end, facing the gun head, of each injection piston assembly, and the limiting mechanism is used for limiting the top surface of the gun head or the inner side surface of a point of the gun head, so that the positioning assembly can move and change on the limiting mechanism according to a specified path; the gun head platform is arranged on the base and is provided with a plurality of gun head placing holes; each gun head is arranged in each gun head placing hole of the gun head platform; therefore, when the displacement mechanism drives the piston platform with the injection pistons to move to the position above the gun head platform with the gun heads in the horizontal direction, and the displacement mechanism drives the piston platform to move towards the gun head platform in the vertical direction, so that each injection piston is aligned with each gun head and then combined with the gun head, and upward pushing pressure is generated, so that part of the positioning assemblies are extruded out of the pipe orifice of each gun head by the limiting mechanism due to the pushing pressure between each gun head and each injection piston and are exposed out of the pipe orifice of each gun head, and move towards the direction of each injection piston until the positioning assemblies are contacted with the contact assembly on the circuit board, and when the positioning assemblies exposed out of the pipe orifice of each gun head are contacted with the contact assembly, the positioning of each gun head and each injection piston is completed.

Drawings

FIG. 1 is a block diagram of a monitoring unit for monitoring a nucleic acid extractor having a tip positioning device according to the present disclosure.

Fig. 2 is a side view of an injection piston and tip assembled on a piston platform according to the teachings of the present invention.

Fig. 3 is a schematic diagram illustrating a tip positioning device on an injection piston in accordance with the teachings of the present invention.

Fig. 4 is a side view of the injection piston with a lance tip positioning device in accordance with the teachings of the present invention.

Fig. 5 is a schematic diagram illustrating the assembly and disassembly of an injection piston and lance tip within a piston platform in accordance with the teachings of the present invention.

FIG. 6 is a schematic diagram of a nucleic acid extraction apparatus with a tip positioning device according to the teachings of the present disclosure.

Detailed Description

So that the manner in which the above recited features and advantages of the present invention can be understood and attained by a person skilled in the art, a more particular description of the invention, briefly summarized above, may be had by reference to the appended drawings, in which like reference numerals refer to like elements, features, and embodiments. The drawings referred to below are schematic representations relating to the features of the invention and are not necessarily drawn to scale. The description of the embodiments related to the present invention will not be repeated, except for those skilled in the art.

Please refer to fig. 1 first. FIG. 1 is a block diagram of a nucleic acid extractor having a positioning device for a tip of a probe head monitored by a monitoring unit. In fig. 1, a nucleic acid extraction machine 1 is electrically connected to a monitoring unit 100, wherein the nucleic acid extraction machine 1 includes an injection piston 210 disposed in a piston platform 20, a position-limiting mechanism 40 disposed on the injection piston 210 and having a positioning component 410 on a side surface facing one end of a gun head 30, the gun head 30, a circuit board 50 disposed on a surface facing a base (not shown in the figure) below the piston platform 20, and a contact component 510 disposed on the circuit board 50, wherein the contact component 510 is electrically connected to a circuit (not shown in the figure) and an electronic component (not shown in the figure) in the circuit board 30.

The monitoring unit 100 is configured to determine whether the injection piston 210 and the gun head 310 are assembled and positioned completely by receiving a signal transmitted from the positioning element 410 and the contact element 510 after contacting each other, in this embodiment, the monitoring unit 100 further includes a determining unit 1002, an alarming unit 1004, and a display unit 1006, the determining unit 1002 is configured to receive the signal transmitted from the positioning element 410 and the contact element 510 after contacting each other, and when the positioning element 410 and the contact element 510 are contacted and transmitted, the signal transmitted confirms that the injection piston 210 and the gun head 310 are assembled and positioned completely; when the nucleic acid extracting apparatus 1 starts to operate, but the monitoring unit 100 does not receive the signal transmitted from the contact element 510, the monitoring unit 100 can determine that the assembly between the injection plunger 210 and the nozzle head 310 is not completed. In addition, the warning unit 1004 sends an abnormal signal, such as a light flashing or a sound through a buzzer, to remind the user that the current nucleic acid extractor 1 has an abnormal state, which needs to be eliminated in real time, according to the signal transmitted by the determining unit 1002 through the contact element 510, if the signal indicates that the injection piston 210 and the gun head 310 are not assembled and are not positioned. The display unit 1006 is used for displaying the abnormal signal and the current operation status of the nucleic acid extracting machine 1, and it should be noted that, after the assembly between the injection piston 210 and the gun head 310 is completed and the positioning is confirmed, the assembly status between each injection piston 210 and the gun head 310 can be monitored and checked through the display unit 1006.

Please refer to fig. 2, fig. 3 and fig. 4 simultaneously. Fig. 2 is a side view schematically showing an assembly of an injection piston and a lance tip on a piston platform, fig. 3 is a side view schematically showing a lance tip positioning device provided on the injection piston, and fig. 4 is a side view schematically showing a lance tip positioning device provided on the injection piston. In the embodiment of the present invention, the circuit board 50 is disposed on the side of the plunger platform 20 facing the base 120. The plunger platform 20 has a plurality of channels 202 therein, the circuit board 50 is disposed on a surface of the plunger platform 20 facing the base 120, and the circuit board 50 has a plurality of through holes 501, such that each through hole 501 of the circuit board 50 is aligned with and corresponds to each channel 202 of the plunger platform 20, the injection plunger 210 can simultaneously pass through the channels 202 of the plunger platform 20 and the through holes 501 of the circuit board 50, and the circuit board 50 has a plurality of contact elements 510 disposed on a surface thereof facing the base 120, such that when the injection plunger 210 passes through the plunger platform 20 and the circuit board 50, the contact elements 510 are disposed adjacent to the periphery of each injection plunger 210.

In addition, in this embodiment, a limiting mechanism 40 is disposed on an outer side of a nozzle where the injection piston 210 is assembled with the gun head 310, the limiting mechanism 40 is used to limit a top surface of the gun head 310 or a point inner side surface of the gun head 310, a positioning assembly 410 is disposed in the limiting mechanism 40, the positioning assembly 410 can move and change in the limiting mechanism 40 along a specified path, when the gun head 310 is assembled with the injection piston 210, the injection piston 210 faces the gun head 310 and is disposed on a side surface of one end of the injection piston 210, the limiting mechanism 40 having the positioning assembly 410 generates an upward pushing pressure due to a pressing pressure of the assembly between the gun head 310 and the injection piston 210, so that the positioning assembly 410 in the limiting mechanism 40 moves from the nozzle of the gun head 310 toward the injection piston 210 (i.e., the Y direction) until the positioning assembly 410 contacts with the contact assembly 510 on the circuit board 50, when the positioning element 410 contacts the contact element 510 on the circuit board 50, which means that the assembly between the injection piston 210 and the tip 310 is completed, not only the assembly is completed, but also the positioning between the injection piston 210 and the tip 310 and the tight assembly between the injection piston 210 and the tip 310 can be confirmed by the contact between the positioning element 410 and the contact element 510.

Therefore, when the injection plunger 210 is assembled with the lance tip 310, if the positioning assembly 410 disposed on the side of the injection plunger 210 facing the end of the lance tip 310 does not contact the contact assembly 510 of the circuit board 50 above, it indicates that the assembly between the injection plunger 210 and the gun head 310 is incomplete, and at this time, the monitoring unit 100 connected to the circuit board 50 cannot receive the signal transmitted after the positioning assembly 410 is in contact with and conducted by the contact assembly 510 of the circuit board 50, the judging unit 1002 of the monitoring unit 100 judges whether the state between the injection plunger 210 and the gun head 310 is abnormal, and if so, the determination unit 1002 will send an abnormal signal to the alarm unit 1004, the alarm unit 1004 will issue an alarm message, such as beeping and/or synchronized abnormal signals, are displayed on the display unit 1006 to inform the user of the current operation status in real time, so as to eliminate the abnormal signals in real time.

In another embodiment of the present invention, the piston platform may be directly formed from the circuit board assembly, as shown in FIG. 3. In fig. 3, the circuit board assembly as the piston platform of the present invention is composed of a circuit board upper cover 50a, a circuit board lower cover 50c and a circuit board 50b, wherein the circuit board 50b is disposed between the circuit board upper cover 50a and the circuit board lower cover 50c, the circuit board upper cover 50a, the circuit board lower cover 50c and the circuit board 50b respectively have a plurality of through

holes

502a,502b,502c corresponding to each other, and the inner diameter of each of the

through holes

502a,502b of the circuit board upper cover 50a and the circuit board 50b is slightly larger than the outer diameter of the injection piston 210, so that the injection piston 210 can pass through the circuit board upper cover 50a and the circuit board 50b, and one end of the injection piston 210 facing the gun head 310 can be exposed outside the circuit board 50 b. In addition, the inner diameter of each through hole 501c of the circuit board lower cover 50c is slightly larger than the outer diameter of the gun head 310 (not shown in the figures), so that the nozzle of the gun head 310 at the end facing the injection piston 210 can pass through each through hole 502c of the circuit board lower cover 50c to be assembled with the end of the injection piston 210 exposing the circuit board 50 b. In addition, a plurality of contact elements 510 are disposed around each through hole 502b of the circuit board 50b on the surface of the circuit board 50b facing the circuit board lower cover 50c, so that after the injection plunger 210 can pass through the circuit board upper cover 50a and the circuit board 50b, the contact elements 510 are disposed around each injection plunger 210. In an embodiment of the present invention, the contact members 510 may be gold fingers, metal wedges, or metal sheets, which are provided in a number corresponding to the number of the positioning members 410, and if the positioning members 410 provided on the injection piston 210 are two, the contact members 510 are two; if there are three positioning elements 410, there are three contact elements 510. It should be noted that when the positioning assembly 410 disposed in the limiting mechanism 40 is not used, i.e., the injection piston 210 is not assembled with the lance tip 310, the positioning assembly 410 in the limiting mechanism 40 is in a downward loose state according to the original gravity of the positioning assembly 410.

Therefore, when the injection piston 210 is assembled with the gun head 310, due to the tight fit between the gun head 310 and the injection piston 210, the positioning assembly 410 on the side surface facing the end of the assembly of the injection piston 210 and the gun head 310 is pressed in the limiting mechanism 40 and moves towards the

circuit boards

50 and 50b, if the positioning assembly 410 is not in contact with or has poor contact with the contact assemblies 510, for example, when the number of the positioning assemblies is three, when the injection piston 210 is assembled with the gun head 310, the normal state is that the three positioning assemblies 410 are respectively in contact with the three contact assemblies 510 on the

circuit boards

50 and 50b, which indicates that the assembly between the injection piston 210 and the gun head 310 is completed, but if only one or two positioning assemblies 410 are in contact with the contact assemblies 510, the assembly between the injection piston 210 and the gun head 310 is incomplete, which may have a gap between the assembly of the injection piston and the gun head, or the position deviation, which will cause that the reagent in the reagent tank 70 (as shown in fig. 6) cannot be sucked by a sufficient amount for the subsequent nucleic acid extraction process when the reagent is sucked by the gun head 310, at this time, the monitoring unit 100 will send an abnormal signal to notify the user of the abnormal operation of the current nucleic acid extractor 1 in real time, so that the user can eliminate the problem in real time, and avoid the problem that the data is found after the whole set of sample solutions have been subjected to nucleic acid extraction and the concentration of the sample solutions is measured, thereby further avoiding that the sample solution itself cannot be judged to be an abnormal sample solution or the sample solution is carelessly lost in manual operation after the fact, and the sample solution for performing nucleic acid extraction in the batch needs to perform the nucleic acid extraction and purification reaction again.

In an embodiment of the present invention, the channels 202 on the plunger platform 210 may be 96 channels, 144 channels or even more channels to place the injection plunger 210, as shown in fig. 4, and perform the subsequent steps of nucleic acid extraction or purification, thereby obtaining the detection data of all samples to be tested quickly and with minimal environmental impact.

Please refer to fig. 5. Fig. 5 is a schematic diagram further illustrating the assembly and disassembly of the injection piston and lance tip within the piston platform. Looking from the right to the left in the drawing of fig. 5, the rightmost drawing shows the state in which the injection piston 210 is not yet assembled with the lance tip 310; in fig. 5, the second drawing on the right shows the direction (Y-axis direction) in which the push rod 2102 of the injection piston 210 faces the gun head 310, so that the injection piston 210 is assembled with the gun head 310; the second drawing on the left in fig. 5 shows that the assembly of the injection plunger 210 with the tip end 310 is completed, that is, the tip end 310 is assembled with the injection plunger 210 upward in the Y direction (arrow direction); in fig. 5, the leftmost drawing is a schematic view showing the injection plunger 210 withdrawing the lance tip 310 apart from the injection plunger 210.

Therefore, according to the above description, when the injection plunger 210 is assembled with the tip 310, whether the assembly is completed or the assembly is correct can be determined through the contact and conduction signals between the positioning element 410 on the side surface of the injection plunger 210 facing the tip 310 and the contact element 510 of the circuit board 50. If the monitoring unit 100 receives the conducting signal between the positioning component 410 and the contact component 510, it can be confirmed that the injection piston 210 and the gun head 310 are positioned and completely assembled; on the contrary, if the monitoring unit 100 does not receive the conduction signal between the positioning component 410 and the contact component 510, it indicates that the assembly between the injection piston 210 and the gun head 310 is incomplete, at this time, the determining unit 1002 will transmit an abnormal signal to the warning unit 1004, and the warning unit 1004 will issue a warning message, such as a buzzer sound and/or display the abnormal signal in the display unit 1006 synchronously, so as to notify the user of the current operation condition in real time and eliminate the abnormal signal in real time.

Therefore, according to the above, the nucleic acid extracting machine 1 having the lance tip positioning device is shown in FIG. 6. In FIG. 6, a nucleic acid extraction machine 1 having a tip positioning device includes a base 120, a displacement mechanism 110, a piston platform 20, and a circuit board 50 (shown in FIG. 3) disposed on a surface of the piston platform 20 facing the base 120, an injection piston 210 (shown in FIG. 3) disposed in the piston platform 20, a tip platform 30, and a tip 310 disposed in the tip platform 30, wherein the displacement mechanism 110 is disposed on the base 120 and pivotally connected to the base 120, and the piston platform 20 is pivotally connected to a guide rail 1102 on the displacement mechanism 110 by a slide assembly 202, so that the piston platform 20 can move forward and backward (Z-axis direction), leftward and rightward (X-axis direction), and upward and downward (Y-axis direction) on the guide rail 1102 of the displacement mechanism 110 by the slide assembly 202 in a space spaced apart from above the base 120. In the embodiment of the present invention, the displacement mechanism 110 provided on the base 120 is L-shaped, so that the piston platform 20 can be moved in the space above the base 120 by the L-shaped displacement mechanism 110.

In the embodiment of the present invention, the nucleic acid extraction machine 1 with the gun head positioning device further includes a hole breaking needle groove 60 with a hole breaking needle and a reagent groove 70 for placing a reagent strip. Therefore, when the nucleic acid extracting apparatus 1 starts to operate, the injection plunger 210 located in the plunger platform 20 moves to the upper side of the piercing needle groove 60 having the piercing needle and is assembled with the piercing needle. Then, the piston platform 20 moves to the Z direction above the reagent tank 70 for placing the reagent strip, and then moves downward to the Y direction, the reagent strip (not shown) in the reagent tank 70 is punched by the punching needle (not shown), and the piston platform 20 moves to the Z direction according to the previous path, so that the piston platform 20 returns to the upper side of the punching needle tank 60, and the punching needle is discharged and placed in the punching needle tank 60 by the air pressure of the piston platform 20 pushing outward. Then, the piston platform 20 moves toward the right side of the drawing of fig. 6 in the X direction, and moves to the position above the lance tip platform 30 in the drawing in the Z direction, and the piston platform 20 moves downward toward the lance tip platform 30 in the Y direction, so that the injection piston 210 in the piston platform 20 is assembled with the lance tip 310 placed on the lance tip platform 30. At this time, the positioning and assembling between the injection plunger 210 and the gun head 310 can be determined according to whether the positioning assembly 410 disposed on the side surface of the end of the injection plunger 210 assembled with the gun head 310 is in contact with the circuit board 50 (as shown in fig. 2) below the plunger platform 210 or the contact assembly 510 of the circuit board 50b (as shown in fig. 3) in the circuit board assembly, and the detailed steps are described above, which will not be described herein.

After the gun head 310 is assembled with the injection piston 210 and positioning is determined to be completed, the piston platform 20 moves the injection piston 210 assembled with the gun head 310 to the left of the drawing of fig. 6 along the X direction to the left of the drawing of fig. 6, and inserts each gun head 310 downwards along the Y direction into the reagent strip with the broken hole to absorb the reagent, and then moves the piston platform 20 to the upper part of the reaction tank 80, inserts the gun head 310 with the absorbed reagent into the reaction tank 80 to perform purification reaction with the sample solution in the reaction tank 80, and the purification reaction is the same as the existing nucleic acid extraction and purification reaction, which is not further described.

It should be noted that, after the nucleic acid extraction and purification reaction is completed, since in the present invention, the positioning between the injection piston 210 and the tip 310 is determined according to the circuit conducting signal after the positioning component 410 contacts with the contact component 510 disposed on the circuit board 50, and is not determined by the current use of a detector to sense the reagent amount in the reagent strip sucked by the tip 310 made of graphite, the problem that in the prior art, when the tip 310 and the injection tip 210 are not completely assembled, the tip 310 can still suck the reagent in the reagent strip, the detector will not send an abnormal signal as long as it senses the existence of liquid, however, because the assembly between the tip 310 and the injection tip 210 is incomplete, the sucked reagent amount or the amount of buffer solution and the amount of solution in the nucleic acid separation are not enough to perform the purification reaction, or the amount after purification is very small, the problem of erroneous judgment easily caused by incomplete data is greatly reduced, the problem of the operation of the nucleic acid extraction machine 1 can be eliminated in real time, the problem of data of the sample solution can be only processed when the data of the sample solution is found to be defective after the purification reaction and the concentration detection of the whole group of sample solutions such as 96 groups of sample solutions are finished, and the waste of materials and manpower can be avoided as well as saved time.

In addition, since the present invention does not use a detector to detect whether the liquid is sucked in the lance tip 310, the material of the lance tip 310 can be changed from expensive graphite to plastic, which can greatly save the use cost of the user, and therefore, after the lance tip 310 is used, the injection piston 210 can withdraw the lance tip 310 by using the lance withdrawing plate 24 (as shown in fig. 3), and separate from the injection piston 210 and discard the lance tip 310 into the waste tank 90.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention; while the foregoing is directed to embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.

Claims

1. A nucleic acid extraction machine with a gun head positioning device comprises:

a base;

a displacement mechanism provided on the base such that the displacement mechanism moves on the base;

the gun head platform is arranged on the base and is provided with a plurality of gun head placing holes;

the plurality of gun heads are arranged in the gun head placing holes of the gun head platform;

the piston platform is arranged on the base and is pivoted with the displacement mechanism, the piston platform is provided with a plurality of channels penetrating through the piston platform, a circuit board is arranged on one surface of the piston platform, facing the base, and is provided with a plurality of through holes, each through hole corresponds to each channel of the piston platform, and a plurality of contact assemblies are arranged on one surface of the circuit board, facing the base, and the periphery of each through hole;

the injection device comprises a piston platform, a plurality of injection pistons, a circuit board and a positioning assembly, wherein the piston platform is arranged in the piston platform through each channel on the piston platform, the circuit board is arranged in a penetrating manner, the contact assembly is arranged around each injection piston, a limiting mechanism is arranged on the outer side of a pipe orifice of each injection piston and each gun head assembly, the limiting mechanism is used for limiting the top surface of the gun head or the inner side surface of the point of the gun head, the positioning assembly is arranged in the limiting mechanism, and the positioning assembly is arranged on the limiting mechanismInner partCan move and change according to the appointed path;

therefore, when the displacement mechanism drives the piston platform with the injection pistons to move to the position above the gun head platform with the gun heads in the horizontal direction, and the displacement mechanism drives the piston platform to move towards the gun head platform in the vertical direction, so that each injection piston is aligned with each gun head and assembled, each gun head is in tight contact with the limiting mechanism with the positioning assembly arranged on the side surface of one end, facing towards each gun head, of each injection piston, and upward pushing pressure is generated, so that part of the positioning assemblies in the limiting mechanism are extruded by the limiting mechanism due to the pushing pressure between each gun head and each injection piston to be exposed out of the pipe orifice of each gun head and move towards the direction of each injection piston until the positioning assemblies are in contact with the contact assemblies on the circuit board, when the positioning assembly exposed out of the nozzle of each gun head is contacted with the contact assembly, the positioning of each gun head and each injection piston is completed.

2. The nucleic acid extraction machine with the lance tip positioning device of claim 1, wherein the positioning assembly is a conductor.

3. The nucleic acid extraction machine with the lance tip positioning device of claim 1 or 2, wherein the positioning assembly is a ball.

4. The nucleic acid extraction machine station with a lance tip positioning device of claim 1, further comprising a sealing ring disposed on an end of each injection piston facing the nozzle of each lance tip.

5. The nucleic acid extraction machine station with the lance head positioning device of claim 1, wherein the limiting mechanism further comprises a positioning assembly for adjusting the return positioning when the lance head moves.

6. The nucleic acid extraction machine with the lance head positioning device of claim 1, further comprising a monitoring unit electrically connected to the circuit board for receiving a signal when the positioning assembly contacts the contact assembly.

7. The apparatus of claim 6, wherein the monitoring unit further comprises a display unit for displaying a current assembly status of each injection piston and each lance tip.

8. The nucleic acid extractor apparatus according to claim 6, wherein the monitoring unit further comprises an alarm unit, wherein the alarm unit sends an alarm signal when the positioning member exposed to the nozzle of each of the tips is not in contact with the contact member.