CN110543004A - Digital microscope objective with high magnification and large zoom ratio - Google Patents
Digital microscope objective with high magnification and large zoom ratio Download PDFInfo
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- CN110543004A CN110543004A CN201910797532.5A CN201910797532A CN110543004A CN 110543004 A CN110543004 A CN 110543004A CN 201910797532 A CN201910797532 A CN 201910797532A CN 110543004 A CN110543004 A CN 110543004A
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- 230000007547 defect Effects 0.000 abstract description 3
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- 238000001000 micrograph Methods 0.000 description 3
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B15/00—Optical objectives with means for varying the magnification
- G02B15/14—Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective
- G02B15/16—Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective with interdependent non-linearly related movements between one lens or lens group, and another lens or lens group
- G02B15/163—Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective with interdependent non-linearly related movements between one lens or lens group, and another lens or lens group having a first movable lens or lens group and a second movable lens or lens group, both in front of a fixed lens or lens group
- G02B15/167—Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective with interdependent non-linearly related movements between one lens or lens group, and another lens or lens group having a first movable lens or lens group and a second movable lens or lens group, both in front of a fixed lens or lens group having an additional fixed front lens or group of lenses
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
- G02B21/025—Objectives with variable magnification
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Abstract
The invention discloses a high-magnification large-zoom-ratio digital microscope objective lens, which comprises a first lens group (2) with positive focal power, a second lens group (3) with positive focal power, a third lens group (4) with negative focal power and a fourth lens group (5) with negative focal power, which are sequentially arranged between an object surface (1) and an image surface (6); the first lens group (2) and the fourth lens group (5) are fixed groups; the second lens group (3) is a zoom group, and different magnifications and continuous zoom of the magnifications are realized by moving along the optical axis O; the third lens group (4) is a compensation group for performing magnification compensation for magnification variation by moving along the optical axis O. The magnification of the objective lens is as low as 4 to 6 and as high as 32 to 48, and the zoom ratio is more than 8, so that the objective lens can be used for observing details of objects with different sizes, and overcomes the common defect that the selection of the magnification of the existing high-magnification microscope is limited.
Description
Technical Field
The invention relates to the technical field of microscopes, in particular to a digital microscope objective with high magnification and large zoom ratio.
Background
a microscope is an optical device that magnifies a minute object or a minute part of an object at a high magnification to facilitate observation or detection. The continuous variable magnification microscope is a microscope whose magnification can be continuously changed within a certain range, and can more conveniently observe the fine structures of objects with different sizes.
The current continuous zoom microscope is mainly a stereoscopic microscope, the zoom ratio of which can reach 10 times or more, but the magnification is mostly in the range of 0.5X to 7X, and the magnification is low.
Other types of microscopes, such as biological or metallurgical microscopes, have few continuous variable magnification objectives. In order to realize the observation requirements of different details of an object, the observation needs are often realized by installing objective lenses with different magnifications on an objective lens converter by means of the objective lens converter. But the disadvantage is that the choice of magnification is limited, and only about 6 kinds of fixed magnification are available.
The patent specification with the publication number of CN 106842531A discloses a microscope objective lens capable of continuously zooming, which comprises an objective lens seat, a zooming group and a compensation group, wherein the zooming group and the compensation group are arranged in a cavity of the objective lens seat and can move along the axial direction of the cavity; through adopting the structure that the zoom base is movably inserted into the compensation base, when the zoom group and the compensation group move in the cavity in the objective lens base, the zoom group and the compensation group can always keep good concentricity, thereby ensuring the stability of the objective lens. Although the technical scheme can realize continuous zooming, the aim is to ensure that the concentricity between the zooming group and the compensation group is high and the movement stability is high. There is no specific continuous magnification effect of the microscope objective. Moreover, the above technical solutions do not specifically describe the lens combination, structural features and the like of the variable power group and the compensation group.
The digital microscope images the object image seen by the microscope on the screen of the microscope or on a computer through an image sensor.
The main benefits of digital microscopes are: the conventional optical microscope can only be used by one person, and is difficult to share the microscope image, and special instruments are often needed for taking the image in the microscope. However, the digital microscope can display the microscope image directly to the microscope's own screen via a sensor or transmit to a computer for further analysis and processing.
therefore, it is necessary to develop a continuous zoom microscope objective lens with high magnification and large zoom ratio for a digital microscope to fill the market vacancy.
Patent specification CN 201242610Y discloses a zoom digital camera micro objective lens, which comprises thirteen lens sets arranged in sequence to form an objective lens group and a zoom lens group with a common linear distance of ∞, wherein the zoom lens group comprises a front fixed group, a zoom group, a rear fixed group and a compensation group arranged in sequence. The zoom ratio of the continuous zoom digital video-recording micro objective can reach 8 times.
Disclosure of Invention
Aiming at the defects in the field, the invention provides the digital microscope objective with high magnification and large zoom ratio, which is used for observing details of objects with different sizes and overcomes the common defect that the selection of the magnification of the existing high-magnification microscope is limited.
a digital microscope objective lens with high magnification and large zoom ratio comprises a first lens group with positive focal power, a second lens group with positive focal power, a third lens group with negative focal power and a fourth lens group with negative focal power, which are sequentially arranged between an object plane and an image plane;
The first lens group and the fourth lens group are fixed groups;
the second lens group is a zoom group, and different magnifications and continuous zoom of the magnifications are realized by moving along the optical axis O;
the third lens group is a compensation group for performing magnification compensation for magnification variation by moving along the optical axis O.
The change of the magnification of the high-magnification high-variable-magnification-ratio digital microscope objective lens is realized by the change of the intervals among the components. The objective lens realizes the zooming function through two fixed groups and two movable groups, wherein the fixed groups are a front fixed group (a first lens group) and a rear fixed group (a fourth lens group), and the movable groups are a zooming group and a compensation group. The distance between the front fixed group and the object plane is fixed and unchanged, the variable magnification group moves linearly with the change of the magnification, the moving distance and the change of the magnification are in a linear relation, the compensation group moves nonlinearly with the change of the magnification, the moving distance and the change of the magnification are in a nonlinear relation, magnification compensation is carried out on magnification change generated by the displacement of the variable magnification group, and the rear fixed group keeps the position of the image plane unchanged and corrects aberration.
The high-magnification and large-zoom-ratio digital microscope objective lens has the magnification ratio as low as 4 to 6 and as high as 32 to 48, and the zoom ratio is more than 8.
Preferably, the first lens group is a front fixed group, and the distance between the first lens group and the object plane is fixed and unchanged;
The first lens group is composed of a plano-convex lens with positive focal power, a plano-concave lens with negative focal power and a biconvex lens with positive focal power;
The plano-concave lens and the biconvex lens are gluing components.
Preferably, the second lens group is composed of a double convex lens having positive power and a meniscus lens having negative power;
The double convex lens and the meniscus lens are a gluing assembly;
the moving distance of the second lens group is 20-30 mm.
preferably, the third lens group is composed of a meniscus lens having a negative power, a meniscus lens having a negative power;
The meniscus lens and the meniscus lens are a gluing assembly;
The moving distance of the third lens group is 70-80 mm.
Preferably, the fourth lens group is a rear fixed group, and the distance between the fourth lens group and the image plane is constant;
the fourth lens group is composed of a biconcave lens with negative focal power, a meniscus lens with negative focal power and a biconvex lens with positive focal power;
the meniscus lens and the double convex lens are a gluing assembly.
Preferably, the high-magnification large-zoom-ratio digital microscope objective lens satisfies the following conditions:
0.2<|f1/f|<2.2;
0.5<|f2/f|<6.1;
0.8<|f3/f|<8.9;
2.0<|f4/f|<23.3;
Wherein f is the total focal length of the digital microscope objective lens, f1 is the focal length of the first lens group, f2 is the focal length of the second lens group, f3 is the focal length of the third lens group, and f4 is the focal length of the fourth lens group.
Preferably, the minimum spacing between the first lens group and the second lens group is 5 mm;
The minimum interval between the second lens group and the third lens group is 4 mm.
preferably, the focal length range of the high-magnification high-zoom-ratio digital microscope objective lens is 5-61 mm, and the working distance is 5-15 mm.
preferably, the high-magnification high-zoom-ratio digital microscope objective further comprises a diaphragm arranged at the first meniscus lens of the third lens group, and the aperture of the diaphragm is kept unchanged in the zooming process.
The distance between the front fixed group and the object plane is fixed and unchanged, so that the working distance (namely the distance between the surface of the front lens of the objective lens and the object to be detected) under different magnifications is constant and reaches more than 5 mm; after the aberration is corrected by the rear fixed group, the distortion of all zoom positions is controlled within 1 percent, and the method can be used for observation and measurement.
The aperture of the diaphragm is kept unchanged in the zooming process, but the object-side numerical aperture of the whole objective lens changes along with the change of the magnification, so that the requirements of different numerical apertures of a microscope system under different magnifications are met.
compared with the prior art, the invention has the main advantages that: the design of the invention realizes the continuous zoom of the optical magnification, the magnification is as low as 4 to 6 and as high as 32 to 48, the zoom ratio can reach more than 8 times, and the digital microscope objective lens with high magnification and large zoom ratio has clear imaging, simple structure, convenient use and constant working distance. The objective lens realizes continuous zooming, simultaneously solves the inconvenience that the traditional microscope needs to switch the objective lens and the ocular lens, can be directly matched with an image sensor for further analysis and processing, and realizes the digitization of the microscope.
Drawings
FIG. 1 is a schematic diagram of a digital microscope objective with different optical magnification settings;
FIG. 2 is a minimum magnification (5 ×) Modulation Transfer Function (MTF) plot;
FIG. 3 is a graph of MTF at intermediate magnification (20 ×);
FIG. 4 is a maximum magnification (40X) MTF graph;
FIG. 5 is a plot of minimum magnification (5X);
FIG. 6 is a plot of intermediate magnification (20X);
Fig. 7 is a plot of maximum magnification (40 ×).
Detailed Description
The invention is further described with reference to the following drawings and specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The following examples are conducted under conditions not specified, usually according to conventional conditions, or according to conditions recommended by the manufacturer.
As shown in fig. 1, the objective lens of the high magnification and large zoom ratio digital microscope of the present embodiment sequentially includes, in a direction from an object plane 1 to an image plane 6: the lens comprises an object plane 1, a first lens group 2 with positive focal power, a second lens group 3 with positive focal power, a third lens group 4 with negative focal power, a fourth lens group 5 with negative focal power, protective glass 7 and an image plane 6.
the first lens group 2 and the fourth lens group 5 are fixed groups.
the second lens group 3 is a variable magnification group that realizes different magnifications and continuous magnification variation of the magnifications by moving along the optical axis O.
The third lens group 4 is a compensation group that performs magnification compensation for magnification variation by moving along the optical axis O.
the objective lens of the embodiment is suitable for the field of digital microscopes, and can realize a large zoom ratio of 8 times by continuously changing the optical magnification from 5 to 40.
the first lens group 2 is a front fixed group and is composed of a plano-convex lens 21 with positive focal power, a plano-concave lens 22 with negative focal power and a double convex lens 23 with positive focal power, wherein the plano-concave lens 22 and the double convex lens 23 are cemented components, and the distance between the lens group and the object plane 1 is fixed.
The second lens group 3 is a zoom group and comprises a double convex lens 31 with positive focal power and a meniscus lens 32 with negative focal power, wherein the double convex lens 31 and the meniscus lens 32 are gluing components, the lens group moves linearly along the optical axis O, and the moving distance of the second lens group 3 is 20-30 mm.
The third lens group 4 is a compensation group and comprises a meniscus lens 41 with negative focal power and a meniscus lens 42 with negative focal power, wherein the meniscus lens 41 and the meniscus lens 42 are gluing components, the lens group moves along the optical axis O in a curve, and the moving distance of the third lens group 4 is 70-80 mm.
The fourth lens group 5 is a rear fixed group and is composed of a double concave lens 51 with negative focal power, a meniscus lens 52 with negative focal power and a double convex lens 53 with positive focal power, wherein the meniscus lens 52 and the double convex lens 53 are cemented components, and the distance between the lens group and the image plane is fixed.
The objective lens of the embodiment satisfies the following conditions:
f1=11.2mm,
f2=31.2mm,
f3=-45.5mm,
f4=-118.8mm;
Where f1 is the focal length of the first lens assembly 2, f2 is the focal length of the second lens assembly 3, f3 is the focal length of the third lens assembly 4, and f4 is the focal length of the fourth lens assembly 5.
The aperture stop of the objective lens of the embodiment is arranged at the first meniscus lens 41 of the third lens group 4, and the aperture stop aperture remains unchanged during the zooming process.
Table 1 details the parameters corresponding to the objective lens of the example under different magnifications. The radius of curvature (R) of each surface of each lens in the objective lens of the present invention, the thickness (D) which is the distance between each surface and the previous surface, and the refractive index (n) of each element are specified. The thickness D is calculated as the distance between the intersection of each surface and the optical axis O.
TABLE 1
Serial number | radius of curvature (R/mm) | Thickness (D/mm) | refractive index (n) |
Article surface | Infinity | 5 | |
1# | Infinity | 3 | 1.8 |
2# | -9 | 6 | |
3# | Infinity | 2 | 1.9 |
4# | 14 | 4 | 1.5 |
5# | -13 | 83;56;39;27;13;5 | |
6# | 26 | 4 | 1.7 |
7# | -15 | 3 | 1.9 |
8# | -49 | 10;4;5;8;17;28 | |
9# | 19 | 3 | 1.7 |
10# | 7 | 3 | 1.9 |
11# | 9 | 22;55;70;79;84;82 | |
12# | -12 | 3 | 1.7 |
13# | 12 | 10 | |
14# | 21 | 2 | 1.8 |
15# | 9 | 3 | 1.7 |
16# | -27 | 28 | |
17# | Infinity | 1 | 1.5 |
18# | Infinity | 2 |
In table 1, the radius R is a positive number and indicates the concave image surface 6 side of the surface; the radius R is a negative number and indicates that the surface is concave to the object surface 1 side; a radius R of Infinity (Infinity) indicates that the face is a plane.
The numbers in table 1 are arranged in order of the direction from object plane 1 to image plane 6, for example: 1# and 2# are two surfaces of the plano-convex lens 21, wherein 1# is a surface close to one side of the object surface 1 and is a plane, and 2# is a surface close to one side of the image surface 6 and is concave to the object surface 1; no. 4 is a bonding surface of the plano-concave lens 22 and the biconvex lens 23, and the concave surface is towards the image surface 6; 9# is a surface of the meniscus lens 41 on the side close to the object plane 1, and is also a diaphragm position; the 18# plane is a plane on the side of the cover glass 7 closer to the image plane 6.
In table 1, the corresponding thickness D of the object plane 1 is 5mm, which means that the distance between the object plane 1 and the 1# plane of the plano-convex lens 21 is 5mm, and the distance is the working distance of the objective lens in this embodiment; the corresponding thickness D of the No. 1 surface is 3mm, which means that the distance between the No. 1 surface and the No. 2 surface is 3mm, namely the thickness of the plano-convex lens 21 is 3 mm; and so on; the corresponding thickness D of the 18# surface is 2mm, which means that the distance between the 18# surface and the image surface 6 is 2 mm.
In this embodiment, the correspondence between the object-side numerical aperture and the different magnifications is set as shown in table 2.
TABLE 2
Multiplying power | Object space numerical aperture |
5× | 0.12 |
10× | 0.14 |
15× | 0.16 |
20× | 0.20 |
30× | 0.30 |
40× | 0.40 |
In this embodiment, the MTF curves of the objective lens are shown in FIGS. 2-4. Under different magnifications, the MTF curves of the respective fields are concentrated and close to the diffraction limit. Table 3 shows the number of line pairs that can be resolved by the objective lens of the digital microscope and the objective lens of the ordinary optical microscope, and it can be seen that the number of line pairs that can be resolved by the objective lens of the present embodiment is higher, and the contrast of the imaging is better.
TABLE 3
magnification factor | Common optical microscope (lp/mm) | objective lens of the embodiment (lp/mm) |
5× | 250 | 320 |
20× | 800 | 840 |
40× | 1160 | 1400 |
in the present embodiment, the dot patterns of the objective lens are shown in fig. 5 to 7. The RMS sizes of the dot charts corresponding to different fields of view with different magnifications are all in a pixel size range, and the resolution meets the requirements of the image sensor.
in this embodiment, the objective lens achieves the following optical criteria:
Focal length: f' is 5.1-60.9 mm;
Working distance: 5.2 mm;
Distortion: < 0.55% (all zoom positions);
Resolution ratio: can match 350-500 ten thousand pixel image sensors with the size of 1/3 inches and the size of 1/3 inches.
Furthermore, it should be understood that various changes and modifications can be made by one skilled in the art after reading the above description of the present invention, and equivalents also fall within the scope of the invention as defined by the appended claims.
Claims (10)
1. A digital microscope objective lens with high magnification and large zoom ratio is characterized by comprising a first lens group (2) with positive focal power, a second lens group (3) with positive focal power, a third lens group (4) with negative focal power and a fourth lens group (5) with negative focal power, which are sequentially arranged between an object plane (1) and an image plane (6);
the first lens group (2) and the fourth lens group (5) are fixed groups;
the second lens group (3) is a zoom group, and different magnifications and continuous zoom of the magnifications are realized by moving along the optical axis O;
The third lens group (4) is a compensation group for performing magnification compensation for magnification variation by moving along the optical axis O.
2. the high magnification ratio digital microscope objective lens of claim 1, wherein the magnification is as low as 4 x to 6 x and as high as 32 x to 48 x, and the magnification ratio is 8 times or more.
3. the objective lens of a high magnification large variable magnification digital microscope according to claim 1, wherein the first lens group (2) is a front fixed group, and the distance from the object plane (1) is fixed;
the first lens group (2) is composed of a plano-convex lens (21) having positive focal power, a plano-concave lens (22) having negative focal power, and a double convex lens (23) having positive focal power;
the plano-concave lens (22) and the biconvex lens (23) are glued components.
4. A high magnification large variable power ratio digital microscope objective lens according to claim 1, characterized in that the second lens group (3) consists of a double convex lens (31) with positive power and a meniscus lens (32) with negative power;
the double convex lens (31) and the meniscus lens (32) are gluing components;
the moving distance of the second lens group (3) is 20-30 mm.
5. High magnification large variable power ratio digital microscope objective according to claim 1, characterized in that the third lens group (4) consists of a meniscus lens (41) with negative power, a meniscus lens (42) with negative power;
The meniscus lens (41) and the meniscus lens (42) are a gluing assembly;
The moving distance of the third lens group (4) is 70-80 mm.
6. The objective lens of a high magnification large variable magnification digital microscope according to claim 1, wherein the fourth lens group (5) is a rear fixed group, and the distance from the image plane (6) is fixed;
The fourth lens group (5) is composed of a biconcave lens (51) having negative power, a meniscus lens (52) having negative power, and a biconvex lens (53) having positive power;
The meniscus lens (52) and the double convex lens (53) are glued components.
7. The high magnification large zoom ratio digital microscope objective of claim 1, wherein the digital microscope objective satisfies the following conditions:
0.2<|f1/f|<2.2;
0.5<|f2/f|<6.1;
0.8<|f3/f|<8.9;
2.0<|f4/f|<23.3;
Wherein f is the total focal length of the digital microscope objective lens, f1 is the focal length of the first lens group (2), f2 is the focal length of the second lens group (3), f3 is the focal length of the third lens group (4), and f4 is the focal length of the fourth lens group (5).
8. A high magnification large variable power ratio digital microscope objective lens according to claim 1, characterized in that the minimum separation of the first lens group (2) and the second lens group (3) is 5 mm;
The minimum interval between the second lens group (3) and the third lens group (4) is 4 mm.
9. the objective lens of claim 1, wherein the digital microscope has a focal length in the range of 5-61 mm and a working distance in the range of 5-15 mm.
10. a high magnification ratio digital microscope objective lens according to claim 5, characterized by further comprising an aperture stop provided at the meniscus lens (41) of the third lens group (4), the aperture stop being of a constant aperture stop aperture during magnification.
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