CN110543004B - High-magnification large-zoom-ratio digital microscope objective lens - Google Patents

High-magnification large-zoom-ratio digital microscope objective lens Download PDF

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Publication number
CN110543004B
CN110543004B CN201910797532.5A CN201910797532A CN110543004B CN 110543004 B CN110543004 B CN 110543004B CN 201910797532 A CN201910797532 A CN 201910797532A CN 110543004 B CN110543004 B CN 110543004B
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lens
lens group
magnification
group
microscope objective
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CN110543004A (en
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余飞鸿
刘锦琳
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Hangzhou Touptek Photoelectric Technology Co ltd
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Hangzhou Touptek Photoelectric Technology Co ltd
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    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B15/00Optical objectives with means for varying the magnification
    • G02B15/14Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective
    • G02B15/16Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective with interdependent non-linearly related movements between one lens or lens group, and another lens or lens group
    • G02B15/163Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective with interdependent non-linearly related movements between one lens or lens group, and another lens or lens group having a first movable lens or lens group and a second movable lens or lens group, both in front of a fixed lens or lens group
    • G02B15/167Optical objectives with means for varying the magnification by axial movement of one or more lenses or groups of lenses relative to the image plane for continuously varying the equivalent focal length of the objective with interdependent non-linearly related movements between one lens or lens group, and another lens or lens group having a first movable lens or lens group and a second movable lens or lens group, both in front of a fixed lens or lens group having an additional fixed front lens or group of lenses
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/02Objectives
    • G02B21/025Objectives with variable magnification

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Nonlinear Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Lenses (AREA)

Abstract

The invention discloses a high-magnification large-zoom-ratio digital microscope objective lens, which comprises a first lens group (2) with positive focal power, a second lens group (3) with positive focal power, a third lens group (4) with negative focal power and a fourth lens group (5) with negative focal power, wherein the first lens group (2) with positive focal power, the second lens group (3) with positive focal power, the third lens group (4) with negative focal power and the fourth lens group (5) with negative focal power are sequentially arranged between an object plane (1) and an image plane (6); the first lens group (2) and the fourth lens group (5) are fixed groups; the second lens group (3) is a magnification-varying group, and different magnifications and continuous magnification variation of the magnifications are realized by moving along the optical axis O; the third lens group (4) is a compensation group that performs magnification compensation for magnification variation by moving along the optical axis O. The magnification of the objective lens is as low as 4-6, as high as 32-48, and the zoom ratio is more than 8, so that the objective lens can be used for observing object details with different sizes, and overcomes the common problem that the current high-magnification microscope has limited magnification selection.

Description

High-magnification large-zoom-ratio digital microscope objective lens
Technical Field
The invention relates to the technical field of microscopes, in particular to a high-magnification and high-magnification-ratio digital microscope objective lens.
Background
A microscope is an optical device that magnifies a minute object or a minute portion of an object at a high magnification to facilitate observation or detection. The microscope with continuously variable magnification is a microscope with magnification which can be continuously changed in a certain range, and can observe the microstructure of objects with different sizes more conveniently.
The current continuous variable magnification microscope is mainly a stereoscopic microscope, the variable magnification ratio of which can reach 10 times or more, but the magnification is mostly in the range of 0.5-7 x, and the magnification is lower.
Other types of microscopes, such as biological or metallographic microscopes, have few continuous magnification objectives. In order to realize the observation requirements of different details of an object, the objective lens converter is often used for realizing by installing objective lenses with different multiplying powers on the objective lens converter. However, the method has the disadvantage that the choice of magnification is limited, and only about 6 kinds of fixed magnification are adopted.
The patent specification with the publication number of CN 106842531A discloses a microscope objective lens capable of continuously changing magnification, which comprises an objective lens seat, a magnification changing group and a compensation group, wherein the magnification changing group and the compensation group are arranged in a cavity of the objective lens seat and can move along the axial direction of the cavity; through adopting the structure of variable magnification seat and compensation seat activity grafting, when variable magnification group and compensation group remove in the cavity in the objective seat, variable magnification group and compensation group can remain fine concentricity all the time to can guarantee the stability of objective. The technical scheme can realize continuous zooming, but mainly aims to ensure high concentricity and high movement stability between the zooming group and the compensation group. The specific continuous magnification-varying effect of the microscope objective is not of any concern. Moreover, the above technical solutions do not specifically describe the lens combinations and structural features of the variable power group and the compensation group, etc.
The digital microscope is to image the image of the object seen by the microscope on the screen or computer of the microscope through the image sensor.
The digital microscope has the main advantages that: conventional optical microscopes are only available for one person, and it is difficult to share the images of the microscope, and special equipment is often required to capture the images in the microscope. However, digital microscopes can display microscopic images directly to the microscope's own screen via sensors or to a computer for further analysis and processing.
Therefore, it is needed to develop a high-magnification and high-magnification-ratio continuous magnification microscope objective lens for a digital microscope to fill the market gap.
The patent specification with the publication number of CN 201242610Y discloses a continuous variable magnification digital photographing microscope objective lens, which comprises thirteen lenses which are sequentially arranged to form an object receiving lens group and a zoom lens group with the common track distance of +.. The zoom ratio of the continuous zoom digital photographing microscope objective can reach 8 times.
Disclosure of Invention
Aiming at the defects in the field, the invention provides a high-magnification large-magnification-ratio digital microscope objective lens which is used for observing object details with different sizes and overcomes the common problem that the magnification selection of the existing high-magnification microscope is limited.
A high magnification large zoom ratio digital microscope objective lens comprises a first lens group with positive focal power, a second lens group with positive focal power, a third lens group with negative focal power and a fourth lens group with negative focal power which are sequentially arranged between an object plane and an image plane;
the first lens group and the fourth lens group are fixed groups;
The second lens group is a magnification-varying group, and different magnifications and continuous magnification variation of the magnifications are realized by moving along the optical axis O;
The third lens group is a compensation group that performs magnification compensation for magnification variation by moving along the optical axis O.
The magnification of the high magnification large variable magnification digital microscope objective lens is changed by means of the change of the interval between the components. The objective lens realizes the magnification changing function through two fixed groups and two movable groups, wherein the fixed groups comprise a front fixed group (a first lens group) and a rear fixed group (a fourth lens group), and the movable groups comprise a magnification changing group and a compensating group. The distance between the front fixed group and the object plane is fixed, the zoom group moves linearly along with the change of the magnification, the moving distance and the change of the magnification are in linear relation, the compensation group moves non-linearly along with the change of the magnification, the moving distance and the change of the magnification are in non-linear relation, the magnification compensation is carried out on the change of the magnification generated by the displacement of the zoom group, and the rear fixed group keeps the position of the image plane unchanged and corrects the aberration.
The high-magnification large-zoom-ratio digital microscope objective lens has a magnification as low as 4 to 6, and a magnification as high as 32 to 48, and the zoom ratio is more than 8.
Preferably, the first lens group is a front fixed group, and the distance between the first lens group and the object plane is fixed;
The first lens group consists of a plano-convex lens with positive focal power, a plano-concave lens with negative focal power and a biconvex lens with positive focal power;
the plano-concave lens and the biconvex lens are glued components.
Preferably, the second lens group is composed of a biconvex lens having positive optical power and a meniscus lens having negative optical power;
The biconvex lens and the meniscus lens are glued components;
the moving distance of the second lens group is 20-30 mm.
Preferably, the third lens group is composed of a meniscus lens having negative power, a meniscus lens having negative power;
The meniscus lens and the meniscus lens are glued components;
the moving distance of the third lens group is 70-80 mm.
Preferably, the fourth lens group is a rear fixed group, and the distance between the fourth lens group and the image plane is fixed;
the fourth lens group consists of a biconcave lens with negative focal power, a meniscus lens with negative focal power and a biconvex lens with positive focal power;
the meniscus lens and the biconvex lens are glued components.
Preferably, the high-magnification digital microscope objective lens satisfies the following conditions:
0.2<|f1/f|<2.2;
0.5<|f2/f|<6.1;
0.8<|f3/f|<8.9;
2.0<|f4/f|<23.3;
Wherein f is the total focal length of the digital microscope objective lens, f1 is the focal length of the first lens group, f2 is the focal length of the second lens group, f3 is the focal length of the third lens group, and f4 is the focal length of the fourth lens group.
Preferably, the minimum interval between the first lens group and the second lens group is 5mm;
The minimum interval between the second lens group and the third lens group is 4mm.
Preferably, the focal length range of the high-magnification variable-ratio digital microscope objective lens is 5-61 mm, and the working distance is 5-15 mm.
Preferably, the high-magnification variable-ratio digital microscope objective further comprises a diaphragm arranged at the first meniscus lens of the third lens group, and the aperture of the diaphragm is kept unchanged in the variable-magnification process.
The distance between the front fixed group and the object plane is fixed, so that the working distance (namely the distance between the surface of the front lens of the objective lens and the detected object) is constant and reaches more than 5mm under different magnification; after correcting aberration by the rear fixed group, the distortion of all zoom positions is controlled to be within 1%, and the method can be used for observation and measurement.
The aperture of the diaphragm is kept unchanged in the zooming process, but the numerical aperture of the object space of the whole objective lens is changed along with the change of magnification, so that the aperture meets the requirements of different numerical apertures of a microscope system under different magnifications.
Compared with the prior art, the invention has the main advantages that: the design of the invention realizes the continuous magnification change of the optical magnification, the magnification is as low as 4 to 6 and as high as 32 to 48, the magnification ratio can reach more than 8 times, and the high magnification and large magnification ratio digital microscope objective lens has clear imaging, simple structure, convenient use and constant working distance. The objective lens realizes continuous zoom, solves the inconvenience that the traditional microscope needs to switch the objective lens and the ocular lens, and can be directly matched with an image sensor to carry out further analysis and processing, thereby realizing the digitization of the microscope.
Drawings
FIG. 1 is a schematic diagram of a digital microscope objective lens with different optical power settings;
FIG. 2 is a graph of minimum magnification (5×) Modulation Transfer Function (MTF);
FIG. 3 is a graph of the intermediate magnification (20×) MTF;
Fig. 4 is a maximum magnification (40×) MTF plot;
FIG. 5 is a plot of minimum magnification (5X) points;
FIG. 6 is a plot of intermediate magnification (20X) points;
fig. 7 is a maximum magnification (40×) dot column diagram.
Detailed Description
The invention will be further elucidated with reference to the drawings and to specific embodiments. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. The methods of operation, under which specific conditions are not noted in the examples below, are generally in accordance with conventional conditions, or in accordance with the conditions recommended by the manufacturer.
As shown in fig. 1, the high-magnification digital microscope objective lens of the present embodiment sequentially includes, in a direction from an object plane 1 to an image plane 6: an object plane 1, a first lens group 2 having positive power, a second lens group 3 having positive power, a third lens group 4 having negative power, a fourth lens group 5 having negative power, a cover glass 7, and an image plane 6.
The first lens group 2 and the fourth lens group 5 are fixed groups.
The second lens group 3 is a magnification-varying group, and different magnifications and continuous magnification variation of the magnifications are achieved by moving along the optical axis O.
The third lens group 4 is a compensation group that performs magnification compensation for magnification variation by moving along the optical axis O.
The objective lens of the embodiment is suitable for the field of digital microscopes, can realize continuous change of 5-40 times of optical magnification, and achieves a large zoom ratio of 8 times.
The first lens group 2 is a front fixed group and consists of a plano-convex lens 21 with positive focal power, a plano-concave lens 22 with negative focal power and a biconvex lens 23 with positive focal power, wherein the plano-concave lens 22 and the biconvex lens 23 are glued components, and the distance between the lens group and the object plane 1 is fixed.
The second lens group 3 is a power-variable group composed of a biconvex lens 31 having positive power and a meniscus lens 32 having negative power, wherein the biconvex lens 31 and the meniscus lens 32 are a cemented assembly, and the lens group moves linearly along the optical axis O, and the moving distance of the second lens group 3 is 20 to 30mm.
The third lens group 4 is a compensation group composed of a meniscus lens 41 having negative power and a meniscus lens 42 having negative power, wherein the meniscus lens 41 and the meniscus lens 42 are a cemented assembly, and the lens group moves along the optical axis O in a curve, and the moving distance of the third lens group 4 is 70 to 80mm.
The fourth lens group 5 is a rear fixed group, and is composed of a biconcave lens 51 having negative optical power, a meniscus lens 52 having negative optical power, and a biconvex lens 53 having positive optical power, wherein the meniscus lens 52 and the biconvex lens 53 are cemented components, and the distance between the lens group and the image plane is fixed.
The objective lens of the present embodiment satisfies the following conditions:
f1=11.2mm,
f2=31.2mm,
f3=-45.5mm,
f4=-118.8mm;
Wherein f1 is a focal length of the first lens group 2, f2 is a focal length of the second lens group 3, f3 is a focal length of the third lens group 4, and f4 is a focal length of the fourth lens group 5.
The aperture of the example objective lens is arranged at the first meniscus lens 41 of the third lens group 4, the aperture of the aperture being kept unchanged during magnification.
Table 1 details the parameters corresponding to the different magnifications of the objective lens of the example. In the objective lens of the present invention, the radius of curvature (R) of each surface of each lens, the distance between each surface and the front surface, that is, the thickness (D), and the refractive index (n) of each element are defined. The thickness D is calculated as the distance between the intersection of the faces and the optical axis O.
TABLE 1
Sequence number Radius of curvature (R/mm) Thickness (D/mm) Refractive index (n)
Object plane Infinity 5
1# Infinity 3 1.8
2# -9 6
3# Infinity 2 1.9
4# 14 4 1.5
5# -13 83;56;39;27;13;5
6# 26 4 1.7
7# -15 3 1.9
8# -49 10;4;5;8;17;28
9# 19 3 1.7
10# 7 3 1.9
11# 9 22;55;70;79;84;82
12# -12 3 1.7
13# 12 10
14# 21 2 1.8
15# 9 3 1.7
16# -27 28
17# Infinity 1 1.5
18# Infinity 2
In table 1, the radius R is a positive number, and represents the concave direction of the surface toward the image surface 6 side; radius R is a negative number, indicating that the face is concave to the object plane 1 side; radius R is Infinity (Infinity) indicating that the face is planar.
The numbers in table 1 are arranged in the order of the direction from object plane 1 to image plane 6, for example: 1# and 2# are two surfaces of the plano-convex lens 21, wherein 1# is a surface close to the object surface 1, is a plane, 2# is a surface close to the image surface 6, and is concave to the object surface 1; the # 4 is a bonding surface of the plano-concave lens 22 and the biconvex lens 23, and is concave towards the image surface 6;9# is the surface of the meniscus lens 41 near the object plane 1, and is also the stop position; 18# is a plane surface of the cover glass 7 on the side close to the image plane 6.
In table 1, the corresponding thickness D of the object plane 1 is 5mm, and the distance between the object plane 1 and the 1# surface of the plano-convex lens 21 is 5mm, which is the working distance of the objective lens of this embodiment; the 1# surface has a corresponding thickness D of 3mm, which means that the distance between the 1# surface and the 2# surface is 3mm, that is, the thickness of the plano-convex lens 21 is 3mm; and so on; the 18# surface has a corresponding thickness D of 2mm, which means that the distance between the 18# surface and the image surface 6 is 2mm.
In this embodiment, the correspondence between the numerical aperture of the object and the different magnifications is set as shown in table 2.
TABLE 2
Multiplying power Object space numerical aperture
0.12
10× 0.14
15× 0.16
20× 0.20
30× 0.30
40× 0.40
In this embodiment, the MTF curves of the objective lens are shown in fig. 2 to 4. At different magnifications, the MTF curves for each field of view are concentrated and near the diffraction limit. Table 3 shows the line pairs of the digital microscope objective lens and the common optical microscope objective lens in this example, and it can be seen that the line pairs of this example are higher, and the imaging contrast is better.
TABLE 3 Table 3
Magnification ratio Common optical microscope (lp/mm) The objective lens (lp/mm) of this example
250 320
20× 800 840
40× 1160 1400
In this embodiment, the point column diagrams of the objective lens are shown in fig. 5 to 7. The dot column diagrams RMS corresponding to different view fields with different multiplying powers are all in a pixel size range, and the resolution meets the requirements of an image sensor.
In this embodiment, the objective lens achieves the following optical indexes:
focal length: f' =5.1 to 60.9mm;
Working distance: 5.2mm;
Distortion: <0.55% (all zoom positions);
resolution ratio: can be matched with an image sensor with the pixel of 350-500 ten thousand pixels under 1/3 inch and 1/3 inch.
Further, it is to be understood that various changes and modifications of the present application may be made by those skilled in the art after reading the above description of the application, and that such equivalents are intended to fall within the scope of the application as defined in the appended claims.

Claims (6)

1. The high-magnification large-zoom-ratio digital microscope objective lens is characterized by comprising a first lens group (2) with positive focal power, a second lens group (3) with positive focal power, a third lens group (4) with negative focal power and a fourth lens group (5) with negative focal power, which are sequentially arranged between an object plane (1) and an image plane (6);
The first lens group (2) is a front fixed group, and the distance between the first lens group and the object plane (1) is fixed; the first lens group (2) is composed of a plano-convex lens (21) having positive optical power, a plano-concave lens (22) having negative optical power, and a first biconvex lens (23) having positive optical power; the plano-concave lens (22) and the first biconvex lens (23) are glued components;
The second lens group (3) is a magnification-varying group, and different magnifications and continuous magnification variation of the magnifications are realized by moving along the optical axis O; the second lens group (3) is composed of a second biconvex lens (31) having positive optical power and a first meniscus lens (32) having negative optical power; the second biconvex lens (31) and the first meniscus lens (32) are glued components; the moving distance of the second lens group (3) is 20-30 mm;
The third lens group (4) is a compensation group, and magnification compensation is carried out on magnification change by moving along the optical axis O; the third lens group (4) is composed of a second meniscus lens (41) having negative optical power, a third meniscus lens (42) having negative optical power; the second meniscus lens (41) and the third meniscus lens (42) are glued components; the moving distance of the third lens group (4) is 70-80 mm;
the fourth lens group (5) is a rear fixed group, and the distance between the fourth lens group and the image plane (6) is fixed; the fourth lens group (5) is composed of a biconcave lens (51) having negative optical power, a fourth meniscus lens (52) having negative optical power, and a third biconvex lens (53) having positive optical power; the fourth meniscus lens (52) and the third biconvex lens (53) are glued components.
2. The high magnification digital microscope objective of claim 1, wherein the magnification is as low as 4× 6×, as high as 32× 48×, and the magnification is up to 8 times or more.
3. The high magnification digital microscope objective of claim 1, wherein the digital microscope objective meets the following conditions:
0.2<|f1/f|<2.2;
0.5<|f2/f|<6.1;
0.8<|f3/f|<8.9;
2.0<|f4/f|<23.3;
wherein f is the total focal length of the digital microscope objective lens, f1 is the focal length of the first lens group (2), f2 is the focal length of the second lens group (3), f3 is the focal length of the third lens group (4), and f4 is the focal length of the fourth lens group (5).
4. The high magnification digital microscope objective according to claim 1, characterized in that the minimum spacing of the first lens group (2) and the second lens group (3) is 5 mm;
the minimum interval between the second lens group (3) and the third lens group (4) is 4 mm.
5. The high magnification ratio digital microscope objective of claim 1, wherein the focal length range of the digital microscope objective is 5-61 mm, and the working distance is 5-15 mm.
6. The high magnification digital microscope objective according to claim 1, further comprising a stop at the second meniscus lens (41) of the third lens group (4), the aperture of the stop remaining unchanged during magnification.
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