CN110527652A - A kind of degradation of formaldehyde, the complex microbial inoculum of phenol mix waste gas and application - Google Patents
A kind of degradation of formaldehyde, the complex microbial inoculum of phenol mix waste gas and application Download PDFInfo
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- CN110527652A CN110527652A CN201910876495.7A CN201910876495A CN110527652A CN 110527652 A CN110527652 A CN 110527652A CN 201910876495 A CN201910876495 A CN 201910876495A CN 110527652 A CN110527652 A CN 110527652A
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- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
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Abstract
The present invention provides a kind of degradation of formaldehyde, the complex microbial inoculum of phenol mix waste gas and applications, by pseudomonas putida, bacillus subtilis, bacillus cereus, Acinetobacter calcoaceticus, streptococcus lactis in proportion mixed processing formaldehyde, phenol mix waste gas when, the 20s time can remove in exhaust gas 90% or more formaldehyde and phenol, reach national emission standard, greatlys improve treatment effeciency.
Description
Technical field
The present invention relates to technical field of waste gas treatment more particularly to the microorganisms of a kind of degradation of formaldehyde, phenol mix waste gas
Composite bacteria agent and application.
Background technique
In chemical production process, formaldehyde, phenol are production primary raw materials, toxic and volatile, in use first
Aldehyde, phenol can volatilize mix waste gas, and the mix waste gas evaporated seriously affects the health of the mankind.
The exhaust-gas treatment mostly uses water to absorb and the method for activated carbon adsorption at present, but that there are treatment effects is undesirable, fortune
Row manages cumbersome, the high problem of operating cost, it is therefore desirable in addition seek approach solution, in view of this, market occurs using biology
Method handles exhaust gas, such as handles exhaust gas using various microbial inoculums, and effect is preferable, but there is a problem of handling time length, leads to efficiency
It is low, it needs to improve.
Summary of the invention
In view of this, one of the objects of the present invention is to provide the microorganisms of a kind of degradation of formaldehyde, phenol mix waste gas to answer
Combined bacteria agent, to solve the above problems.
In order to solve the above technical problems, the technical scheme is that
A kind of complex microbial inoculum of degradation of formaldehyde, phenol mix waste gas,
In advanced optimizing, pseudomonas putida 30%, bacillus subtilis 35%, bacillus cereus 20%, vinegar
Sour calcium acinetobacter calcoaceticus 10%, streptococcus lactis 5%.
In advanced optimizing, pseudomonas putida 20%, bacillus subtilis 30%, bacillus cereus 20%, vinegar
Sour calcium acinetobacter calcoaceticus 15%, streptococcus lactis 15%.
In advanced optimizing, pseudomonas putida 25%, bacillus subtilis 30%, bacillus cereus 17%, vinegar
Sour calcium acinetobacter calcoaceticus 15%, streptococcus lactis 13%.
In advanced optimizing, pseudomonas putida 25%, bacillus subtilis 30%, bacillus cereus 18%, vinegar
Sour calcium acinetobacter calcoaceticus 15%, streptococcus lactis 12%.
In advanced optimizing, pseudomonas putida 25%, bacillus subtilis 30%, bacillus cereus 20%, vinegar
Sour calcium acinetobacter calcoaceticus 20%, streptococcus lactis 5%.
When mentioned microorganism composite bacteria agent handles formaldehyde, phenol mix waste gas in bio-trickling device, the 20s time
The formaldehyde and phenol of 90% or more removal, reach national emission standard, greatly improve treatment effeciency.
The present invention simultaneously provides the preparation method of mentioned microorganism composite bacteria agent, and specific preparation process is as follows:
(1) sample and prepare culture medium
It takes activated sludge as inoculum, sample diluting liquid will be obtained after activated sludge sterile water gradient dilution, it is spare,
Configuration is good using formaldehyde, phenol as the Selective agar medium of carbon source；
(2) strain separating
It takes sample diluting liquid to be uniformly coated on Selective agar medium, training in constant temperature and humidity incubator is inverted into after inoculation
It supports, observation is sampled after 48h；
(3) bacterial strain screening
The single colonie for picking out the different shape that step (2) is isolated is inoculated in respectively on plate Selective agar medium, is inverted
It is put into culture in constant temperature and humidity incubator, the bacterium colony grown after 48h is cultivated and continues selection culture 2 times according to above-mentioned screening technique,
Then inclined-plane scribing line culture is carried out to the single colonie for choosing 5 kinds of preferable different shapes of growing way, and numbers preservation
(4) bacterial strain is identified
Fatty acid identification is carried out to the bacterium colony of preservation, obtains dominant bacteria type；
(5) prepared by composite bacteria agent
5 plants of bacterial strains that step (3) is filtered out expand culture respectively, the high concentration strain cultured solution obtained to culture
High speed refrigerated centrifuge is carried out, centrifuging temperature is 4 DEG C, obtains concentration bacterium solution, and protective agent is added and mixes, and carries out vacuum freeze drying and obtains
To single microbial inoculum, single microbial inoculum is mixed to prepare to the complex microbial inoculum of the processing formaldehyde, phenol according to a certain percentage.
Wherein, Selective agar medium ingredient includes: formaldehyde 6-12mL/L, phenol 6-12mL/L, disodium hydrogen phosphate 2g/L, phosphoric acid
Potassium dihydrogen 1g/L, ammonium sulfate 0.5g/L, epsom salt 0.2g/L, agar 15g/L, microelement, pH 6.8-7.5.
Wherein microelement: FeSO4·7H2O-0.012g/L, MnSO4·7H2O-0.003g/L, ZnSO4·7H2O-
0.003g/L, CoSO4·7H2O-0.001g/L。
Wherein, cultivation temperature described in step (2) and (3) is 30 DEG C, humidity 90%.
Wherein, expansion culture medium used in step (5) are as follows: pancreas peptone soybean broth 30g, agar 15g, distilled water 1L,
Adjusting pH is 7.0.
Wherein, protective agent described in step (5) is 5% glycerol, and additional amount is identical as concentration bacterium solution volume.
Wherein, expand 30 DEG C of cultivation temperature of culture, shaking table speed 150r/min described in step (5), incubation time is
24-48h。
Complex microbial inoculum is prepared by the above method, toxigenic capacity is low, and process is short.
Present invention simultaneously provides application of the mentioned microorganism composite bacteria agent in degradation of formaldehyde, phenol mix waste gas.
Specifically, mentioned microorganism composite bacteria agent is inoculated into the biologic packing material of bio-trickling device, then by first
By bio-trickling device, complex microbial inoculum inoculum concentration is to recycle in bio-trickling device water tank for aldehyde, phenol mix waste gas
The 5%-10% of water quality.
Beneficial effects of the present invention: the present invention mixes pseudomonas putida, bacillus subtilis, waxy according to a certain percentage
Complex microbial inoculum is made in bacillus, Acinetobacter calcoaceticus and streptococcus lactis, can remove formaldehyde, benzene in very short time
Phenol reaches national emission standard.
Specific embodiment
With reference to embodiments, a specific embodiment of the invention is described in further detail, so that technical solution of the present invention
It is more readily understood and grasps.
1, prepared by microbial inoculum
Embodiment 1
Prepare the preparation method of complex microbial inoculum, the specific steps are as follows:
(1) sample and prepare culture medium
It takes activated sludge as inoculum, activated sludge is settled into 30min, the dense bed mud of 50mL is taken to be made respectively with sterile water
Make 10-7、10-8、10-9Gradient dilution liquid, spare, configuration is good using formaldehyde, phenol as the Selective agar medium of carbon source；
The Selective agar medium ingredient are as follows: formaldehyde 12mL/L, phenol 12mL/L, disodium hydrogen phosphate 2g/L, potassium dihydrogen phosphate
1g/L, ammonium sulfate 0.5g/L, epsom salt 0.2g/L, agar 15g/L, microelement: FeSO4·7H2O-0.012g/L,
MnSO4·7H2O-0.003g/L, ZnSO4·7H2O-0.003g/L, CoSO4·7H2O-0.001g/L, pH 6.8-7.5；
(2) strain separating
Take 10-7、10-8、10-9Gradient dilution liquid is drawn 1mL respectively and is uniformly coated on plate Selective agar medium, after inoculation
Dilution applied uniformly with dedicated sterilizing spreading rod, and be inverted into culture in constant temperature and humidity incubator, cultivation temperature is 30 DEG C,
Humidity is sampled observation after being 90%, 48h；
(3) bacterial strain screening
The single colonie for picking out the different shape that step (2) is isolated is inoculated in respectively on plate Selective agar medium, is inverted
It is put into culture in constant temperature and humidity incubator, the bacterium colony grown after 48h is cultivated and continues selection culture 2 times according to above-mentioned screening technique,
Then inclined-plane scribing line culture is carried out to the single colonie for choosing 5 kinds of preferable different shapes of growing way, and numbers preservation；
(4) bacterial strain is identified
Bacterium colony progress fatty acid identification to preservation, the extraction of identification method foundation MIDI fatty acid identification systems offer,
5 plants of bacterial strains of identification method, acquisition are respectively；Pseudomonas putida, bacillus subtilis, bacillus cereus, calcium acetate is not
Lever bacterium, streptococcus lactis.
(5) prepared by composite bacteria agent
4 plants of bacterial strains that step (3) is filtered out expand culture respectively, and 30 DEG C of cultivation temperature, shaking table speed 150r/
Min, incubation time 24-48h carry out high speed refrigerated centrifuge, centrifuging temperature 4 to the high concentration strain cultured solution that culture obtains
DEG C, concentration bacterium solution is obtained, protective agent is added and mixes, vacuum freeze drying is carried out and obtains single microbial inoculum, by single microbial inoculum according to matter
It measures part to calculate, pseudomonas putida 30%, bacillus subtilis 35%, bacillus cereus 20%, Acinetobacter calcoaceticus
10%, streptococcus lactis 5% is mixed to prepare the complex microbial inoculum of degradation of formaldehyde, phenol mix waste gas；
Wherein expand culture medium are as follows: pancreas peptone soybean broth 30g, agar 15g, distilled water 1L, adjusting pH is 7.0；It protects
Protect agent are as follows: 5% glycerol, additional amount are identical as concentration bacterium solution volume.
Embodiment 2
Compared with Example 1, complex microbial inoculum is mixed with by following mass parts, and pseudomonas putida 20% is withered
Careless bacillus 30%, bacillus cereus 20%, Acinetobacter calcoaceticus 15%, streptococcus lactis 15%.
Embodiment 3
Compared with Example 1, complex microbial inoculum is mixed with by following mass parts, and pseudomonas putida 25% is withered
Careless bacillus 30%, bacillus cereus 17%, Acinetobacter calcoaceticus 15%, streptococcus lactis 13%.
Embodiment 4
Compared with Example 1, complex microbial inoculum is mixed with by following mass parts, and pseudomonas putida 25% is withered
Careless bacillus 30%, bacillus cereus 18%, Acinetobacter calcoaceticus 15%, streptococcus lactis 12%.
Embodiment 5
Compared with Example 1, complex microbial inoculum is mixed with by following mass parts, and pseudomonas putida 25% is withered
Careless bacillus 30%, bacillus cereus 20%, Acinetobacter calcoaceticus 20%, streptococcus lactis 5%.
Comparative example 1
The microbial inoculum of single Pseudomonas putida species is prepared by preparation method in embodiment 1.
Comparative example 2
The microbial inoculum of single Bacillus subtilis strain is prepared by preparation method in embodiment 1.
Comparative example 3
The microbial inoculum of single bacillus cereus strain is prepared by preparation method in embodiment 1.
Comparative example 4
The microbial inoculum of single Acinetobacter calcoaceticus strain is prepared by preparation method in embodiment 1.
Comparative example 5
The microbial inoculum of single streptococcus lactis strain is prepared by preparation method in embodiment 1.
2, processing test
The microbial inoculum prepared in one chemical plant field test above-described embodiment of Zhangqiu in Shandong province and comparative example, specifically, point
Not by above-mentioned microbial inoculum inoculation membrane formation into the biologic packing material of bio-trickling device, in this test, bio-trickling device is biology drop
Filter tower, biologic packing material are volcanic rock, and inoculum concentration is 5%, 10% of recycled-water quality in bio-trickling filter water tank, the present embodiment
In, exhaust gas is passed through from bio-trickling filter exhaust gas entrance, and flow 30L/min, concentration of formaldehyde is in 280-320mg.m in exhaust gas-3, benzene
Phenol concentration is in 280-320mg.m-3Between, the temperature of Water in Water Tanks is 30 DEG C, and biology drop is detected during the residence time is 0-25s
The content of filter tower exit exhaust gas formaldehyde, phenol, as shown in table 1- table 6.
Table 1: complex microbial inoculum processing formaldehyde efficiency (residence time 20s, inoculum concentration 10%) in comparative example 1-5
Table 2: complex microbial inoculum processing phenol efficiency (residence time 20s, inoculum concentration 10%) in comparative example 1-5
Table 3: complex microbial inoculum processing formaldehyde efficiency (residence time 0-25s, inoculum concentration 5%) in embodiment 1-5
Table 4: complex microbial inoculum processing phenol efficiency (residence time 0-25s, inoculum concentration 5%) in embodiment 1-5
Table 5: complex microbial inoculum processing formaldehyde efficiency (residence time 0-25s, inoculum concentration 10%) in embodiment 1-5
Table 6: complex microbial inoculum processing phenol efficiency (residence time 0-25s, inoculum concentration 10%) in embodiment 1-5
As can be seen that being inoculated with the bio-trickling filter of this complex microbial inoculum in formaldehyde, dimethylbenzene from above-mentioned table 1-6
Mix waste gas enter after 20s can remove 90% or more exhaust gas, reach national emission standard, when exhaust-gas treatment is greatly reduced
Between, treatment effeciency is improved, is worthy of popularization.
Certainly, the above is only specific application example of the invention, protection scope of the present invention is not limited in any way.It is all
The technical solution formed using equivalent transformation or equivalent replacement is all fallen within rights protection scope of the present invention.
Claims (8)
1. the complex microbial inoculum of a kind of degradation of formaldehyde, phenol mix waste gas, which is characterized in that
2. the complex microbial inoculum of a kind of degradation of formaldehyde according to claim 1, phenol mix waste gas, feature exist
In, pseudomonas putida 30%, bacillus subtilis 35%, bacillus cereus 20%, Acinetobacter calcoaceticus 10%, lactic acid
Streptococcus 5%.
3. the complex microbial inoculum of a kind of degradation of formaldehyde according to claim 1, phenol mix waste gas, feature exist
In, pseudomonas putida 20%, bacillus subtilis 30%, bacillus cereus 20%, Acinetobacter calcoaceticus 15%, lactic acid
Streptococcus 15%.
4. the complex microbial inoculum of a kind of degradation of formaldehyde according to claim 1, phenol mix waste gas, feature exist
In, pseudomonas putida 25%, bacillus subtilis 30%, bacillus cereus 17%, Acinetobacter calcoaceticus 15%, lactic acid
Streptococcus 13%.
5. the complex microbial inoculum of a kind of degradation of formaldehyde according to claim 1, phenol mix waste gas, feature exist
In, pseudomonas putida 25%, bacillus subtilis 30%, bacillus cereus 18%, Acinetobacter calcoaceticus 15%, lactic acid
Streptococcus 12%.
6. the complex microbial inoculum of a kind of degradation of formaldehyde according to claim 1, phenol mix waste gas, feature exist
In, pseudomonas putida 25%, bacillus subtilis 30%, bacillus cereus 20%, Acinetobacter calcoaceticus 20%, lactic acid
Streptococcus 5%.
7. application of the complex microbial inoculum of any of claims 1-6 in degradation of formaldehyde, phenol mix waste gas.
8. application according to claim 7, which is characterized in that the complex microbial inoculum is inoculated into bio-trickling dress
In the biologic packing material set, formaldehyde, phenol mix waste gas are then passed through into bio-trickling device, the complex microbial inoculum inoculation
Amount is the 5%-10% of recycled-water quality in bio-trickling device water tank.
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| CN201910876495.7A CN110527652A (en) | 2019-09-17 | 2019-09-17 | A kind of degradation of formaldehyde, the complex microbial inoculum of phenol mix waste gas and application |
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| CN201910876495.7A CN110527652A (en) | 2019-09-17 | 2019-09-17 | A kind of degradation of formaldehyde, the complex microbial inoculum of phenol mix waste gas and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111378608A (en) * | 2020-05-08 | 2020-07-07 | 青岛光华环保科技有限公司 | Composite microbial inoculum for degrading waste gas of coal chemical sewage station, preparation method and application |
| CN113773995A (en) * | 2021-09-23 | 2021-12-10 | 江苏蓝必盛化工环保股份有限公司 | Formaldehyde efficient degradation microbial inoculum, preparation and application thereof |
| CN114177767A (en) * | 2021-12-06 | 2022-03-15 | 北京北排装备产业有限公司 | Biological deodorization filler and preparation method and application thereof |
| CN115449495A (en) * | 2022-09-22 | 2022-12-09 | 武汉轻工大学 | Complex microbial inoculant and semi-coke wastewater treatment method |
-
2019
- 2019-09-17 CN CN201910876495.7A patent/CN110527652A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111378608A (en) * | 2020-05-08 | 2020-07-07 | 青岛光华环保科技有限公司 | Composite microbial inoculum for degrading waste gas of coal chemical sewage station, preparation method and application |
| CN113773995A (en) * | 2021-09-23 | 2021-12-10 | 江苏蓝必盛化工环保股份有限公司 | Formaldehyde efficient degradation microbial inoculum, preparation and application thereof |
| CN114177767A (en) * | 2021-12-06 | 2022-03-15 | 北京北排装备产业有限公司 | Biological deodorization filler and preparation method and application thereof |
| CN114177767B (en) * | 2021-12-06 | 2024-03-29 | 北京北排装备产业有限公司 | Biological deodorizing filler and preparation method and application thereof |
| CN115449495A (en) * | 2022-09-22 | 2022-12-09 | 武汉轻工大学 | Complex microbial inoculant and semi-coke wastewater treatment method |
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Application publication date: 20191203 |