CN110507814B - Suspension for treating yellow-white dysentery of young deer and using method thereof - Google Patents

Suspension for treating yellow-white dysentery of young deer and using method thereof Download PDF

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CN110507814B
CN110507814B CN201910968391.9A CN201910968391A CN110507814B CN 110507814 B CN110507814 B CN 110507814B CN 201910968391 A CN201910968391 A CN 201910968391A CN 110507814 B CN110507814 B CN 110507814B
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parts
suspension
young deer
white
lactoferrin
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CN110507814A (en
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张雪梅
李海涛
苗利光
刘艳环
桑锐
于逸凡
周鸿缘
王萌
葛冰洁
王政
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Yanbian University
Institute Special Animal and Plant Sciences CAAS
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Yanbian University
Institute Special Animal and Plant Sciences CAAS
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Abstract

A suspension for treating yellow-white dysentery of young deer and a using method thereof belong to the technical field of veterinary medicine. In order to solve the problem that young deer is susceptible to yellow-white dysentery but cannot be effectively treated, the invention provides a suspension for treating yellow-white dysentery of young deer and a using method thereof, wherein the suspension is prepared from the following components: 60 parts of Chinese pulsatilla root, 30 parts of scutellaria baicalensis, 30 parts of golden cypress, 45 parts of coptis chinensis, 60 parts of ash bark, 110 parts of wrinkled gianthyssop herb, 30 parts of radix curcumae, 15 parts of myrobalan, 30 parts of gardenia, 15 parts of white paeony root, 30 parts of liquorice, 40-80 parts of montmorillonite, 40-80 parts of isomaltooligosaccharide, 0.8-1.6 parts of lactoferrin hydrolysate and 2000 parts of water, wherein the lactoferrin white hydrolysate is a product obtained by drying lactoferrin after pepsin hydrolysis until the water content is lower than 6% in mass fraction. The suspension prepared by the invention can effectively treat yellow-white dysentery of the young deer, has simple use method and is suitable for the young deer.

Description

Suspension for treating yellow-white dysentery of young deer and using method thereof
Technical Field
The invention belongs to the technical field of veterinary medicine, and particularly relates to a suspension for treating yellow-white dysentery of a young deer and a using method thereof.
Background
The yellow-white dysentery of the young deer is the yellow dysentery of the young deer and the white dysentery of the young deer caused by the Escherichia coli infection, mainly occurs in the young deer, and the clinical common symptoms are yellow or white loose stools, listlessness, rough hair, dehydration, electrolyte disorder, frequent lying, abdominal pain and borborygmus. In the early stage of disease, the feces are thin and soft, and then the feces are converted into watery stool. The young deer is born in 4-5 months, the influence of environmental factors such as unstable temperature, more rainfall and the like is large, and the morbidity and the mortality are high, so that the young deer is an important reason for diarrhea and death of the young deer. Although clinically, the animal adopts antibiotics, traditional Chinese medicines, probiotics and other methods to prevent and treat yellow-white dysentery of the young deer, the effect is poor.
Disclosure of Invention
The invention provides a suspension for treating yellow-white dysentery of young deer and a using method thereof, aiming at solving the problem that the young deer is susceptible to yellow-white dysentery but cannot be effectively treated. The suspension for treating yellow-white dysentery of the young deer is prepared from the following components:
50-70 parts of Chinese pulsatilla root, 20-40 parts of scutellaria baicalensis, 20-40 parts of golden cypress, 40-50 parts of coptis chinensis, 50-70 parts of ash bark, 100-120 parts of wrinkled gianthyssop herb, 20-40 parts of radix curcumae, 10-20 parts of myrobalan, 20-40 parts of gardenia, 10-20 parts of white paeony root, 20-40 parts of liquorice, 40-80 parts of montmorillonite, 40-80 parts of isomaltooligosaccharide, 0.8-1.6 parts of lactoferrin hydrolysate and 1800-2200 parts of water, wherein the weight ratio of the montmorillonite to the isomaltooligosaccharide is 1:1, the weight ratio of the montmorillonite to the lactoferrin hydrolysate is 50:1, and the lactoferrin hydrolysate is a product obtained by hydrolyzing and drying lactoferrin until the water content is lower than 6% by mass after pepsin.
Preferably, the suspension is prepared from the following components in parts by weight: 60 parts of Chinese pulsatilla root, 30 parts of scutellaria baicalensis, 30 parts of golden cypress, 45 parts of coptis chinensis, 60 parts of ash bark, 110 parts of wrinkled gianthyssop, 30 parts of radix curcumae, 15 parts of myrobalan, 30 parts of cape jasmine, 15 parts of white paeony root, 30 parts of liquorice, 40-80 parts of montmorillonite, 40-80 parts of isomaltooligosaccharide, 0.8-1.6 parts of lactoferrin hydrolysate and 2000 parts of water.
The suspension for treating yellow-white dysentery of the young deer comprises the following preparation steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix with water, decocting, removing residue, concentrating, and concentrating to 1/5 volume before concentration to obtain solution A;
(3) carrying out enzymolysis on lactoferrin by utilizing pepsin under the conditions that the pH is 2.9-3.1 and the temperature is 36-38 ℃, centrifuging the enzymolysis liquid, collecting supernatant, carrying out vacuum freeze drying on the supernatant until the water content mass fraction is lower than 6%, crushing and sieving to obtain a lactoferrin hydrolysate;
(4) mixing montmorillonite and isomaltooligosaccharide, pulverizing, and sieving to obtain mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with the mixture of the montmorillonite and the isomaltooligosaccharide obtained in the step (4), wherein the mixture is powder B;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: (1-2) g of the above raw materials are mixed together to obtain a suspension for preventing and treating yellow and white dysentery of the young deer.
Preferably, the decocting temperature in the step (2) is 110-115 ℃, the pressure is 0.1Mpa, and the time is 30 min.
Preferably, the concentration in the step (2) is performed by reduced pressure distillation, the conditions are 55-70 ℃ water bath, and the rotating speed is 60-100 rpm.
Preferably, the lactoferrin enzymolysis in the step (3) is to select a lactoferrin solution with the mass concentration of 6% -8%, the ratio of the pepsin activity to the lactoferrin solution is 1.00KU/g, the pH is 3.0, the lactoferrin solution is hydrolyzed for 4 hours under the condition of 37 ℃ water bath, after the reaction is finished, the enzyme is inactivated at 75 ℃ for 15 minutes, the pH is adjusted to 4.5, and the lactoferrin solution is centrifuged for 15 minutes at 12000g at 4 ℃ to obtain a supernatant.
Preferably, the vacuum freeze-drying condition in the step (3) is-80 to-90 ℃, the vacuum degree is 0.0010Mpa, and the freeze-drying is carried out for 24 hours.
Preferably, the crushing, sieving and selecting a 400-mesh sieve in the step (3).
Preferably, the crushing and sieving in the step (4) are performed by selecting a 400-mesh sieve.
The application method of the suspension for preventing and treating yellow-white dysentery of the young deer, prepared by the invention, is as follows: the suspension for preventing and treating yellow-white dysentery of young deer is orally taken or mixed with milk and feed for use 1 time a day according to 50 mL/time of each young deer, and is continuously taken for 5-7 days.
Advantageous effects
The oral suspension for treating yellow and white scour of newborn deer contained in the invention has the following functions:
pulsatillae radix, the rhizome of Pulsatilla chinensis (Bunge) Regel, a perennial herb of the genus anemone, family Ranunculaceae; bitter and cold in nature; entering stomach and large intestine meridians; has effects of clearing away heat and toxic materials, cooling blood, relieving dysentery, eliminating dampness, and killing parasite.
Scutellaria baicalensis, root of Scutellaria baicalensis Georgi, a perennial herb of the genus Scutellaria of the family Labiatae; bitter taste and cold nature, and has effects of clearing heat, eliminating dampness, clearing pathogenic fire, removing toxic substance, stopping bleeding, preventing miscarriage, etc.; it can be used for treating epidemic febrile disease, upper respiratory infection, cough due to lung heat, damp-heat yellow gallbladder, pneumonia, dysentery, hemoptysis, conjunctival congestion, fetal irritability, hypertension, carbuncle, furuncle, and sore.
Phellodendron amurense, a dried bark of Phellodendron chinense schneid, a plant of the rutaceae family; light smell, extremely bitter taste, stickiness when chewed, cold in nature, entering kidney and bladder meridians; has effects of clearing heat, eliminating dampness, purging pathogenic fire, removing toxic substance, and treating sore, and can be used for treating dysentery due to damp-heat, jaundice, dark urine, pruritus vulvae, stranguria with pain, tinea pedis, atrophic debility cramped, bone steaming, fatigue, night sweat, nocturnal emission, pyocutaneous disease, toxic swelling, eczema, and eczema.
Coptidis rhizoma, dried rhizome of Coptis chinensis Franch, a perennial herb of Coptis chinensis Franch of the family Ranunculaceae; bitter taste and cold nature, and enters heart, spleen, stomach, liver, gallbladder and large intestine; clearing heat and eliminating dampness, purging pathogenic fire and removing toxic substances, and can be used for treating damp-heat, abdominal fullness, emesis, acid regurgitation, dysentery, jaundice, hyperpyrexia, coma, heart fire hyperactivity, vexation, insomnia, palpitation, hematemesis, epistaxis, conjunctival congestion, toothache, diabetes, carbuncle, swelling, and sore; it is used externally to treat eczema, eczema and purulent ear canal.
Cortex Fraxini, dry branch bark or dry bark of Fraxinus rhynchophylla Hance, Fraxinus pinnata or Fraxinus pinnitafida of Oleaceae; bitter and astringent taste, cold in nature, entering liver, gallbladder and large intestine meridians; mainly used for clearing heat and drying dampness, astringing and stopping dysentery, stopping leukorrhagia and improving eyesight.
Agastache rugosa, aerial parts of Pogostemmincablin (blanco) Benth, a herb of the family Labiatae; pungent in flavor and warm in nature, and enter spleen, stomach and lung meridians; resolving dampness and enlivening the spleen, keeping the evil spirits and regulating the middle warmer, relieving summer-heat, relieving exterior syndrome, and can be used for treating damp obstruction of spleen and stomach, abdominal distention, early damp-warm syndrome, emesis, diarrhea, summer-heat dampness, fever with aversion to cold, fullness and oppression in chest and stomach, etc.
Radix Curcumae, dried root tuber of Curcuma wenyujin Y, H.Chenet C.Ling of Zingiberaceae; pungent, bitter and cold in flavor, entering liver, heart and lung meridians; promoting blood circulation, relieving pain, activating qi-flowing, resolving stagnation, clearing heart fire, cooling blood, promoting bile flow, and removing jaundice, and can be used for treating stabbing pain in chest and hypochondrium, thoracic obstruction, cardialgia, amenorrhea, dysmenorrhea, breast pain, fever unconsciousness, epilepsy, hematemesis, jaundice, and dark urine.
Myrobalan, dried ripe fruit of myrobalan Terminalia chebula retz, or myrobalan Terminalia chebula retz, var. tomatella kurt, family quisqualaceae; bitter, sour, astringent and neutral in nature; entering lung and large intestine meridians; astringe intestines to check diarrhea, astringe lung to stop cough, lower fire to relieve sore throat, and can be used for treating chronic diarrhea and dysentery, hematochezia, rectocele, lung deficiency, cough and asthma, chronic cough, pharyngalgia, hoarseness.
Gardenia jasminoides Ellis, fruit of Gardenia jasminoides Ellis of Rubiaceae; bitter and cold meridians entering heart, lung and triple energizer meridians; clearing heat, purging fire, cooling blood, and can be used for treating fever, vexation, insomnia, jaundice, gonorrhea, diabetes, conjunctival congestion, pharyngalgia, hematemesis, epistaxis, bloody dysentery, hematuria, and pyocutaneous disease due to heat toxin.
White peony root, dried root of Paeonia tactra pall. of Ranunculaceae; bitter and sour in taste, slightly cold in nature; entering liver and spleen meridians; nourishing blood, regulating menstruation, astringing yin, arresting sweating, softening liver, relieving pain, and suppressing liver yang, and can be used for treating blood deficiency, sallow complexion, menoxenia, spontaneous perspiration, night sweat, hypochondriac pain, abdominal pain, limb spasm pain, headache, and giddiness.
Dried root and rhizome of Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, or Glycyrrhiza glabra L, which are leguminous plants; sweet in taste, neutral in nature, and entering heart, lung, spleen and stomach meridians; has the effects of tonifying spleen and qi, clearing away heat and toxic materials, eliminating phlegm and stopping cough, relieving spasm and pain, and harmonizing the drugs, and is commonly used for treating weakness of spleen and stomach, lassitude and hypodynamia, palpitation and shortness of breath, cough with excessive phlegm, abdominal and limb spasm and pain, carbuncle, swelling and sore toxicity, and relieving drug toxicity and strong property.
Montmorillonite, the layered mineral Montmorillonite that the aqueous aluminosilicate forms, have lamellar structure and heterogeneous charge distribution, have fixing, inhibiting effects to virus, germ and toxin produced in the alimentary canal; has the capability of covering the mucosa of the digestive tract, and can repair and improve the defense function of the mucosal barrier to the attack factors from the aspects of quality and quantity by combining with the mucus glycoprotein.
Isomaltose hypgather, difficult to be digested by stomach enzyme, the sweetness is low, the calorie is low, basically does not increase blood sugar and blood fat, can not be hydrolyzed at the upper part of the gastrointestinal tract, can not be absorbed by the small intestine after entering the intestinal tract, but directly enters the large intestine, can stimulate the activity or growth and reproduction of bifidobacteria by being selectively utilized by bifidobacteria in the intestinal tract, and can not be utilized by other harmful bacteria in the intestinal tract, thereby inhibiting the growth of harmful bacteria, inhibiting the formation of harmful bacteria and putrefactive substances in the intestinal tract, increasing the vitamin content, improving the immunity of the organism and promoting the adjustment of the microecology in the intestinal tract to a benign cycle.
Lactoferrin (LF) is an important non-heme iron-binding glycoprotein in milk, a monomeric glycoprotein with bactericidal activity in neutrophil granules. It has a molecular weight of 80kDa and is mainly expressed and secreted by mammary epithelial cells. Lactoferrin not only participates in the transport of iron, but also has strong biological functions of broad-spectrum antibiosis, antioxidation, anticancer, immune system regulation and the like, and is considered as a novel antibacterial and anticancer drug and a food and feed additive with great development potential. The lactoferrin hydrolysate is lactoferrin hydrolyzed by pepsin, and can release bioactive peptides with physiological and pharmacological effects, including antibacterial peptides. Therefore, the lactoferrin hydrolysate has great application potential in the aspects of researching novel food preservatives, novel antibiotics, feed additives and the like.
The oral suspension for treating yellow and white scour of newborn deer comprises concentrated solution obtained by mixing and decocting Chinese pulsatilla root, baical skullcap root, amur corktree bark, golden thread, ash bark, wrinkled gianthyssop herb, turmeric root-tuber, myrobalan, gardenia, white paeony root and liquoric root, and has the effects of clearing heat, eliminating dampness, resisting bacteria and diminishing inflammation; in addition, the isomaltooligosaccharide containing the bifidus factor has obvious effect on promoting the growth of bifidobacteria in the large intestine, inhibiting the propagation and growth of harmful bacteria and regulating the intestinal microecological balance.
The oral suspension for treating yellow-white dysentery of young deer is prepared by uniformly mixing a traditional Chinese medicine decoction concentrated solution (A solution) with an anti-diarrhea function and solid powder montmorillonite, isomaltooligosaccharide and lactoferrin hydrolysate (B powder) before use to form a suspension, and the oral suspension is orally taken or drenched.
The oral suspension for treating yellow and white scour of newborn deer comprises lactoferrin hydrolysate, can release bioactive peptide with antibacterial activity after being hydrolyzed by pepsin, and has good antibacterial effect on gram-negative bacteria escherichia coli.
Detailed Description
The present invention will be further described with reference to the following specific examples, but the present invention is not limited to these examples.
Example 1 suspension for the prevention and treatment of yellow-white dysentery of young deer.
The suspension for preventing and treating yellow-white dysentery of the young deer is prepared from the following components in parts by weight: 50 parts of Chinese pulsatilla root, 40 parts of scutellaria baicalensis, 20 parts of golden cypress, 50 parts of coptis chinensis, 50 parts of ash bark, 120 parts of wrinkled gianthyssop, 20-parts of radix curcumae, 20 parts of myrobalan, 20 parts of cape jasmine, 20 parts of white paeony root, 20 parts of liquorice, 40 parts of montmorillonite, 40 parts of isomaltooligosaccharide, 0.8 part of lactoferrin hydrolysate and 1800 parts of water.
The suspension for preventing and treating yellow and white dysentery of the young deer, which is described in the embodiment, is prepared according to the following steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix and water, and decocting at 110 deg.C under 0.1Mpa for 30 min. Removing residues, transferring into rotary evaporator, and distilling under reduced pressure in water bath at 55 deg.C and rotation speed of 60rpm until the volume of solution is 1/5 before concentration, to obtain solution A;
(3) the lactoferrin (purity is more than 95%) has a substrate concentration of 6% (w/v), pH of 2.9 and a ratio (E/S) of pepsin activity to lactoferrin concentration of 1.00KU/g, and is hydrolyzed in a water bath at 37 ℃ for 4 hours. Inactivating enzyme at 75 deg.C for 15min after reaction, adjusting pH to 4.5, centrifuging at 4 deg.C for 15min at 12000g, collecting supernatant, and lyophilizing at-86 deg.C under vacuum degree of 0.0010Mpa for 24 h. After drying, collecting lactoferrin hydrolysate, performing moisture determination, and crushing and sieving with a 400-mesh sieve to obtain lactoferrin hydrolysate, wherein the water content is 5.72%;
(4) mixing montmorillonite and isomaltooligosaccharide, crushing and sieving with a 400-mesh sieve to obtain a mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with a mixture of montmorillonite and isomalto-oligosaccharide to obtain powder B;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: 1g of the mixture is mixed to obtain the suspension for preventing and treating yellow and white scour of the young deer.
The present invention will be further described with reference to the following specific examples, but the present invention is not limited to these examples.
Example 2 suspension for the prevention and treatment of yellow-white dysentery of young deer.
The suspension for preventing and treating yellow-white dysentery of the young deer is prepared from the following components in parts by weight: 70 parts of Chinese pulsatilla root, 20 parts of scutellaria baicalensis, 40 parts of golden cypress, 40 parts of coptis chinensis, 70 parts of ash bark, 100 parts of wrinkled gianthyssop, 40 parts of radix curcumae, 10 parts of myrobalan, 40 parts of gardenia, 10 parts of white paeony root, 40 parts of liquorice, 60 parts of montmorillonite, 60 parts of isomaltooligosaccharide, 1.2 parts of lactoferrin hydrolysate and 1900 parts of water.
The suspension for preventing and treating yellow-white dysentery of the young deer, which is described in the embodiment, is prepared according to the following steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix and water, and decocting at 113 deg.C under 0.1Mpa for 30 min. Removing residues, transferring into rotary evaporator, and distilling under reduced pressure in water bath at 60 deg.C and rotation speed of 100rpm until the volume of solution is 1/5 before concentration, to obtain solution A;
(3) the lactoferrin (purity is more than 95%) has substrate concentration of 6% (w/v), pH of 3.0, and the ratio of pepsin activity to lactoferrin concentration (E/S) of 1.00KU/g, and is hydrolyzed in water bath at 37 deg.C for 4 h. Inactivating enzyme at 75 deg.C for 15min after reaction, adjusting pH to 4.5, centrifuging at 4 deg.C for 15min at 12000g, collecting supernatant, and freeze drying at-80 deg.C under 0.0010Mpa for 24 hr. After drying, collecting the lactoferrin hydrolysate, performing moisture determination, and crushing and sieving with a 400-mesh sieve to obtain the lactoferrin hydrolysate, wherein the water content is 5.69%;
(4) mixing montmorillonite and isomaltooligosaccharide, crushing and sieving with a 400-mesh sieve to obtain a mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with a mixture of montmorillonite and isomaltooligosaccharide, wherein the mixture is powder B;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: 1.5g of the mixture is mixed to obtain the suspension for preventing and treating yellow and white scour of the young deer.
Example 3 suspension for the prevention and treatment of yellow-white dysentery of young deer.
The suspension for preventing and treating yellow and white dysentery of the young deer is prepared from the following components in parts by weight: 55 parts of Chinese pulsatilla root, 35 parts of scutellaria baicalensis, 25 parts of golden cypress, 45 parts of coptis chinensis, 55 parts of ash bark, 115 parts of wrinkled gianthyssop, 25 parts of radix curcumae, 18 parts of myrobalan, 30 parts of cape jasmine, 15 parts of white paeony root, 25 parts of liquorice, 50 parts of montmorillonite, 50 parts of isomaltooligosaccharide, 1 part of lactoferrin hydrolysate and 2000 parts of water.
The suspension for preventing and treating yellow-white dysentery of the young deer, which is described in the embodiment, is prepared according to the following steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix and water, and decocting at 115 deg.C under 0.1Mpa for 30 min. Removing residues, transferring into rotary evaporator, and distilling under reduced pressure in water bath at 70 deg.C and rotation speed of 60rpm until the volume of solution is 1/5 before concentration, to obtain solution A;
(3) the lactoferrin (purity is more than 95%) has substrate concentration of 6% (w/v), pH of 3.1, and the ratio of pepsin activity to lactoferrin concentration (E/S) of 1.00KU/g, and is hydrolyzed in water bath at 37 deg.C for 4 h. Inactivating enzyme at 75 deg.C for 15min after reaction, adjusting pH to 4.5, centrifuging at 4 deg.C for 15min at 12000g, collecting supernatant, and freeze drying at-84 deg.C under vacuum degree of 0.0010Mpa for 24 hr. After drying, collecting lactoferrin hydrolysate, performing moisture determination, and crushing and sieving with a 400-mesh sieve to obtain lactoferrin hydrolysate, wherein the water content is 5.75%;
(4) mixing montmorillonite and isomaltooligosaccharide, crushing and sieving with a 400-mesh sieve to obtain a mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with a mixture of montmorillonite and isomaltooligosaccharide, wherein the mixture is powder B;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: 2g of the mixture is mixed to obtain the suspension for preventing and treating yellow and white scour of the young deer.
Example 4 suspension for prevention and treatment of yellow-white dysentery of young deer.
The suspension for preventing and treating yellow and white dysentery of the young deer is prepared from the following components in parts by weight: 65 parts of Chinese pulsatilla root, 25 parts of baical skullcap root, 35 parts of amur corktree bark, 47 parts of golden thread, 65 parts of ash bark, 110 parts of wrinkled gianthyssop herb, 35 parts of turmeric root-tuber, 18 parts of myrobalan, 30 parts of cape jasmine fruit, 16 parts of white paeony root, 35 parts of liquoric root, 70 parts of montmorillonite, 70 parts of isomaltooligosaccharide, 1.4 parts of lactoferrin hydrolysate and 2100 parts of water.
The suspension for preventing and treating yellow and white dysentery of the young deer, which is described in the embodiment, is prepared according to the following steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix and water, and decocting at 110 deg.C under 0.1Mpa for 30 min. Removing residues, transferring into rotary evaporator, and distilling under reduced pressure in water bath at 65 deg.C and rotation speed of 80rpm until the volume of solution is 1/5 before concentration, to obtain solution A;
(3) the lactoferrin (purity is more than 95%) has a substrate concentration of 6% (w/v), pH of 2.9 and a ratio (E/S) of pepsin activity to lactoferrin concentration of 1.00KU/g, and is hydrolyzed in a water bath at 37 ℃ for 4 hours. Inactivating enzyme at 75 deg.C for 15min after reaction, adjusting pH to 4.5, centrifuging at 4 deg.C for 15min at 12000g, collecting supernatant, and freeze drying at-90 deg.C under 0.0010Mpa for 24 h. After drying, collecting lactoferrin hydrolysate, performing moisture determination, and crushing and sieving with a 400-mesh sieve to obtain lactoferrin hydrolysate, wherein the water content is 5.64%;
(4) mixing montmorillonite and isomaltooligosaccharide, crushing and sieving with a 400-mesh sieve to obtain a mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with a mixture of montmorillonite and isomalto-oligosaccharide to obtain powder B;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: 1.2g of the mixture is mixed to obtain the suspension for preventing and treating yellow and white dysentery of the young deer.
Example 5 suspension for prevention and treatment of yellow-white dysentery of young deer.
The suspension for preventing and treating yellow-white dysentery of the young deer is prepared from the following components in parts by weight: 68 parts of Chinese pulsatilla root, 38 parts of baical skullcap root, 22 parts of amur corktree bark, 46 parts of golden thread, 63 parts of ash bark, 106 parts of wrinkled gianthyssop herb, 27 parts of turmeric root-tuber, 12 parts of myrobalan fruit, 25 parts of cape jasmine fruit, 17 parts of white paeony root, 28 parts of liquoric root, 50 parts of montmorillonite, 50 parts of isomaltooligosaccharide, 1 part of lactoferrin hydrolysate and 2200 parts of water.
The suspension for preventing and treating yellow-white dysentery of the young deer, which is described in the embodiment, is prepared according to the following steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix and water, and decocting at 112 deg.C under 0.1Mpa for 30 min. Removing residues, transferring into rotary evaporator, and distilling under reduced pressure in water bath at 60 deg.C and rotation speed of 70rpm until the volume of solution is 1/5 before concentration, to obtain solution A;
(3) the lactoferrin (purity is more than 95%) has a substrate concentration of 6% (w/v), a pH value of 3.0 and a ratio (E/S) of pepsin activity to lactoferrin concentration of 1.00KU/g, and is hydrolyzed for 4 hours under the condition of 37 ℃ water bath. Inactivating enzyme at 75 deg.C for 15min after reaction, adjusting pH to 4.5, centrifuging at 4 deg.C for 15min at 12000g, collecting supernatant, and freeze drying at-80 deg.C under 0.0010Mpa for 24 hr. After drying, collecting lactoferrin hydrolysate, performing moisture determination, and crushing and sieving with a 400-mesh sieve to obtain lactoferrin hydrolysate, wherein the water content is 5.70%;
(4) mixing montmorillonite and isomaltooligosaccharide, crushing and sieving with a 400-mesh sieve to obtain a mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with a mixture of montmorillonite and isomalto-oligosaccharide to obtain powder B;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: 1.8g of the mixture is mixed to obtain the suspension for preventing and treating yellow and white scour of the young deer.
Example 6 suspension for prevention and treatment of yellow-white dysentery of young deer.
The suspension for preventing and treating yellow-white dysentery of the young deer is prepared from the following components in parts by weight: 60 parts of Chinese pulsatilla root, 30 parts of scutellaria baicalensis, 30 parts of golden cypress, 45 parts of coptis chinensis, 60 parts of ash bark, 110 parts of wrinkled gianthyssop, 30 parts of radix curcumae, 15 parts of myrobalan, 30 parts of cape jasmine, 15 parts of white paeony root, 30 parts of liquorice, 80 parts of montmorillonite, 80 parts of isomaltooligosaccharide, 1.6 parts of lactoferrin hydrolysate and 2000 parts of water.
The suspension for preventing and treating yellow-white dysentery of the young deer, which is described in the embodiment, is prepared according to the following steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix and water, and decocting at 110 deg.C under 0.1Mpa for 30 min. Removing the residue, transferring into rotary evaporator, and distilling under reduced pressure in water bath at 55 deg.C and rotation speed of 60rpm until the volume of the solution is 1/5, wherein the concentrated solution is solution A;
(3) the lactoferrin (purity is more than 95%) has substrate concentration of 6% (w/v), pH of 3.1, and the ratio of pepsin activity to lactoferrin concentration (E/S) of 1.00KU/g, and is hydrolyzed in water bath at 37 deg.C for 4 h. Inactivating enzyme at 75 deg.C for 15min after reaction, adjusting pH to 4.5, centrifuging at 4 deg.C for 15min at 12000g, collecting supernatant, and freeze drying at-86 deg.C under vacuum degree of 0.0010Mpa for 24 h. After drying, collecting lactoferrin hydrolysate, performing moisture determination, and crushing and sieving with a 400-mesh sieve to obtain lactoferrin hydrolysate, wherein the water content is 5.83%;
(4) mixing montmorillonite and isomaltooligosaccharide, crushing and sieving with a 400-mesh sieve to obtain a mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with a mixture of montmorillonite and isomalto-oligosaccharide to obtain powder B;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: 1.6g of the mixture is mixed to obtain the suspension for preventing and treating yellow and white scour of the young deer.
In 2019, in 6-7 months, clinical tests for treating yellow-white dysentery of young deer are carried out by Changsheng Jiashenghong breed Cervidae technology Limited company, Changsheng deer industry Limited company and Jusheng deer farm in the village of Liang district of Changchun city, and 70 heads of young deer with yellow-white dysentery symptoms are selected and divided into 6 groups of test groups and 1 group of control groups according to 10 heads of each group. The control group was administered 2 times a day by intramuscular injection of gentamicin sulfate injection (0.2g/5mL, Hebei Yuanzheng pharmaceutical Co., Ltd.) at a weight of 0.24 mg/Kg. The oral suspension for treating yellow-white dysentery of young deer prepared in the experimental groups according to examples 1-6 is orally taken 1 time a day for 5 days by 50 mL/time per young deer. The clinical symptoms and the treatment effect of the animals are observed at the end of the test, and the observation indexes comprise mental state, stool form, effective rate and cure rate. Mental state, good 5 points, better 4 points, generally 3 points, poor 2 points, poor 1 point; feces shape, normal 5 points, softer but not containing mucus or blood 4 points, thin 3 points, water sample thin feces 2 points, water sample thin feces containing mucus or blood 1 point. The total effective rate is the ratio of the number of heads with improved diarrhea symptoms to the total number of heads in an experimental group after the suspension is used. The cure rate is the ratio of the number of cured heads of diarrhea after using the suspension of the invention to the total number of heads of an experimental group.
TABLE 1 therapeutic effect on yellow and white dysentery of young deer in different examples
Figure BDA0002231267040000071
Figure BDA0002231267040000081
The results are shown in table 1, compared with the control group, the treatment effect difference is obvious (P is less than 0.05), the cure rate and the total effective rate of the test group are respectively 88.3 percent and 75.0 percent, and are respectively 18.3 percent and 45.0 percent higher than those of the control group. The oral suspension for treating yellow-white dysentery of young deer has better treatment effect on yellow-white dysentery of young deer than gentamycin sulfate control group.
Although the present invention has been described with respect to the preferred embodiments, it should be understood that they are not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

Claims (8)

1. The preparation method of the suspension for preventing and treating yellow-white dysentery of the young deer is characterized in that the suspension is prepared from the following components in parts by weight: 50-70 parts of Chinese pulsatilla root, 20-40 parts of scutellaria baicalensis, 20-40 parts of golden cypress, 40-50 parts of coptis chinensis, 50-70 parts of ash bark, 100-120 parts of wrinkled gianthyssop herb, 20-40 parts of radix curcumae, 10-20 parts of myrobalan, 20-40 parts of gardenia, 10-20 parts of white paeony root, 20-40 parts of liquorice, 40-80 parts of montmorillonite, 40-80 parts of isomaltooligosaccharide, 0.8-1.6 parts of lactoferrin hydrolysate and 1800-2200 parts of water, wherein the weight ratio of the montmorillonite to the isomaltooligosaccharide is 1:1, the weight ratio of the montmorillonite to the lactoferrin hydrolysate is 50:1, and the lactoferrin hydrolysate is a product obtained by hydrolyzing and drying lactoferrin until the water content is lower than 6% by mass percent after pepsin; the preparation method comprises the following steps:
(1) weighing the components;
(2) mixing radix Pulsatillae, Scutellariae radix, cortex Phellodendri, Coptidis rhizoma, cortex Fraxini, herba Agastaches, radix Curcumae, fructus Chebulae, fructus Gardeniae, radix Paeoniae alba, Glycyrrhrizae radix with water, decocting, removing residue, concentrating, and concentrating to 1/5 volume before concentration to obtain solution A;
(3) hydrolyzing with pepsin at the pH of 3.0 and the temperature of 37 ℃ for 4h to carry out enzymolysis on a lactoferrin solution with the mass concentration of 6-8%, inactivating the enzyme at 75 ℃ for 15min after the reaction is finished, adjusting the pH to 4.5, centrifuging at the temperature of 4 ℃ for 15min at 12000g, collecting the supernatant, carrying out vacuum freeze drying on the supernatant until the water content mass fraction is lower than 6%, crushing and sieving to obtain a lactoferrin hydrolysate; the ratio of the activity of the pepsin to the lactoferrin solution is 1.00 KU/g;
(4) mixing montmorillonite and isomaltooligosaccharide, pulverizing, and sieving to obtain mixture of montmorillonite and isomaltooligosaccharide;
(5) mixing the lactoferrin hydrolysate with the mixture of montmorillonite and isomaltose hypgather obtained in the step (4), wherein the mixture is B powder;
(6) mixing the solution A and the powder B according to the proportion of 5 ml: (1-2) g of the above raw materials are mixed together to obtain a suspension for preventing and treating yellow and white dysentery of the young deer.
2. The preparation method of the suspension for preventing and treating yellow and white dysentery of the young deer as claimed in claim 1, wherein the suspension is prepared from the following components in parts by weight: 60 parts of Chinese pulsatilla root, 30 parts of scutellaria baicalensis, 30 parts of golden cypress, 45 parts of coptis chinensis, 60 parts of ash bark, 110 parts of wrinkled gianthyssop, 30 parts of radix curcumae, 15 parts of myrobalan, 30 parts of cape jasmine, 15 parts of white paeony root, 30 parts of liquorice, 40-80 parts of montmorillonite, 40-80 parts of isomaltooligosaccharide, 0.8-1.6 parts of lactoferrin hydrolysate and 2000 parts of water.
3. The preparation method of the suspension for preventing and treating yellow-white dysentery of young deer as claimed in claim 1, wherein the decocting temperature in step (2) is 110-115 deg.C, the pressure is 0.1Mpa, and the time is 30 min.
4. The preparation method of the suspension for preventing and treating yellow and white scour of the young deer as claimed in claim 1, wherein the concentration in the step (2) is performed by reduced pressure distillation in a water bath at 55-70 ℃ and at a rotation speed of 60-100 rpm.
5. The preparation method of the suspension for preventing and treating yellow and white scour of the young deer as claimed in claim 1, wherein the vacuum freeze-drying conditions in the step (3) are-80 ℃ to-90 ℃, the vacuum degree is 0.0010Mpa, and the freeze-drying is carried out for 24 hours.
6. The preparation method of the suspension for preventing and treating yellow-white dysentery of young deer as claimed in claim 1, wherein the step (3) comprises pulverizing, sieving and selecting 400 mesh sieve.
7. The preparation method of the suspension for preventing and treating yellow-white dysentery of young deer as claimed in claim 1, wherein the step (4) comprises pulverizing, sieving and selecting 400 mesh sieve.
8. The application of the suspension obtained by the preparation method of any one of claims 1-7 in preparing a medicine for preventing and treating yellow and white dysentery of young deer, wherein the suspension in the medicine is orally taken or mixed with milk and feed for 5-7 days according to the dosage of 50 mL/time per young deer for 1 time a day.
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