CN110506103A - System and method for growing algae - Google Patents

System and method for growing algae Download PDF

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Publication number
CN110506103A
CN110506103A CN201880016813.6A CN201880016813A CN110506103A CN 110506103 A CN110506103 A CN 110506103A CN 201880016813 A CN201880016813 A CN 201880016813A CN 110506103 A CN110506103 A CN 110506103A
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Prior art keywords
injector
flow velocity
container
operation flow
algae
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CN201880016813.6A
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Inventor
奥迪德·巴尚
奥哈德·巴尚
史蒂芬·德拉姆梅
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Algae Innovation Co Ltd
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Algae Innovation Co Ltd
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Publication of CN110506103A publication Critical patent/CN110506103A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q3/00—Condition responsive control processes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G33/00—Cultivation of seaweed or algae
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/2319—Methods of introducing gases into liquid media
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
    • B01F33/406—Mixers using gas or liquid agitation, e.g. with air supply tubes in receptacles with gas supply only at the bottom
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/21—Measuring
    • B01F35/211—Measuring of the operational parameters
    • B01F35/2113—Pressure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/21—Measuring
    • B01F35/211—Measuring of the operational parameters
    • B01F35/2115—Temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/21—Measuring
    • B01F35/2132—Concentration, pH, pOH, p(ION) or oxygen-demand
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/22—Control or regulation
    • B01F35/2201—Control or regulation characterised by the type of control technique used
    • B01F35/2202—Controlling the mixing process by feed-back, i.e. a measured parameter of the mixture is measured, compared with the set-value and the feed values are corrected
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
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    • C12M1/00—Apparatus for enzymology or microbiology
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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Abstract

Aspect of the invention is related to spraying the system and method for algae culture container.The method may include control at least one first injector so that first fluid to be assigned in container in the first operation flow velocity；And at least one second injector is controlled so that second fluid to be assigned in container in the second operation flow velocity.First operation flow velocity may be adapted to allow to mix algae in culture vessel, and the second operation flow velocity may be adapted to the assimilation for allowing the material in the liquid in culture vessel.

Description

System and method for growing algae
Invention field
Present invention relates in general to algal growns.More particularly, the present invention relate to enhance the system of algal grown and Method.
Background of invention
In recent years, become using algae culture of the bioreactor (for example, utilizing bubble tower) under artificial condition It is more and more common, such as to produce biomass.For the growth of optimum condition and acceleration, algae (or microalgae) is supplied with richness Containing CO2Air bubble and illumination (artificial light or come from sunlight).About 50% algal biomass is carbon, by photosynthetic solid Determine CO2It obtains, wherein carbon dioxide is needed with liquid phase dissolved in culture.In phototropism algae culturing system, for giving birth to Long main input (or magnanimity nutrients (macro-nutrients)) is light, CO2, nutrients (nitrogen, phosphorus etc.), and Water with turbulent closure scheme, to give these resource allocations to single algae culture cell.
Furthermore, it is necessary to which good fluid is mixed for realizing high algae concentration in the bioreactor.Good mixing can To control the exposure of cell by reducing the degree mutually covered and minimizing Xanthophyll cycle.Effective mixing can be such that cell moves It is dynamic to be inputted close to illuminated surface with obtaining photon, and then far from it, to give before cell is again exposed to light The cell for giving photon saturation absorbs this luminous energy for photosynthetic chance.Since the cell concentration of superelevation is needed using strong Light source, therefore insufficient mixing may cause and be excessively exposed to strong light, and cell may also be caused to damage due to Xanthophyll cycle Wound.
Gas injection (is mainly rich in CO2Air or nitrogen) be commonly used in light-bioreactor (PBR), to produce Raw required mixing.The ascending motion generation and the tangent mixing in flow direction of bubble.Effective mixing usually requires continuous High flow rate and air pocket.However, using jet-stream wind for mixing and its composition being made to be enriched with CO2With intrinsic inefficient Rate, because of CO2It is introduced in air pocket (needed for mixing) with diluted concentration, therefore leads to the CO of about 10% difference2Biology Utilization rate (wherein about 90% CO2It is discharged from bioreactor).
Microalgae can be grown to illumination in the system such as Flat photobioreactor of many types.For algal grown Light source can be any kind of visible light in the range of about 400nm-700nm wavelength.Light emitting diode (LED) has Specific wavelength, such as the ability of the light in visible light (for example, blue and/or red) wave-length coverage are provided.
However, it is some input become it is limited (for example, due to algae from cover caused by restricted light), and cause to Determine the maximal density of the determination of algae in system.If every other input is supplied with non-limiting availability, with algae The density of culture increases, and cell covers the cell being blocked in optical path.Finally, light cannot penetrate into culture far enough In, to allow more growths, and system reaches its maximum (light is limited) concentration.
Summary of the invention
Some aspects of the invention can be related to spraying the method for algae culture container.This method may include controlling at least First fluid to be assigned in container by one the first injector in the first operation flow velocity；And control at least one second injection Second fluid to be assigned in container by device in the second operation flow velocity.In some embodiments, the first operation flow velocity can fit Algae is mixed in culture vessel in permission, and the second operation flow velocity may be adapted to allow the material in the liquid in culture vessel The assimilation of material.
In some embodiments, this method can also include according at least one measurement parameter variation come change to The operation flow velocity of few second injector.In some embodiments, this method can also include at least one light source with Scheduled wavelength illumination container.
Some other aspects of the invention can be related to algae culture container spraying system.Algae culture container injection System may include at least one sensor, at least one parameter at least one sensor measurement container；At least one First fluid is assigned in container by the first injector, at least one first injector with the first operation flow velocity；And at least One the second injector, at least one second injector operate flow velocity for second with second based on the parameter that at least one is measured Fluid is assigned in container.The algae culture container spraying system can also include at least one controller, at least one control The first operation flow velocity of device control and the second operation flow velocity processed.In some embodiments, the first operation flow velocity may be adapted to allow The turbulent closure scheme algae in culture vessel, and the second operation flow velocity may be adapted to allow the material in the liquid in culture vessel Assimilation.
In some embodiments, at least one first injector can have the diameter greater than 1 millimeter.In some implementations In scheme, at least one second injector can have the diameter less than 1 millimeter.In some embodiments, scheduled fluid The group that free air and nitrogen can be selected to form.In some embodiments, algae culture container spraying system can also include Physical barriers are to separate by the fluid of the first injector and the distribution of the second injector.In some embodiments, at least one Two injectors may be configured to for carbon dioxide bubble being assigned in container.In some embodiments, at least one first First operation flow velocity of injector can be 100 mm/mins.In some embodiments, at least one the second injector First operation flow velocity can be 5 mm/mins.
Brief description
It is considered as subject of the present invention and is specifically referred and is distinctly claimed in the latter end of specification. However, when being read together with attached drawing, by reference to following detailed description, it is well understood that the present invention is about tissue and behaviour The method of work and its objects, features and advantages, in which:
Fig. 1 illustrates schematically the frame of the algae culture container spraying system of some embodiments according to the present invention Figure；
Fig. 2A illustrate schematically some embodiments according to the present invention at least one lighting unit The block diagram of algae culture container spraying system；
Fig. 2 B illustrate schematically some embodiments according to the present invention have at least one lighting unit 201 With the block diagram of the algae culture container spraying system 200 of single injector；With
Fig. 3 shows the flow chart of the method for the injection algae culture container of some embodiments according to the present invention.
It will be understood that element shown in the accompanying drawings is not drawn necessarily to scale in order to simple and clearly demonstrate.For example, being For the sake of clear, the size of some elements can be amplified relative to other elements.In addition, in the case where being deemed appropriate, ginseng The corresponding or similar element of instruction can be repeated among the figures to by examining number.
The detailed description of the embodiment of invention
In the following detailed description, numerous specific details are set forth in order to provide thorough understanding of the present invention.However, this Field the skilled person will understand that, the present invention can practice without these specific details.In other cases, do not have Well known method, program and component are described in detail, so as not to keep the present invention fuzzy.
Referring now to Figure 1, its algae culture container for illustrating schematically some embodiments according to the present invention The block diagram of spraying system 100.It should be noted that the direction of arrow can indicate the direction of information flow in Fig. 1.
In some embodiments, spraying system 100 may include at least one first spray with more than one nozzle Emitter 101, first injector 101 operate flow velocity for the first scheduled fluid (for example, air and/or nitrogen bubble) with first It is assigned in the algae culture container 10 (for example, bioreactor) of water filling, to allow to mix wherein.Spraying system 100 can also include at least one second injector 102 with more than one nozzle, and second injector 102 is with the second behaviour Make flow velocity by the second scheduled fluid (e.g., including there is CO for mass transfer2And/or the gas bubbles of the phosphorus of dissolution) It is assigned in container 10.
In some embodiments, spraying system 100 may include at least one controller 103, with the first operation of control Flow velocity and the second operation flow velocity.According to some embodiments, at least one of the first injector 101 and the second injector 102 are sprayed Mouth can be distributed a fluid in culture vessel 10 based on the request from least one controller 103, as follows further to retouch It states.In some embodiments, the first operation flow velocity can be based on the second operation flow velocity.In some embodiments, first It is scheduled for operating at least one of flow velocity and the second operation flow velocity.
In some embodiments, the first operation flow velocity may be adapted to the turbulent closure scheme for allowing algae in culture vessel 10. In some embodiments, the second operation flow velocity may be adapted to the mass transfer for allowing the material in the liquid in culture vessel 10 And/or assimilation.
In some embodiments, the second scheduled fluid may include having more than 30%CO2The gas bubbles of concentration. According to some embodiments, the source of at least one first scheduled fluid and the second scheduled fluid can be in spraying system 100 outside, such as geothermal power station can provide the carbon of dissolution and/or the source of sulphur for the second scheduled fluid.
In some embodiments, the first operation flow velocity of at least one nozzle of the first injector 101 is (for example, 100 millis M/min) can be different from the second injector 102 at least one nozzle second operation flow velocity (for example, 5 mm/mins).
In some embodiments, at least one nozzle of the first injector 101 can have straight greater than~1 millimeter Diameter.In some embodiments, at least one nozzle of the second injector 102 can have the diameter less than~1 millimeter.One In a little embodiments, the nozzle of the nozzle of the first injector 101 and the second injector 102 can distribute identical fluid (example Such as air), wherein the nozzle of each injector has different diameters.
In some embodiments, spraying system 100 can also include physical barriers 104, to be separately cultured in container 10 The first fluid distributed by the first injector 101 and the second fluid distributed by the second injector 102.In some embodiments In, at least one nozzle of the first injector 101 and/or the second injector 102 can be embedded in physical barriers 104.In In some embodiments, physical barriers 104 may be adapted to allow in predetermined (for example, upper and lower part) position of culture vessel 10 The other side (there is second fluid distribution) is flowed to from the side (distributing with first fluid) of barrier, to generate in container 10 Controlled flowing.
In some embodiments, spraying system 100 can also include at least one sensor 105 (for example, temperature sensing Device), at least one sensor 105 be coupled to controller 103 and be configured to detect in culture vessel 10 at least one A feature.For example, at least one sensor 105 can detecte, the pH in culture vessel 10 is horizontal, in temperature and pressure condition At least one.In some embodiments, at least one sensor 105 can also detect the parameter outside culture vessel 10, example As measure the gaseous emission from culture vessel 10 mass flow, with by from be inserted into container (for example, by second spray Emitter 102) amount in subtract the amount of discharge to determine the amount for the substance being absorbed into alga cells.
In some embodiments, spraying system 100 can also include at least one database 106 (or memory list Member), it is configured to store the algorithm for operating controller 103, such as the behaviour for each nozzle and/or each injector Make the database of rate.In some embodiments, spraying system 100 can also include power supply 107, which is coupled to It to controller 103 and is configured to provide electric power to spraying system 100, thus power supply 107 is suitable for at least one the first injection Device 101 and at least one second injector 102 are powered to operate at a different rate.
In some embodiments, the data collected by least one sensor 105 can pass through controller (or processing Device) 103 analyze, with detect attribute whether be more than pH in scheduled threshold value, such as container 10 horizontal and/or temperature and/or CO2The threshold value of concentration.It is more than at least one threshold in the condition (for example, as detected by sensor 105) in culture vessel 10 In the case where value, then controller 103 can operate at different flow rates the first injector 101 at least one nozzle and/or At least one nozzle of second injector 102.For example, the CO in detection container 102Concentration (or the pH water that detection is low that is more than 40% It is flat) it can cause at least one nozzle of the second injector 102 that the flow velocity of second injector 102 is reduced to~2 millis m/min Clock.In some embodiments, at least one nozzle of the second injector 102 only can receive attribute from sensor 105 More than scheduled threshold value signal when operate, and not with constant rate operation.
In some embodiments, at least one nozzle of the first injector 101 can be received only from sensor 105 Attribute be more than scheduled threshold value signal when operate, such as with the density of algae colony increase and increase mixed traffic.Root According to some embodiments, at least one nozzle of at least one nozzle of the first injector 101 and/or the second injector 102 can It is continuous thus to operate not with constant rate operation.According to some embodiments, the first injector 101 at least one At least one nozzle of nozzle and/or the second injector 102 can be operated with rate non-constant, and thus operation is nor continuous 's.
In some embodiments, culture vessel 10 can have bubbling column structure, the first injector of wherein at least one 101 are positioned on the same surface of bubble tower container at least one second injector 102.In some embodiments, it trains Supporting container 10 can have gas lift configuration, and the second injector of wherein at least one 102 is positioned in down-comer (down-comer) Bottom part, may be located remotely from sensor 105, so that the residence time of the bubble from least one the second injector 102 It can increase.
In some embodiments, spraying system 100 can permit in container 10 at least 20% organic carbon, compared to work For CO2What the carbon that bubble provides calculated.In some embodiments, at least part algae in container 10 is chlorella (Chlorella Vulgaris).In some embodiments, at least part algae in container 10 is micro- Sphaerellopsis (Nannochloropsis).In some embodiments, at least part algae in container 10 is Isochrysis galbana (Isochrysis galban)。
Referring now to Fig. 2A, illustrate schematically some embodiments according to the present invention has at least one The block diagram of the algae culture container spraying system 200 of lighting unit 201.It should be noted that the direction of arrow can indicate in Fig. 2A The direction of information flow.
In some embodiments, algae culture container spraying system 200 may include that at least one is coupled to control The lighting unit 201 of device 103, to illuminate culture vessel 10.In some embodiments, at least one lighting unit 201 and control Device 103 (or another controller) processed can be included in the bioreactor lighting system 208 for algal grown.One In a little embodiments, the distance between culture vessel 10 and at least one lighting unit 201 can be modified to control by training Support the received illumination of container 10.For example, make at least one lighting unit 201 closer to culture vessel 10, it is therein to increase The illumination of algae.In some embodiments, the distance between culture vessel 10 and at least one lighting unit 201 can be by examples It such as include the control of controller 103 in lighting system 208.According to some embodiments, in addition to or instead of change lighting unit 201 distances away from culture vessel 10 can control the illumination intensity light source 202 in lighting unit 201.
In some embodiments, at least one lighting unit 201 may include at least one light source 202 (for example, LED), it is individually controlled each light source 202 by controller 103.In some embodiments, at least one light source 202 can be controlled to the intensity light illumination different from another light source 202.According to some embodiments, all light sources 202 can be with It is controlled to change illumination intensity manually or according to the condition sensed in preset timing and/or culture vessel 10.
In some embodiments, the culture vessel 10 with physical barriers 104 may include being embedded in physical barriers At least one light source 202 (as shown in Figure 1) in 104, allows container 10 from inside, i.e., from being embedded in physics screen At least one light source 202 in barrier 104 is illuminated.According to some embodiments, culture vessel 10 may include more than one object Barrier 104 is managed, each physical barriers include at least one light source 202, allow to creation module system, wherein algae is in phase It is grown between adjacent physical barriers 104, wherein at least one controller 103, which can control, to be embedded in physical barriers 104 The illumination of all light sources 202.
It such as can be it is evident that being transported to the amount of the light of culture vessel 10 can be determined for those of ordinary skill in the art Justice is the average value for being transported to the luminous flux on surface of culture vessel 10.Therefore, for ultra high density culture (for example, density Higher than~5 grams per liters) spraying system 200, at least one lighting unit 201 can have the light point of at least one light source 202 Match, in order to provide the average light for the average flux for being substantially equal to low density cell culture object (for example, density is lower than~5 grams per liters) Flux, realizes similar light infiltration, at the same at least one lighting unit 201 each light source 202 can have it is higher strong Degree.In some embodiments, the luminous intensity in culture vessel 10 can be measured at least one sensor 105.
For example, for ultra high density culture, light-path can be short (for example,~1 millimeter -5 millimeters of illumination region And~20 millimeters -30 millimeters of dark area), allow the alga cells of proximity illumination unit 201 by Xanthophyll cycle (to algae The sublethal effect of class) and/or by photobleaching (to the lethal effect of algae), therefore lighting unit 201 can initially and container The 10 some growths maintained a certain distance to allow algae, and then closer to (for example, once a day), so as to further Increase algal grown.In some embodiments, due to short light-path, ultra high density culture may need to mix, so as to It is allowed for the illumination cycle of algae (between illumination region and dark area).In some embodiments, ultra high density is trained Feeding object can use various wavelength illuminations, because, due to short light-path, wavelength may not almost have growth under such density Have an impact.It should be noted that according to the common practice, algae is illuminated with specific wavelength (for example, with blue light), for normally raw It is long, because algae should differently respond light, however the illumination with any wavelength is had been shown by the experiment that applicant carries out It can be used for ultra high density culture.
According to some embodiments, light, which penetrates into culture vessel 10, can correspond to luminous intensity, optical wavelength, specific algae At least one of class strain and/or algal cultures density.It should be noted that light, which penetrates into, can determine culture in culture vessel 10 Distribution (ration) between illumination region and dark area in container 10, and therefore can influence by lighting unit 201 The luminous intensity of offer, by the gas flow rate of the first injector 101, pass through gas flow rate of the second injector 102 etc..
In some embodiments, culture vessel 10 can be illuminated by least one lighting unit 201, cultivated with providing It is daily more than the amount of 90% maximum algal grown in container 10.
In some embodiments, at least one lighting unit 201 may include that the low distribution of high-intensity light source 202 is matched It sets.It is such to configure the algae that can permit enhancing compared with the common practice configuration evenly distributed with low intensity light source Class growth.In some embodiments, the illumination photons flux density of at least one light source 202 is 1200 micromoles/rice2/ the second. In some embodiments, for every square metre, at least one lighting unit 201 may include at least four light sources 202.Example Such as, there is about 6 meters of surface area, the lighting unit 201 of the optical path of about 4cm may include 24 LED light sources 202, each LED light Source 202 has 1200 micromoles/rice2The luminous flux of/second.In some embodiments, at least part algae in container 10 It is Isochrysis galbana.
In some embodiments, controller 103 may be configured to control the illumination wavelengths of at least one light source 202, Such as the dedicated lighting module of the wavelength using the illumination for being suitably modified to transmitting.It in some embodiments, can be in container 27 DEG C of steady temperature is maintained in 10.
In some embodiments, controller 103 may be configured to control at least one light source 202 with 650 nanometers Wavelength illuminated.It should be noted that according to the common practice, algae is illuminated with specific wavelength (for example, with blue light), it is used for Optimum growh, however had been shown by the experiment that applicant carries out and be can be used for the illumination of other wavelength (for example, with feux rouges) The growth of enhancing.
Referring now to Fig. 2 B, illustrate schematically some embodiments according to the present invention has at least one The block diagram of the algae culture container spraying system 210 of lighting unit 201 and single third injector 211.It should be noted that in Fig. 2 B The direction of arrow can indicate the direction of information flow.
In some embodiments, spraying system 210 may include that at least one has at least one third injector 211 The lighting unit 201 of (at least one nozzle), the third injector 211 are configured to scheduled fluid being assigned to culture In container 10.In some embodiments, at least one third injector 211 may include distributing the first scheduled fluid extremely At least one nozzle (for example, there is different diameters) of the second scheduled fluid of a few nozzle and distribution.In some implementations In scheme, at least one third injector 211 may be adapted to allow the turbulent closure scheme algae in culture vessel 10, and be suitable for permitting Perhaps CO in the liquid in container 102Assimilation.
Referring now to Figure 3, it illustrates the sides of the injection algae culture container 10 of some embodiments according to the present invention The flow chart of method.In some embodiments, this method may include 301 at least one first injector 101 of control the First fluid is assigned in container 10 by one operation flow velocity.In some embodiments, this method can also include control 302 the Second fluid to be assigned in container 10 by two injectors 102 in the second operation flow velocity.In some embodiments, at least one First operation flow velocity of the first injector 101 can be different from the second operation flow velocity of at least one the second injector 102.One In a little embodiments, this method can also include at least one parameter in 303 containers 10 of measurement, and according at least one survey The parameter of amount changes to change the operation flow velocity of at least one of 304 at least one second injector 102.
In some embodiments, the first operation flow velocity may be adapted to allow the turbulent closure scheme algae in culture vessel, and And second operation flow velocity may be adapted to allow culture vessel in liquid in material assimilation.
Unless explicitly stated otherwise, otherwise method described herein embodiment is not limited to specific chronological order or the time is suitable Sequence.It in addition, during the operation order of method, can skip some in described method element, or it can be repeated .
Have been presented for various embodiments.Each of these embodiments can of course include from being presented The feature of other embodiments, and the embodiment being not specifically described may include various features described herein.

Claims (11)

1. a kind of method for spraying algae culture container, which comprises
At least one first injector is controlled so that first fluid to be assigned in the container in the first operation flow velocity；With
At least one second injector is controlled second fluid is assigned in the container to operate flow velocity second,
Wherein the first operation flow velocity is adapted to allow for mixing algae in the culture vessel, and wherein second operation Flow velocity is adapted to allow for the assimilation of the material in the liquid in the culture vessel.
2. the method as described in claim 1, further includes:
Measure at least one parameter in the container；With
Change the operation flow velocity of at least one second injector according to the variation of the parameter of at least one measurement.
3. the method as described in claim 1 further includes at least one light source with container described in scheduled wavelength illumination.
4. a kind of algae culture container spraying system, comprising:
At least one sensor, at least one parameter at least one described sensor measurement container；
First fluid is assigned to institute with the first operation flow velocity by least one first injector, at least one described first injector It states in container；
At least one second injector, at least one described second injector is based on the parameter that at least one is measured with the second operation Second fluid is assigned in the container by flow velocity；With
At least one controller, at least one controller control first operation flow velocity and the second operation flow velocity,
Wherein the first operation flow velocity is adapted to allow for the turbulent closure scheme algae in the culture vessel, and wherein described second Operation flow velocity is adapted to allow for the assimilation of the material in the liquid in the culture vessel.
5. system as claimed in claim 4, wherein at least one described first injector has the diameter greater than 1 millimeter.
6. system as described in claim 4 or 5, wherein at least one described second injector has the diameter less than 1 millimeter.
7. the system as described in any one of claim 4-6, wherein the group that scheduled fluid selects free air and nitrogen to form.
8. the system as described in any one of claim 4-7 further includes physical barriers to separate by the first injector and second The fluid of injector distribution.
9. the system as described in any one of claim 4-8, wherein at least one described second injector is configured to two Carbonoxide bubble is assigned in the container.
10. the system as described in any one of claim 4-9, wherein first behaviour of at least one first injector Making flow velocity is 100 mm/mins.
11. the system as described in any one of claim 4-10, wherein described the first of at least one second injector Operation flow velocity is 5 mm/mins.
CN201880016813.6A 2017-01-22 2018-01-18 System and method for growing algae Pending CN110506103A (en)

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