CN102826712A - Method and device for researching bio-availability of dissoluble organic nitrogen in water body - Google Patents

Method and device for researching bio-availability of dissoluble organic nitrogen in water body Download PDF

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CN102826712A
CN102826712A CN2012103257545A CN201210325754A CN102826712A CN 102826712 A CN102826712 A CN 102826712A CN 2012103257545 A CN2012103257545 A CN 2012103257545A CN 201210325754 A CN201210325754 A CN 201210325754A CN 102826712 A CN102826712 A CN 102826712A
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water
organic nitrogen
reaction tank
soluble organic
membrane
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CN102826712B (en
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霍守亮
席北斗
张靖天
苏婧
何卓识
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Chinese Research Academy of Environmental Sciences
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Chinese Research Academy of Environmental Sciences
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Abstract

The invention provides a device for researching bio-availability of dissoluble organic nitrogen in water body. A main body of a filter device is a container, a hollow fiber microfiltration membrane assembly is filled inside the container, a micropore aeration disc is installed below the membrane assembly, a bottom of a reactor is conical, and a spoil disposal port is arranged at the bottom of the reactor. Another end of the hollow fiber microfiltration membrane assembly is connected with a second water collecting tank. A main body of a dissoluble organic nitrogen extraction device is a cross flow filtration device, a water inlet of the dissoluble organic nitrogen extraction device is connected with the second water collecting tank, a concentrated solution outlet of the cross flow filtration device is connected with the second water collecting tank, and a penetrating fluid outlet is connected with a third water collecting tank. A main body of an algae growth system is a reaction tank, a water inlet of the reaction tank is connected with the second water collecting tank, a temperature sensor and a heating rod are arranged in the reaction tank, and a light source capable of adjusting luminance and a light sensor are installed on the top of the reaction tank. A circulating pump is installed outside of the reaction tank to pump water at the bottom of the reaction tank to a water surface. The invention further discloses a method for researching the bio-availability of the dissoluble organic nitrogen in the water body by using the device.

Description

The method and apparatus of the biological effectiveness of soluble organic nitrogen in the research water body
Technical field
The invention belongs to aquatic attitude research field, relate to a kind of device of studying the biological effectiveness of soluble organic nitrogen in the water body particularly.
The invention still further relates to the method for utilizing the biological effectiveness of soluble organic nitrogen in the said apparatus research water body.
Background technology
Soluble organic nitrogen (DON) is the chief component of solvability total nitrogen in the fresh water water bodys such as river, lake, wetland, on average can reach more than 60%, wherein has 12-72% directly to be utilized by biological.Overwhelming majority DON is that the DON that transfers in river mouth, the lake is important source in the fresh water water body from land infiltration, rainwash, especially people.In order to control the lake eutrophication process, many sewage works have increased denitrification process, and inorganic nitrogen is successfully removed, in any case but improve technology and improve nitration denitrification speed, final outflow water DON concentration is between 0.5-3mg/L.Many researchs show: algae has and utilizes the ability of DON compound as nitrogenous source, and some special DON component can change the group of plant plankton and constitutes, thus aggravation eutrophication process and cause a large amount of propagation of poisonous algae in the water body.Therefore, be necessary different sorts DON is carried out qualitative and quantitative examination to the influence of algal grown.
At present; Known can be had lower molecular weight DON such as free amino acid, urea by the DON that algae utilizes; And because the restriction of detection method, people to the understanding of macromole DON such as combined amino acid, enzyme, soil ulmin also seldom, thereby be difficult to the biological effectiveness of all compounds in the assessment DON storehouse.In the last few years, there was the scholar to adopt relative molecular mass to distribute to inquire into the bioavailability of algae to DON, as: technology such as dialysis, resin isolation, ultrafiltration.Dialysis method generally is applicable to the face of land water sample analysis of DIN/TDN>0.6, and cost and running time are longer; Resin isolation only limits to the separation of certain type of material, and the molecular weight span of the lyophobic dust that is produced, transitional material and hydroaropic substance is bigger, and complex operation, cost are higher; And ultra-filtration technique is through utilizing the PSPP ultra-filtration membrane to the holding back and concentrating of DON, and rejection can be separated the DON of different molecular weight more convenient, efficiently more than 90%.
Under many situations, about algae the intermittently mode of pure culture is adopted in the research of DON biological effectiveness mostly, promptly realize, and utilize the ability of organic nitrogen compound not clear in position about algae through the method that changes initial organonitrogen substrate concn.
Therefore, the present invention is used to study the ability that algae utilizes DON in position, for the biological effectiveness of inquiring into DON provides support through algal grown process under the leaching process of DON, continuous input process and the varying environment condition is combined.
Summary of the invention
The object of the present invention is to provide a kind of device of studying the biological effectiveness of soluble organic nitrogen in the water body.
Another purpose of the present invention is to provide a kind of method of utilizing the biological effectiveness of soluble organic nitrogen in the said apparatus research water body.
For realizing above-mentioned purpose, the device of the biological effectiveness of soluble organic nitrogen in the research water body provided by the invention mainly comprises filtration unit, soluble organic nitrogen extraction element and algal grown system; Wherein:
The main body of filtration unit is a container, and internal tank is equipped with the hollow fiber microfiltration membrane assembly, and the micro-pore aeration dish is equipped with in membrane module below, and reactor bottom is for conical and be provided with mud discharging mouth, be used for reactor drum when out of service deposition discharge the bigger PM for particulate matter of density; The hollow fiber microfiltration membrane assembly the other end connects second water collecting basin;
The main body of soluble organic nitrogen extraction element is a cross-flow filtration device, is used for concentrating the soluble organic nitrogen of PSPP; The water-in of cross-flow filtration device connects second water collecting basin, and the concentrated solution outlet of cross-flow filtration device is connected to second water collecting basin, and the penetrating fluid outlet is connected to the 3rd water collecting basin;
The algal grown system body is a reaction tank, is equipped with in this reaction tank through being added with the former water of algae after the sterilization; The water-in of this reaction tank is connected with second water collecting basin, establishes TP and heating rod in this reaction tank, intensity adjustable light source and optical sensor that the top of reaction tank is equipped with; The reaction tank outside is equipped with recycle pump, and the reaction tank bottom water is evacuated to the water surface.
Described device, wherein, the hollow fiber microfiltration membrane assembly is made up of film silk and cylinder, and film silk two ends are embedded in the cylinder, and the water outlet of cylinder top is connected with impeller pump, controls out water flow velocity by under meter.
Described device; Wherein, the micro-pore aeration dish is made up of diaphragm, back up pad and pneumatic duct, and the diaphragm material is terpolymer EP rubber (EPDM); One end of pneumatic duct links to each other with air; By under meter control aeration rate, the shearing force that microbubble forms can effectively be removed the pollutent attached to the film surface, prevents that fenestra from stopping up.
Described device, wherein, cross-flow filtration device is to place clean steel anchor clamps box inner by flat sheet membrane, fix with screw and constitute for four jiaos, diaphragm and anchor clamps box be shaped as circle, reduce current circulation path in anchor clamps, the raising thickening efficiency; Anchor clamps box outside is provided with water-in, concentrated solution outlet and penetrating fluid outlet, at water-in tensimeter is housed, and is used for red-tape operati pressure.
Described device, wherein, flat sheet membrane is ultra-filtration membrane or nf membrane.
Described device, wherein, the outside recycle pump of installing of the reaction tank in the algal grown system, control soluble organic nitrogen input, importing down with research different concns soluble organic nitrogen, algal grown changes.
Described device, wherein, recycle pump is when the reaction tank bottom water is evacuated to the water surface in the algal grown system; Be by under meter control flow velocity; The water outlet of recycle pump is equipped with injector, the current of discharge can all directions in reaction tank be dispersed, with hydrodynamic environment that is virtually reality like reality.
Described device wherein, is equipped with the online pH meter and the online DO appearance that adopt microcomputerized control in the reaction tank in the algal grown system, the real-time monitoring that pH and DO change in the realization algal grown process.
The method of utilizing the biological effectiveness of soluble organic nitrogen in the said apparatus research water body provided by the invention, main flow process is:
1) water sample gets into filtration unit, removes PM for particulate matter and planktonic organism through the hollow fiber microfiltration membrane effect, and the shearing force that the microbubble that the micro-pore aeration dish produces forms is removed the pollutent attached to the film surface, prevents that fenestra from stopping up;
2) water sample after the filtration gets into the soluble organic nitrogen extraction element; The cross-flow filtration device of establishing in the process adopts molecular weight cut-off any ultra-filtration membrane at 1k-100kDa; Or molecular weight cut-off is that the nf membrane of 170-300Da is carried out cross flow filter; To extract the soluble organic nitrogen of different molecular weight, soluble organic nitrogen is concentrated;
3) water sample after concentrating gets into the mode of even flow and is equipped with through being added with the algal grown system of the former water of algae after the sterilization; The kind of differing temps, illumination, water power and the algae of control algal grown system is studied the ability that algae utilizes the different molecular weight soluble organic nitrogen in position through the variation of measuring nitrogen content in the water sample.
Described method, wherein, water sample in the step 1 and Yuan Shui are meant the fresh water water body of river, lake, reservoir, and through stirring water body are mixed.
The present invention can realize simultaneously that DON extraction and algae to the original position research of its bioavailability, have the separation efficiency height, are easy to the characteristics of operation continuously, helps important Nutrition and the relevant environment effect of the deep DON of understanding to algal grown.
Description of drawings
Fig. 1 is a device synoptic diagram of the present invention.
Primary clustering nomenclature in the accompanying drawing:
1 first water collecting basin; 2 second water collecting basins; 3 the 3rd water collecting basins; 4 filtration units; 5 soluble organic nitrogen extraction elements; 6 algal grown systems; 7 air; 8 mechanical stirrers; 9,10,11 peristaltic pumps; 12 topping-up pumps; 13 recycle pumps; 14 impeller pumps; 15,16,17 under meters; 18 micro-pore aeration dishes; 19 hollow fiber microfiltration membrane assemblies; 20 hyperfiltration membrane assemblies; 21 clean steel anchor clamps; 22 tensimeters; 23 TPs; 24 optical sensors; 25 online pH meters; 26 online DO appearance; 27 heating rods; 28 incandescent light groups; 29 ASIC micro computer display panels; 30 injectors.
Embodiment
The device of the biological effectiveness of soluble organic nitrogen in the research water body provided by the invention is made up of filtration unit, soluble organic nitrogen extraction element and algal grown system.Main process comprises:
1) hollow fiber microfiltration membrane assembly and micro-pore aeration dish are housed in filtration unit, are used to remove the aperture greater than the PM for particulate matter of 0.45 μ m, planktonic organism etc.;
2) water sample after the filtration gets into the soluble organic nitrogen extraction element; In be provided with cross-flow filtration device; Adopt molecular weight cut-off to carry out cross flow filter, make that the DON of different molecular weight is concentrated in the nf membrane that any ultra-filtration membrane or the molecular weight cut-off of 1k-100kDa is 170-300Da;
3) water sample after concentrating gets into the algal grown system; This system comprises the continuous input unit of DON, temperature and illumination opertaing device, hydrodynamic simulation device, online pH and DO monitoring equipment, imports the influence to algal grown continuously in order to DON under the research varying environment condition.
The main body of described filtration unit is a cylindrical reactor, and hollow fiber microfiltration membrane assembly and micro-pore aeration dish are equipped with in inside, and reactor bottom is for conical and be provided with mud discharging mouth, be used for reactor drum when out of service deposition discharge the bigger PM for particulate matter of density etc.Membrane module is made up of film silk and square cylinder, and film silk two ends are embedded in the tube, and the tube top is provided with water outlet, is connected with impeller pump through the UPVC pipe, controls out water flow velocity by under meter.The micro-pore aeration dish is made up of diaphragm, back up pad and pneumatic duct, and an end of pneumatic duct links to each other with air, by under meter control aeration rate.Water sample gets into filtration unit through the peristaltic pump effect in first water collecting basin 1, and the other end of membrane module is connected with impeller pump through water shoot, and water outlet gets into second water collecting basin 2.
Described soluble organic nitrogen extraction element is made up of cross-flow filtration device, peristaltic pump, topping-up pump etc.; Water sample in the water collecting basin 2 gets into cross-flow filtration device behind peristaltic pump, topping-up pump; Utilize the flow of peristaltic pump to regulate intake pressure; Liquid concentrator is back to second water collecting basin 2, and penetrating fluid is collected by the 3rd water collecting basin 3.Between peristaltic pump and the topping-up pump, between topping-up pump and the water-in, between concentrated solution outlet and second water collecting basin 2, be connected by silicone tube between penetrating fluid outlet and the 3rd water collecting basin 3.
Described cross-flow filtration device is made up of flat sheet membrane, clean steel anchor clamps box, tensimeter, valve, silicone tube etc.; Flat sheet membrane is ultra-filtration membrane or nf membrane; It is inner that flat sheet membrane is installed in clean steel anchor clamps box; Water-in, concentrated solution outlet and penetrating fluid outlet are arranged respectively at outside two ends of anchor clamps and bottom, are connected to respectively in the container separately.The water-in front end connects tensimeter through silicone tube, is used for red-tape operati pressure.
Described algal grown system body is that volume is the synthetic glass rectangular parallelepiped of 80L, in be equipped with 60L through the sterilization former water, mainly comprise: the continuous input unit of DON, temperature and illumination opertaing device, hydrodynamic simulation device, online pH and DO watch-dog.
The continuous input unit of described DON is made up of accurate peristaltic pump and the silicone tube that can accurately control flow velocity, and controlling fast scope is 0.02-0.2ml/min.
Described temperature and illumination opertaing device come controlled temperature and intensity adjustable incandescent light that is fixed on the system top and optical sensor control illumination by TP and heating rod.
Described hydrodynamic simulation device is made up of recycle pump, under meter, injector, UPVC pipe fitting.Manage through UPVC between one end of recycle pump and the system bottom, the other end and under meter, under meter and injector and be connected.
Described online pH and DO watch-dog are by microcomputerized control.
Described TP, optical sensor, online pH and DO watch-dog are connected with the ASIC micro computer display panel through circuit.
The present invention utilizes the method for the biological effectiveness of soluble organic nitrogen in the said apparatus research water body, and main flow process is:
1) water sample gets into the secondary filter device, removes PM for particulate matter, planktonic organism etc. through 0.45 μ m hollow fiber microfiltration membrane effect, and the micro-pore aeration dish can produce a large amount of microbubbles, and formed shearing force can effectively be removed the pollutent attached to the film surface, prevents that fenestra from stopping up.
2) water sample after the filtration gets into the DON extraction element; In be provided with cross-flow filtration device; Adopt molecular weight cut-off to carry out cross flow filter in the nf membrane that any ultra-filtration membrane or the molecular weight cut-off of 1k-100kDa is 170-300Da; To extract the DON of different molecular weight,, make that DON is concentrated like amino acid, enzyme, polypeptide and soil ulmin etc.
3) water sample after concentrating gets into the algal grown system with the mode of even flow, studies the ability that algae utilizes different molecular weight DON in position through controlled temperature, illumination, hydrodynamic variation.
Described method, wherein, the hollow fiber microfiltration membrane material is that pvdf (PVDF), aperture are that 0.45 μ m, internal diameter are 1.4mm, long 80cm.
Described method, wherein, the micro-pore aeration dish is made up of diaphragm, back up pad and pneumatic duct, and the diaphragm material is terpolymer EP rubber (EPDM), and bubble size is 1.0-1.3mm, and air demand is 1.8m 3/ h, service area are 0.6-0.9m 2
Described method, wherein, the used flat sheet membrane of cross-flow filtration device is ultra-filtration membrane or nf membrane, its surface-area is 0.15m 2, working pressure is 0.2-0.7MPa, and the enrichment water yield is 10-15L, and the water sample cycles of concentration is 6-10 times.
Described method, wherein, hydrodynamic variation is by placing the outside recycle pump of system; The water of bottom is evacuated to the water surface, and by under meter control flow velocity, water outlet is equipped with injector; Guaranteeing to discharge current can all directions disperse in system, with hydrodynamic environment that is virtually reality like reality.
Below in conjunction with accompanying drawing the present invention is done further description.
The water body of indication of the present invention is fresh water water bodys such as river, lake, reservoir.
As shown in Figure 1, the device of the biological effectiveness of soluble organic nitrogen in the research water body provided by the invention comprises filtration unit, soluble organic nitrogen extraction element and algal grown system:
Filtration unit: constitute by hollow fiber microfiltration membrane assembly and micro-pore aeration dish; The water outlet of membrane module is connected with impeller pump through under meter through the UPVC pipe; Water outlet gets into second water collecting basin 2; Bottom of device is provided with the micro-pore aeration dish, and the other end of gas port links to each other with air through under meter through silicone tube.
Soluble organic nitrogen extraction element: for being the cross-flow filtration device of main body with the plate film assembly; Water sample in second water collecting basin 2 gets into cross-flow filtration device through peristaltic pump, topping-up pump effect; By tensimeter red-tape operati pressure; Liquid concentrator is back to second water collecting basin 2 through silicone tube, and penetrating fluid is collected by the 3rd water collecting basin 3 through silicone tube, and cycles of concentration is 6-10 times.
Algal grown system: be continuously pumped into the algal grown system through accurate peristaltic pump by water sample in second water collecting basin 2 after concentrating; Wherein, between heating rod and the ASIC micro computer display panel, between optical sensor and the ASIC micro computer display panel, between online pH meter and the ASIC micro computer display panel, be connected by circuit between online DO appearance and the ASIC micro computer display panel.
The device of the biological effectiveness of soluble organic nitrogen in the research water body provided by the invention relates to filtration unit, soluble organic nitrogen extraction element and the algal grown system of a series operation.Explanation is as follows respectively:
(1) water sample after the collection gets into first water collecting basin 1, establishes mechanical stirrer 8 in the pond and guarantees that water body mixes, and by peristaltic pump 9 water sample is pumped into filtration unit 4, is provided with hollow fiber microfiltration membrane assembly 19 and micro-pore aeration dish 18 in the filtration unit 4.Hollow fiber microfiltration membrane assembly 19 is that 0.45 μ m, internal diameter are that 1.4mm, long 80cm film silk and material are that the square cylinder of UPVC constitutes by the some amount aperture, and film silk two ends are embedded in the tube, and the tube top is provided with water outlet and is connected with impeller pump 14 through the UPVC pipe.Water sample is removed PM for particulate matter, planktonic organism etc. through the filteration of microfiltration membrane, gets in the square cylinder, is pumped into (by the under meter 16 control water yields) in second water collecting basin 2 by impeller pump 14.The micro-pore aeration dish 18 of bottom of device can produce the bubble that is of a size of 1.0-1.3mm, and formed shearing force can effectively be removed the pollutent on film surface, prevents that film from stopping up, and the other end of micro-pore aeration dish links to each other with air 7 through under meter 15 through pneumatic duct.
(2) get into second water collecting basin 2 through pretreated water sample; Effect through peristaltic pump 10 and topping-up pump 12 gets into soluble organic nitrogen extraction element 5; Comprise flat plate ultrafiltration membrane 20, clean steel anchor clamps box 21 and tensimeter 22 in the soluble organic nitrogen extraction element 5; Flat plate ultrafiltration membrane 20 usefulness screws are fixed in the clean steel anchor clamps box 21, by tensimeter 22 red-tape operati pressure.Used flat plate ultrafiltration membrane is that molecular weight cut-off is that any ultra-filtration membrane or the molecular weight cut-off of 1k-100kDa is the nf membrane of 170-300Da, and the film surface-area is 0.15m 2, working pressure is 0.2-0.7Mpa.Penetrating fluid gets into the 3rd water collecting basin 3 and collects, and liquid concentrator is back to second water collecting basin 2, and behind 2-4 hour cross flow filter, the DON volume is concentrated 6-10 doubly.
(3) got into algal grown system 6 by DON liquid concentrator in second water collecting basin 2 after concentrating through the effect of peristaltic pump 11; The control flow velocity is at 0.02-0.2ml/min; Algal grown system 6 main bodys are that volume is the synthetic glass rectangular parallelepiped of 80L, in be equipped with 60L through the sterilization lake water.Be provided with temperature-control device in the algal grown system 6, constitute, make that temperature remains on certain limit in the algal grown system by hot-plate 27 and TP 23.The top of algal grown system is provided with illumination control system, is made up of with optical sensor 24 4 groups of parallelly connected intensity adjustable incandescent light 28, is used for the illumination variation under the simulating natural condition.Algal grown system outside is provided with the hydrodynamic simulation device, is made up of recycle pump 13, under meter 17 and injector 30, can the water of algal grown system bottom be evacuated to the water surface, realizes the diffuse transmission type ejection by injector 30, guarantees to mix.In the algal grown system, be provided with online pH meter 25 and online DO appearance 26, with the variation of external environmental condition in the monitoring algal grown process.These on-line equipments link to each other with ASIC micro computer display panel 29, can Real Time Observation to the variation of temperature, illumination, pH and DO.

Claims (10)

1. a device of studying the biological effectiveness of soluble organic nitrogen in the water body mainly comprises filtration unit, soluble organic nitrogen extraction element and algal grown system; Wherein:
The main body of filtration unit is a container, and internal tank is equipped with the hollow fiber microfiltration membrane assembly, and the micro-pore aeration dish is equipped with in membrane module below, and reactor bottom is for conical and be provided with mud discharging mouth, be used for reactor drum when out of service deposition discharge the bigger PM for particulate matter of density; The hollow fiber microfiltration membrane assembly the other end connects second water collecting basin;
The main body of soluble organic nitrogen extraction element is a cross-flow filtration device, is used for concentrating the soluble organic nitrogen of PSPP; The water-in of cross-flow filtration device connects second water collecting basin, and the concentrated solution outlet of cross-flow filtration device is connected to second water collecting basin, and the penetrating fluid outlet is connected to the 3rd water collecting basin;
The algal grown system body is a reaction tank, is equipped with in this reaction tank through being added with the former water of algae after the sterilization; The water-in of this reaction tank is connected with second water collecting basin, establishes TP and heating rod in this reaction tank, intensity adjustable light source and optical sensor that the top of reaction tank is equipped with; The reaction tank outside is equipped with recycle pump, and the reaction tank bottom water is evacuated to the water surface.
2. device according to claim 1, wherein, the hollow fiber microfiltration membrane assembly is made up of film silk and cylinder, and film silk two ends are embedded in the cylinder, and the water outlet of cylinder top is connected with impeller pump, controls out water flow velocity by under meter.
3. device according to claim 1; Wherein, the micro-pore aeration dish is made up of diaphragm, back up pad and pneumatic duct, and the diaphragm material is a terpolymer EP rubber; One end of pneumatic duct links to each other with air; By under meter control aeration rate, the shearing force that microbubble forms can effectively be removed the pollutent attached to the film surface, prevents that fenestra from stopping up.
4. device according to claim 1, wherein, cross-flow filtration device is to place clean steel anchor clamps box inner by flat sheet membrane, fix with screw and constitute for four jiaos, diaphragm and anchor clamps box be shaped as circle, reduce current circulation path in anchor clamps, the raising thickening efficiency; Anchor clamps box outside is provided with water-in, concentrated solution outlet and penetrating fluid outlet, at water-in tensimeter is housed, and is used for red-tape operati pressure.
5. device according to claim 4, wherein, flat sheet membrane is ultra-filtration membrane or nf membrane.
6. device according to claim 1, wherein, the outside recycle pump control soluble organic nitrogen input of installing of the reaction tank in the algal grown system, importing down with research different concns soluble organic nitrogen, algal grown changes.
7. according to claim 1 or 6 described devices; Wherein, Recycle pump is that the water outlet of recycle pump is equipped with injector by under meter control flow velocity when the reaction tank bottom water is evacuated to the water surface in the algal grown system; The current of discharge can all directions in reaction tank be dispersed, with hydrodynamic environment that is virtually reality like reality.
8. device according to claim 7 wherein, is equipped with the online pH meter and the online DO appearance that adopt microcomputerized control in the reaction tank in the algal grown system, the real-time monitoring that pH and DO change in the realization algal grown process.
9. method of utilizing the biological effectiveness of soluble organic nitrogen in the said device of the claim 1 research water body, main flow process is:
1) water sample gets into filtration unit, removes PM for particulate matter and planktonic organism through the hollow fiber microfiltration membrane effect, and the shearing force that the microbubble that the micro-pore aeration dish produces forms is removed the pollutent attached to the film surface, prevents that fenestra from stopping up;
2) water sample after the filtration gets into the soluble organic nitrogen extraction element; The cross-flow filtration device of establishing in the process adopts molecular weight cut-off any ultra-filtration membrane at 1k-100kDa; Or molecular weight cut-off is that the nf membrane of 170-300Da is carried out cross flow filter; To extract the soluble organic nitrogen of different molecular weight, soluble organic nitrogen is concentrated;
3) water sample after concentrating gets into the mode of even flow and is equipped with through being added with the algal grown system of the former water of algae after the sterilization; The kind of differing temps, illumination, water power and the algae of control algal grown system is studied the ability that algae utilizes the different molecular weight soluble organic nitrogen in position through the variation of measuring nitrogen content in the water sample.
10. method according to claim 9, wherein, water sample in the step 1 and Yuan Shui are meant the fresh water water body of river, lake, reservoir, and through stirring water body are mixed.
CN201210325754.5A 2012-09-05 2012-09-05 Method and device for researching bio-availability of dissoluble organic nitrogen in water body Active CN102826712B (en)

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CN104150686A (en) * 2014-07-18 2014-11-19 湖北百清环保技术有限公司 Landfill leachate treatment method based on microporous aeration ultrafiltration and efficient adsorption
CN105784954A (en) * 2014-12-16 2016-07-20 中国科学院生态环境研究中心 Experiment apparatus for simulating river organic nitrogen mineralization
CN106771043A (en) * 2017-01-20 2017-05-31 南京大学 A kind of quick method for analyzing water-soluble organic nitrogen biological effectiveness
CN107473384A (en) * 2016-06-07 2017-12-15 中国石油化工股份有限公司 A kind of device and method that ammonia nitrogen waste water is handled using microalgae
CN107473383A (en) * 2016-06-07 2017-12-15 中国石油化工股份有限公司 A kind of device and method for handling ammonia nitrogen waste water
CN107473494A (en) * 2016-06-07 2017-12-15 中国石油化工股份有限公司 The device and method of ammonia nitrogen in a kind of removal waste water
CN107655842A (en) * 2017-09-19 2018-02-02 南京大学 A kind of degradable soluble organic nitrogen assay method of saprobia based on static culture
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CN107473494B (en) * 2016-06-07 2020-11-10 中国石油化工股份有限公司 Device and method for removing ammonia nitrogen in wastewater
CN107473383B (en) * 2016-06-07 2020-11-10 中国石油化工股份有限公司 Device and method for treating ammonia nitrogen wastewater
CN107473384B (en) * 2016-06-07 2020-07-24 中国石油化工股份有限公司 Device and method for treating ammonia nitrogen wastewater by using microalgae
CN106771043A (en) * 2017-01-20 2017-05-31 南京大学 A kind of quick method for analyzing water-soluble organic nitrogen biological effectiveness
CN106771043B (en) * 2017-01-20 2019-05-21 南京大学 A method of quickly analyzing water-soluble organic nitrogen biological effectiveness
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CN107655842B (en) * 2017-09-19 2019-11-05 南京大学 A kind of degradable soluble organic nitrogen measuring method of saprobia based on static culture
CN107655842A (en) * 2017-09-19 2018-02-02 南京大学 A kind of degradable soluble organic nitrogen assay method of saprobia based on static culture

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